LOCUS       DAJSNV010000001        84939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSNV010000001 DAJSNV010000000
VERSION     DAJSNV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.001"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(118..1605)
                     /locus_tag="ON516_000001"
     CDS             complement(118..1605)
                     /locus_tag="ON516_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343520.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(2160..2723)
                     /locus_tag="ON516_000002"
     CDS             complement(2160..2723)
                     /locus_tag="ON516_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3343521.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2738..3781)
                     /locus_tag="ON516_000003"
     CDS             complement(2738..3781)
                     /locus_tag="ON516_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343522.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3914..4318)
                     /locus_tag="ON516_000004"
     CDS             complement(3914..4318)
                     /locus_tag="ON516_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3343523.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4551..5186)
                     /locus_tag="ON516_000005"
     CDS             complement(4551..5186)
                     /locus_tag="ON516_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3343524.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5452..6504)
                     /locus_tag="ON516_000006"
     CDS             complement(5452..6504)
                     /locus_tag="ON516_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3343525.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6517..7455)
                     /locus_tag="ON516_000007"
     CDS             complement(6517..7455)
                     /locus_tag="ON516_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3343526.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7906..8556)
                     /locus_tag="ON516_000008"
     CDS             complement(7906..8556)
                     /locus_tag="ON516_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343527.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8656..9165)
                     /locus_tag="ON516_000009"
     CDS             complement(8656..9165)
                     /locus_tag="ON516_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3343528.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9250..9729)
                     /gene="rlmH"
                     /locus_tag="ON516_000010"
     CDS             complement(9250..9729)
                     /gene="rlmH"
                     /locus_tag="ON516_000010"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3343529.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10157..11446
                     /locus_tag="ON516_000011"
     CDS             10157..11446
                     /locus_tag="ON516_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3343530.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(11485..12090)
                     /locus_tag="ON516_000012"
     CDS             complement(11485..12090)
                     /locus_tag="ON516_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3343531.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(12295..12759)
                     /locus_tag="ON516_000013"
     CDS             complement(12295..12759)
                     /locus_tag="ON516_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3343532.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(12781..13101)
                     /locus_tag="ON516_000014"
     CDS             complement(12781..13101)
                     /locus_tag="ON516_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3343533.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(13245..14324)
                     /gene="pepA"
                     /locus_tag="ON516_000015"
     CDS             complement(13245..14324)
                     /gene="pepA"
                     /locus_tag="ON516_000015"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3343534.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14524..14874
                     /locus_tag="ON516_000016"
     CDS             14524..14874
                     /locus_tag="ON516_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3343535.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            15097..16044
                     /locus_tag="ON516_000017"
     CDS             15097..16044
                     /locus_tag="ON516_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3343536.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(16101..17519)
                     /gene="pepV"
                     /locus_tag="ON516_000018"
     CDS             complement(16101..17519)
                     /gene="pepV"
                     /locus_tag="ON516_000018"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3343537.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            17668..18513
                     /locus_tag="ON516_000019"
     CDS             17668..18513
                     /locus_tag="ON516_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3343538.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18517..18957
                     /locus_tag="ON516_000020"
     CDS             18517..18957
                     /locus_tag="ON516_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3343539.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            19007..19201
                     /locus_tag="ON516_000021"
     CDS             19007..19201
                     /locus_tag="ON516_000021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343540.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            19337..20023
                     /locus_tag="ON516_000022"
     CDS             19337..20023
                     /locus_tag="ON516_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3343541.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(20431..21120)
                     /locus_tag="ON516_000023"
     CDS             complement(20431..21120)
                     /locus_tag="ON516_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343542.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(21095..23224)
                     /locus_tag="ON516_000024"
     CDS             complement(21095..23224)
                     /locus_tag="ON516_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3343543.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(23272..24609)
                     /locus_tag="ON516_000025"
     CDS             complement(23272..24609)
                     /locus_tag="ON516_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3343544.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(24861..25220)
                     /gene="rplT"
                     /locus_tag="ON516_000026"
     CDS             complement(24861..25220)
                     /gene="rplT"
                     /locus_tag="ON516_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3343545.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(25273..25473)
                     /gene="rpmI"
                     /locus_tag="ON516_000027"
     CDS             complement(25273..25473)
                     /gene="rpmI"
                     /locus_tag="ON516_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3343546.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(25545..26069)
                     /gene="infC"
                     /locus_tag="ON516_000028"
     CDS             complement(25545..26069)
                     /gene="infC"
                     /locus_tag="ON516_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3343547.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(26113..26238)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26389..27021
                     /locus_tag="ON516_000029"
     CDS             26389..27021
                     /locus_tag="ON516_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3343548.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(27023..27928)
                     /locus_tag="ON516_000030"
     CDS             complement(27023..27928)
                     /locus_tag="ON516_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3343549.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(28013..28672)
                     /locus_tag="ON516_000031"
     CDS             complement(28013..28672)
                     /locus_tag="ON516_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3343550.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            28988..29932
                     /gene="mvk"
                     /locus_tag="ON516_000032"
     CDS             28988..29932
                     /gene="mvk"
                     /locus_tag="ON516_000032"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3343551.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            29925..30902
                     /gene="mvaD"
                     /locus_tag="ON516_000033"
     CDS             29925..30902
                     /gene="mvaD"
                     /locus_tag="ON516_000033"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3343552.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            30915..32000
                     /locus_tag="ON516_000034"
     CDS             30915..32000
                     /locus_tag="ON516_000034"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3343553.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            31997..33061
                     /locus_tag="ON516_000035"
     CDS             31997..33061
                     /locus_tag="ON516_000035"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3343554.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(33310..34113)
                     /locus_tag="ON516_000036"
     CDS             complement(33310..34113)
                     /locus_tag="ON516_000036"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3343555.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(34133..34975)
                     /gene="kduI"
                     /locus_tag="ON516_000037"
     CDS             complement(34133..34975)
                     /gene="kduI"
                     /locus_tag="ON516_000037"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3343556.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(34991..35998)
                     /locus_tag="ON516_000038"
     CDS             complement(34991..35998)
                     /locus_tag="ON516_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3343557.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(36005..36649)
                     /locus_tag="ON516_000039"
     CDS             complement(36005..36649)
                     /locus_tag="ON516_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3343558.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(36749..37507)
                     /locus_tag="ON516_000040"
     CDS             complement(36749..37507)
                     /locus_tag="ON516_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3343559.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(37634..38785)
                     /gene="fucO"
                     /locus_tag="ON516_000041"
     CDS             complement(37634..38785)
                     /gene="fucO"
                     /locus_tag="ON516_000041"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3343560.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(38867..39178)
                     /gene="rhaM"
                     /locus_tag="ON516_000042"
     CDS             complement(38867..39178)
                     /gene="rhaM"
                     /locus_tag="ON516_000042"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR3343561.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(39180..40025)
                     /gene="rhaD"
                     /locus_tag="ON516_000043"
     CDS             complement(39180..40025)
                     /gene="rhaD"
                     /locus_tag="ON516_000043"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR3343562.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(40039..41295)
                     /gene="rhaA"
                     /locus_tag="ON516_000044"
     CDS             complement(40039..41295)
                     /gene="rhaA"
                     /locus_tag="ON516_000044"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR3343563.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(41292..42752)
                     /gene="rhaB"
                     /locus_tag="ON516_000045"
     CDS             complement(41292..42752)
                     /gene="rhaB"
                     /locus_tag="ON516_000045"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR3343564.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            42905..43774
                     /locus_tag="ON516_000046"
     CDS             42905..43774
                     /locus_tag="ON516_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR3343565.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(43900..44799)
                     /locus_tag="ON516_000047"
     CDS             complement(43900..44799)
                     /locus_tag="ON516_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3343566.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(44964..45788)
                     /locus_tag="ON516_000048"
     CDS             complement(44964..45788)
                     /locus_tag="ON516_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3343567.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(45799..47094)
                     /locus_tag="ON516_000049"
     CDS             complement(45799..47094)
                     /locus_tag="ON516_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR3343568.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(47110..47589)
                     /locus_tag="ON516_000050"
     CDS             complement(47110..47589)
                     /locus_tag="ON516_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3343569.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(47595..48623)
                     /locus_tag="ON516_000051"
     CDS             complement(47595..48623)
                     /locus_tag="ON516_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3343570.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(48640..49746)
                     /locus_tag="ON516_000052"
     CDS             complement(48640..49746)
                     /locus_tag="ON516_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3343571.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(49975..52572)
                     /gene="adhE"
                     /locus_tag="ON516_000053"
                     /gene_synonym="adhC"
     CDS             complement(49975..52572)
                     /gene="adhE"
                     /locus_tag="ON516_000053"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3343572.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(52877..53257)
                     /gene="yajC"
                     /locus_tag="ON516_000054"
     CDS             complement(52877..53257)
                     /gene="yajC"
                     /locus_tag="ON516_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3343573.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(53354..54499)
                     /gene="tgt"
                     /locus_tag="ON516_000055"
     CDS             complement(53354..54499)
                     /gene="tgt"
                     /locus_tag="ON516_000055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3343574.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            54717..55415
                     /locus_tag="ON516_000056"
     CDS             54717..55415
                     /locus_tag="ON516_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3343575.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(55583..58222)
                     /locus_tag="ON516_000057"
     CDS             complement(55583..58222)
                     /locus_tag="ON516_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3343576.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(58841..59872)
                     /gene="queA"
                     /locus_tag="ON516_000058"
     CDS             complement(58841..59872)
                     /gene="queA"
                     /locus_tag="ON516_000058"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3343577.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            60065..61240
                     /locus_tag="ON516_000059"
     CDS             60065..61240
                     /locus_tag="ON516_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3343578.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            61244..61885
                     /locus_tag="ON516_000060"
     CDS             61244..61885
                     /locus_tag="ON516_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343579.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            61882..62799
                     /locus_tag="ON516_000061"
     CDS             61882..62799
                     /locus_tag="ON516_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3343580.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            62796..63467
                     /locus_tag="ON516_000062"
     CDS             62796..63467
                     /locus_tag="ON516_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343581.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(64008..64496)
                     /locus_tag="ON516_000063"
     CDS             complement(64008..64496)
                     /locus_tag="ON516_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3343582.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(64697..64963)
                     /locus_tag="ON516_000064"
     CDS             complement(64697..64963)
                     /locus_tag="ON516_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3343583.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(64963..65226)
                     /locus_tag="ON516_000065"
     CDS             complement(64963..65226)
                     /locus_tag="ON516_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3343584.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            65702..65887
                     /locus_tag="ON516_000066"
     CDS             65702..65887
                     /locus_tag="ON516_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3343585.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(66029..67852)
                     /locus_tag="ON516_000067"
     CDS             complement(66029..67852)
                     /locus_tag="ON516_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3343586.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(68450..71155)
                     /locus_tag="ON516_000068"
     CDS             complement(68450..71155)
                     /locus_tag="ON516_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3343587.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(71461..72408)
                     /locus_tag="ON516_000069"
     CDS             complement(71461..72408)
                     /locus_tag="ON516_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3343588.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            72598..72867
                     /gene="rpsN"
                     /locus_tag="ON516_000070"
     CDS             72598..72867
                     /gene="rpsN"
                     /locus_tag="ON516_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3343589.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(73043..73405)
                     /locus_tag="ON516_000071"
     CDS             complement(73043..73405)
                     /locus_tag="ON516_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3343590.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(73416..74537)
                     /locus_tag="ON516_000072"
     CDS             complement(73416..74537)
                     /locus_tag="ON516_000072"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3343591.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(74553..74903)
                     /locus_tag="ON516_000073"
     CDS             complement(74553..74903)
                     /locus_tag="ON516_000073"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3343592.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(75033..76544)
                     /locus_tag="ON516_000074"
     CDS             complement(75033..76544)
                     /locus_tag="ON516_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3343593.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(76866..78218)
                     /locus_tag="ON516_000075"
     CDS             complement(76866..78218)
                     /locus_tag="ON516_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3343594.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(78371..79447)
                     /locus_tag="ON516_000076"
     CDS             complement(78371..79447)
                     /locus_tag="ON516_000076"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3343595.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            79643..81388
                     /locus_tag="ON516_000077"
     CDS             79643..81388
                     /locus_tag="ON516_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3343596.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(81668..82105)
                     /locus_tag="ON516_000078"
     CDS             complement(81668..82105)
                     /locus_tag="ON516_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3343597.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(82089..83462)
                     /locus_tag="ON516_000079"
     CDS             complement(82089..83462)
                     /locus_tag="ON516_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343598.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(83784..84272)
                     /locus_tag="ON516_000080"
     CDS             complement(83784..84272)
                     /locus_tag="ON516_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3343599.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(84294..84896)
                     /locus_tag="ON516_000081"
     CDS             complement(84294..84896)
                     /locus_tag="ON516_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3343600.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        24636 a        18078 c        15307 g        26918 t
ORIGIN      
        1 ctgataagaa aactaattaa aagaaagagg taactacata aactaattaa aaaagctaac
       61 ttcatagttg gaggataaat ggaagaaact tcaatgattg tttctccttg tgttccttca
      121 atcgacacta gtcctattat caatctctct tttttgtcca tttacttttg cgactgttcc
      181 ccaataatca aatacaggaa gcgttatttt ttgtccttta gattcatgta ctgtatagac
      241 tattttcgaa tccatcactt gataaggcat agtatgccat tccttgccaa ttcttacttc
      301 atgattctgc aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt cataaagtcc
      361 tccgggaagg agtgtcgtgt aacgaataag attttcttga ttcattacca agtgatctct
      421 ctgagtgaca actttatgtt catagtttgc ttttgttgca gcagatacag ccaacagtaa
      481 taaaacaatt gccgttatat taactacttt tctttgctta gctgatgtgc tcttcatgaa
      541 aacgaacgat aaaatcaacg agccaaacag tacttgaaag cctaagattc gccaaggaaa
      601 ttgaatcatt tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt tggtggataa
      661 gagccaagag actaccgcac taaggtaaat cactttttct ttgcgattaa atcctttaaa
      721 aaagaagagt ggacagatca aagaaataat accaatgatt cctaccgtaa aggttgtcag
      781 gtcattattc atcgcaccaa tcaaactatc cgagacattt aatgcttggt gttgtaaggt
      841 cgttttatct ggctcattaa tcgattgaac catgatttgt cggaacatcg gataccaaaa
      901 gtatgctgtt aaaagaatcg tcattcctac tgcctttgac aactccacaa tcaaccggat
      961 gctttctttt tttcttgttt cacttactac aaagccgcct aagactagta agccaacggc
     1021 tattacactc ataaaagtgg aaagaacatg tgtataaatg agtaagctca tactgactgc
     1081 taatggaatt gcacatggct ttttctcata aatcagtgcg taagcataat acataactat
     1141 cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa tatcaatcgt
     1201 acggtaccca taaaatgtat ataaacagct aaatataaaa gccgcatcga ttctttttaa
     1261 aaattttagt ccataatgaa acatcagcat gatagtacaa acattcaata gccaaacata
     1321 taagatataa ctaacgatta aattattact tatccaataa aaaatgactg ccggaaaaaa
     1381 ggtcaagtag ggataaaaat aattcactcc gctcccataa tgattaaacg tcgtaaaatt
     1441 gattggacca gaaaaaattg aagataaccc tttgactcta gcaaaatgga aggcaatatc
     1501 ccccgatgta gaaatagtat gaacatttct aaaataattg attgaataaa gtccagcaat
     1561 aattgctagg agaatggaca atattatttt tttctttact tgcattactt aattatatac
     1621 tcctttcatg gattgcttta tctagtataa cttaaatttt aaataaatcg ttatttattt
     1681 ttactctcac ttaaaaaagc actaaaatta taagagaggg aggtaatggt ctgaacacca
     1741 agaattgttc caacatcgaa ttgcttaact aatgagttca aagcttaatt atgtggctct
     1801 tatatttacc gagcgtggat tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa
     1861 acagccatat ccgctttctt ggatatgact atttactact actttactat ctttcattct
     1921 tcttgtctca cttatgataa ggcgctgcgt atcatgagtg aatgatatta ggaaaacttc
     1981 ataaaataaa tcttttatac atttccactt ctagatggtc attcatgtta gaagaatcaa
     2041 actatttttt tactgtcgta ataatattaa aacactagaa caaatcaatt aaattccata
     2101 aaatataaat aatagaccta gcatcatctg gtataaaatg gatgctaggt ccgttatatt
     2161 tactcaaaat tgattctacg aatagatctt cttatcattt ctattttagt agtcatgcgt
     2221 tgttgttctt ctaaaggtgg taatggtata agtagtttag ataaatttgt accatttaca
     2281 tttggttgcc cagttccgta agaaattttt tctaataact tccaatataa tggcgagtct
     2341 aagaagcagt ctacgtattc tgataataat ttctcaacta acctaattcg aatcaagtaa
     2401 gaagcaaaaa cagattcctc acttatttcc ttaaccaaga aactttttcc catagttcct
     2461 cctgtccttg ctatcaaaat gtcattttct tccaatctta aatcaactag tttagaatta
     2521 ctaatatcac agtaaggcac actggaccaa tttacacgtc cctcttgtat atctgtgatt
     2581 ctcaaaaact taacattgcc ttgttttttc gccgaatctg tatagccata ttgaatctta
     2641 gtagaaatac ttcctaaata aacccatagt cgaacatcgc tagatgattt ttcttgtcgt
     2701 tctttatgaa caacagtgat catcttgagt tgtccgacta ttttttagtt gcagcaatct
     2761 ttttgggacg cttatttgca tgaagcaacg ttgctaagtc tagtaataaa acattggcta
     2821 acacaggatt ggctttctcg cgatacgctg cggcgccact ttcaaacaat actgctagac
     2881 gatcttctac aacaacgatt tgttccaaat acggtggaaa tttgattcgt tttaggatcc
     2941 ggctaggaga gtcccgctcg gacactaaga gttccgaggt ataaggacca taagaacgac
     3001 tggctaagag ataatggcca taaaaagcca caccttgaat cttatcagat gtttttctgc
     3061 tcattgtcac ggttggttgg attttgtctt tgcggcgaat tgctgttttc atttcagcta
     3121 tcgtcggcaa tagatagaaa gtcaactgtc cttcaccttt tagtgtgaag ttgccagata
     3181 ccagttgatc attgttttga gtcaaatacg atgcttgtgg aatctctgag agatcagttg
     3241 tcagccaata agcgattggc tgtcccgtct cttcagatgt atctgctaag atatcgatca
     3301 aacgtaaagc ggctactcgt ccagtagcag atcctgtgat tgtcaaccat aaaagttccc
     3361 gttttgtatc atatactaag ccccctacat gtggccgttt aggtaaaatc agcagtttga
     3421 tcggctgtcc tgtttctcta tccagtacat gaataataga atgatgctga tggtcatagc
     3481 agtaggcaga aataaacaca aaatcttgag aaaaagccaa cccttgcggc gtcatccatt
     3541 gacaaacctc attttcgccg gtttccggca ccaccattct tgtttctgtt aatccaggaa
     3601 tgttatattg ttttgcttca gccttttccc aagctgcttg aacaatagga taccgtttta
     3661 actttttttc aatatcctga tgtatccgtt gctgcttcca atgcttaaac tttaggacat
     3721 agtaaacaag aaggccaatg accagcaaca ctaaaatcat tattgctacc cgctgtgtca
     3781 tttttttgcc tccttgttct attcgatcaa tcgtccatat accgctatta attaccgtaa
     3841 ctacatgttt cttttactag cgtttcttta taggatcttt cataatctca tttatagtaa
     3901 cattaagaat cccttattct actatagtca cttccacatc aatattgcct cttgttgctt
     3961 tagagtatgg gcaaaatgcg tgggcttctt ctgcgatttt ctctgcttgg tccttctcaa
     4021 gtcctggcaa agaaacttct aaaacgaccc cgatttggta gcccccatct tctaattgaa
     4081 ataaagatac ccgtgctttg acttcagaat cagactcaag attattttct tccagcatgt
     4141 gttgaaccgc gccgttgaag caagatgcgt atccagccgc aaaaagttgt tccggatttg
     4201 tcccttcact atggattcct gggcggtcga tcttcatgtg catacttccg tttggtgcct
     4261 caacttcgcc atcacggcct ccacgattga tcattgctgt ttcgtacatt tttttcattg
     4321 agatcatcct ttctttcgtc gttctatatt aagtgtaacg agtatttttt aatctgacaa
     4381 aggtaatgct tgcattcggc gacatcagtt agacaacatc gatgatcagt gatagaagca
     4441 gtttatctat acaaaaaaag aagggttcta tgatatttgg tgtccttact cgccttgagt
     4501 aacaacacca aatatcatag aaccacttaa tcagcttcac actgcttttt ctactttcgt
     4561 agttgaaaaa aacttaaaca aatcatcggc aaatagttaa agaaaaaact taatcgttga
     4621 aagaagccca attgcttaaa gaaggcaaac tgactaatcg acggaatccg tgctaatccg
     4681 taaagacctg caattagtaa actaataact gttaaaacaa gataaaaatg gctaagcgta
     4741 ccttgaccac tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca
     4801 tagcctagcc cagaacctat attatggatc caagtcgata gattacaaga gctttcattc
     4861 gtattgatac tgaataagcc agtaaaaata caatcaccta tcccatataa accaactgtt
     4921 gcaacaacaa gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc
     4981 aacgaaagaa caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact
     5041 ggactatcta cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa
     5101 aaaaggccaa ggatatacgg tgtcgataaa tcactcatca cacctaataa taaaaaataa
     5161 aaaccatatt tttgcaaaaa aatcattttc ttcccctact ttttagtgat tattcgatac
     5221 atccaacaat caaatttttt ctcattttaa cacatatttt tattctagtt gtcttttgaa
     5281 caatcaataa cataaaggtg agtttcttct acattttaaa aaatcgaatg gcactgaaga
     5341 gttcagttgt cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg
     5401 ttgacatctc acttacgcat ccattgattg aaaatttctt agtaaaatct actagcctaa
     5461 aaatttgaag acttcttctc gatcatacag tttaaatact ttctcgcata aaaattgagt
     5521 aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa agccatttag
     5581 ccctgcatct aaagaagcca acggaccaac aagtccgacc acgccgaaac cagcagaagc
     5641 aggcgtacct gaaatagaaa atagagcgac aggaatagct gtaatcactg cactaactaa
     5701 acaaggaata agaatcacag gttttctaaa tagattaggc atcatcatct tcatagcgcc
     5761 taaagcaatc gcaattgtca cacctgactt attaaccttc caagagttca ctactaaaac
     5821 aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca gaaacaccat ctagttggat
     5881 tgccattccg attgctactg ttgaaatagg agagataatc aaaaaggcaa aggaacaacc
     5941 aatcaagata gacattaaca gcggttgtaa ggtagtgaag ttattaatca atgatccaat
     6001 tgcagaagta attgctgtta caaaagggaa taaataaaaa ccaagtaaac ctgcacctac
     6061 acctacaaca atcggtgtta acacaatcga cactgaccca aaactatttc caatcagtaa
     6121 aatcagcccg acagcaatcg atgctgtcag catcgtatta ataatatcgc ctgtaccagc
     6181 accaacgtaa acacccgttt cacccaaatc aggtgtgaat ttgactacac cagaaccaac
     6241 aaaagaagct ccggctacaa ccatcattct catcggatca aatccaaatt gtaaggcaat
     6301 caaaccacca atgatcaaag gtgttgctag ttgaaaaata actgctaact gtgcgatcgt
     6361 taccgcaaag ggatacgcac taaaatattt taaaatggca cctaatactg cattaggaat
     6421 caatccaata atgattcctg ttgctgtacc agcgaggact ttattcagaa aaattttcgg
     6481 tgatattttt tgtgaagttt ccattcaatc tctcctttat tttacgttca aactatcttc
     6541 ttttgcatgg acaagctcag tcaagaaatc acaataaggc gtcgcaacac catatttctt
     6601 cccttttcta gaaattgcgc cattgatata atcgatttct gttaatcgat ggttcttaat
     6661 caagtcttga tacattgaag gataatgtaa cccaattgtt tccgggtcaa aacaagattc
     6721 acaatgtgca atgacttctg ggacatctag ttcaatcttc tctactgctg ctactttggc
     6781 aaactcattg acaatcgtcg ccaccatttt atgagcagtc gatgtttttc ctagctcagc
     6841 catattaacg tctaagattg tgcatagtcc gttcattgtt ccgttcacac atgctttgcg
     6901 ataaatagaa tagtgaatat tgtcagaaaa atgagcattt aatcctgatt cagacaattt
     6961 gtctgctaat ttttttgcag ctgcttcttt tccatcacct aaattttgta attctactga
     7021 accacttcca aataatttga cctgaccagg accttcaaga cctgccgtcc acatggtatt
     7081 tccaatatag atattttcca ttggtacaaa tttctcaata atatcttcat gcccgatacc
     7141 atttaataga cataagacct ctgtatcttt tttgattaat gattggatat cctgcagcat
     7201 tttttccagc tgcatcgctt tggtaaatag aataatcaga tcaacttgat cttctttttc
     7261 tacttcggat tgaagaacga ttggtagttt tgcttctacc tctttaccat taaaatttgc
     7321 ttgtaatcca tgttctttga tttgttggac atgttctgcc cagccatcga ttaataatac
     7381 ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt cccattgcgc cagcgcctgc
     7441 aattgctatt ttcattgatt cttcctcctt tttctttcta ttatactcat ttgagaatct
     7501 tttcacaacc tcgttttgtt attcttctct tatttatcat gtttatatcc accattcaag
     7561 ttattttatc agcaaatcaa taaaaactgg aatagaatcc caatttcatc gtaagagaaa
     7621 ctagactagc aataagaaaa tagaaacatt catagaaaaa atctcactcc tctgattata
     7681 aaagaggggg cattaacctc gtgtatacta aaaccttcca ttcattcgat ggatgatttt
     7741 ctttttgtga aaaaatattc attgtagtac gatgttgcgc aacacagacg gatggtattg
     7801 cttattattt attaaaatcg aacaatacac ttatttaaaa acttctttcc cagattttct
     7861 tccattttta cagttataac tagtattatc atgaaaaata gccttttatt gtctatggga
     7921 ataataattt aaaattggta cttttttatt aattagttca tcgcccaata atctctccag
     7981 tttcttagca tctcctgact caaccggaat tatccaagcc cagtctaaat aattttttaa
     8041 aatctcatca tattttgcaa atatttcatt gccatattcc gtccttaaaa ttatattttc
     8101 agtgccagac aagtgattta tattgagttg ataaaaaaga tctccatcta aaaaaatatc
     8161 attagaagaa gatttcccca cagtaatgac cattccatca gctctatgtg ttacatacaa
     8221 ataattgtat gacctcattt cacctaatga atcaattatt tcttgccctt tagctctaat
     8281 atattgatct ctattaccta tctcactctt atttaacaaa agttttttca tgtcaaattt
     8341 aatcggctgt tttatttttg taaattcgct tctactacta atccaatccg agattaattt
     8401 ttcaacatgt cccagagtaa actctgattt attcgctaaa tacttttttt gttgtattaa
     8461 agacatcttt ccccgaagct caaaattgag cctacatcga ttctctatct cttcaaaaag
     8521 catcttatct tcatcattct tatattcatc attcataaaa ccaatccagc tcctcgatta
     8581 agataatatt ttaatctcat acaaaaacta gctctcatca aaacaaccat tgttttgatt
     8641 tctcatgaaa tttctttata cttttctccg attataaatt cctaacccct tgagaaaatt
     8701 acgcatatat ttatctccgc aaggcttaaa atttcgttgg ccttcttttc tcaaaaatgc
     8761 cccgatttcg ccttttgatg gatagatttc tgcctcagca aaaacgtcta aaatatcatc
     8821 agtggtcata gacaaggcga tttttaattt ttttagcaac acattgttga tatatttgat
     8881 ttccttgctt ttttgcggcg tgtctactat cggcttatct ttttgcggac cacgcgccaa
     8941 gagaatcagg ccatttagga atgcgtcaaa aacttggttg ttgatggttt tttcatacac
     9001 attattttct gcattatctt tgttcttatc ctgtactttg gtcagtaccg cctgagcttc
     9061 ttctttagtc agtggtacac cgcctagctc aaaagcttta atcaaatccg tatctttcaa
     9121 atcaatcgca taacgcaatc gcaataacct atcattgtta ttcatttgtt cctccgaagt
     9181 atttagtctt tactttctta cgtacttttt gtcttgctaa gatttttagg atcaatgata
     9241 aaaaccgcat cacttatgat aaggcgcccc cgcattgatc cggaaggcac ggtagatttg
     9301 ctcgacaagg atcagacgca tcaattgatg tggatacgtc atttttccaa aagagatctg
     9361 agcattgctt cttttcatga cggcttcgct gagtcctaaa gagccgccga taacaaaaac
     9421 aaggtggctt tttccttgga tacctaattg gtcgatttgt ttagcaaaag attcacttgt
     9481 aggatttttc ccttcaattg ctaaggcata gacgtattct tgttctttga ttttcgctaa
     9541 gatccgttcc ccttcttttt cttttacttg tctcatttgc gcttcgctta aattctccgg
     9601 ggctttttcg tcaggaactt cgatcaattc gatttttgca taggcattca gccgtttgac
     9661 atactcattg ataccttgga tcaggtattt ttcttttaat tttcctactg agataatttt
     9721 gatattcatg ttttctctcc tttggtcttc atgctcactt tgtttattat aatcaatttt
     9781 ttcgtctata tgtttatttt ggctattttt gttaaaaaga tttccccaac agttatgcac
     9841 aacatccaca ggtttatcca cagaaaacag tgttatttta ccttatttca tcacattcat
     9901 taggcaaaca tacgttttat aagaatttat ccactttata cacaggctgt ggatatgttt
     9961 tatcccttat ttttccaatc ctatccgcgt agttttacac agtatctgtg gaaaaagaaa
    10021 atcaaatatt ttcatctgaa aacaatgatt tttcctaaag tttttcataa aaagttcaca
    10081 gtctcaaagt agactaaagt caacaaataa gactatacta acagtagata aatgaatgta
    10141 attggaggag gaacacatgg atagaaaaaa tgtgacacca aagatgaaaa aaagtaaaaa
    10201 taatagcctc tggcgtaagt taggtctggg gcttgttgga gggatcgttg gaggactact
    10261 gacagctggc attttttatg cagcgatggg cactggcaac aatagttcta ctgctacaag
    10321 cggcaatcag aattctgccg gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc
    10381 tgatatcacc aaagcggtag ataaagttca agacgctgtc gtttctgtta tcaacttaca
    10441 aagtcaaaac caatcaagcg gttttggcgg actattcggg cagcaagaag aaagtagcag
    10501 ctcagatgat agtaatttgg aagcttatag tgaaggaagc ggcgtgatct acaaaaaaga
    10561 cgggaatact gcttatgtag tcacgaataa tcacgtagta gatggccagc aaggtcttga
    10621 agtcttgatg aaagacggta cgaaggtaaa agctgaatta gttggaacag atgcttattc
    10681 agatttagcg gtgttgaaga tcaattcaga caaagtcgaa acagttgcct cttttggtga
    10741 ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt ggttccccac taggatctga
    10801 atatgcaaat tctgtcactt caggaatcat ttcttccttg aaccgacaag tgacaagtac
    10861 gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa acagatgcag caatcaaccc
    10921 tggaaattct ggtggtcctt tagttaatat cgaaggacaa gttatcggta tcaactcaag
    10981 taagatcgca agtacttctg aatctacttc taatgtaagc gtagaaggaa tgggatttgc
    11041 tattccaagt aacgatgtag tgaatatcat caatcaactg gaaaaagacg gaaaagtgac
    11101 acgtccagct ctaggtatca caatggtcga cttatctgcc gtttctactc agcaacaaga
    11161 acaaatcttg aagattcctt catctgtaac taacggtgtc atcgtgacaa atgtccaaac
    11221 agctacaccg gctgaaaaag ctggattgaa acaatacgac gtcattacta agatcgatga
    11281 caaagaagtt tcttctggtg tagaacttca atctgctttg tataagaaaa aagtgggaga
    11341 ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa acaactactg ttgaattgac
    11401 gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa aattaataaa aaagaggttg
    11461 tgacaaagac ttgtcacaac ctctttattt tggaaatgct tctccgacta cggcatcctc
    11521 aggcaatact aagattcctt tttttgctgg agcatttggc aaatgcaatt cacgagcgga
    11581 acagatcatt ccatagctat ctacccctcg caaagttcct ggccagatca tcattccatc
    11641 tggcatcata gctcctggtt ttgcaacgac aactttttgt ccagcttcga tattgggtgc
    11701 accgcaaacg atttgcaata ctgtaccatt gtctacttct gtctcagtaa tcgataaatg
    11761 gtcagaatca ggatgtggga cgcatgtttt tacgtacccg acaacgatct tcggttcatg
    11821 atcagcttca agctgttctt cgaatcctgc ttctttgatc aatcgattca atttcgctaa
    11881 ttcctcatct gttaagctta cttggccgtt cccttcgatt gttagatgat cggagatatg
    11941 gaaaaagttc caagcaacaa cttgaccttg ttcatctttg atccgagcaa cattccattt
    12001 gcgttcaaca gtgttttctg ttcctttatc gtcggctata ataaccatca aggtgtctcc
    12061 gacgtgtgct ttgttataag caaaaatcat gttctttcct ccattatcct ttgctgagat
    12121 ttatcttaac aaaaaattgc attcactgca tgaaattttt ttcatgcttt atttccgaag
    12181 aagcctcttc tggaacacca tttattcgta acaagggact gtgacaagac ttttgtcaca
    12241 gtccctttac tgttttagtt aattgattaa aaaggtcgta caagttccac ttgcttaatt
    12301 acttggctgt tcttcttcgt cagaaacgat caagacatca caaggtgaat gacgtatcac
    12361 gtagctggct acacttcctg tcataaagcg ttcgactgca tttagacctg actgcccaac
    12421 catgattaga tcgatctcat atttttttgg caatttgact gccatggatt cacgaacaga
    12481 accatatgca ataattcctt ctatatttgt gtaatcaaca gatttcccat attctttaca
    12541 ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg actagatttt gatcaatagg
    12601 tgcatagccc ataaagttat aaaattgctg atcgattacg tttgctacat atactttccc
    12661 attattacgg cgagccactt ctaccgcttt tttgaaagca gcaagtgctt gttcactacc
    12721 gtcgatacct actaaaatat tcttatattc ttgattcatc ttgacccctc ctaacagatg
    12781 tcagcctttt gttacacttt ctaggaacga ttcgatttcc tgttttgttt tacgatcttt
    12841 attcactaga cgacctaatt cttttccgtc ttggatcacg acaaaacttg ggataccaaa
    12901 aatgttccat tcagcggcta catctatgta cttgtcacgg tctacttcaa taaattgcca
    12961 atcagaaaag tcattttcaa tttcaggcat ttgcggtttg ataaaacggc agtctccgca
    13021 ccagccggca gtaaagaaaa atacactctt tccagtttct acgtagcctg ctaattcttc
    13081 taaactattt gggataatca ttctttgtcc tctactttct gtttatgtat ttttcaatta
    13141 aattatacac ctttctttcg aactaacaaa cattgatcct ctttttataa aaaaaatctg
    13201 gcaaaaagca atcttttgcc agattttata gtttcactat cgcattattt tccgtatacg
    13261 atcgtattta ctgttgattt atccaaacct ttcagtgttt gaatcaacat ttctcgtgca
    13321 gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg tcccactacg
    13381 ccaatcactg tacttgggat tccttcgttt tgtgtatgag cagcacctgc atctgtaccg
    13441 ccttttgaaa caaagtattg ataagggata ttgtttgttt cagcgatatc cagcaagtac
    13501 tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa tgtaccttct
    13561 cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat ctgctgcaga gcagtctact
    13621 gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg caagcctact
    13681 tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt ttgcaacgct
    13741 tctaaagctt ctaataccat tgtacagcca tagcggttgt cccatgattt actgataata
    13801 ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc tcctggaagg
    13861 acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa aatatcggtt
    13921 acttctactg atttttgtcc gcttgttcct cgcaataaat gtgggggaac ggaagaagaa
    13981 atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgctgagc agaaacaacg
    14041 taaggattcc agccgcctag aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc
    14101 agcatgaatc cgacttcatc catatgtgct gcgaccatta cacggggtgc atccgcttct
    14161 tcatgatggc gcagaccaaa aatgccgccc aaaccatcta actgtaactc atctaccagc
    14221 ggttccatat gactttccat ataagcgcga acatcttgtt caaaaccgct tgttccttgt
    14281 aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa
    14341 tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg attttttctg
    14401 cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta tttcaaagaa
    14461 aatatgagag tgtggtaaaa taagcacaag gagtaacaag tgagaagaag gaggtatgtc
    14521 cacatgtatg aagaaagcga actaagctat tttaaaggcg ggttagcagt tggaatcggt
    14581 ctaggattat tgagcggggt agcttcaacg atttggtatc atcgcaaaaa agtattgtca
    14641 gcagaccaag tacttgaaaa tatcaagtca tcttttctaa aagaaggtgc aatcgaaggt
    14701 tcttggatct cttttgaaaa ggaacctatt aggaaattcg ccgttcggtc gagtgcttat
    14761 cggggaggtc tttctcgaat agaagatggc gaaactgttt attatgaatt tttagcagat
    14821 gctcatacag gaaccgtatt agaaatttca agaaaaaaag gtgcaacaga ttaatacgtc
    14881 aaaaaagtga gagaacgaat acgcacccac tattcaaaag agaggtatca tgtccttctt
    14941 ttgtagtggg tggtttttta gatagagctt tccaaacata tattccagta ttttcttttt
    15001 aaataaatga tatttagcgg gaaaaactag catttccttt gattatgagg tatttttaat
    15061 ttagcttttg ttttctatag ttaaggagag tgaaaaatgg aacaacaaga aaaaattgct
    15121 gttctgattc cttgttacaa tgaggaagca acaatttcaa cagtcattgc agactttaaa
    15181 cgagaattac cagaagcaga tatttatgtc tatgataata attcgaccga tcgaacatac
    15241 gaattggccg ttcaaggtgg agcaatcgta aaaaaagaac ctcgtcaagg aaaaggaaat
    15301 gtgatccgcc aaatgttctt tgatatcgat gccgactatt atttaatggt agatggcgat
    15361 gatacttatc ctgcagaagc tgtacatggt ttgctcgaga aattacgttc tggtgaagca
    15421 gatatggtca ttggtgatcg cctatcaaat ggaacctact ttgatgagaa taagagacct
    15481 ttccacgatt ttggaaataa ccttgttaga aatacaatta ctagaatgta taaaacaaaa
    15541 attcttgatg tcatgaccgg ctatcgagga ttcaatcgta tctttgtcaa aagttttcct
    15601 attatgagtt ctggttttca aatcgaaacg gaattgacga tccatgcttt agataaaaaa
    15661 ttcaagttcg ttgaaatccc aatcgattat cgtgatcgtc ctgaaggcag cgaatcaaaa
    15721 ttgaatacat tctctgacgg attcaaagtg atcatgatga ttattaaaat gtttaaagat
    15781 tataagcctt tgcttttctt tgggatttgg accatcttct tctttttatt tggattggtc
    15841 acggggattc ctgtgattcg cgaatatatg ctgacaagct ttattacacg catcccttct
    15901 gcgattttaa gtactggatt gatgacttta gcacttcttt cattagtaac agggttgatt
    15961 ttggatacag tcgtcacgaa cgctaagaaa gaatatgaat tgaatctata tcatgtttat
    16021 catgaatacc acagtagaaa gtaaaaaaag gatcaagact gcaaaaataa tttgcaattc
    16081 ttgatcctct ttttaattaa ttatttcgcc aaacgataga tggcatctgc atagattgct
    16141 gttgctctga acaagtcatc aagactcata aattcgtttg cttgatgcat tgtatcagta
    16201 tatcctggga acatcgctcc ataagcaacg ccacgtttca acaaacgtcc gtaagttccg
    16261 ccaccgatga tttgttcgta gcctttttct ccagtgtggt cttcatagac ttgcaataat
    16321 gtagccacca atggatcatc cattggtacg taatgtggtt ccattacgcg agcacctcga
    16381 gtaactgttg cgccttcttg ccccactgtt tgttcgatgt cactttgcaa tccgtctact
    16441 gttacacctt ttggaaaacg gaaattcata ttgatgaagt tcgcttcctc tgtaccattt
    16501 tcttcaaaag caaatagtcc agcattcatt gtcaatttgc ccattttttc atcttcataa
    16561 gcgactccta gtttttctcc ataaaaatct tcatggacat aagcagctgc tacatggata
    16621 aattgtttag cagatcctag gaattcatag ttatctaaga acaatgccaa gaaagaacct
    16681 gcattgatac cagattgtgg gcttgcacca tgtgcgcctt taccaactaa ttcgattttt
    16741 acatacgtat tgtccgcttc aatcgttccg cttactggat ttgcttcgat gaattggtaa
    16801 aatgcttctt ccatcgcgat agctgcatct gcacttggta cttgaagtgc tgcagttgct
    16861 gttcctggca ccatattttc gcgaagtccg gaaacaaaag atttcaagac ataatctcct
    16921 gcattgtcgc ctttgaagcg taaagctaaa gaaacatttc ctttttcacc attgatgatt
    16981 gggaattcag catctggtga gaaaccaaaa tctggttctt cttcatgttc gaaataataa
    17041 gccatatcgc cccagccgct ttcttcatcg ctgccgacta cgaagcgaac tttttttgat
    17101 agtttcaagt caagttcttt aatgatcttc atcgcatagt aagctgccat acttggacct
    17161 ttgtcatcgc ttgatccgcg tgcaaagatc tttccatcct tgattactgg ttcataagga
    17221 tctgtgtccc agccatctcc tgcaggaacg acatccatgt gtccaaatat ccctagcgtt
    17281 tcgtctcctt cgccaagatc aatatgccct gcatagttat cgacattttt tactttaaat
    17341 ccatcacgtt cgccataagc taacatatgt ttcaatgcgt cgcgaggtcc aggtccaaat
    17401 ggtgcatctg gtgttacttt tgagtcatcg cgctcgctgt tgacacgtaa aagatttttc
    17461 aagtcttcaa gcaagtcttc tttacgtgct tctacttctt tttgccaatc aattgtcata
    17521 aagttaatcc tccacttatt taagtcttcg tgttcatgat accacaaatt acagaaaaaa
    17581 atagttcaaa aaatctgaca attccttttc ttatctgttt ttctctaaaa aaaatatacg
    17641 ctataatgag taaaaggagg tttttagatg ctgataaacg aacaactagt gaaaagagtc
    17701 atcacacctc gtccaaaaat ttcacataaa ggaacatttg gacgtatcgt tttgattggc
    17761 ggcaatgaac aatatggcgg agcaatcatt atgagtgctt tagctgccgt acacagcgga
    17821 gcgggtctca cgactgtagt aactgatgaa aaaaatcgga gtgctcttca cagtcatctg
    17881 ccagaagcaa tgattgtagt ttggacggat actgaccagt ttctttccgt aataaagcaa
    17941 gcagatgtac tcttgattgg tcccggattg ggtacttctt ctaagagttt ggaacgtttc
    18001 aaacaagtac tagaaaacca atcaaaagaa caatggctgg tgattgatgg ctctgcttta
    18061 agtttacttg ctgctaatca tcttttgcta ccctatcccg aacaaactgt acttacacct
    18121 catcaaatgg agtggcaacg gttgagttct ctaccgatac aagaacaacg ggataaagaa
    18181 aacaaaaaga agcaacagga aatcggtgct gtgctcgttt tgaaaagtca tgagacaaaa
    18241 atttatggtc accagcactt ctacaccaat cctttaggtt ctgctgctat ggcaacgggc
    18301 ggcatgggtg acactttagc tgggatgatc acaggatttt tagcccagtt tcctcaaaat
    18361 gaccaaacga ttgcagcagc tatttatctt catagtttca ttggagacca gctggcaaaa
    18421 gaacaatata ttgttttgcc gacacaaatc agcgaagcac ttccgaaatg gatgaattat
    18481 ttttgtaaaa aatcaatgga aaacgaggca tgagtcatgg agaaatttac aggttatgtg
    18541 cgagaacctt tttattatga aacagatcaa atggggatta ttcatcacag caattatatc
    18601 cgctggtttg aagaggctcg ggtagcctta ttggtacatc tagggtttcc ctataaaaaa
    18661 atcgaagaat ctgggatcat catccccgtt ctggaagtaa attgcagcta taaagaaatg
    18721 atccattacg gagaaaaagt gagcatccat ccaactatcc aaaagtatac ggggacacgg
    18781 ttggatttta gctacgaaat tgtgggaact gaagacaaaa aattaaaaac tactggcagt
    18841 agtaaacact gttttttact ttctgattcg caacgattag tgaagctgag caaagtcaat
    18901 ccggagttag atcagttatt ccaagcgtac gcttctttgg atgatgtaaa ttcttaataa
    18961 agaccgtcta agcatttttc ttgtattgtg tcgagtagtt cgtataatga aaagaaaaaa
    19021 gcaggtgatc cccatgtatg tcgttaaagt aatgcacggt tacatcgata aaactggctg
    19081 tcgcactcgg gaaaaaaact tagacaatct gctcattttc aaagacaaaa aagaatccga
    19141 agcttttgct aaaagaatcg gcggtcgtgt caaaccgatc caagaggtac gaccagattg
    19201 attcaatatt tttcttttcg aggctctaat aggcatgcct atcagggctt tttttgcaga
    19261 ctgtgttttt atgcccacac gatttgtaag cgatttatgt tataatagat tatatctgca
    19321 tagaaaggaa ggttaaatgg ttatcaataa tttgcaacag caagcttatg aatctatacg
    19381 taaacaaatc atttattccg agttagcacc tggaagtaaa atctccgata aagtgttaga
    19441 agaacaacta gctatcaggc gtacaccgat tcgagaagca ctgatccaac tgcgcaatca
    19501 agagcttgta aaaaccattc cacagtccgg cacttatgtt tcaaaaatcg atatccgatc
    19561 tgcttcatta gcccgttata cgcgggaaaa gttggaaaaa ccgatcctgc aagaatgcag
    19621 tgcgaaaatg accaagcaag atcaagctaa actagaaaaa atactagaac aaaccgatca
    19681 ggcagtaatc gctcaaaata aaaaagcttt ttttgatttg gatactgatt ttcaccgaac
    19741 ttgttatgaa attgctggaa aacgagaaat ttgggattgg ctagaaagtt atagtacgca
    19801 cttgaaccgt ttcagatggt tacgtttgac gatttcagaa ttagactggg gtcgagtatt
    19861 agatgaacat cagacaatgc ttcaatcaat gattgatcat aactttgatg aagtcggatt
    19921 tctttgcacg atgcatttac atatgattat cgaggagcaa gaatacgtga ttcataatta
    19981 ccccgattat tttgaagata ttcaggacag acccatcaaa taagaggttt tggaatcagc
    20041 gttttgacct gagtattaga gaaagaaacg gcaaaatagt tcctcatatt ttgccgtttt
    20101 ttttgagcta ataccgaggg tcagctgatt gcacaccgtt tattgagagg ttgtgaaaaa
    20161 gctgccctgc atcaagtaat aagaacagcc aagtaaaaat agcttcttat gtttttaact
    20221 tggctggact tattaccgca gatgcaagct tttgaacacc gttttccaag aggtcgtgag
    20281 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt
    20341 gaatattttg acttgtgacc gaggagttga caggagaaca ccgtttattc ggaaaaaaag
    20401 gactgtgaca cgacttttgt cacagtctct ttatttgtaa tctaaatcat actccgtaca
    20461 tcttttttct atttttttta tttcttctaa gatcccttgg ctacgaattt cattatatgg
    20521 ccaaagtaag actgcctcta atacataagg cgaaaaatcg ctttgattca tctcttttga
    20581 aaaactcatc gctgctatct cgctcaatgc acgaatgttt gattgatgtg tataaaaagg
    20641 caaccgccac agcacaattt tttcgctttg cgtataaatt cccttaacag aagcttcgat
    20701 gtggtgaaat agttcatgtc caataatcaa agcttgcgct gtttctttag agatcgaagc
    20761 tagtcctgaa cattttaatt tttctaacgg ctccgtcatc aatcgaatcc cttcatcttc
    20821 tgcataagtt gcaaaagtga ttcgttcgtt tatttgctct gattcacagg aaacgattgg
    20881 aacagccatt tgttcacaaa tttctcctac atcggcaaaa gggtactttt ttttgatgct
    20941 acatgccgtt tcgataccac aatcgattga ttgttgtata agaattttct gcttggacaa
    21001 aggaatcttt ttccaaaata aatctcgccg aaactggtag aatccccaaa tttcttcggg
    21061 gatctgttgt aattcgttta gtaactcttc taacttatgt ttgtttgata tttccggcaa
    21121 cgacgatcac ttcttcctcc ggttccatag tagatacttc cagatccatc aaaagctgta
    21181 gttgttccag atccgtcgct gtaaaatcag agactcttgc tcctaagcag agataaaatt
    21241 ctggtcgggc aatcaattca gtttgataaa ctgccatatc ttcccaaagt tgttcgacag
    21301 ctgtcatata attggctgca gttgttttca tacatgaagc atgtccacgt tggaccttga
    21361 taaatccttt ttgatcgatg atccgaagac ttccagcatt ttttgaacga ttctgttcgc
    21421 cgcatacata aaagaaaggt gtattttcca ataagcaaac ttcattttta tttaatcgca
    21481 tatcctctgc agcaagctct agtgcttctt ctgttgtgat ctccttggtt agatcggttg
    21541 ctttcacttc cgtagaacca gtagcgatag ccgttacttt tgaagtctgt ggatcgattt
    21601 ctacgtggac ttcgatcgat tctggtgtag cgcctgattc aatcgcttta ttcatcgctt
    21661 catttttcaa cgatcgtata tcttctttac ttggcgaagg gatgatacgt tctacaacat
    21721 ctcggaccat tgcaagtgct acaccaatag aagaaatcac ttcggcgttt tcaggaatcg
    21781 aatatttcac acccatttta ttactaaagt aagtaatcaa tgaggcagcg ccgcccccta
    21841 cacctacaag agaaatctga tctttctcca agtgatactt gtctgccaat tctaaaatca
    21901 ccggttcaat cttagcatag gatttttcca tgatctgttc tgcgatgtct tcaaccgtcg
    21961 tatgacaata atcagctaat gcttggatgg cttttcgtgc agatgggaca tttccataag
    22021 aaaaatgttc ttcttgcact aatccaagga cattggctgc acatgtattt gtaatagtca
    22081 cttcttctcc gtcttccatc actaccttga cgtaatcagc agggtctcct tctttaggag
    22141 agaaaaattc aaccttagga cctttgattt tttctgtctc cgtaaatact gcataatcta
    22201 gaccggcaat atgtgcagaa cgtggaccga catcgatgat tcctgactga ttagcccgta
    22261 ccatcgaacc gcctgccaca cctagaaccc gaacatctaa tgaagagata tatgttgggt
    22321 ggccacctac aattgaataa tcgattgctg gacgtccatt tttaatgacc ccgatattgg
    22381 ttgttgttcc gcctacttca aaataaacac cattagacgc tctcaagtac atcaaagagc
    22441 ccatgactga tgctgctgga ccggaaagca tggttaaaac aggtcgtttt ttcatttcat
    22501 tgatttccat tactccgccg tctccgcgca taatcatcaa agaaacattg acccctgctt
    22561 ctcgtacgga atcttctgta gaggttgccg tatctagcat ttttggtaaa atagatgcat
    22621 tgattgctgc tgtccgagtt cttcttgtca atccatataa ttttgtgatg tcagaagcca
    22681 tcgttgtagg gatcgatttt accgatgctg cttcataaac aactcgttct ggttccccat
    22741 tatctactcc aaatgccata gaagaaacca agacctccgc tctttctctt tctaaagaag
    22801 aaatcgtttc tgaaacacgg tctacattta agtgtttgac aggtaagaat gcattgacaa
    22861 tctctatttt tttcttattc cctaaatcga tatcgttcaa tctagtttgt cgttttgcta
    22921 aaaaaccttc taatccacct tttgccatcc cgattacgcc gacttttgct acgtctcctt
    22981 caatcagtgc attagtcgct tgtgtggtag aatgtgcaac aaacacaaca tcttctggac
    23041 tgatattatt ttccagcaaa caattttgaa aagactgaac gacacctgcg gctactccac
    23101 ggacatcatc gtgggttgtt ttcactgaag attttccgat gatttcgtgc gtcgcattat
    23161 cgattgcgac tgctttcgta tgtgtaccgc caacatcaat tcccattcgc acaaaacgtt
    23221 tcattttctt ttcctccatt catctaatcg tttttatcga cgttttgttt actaggcgtt
    23281 cccgtacaat aagaacgtga tcaggcataa gatcgctccg atgatccaac cagttgggat
    23341 ggatttcttc atatattctc gagaagttac ttttgtataa cctaatcccc aagcaaccca
    23401 agattgtgta atatctaaat gctgaggagc aatcgttgtg attgcaaata acgggaacaa
    23461 aaaggctggc gacatcgttg gattaacagc taatacgaca gctaaaatag ctgagcctga
    23521 accgactaga ttcattggac cgcggaaaaa tcctagtggt gtcaatatag caaatacgat
    23581 tgccaagact aatggtgatt gcggtatcca atctccaaaa atagctgaaa aataaggaga
    23641 agcatacgca gctgaattat tgaacatcgc taaggtcatc aagaaaccaa ccattggcgc
    23701 aacatctact gcgccatctg taaataattt cgcaaactga cgacagattt ctttataacc
    23761 tccgcgcaat tttccagtaa acagcaatgc ccagatacct gctagaataa aacctaagat
    23821 gattggaaga tctaaaagaa cgacccctaa aactggtaat aatacggcta accatgagat
    23881 cattggtgcg tcgccacctt cagctggtac gtttgctgcc attgcatagc gtttcttttt
    23941 gttcattgat atattagcga ccgttagtac cacaactaag cttacaatca tcccaatcat
    24001 accaatttgg aaatagtcat tataagtata gctttcagcc gctggattga aaccagcata
    24061 aatcgtttga tattgtttga aattgacgat attagcaaag attccagcca taatcgagcc
    24121 cataaatgag aacagtgcaa ccggtactga aatacctaat gacatcaaga ttggcaacac
    24181 aataactgca atagaaatca ctggtccgat tcctgtcatc gacatgaata taacagcggt
    24241 tacaatacat agtaagctca tcgtgattct tggcttgtcc cctccaagtt caactacctt
    24301 tcgaatcagg gtagctgcga ttccgctatc tactagaaca cgcccgaaaa aggcaccaaa
    24361 gaagacattt accaagatcg atttggcgta ctcagctggc cccgtcgtat agatatgcgt
    24421 caacacatca attactcctt gcccttccat cgccgggttt ggtgaaaacg tgtttccgac
    24481 tagcgctaat cccatccata cagtagccat gatggcaaag ccgaccatca agttgtagcc
    24541 ttttacacag taccagacca tgaaaaagaa tgagataact aacaaaatac caataataat
    24601 atcactcatg ttattcctcc gtccattttg tttttctgat aagcaaacta atatatcagt
    24661 caaactcatt ctaacttatg tagttagcgc tttcattaaa gggtgaccat aaatgttcag
    24721 ttttcaatta aaaaacctta attatgctat tttagactta tacgttattg tcctgtttca
    24781 tttgatggag gattgtaaat aaaaaaagag gaaagaacat aacttttcgt tatcttcttt
    24841 cctctttata attgtttagc ttatttagcc aaagcttctt ttgcttggtc agccaatgca
    24901 gtaaatgctg ctgcatcgtt aacagccaag tcagctaaca ttttacggtt gatgtcgatt
    24961 tcagcaactt tcaaaccgtg cattaatttt gagtagctca agccattcat acgagctgct
    25021 gcgttgatac gagcaatcca caatttacgg aagtcgcgtt tcttttgacg acgatcgcga
    25081 tatgcgtagt tgtatgaatt cattacttgt tcttttgctg ttttaaataa agtgtgtttt
    25141 gaaccgtagt aaccttttgc taattttagc actcttttac gacgtttacg agttactgtt
    25201 ccacctttaa cacgtgccat gtataattcc tcctaataaa aataaatctt cagattctgt
    25261 tatttgtact tcttatttca tttttgctag ttgttggcga atacgtttga agtcgccact
    25321 agaaaccatg cttgctttac gcaattgacg gcgttgtttt tttgttttgc cgtggaaacg
    25381 gtgacttgta aacgcgcgga agcgtttcaa tccgccttta ccagtacgtt tgaaacgttt
    25441 tgctgatccg cggtgtgttt tttgttttgg catgactata tttcctcctc aaaatctttc
    25501 gttatccgac tatcggctgt ccaacagcca gttagtcatt caaattactt actgtcgttt
    25561 ttcggtgcca gcgtcaagaa catgctgcgt ccgtccattt tcgctttttg ttccactgtt
    25621 gcaatatccg cagtttcttc agctaagcga ttcaagactt tttgaccaat ttctttatgg
    25681 gtaatggcac ggcctttgaa acggatagaa gctttcactt tatcgccctt ttccaagaac
    25741 ttacgtgcat tacgaagttt tgtattgaag tcattcaagt cgatcgtagg acttaagcga
    25801 acttctttta cattgatcac tttttgtttt ttacgcgctt cgcggtcttt cttttgttgt
    25861 tcgaaacggt atttaccgta gtccatgatt cgcgcaactg gtggtttcgc accaggggca
    25921 actaacacaa gatccaagtt tgcttgttct gcaatattca atgcttctgc ttttgtctta
    25981 actcctaatt gctcaccatc tgaaccgatc aatcgtaact ctcgtgcacg aatgccgtcg
    26041 ttaaccatca tatcctttgc tatggtcatt cacctccaaa tattatgaga gaaagaaaag
    26101 cttaccgaat aaacggcaat aaaaaaacga gttctcttac agaacccgcg cgtacttcac
    26161 ctaatagaac cagtgaaaac tatctagccc agtgacatta tcaactaagg cgagaagcgg
    26221 gtgcttctgc ttttatttct caaccatcat agtatagcaa aaactaatct ttttgtcaat
    26281 gtttttcata cagtttgtct tccttttctg aaaatgatta gattagcaga ctttctagca
    26341 gtgattcccg cttctttgct atactaaaga aaaacgaggt gaacccacat gaaaaaaatc
    26401 gaacctattc caccttctct tcctcttctt acaaagggag agttttattt agaagatgag
    26461 gagatcttct ctggtcttct gatcaacggg ggtgaccaaa gctatctcga tgccaaaaat
    26521 atggtattaa gagagagtca tatccaaaaa cttacgatgc aaaaaacagt tttacgtcat
    26581 tttgaatgca gtaatgtcat ttttgaaaaa tgtgattttt ccaatctgga gtggattggt
    26641 ggcagtttcc accaagtcgt gtttcatcaa tgtaaattaa caggtacgaa ttttgccgaa
    26701 agttatcttc gagactgtac tttcactgat tgtattgctt cgatggcttc ctttagtagt
    26761 acaaacttga aagctgtctc cttcgatcat tgtcaattag aagacagcga attttatgag
    26821 gtcacttgga aaaacctttt tttgtctgac aatcagttga ccggttcgaa ctggtttcga
    26881 acttctctga agggattaga ctttacgact aatttcttca cagcaatcgc tttatctcaa
    26941 gattctttgg cgggactaat cgtcaaccaa gagcaagcac ttgtgatcgc ccaagccctt
    27001 ggtttgcaga tcaaagacta gatcaacgaa tccgccgcca cagtgcaata agcatccaaa
    27061 tagataagac caaactgatg atcaaggcga ttccccatcc gaaaacggaa tgggtaaagg
    27121 gtaactggag attcattccg aagaatcctg tcacaatagt tggaacggtc aagatcagcg
    27181 accagaccgt caaaaatttc atcgtgtcat tcaagttgtt attcagcaaa ttattataag
    27241 tgccagagag ctggtctaaa atctgcgaag ctaagttcgt catttcaact gcttgtcgtg
    27301 cttcgatcag cgcatcgtcc aactcttctt tttccttttc tgacaacttg cgataaatag
    27361 ccaatgcctt gatttgttct aaaagtacta cattttgttt tgttcctgta actaagtaaa
    27421 ccaaaccgat ctctaaatct gaaagttgca gcagattctt gttcgttgtt ttttctctta
    27481 atttcagatt aagccgctga cgttcactat tcacttcctc gattaaagga aaaaaggaat
    27541 cagaaatcaa aaacaatgta cgaaacaata aactgtatac actcatgtct ggcattcgtt
    27601 ccaaaagcga tcgaattagt cgaatcacgt actcggtatt cggcgtggta aatgtaaaca
    27661 aaccgtcttc ttttaaaata aaagcgatag gactagtttc ataatgatta tcgacttttt
    27721 gctggtgcgg aacatttgat acaagcagta acgcttcatc aaaagaatcg tactctacgc
    27781 gcgcccgttc atttttatct agagaataag tcagcatctc gtcactaatg ccatattctt
    27841 cttttaagtt tttaacagct agcacatcgt tttgctgtac attgatccaa aaattacggt
    27901 cttgattaaa atagtgtttt tcccgcatac tctatcctcc tgacatttgt ttccagtata
    27961 aagaaaaaag tcgctcaaat aaaggtttac cctttatttg agcaactgta aatcaaatgg
    28021 ctactttggt ttggcgaatc tctgctaatt tttcttcgat cattttcaag acttcttctt
    28081 ttgcttgagg atcttccaca aaattatagc ggtctccatc gatttggatt tttggacttt
    28141 ctttgtattc ttcataccat tgatcgtaac gtctgttcaa ttctttgtaa taatcgtata
    28201 gacttggatc aaaggacaat tgttcatacg aacgaccacg tttttcgata cgctccaaca
    28261 tcgtatcaaa agatacacga atatgaacta ataaatccgg atgtttttta tgggcagcat
    28321 agggaagttc ttccatcatg ttttccagca gttcatcata cacacgaact tctgtttctg
    28381 ttgctcttcc tagatctgca tttagatgga aaagcagtga atcttcataa atcgaacggt
    28441 ccaatacatt attgtcatct ttcattgctt gttttatgct tgcaaaacgt ttattcaaaa
    28501 aataaatttg cagcaaaaaa gcatactgtt cagggtttgc ataaaataag ggcaagactt
    28561 cattatcatc aatcgattca taaaatgcct gactatcaaa gtgttcagcc atcatttctg
    28621 ttaaacttga ttttccggca ccaattgtac ctgctaaaac gatcacgctc atttacctcc
    28681 agctcacttc atcactaaat attgtgttta actccaacct ttttaaatat acccccaata
    28741 tctagtagtg tcaattgtat ttttttctag tcaaagaaga aaaaaagtca aaaaattctt
    28801 aagaaaaaag tgttttttct ttagaaaacg tattcttagt tttacttctg agatggtttt
    28861 agtaaaataa aggaggaatg ttttttgaga cccttagtgg acaaaaaaat tcgtgaagca
    28921 gcgcaactcg ttttgggcgc tttatttttt gcttaaaaat cacttttacg tgagaaggag
    28981 ttagattatg gcaaactatg gccaaggaga atcaagcgga aagatcatat tgatgggcga
    29041 gcacgcggtt gtttatggag aaccagcgat tgcctttcct ttctatgcaa caaaagtcac
    29101 cgcattcctt gaagagctgg atgcaatgga cgatcaactg gtttcttcct actattcagg
    29161 aaatttagcc gaagctcctc atgcattaaa aaatatcaaa aaattattca ttcacttaaa
    29221 aaaacagcat gacatccaaa aaaacttgca actgaccatt gaaagcacga ttcctgctga
    29281 acgtggaatg ggatcaagcg ctgcagtcgc cacagcagtc actcgtgctt tttatgatta
    29341 cttagcattt cctttgtctc gtgaaatact attagaaaat gtccagcttt cggaaaaaat
    29401 cgcccacggt aatcctagtg gaatcgatgc agccgctact agcagcttgc agccgattta
    29461 ttttacaaaa gggcatcctt tcgactactt ttctttgaac atcgatgctt ttttgattgt
    29521 cgctgataca ggaatcaaag gacaaacaag agaagccgtc aaagatgtcg ctcacctctt
    29581 tgaaacccag cctcatgaaa ctggacaaat gatccaaaaa ttaggatact tgacgaagca
    29641 agcaaaacaa gcaatcattg aaaattcacc agaaacgttg gcacagacaa tggatgaatc
    29701 acaatcactt ctggaaaagc tgacaatcag caatgatttt cttaatctat tgatccaaac
    29761 agcaaaagat accggggcat tgggtgccaa attaactggc ggtggacgcg gtggctgtat
    29821 gattgcatta gcacaaacaa aaacaaaagc ccaagaaatc agtcaagcac ttgaagatgc
    29881 gggtgctgct gaaacttgga tacaaggatt aggagtacat acctatgttt aaaggcaaag
    29941 cacgcgcata taccaacatt gctctaatca aatattgggg taagaaaaat gaagaactca
    30001 tccttccaat gaacaacagc ctttcgttaa cattggatgc attttataca gagacagaag
    30061 tcatcttttc tgatagctat atggtagatg aattttatct agatggcacc ttgcaagacg
    30121 aaaaagcaac aaaaaaagtc agtcagtttc ttgacctttt tcgtaaagaa gccggcctct
    30181 cactaaaagc ttcagtaatc agtcagaatt tcgttcctac cgcagctgga ttagcctcct
    30241 ctgccagcgg gctagctgct ttagcaggcg cttgcaatac tgctcttaag cttggattag
    30301 atgatctctc tctttcaaga tttgctcgac gcgggtctgg ttcagcttgc cgaagtattt
    30361 tcggtggttt cgtcgaatgg gaaaaaggcc atgacgactt aagttcttac gctaagccag
    30421 tcccttccga ttctttcgaa gacgatttag caatggtttt cgttttgatc aacgaccaga
    30481 aaaaagaagt gtccagcaga aatgggatgc gtcggacagt cgaaacatcc agtttttatc
    30541 aaggctggtt agattccgtt gaaggggatc tatatcaatt gaaacaagca atcaaaacaa
    30601 aagatttcca acttctcgga gaaacgatgg aaagaaacgg actaaaaatg catggaacga
    30661 ctttagctgc ccagcctcca ttcacttact ggtctcctga cagcttaaaa gcgatggatg
    30721 cggtgcgaca attgagaaag caaggcattc catgctactt tacgatggat gctggcccta
    30781 atgtcaaagt tttagtggaa aacagccatt tatctgaggt acaagaaaca tttaccaaat
    30841 tatttagcaa agaacaagta attactgcac atgctggtcc aggaattgcg attattgaat
    30901 aaagggagat ttttatgatt gaagtatctg caccaggcaa actctatatt gccggagaat
    30961 atgcagttgt tgaaacaggc catccagcag ttatcgctgc agtcgatcaa ttcgtgacag
    31021 tcactgtaga atctgcacga aaagtcggaa gtatccaatc tgcacaatat agtgggatgc
    31081 ctgtacgttg gacaagacgc aacggagaat tagttttaga tattcgggaa aatccttttc
    31141 attatatcct tgctgctatt cgcttgactg aaaagtatgc acaagaaaaa aacattcttt
    31201 tatcctttta tgatctgaaa gtaacaagtg agttagacag ttcaaatggc cggaaatatg
    31261 gattgggttc tagcggtgcc gtgaccgttg caacggtcaa agccttgaat gttttttacg
    31321 cgttgaattt atctcagttg gagattttca agattgcagc actagccaat ttagcagttc
    31381 aagataatgg ttcctgcggc gacatcgctg ctagctgtta tggtggctgg atcgctttct
    31441 caaccttcga ccatccttgg ctccaagaac aagaaactca gcattctatc agtgagttac
    31501 ttgccctgga ttggccaggt ctatccattg agccattgat tgctcctgaa gatttacgtt
    31561 tattgattgg ttggacgggt agccctgcct ctacttctga tttggtcgat caagttcacc
    31621 gttcgagaga agataaaatg gtggcttatc agcttttctt aaaaaatagt acagaatgtg
    31681 tcaacgaaat gatcaaaggg tttaaagaaa ataatgtaac gttgatccaa cagatgattc
    31741 gaaaaaaccg acaattactg catgatttat ctgcaatcac tggggtcgtc atcgaaacgc
    31801 ctgctttgaa caaattgtgt aatttagctg aacagtatga aggagccgca aaatcttctg
    31861 gtgcaggtgg gggcgattgc ggaatcgtaa ttgttgacca gaaatctggc attcttcctt
    31921 taatgagtgc atgggaaaaa gcagaaatca ctccactgcc gttacatgtc tatagcgatc
    31981 aaagaaagga aaaccgatga atcgaaaaga cgaacatgtt tcactagcta aagcctttca
    32041 tgataaacaa aaaaacgaat ttgacttcgt acggatcatc cataaccctt tgccgcaaat
    32101 agcagtttct gatgtcgatc taagtacaca ggcagttggg tttacactaa gcagcccatt
    32161 ttatatcaat gcgatgactg gcggaagcga aaaaacaaaa aaaatcaacc aagatcttgc
    32221 aatcgtcgca agagaagcag atttgatgat tgccactggt tcagtcagcg ctgccttgaa
    32281 agtcccttct ttagccgata cttatacgat tatgcgacaa gaatatcctc acgggaaaat
    32341 cattgccaac atcggtgcag ggacttctgt cgaaagagcc aaagaagcta tccgattgtt
    32401 tcacgcagat gccttacaga ttcatttgaa tgcacctcaa gagttggtta tgcctgaggg
    32461 agaccgtgat tttaccaatt ggaaagtact tattcaagaa actcaaacag ccattgacgt
    32521 ccccctcatc gtaaaagaag tcggttttgg catgacaaga gaaacgctca atgatctagc
    32581 tgctttagga gttcatacag ttgatatcag cggtcgaagc ggaacaagct tcacccagat
    32641 cgaaaacgcc cgtcgctcca agcgggaact gagttattta gctgactggg ggcaatcgac
    32701 agtctcttca ttgcttgaag caaatgaagc agacacttct atggaaatcc tagcctccgg
    32761 aggtatccgc aatgcctatg acatatttaa agccctttgt ctcggcgcaa atgccgtggg
    32821 tacttcagga accgtactga cgcatctgat gaatcacggc gtagaagaaa ctattatact
    32881 gatgaagcaa tggcaagaag agcttcgtct gctttatact atggtcggcg caacgaatac
    32941 cgcggcccta caccaacaat ctctgatctt ttccggacca gtgaaggatt ggtgcgaagc
    33001 aagagggatc gacttagtga agtatggaag acgcacagaa aagagcgtgg gacaaaagta
    33061 aagaacactt ttgtctcacg ctctaaacac gtacaaacgg cggaagtaaa agcaactcct
    33121 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat tccttcttat
    33181 ttcttggagt taaatgcttc tgtcccagcc tcttttttcg aataaatgat gtttaatcag
    33241 ctgtccttcg gtattcgttc aatccgctga tttcacaacc tctccactaa acagtgtact
    33301 aaagctcttt taaggttgtt ttccgatata ggcaagtatg cctccgtcca catacaagac
    33361 atggccgtta acgaaatcag atgcgctaga agccaagaag acagatggtc cagccaaatc
    33421 gattggctcg ccccagcgtg ctgctggcgt acgtccaacg ataaattgat cgaatggatg
    33481 acgttcaccg ttttcttgtg tttcgcgtaa tggagcagtt tgcggtgtag caatataacc
    33541 tggtccaatt ccattacatt ggatattgta ttgaccgtat tcagaagcga tgtttttcgt
    33601 cagcattttt aatccgcctt ttgccgcagc ataagcactg accgtttcgc gtcctaattc
    33661 actcatcatg gagcagatat tgatgatttt accgccgcct ttttcgatca tatccggaat
    33721 aactgctttt gaaacgatga atggggcatt taaatccacg tcgattactt ggcggaagtc
    33781 tgccgcatcc atttcgatca tcggtgtccg tttgatgatc cctgcattgt tgactagaat
    33841 atcaactgaa cctacatctt ctttgatctg ttgaaccatc gcttgaaccg cttcttcatc
    33901 ggtcacatcg cagacataac cttttgcatc gatgcctgct tctttataat gcgctatacc
    33961 ttcatcaact gattcttgat tcaagttatt aaatacgatt tttgctcctg ctgtagctaa
    34021 agagcgtgca atctcaaatc caataccata gacagcaccg gtgactaatg ctacttttcc
    34081 atctaaacga aacatatcca tactaaattc catcattttc tctcccttat gcttatttta
    34141 attgatccat ttgcaccata tccatgtctg tataggtgat attttctccg cacattgccc
    34201 agatgaatga gtaattgctt gtacctacac ctgaatggat cgaccagctt ggcgaaataa
    34261 tggcttgttc attgtctaca actaaatgtt ttgtttcatc tggtttgccc atcatgtgga
    34321 acacacgtgt atcttcattg gcataatcaa agtagacata agcttccata cgacgttcat
    34381 gtgtatgaca aggcatcgta ttccaagcgc ttcctggttc taaaatcgta taacccattt
    34441 gtaattgaca gctttcgcat acatttggat ggatgtattg gtagatttta cgttcattca
    34501 gtgtcaggcc ttcaccagtc tccattggtt ttacttggtc gatactgatt tttacatttg
    34561 gatatttatg atgagctgga acagaactaa tatagaattt agctgggttt tcagcatctt
    34621 gagaagaaaa tacaacatgt cgtgtttctt ttccgatata gtatccgtct tgttttttca
    34681 tttcttcttt ttgcccatcg atctcgatga atcctggtcc accgatattg atcacaccta
    34741 gttcacgacg ctctaagaag taatcaacac ctagttcttt gtctaatgtg atttctaatt
    34801 ctttttcagt aggtgtcact ccaccaaaga tcattcgatc gttgtgcgta taagtcaaac
    34861 tgattttccc tggttcaaat accttttcta ctaaaaattc ttttctcaat tgttccgttg
    34921 aataatgacg gatatcttct ggactgtgtg tataacgtgt ttccatttct tgcattttct
    34981 tctactcctt ctatcgaatg atttttccag agccgcatgc taaaaattgt gcgacttctt
    35041 cttgactaaa ttggttgcag tccccatgga tcgtatgctt caaggcagaa gctgccgtag
    35101 caaaatcaat gatttcttgc ggagacatat tggataaaat cccatggagc acgccacctg
    35161 caaaagcatc tccgccacct actcgatcca cgattggggt gattctatgg acttgggatt
    35221 ccacatattc atggttcatc cacaatgtcc ccttcaattc attttcactt gctgaatgaa
    35281 cggtacgctt ggtcgaataa aacacctgaa tattcgggaa cttttcctgc attttttgat
    35341 aataataaat tagaggttgt tcttctgctt ctggcgcttt ctcgatacct aacaaataaa
    35401 gagcatccat ttttccagct gagcagatat ctactaaagg taaaagctga tggattactt
    35461 cgctggcttc ttgctgtgtc cataattttc cacgatagtt gatatcgaag ctgatcaggc
    35521 agccagcttt ttttgcagct tctatcacct gtttcgtcat tttttgccat tttttagaca
    35581 aagctggggt gattcctgaa acatgaaata tatctattcc ttggaataac tcctctagcg
    35641 accattccaa atgatccatt tcagcaaaac ttgatccggc acgatcatag atcactgagg
    35701 cggcgcgttc gccactacca gtttccatat aataagtacc tagtcttgat ccaccagata
    35761 atacctgttt cgtatctact ccataacatt gcaagtgctt tttgactgct tttcccaatg
    35821 cgttatcagg tacttttgta gcaaaaatcg cttcatgtcc gtaattagct aaagaaattg
    35881 ctacgtttgc ttcccctccg ccataatggg cagcgaatcg ttcacttgaa tcgatacggg
    35941 ttccctgctc tgtggacaat cgcaacatga tttctccgag tgtcactact tttcccataa
    36001 gcagctacct cttgatttct ttcatttttg ctgcatactg ttgcgcgact tccgttactt
    36061 tgtcaaaatc tcctgtagct gcgggtgcta aaaggttccc acctactccg actgttacga
    36121 caccagcatc gaaccactct ttcatattgt ccagactcac accgccagtt ggcatgatat
    36181 tgacttgagg catcggcgcc ttgaaggcag aaatgatact tggtccataa gcgcttccag
    36241 gaaatagttt gacgatatct accccgcttt tcagcgctgt tttgatctcg gtaatagtca
    36301 tacatcctgg taagtaagga atttgataca gattgcacat ctctgccgtt tcttgatcaa
    36361 aggaagggct aacaatatac tctgcgccag ctaagatagc caagcgagct gtgacagcgt
    36421 ctaatactgt gcctgcgcca atcactattt ctggttggtc tttgtagaat gacaaaagtt
    36481 ctttgattac ttgatctgct tgagggactg taaaagtcaa ctcaatacca gttagaccac
    36541 ctttgatgat tgcgtggcaa gcattcaaag cttcttcttt gctttttccg cgtaccaccg
    36601 caatcactcc ggcgtttttc aatcgagata aaatatcaat ttttttcatc ttctccacct
    36661 atttcctatt tagaaatttt attttataac ttggaaatct atacccatat tatacggcag
    36721 aaaagaaaaa ttgcaaacgc ttttttcatt atttttgaat tctttgctcg atttcgcttt
    36781 ttgtttgctt gagggcactg attatttgtt cttttgtctc atctgttaaa cgatatttcg
    36841 gcatacttac actaaatgca ccaatgattt gttcgttttt cacgattgag gctcctatac
    36901 aaaaaatatc acgttcgatt tcttcatcgt caaatgcgat gtgggtttct tttaccgttt
    36961 tgatttcctt tttcaaccgt aatgcattgg tgatcgtata ttctgtgtaa gggatcaacg
    37021 gatgcttttc aagataatca gtgtactgct cttcactgaa ttctgctaat acggctttgc
    37081 ccatcgcaga attgtataat ggtcgggtga ttccgatttt tgaagacatg cggatcgttt
    37141 gatgttttgg atctagtttg ttcacgtaca agatctcatt gttgtccaat acccctaagt
    37201 ggatagtctc atcgatcttt tgctgcagct gttctaaaaa cggtaaagtc aactctacca
    37261 aatcgatttg ttcaatgttt ttattcgcgt agcgaataag ctttgcacct aaacgatagt
    37321 ttttctctga atttttattt acgtagccga tcatcaacaa agtatctagt attttcaaga
    37381 ctgtagaaga ggtcattccc acaccctttg cgatcgtctg caaagagaca tcttggtttt
    37441 ccgctaaata gtctagtatt ttagaggctt tgatcaatac agttccataa ggctttttat
    37501 tttccatata atattctcct atttctttat aagaaaaatt atagcggaaa taccatcaat
    37561 ttaaaagaga tatccagata taaactacca tttctcttta caggcaattt aatgttgaag
    37621 atccgcttcg tttttacatc aaactttgat ataaagcaat gatttcttct actgtcgttt
    37681 ctcttggatt tcctggtgtg catacatctc gcaaagcatc ttctgcaatt gctggaatat
    37741 ctgcttcttt tacacctaat tcagaaatgg tttctgggat tccaaccgct ttgctcaacc
    37801 gatcgatctc accacaagcc gcttctctta catcttctaa agacatttca gctgctcctt
    37861 taatccctag aacaagagcg atctcacggt acttttctcc agtatattct ttgttatatt
    37921 tcatgacagt tggcaaaagt gctgcacacg ctacgccatg aggaatgttg taccatgcac
    37981 ttaacgggtg agccatcccg tgtaccagtc ctaaaccgac atttgagaag cccattcccg
    38041 caatatactg tcctaatgcc attttttctc gcccattttg atcgccatct acagaagctt
    38101 ccaaagaatg gccgatgatt tcgattgctt ttaaatgcag catatcagtc atttcccaag
    38161 cgccttttgt gataaaccct tcaatagcat gcgtcatagc atccatacct gttgatgcag
    38221 ccaatttttt cggcattccc atcatcatat cgctgtcgat gaacgcgacg attgggatat
    38281 catgaggatc aacacaaaca aattttcggt gatttacttc atctgtgatg acgtagttga
    38341 tcgttacttc tgctgctgtt ccagaagtcg ttggtatagc taatataggt aaacatggat
    38401 ttttcgtatc agcaacacct tctaaactaa tgacgtctga aaactctgga ttcgtcaaaa
    38461 taatcccaat ggcttttgcc gtatcgattg gcgaacctcc accgattgcg atcaaacagt
    38521 ctgcctctgc ctctttaaca aaagccactc catcttttac attttggatc gttggattag
    38581 gtacgatccc gtcgtataaa gcataagcga ttgctgcttt atctagaagt tctgttactt
    38641 ttgttgctat accgtgctcg atcaatcctt tgtcagtaat gacagctgct ttgccgaatc
    38701 ctcgctgctg aaattcaggg acgatcgact caatcgctcc ttttccaaaa tacgatgttt
    38761 catttaaaat cattcgatta ctcattttct attcctccta atttgtatta tttttcacaa
    38821 taaaaacgaa agaaccccaa gcaaccggtt gcccagttgc ttgttgctac agttcaaaga
    38881 ctggcatcaa ttcccaactg actggagagg catctggatt tgtctccatg atatcttcca
    38941 tgtacttcca ccatttttga ttgatagatg tatttgccat tttttcccat aacttttcat
    39001 cttcgatcac taagtaacca aataacaaac ttgtactcgg atcaagatag atcgaatagt
    39061 cgatgacccc gtgttcacga agtgctgtac gcatttctgg ccaaagttca tcatgtcttt
    39121 ttttgtattc ttcttgtttt tctgggtaaa ctttcatgca aaaagctttt ttcaccatgt
    39181 cagacacctg ctttcgtatg aacttcctcc aaataacctg cttttggagt gacaccaaac
    39241 gcatctgcta aatgccacag gttttcgtcc gtgattgttt gcaagattgg tccttgcgct
    39301 ttgacgtacg tatagacttc tgctgccttt tctgctgtct cgatcaaacc aaagacttca
    39361 tccatgtctt ttcctgaccc gtagattcca tgttgtggcc ataaaaccaa tcgagtttct
    39421 ttcatttttt cagctgttgc ttccccaatc tcatttgttc ccggaaccat ccacggaatg
    39481 atggaaattc cttctggaaa aacaactaag cattccgtac acatttgcca tagcgtacga
    39541 ctaaaggaac gttcgtctaa ttcatgcgta aaactcatcg ctaatagatg actagcatgg
    39601 caatgcataa cgatccgatt atctgggtct acttctaaac gggcgatatg actcatgaaa
    39661 tgacttggca actcgcttgt aggaaggcat tcgttttcca gcccccacaa aagttccagt
    39721 gttttgccat cttcagcaac tcgaacgatt ccaagattct cggcaggttc gctcgctaca
    39781 tttttaaaat atcttcctga accagttaca ataaaatagc atcctgctaa acggctggca
    39841 tcaaaaatca tcgagatgcg gcggatcact ttttcaggat ccaagaacgg tgtgatctct
    39901 tcttctttta gtaaataaga aatattcccg ccatttcttt catcccatcc caagcgataa
    39961 agattcgacg tcgtttcgat catttctttc acataaggcg cttgtaaaat atctattttt
    40021 ttcaatatag ccacactcct attttcttaa ttccaacacg tctttttgat attcctggac
    40081 ttcttccagc caatccaaac caactggaac ttgatttttg taacagaaat aattccatac
    40141 atcggcatac gggaaatctt tcaattcttc cgtataagcc attcttgaag taaagtcaaa
    40201 agccagctcc atttcttcca atcgctcaac tggttccagc atagctttca gcagtgcttt
    40261 ttgtgtattt ctcgtaccga tgacccaagc agcgatacgg ttgatcgttg catcaaagaa
    40321 atccagaccg atatttgttt tttccaacaa ttcgttacgg actaattctt tagcgatttc
    40381 ctgcaattca tcatccatca aaaccacatg gtctgaatcc caacgcactg gacgggaaac
    40441 atgcaacatg attcctttgc tgaataaagc taatgaagat aatttattgg aaatcacttc
    40501 tgttggatga aaatgtccag catctagaca aacaagtttg tcacgagtca acccgtaacc
    40561 catataaaat tcatgggaac cgactgtata agcttcagcg cctaaaccaa atagtttgct
    40621 ttctactgca tcttgtacat accgttcatc taactcttct gtaaaaattt catcgagtga
    40681 ttccattaaa cgttcccgtg gggtaaaacg atcaatcgga ttatctttca tcccatcagg
    40741 tacccaaaag ttattgatac ttgtttgccc agtttctttc ccaaagtact ccgcgatttt
    40801 gcggcttctt ttcccgtgtt cgatccagta ttcacgaact tctttatctg gatgagccaa
    40861 tgtaaagccg tctttcatca ttggatgcga aaagaaagtc ggattgaagt caagaccgat
    40921 ttcttctttt tttgcccact ctacccattt ttcaaaatgt ttcggctcaa tttcatttag
    40981 atcgacagat tcttctgtat ccaaatagat agcatgaagg ttcaatttgt gtttgcccgg
    41041 tatcaacgaa taagccttct ccagatcttg tctaagctct tcaggtgttc gtgcggctcc
    41101 tggaaaattc cctgtcgcca tgattccacc ggtcaattcg ccatccggtg tcaagaaacc
    41161 tttgacatca tctccttgcc agcaatgcat agaaattttg atgtcctgta tcttttctag
    41221 tacagcatct gtatctacac cgatagttgc atatttttct tttgcttgtt cgtaatttct
    41281 ttcaatcgtg ttcatacgat tactcctttg attttattca cgtaccattc catcgtgttt
    41341 tgcttatttt catctgggta aaaagtttca catggaaacg actgtttgat tacatttctt
    41401 gcttgttcta tattcgaaaa ctgacctgta ctgagcattt gcatggcaac attcccgatt
    41461 gctgtggctt cactaggtcc tgcttctaca atgattccgg cataatctgc tgtcaattga
    41521 ttcagtagac gattattcga tccgccccct acgatgtgca gcttttccaa tttcgttcca
    41581 gttaagtgtt ctactttgat cagctcttgt gcgtaacaaa gagcaagatt atcatacaca
    41641 caacgagcga tagctcccga tgagacaggg actggctgct ctgtttcaaa acagtattgc
    41701 tgaatctctt ctatcatatt tgggggattc aaaaatcgag ggtcattgat ttccatcact
    41761 tgctgcagcg gtttttcttt tgcagcaagc tctgccaatt ccgcataact gtactcgtac
    41821 tcttgcattc gggcaacttc ctggatcaac cacatcccca tgatgttttt caaaaaacgc
    41881 accgtgttat gggcacccca ttcattcgta taattttcag aaaaagcatt gcgagaaaca
    41941 ttggcgatct ttgtttcaat ccctaaaagc gaccatgtcc cactgctgat ataaccccaa
    42001 cgttctcctt gacctggcgt tcctaataca gctgacgccg tatcatgact tgctactgtg
    42061 ataaaggtca catctggcaa atcatatttt gaaaattgtt ctttttgcag atctcctagt
    42121 atcgttcccg gctctgtcaa tggtggaaat aattccttcg agacaccgat tttttcaaga
    42181 agttctattt cccattcttt tgtccctgca ttcagcagct gcatagtaga tgcattagtt
    42241 ttctccgtca ccatgttacc agtaaagaca taccctaaat aatcaggtat cagtaacaat
    42301 ttatgtgctt ttgccaaatt ttctttttct tcagcaaaca attggaacaa ggtattgaaa
    42361 ggctggatct gaatccctgt ctgctcatac aacttatcta gagaatagat ttcagaaaac
    42421 tgttctactg ctccattggt acgcttgtcc cgataagcaa tcggatcatt tatccgatcc
    42481 ccttgttcat caatcagaca gtaatcgact gcccatgtat cgatccccgc aaaaaggcga
    42541 tcaatccctg tctgctttaa tttttcaaat ccaatcaaaa tctctttgat aagataatcg
    42601 atcttccagc gatcgtgacc gtctttgttc cgaaagccgt ttttgaaccg gtgtacttct
    42661 tccaagatca aagaaccttt tgcatccctc gtacttttca ttagtcgtcc gcttgatgca
    42721 ccgatatcga tggcaacaaa tgttggattc atttctccac ctcttttact ttattgttga
    42781 ttagcacttt attaataaag cgttatcatt tattgacaat gctatcataa tacagaatct
    42841 tatattttgc ttcacactat tttggtatat aattgcatta tcttgacaag ttgggagtgt
    42901 ttacatggat tactgggaaa aagccaacca ctcatggtcg gaagactcaa aaagagtgat
    42961 cttgacgcct acacaaagaa gcaggaattt atttttttat atccaagaaa tcggttactt
    43021 caaagcacca atcccttact atactgaacg agcacattta ccttcttact tgataaagtt
    43081 tactttgtct ggcaaaggtt cgcttacata taatgataat cagcaacagc taaaagctgg
    43141 agacttattt tttattgact gccaagaata tcagcgttat caaacgatca gcgaagaacc
    43201 atgggaaatg gattggatcc atttgaatgg tcagaacgtc caatcatttt atcaggagtt
    43261 cacaaaagac ggccataaca ccttccattc aacaggagat ccatcagaaa acaagatcca
    43321 ccatttgatc acagaattga tccacatgca acaaggcacg aatgcccgta ccgatttcca
    43381 gtcctctgta gctatccacg agttgctgaa tgaactgatt ttgcaaaagt atcagctaga
    43441 tttcgaagaa gaggatattc ccgcctacgt ttacaaactg aagcaatact tagacgaaca
    43501 tttcaaagaa acgattacac ttgatcaatt agaacaacaa ttcttattga ataaatatca
    43561 gctgagcaaa gatttttcca aatttatcgg ttctccacca atcgattatt tgatcagcaa
    43621 aaaactttct tacgctaaag accttcttcg gtatacagaa tttacggttc gaatgatttc
    43681 cgaagaaata gggattgaga attttgctta ctttagccgc ctgttcaaag caaaaacagg
    43741 actatcgccg agagactttc gtaaattcag ctgattcata taatatgtac gtcatttttt
    43801 caaaatattt tgaaatatat agataaaaat aaaataaccg cagtttctgc ttctatgtaa
    43861 acaacagcag ctactgcggt tattttttag tgatcattct cattgttcat cttcaaaatg
    43921 ttcataatga accaactgtt tctgtgtatc acgtttcaaa aattctttag gcgttatgcc
    43981 gaactctctc ttgaatgctt tcagaaaatg cggataagaa gaaaaaccac ttttactata
    44041 tacagcacta gagctgcggt attctcgcaa caagtgcttg gcatagatca gctttttctt
    44101 cattacatac tgatgaaacg taaatccagt gtacttttta aattctcgtg tgatatggta
    44161 tttgctgaca aaaaactgct tttccatttc ctctaaagaa agcggatgag ataaattttt
    44221 tgagatatat tgtaacattt gttccacacg ctcattagga actacagcat tctgttcttg
    44281 cgcactctct tctttttgta caacttgatt gagaaaaatc aagtattcta ccagagcttg
    44341 ttcatatttt atgtcttcac cgtaaatttt tttatcagga ggatggtcga taaatgataa
    44401 tttttgcttc aatacttcct gttctatttg caatacacga ctcctcttct ctcccattgg
    44461 ttgaaaacaa gccgatagat tagaccatgg cgtggaacga ctttctagaa aagaaggggt
    44521 gacaaagata tagacacgct cgaatacatc tgaagattgg cggacgatac gatgcaaatc
    44581 attggaatgg atgagcagca cacttcctgc tttgatcgta aattgtcgtc cgttcaaata
    44641 aaaagtcgct tctcccttta atgtagcatg gatctcatag aaatcatgat gatgatagat
    44701 cgttttattt tggctgacga catctttgac atgatagatt tcatactgat cagaaaccat
    44761 cgtattgact tgttcaccgc tgattttctt ttggagcatg tgccgcacct ccttttgttc
    44821 acttatgcac aattttatta taccacgttt tttgtaatca aaaagagctg cttttatata
    44881 ctcttataaa aaagaagaga cgtggcatct tctagccccg ccccctccat tctaaatatt
    44941 agttaaccaa cgtcttcttt acattattca agaagacttc ttccataacg aatcccatca
    45001 tcttttgttt cttcaagaac cacatacagg tacggacgat tcttcatgat gatttctttg
    45061 atttttgtgt actcgatcac accgtctccc aaatttaccg gcacgatttt ttcctgttcg
    45121 acacgaaaat ctttcaaatg aaccgcacat atcttttcac caaaacgttc aaaagcttct
    45181 tctaccaatt ggacttgttc ttgataatca ctacttgtga tcaaattggt cggatctaaa
    45241 atgattccta gatagtccga atctactgct tggatcagct gctctaccct tgctaaagaa
    45301 tacaccggat gattcaaacc tggttctatc cccaccatca tttggtattt ttctccagct
    45361 tttaccaagc gacggataac ggacacagct tcagcaaatg cttcatctgt gaaattttct
    45421 tccgtatatt ggatttccgg caaaacacat ccagtttccg aagcaaccat cgacgcacca
    45481 aaggaagaag catagcgaag atactgctcg aactttgtca gcaactcttg tcgtatcgtt
    45541 aaatctggat gaatcatatt gatatagcaa cttaaaattc ctacggtcac ttcttgcttt
    45601 tctaaaactc gtttgacata atttcccatc ccagaattga tctcatttgc gccagaaggt
    45661 atttcaggaa acgaaagacc taaggcgagc tgcagtgtat gaatcccttg ttcttttgtt
    45721 ttttctgcaa gatcttctat cgtttggact tcggaaagat catgtcctct aattgtaatg
    45781 ttcaacataa ctattctcct aactcagcat attgggtagc cacatgacga tttgcgggaa
    45841 gtaagtaatc aaaaacaaga cgactaagat aaccccataa aaaggaagaa gcggtttcag
    45901 cattgcctct gcttttacgc ctcctacact tgcaccgaca tatagtcctg tacctactgg
    45961 cggcgtgatg gagcctacac acagattcat gattgaaaac aacccgtaat gtacaggatc
    46021 aacccctaca cttgtgacga ttggtaaaaa tattggtgta aaaatcaaga tagcaggcgc
    46081 cacatccata aacataccca ccagtaataa cacaagattg actaataaca gaataataaa
    46141 tttattatcc gttacgccca taatcaacga actaattgcc tgaggaatac cggtaaacgc
    46201 catggcgaaa gacatcatgg aagaagtagc caataagaac atgatcgtcc ctgacattgc
    46261 tactgattga aacaacatat taggtatatc ttttagtttt accgttttat agtaaaacat
    46321 agcaatcaac agggagtaaa tgacagctat cgctgaagct tctactgctg taaatatccc
    46381 cgtcaagata ccaccgataa tgatgacaat caatagaagg ctaggaatcg cctcttttat
    46441 tatttttcca gcatgagcca tctgtccttt ttcgacaaca gggtatttat tctttttagc
    46501 aacgatgaat gctactagca tgattcctag cgcccacaaa gcgcctacga gataacctcc
    46561 cataaacaaa gcagagatcg aagcaccacc agcaacaagt gaatacatga taaaagcagt
    46621 acttggagga atgaccatac ctgtcggagc tgacgcgata ttgaccgctg tcgcaaactt
    46681 ccggtcatat ccttcttcca cttgctgagg gatcaacact ccgccaatcg cggtagaagc
    46741 ggcaatggcc gagctagaga tggaaccaaa taaagcattc cctaacacat tcgtatgagc
    46801 caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa tccactagtt tcttagctat
    46861 ccctccatta ttcatgataa tgcccgagag aacgaaaaac ggaatagcga tcagcgaaaa
    46921 actattgata cttcccacca ttttttgagc tgaactaaaa gcagaaacat caaacggtat
    46981 aactaacaat aaagtcatca tcgatgagaa tgcaatactt atagcaatcg gcatccctaa
    47041 tacaagcaac acaacaaata caaaaaataa cactaatccg gcttctattg ccattttatt
    47101 cctccatttt tatatttttt cttgaattgc tggcttgttg cttaatgtat cgattagatt
    47161 gatcaggcta tagataatga tgagtacccc cgatacaggt actgacagat aaacatatcc
    47221 cataggaata cttaaagaag gcgagatctg ccccattgtc aatgtgacag cattccatcc
    47281 gccatacacc atgatcatcg cagcaaatac cagaaataaa ctttgaacaa caacttctaa
    47341 aattttactt tttttgccag ttaatttttc acttaaaacc gttacggcta aatgtgcttt
    47401 ttgtcccaca acatacgcgg aagacaacat ggcaaaccag acaagaccga atcgaacaag
    47461 ctcttccgtg accgtatttg gatttcctaa aaccgttctg gaaaatactt gccataaaac
    47521 aacagcgacc atcccaacaa gcaaaacagc tcccaggatc tctaatacct tgtttaaaat
    47581 cattttcatc acagctacgc tccttgctct tggatctttt gataaaggtc tttgtaggta
    47641 ggatcgtttt tgaatatatc atgaagtggg gcaactgcct ctcgaaacgg tgtattatct
    47701 acatcattga attggactcc atgttcttct tgtgctaaag ctttttcttc ttcaattgct
    47761 tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg ctgcttcttc aatggctttt
    47821 tcctgctcat cagttagctg ttctttgatg gcatcattca tgatgatgat atctggcaca
    47881 cgtgtatgct catcatagga atagtattta gccacaccac catgtccagc tgtatacaag
    47941 acaaattcat tgttttctga tccgttgatc aaattggatt gaagagacgt gtacacttca
    48001 tcacttccca taggtacggg cgaaccgcct aaaagctcta ccattttgat agcggtttcg
    48061 ctttgcatca cacggatctt ctttcccttc aaatcttccg gcgtatggat cggaccgtct
    48121 accatataaa agctgcgctg accagaatca taataggtca atccggtaaa tcctaaattt
    48181 ttggtcgatt ggtagattgg ctccattatt tcttcattat ccatcacctt aaagaaatgt
    48241 tcttcatcat tgaatagata aggaactgaa aaaatcgaat aatctttaga gaaactttcc
    48301 aacgctgagg cacttacttt tgtgaaatct actgctcctg actgtgtcaa ttcgatcagt
    48361 tcacgttctc cacccagttg tccatctggg aaatactgga tctgtacgga accatctgtt
    48421 ttttctttta ctaaacgccc aaattcattc attgcatcaa ttactggctg gctgttgctt
    48481 gcataagcaa agcgcaaggt aactatctca tttacatctg catctgcgac agttgccaca
    48541 ctagaattat ttccgcagcc gcttagacct agcagaccta ggctaagcga acttgctgca
    48601 aacagcagtg atgttttttt catatgggtt tcctttcttt taacaataag agacgagcaa
    48661 ttctgtcaaa caaagcaccg ttaacgactg tccataaggc attgctgtga ttccgatatt
    48721 ccgataaaaa tctaaactgt cccccattcc tgtcccgatt gaaacgtttt gaacttctcc
    48781 tttttcatcg atttgttcaa gcaatccttg gatagcacga taagcgactt ctttatattc
    48841 ttgtggtaaa taacgtttat ggaccgcttt caaaataccg taagcaaatc ctgcagttgc
    48901 agaagattct acgtaagaag aaggatcatc caacaacgta tgccataaac cgctttcaga
    48961 ctgataagtt tttaatgcac gcacttgtgc ttcaagtgtt tcgatcaata atttgcgcaa
    49021 tgcatcttct ttcgataatt ctaagatttc aatgatctct ggaatagcaa tcgtcaacca
    49081 gcagtttcca cgagcccaaa atgcttcggc atagttatga tttccttcaa acgtccagcc
    49141 gtgataccac aatcctgttc gtttatccat caagtatttg atatggatca taaactggta
    49201 acgagcttct tctaaatatt ctgggcgatt caacagtttc ccgatttttg ccaaaggaag
    49261 cactgtcatc atcaatgtat cgtcccacaa ctgattctta ttttccggtc catatgtggc
    49321 atgctgtaac ccgtcttcat ttgtccgagg catgtcattc atgacccatt ctgcccattg
    49381 ttctaaataa ggcaaatagc gtgaatcttt cgtgtcttca tacaagtaag ccatagtcaa
    49441 tagcggtgcc attgtattga cgtttttggg cggagcgcct tcttccatac gatcctcaaa
    49501 ccattcattg accacttgat aagcgcgagg attttttgtc atctggtaat ttttataaat
    49561 accatataga ccgacacctt gtggccaatt ccacactgtc cagcttttgt catctacttt
    49621 cagtccatca aagtccaaca aaaattcacc attatcgtct ttgatcgttg tcaggttttc
    49681 aacgagcagg tcaatttttt ctttcactaa ttctttaggt acaaaaatct cttctttaat
    49741 aatcatcttt acgccacctt tttaaacgct ttcattttat atccttaata tagcagtaat
    49801 accgtccact ataaatatcg ctttttgaac aaaaactgtc taaaattgac ctaaatgtta
    49861 aataacaaaa gtaactttca gttacttgga aattaaaaaa agaacttgga gaaacaacgt
    49921 tttccaagtt ctttttcatc tattttctta tcttttttct acgctttctt ttgcttatcc
    49981 gttgtaagca tcaatgatga tctgtttcaa ttcagagatc aatggttctt tcggattagc
    50041 tgtcgtacat tggtcttcgt aagctaattc agccatacgg tctacggttg tatctagtgt
    50101 ttcttgtgtg actccttgag cttttagact catgttgatg ccgacagatt ttcctaattc
    50161 ataaacagcc gttgctaaag cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa
    50221 gaattttgcg atatctgcgt aatctgtatc tgcacggaaa tagtcatatt tagggaacat
    50281 cgcatgtttt tgtggatctt ttgcattata gcggataata tgcggtaaaa gaatcgcatt
    50341 tgtccgtcca tgagggatcc cgtattcccc gccgatttta tgtgcgactg agtgacaaat
    50401 acctaggaat gcgttagcaa acgccatacc agccattgta gaagcgttgt gcattttttc
    50461 acgtgattcc atatcaggtg tttttactga tttttctaaa tagtcaaaga ctaacttgat
    50521 tgcttgtagg ctcaatccac gtgtataatc tgaagccatg actgaaacat aggattcgat
    50581 tgcatgtgtt aacacatcca tcccggtatc tgctgtgaca gatgctggaa ctgacatgac
    50641 gaattgagga tcgacgattg cgacatctgg cgtcaatgca taatctgcta acggatattt
    50701 cacatgtgtt tcactgtcag tgatgaccgc aaatggcgtt acttcagaac cagtccctga
    50761 tgttgtcggg atacatacga attgtgtttt ttctggtttg tcgatcttat atgtccgttt
    50821 acggatatcc aagaattttt gttttgcacc aaagaaagat gtgtctgggt gttcatagaa
    50881 catccacatt cccttagctg cgtccattgc agaaccgcct ccaagagcga ttactgtatc
    50941 tggcttgaag tctgccatca gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg
    51001 ttcaacatct gagaagactt cgatcttcac atcattttta cgtcgtgcca atacttcacg
    51061 aactgtatcg cagtaaccga attggaccat accagggtca caaacgatca tgacacgttc
    51121 aacgttttcc attttctcta agtacaatag agagttcttt tcaaagaaga tttttggcgg
    51181 taatttgaac cattgcatgt tatttctccg tttcgctaca gttttgacat tgattaagtt
    51241 tacagcagag acgttacgag aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa
    51301 tgacggaatc atttcgttat agatatcacc gatgccgcct tctgcagatg gtgtattcac
    51361 taaaatacga caagctttca ttcgaagacc gaatttgaca tgcaactctt cgttttctga
    51421 atggataact gctgtatgtc ctaatcctcc aagattcaac ataccttcac acaaatcaaa
    51481 cgcatggtcc gtgttgttag ctttgatcat ggcaagaact ggagaaagtt tttctcttga
    51541 tagcggataa tctgggccta cgccttccaa ttcagcaatc agcattttcg ttccttttgg
    51601 tacactgatt cctgccaatt ttgcgatttc catcgcagaa tgtccaacga ttgatggatt
    51661 gaccgcatat tttccttcgt tcatcactgc atcttctaat ttttgcaatt catctggttt
    51721 tacgatataa acttggtgtg cttggaattc tgcttttact gctgcataga tttctttatc
    51781 cacgatcaca gcttgttcag aagcacaaat cattccatta tcaaatgttt tcgaaacgat
    51841 caaatcgttc actgcacgtt taatttttgc tgttttttca atataagctg gtacgttacc
    51901 tggacctaca cctaaagctg gttttcctgt agagtaagca gacttgacca ttccagctcc
    51961 acctgtcgct aagacgatcg cgatacctgg atggttcatt agcatagaag tcgcttcgat
    52021 cgaaggatgt tcgatccatt ggatacaatt ttcaggagct ccggcagcga tagcagcatc
    52081 acgtaccact cgtgctgctt ctgcagaaga tttttgggca cttggatgga aagcaaaaac
    52141 gattggattt cttgttttca acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt
    52201 cgttgttgga gttactccgc aaaccacacc gactggttca gcaatttcaa tcaacccttt
    52261 ttggacatct tcattgatca caccaactgt tttatcatgt ttgatattat gccaaatata
    52321 ttcggacgca tacatatttt tgattgcttt atcttcgtaa acaccgcggc ctgtttcctc
    52381 tacagccatc ttcgctaatg gcatatgttg atccaaagct gccattgcca tttcatgcac
    52441 gatatggtct actttttctt gatcaaagct ttccatttct ttcaatgctg catttgcttt
    52501 ttctgccaaa tcatcaatca ttgcttgtac gtcaattgtc ttcgtttttt ctttttctat
    52561 tactttagcc atcattatcc tcctaatggg tcatggttat ttgttaaata attcacatgt
    52621 atatcataca atatttattt ttctatgtaa acactttttt gtgatttttt tcacagttat
    52681 ttttaaatat aaaattgctt tatttacagg tgtttgtata gaaaaatata taaagttatt
    52741 tattattatt tatatgacac acccgattat tcaatattga gcaatatatc ccatgctctt
    52801 atctctttct tttttgaata aaaaatgagg taggacatct ttctgcccta cctcgtcttt
    52861 aaataagctt tgatacttat ttttcttcct tctcttctgt ctcttccagc gtttctgttg
    52921 tttcagtttt tacttcaact gtctcagctg gttcttctgt tgtttcaaca gtagtcgttg
    52981 catcatttgt tacgacagtc cctggtttga ccgttttgat tgctgcacga tcgtactcaa
    53041 ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt tctgaaacaa
    53101 ctccatgtaa tccgccgatc gttacgactg aatcacctgg tttcatgcta tctaacaagt
    53161 tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac attgctaata
    53221 gcgcgataaa catcacaagc attggtaatg ttcccatttt tttcacctca ctaattctgt
    53281 atctacaaaa aacactttag cagaaaaatg ctgataacac tagtttttta cttaattttt
    53341 aaaagatttt cctttagaag ctttttgcgt tttctttatt gaacccgtat tcttcaaaaa
    53401 aggcttctct aaattcaagc aaattatcat ccatgattgc ttgacgtact tgtttcatta
    53461 gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat gtttcatcac
    53521 atttgatcaa atggcggata taggcacgtg tatagttttt acatgtataa caatcacatt
    53581 tctcatcaat tggacggaaa tcatgggcat atttcgcatt tttcactact agacggcctt
    53641 gagaagtcat acatgtgcca tttcgagcga ttcgagtagg caagacacag tcaaacatgt
    53701 ctactccacg gataacacca tcgatcaatg aatcagcagc accgacaccc atcaagtaac
    53761 gtggtttgtt ttcggggatc aacggtgtag tgaattctaa tacacgattc atttctgatt
    53821 taggttcccc gacagataac ccgccgattg agtaaccagg gaaatccatc ccgactagat
    53881 ctttcgcgct ttgacgacga agatcttcaa accctgctcc ttggatgatt ccaaataatc
    53941 cttgacggtc tggattagca tgagctttca atcctcgttc tgcccatcga gacgtacgtt
    54001 ctactgattt tttcacgtag tcgtaacttt catcgaatgg tggacattcg tcaaaactca
    54061 tcatgatatc agagcccaat ttattttgga tgttgattgc tttttctggt gataaaaaca
    54121 tacgcgaacc gttcaagtga tttttgaaat gcacgccttc ttcttcgata ttccgcatat
    54181 cacttaatga aaaaacttga aagccgcctg aatctgttaa aatcggttga tcccagttca
    54241 taaatttatg cagtccgcca gcttcttcta ctaagtcttc accagggcgt agccataaat
    54301 gatatgtatt acttaaaata acgccagcac ccatttcttt caactcttct ggggacattg
    54361 tttttactgt agctaatgtt cctactggca tgaacattgg tgtggggaaa gttccgtgtg
    54421 gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt aaacgatagc
    54481 gaatggcagg ttgcgtcatt catttcccta ctttctatat aaaatatctt gacaactccc
    54541 accatcataa tcgtttcaag tgctttttgc aagttccctc ggcttccttc tactttattt
    54601 aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac attatgacag
    54661 aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga aaaatcatgc
    54721 aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt tggggacaag
    54781 ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt cttattatga
    54841 ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc tcagaagcga
    54901 caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc atcattagcg
    54961 ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga caaaaagatc
    55021 agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc ggcggagtga
    55081 tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta ttagttattc
    55141 ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac gatatcgtcc
    55201 aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga aaattgatgg
    55261 atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg catatcttag
    55321 cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct gcaacaaaag
    55381 cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt gtgaccgagg
    55441 gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag cccattagca
    55501 aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc agtaaaaaga
    55561 aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat cgatggaact
    55621 tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag aaccagtgat
    55681 tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg
    55741 ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc gctaaattga
    55801 ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat gaataagcta
    55861 agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat gggctgatag
    55921 tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag acccctgcat
    55981 ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc ggaatgggtg
    56041 gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct ggaagtccat
    56101 cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc catcctaaca
    56161 gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa aagttgatag
    56221 ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg atcgtcgcaa
    56281 agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt cctgtgatcc
    56341 ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca ccatccccag
    56401 tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc ttgtcttctg
    56461 gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct tcgtctgata
    56521 attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata cctaattctt
    56581 tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt ttgatgccag
    56641 cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg atcattccga
    56701 tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct tgcggttctt
    56761 cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca aagcgatcat
    56821 taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga acatcgaatg
    56881 ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc atcatttttc
    56941 gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat tctgcctttg
    57001 tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta cctttgataa
    57061 caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc attttcaata
    57121 tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct tctttgctcc
    57181 atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct gagcagataa
    57241 cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg
    57301 ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag gtttccggta
    57361 cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt tctccttgca
    57421 gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg attcgtttgc
    57481 ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat
    57541 ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct ttcccacgac
    57601 ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat gactcatctt
    57661 cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc aacgccgatt
    57721 cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata gcatcaccgg
    57781 actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg gtcactttgc
    57841 cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc agtgcttttt
    57901 ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata gcgatgatca
    57961 gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg
    58021 ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat tttttgatca
    58081 tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc cgttcatcca
    58141 cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt acttcttcta
    58201 ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta
    58261 cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta tcgattattt
    58321 gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt
    58381 atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc gatagaacag
    58441 tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg tagagtacaa
    58501 acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa acaagaagta
    58561 atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg
    58621 tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag aaattcaaca
    58681 ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga atccttccga
    58741 ggtacaagaa agccgagctt atcgagagct cggctttttc tttgtcataa aatctttgta
    58801 aagctttttt gttattcaaa actagcggct tttcataacc ttatttcacg aacatcgcat
    58861 ctccaaaact aaagaaacga tagcgctctt caatcgcatg ctggtaagca gataaagtca
    58921 attctcttcc tgcaaaggca ctaactaaca tgactaaagt cgattttggc aaatggaaat
    58981 tagttgagaa agcttctacc actttgaatt catagccagg cgtaatgaaa atatcagtcc
    59041 atccgctgtc tgctttgatt tctccattaa atttcgttcc aatcgtttct aatgttcgaa
    59101 ttgaagttgt tccgacagcc acgattttcc cgccattttt acgtacttgg ttcaatcgct
    59161 cagccgcttc ttccgtcaaa cggtagaatt cactatgcat atggtgttct tcaattttat
    59221 ctacgctgac aggtcggaat gtacctaacc ctacatgtag tgtcagatag actaattcaa
    59281 caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg
    59341 ctgcagcaga accattttct ttcgcataga cagtttgata gcgatctggg tcttccagac
    59401 gttctttgat atatggaggc aatggcattt ctcccaatga ttcaagaatc tctagaaaaa
    59461 ttccttcata ctcaaagtca atgatccgtc cgccgtgttc caattcttct ttcactactg
    59521 cttgcaatct gccatcacca aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt
    59581 tgatcaatgt ctcccacgta tctccttgtg tattcgttag aagaaggact tctaaatgcg
    59641 caccagtatc tggtttttct ccatataaac gagcaggcag aactcgtgta ttattcataa
    59701 ctagcgcatc accaggctgt aactcttcaa tgatttcatg gaaatgttta tcttccatct
    59761 ctccagtttt ccgatccaaa acaagtaaac gagaatgatc gcggtctttt aatggtgttt
    59821 gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc tgttgttaac atatcgtcac
    59881 tcactttcta tcatgtgctt catcattcta tcatgttttt cgttctgttg gcaaaatcag
    59941 cactttttca cttctatttt ttgttttcct aaataaaaaa actaccctat ttgtattttt
    60001 tcattgtttt aggcatacta gtaatagata gttcttcatt gaaaatagaa aaagaggtgg
    60061 caccatgatt gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga
    60121 ggacgtcaat ctttcatttg aaaagggtga gttcatttgt tttatcggta cgagcggaag
    60181 cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact gatccaacac aagggaaaat
    60241 tttgattgat ggaaaatcca tccaagaaat tgatccagtg gaattgcgac gtcagatcgg
    60301 ctatgttatc caaagtattg gattgatgcc tcacatgacg atccgtgaaa atatcactct
    60361 tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat aaaacagctg aaaaaatgat
    60421 tgatcttgtg gaattgcccc gtgagatgtt agatcgctac ccacatgaat tatctggtgg
    60481 tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat
    60541 ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag accttgtcaa
    60601 agatttacaa gagcgtttgg gaaaaacgat cgtatttgta acccatgaca tggatgaagc
    60661 gattaaacta gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac
    60721 accagaaaat attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga
    60781 tcgcctacta caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc
    60841 aatcacgatt acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg
    60901 tgtagatacg ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga
    60961 aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt
    61021 tttttacgta aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg
    61081 gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc
    61141 atcattagtg gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt
    61201 tgcagagtca gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca
    61261 tcagcacggt tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc
    61321 tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa
    61381 agctgccacg attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt
    61441 attggcattg atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt
    61501 tatttattca ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg
    61561 gaattatcgg gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt
    61621 tgaattgccc attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt
    61681 gattgcatgg gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt
    61741 tagtggactg aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat
    61801 tttagcacta ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc
    61861 acttagagag gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct
    61921 cttcttatac ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc
    61981 atttcgatca caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga
    62041 atggttgaac attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact
    62101 atcaaccatc aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga
    62161 acagatttaa cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct
    62221 accgtgaaag atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatagtttt
    62281 gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa
    62341 gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc
    62401 aatcgtaaag gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg
    62461 atattaccta tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt
    62521 gttcttggct actctacgga tggacggatc gccagttatg atctagtcat gttggaagat
    62581 gatcgtcaat tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct
    62641 gatacgcctc atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa
    62701 atgcaggaat tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa
    62761 cgtttcttac aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg
    62821 tctttcaaca atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta
    62881 tccgtcactt cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg
    62941 ttgggatcat gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata
    63001 tcatccagac gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg
    63061 gagtcaatgt cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata
    63121 cgtataccgg gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga
    63181 tgactgctag acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg
    63241 caggcataag aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg
    63301 gtgctggtgg tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga
    63361 ttattttagc aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag
    63421 gtattttgga aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa
    63481 aacaaaagct tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta
    63541 aggcaataat ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta
    63601 tttatccatt ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg
    63661 actaagtttt tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca
    63721 tggaattcat accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca
    63781 attcttacat cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc
    63841 gatgccttca gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt
    63901 ttatacaatc gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag
    63961 cttctcgcca tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat
    64021 ccgtcgcaac tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc
    64081 aattgatcga tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt
    64141 gatgcattgc cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc
    64201 gtttgagtga cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg
    64261 acactggtat tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct
    64321 gggaaccaat aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct
    64381 gtttctcgat aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc
    64441 gttgccactg ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt
    64501 cttcctcctt gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc
    64561 ttcaaaggtg gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg
    64621 gttttagaac aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag
    64681 gttggctctt ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat
    64741 tttcctcatc aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc
    64801 cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca
    64861 atgtctgaat cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat
    64921 ccactttcgc agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc
    64981 aatatcatcg atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa
    65041 agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat
    65101 caatacagca ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt
    65161 tacctctttc aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt
    65221 agccattgtc tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa
    65281 aaaaatacaa taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa
    65341 gacccctttt tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg
    65401 tagccaaacg taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag
    65461 cctgtgagtc tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat
    65521 tgatatcttg aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga
    65581 ttttggccgt caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt
    65641 catgttcact atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa
    65701 aatggcactg agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta
    65761 tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg
    65821 tacattattt ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta
    65881 cagctaaata ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag
    65941 agggcacctt tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga
    66001 gggtctgttt ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga
    66061 caacgatatt ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga
    66121 gaatattctg ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt
    66181 tttgtttcgc tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg
    66241 agcgataaga agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg
    66301 cttcgatttc tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat
    66361 aatcaccgat cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga
    66421 ctaatacaag aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa
    66481 gctgttctgc tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg
    66541 gcataatgat gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga
    66601 ttgcatatga gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc
    66661 cttctttttt ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc
    66721 cgatagagta taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga
    66781 tcgaacctag cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt
    66841 gatacatgtg aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag
    66901 aaaaaccggt gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat
    66961 acaaaatacc atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt
    67021 caggaacgat cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga
    67081 tcaatgccat catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg
    67141 gtacagcatt actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc
    67201 gtaaaatcaa agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca
    67261 atggcacagc acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa
    67321 ggataaatgt gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat
    67381 tcagcatcat cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg
    67441 ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca
    67501 tgtagtcgat caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac
    67561 tgctgacgac atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata
    67621 gcgttaatga aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg
    67681 ctgcagcaga caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg
    67741 ataacgcatc agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat
    67801 tttcagcaga ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa
    67861 ctcctttaca cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg
    67921 ttatagtacg ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa
    67981 cagaaaaata tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt
    68041 atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt
    68101 ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca
    68161 ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag
    68221 cttgcatctg cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg
    68281 tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa
    68341 tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat
    68401 ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa
    68461 tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga
    68521 ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg
    68581 tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa
    68641 atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc
    68701 cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat
    68761 cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa
    68821 atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg
    68881 taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt
    68941 gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa
    69001 cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag
    69061 ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa
    69121 agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga
    69181 cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg
    69241 gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt
    69301 tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt
    69361 ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata
    69421 ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat
    69481 tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg
    69541 ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt
    69601 ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag
    69661 aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat
    69721 taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa
    69781 cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat
    69841 ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca
    69901 ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt
    69961 tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg
    70021 ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg
    70081 taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt
    70141 ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat
    70201 cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg
    70261 tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga
    70321 tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca
    70381 ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca
    70441 cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga
    70501 ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat
    70561 ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata
    70621 ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg
    70681 aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat
    70741 attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat
    70801 cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt
    70861 cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt
    70921 gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag
    70981 caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg
    71041 catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga
    71101 aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc
    71161 tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc
    71221 ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct
    71281 cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga
    71341 aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat
    71401 tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc
    71461 ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg
    71521 tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc
    71581 tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct
    71641 tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc
    71701 atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat
    71761 acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt
    71821 tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt
    71881 actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga
    71941 cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa
    72001 atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca
    72061 actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta
    72121 agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga
    72181 accaatctct gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt
    72241 tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc
    72301 ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc
    72361 aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc
    72421 cctttttctt taagtttacg tttatgcctt tattttgaca aaaaaaatgt cttttcgttt
    72481 tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat
    72541 ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg
    72601 gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct
    72661 gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta
    72721 gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac
    72781 atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt
    72841 ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc
    72901 gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct
    72961 ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg
    73021 ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac
    73081 taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt
    73141 ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt
    73201 ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt
    73261 ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa
    73321 taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa
    73381 aataaatatc tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc
    73441 cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc
    73501 tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga
    73561 aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag
    73621 tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa
    73681 ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg
    73741 attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct
    73801 ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct
    73861 actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg
    73921 ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct
    73981 ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa
    74041 ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca
    74101 ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc
    74161 gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct
    74221 gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc
    74281 aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt
    74341 gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca
    74401 tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca
    74461 acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg
    74521 tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa
    74581 gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc
    74641 acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg
    74701 atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat
    74761 tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt
    74821 cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca
    74881 tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag aacactctca
    74941 ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt
    75001 tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat
    75061 ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta
    75121 taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt
    75181 ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct
    75241 ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt
    75301 gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta
    75361 tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct
    75421 ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt
    75481 tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct
    75541 tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata
    75601 tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc
    75661 gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt
    75721 tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc
    75781 aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca
    75841 tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga
    75901 tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg
    75961 aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt
    76021 ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac
    76081 atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg
    76141 ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag
    76201 ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg
    76261 tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg
    76321 ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca
    76381 aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat
    76441 gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact
    76501 gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat
    76561 ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta
    76621 ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca
    76681 ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt
    76741 tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt
    76801 ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca
    76861 gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg
    76921 ccttttagca taatactatc tgttgcttca agattttgtt tgatagcatc aatcaattta
    76981 ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct
    77041 tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata
    77101 tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct
    77161 aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc
    77221 atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt
    77281 agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt
    77341 atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa
    77401 cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt
    77461 ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt
    77521 tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat
    77581 ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac
    77641 ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc
    77701 ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt
    77761 tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt
    77821 ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg
    77881 gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa
    77941 taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt
    78001 tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct
    78061 tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg
    78121 gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg
    78181 cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt
    78241 tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt
    78301 tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct
    78361 ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc
    78421 ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga
    78481 gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt
    78541 catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt
    78601 aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa
    78661 tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt
    78721 ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga
    78781 tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg
    78841 ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca
    78901 tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt
    78961 ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac
    79021 cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata
    79081 cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt
    79141 ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg
    79201 gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat
    79261 gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt
    79321 cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg
    79381 aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa
    79441 tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag
    79501 cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa
    79561 cgaacgtcat ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg
    79621 ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat
    79681 cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga
    79741 acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct
    79801 ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga
    79861 tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct
    79921 ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg
    79981 gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc
    80041 taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat
    80101 caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg
    80161 ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca
    80221 aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa
    80281 aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga
    80341 aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc
    80401 aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga
    80461 tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag
    80521 ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac
    80581 ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg
    80641 gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa
    80701 agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga
    80761 tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat
    80821 tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa
    80881 tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt
    80941 gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca
    81001 ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt
    81061 ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg
    81121 gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag
    81181 gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg
    81241 gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg
    81301 gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat
    81361 ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa
    81421 gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt
    81481 tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac
    81541 ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc
    81601 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    81661 gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg
    81721 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    81781 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    81841 ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    81901 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    81961 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    82021 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    82081 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    82141 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    82201 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    82261 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    82321 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt
    82381 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    82441 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    82501 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    82561 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    82621 tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    82681 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    82741 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    82801 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    82861 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    82921 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    82981 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    83041 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    83101 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    83161 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    83221 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    83281 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    83341 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    83401 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    83461 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    83521 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata
    83581 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    83641 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    83701 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    83761 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    83821 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    83881 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    83941 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    84001 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    84061 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    84121 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    84181 ccaatgatcg gcacaaaaac ttccggccat acttcttgta tgactgccat cagctcatcc
    84241 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    84301 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccac
    84361 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    84421 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    84481 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    84541 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    84601 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    84661 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    84721 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    84781 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    84841 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    84901 attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DAJSNV010000002        68378 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSNV010000002 DAJSNV010000000
VERSION     DAJSNV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.002"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            41..922
                     /locus_tag="ON516_000082"
     CDS             41..922
                     /locus_tag="ON516_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343601.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="ON516_000083"
     CDS             935..2545
                     /locus_tag="ON516_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3343602.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="ON516_000084"
     CDS             complement(2622..3281)
                     /locus_tag="ON516_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3343603.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="ON516_000085"
     CDS             complement(3564..4229)
                     /locus_tag="ON516_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3343604.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="ON516_000086"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="ON516_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3343605.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="ON516_000087"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="ON516_000087"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3343606.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="ON516_000088"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="ON516_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3343607.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="ON516_000089"
     CDS             6543..6863
                     /locus_tag="ON516_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3343608.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="ON516_000090"
     CDS             complement(6975..7844)
                     /locus_tag="ON516_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3343609.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..9244)
                     /locus_tag="ON516_000091"
     CDS             complement(7940..9244)
                     /locus_tag="ON516_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3343610.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9241..9750)
                     /locus_tag="ON516_000092"
     CDS             complement(9241..9750)
                     /locus_tag="ON516_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343611.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9925..12060)
                     /locus_tag="ON516_000093"
     CDS             complement(9925..12060)
                     /locus_tag="ON516_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3343612.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12143..13798)
                     /locus_tag="ON516_000094"
     CDS             complement(12143..13798)
                     /locus_tag="ON516_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3343613.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13800..14114)
                     /locus_tag="ON516_000095"
     CDS             complement(13800..14114)
                     /locus_tag="ON516_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3343614.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14284..14544
                     /locus_tag="ON516_000096"
     CDS             14284..14544
                     /locus_tag="ON516_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3343615.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14558..14872
                     /locus_tag="ON516_000097"
     CDS             14558..14872
                     /locus_tag="ON516_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3343616.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15602..16156)
                     /locus_tag="ON516_000098"
     CDS             complement(15602..16156)
                     /locus_tag="ON516_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3343617.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16282..18210)
                     /locus_tag="ON516_000099"
     CDS             complement(16282..18210)
                     /locus_tag="ON516_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3343618.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18516..19232)
                     /locus_tag="ON516_000100"
     CDS             complement(18516..19232)
                     /locus_tag="ON516_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3343619.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19233..21056)
                     /gene="glpO"
                     /locus_tag="ON516_000101"
     CDS             complement(19233..21056)
                     /gene="glpO"
                     /locus_tag="ON516_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3343620.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21070..22566)
                     /gene="glpK"
                     /locus_tag="ON516_000102"
     CDS             complement(21070..22566)
                     /gene="glpK"
                     /locus_tag="ON516_000102"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3343621.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22702..24162)
                     /locus_tag="ON516_000103"
     CDS             complement(22702..24162)
                     /locus_tag="ON516_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3343622.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24152..25447)
                     /locus_tag="ON516_000104"
     CDS             complement(24152..25447)
                     /locus_tag="ON516_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3343623.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25779..26456)
                     /locus_tag="ON516_000105"
     CDS             complement(25779..26456)
                     /locus_tag="ON516_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3343624.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26486..27577)
                     /locus_tag="ON516_000106"
     CDS             complement(26486..27577)
                     /locus_tag="ON516_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3343625.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27678..28112)
                     /locus_tag="ON516_000107"
     CDS             complement(27678..28112)
                     /locus_tag="ON516_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3343626.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28696..30153)
                     /gene="ascB"
                     /locus_tag="ON516_000108"
     CDS             complement(28696..30153)
                     /gene="ascB"
                     /locus_tag="ON516_000108"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3343627.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30150..32045)
                     /locus_tag="ON516_000109"
     CDS             complement(30150..32045)
                     /locus_tag="ON516_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3343628.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32103..33878)
                     /locus_tag="ON516_000110"
     CDS             complement(32103..33878)
                     /locus_tag="ON516_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3343629.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33928..35424)
                     /locus_tag="ON516_000111"
     CDS             complement(33928..35424)
                     /locus_tag="ON516_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3343630.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35618..37240)
                     /locus_tag="ON516_000112"
     CDS             complement(35618..37240)
                     /locus_tag="ON516_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3343631.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37689..39002)
                     /locus_tag="ON516_000113"
     CDS             complement(37689..39002)
                     /locus_tag="ON516_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3343632.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39247..39471)
                     /locus_tag="ON516_000114"
     CDS             complement(39247..39471)
                     /locus_tag="ON516_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343633.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39491..39913)
                     /locus_tag="ON516_000115"
     CDS             complement(39491..39913)
                     /locus_tag="ON516_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3343634.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40056..40766)
                     /locus_tag="ON516_000116"
     CDS             complement(40056..40766)
                     /locus_tag="ON516_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343635.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40784..41281)
                     /locus_tag="ON516_000117"
     CDS             complement(40784..41281)
                     /locus_tag="ON516_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3343636.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41318..41770)
                     /locus_tag="ON516_000118"
     CDS             complement(41318..41770)
                     /locus_tag="ON516_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3343637.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41905..42510)
                     /locus_tag="ON516_000119"
     CDS             complement(41905..42510)
                     /locus_tag="ON516_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3343638.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42960..43580)
                     /locus_tag="ON516_000120"
     CDS             complement(42960..43580)
                     /locus_tag="ON516_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3343639.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43582..44451)
                     /locus_tag="ON516_000121"
     CDS             complement(43582..44451)
                     /locus_tag="ON516_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3343640.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44474..44881)
                     /locus_tag="ON516_000122"
     CDS             complement(44474..44881)
                     /locus_tag="ON516_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3343641.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44900..46189)
                     /locus_tag="ON516_000123"
     CDS             complement(44900..46189)
                     /locus_tag="ON516_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3343642.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46216..46518)
                     /locus_tag="ON516_000124"
     CDS             complement(46216..46518)
                     /locus_tag="ON516_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3343643.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46543..48636)
                     /locus_tag="ON516_000125"
     CDS             complement(46543..48636)
                     /locus_tag="ON516_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3343644.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49125..49889)
                     /locus_tag="ON516_000126"
     CDS             complement(49125..49889)
                     /locus_tag="ON516_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3343645.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="ON516_000127"
     CDS             complement(50000..50869)
                     /gene="mdcB"
                     /locus_tag="ON516_000127"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3343646.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50894..51970)
                     /locus_tag="ON516_000128"
     CDS             complement(50894..51970)
                     /locus_tag="ON516_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3343647.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51994..52587)
                     /locus_tag="ON516_000129"
     CDS             complement(51994..52587)
                     /locus_tag="ON516_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3343648.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="ON516_000130"
     CDS             complement(52591..54267)
                     /gene="mdcD"
                     /locus_tag="ON516_000130"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3343649.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54257..54556)
                     /locus_tag="ON516_000131"
     CDS             complement(54257..54556)
                     /locus_tag="ON516_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3343650.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="ON516_000132"
     CDS             complement(54658..56304)
                     /gene="mdcA"
                     /locus_tag="ON516_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR3343651.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56311..57222)
                     /locus_tag="ON516_000133"
     CDS             complement(56311..57222)
                     /locus_tag="ON516_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3343652.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57342..58199
                     /locus_tag="ON516_000134"
     CDS             57342..58199
                     /locus_tag="ON516_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3343653.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58386..58748)
                     /locus_tag="ON516_000135"
     CDS             complement(58386..58748)
                     /locus_tag="ON516_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3343654.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59094..60533
                     /locus_tag="ON516_000136"
     CDS             59094..60533
                     /locus_tag="ON516_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3343655.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60540..63092)
                     /locus_tag="ON516_000137"
     CDS             complement(60540..63092)
                     /locus_tag="ON516_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR3343656.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63206..64537)
                     /locus_tag="ON516_000138"
     CDS             complement(63206..64537)
                     /locus_tag="ON516_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3343657.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64503..65981)
                     /locus_tag="ON516_000139"
     CDS             complement(64503..65981)
                     /locus_tag="ON516_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3343658.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66286..68121)
                     /gene="tdc"
                     /locus_tag="ON516_000140"
     CDS             complement(66286..68121)
                     /gene="tdc"
                     /locus_tag="ON516_000140"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3343659.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19450 a        14583 c        11702 g        22643 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9241 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9301 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9361 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9421 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9481 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9541 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9601 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9661 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9721 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9781 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9841 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     9901 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     9961 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10021 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10081 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10141 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10201 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10261 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10321 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10381 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10441 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10501 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10561 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10621 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10681 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10741 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10801 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10861 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    10921 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    10981 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11041 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11101 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11161 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11221 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11281 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11341 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11401 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11461 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11521 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11581 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11641 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11701 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11761 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11821 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    11881 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    11941 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12001 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12061 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12121 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12181 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12241 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12301 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12361 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12421 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12481 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12541 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12601 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12661 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12721 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12781 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12841 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    12901 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    12961 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13021 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13081 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13141 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13201 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13261 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13321 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13381 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13441 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13501 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13561 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13621 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13681 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13741 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13801 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13861 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    13921 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    13981 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14041 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14101 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14161 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14221 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14281 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14341 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14401 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14461 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14521 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14581 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14641 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14701 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14761 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14821 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    14881 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    14941 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15001 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15061 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15121 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15181 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15241 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15301 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15361 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15421 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15481 acaagaaata atttcaaaaa caacttctcc tgtttttggt ttggttggct tattatcgag
    15541 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15601 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15661 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15721 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15781 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15841 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    15901 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    15961 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16021 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16081 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16141 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16201 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gccactgata
    16261 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16321 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16381 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16441 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16501 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16561 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctggcgtt cttttttatt
    16621 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16681 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16741 agcaatccac aaagcttggc tttctcctgt ttctccaacc cgcaactgat atttaggacg
    16801 aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa
    16861 tttcttgatt tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga
    16921 ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc
    16981 atctaaaagt accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat
    17041 atgaccagaa aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa
    17101 aatctcatca aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat
    17161 cgccatcaaa gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc
    17221 tccggtgata atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg
    17281 ctcaggtaaa aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct
    17341 aggtgtgctt ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac
    17401 cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa
    17461 tgccgccagg acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc
    17521 attcaattta gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc
    17581 gataatcgtt ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc
    17641 gcctttttga acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa
    17701 tttcagtaat tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg
    17761 caaattacgt gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc
    17821 ttctacattt atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta
    17881 ttgattctta tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc
    17941 gatcaaatcg gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat
    18001 cctcggcaac cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct
    18061 tgtttcttct tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat
    18121 tcgttttttg ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa
    18181 ttgtaattga ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt
    18241 cccaaaataa ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca
    18301 tgtcctatcc ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc
    18361 acaccaaata aactttgttt attggctatt cgacttactg attagatacc agctacactc
    18421 acaaaacata ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac
    18481 attttggttg tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct
    18541 accgcgataa ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct
    18601 gaagttcctt tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga
    18661 gcagggttga ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat
    18721 ccacctacag cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac
    18781 ccaagaacta atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt
    18841 atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa
    18901 tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca
    18961 atgatgaaag gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag
    19021 actgcaggat tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca
    19081 gcgagtcccc aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt
    19141 aaattcacag ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac
    19201 tctcccatga tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga
    19261 tctgattctg caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc
    19321 cattcaaaat atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct
    19381 cgttcaaaca acagatgatt tgtacgacga atgagataat cgcctggagc caaaaccatt
    19441 tcttcttcta aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt
    19501 gccatttctt ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata
    19561 taagtagcat cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact
    19621 gtttcttcta atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg
    19681 atttctttcg tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct
    19741 ccttctgcca tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct
    19801 ggttctcttt ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg
    19861 ttttccaaat ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat
    19921 tcacttactg tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca
    19981 ccattgtaat cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct
    20041 tcgatatcat tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga
    20101 taatctacat cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct
    20161 gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc
    20221 tttcctgtat cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa
    20281 tggatccctt tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt
    20341 actttatcta cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact
    20401 tcatctgtca gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag
    20461 ccaaccgctt tcattttact tactagataa gccccatctt cggcagcctt tttgatatta
    20521 tcaatcacta aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc
    20581 ccttcttttt tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc
    20641 tcatatttgg ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc
    20701 gagaacatat tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct
    20761 ggttttggga tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct
    20821 actacttcca catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag
    20881 cgagaggaag tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat
    20941 gctgctgact ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc
    21001 aattcttctt gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg
    21061 atttcctcct tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag
    21121 tttttctcgg cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc
    21181 gcggatctcg tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc
    21241 tagcgccgtt gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg
    21301 gaattgcatc agatattcat tgtttgctgc accgccatct actttcaata caggaatctt
    21361 gattccagtg tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg
    21421 taaagtagct ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc
    21481 tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac
    21541 ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat
    21601 ttgaagtcca tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa
    21661 agcatagtaa acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga
    21721 taattgcggt ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt
    21781 gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat
    21841 ccctgaaatt ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc
    21901 tgagttagaa gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc
    21961 ctgatcccaa tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc
    22021 agtaacatgt gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa
    22081 catcaattcg cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg
    22141 tatcttcgtt gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc
    22201 ttgatagcct tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac
    22261 aactgcatga tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg
    22321 gttcgttatg ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc
    22381 ggcaataact gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc
    22441 ttgcgggaaa tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc
    22501 aaagataatt gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc
    22561 tgtcattgtg attccctcca ttatatgcaa acgctttctt taactactct tatcatacta
    22621 ctgttttaaa aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt
    22681 tattatgaag ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt
    22741 caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt
    22801 gtttctctgg caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat
    22861 ctcaataccg ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata
    22921 aatcggcaga ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat
    22981 catcaaaaga acattggcat actggatcat caggtaacta agctcactat cttcagctct
    23041 tttcctcggc aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga
    23101 atgcccgagc attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt
    23161 tccaacaaag aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc
    23221 ttctttgatt ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt
    23281 atctaaaaca gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc
    23341 ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt
    23401 cagttctgct gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag
    23461 ccggtccgac tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt
    23521 caatagtctt tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt
    23581 aaacgttgtt tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt
    23641 ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata
    23701 tcgtatctgt agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc
    23761 taagagctgg ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc
    23821 tgtcgctaat cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa
    23881 cttcaagctt tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa
    23941 cacaagcagc gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc
    24001 aatcaattca atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc
    24061 tctcaaacca ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat
    24121 ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct
    24181 ttgaccacac catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta
    24241 actgttcaaa cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca
    24301 tctctttttt gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt
    24361 tgagtttaac tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa
    24421 ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc
    24481 caaaccactg attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa
    24541 ttgtcttgct gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt
    24601 tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt
    24661 gagacgtttg caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt
    24721 cttcccacat ctgattgtcg gggcgagaat gattagccag caaaacaaga tcatagttct
    24781 cctttctttt ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt
    24841 cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt
    24901 caggatctag ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt
    24961 ttcttgatgc caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa
    25021 ttttctttgc attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg
    25081 ggtgaataag cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta
    25141 gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca
    25201 atcctattac tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt
    25261 tcgtgatcca agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc
    25321 cgctgggaat ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca
    25381 tcaattggcg aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa
    25441 tgttcatctg cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa
    25501 cacagtattc ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc
    25561 tctccatcat accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa
    25621 gagtatcatt tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc
    25681 cttttacgta cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt
    25741 gtgtaatgta atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca
    25801 actgcttcgc ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca
    25861 atttgaacca cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa
    25921 gcatcttctg ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta
    25981 ctgaacccag tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc
    26041 aacaatccta caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt
    26101 gaagcgactt tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca
    26161 gcataataaa ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc
    26221 aaggcccgat ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc
    26281 aaaacacttt gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg
    26341 atcccatttt ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta
    26401 ggacgctcga tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc
    26461 ccatcctctt tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg
    26521 ggcttggcaa ataaagctct ttatatgctt cttcaaaagg tttcgttaca aattcatcct
    26581 cacggttttc caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg
    26641 ctgcaagttc tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga
    26701 caccaactaa agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat
    26761 cgttgtattt atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat
    26821 ctagttttga ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg
    26881 gtgtttcata ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt
    26941 gttcaaccgc tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca
    27001 agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca
    27061 aatacgtatt tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg
    27121 tggctttgcc aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg
    27181 tttccgcatc aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata
    27241 aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt
    27301 ctccttggat tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc
    27361 tgagttcacc tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag
    27421 caccaagacc tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat
    27481 tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc
    27541 ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct
    27601 ctgattaacc tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact
    27661 aatttgctta aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg
    27721 gctaattctt tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc
    27781 cagctaccat cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac
    27841 aagcgacctt cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga
    27901 gcaatcgttg atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct
    27961 ttttctttta atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac
    28021 gcttcatcag ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat
    28081 tcatctaacg ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca
    28141 tagtatgatt tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga
    28201 tttgaaaaaa agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt
    28261 ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa
    28321 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc
    28381 ttttgaacac cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa
    28441 cagccaaaca aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag
    28501 ctgattgtac accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg
    28561 agcaatttca aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt
    28621 tggcaggaga atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc
    28681 ccttatttcc ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc
    28741 aaatgatttt ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata
    28801 gatatatcca taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc
    28861 ccacgacgta taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat
    28921 atgctgtttc aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt
    28981 gtctatagct ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa
    29041 ttgattcaaa gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg
    29101 aagatacgga tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc
    29161 tggatgagca ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta
    29221 ggctgctaag atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa
    29281 cattcgttgc gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga
    29341 agttctttgc tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag
    29401 aagctgatag ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt
    29461 tgtagctaaa gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa
    29521 atggggatgg ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt
    29581 gatctcattg aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc
    29641 agcgaaacga acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac
    29701 caaatataac ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag
    29761 acattcgtca aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc
    29821 accatgagga aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc
    29881 agcaaataaa cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg
    29941 ataataatac ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg
    30001 ccattgatct gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac
    30061 agataatcct ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc
    30121 gccccataaa aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt
    30181 cttgtttgat tatttttctt atgactagta atgatatttt tgaatctact actatttgta
    30241 acaataacca ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc
    30301 atcaataaat cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg
    30361 ccatttaaat tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg
    30421 actaccccaa ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacaggactg
    30481 tagatttttc cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat
    30541 gttggatcgt ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact
    30601 atatcctttg aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata
    30661 caggaactga tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata
    30721 taaatcggga aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca
    30781 ccagctactc caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat
    30841 ttaagagtca caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca
    30901 gataaagata aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct
    30961 cctgcttgcg cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa
    31021 gaaagattgt tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact
    31081 tgccaaaatc cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa
    31141 tatccagctg caatcagctg acttgcataa tctgcaattg gacctacaac taataaagta
    31201 attgggacca tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc
    31261 aagcttttgc gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga
    31321 ataaccgtac ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg
    31381 ctatcactat atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg
    31441 atgaaacgat ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa
    31501 tagaaaaatg catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat
    31561 aaattcaaac tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg
    31621 cctaaagtcg gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt
    31681 ttctcagttc ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat
    31741 acgtcataca cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg
    31801 actacagtga tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct
    31861 tttagggtaa agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct
    31921 ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg
    31981 ttaattaatg ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc
    32041 tccattcaat tagtacccct tataatagcg aacgaaactg tacaatagtg tgtatggttt
    32101 acttagccga gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag
    32161 tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca
    32221 agctgatact ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc
    32281 tcggcattta acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa
    32341 tctatctttt tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt
    32401 gttttctttt tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa
    32461 gattcaaact catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc
    32521 tcttgctcag tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa
    32581 agatacactg gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt
    32641 ttttttagtg tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt
    32701 tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa
    32761 ttgggacgta attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga
    32821 taacgggaga tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc
    32881 gttttcattt gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca
    32941 ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct
    33001 ttgttttttt catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct
    33061 tgtttttctt gattggaaaa agaatagggt agttctgatg ataacttttt tgtgagggtc
    33121 tctaccatcg gaataaataa tgtatacctg tttgtttttt ctaaaagttc ttctcttcga
    33181 agaatggtca aaacgagata ccgagaaaat cgtatgaagt cctctcctga aacaatataa
    33241 ttatatgata taagaacctc ttttaatgtc ttttggacag tcggcatgat attggaaaaa
    33301 attgtactga tttgttgagg caaccttgcg tgttggatca ccgtttctgt tccaacaaga
    33361 gataagaaaa ttctttttga attttcttca gcaatgattt ttagtccttt tgtcgacgaa
    33421 acttccagtt ctaatttagg attgcgctcg atccaacgca aaatcgtttt tatttgtaat
    33481 gatacggctg tttttgaaag aaagaaaatg tctgctatct tttgcatact gacataccct
    33541 ttttgaaaca ataagtacgt cacaatagtt gaagctcgga aatccatgct ggaatagatt
    33601 ttatgtttcg tcacctcttg tatgatttgc tgtttagttt cagattcaat tgattctaaa
    33661 aaataacctg aacgtttatt tgactggata atgattttct tctcgtagat agcaagcata
    33721 tttttgattt cagtttggat cgttttcttg ttggtaccta aagcttcagc taattcttcg
    33781 cttgttatcc aatgattctt atttcttaat actacaagaa tttggagttg tcgtttattt
    33841 aagtccattt gcttacacct cctctttgtt ataaacatac aggaaaaacc tctcaaatga
    33901 caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt caaggttttt
    33961 accattagac tggataattt gtttataata ttcaaaacta tcttttttca atctcctaaa
    34021 gcttccgctc ccatcatttt gtctatccac ataaataaag ccatatcgct tcctcatttc
    34081 ccctgttcct gccgatacga tatcgattgg tccccaccat aagtaaccaa tgatttggca
    34141 accatctttt attgcctcag caatttcctg aagatgtatt tttagatact cacaacgttt
    34201 tggatcgtgg attcgattca attcatctgg tgcttcatct agtccaatgc cattttcaac
    34261 tatgaataat ggtaattcat aacggtctgt caattcattc atgacataac gaaaccctag
    34321 aggatcaatt ggccaactcc aaggttcggg agatttttcc ttcaaaaagg gattatcgaa
    34381 accatttgct ccgccagtat ctacgcgcat ttctgcttct tttttataga cagcgctacg
    34441 ataataacta aatgcaatat aatctgccgt gttagcttgc aagcactgtt cgtctccatc
    34501 taacattact ggtgcacagt cttcttcctt ccatgtcctg taaatataag ctggataatg
    34561 ccctcggcac atgacatcgg agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa
    34621 tacattctct ggatcacagt catatgcata agtcgctaat gcagaaaagc tacacataat
    34681 acccatctta gcatttggat cgattttctt taataattga gtagcacggg cgttcgctag
    34741 aaacatatga tgacaggctt gataaatcac tttttttgtt agtgtagcaa tgggttcaag
    34801 tttatctcta gcagggaatg taaccaatcc agcagctgca taaggcatac gaaaagcatt
    34861 attgatctca ttaaaggtca tccaatattt cactttccct tggtagcgtt caaagacggt
    34921 ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt tgccaccccc catactctcc
    34981 taccaatgct aagggggttt cataatgact aagagtaatt actggctcga tatcatatcg
    35041 atttaatgta ttgattagtc tgtcataaaa tacgagacca gcctcatttg gcttctgttc
    35101 gtctcctctt ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa
    35161 ctccttcagt aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga
    35221 aagataatgg agtgattcgt ctaaagcaat ctcccaaatc tttttttctt cattccactg
    35281 aatactagga tgctggttca tgatcccgat aagcgtatct gtcacattag gtaatttgcc
    35341 gtcttccaac catgcacctt cacattgatt agcagctact gctccgcccc ataaaaaatt
    35401 ttctggaaat atttcctgat ccataactat ctctcctcgc tatcaacaat ttcttacttg
    35461 aagtatagct cgattcatca aaacaagata ttctaactat ttcggcagaa gagaagaaaa
    35521 aaattgaatg ctactcttca tttttaatat ttatatataa caaatacata agacgatccc
    35581 cgaaagaaaa cactcgttaa ccatctaagc aaacgattta ttttacttcc aacacaaatg
    35641 cttcataagg tcgtaaatca actttgccgt ctggtaactg tttcagagga taatttgaca
    35701 caataaccct taaagcttca cgtggaacag tgaacgtctg cttttcactg gacatattac
    35761 aaataacgag ccattgctgg tcttcatata tgcgagaata ggcaaagaca tgctcaggcg
    35821 tcttttccac taattcatag tctccccaga cgatgatgtc atgttttcta cgcaaatcga
    35881 tcaatttctt gtacatataa aaaacagaat cttgatctgc taacgcttct tctgcattga
    35941 tttctttata atttggattg actcgaagcc atggaattcc agtagtaaac ccagcatgtt
    36001 cacgggcgtt ccattgcata ggcgtcctag cattgtctct tcctttcgca ttgattgatt
    36061 ccaagatttc ctcttttgta aatccagaag aaatcctttc gttatacata ttgattgtct
    36121 caatatcttt tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct aactcttctc
    36181 cttgatagat ataaggggtg cctttcatca tatggagcaa gatagcaaac atcttggcac
    36241 tttcttttcg atacacatcg tcatttcccc aacgagaaac aattcgcgga agatcgtgat
    36301 tattccaaaa taaactattc catccttcat cacctaaaga tgtttgccat ttggataata
    36361 cttttttcag ttcagagata ctcaagggtt tcaaatccca tttgtctttg ccttcttgct
    36421 gatctaatcc tatgtgttcg aattggaaga ccatggataa ttcatttctt ttaggattgg
    36481 aataaagttt agcgatttcg gtagttgctc cccatgtttc gccaacagtc atcagatttt
    36541 tgtcaccaaa agttgcttga ttcatttcct gaagatactc atgcagttta ggtccattac
    36601 ttgtgatttc tttatccggt tctttgccaa tcatatcgat cacgtccata cgaaagcccc
    36661 caataccttt atcgatccag aaattcatca tttgataaat ctcttggcga acctgtttat
    36721 ttttccaatt caaatcaggt tgacgcttgc tatacaagtg cagaaaatat tgccccgttt
    36781 tttcatccaa ctgccaagca gaaccactga atattgagcg caatctatta ggctcttttc
    36841 cattgaccgg atcacgccaa atataatagt cccgataagg attttcttta tttttacggg
    36901 cctcaatgaa ccacggatgt tcatcagaag tatgattcac caccagatcc atgatgatac
    36961 ggatatttct tttctttgct tcttcaatca gttcttccat ttcttccatc gtaccgaatt
    37021 ctgccatgat tgcttgataa ttactgatat catacccatt gtcatcattc ggagactcgt
    37081 atactggaga aagccaaatt gcatcaatac ctaaatacgc cagataatcc aacctggaaa
    37141 taatcccccg caaatctcca ataccatctt gatttgaatc ttgaaaactt cttggataga
    37201 tttgataaac aactgaatgg tgccaccatt tttgttccat gattttcctc ctataaatgg
    37261 atgtttctat tttttgctta atcatttatt ttagtctctc tcactaaaaa ttgtaacgct
    37321 ttcaaatagc tattgcacct tgttatcggt aacagaataa aacaaaaaaa cagctgagga
    37381 aattccctca actgtttttt gaaaggattg atttatgcag ggaagaatgc tgatttcaca
    37441 acttctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaacggcaaa
    37501 atatgaggaa ctattttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    37561 aacctcttta taaacggtgt tcactcagct gtccctcggt attagctcaa aaaacggcaa
    37621 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37681 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37741 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37801 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37861 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37921 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37981 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    38041 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    38101 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38161 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38221 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38281 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38341 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38401 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38461 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38521 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38581 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38641 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38701 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38761 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38821 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38881 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38941 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    39001 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    39061 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39121 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39181 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39241 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39301 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39361 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39421 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39481 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39541 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39601 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39661 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39721 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39781 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39841 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39901 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39961 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaaa
    40021 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    40081 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    40141 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    40201 accgctgctt gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag
    40261 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    40321 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    40381 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    40441 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    40501 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    40561 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    40621 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    40681 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    40741 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    40801 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    40861 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    40921 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    40981 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    41041 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    41101 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    41161 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    41221 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    41281 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    41341 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    41401 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    41461 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    41521 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    41581 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    41641 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    41701 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    41761 tttcagccat ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg
    41821 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    41881 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    41941 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    42001 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    42061 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    42121 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    42181 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    42241 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    42301 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    42361 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    42421 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    42481 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    42541 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    42601 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    42661 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    42721 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    42781 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    42841 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    42901 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    42961 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    43021 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    43081 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    43141 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    43201 cgatcacgtt tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg
    43261 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    43321 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    43381 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    43441 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    43501 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    43561 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    43621 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    43681 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    43741 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    43801 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    43861 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    43921 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    43981 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    44041 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    44101 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    44161 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    44221 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    44281 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    44341 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    44401 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    44461 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    44521 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    44581 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    44641 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    44701 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    44761 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    44821 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    44881 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    44941 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat
    45001 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    45061 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    45121 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    45181 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    45241 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    45301 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    45361 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    45421 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    45481 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    45541 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    45601 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    45661 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    45721 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    45781 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    45841 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    45901 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    45961 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    46021 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    46081 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    46141 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    46201 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    46261 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    46321 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    46381 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    46441 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    46501 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    46561 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    46621 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    46681 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    46741 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    46801 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    46861 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    46921 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    46981 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    47041 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    47101 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    47161 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    47221 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    47281 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    47341 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    47401 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    47461 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    47521 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    47581 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    47641 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    47701 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    47761 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    47821 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    47881 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    47941 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    48001 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    48061 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    48121 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    48181 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    48241 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    48301 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    48361 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    48421 agcttgcttt tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc
    48481 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    48541 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    48601 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    48661 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    48721 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    48781 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    48841 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    48901 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    48961 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    49021 aaatcgtgcc ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc
    49081 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    49141 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    49201 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    49261 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    49321 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    49381 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    49441 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    49501 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    49561 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    49621 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    49681 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    49741 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    49801 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    49861 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    49921 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    49981 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    50041 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    50101 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    50161 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    50221 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    50281 gtttctgtac atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat
    50341 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    50401 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    50461 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    50521 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    50581 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    50641 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    50701 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    50761 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    50821 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    50881 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    50941 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    51001 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    51061 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    51121 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    51181 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    51241 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    51301 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    51361 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    51421 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    51481 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    51541 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    51601 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    51661 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    51721 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    51781 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    51841 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    51901 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    51961 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    52021 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    52081 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    52141 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    52201 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    52261 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    52321 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    52381 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    52441 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    52501 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    52561 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    52621 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    52681 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    52741 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    52801 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    52861 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    52921 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    52981 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    53041 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    53101 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    53161 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    53221 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    53281 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    53341 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    53401 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    53461 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    53521 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    53581 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    53641 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    53701 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    53761 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    53821 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    53881 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    53941 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    54001 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    54061 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    54121 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    54181 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    54241 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    54301 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    54361 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    54421 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    54481 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    54541 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    54601 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    54661 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    54721 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    54781 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    54841 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    54901 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    54961 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    55021 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    55081 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    55141 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    55201 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    55261 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    55321 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    55381 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    55441 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    55501 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    55561 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    55621 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    55681 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    55741 gttgacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    55801 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    55861 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    55921 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    55981 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    56041 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    56101 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    56161 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    56221 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    56281 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    56341 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    56401 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    56461 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    56521 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    56581 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    56641 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    56701 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    56761 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    56821 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    56881 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    56941 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    57001 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    57061 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    57121 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    57181 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    57241 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    57301 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    57361 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    57421 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    57481 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    57541 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    57601 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    57661 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    57721 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    57781 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    57841 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    57901 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    57961 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    58021 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    58081 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    58141 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa
    58201 aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca
    58261 gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta
    58321 aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta
    58381 gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt
    58441 tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa
    58501 tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata
    58561 ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg
    58621 accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta
    58681 aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc
    58741 gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag
    58801 aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc
    58861 aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt
    58921 attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga
    58981 aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa
    59041 cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt
    59101 cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac
    59161 aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg
    59221 aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca
    59281 tcatcagcga tcaagcagga attagtttgc aaaaccagca aaatttatta acaaatgacc
    59341 tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta
    59401 caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga
    59461 aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga
    59521 cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat
    59581 tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc
    59641 attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga
    59701 ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag
    59761 attcttctat ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat
    59821 tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct
    59881 ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc
    59941 attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc
    60001 ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc
    60061 atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat
    60121 atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa
    60181 aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac
    60241 aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac
    60301 cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct
    60361 tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga
    60421 tttccaattt ccctctagat tctctagata tccctattgt atacatttct tctattccaa
    60481 cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc
    60541 tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct
    60601 ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt
    60661 gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc
    60721 ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct
    60781 tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag
    60841 acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat
    60901 aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca
    60961 gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac
    61021 tcgaacccta gttcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    61081 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    61141 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    61201 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    61261 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    61321 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    61381 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    61441 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    61501 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    61561 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    61621 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    61681 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    61741 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    61801 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    61861 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    61921 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    61981 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    62041 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    62101 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    62161 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    62221 atggtcaaaa cagctggcaa tcctttgggt accattgaca ccaccaaagc aatcgtagcc
    62281 gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    62341 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    62401 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    62461 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    62521 gccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    62581 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    62641 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    62701 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    62761 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    62821 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    62881 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    62941 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    63001 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    63061 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    63121 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    63181 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    63241 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    63301 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    63361 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    63421 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    63481 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    63541 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    63601 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    63661 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    63721 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    63781 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    63841 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    63901 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    63961 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    64021 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    64081 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    64141 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    64201 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    64261 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    64321 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    64381 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    64441 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    64501 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    64561 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    64621 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    64681 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    64741 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    64801 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    64861 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    64921 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    64981 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    65041 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    65101 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    65161 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    65221 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    65281 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    65341 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    65401 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    65461 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    65521 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    65581 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    65641 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    65701 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    65761 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    65821 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    65881 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    65941 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    66001 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    66061 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    66121 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    66181 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    66241 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    66301 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    66361 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    66421 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    66481 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    66541 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    66601 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    66661 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    66721 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    66781 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    66841 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    66901 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    66961 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    67021 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    67081 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    67141 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    67201 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    67261 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    67321 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    67381 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    67441 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    67501 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    67561 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    67621 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    67681 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    67741 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    67801 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    67861 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    67921 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    67981 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    68041 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    68101 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    68161 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga aatttttttt
    68221 tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg
    68281 ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac
    68341 ctaaaaaaag cgaacaaaca aataaaatga ctgtcctt
//
LOCUS       DAJSNV010000003        68087 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSNV010000003 DAJSNV010000000
VERSION     DAJSNV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.003"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            129..728
                     /locus_tag="ON516_000141"
     CDS             129..728
                     /locus_tag="ON516_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3343660.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(797..2097)
                     /locus_tag="ON516_000142"
                     /pseudo
     CDS             complement(797..2097)
                     /locus_tag="ON516_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     misc_binding    2360..2565
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2677..3933
                     /locus_tag="ON516_000143"
     CDS             2677..3933
                     /locus_tag="ON516_000143"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3343661.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            4224..6101
                     /gene="tdc"
                     /locus_tag="ON516_000144"
     CDS             4224..6101
                     /gene="tdc"
                     /locus_tag="ON516_000144"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3343662.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            6344..7765
                     /locus_tag="ON516_000145"
     CDS             6344..7765
                     /locus_tag="ON516_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3343663.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            7874..9241
                     /gene="nhaC"
                     /locus_tag="ON516_000146"
     CDS             7874..9241
                     /gene="nhaC"
                     /locus_tag="ON516_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3343664.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(9522..10541)
                     /locus_tag="ON516_000147"
     CDS             complement(9522..10541)
                     /locus_tag="ON516_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3343665.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10563..11570)
                     /locus_tag="ON516_000148"
     CDS             complement(10563..11570)
                     /locus_tag="ON516_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3343666.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(11563..12228)
                     /gene="pgmB"
                     /locus_tag="ON516_000149"
     CDS             complement(11563..12228)
                     /gene="pgmB"
                     /locus_tag="ON516_000149"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3343667.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(12221..14515)
                     /locus_tag="ON516_000150"
     CDS             complement(12221..14515)
                     /locus_tag="ON516_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3343668.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14775..16940
                     /locus_tag="ON516_000151"
     CDS             14775..16940
                     /locus_tag="ON516_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3343669.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            17244..18077
                     /locus_tag="ON516_000152"
     CDS             17244..18077
                     /locus_tag="ON516_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3343670.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(18179..19879)
                     /locus_tag="ON516_000153"
     CDS             complement(18179..19879)
                     /locus_tag="ON516_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343671.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            20386..21546
                     /locus_tag="ON516_000154"
     CDS             20386..21546
                     /locus_tag="ON516_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3343672.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            21562..22005
                     /locus_tag="ON516_000155"
     CDS             21562..22005
                     /locus_tag="ON516_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3343673.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(22488..23018)
                     /locus_tag="ON516_000156"
     CDS             complement(22488..23018)
                     /locus_tag="ON516_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3343674.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            23123..23674
                     /locus_tag="ON516_000157"
     CDS             23123..23674
                     /locus_tag="ON516_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3343675.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(23827..24300)
                     /locus_tag="ON516_000158"
     CDS             complement(23827..24300)
                     /locus_tag="ON516_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3343676.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            24438..25910
                     /locus_tag="ON516_000159"
     CDS             24438..25910
                     /locus_tag="ON516_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3343677.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            25907..26617
                     /locus_tag="ON516_000160"
     CDS             25907..26617
                     /locus_tag="ON516_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3343678.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            26969..27253
                     /locus_tag="ON516_000161"
     CDS             26969..27253
                     /locus_tag="ON516_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3343679.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(27534..27953)
                     /locus_tag="ON516_000162"
     CDS             complement(27534..27953)
                     /locus_tag="ON516_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3343680.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(27955..28539)
                     /locus_tag="ON516_000163"
     CDS             complement(27955..28539)
                     /locus_tag="ON516_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3343681.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(28586..30061)
                     /locus_tag="ON516_000164"
     CDS             complement(28586..30061)
                     /locus_tag="ON516_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3343682.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            30242..30553
                     /locus_tag="ON516_000165"
     CDS             30242..30553
                     /locus_tag="ON516_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343683.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(30668..33004)
                     /locus_tag="ON516_000166"
     CDS             complement(30668..33004)
                     /locus_tag="ON516_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3343684.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            33146..33814
                     /locus_tag="ON516_000167"
     CDS             33146..33814
                     /locus_tag="ON516_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3343685.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            33817..34860
                     /locus_tag="ON516_000168"
     CDS             33817..34860
                     /locus_tag="ON516_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3343686.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35097..35978)
                     /locus_tag="ON516_000169"
     CDS             complement(35097..35978)
                     /locus_tag="ON516_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3343687.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            36137..36865
                     /locus_tag="ON516_000170"
     CDS             36137..36865
                     /locus_tag="ON516_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3343688.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            37021..37338
                     /locus_tag="ON516_000171"
     CDS             37021..37338
                     /locus_tag="ON516_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3343689.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            37331..37939
                     /locus_tag="ON516_000172"
     CDS             37331..37939
                     /locus_tag="ON516_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3343690.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            37994..38785
                     /locus_tag="ON516_000173"
     CDS             37994..38785
                     /locus_tag="ON516_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3343691.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            38923..39411
                     /locus_tag="ON516_000174"
     CDS             38923..39411
                     /locus_tag="ON516_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3343692.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            39462..40334
                     /locus_tag="ON516_000175"
     CDS             39462..40334
                     /locus_tag="ON516_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3343693.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            40510..40872
                     /locus_tag="ON516_000176"
     CDS             40510..40872
                     /locus_tag="ON516_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3343694.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            40881..41633
                     /locus_tag="ON516_000177"
     CDS             40881..41633
                     /locus_tag="ON516_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343695.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            41647..43449
                     /locus_tag="ON516_000178"
     CDS             41647..43449
                     /locus_tag="ON516_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343696.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            43726..44766
                     /locus_tag="ON516_000179"
     CDS             43726..44766
                     /locus_tag="ON516_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3343697.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(44818..46935)
                     /locus_tag="ON516_000180"
     CDS             complement(44818..46935)
                     /locus_tag="ON516_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3343698.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(47292..48665)
                     /locus_tag="ON516_000181"
     CDS             complement(47292..48665)
                     /locus_tag="ON516_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3343699.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(48665..49060)
                     /gene="rbsD"
                     /locus_tag="ON516_000182"
     CDS             complement(48665..49060)
                     /gene="rbsD"
                     /locus_tag="ON516_000182"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3343700.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(49088..50005)
                     /gene="rbsK"
                     /locus_tag="ON516_000183"
     CDS             complement(49088..50005)
                     /gene="rbsK"
                     /locus_tag="ON516_000183"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3343701.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(50113..51126)
                     /locus_tag="ON516_000184"
     CDS             complement(50113..51126)
                     /locus_tag="ON516_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3343702.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(51207..51806)
                     /locus_tag="ON516_000185"
     CDS             complement(51207..51806)
                     /locus_tag="ON516_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3343703.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            51992..52693
                     /locus_tag="ON516_000186"
     CDS             51992..52693
                     /locus_tag="ON516_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343704.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            52703..55414
                     /locus_tag="ON516_000187"
     CDS             52703..55414
                     /locus_tag="ON516_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343705.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            55811..57439
                     /gene="treC"
                     /locus_tag="ON516_000188"
     CDS             55811..57439
                     /gene="treC"
                     /locus_tag="ON516_000188"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3343706.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(57499..58680)
                     /locus_tag="ON516_000189"
     CDS             complement(57499..58680)
                     /locus_tag="ON516_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3343707.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            58932..60455
                     /locus_tag="ON516_000190"
     CDS             58932..60455
                     /locus_tag="ON516_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3343708.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(60524..61252)
                     /locus_tag="ON516_000191"
     CDS             complement(60524..61252)
                     /locus_tag="ON516_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3343709.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(61265..62545)
                     /locus_tag="ON516_000192"
     CDS             complement(61265..62545)
                     /locus_tag="ON516_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3343710.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(62686..63288)
                     /locus_tag="ON516_000193"
     CDS             complement(62686..63288)
                     /locus_tag="ON516_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3343711.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            63533..63739
                     /locus_tag="ON516_000194"
     CDS             63533..63739
                     /locus_tag="ON516_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343712.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            64051..64770
                     /locus_tag="ON516_000195"
     CDS             64051..64770
                     /locus_tag="ON516_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3343713.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(64851..65534)
                     /locus_tag="ON516_000196"
     CDS             complement(64851..65534)
                     /locus_tag="ON516_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3343714.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            65752..67938
                     /locus_tag="ON516_000197"
     CDS             65752..67938
                     /locus_tag="ON516_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3343715.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        21753 a        12116 c        13804 g        20414 t
ORIGIN      
        1 tctgtcaatg aattcataaa caaggaattg ctagcttgat actttctcaa tagatagtta
       61 taataaacct cagaataaat ttgaactata catattataa aagtttaaaa ttacgggagg
      121 ctctacaaat gaacggtttt gaaaagagag cacaagagaa aaagaaagag attttacaag
      181 cagcttttga cttgatgaac acagacatag gaaccgctgg agtgacaatg gataaggtag
      241 ctgagaacgc tcaggtaagt aaagcgacga tttttaaata ttttggcagc aaggaagggc
      301 tgatttatga agtatttttt gcatttatga atgatttagg gcaagaagcg aaagcgttga
      361 ttgcggaaaa tcttccattt gaagaaacac tgattgccat gagtgaaaac aaaatccgtt
      421 atctcaatcg agtgaacaaa caattttata tcgatttgat gacatactat acaaaaaaag
      481 aaaaaaaaga attttctgac gcgatggatg cgtatacgca agaaagtttc agtatcatgc
      541 ttgatctttt ccatcgaggg cggaaagaag gaaaagtgga tttgaaatat tcggatgagt
      601 tcttgatcct ctattttcaa gcaatggtag aaggaatttc taagccggag atatacgcaa
      661 aactgcttcc atatacagca gaatggacag aagtcctgat caagggaatc gcaccgaaga
      721 agaaataaag cataaaaaag ccgaaactga cgataaaatg tcagttcggc ttttttggtt
      781 cataaatgac gtaaaatcaa gcagattcgc gtgcttcttt gtcatagaca tcatggacat
      841 tgatgaatgg atcaagaata ggattttttc cgtctaaatc agattgaaaa cgaatgccat
      901 cgatattgat ccgagcattt cgatagaaat ccgtacctaa ttgtccccaa gctggtgtag
      961 aagcatcgac attacagaaa atatcgaaac cttgactctt caagtaagca aatttttgat
     1021 tttctgctga ataaggctgc cagtcgctga tgtctgcacc aaatggataa atcagaatat
     1081 ttgtttttcc taggataggt gcaacttctt gctgccacaa ttcattgtct ttttttacat
     1141 cttctagtga agcttgagtc atattcaagt gaccccatgt atgggaagca aagctccagc
     1201 cgtctttctt caactgttcc gccacttttt tagcttcttt aatctctttt tttgtttttt
     1261 cattgtctcc atattgagat tttgatgttc gataacctaa aactccgttg taacctgtca
     1321 gggccaaggt accttttgcg cctcgataag aaaaatcagg atgtttcttg acaaaggcat
     1381 cgatcagagg aaccatgtca aagtcaccga tttcttcttt atctttctct ttatagacat
     1441 ttttgatttc attgttattt gatacgacca atcggctagg aaaaccggaa ttatccatat
     1501 attcataata gctgacatca tcttgtgaaa gaaccaatgg tttttttcct tcaggtagcg
     1561 caagcccgtt aaaggaaaga tttccttgtt cgtctttttg gatcaaatca ttcaaattaa
     1621 ctaaaacaaa atcattttga taaagttgtt cgatcgaacg gttgaattct tcaacagtca
     1681 ccatataatc tttgtatcct tgtgcctgtt gagagtggaa ggctttttgc gggtccacga
     1741 tcaagctgtg gaaaaagaca tgagagatct gagtaggatc agaccagtcg atcagttgtt
     1801 ctttctcctt ttttagtgtt tgaagaagct gttgtgtttc agtagatccg ttcttactat
     1861 ctttttccaa taacgcaatc gcttggtcat aatcgtagct ttgagccaga ttttttgctt
     1921 gagtaactag ttgttgctta tcatctgtcg gttcttttgc tttttctgtt attttttttc
     1981 aggaatgatt ggttcttttt ttgtagatga tcggatgaca aaaaacaaac ttagaattaa
     2041 aataacgata gttaaagcag aagtggctcc taaaataatt ttttgctttt tttccatata
     2101 cctcgtccct tgctttttca tttttttact tgtcccattc cttattgtaa cgaagaaaga
     2161 ataagaggca aagtaaagtg acttctcgat aaattgcaga aaaaatacag aaaaagttat
     2221 ttcttttaac aataattgaa aataaaatgt tcggaattag agcggattca aaaaagaaaa
     2281 aatgaggaat ttaaaaaaaa tatccttttt ctgttgacaa agaaaaagtt gcgctctatg
     2341 atagacacat attaataata acaagacgaa gatcgaaacg aagtagttgt taaagccaat
     2401 gtttcagaaa gctggtggtc gctgcaaacc agtacatgct ttaaagcgaa ttacatttca
     2461 ggagtctttg gcagtgattg ccacgcgaca gcgttaaatg ttcgagaggt atgttggata
     2521 tctagacata caaattgggt ggtaccgcga gatttcgtcc cttggcttat atgccaaggg
     2581 acttttttta tttcctatcc tatcctgaag aaataattaa ttacatatgc tagcaggttc
     2641 ctgctttcat aaaaaaacta cacggaggtc agtaaaatga atattattga tgaattaact
     2701 tggcgcgacg ccattaatca gcaaacaaac gaagaaagac ttcgcgagct cgttgaagaa
     2761 aagagcattt cactttattg cggcgttgat ccaactggtg attcgatgca tatcggacat
     2821 ttgattccat ttatgatgat gaaaagattc caattggcag gccatcatcc atatattctg
     2881 attggcggcg gaacaggaac gatcggcgac ccaagcggcc gcaaaacaga acgtgtgttg
     2941 caaacgatgg aacaagtgca acataacgtg gacgcacttt ccaatcaaat gagaaaattg
     3001 tttggtaaag atgcaaatat cacatttgtg aacaactacg actggttgtc aaaaatctct
     3061 ttacttgaat ttttgagaga ctacggtaaa aacttcaaca tcaacacgat gttagcaaaa
     3121 gatatcgttg ccagccgttt ggaagtagga atctcattta cagagtttac ttaccaaatc
     3181 ctgcaatcta tcgacttttt gcatctgcat aaaacatacg atgtgcaatt gcaaatcggt
     3241 ggagcagacc aatggggaaa tatcacagct ggattagacc taatccgaaa attagaagga
     3301 ccagaagcag aagcgttcgg tttaacgatc ccattgatgc tgaaggctga cggaacgaaa
     3361 ttcggaaaaa ctgcaggcgg tgccgtttgg cttgatccga agaaaacttc accatttgag
     3421 ttctaccaat tctggttgaa tcaagatgat cgcgatgtag tgaaatactt gaaattcttt
     3481 actttcttgt ctcaagaaga aatcgaagac ttagcgaaaa aagtagagac agaacctgag
     3541 aaacgtgaag cacaacgccg tttggcagaa gaagttacaa gatttgtgca tagtgaagaa
     3601 gacttgaaag aagcgcaaaa aataacgcaa gcattgttct ctggaaatat taaagaacta
     3661 aatgcggaag aaatcgcgca aggattcggt aaaatgccga acgttgaaat ttcaagcaca
     3721 cctgaaaata tcgtggaact gcttgtttcc acaaaaattg aaccatctaa acgtcaagca
     3781 cgtgaagatg tttcaaacgg agctataagt attaacggtg accgcgttac cgatttaaat
     3841 tttgtcataa atccatccga tgaattcgat ggtaagtttg tggttattcg aaaaggtaag
     3901 aaaaattact ttttggccaa agtaattgat tagaatttag taagttttta ccttaaaaaa
     3961 gggaaatact attacaacat tccaattttg aaacccatcc taaaatagaa cgctggttgt
     4021 cgttaataca atctttgcga gggagtttac atttgtaaat tccctcgttt tttttaatta
     4081 tttcaaaagt gtgaacaatt cggataattg atacatatta aaatatgatt aaaaatcagg
     4141 ccaaaactta gtttacagaa tagaagaaca ggtgtaatgt agggctcgac aaaatttaaa
     4201 tattttttta ggagggtttc actatgagtg aatcattgtc gaaagatcta aacctaaatg
     4261 ccctattcat tggggacaaa gctgagaacg gccaaatcta taaagctttg ttgaatgaat
     4321 tggtggatga acatttaggc tggcgtcaaa actacatgcc tcaagacatg cctattatta
     4381 cgccagaaga aaaaagcagt gctagttttg aacacactgt gaataaaaca aaggatgttc
     4441 tatcagaaat ttcagcgcgg atgcgtaccc attccgttcc ttggcataat gccggacgtt
     4501 actggggtca tatgaactct gaaacattga tgccatcttt actagcgtac aattttgcta
     4561 tgctatggaa tggtaataac gttgcctatg aatcttctcc tgcaacaagc caaatggaag
     4621 aagaagtagg aatggaattt gcaaaattaa tgagctataa agatggctgg ggccacatcg
     4681 ttgctgatgg ttctttagca aacttagaag gactttggta tgcccgcaac atcaaatcat
     4741 tgccgcttgc aatgaaagaa gtgacacctg aactagttgc tggaaaaagc gattgggaat
     4801 taatgaactt gtcaacagaa gaaatcatga atctgttaga cagcgtccca gaaaaaatcg
     4861 acgaaatcaa agctcactca gcacgtagcg gaaaacattt agaaaaatta ggaaaatggt
     4921 tggttccaca aactaaacac tattcatggc taaaagctgc cgacatcatc ggtatcggtt
     4981 tagaccaagt gattccagtg ccagttgacc acaactatcg catggacatc aacgaacttg
     5041 aaaaaatcgt tcgtggtcta gcagctgaaa aaacaccaat cctaggtgta gtcggtgttg
     5101 taggatcaac agaagaagga gcaatcgacg gtatcgataa aatcgttgca ttgcgtcgcg
     5161 tattagaaaa agacggtatt tacttctact tacacgtaga tgctgcttat ggcggatacg
     5221 gccgtgcgat tttcttagac gaagacaaca acttcattcc atttgaagat ttaaaagatg
     5281 ttcattacaa atacaatgtc tttacagaaa acaaagatta tatcttagaa gaagtacaca
     5341 gtgcatataa agcaatcgaa gaagcagaat ctgtaacgat tgacccacat aaaatgggct
     5401 atgttccata ttctgccggt ggtatcgtca tcaaagatat ccgcatgaga gacgttatct
     5461 cttactttgc gacatatgta tttgaaaaag gcgcagatat cccagcatta cttggggctt
     5521 atatcttgga aggttcaaaa gcaggggcaa cagcagccag cgtttgggca gcacaccatg
     5581 tcttaccttt gaatgtcaca ggatatggta aattaatggg tgcatcaatc gaaggtgcac
     5641 atcgtttcta caacttcttg aatgacttgt catttaaagt aggagacaaa gaaatcgaag
     5701 ttcatccatt aacttatcca gatttcaata tggtagacta tgtattcaaa gaaaaaggca
     5761 acgatgactt agttgcaatg aataaattga accatgacgt atatgattat tcttcatacg
     5821 tgaaaggaag catttacggt aatgagttct taacttcaca tactgacttt gctatcccag
     5881 actatggcaa cagcccatta caatttgtga accaactagg attctcagat gaagaatgga
     5941 accgtgccgg aaaagtaact gtattgcgtg ccagcgtaat gacaccatac atgaacaaag
     6001 aagaacactt tgaagaatat gctgaaaaaa tcaaagcagc tcttcaagaa aaattggaaa
     6061 aaatctacgc agaccaatta ttggcaagcg aagcaaaata agcaaggtaa tcatgttaga
     6121 aaacagcttg aatactaact gtttctgacg catgtttgga tctgaagagc aaggggagac
     6181 ctaagctctt cgatcccttt gttttgaggt tgtctttgaa gaaacccttc aagcaataaa
     6241 ctcaaagaat cccctcaaat attcgctgaa ttgtttcgtt tattgctgaa actacttcaa
     6301 agacaccgtt attttatcca aaaaatcaag gagagtgtca acggtgagta aatctgaagt
     6361 aagtaaaatg ggtcttggga cctttatcgg tctgacaatg gcactgtgtg caacggttcg
     6421 gagtattcct acgttagccg cagtcggttg gacattgatt ttttattcaa tttttgcagt
     6481 tttgtttttt gccggtccga tatcaatggt ttccggtgag ctttccacga tgcttcctca
     6541 agaaggcggt cctcagttat gggtaaaaac agcactgggc agcaaatggg gatttgtcgt
     6601 tgcatggcta ttatgggtgc aaatgttccc aggtatggta atggtcgctt ctactttagg
     6661 accattgtta ggaaacacat ttggtaatgt agaacttggc aataatcact ggttcgtttt
     6721 aggttgtatt ttagttatct actggatcat cacgatcttg aacttgaaat tcgatatggc
     6781 aaaagtcggc gggaacatcg gtgtatggct aggggtatat atcccagtag tcatcatgtt
     6841 cgttttaggt gttttggcag ccttcaaagt cggactagtc tcaaacggct atctaggtga
     6901 cttttcatgg tcaaaagctt tccctgactt ggaacatatt gattcattga aatacttagc
     6961 tggtatcaca tttatttttg ttgggatcga aatgagttct gtttacatgc ctcgtttgaa
     7021 agacgcaaca aaaaattata caaaaggcgt atttatcgct ttgatcggat tagttttatt
     7081 gaatgtcatc aatgcgatgc ttgtagcaaa tgtcgtacca gatggaaaaa tggaattagc
     7141 aaatatcacg cagcctatct tgattgactg tcaaatccta ggcttaccag aagtgatcgg
     7201 aaatatcttc agcttcatgg tatttattgg tgtgctttta caattatcag cttgggttac
     7261 tggaccatca aaaacaatca tccaagtagc tcgtgaagga ttccttccac caaaattcgg
     7321 attccataaa gaaaacaaat acggcgtatc tagaaatgtc gttctaacac aatcaatcgt
     7381 tatttcatta tttgctttgc tttatggtgt aatggatgac gtaagtgctg tattcttgac
     7441 acttacaaat gctacaactg ttatttattg tatcgtgtac attttgattg ctgtttcatt
     7501 gcttaaaatg cgtaagaaac atccagaatt cgaacgtcct taccgaattg gtaaaaacgg
     7561 aaacggatta gcttgggtag tcagctgcat gctgatcttc tctatcatcg ttgtcgtatt
     7621 tgctacatta ggtacagcga cattgagtga tgcattatta gtagcagcta tcacagtggt
     7681 catgttcgtt attccattga tcatcaatca cttcaagaaa gattcttggg ggattgaggt
     7741 agaaaaaagt ttagaagaaa aataaaaaaa taagtctttc agccgacgtt ttgtcgctga
     7801 tttagtagga ggtcctgcca agtgaatagt tttttcatgg cagggccttt tcatttgaaa
     7861 taggagatcg acgatgaata aaaatatttc tttaaaagaa agtatcgctg tattgatcgt
     7921 gttgttggca ctcctaggag tactgatcat cggattccag ctctcgccac atatcccgat
     7981 ccttgcagct ttcatgtgtt tgttgtttta tggcagattt aaaaagttca catgggatga
     8041 gatccatgac gggatcatca aaggaatcac tccagggatc attccaatcc tgatttttct
     8101 tttgattggt gtcttagttg cttcatggat tttatctgga accatcccta cgattatggt
     8161 ttatggattt aaacttgttt ctgtgcgttt ctttttaccg acagcatttc ttgtttgtgc
     8221 aattgtcgga gtgactgtcg gcagttcatt tacaacgatt tcgacgattg gaattgcttt
     8281 tttggggatc ggtcatttac ttggattcaa tgatgcaatg actacaggcg cagtcgtttc
     8341 cggtgccttt ttagggaata actgctcgcc tttatcagat acgaccaact tggcagcggg
     8401 tattggagga gttaatctat tcgaacatat cgctggtatg cgttatacgg atttcccagc
     8461 attcgtcctt tcgttgattt tttatacgat attaggacat aacagtcatt cagccgatct
     8521 agctagtatc aatgaaatga tccaaaacat gaaagcgagc ttttggatct ctccttggac
     8581 attgattcca ctagttattt tatttggggg ggcaatcaaa aaaattccgg ctatccctac
     8641 attactagcg ggatcgacag ctgctctcgt tctttctttt atccatgaga aaagtttgac
     8701 ggtcggaaaa gcagcagaca tcttgatgaa tggttatgtc gcaaatacac ctgatccaaa
     8761 gctagatgaa ctgctttctc gaggcgggat catgagtatg ttaggttctg ctagtttgat
     8821 tttacttgca ttggcattag gtgggttact gatcaaatat ctgatcgttg ataccatcgt
     8881 tcaagaatta aaagaaaaga tggaccgacc ttctcgttta gtcgggttta ctgccttgag
     8941 ctgtatcgga atcaatctga tcgttggaga acagtatctg tccatcattc ttccaggaga
     9001 aacattcaag cgttcttttg atgacgttgg cttatcaaaa acctatttga cacgaacact
     9061 agcggattca ggtgcgaccg tcaattctct cgtaccgtgg ggagtcagcg gcacattcat
     9121 tatgggaacg atgaaagtta gcgcgttgca ttacttgcca ttcgcctttt tctcgattct
     9181 tgcacctgtc tttacaatat taggcggttt tttattgaat cgtcgacgtg taaaaaattg
     9241 aaggaaggaa aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaactc
     9301 aagaaaattg ctttttaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg
     9361 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     9421 gctctttttt acagataaac aatgtgttag aagtagtatc ttcttttaca taaaaagctg
     9481 accataggat aaatggtcag cgtatttagt gagtcagcta tttagtatgg gttttctaaa
     9541 ggaacgactg tctctctttt gatcaagcga tgagggatga ccaaacgtac gccactgctc
     9601 aattcttcat ggatcaattc gcgcagcttt tgcatagcga cacgtcctaa ttcggagata
     9661 tcgatatcga tacttgtaag gtaagggtgt gttaatgttg agaagatcga gttgttgaag
     9721 ctaatgacgg agagtgtctc tggaatgaca tagccataca tttgcgccaa ctgcatcgtg
     9781 cgaagggcga agatatcgtc aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt
     9841 ttctcaaagg acagataatc ctccggatgc tctagtacta cactggataa cgcaggaaga
     9901 ccagccatca tcattgcttt ttgataaccg aaatatcgtt cgaaatacaa actttcatga
     9961 gtcgtgtttg tcacaaacag gatattctta tgtccattct ggatcaaaaa ttctgttgct
    10021 tgtttaccta gaagctggtt atcattatct acataaacga tttcgttctc atgatgataa
    10081 ggctgtccga tcaaagtgaa agggatgtgg ttttggtata aataatcaat gacagtatct
    10141 tgactatctg aatacactag aataaaacca tctacttgtt tttgtaaatg catacgctgg
    10201 acgttctcta acagcaattc aaaattttta gcagtagcga ttgctgtagt catatcataa
    10261 ttccgcgctt cttcgttgat tgcttccatg atctcaagat aaaatgggtt accgatccgc
    10321 tcttttgaat caagaggggg taagattacg ccgatggcac tggaaatgcg ttttcctaga
    10381 ttgcgggcag tcatgttagg aacataacct agttcatcca taatttttcg gactttcttc
    10441 tttgttgtct cggaaatact aggatgatcg ttgatgacac gagagacggt cgaggtggca
    10501 acaccggctt ttttcgccac atcttttacg gtaatagcca tgataaaaac acctttctct
    10561 tgttattgtg cagagaaccg ataaatcgtc tgactgatcc gttcttgacc aggatggagg
    10621 ttgatcgatc caaatttggg gaaatgaacg atatcaggga tttcttgcgg ttcgattgct
    10681 aatccgtagt ttgaatgcat ctgttttcca ttgacagaga aatcagcttc gaatccagtc
    10741 gtagaaaata ataccatact tttattagaa gttgctatag tcatttggcg accagaatgc
    10801 ttctcgaata attgtaagga agttttggaa agcctaggat gatgcaaaag aaatacatca
    10861 tctagtccat ctggataaca tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg
    10921 attggcagtt tttctttttt tgtccaattt ccagtaggca gtttttcttg atctaattca
    10981 agcacaccat ctaaaaagac cgttaactga tgttcataga tgtctcttgt tccatttcct
    11041 gaaaggttga agtaggcatg gttggtcgga ttgcagatgg tttcaacaag gctactagca
    11101 gaggttgtca tctctaattc gttttttgtt aaacggtaag tgatcgtagc agtgattggt
    11161 cctggatagg tagaaaattc atcttttaag taaaagacca cacctattga tgatggagtt
    11221 ttgaaaactt ctacttccca aaattggaaa gaccagcctt tcgtgccacc gtggatatgg
    11281 tgtgatccgc agtttttctc caatctatgc tcaccccaag aagcatcccg gatccttccg
    11341 gctacaggtc cgacagttgc tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga
    11401 tcgtaagaca ataaaacatt ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc
    11461 aggcgggcac cgtaattgag aagaacgact tctaagtagt cgttcttcaa tgtgattttc
    11521 tttatttcag tctctggaac tggtacgacg gagatctctg gattatccat ggtcatgcca
    11581 cttttctttc aagtaatcga tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc
    11641 tccaaggtct tcagcacgtc ccacgccaat tggttgagcg ccacttgaga ggatggcttg
    11701 gataccggct tgcgcgtctt cgatcccgat acattcttct gcttttagtc cgacagcttt
    11761 tgctgcaagg aggaagatat ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc
    11821 aatagcatca aagtaaggtg taagacccat tttttcaagt aggaatggcc cgtttttact
    11881 tgcagaagca agagcaatct tgattttttc ttcttttaaa gaatccagta aaggcacgac
    11941 cccggggaag acatcttttg gttcgatcgt ctggatcatc tctaaataat aatcgttttt
    12001 tcttttcgct aattctgcaa attcttcctt tgaaaaagag tgttcttttc cgccataagc
    12061 taaaagcaaa gagagtgagt cttctcggct gacgcctttt agctgttcgt tgaatgtgtg
    12121 gtcgatcgta atgccgattt cctgaccaag ctttcgccaa gcacgataat ggaattcagc
    12181 agtatcggta atgacaccgt ctaaatcaaa caatacccct ttaaacattt gcttcctcct
    12241 ttcttagcgt tatttctaga cgatccgcta aagaataaga ctgttcatag acgtaaattt
    12301 ctagaggaga accttctaat aactcgaaag taacttgttc atctgtgact gagatgaaca
    12361 ataaacgtcc gcgataattg atatggaaag aataagcttt ccaagttttt ggcagaaatg
    12421 gtgcgaatct caactgatca tgggcagttt tcatttgagc aaatccttgg acaatcgaca
    12481 accagctacc agtcatggaa gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat
    12541 ccaaatccaa gcgagctgtt cgctggtaca tttctacagc tttgctttcc attcctaatt
    12601 ctgctgctaa aatcgaatgg atacttggcg aaagggaaga ctcatggaca gtcattggtt
    12661 catagaaatc aaagtttttt tgcttttctt cttttgtaaa agtatcattg aagaaataaa
    12721 tcccctgtag tacatctgcc tgcttgatga agcaagatct aaggattttg tcccaagacc
    12781 aatgctgatt cagaggaaga tcgcttgtgg gaatatcgga tacaggcatg agatctttat
    12841 ctaagaatgt gtcatgctgg acaaagattc ctaattcttc gtcaactggg aagtacatgt
    12901 tctctgcgat atctttccat ttggctagtt cttcttcgct gatagtgata gcagcttctt
    12961 tttgataagt taaataattt tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg
    13021 tataccaatt gttattgata ttgttctcat attcgtttgg gccggtcaca ccgtggatca
    13081 tgtatttatt tgctcgtttt gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg
    13141 ctaaaacttc cagtccttca tttttcaaat attccgtatc gccggtgtaa ttcgtataat
    13201 tgtagattgc ataggcaatc gcgccatttc ggtgaatctc ctcgaatgtg atttcccact
    13261 cgttatggca ttcgacacca gtgaaggtga ccataggata aagcgcgcct tttaatcctt
    13321 gttgctgagc gttgtggatt gcttgcggca gttgattatg ccgatatttc agcagattct
    13381 tagttacttc aggtttggct aaagcaagat aaagagggac agcatacgct tctgtatccc
    13441 agtaagtggc tccgccgtat ttttctcctg tgaatccttt ggggccgata ttcaatcgtt
    13501 catcttcgcc gtagtaagta gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg
    13561 cttcttcgtc tccttcgatg accacatcgg caagcttcca tcgttctgcc caagccgctg
    13621 tttgctcttc ttttgcttgg atataattgt ttgtctggtt cgttaattct gagacagctt
    13681 tttctgcttg ctgcgcttct ggaatatctc tactagtaag aacgataatt tctttttcca
    13741 ctgaccatgt ctcaccttga gataattctg cagacaagca accatgtaat gctaaagtat
    13801 cttcgtgata gtcagggatg actgattttc cattaacgaa atggcgcatt gctgcagtaa
    13861 cagtaaattg ttcaatccca aagttgtttg gaatagtttt agtcgtcaaa tagccgattt
    13921 cttctatagt tccacgatca atctcattcc aaaaatgttc atcatagttg ctgtcttcgt
    13981 tttgaacatt tccatccaat tttgatacga gtttgactgt tccgctacct tcaagcattt
    14041 ctatagttaa atagatataa gctgattctt ttttgacaat gctcagaaaa cgttcgaagg
    14101 aaatgcgtac tttattagta gaagtagtga ttgtgaatgt acgagataat acaccatttt
    14161 tcatatctaa ttcccagtaa aagtcttctg gttccaaaga agccaagtcg atcagctgat
    14221 cgttgatcga aatatctatc gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt
    14281 ctggataacc atttttccac cagccgacac gtgtcttatc gggataccag acacccgcca
    14341 aataagtacc ttgatggtgg tcccctgagt aagtttcttc aaagttcccg cgcatcccca
    14401 tataaccgtt tccaatactt gttaaagatt cttgcagtcg aagatttttt ttatctaatt
    14461 gtgtcgtgct gattttccat ggatcaatag caaatagtcg tttgatctgt ttcatttttc
    14521 tcctcctaat gagctgtttt atacgttcat aataaacgca accgattgca caagtcaata
    14581 gacaaaagaa aatatttcat agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta
    14641 tagaagggaa cacacgtatt ataggtttta tagaatggta tagacagaaa aataaaaaat
    14701 aaagcattta caaaaaagaa aacgcacgct ataatacgtt taacgaaacc gattgcgtaa
    14761 aggagctaaa cggaatgaaa aagatattta gttttgagtt ttggcaaaaa tttggaaagg
    14821 ctttaatggt tgtggtagca gttatgccgg cagctgggtt gatgatcagt atagggaaat
    14881 caattccctt gatcaatcct gattggacgc cgcttgtcac aacgggcgga gtaattgaaa
    14941 atattggttg ggcaattatt ggtaatcttc atattttatt tgctttagca ataggaggaa
    15001 gttgggcaaa agaaagagca ggcggagcat ttgcagctgg gatctcattt attttaatca
    15061 atcggatcac tggggctatc tttggtgtca caagtgacat gcttgcgaat gaagatgcat
    15121 ttacccacac tctgttcggt acgaagatca tggttaaagg attttttaca agtgtattgg
    15181 aagcaccagc tttgaatatg ggagtattcg tagggatcat cgctggattt gtcggggcaa
    15241 tggcttataa taagtactat aattatagaa aattgccgga tgctttgtca tttttcaatg
    15301 gaaagcgctt tgttccgttt gttgttatct tatggtcaac aatcgtggca ttgattcttg
    15361 ctgtggtgtg gccaaacgta caggctggaa tcaataattt tggtttatgg attgcagaat
    15421 cacaagaaag cgcaccgatt ctagcaccgt ttttatttgg aacattagaa cgtttactat
    15481 tgccatttgg attgcatcat atgctgacga ttcctatcaa ctatacacag cttggcggat
    15541 cgtatgagat tttatcaggc gcacaggcgg ggacacaagt atttgggcaa gatccgttat
    15601 ggttggcttg ggccactgat ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg
    15661 agtttgtgtt gcataactgg acgccggctc gtttcaaagt aggacaaatg attggttctt
    15721 caggtatctt gatgggaatg gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag
    15781 caagatataa atcaatgtat ttttcagcag cgttggctgt ctttctaaca ggtgtaacag
    15841 aaccattgga gttcatgttt atgtttgctg ctgtgccatt atatgtcatt tattcagtga
    15901 tccaaggggc tgcatttgcg atggcagata tcttgccatt gcgggtccat tcatttggga
    15961 atatcgaatt gctgactcgg acaccgttag ctatcaaagc gggtctaggt ggagatctta
    16021 tcaacttcgt gttgtgtgtg atcgcttttg gtgttgttac gtatttctta gcaaatttct
    16081 tgatcaaaaa attcaacttt gctacaccgg gacgtaacgg aaattacgac aatgacagtg
    16141 aagaaaccgt tgtatctaac agcggaacag gaacagcaga tcagcaagtg gtccagatca
    16201 ttcatttgct gggaggaaaa gaaaatatca aagatgtcga tgcttgtatg actcggttac
    16261 gcgtaagtgt aaatgatcga gaaaaagtag ggactgaaga agcctggaaa cgagcagggg
    16321 cgatgggatt gatcgtcaaa gacaatggag tccaagcagt ttatggacca aaagcagatg
    16381 tcttgaaatc agatatcgaa gatctgctgc aatcaggagc agagattcca gagccagaaa
    16441 tggtttctac aggaaatgga caagcaggta gtaagcaata cttaggtata gaacaagaac
    16501 tagtttccgc agcttctgga gaagtgattc ctttatctga agtaaaagat ccggtattct
    16561 cacaaaaaat gatgggtgac ggctttgctg ttattccaac atcaagagaa gttgttgcgc
    16621 cgatcgctgg aaaagtgaca agtattttcc ctagcaaaca tgcaattggg ctggaaacga
    16681 aagatggtat cgaagttttg atccatatgg gaatcgatac agtccaaatg aatcagccag
    16741 cttttgagat tgctgtcaaa gaaggccaag aagtaggaac aggaacgaaa ttagctgaga
    16801 tggatcttga tgtgatccaa aacgaagaaa aagatccaac gatcatgatc gtatttacaa
    16861 acgataaagt agaagaagta gtgatcaagc aattaggtac agtgacagct ggtaaagtga
    16921 tcggctccat taaaatgtaa ggagaggttg tgaaaaagct gtcctgcatc aagtcataag
    16981 aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgagaag
    17041 cagcagtttg aatactgttt agtggagagg tcgtgaaaaa gctgccctgc accaagtgat
    17101 aaaaacagcc aaacaaaaaa tagctcctgg tccattgagc ttattactga ggagttagca
    17161 ggagaacact atttgctgga gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg
    17221 aaatgaagac aaggagaaaa ccaatgaaat tcatgacact caacacgcat agctggcttg
    17281 agccagaacc agagaaaaaa ttgcaagaat tagcagataa gatcctgtta gaagattatg
    17341 aagtgattgc tttacaagaa atcaaccaat tactggaatc tgaagaagta gaacctgcga
    17401 agctaatgaa gttctgtccg gtaaagaatc aagtacctat ccgtaaagat aattttgcct
    17461 accgtttagt tcagctttta aaggaacacg gaaaagagta tttttggagt tgggaaatga
    17521 gccacatcgg ttatgataaa tatgaagaag gaaatgcact gcttacgaaa aaaacattgg
    17581 aaagtcaggt ccttaccgtc tcccaatttc aaaagaaaga agattatcag acacgcaaga
    17641 tcttgattgg gaaaacaaag atagatgatc aggacatttt tgtctcgagc tgccactttt
    17701 cttggtggac agataaaaaa agtggctttt attttgagtg gaaaaacttg gaaaactact
    17761 ttttggaaac tcgcgtacca ctttttttcc taggtgattt caataatcca gtagactctc
    17821 aaggttatta tactgtaaga gaaagttgcc ttcttcttca agattcgtat gttgtcgcga
    17881 atgaaaaagg aaaagcagct actgtggaaa agaaaataga tggttgggaa caaaatacag
    17941 aaaaattgcg tatagatttt attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac
    18001 ggatttttga tgggatcgat agtccaatca tcagtgatca ctacggggtg gaaatcgaat
    18061 tagatgtgaa cgaataaaaa atataacata taacataaaa aaactgatta aaataaaact
    18121 ctccaaacga ttataccgtt tggagagttt tttgatcgga tgtatttatc ccttttgcct
    18181 aagcacgtaa aagcttgtat ctatgataaa taaataaaca tagcaaggta aaccagccaa
    18241 tcaaagtaag agaaagaatg aaatagaaat agattggcgt ttgataatga agcgtgagct
    18301 cgttgattcc tttttgctgc ttcacgattg gggtcccgat atctgttact tcataatcag
    18361 tcaatgtttt tccattttgc tgtagcacag tccgatcgta gacgataaca gggatatgaa
    18421 cagttttgtc cccagtgttt ttccacttga ttgtcaaaag accgcctgga tgtgttttta
    18481 taaaagttgt ttcgttttct agtatttctt cgttataccg gtcgtattta ttgtcttttg
    18541 tctccttata gattggcaga tagtcaggtg tggttttttg gaaaacgaac aagctggtag
    18601 acatatcttt gctgaagaat gttttccttg cttcttccgg cgtatcgaat agataagtat
    18661 gtcgactgac aaatgtttca ttttcccact tttccagatg ctggtataag ttctggatcg
    18721 tctgtgctga aaaaataaga atgccaaata tacttaaaag acggaaccac ttttgttcag
    18781 accactggtt cagtgaaagg gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa
    18841 atcgaaatgg aaactggatc aaactaataa aaggcacatt tttttcgatc aatgtcgtcc
    18901 atggaatcaa gctagtagaa agaaaaaaga aaacaagact tgtgtacaac acgatttttt
    18961 ggaataaggt gttcttttgt tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata
    19021 taggaaaggc cttagggtaa tggagccaat aactgccatc cagatctatc gccttttgac
    19081 tcatttctct gttaacaaag ggaggaagaa tcttattccc actgtaaaca atagctaatc
    19141 ctgcccaaat atttgctgtc agaacaaaat aaagcaagac cgacttaatc agttgaagca
    19201 gtgcttttcc tttttcagtc tggttaaaaa agtaataaag ataaaaaggg atatacatca
    19261 aagctaacat gatcgaagac agtaaatgga tctgtgtcat aagtcccact gagacagcga
    19321 ctttgataac agggaacttt ttatgaagga cgaaatcagc aatagggatc atacataaag
    19381 gataaaaaca agtcccccag ctagtgaatc cttggttgat agaccagtat tgaatagaat
    19441 acgtactcat gaataaaacg gctactggta cacttaccca atttcgaaca ttgacatatc
    19501 tcagtaaaat cagcatagaa aatcctgaaa taagataaag aatgaaattt gaaagaatct
    19561 gataatgaaa ccagctacct gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg
    19621 ggccgtaaac ggcattcacg atcctgccac tttgctggaa tccataaagg gaaggaaaat
    19681 aatggaaatc cccatattta atttgttgcg cggcatcata gaaccgattg taatggaatg
    19741 gggcatcagc acccaaaatt agtttatgtg tgaataaatg aggcatgaga aatgccaaag
    19801 caatgaaaaa aattgctaaa aatgggccat attttttcaa ttgtcgaact cctttgcaaa
    19861 ctttagataa attcatcata tatcctatgc gagaggtagg caagaaatta gaaaattcat
    19921 gaaaatattt atgaatttta caagaaaaac ataagtgaaa gttctaagga gagaggatgc
    19981 gacataagtt ttgttgcatc tccttgtttt gaaacagaat ttttcttaat actcgaaaca
    20041 aagtagcttt ttcatgacct ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa
    20101 gctcactagc ctagaaaata tgagaagcta ttttctgact agcgctctta ttactcaaag
    20161 cataacgcag ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    20221 ataagcccaa cccaacaaaa aatatgagta gctatttttt tgggctgtac ttattacttg
    20281 atgcaggaca gctttttcac aacctcatcg gctttcattt tctatttttt tcctgaaccc
    20341 atataataaa agaaagaaca aaaaaacttg caagggagga cacatgtgaa gcagagtaag
    20401 caaaaacagt ctcgtcgact tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg
    20461 ttactgatcg ctttatatcc attttatgtg gattctttaa atagttttat tgatcaaaaa
    20521 cgtatcgaac aagtacaaaa acggaccgca gctgaaattg aagtgcagcg aaagaaaatg
    20581 gaggagaaaa accaacgatt aactgaccaa ggattcaatc ctggtactga tcctttcaat
    20641 gaaaagaaca gaaatgaaag tacaacatcc agccaattag aggaatggct gatcggaagt
    20701 atcaatatac ctaagatcca attaaatatt tcactttatg atcggctcaa tggtaggatc
    20761 cttgaaaatg gagcaggcgt cttgcaagga acctctttcc cgcttggtgg aaattcgacg
    20821 cattcagtga tctctgctca cagcggttta cctaatcgac ggttatttac cgaattagat
    20881 cgtttagaaa acggagacac gtttattttg actgttttgg gggagaaatt ggcttatcaa
    20941 gttaaaaata tacaagtggt actgccagat gacacgtctg ttctaacgat ttcagaagga
    21001 gaagacttag ttaccttgct tacatgtacg ccgtatatga tcaacacgca tcgtttgcta
    21061 gtgacaggac atcggattcc ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat
    21121 caggaaagaa ccatgcggca atggtttatt cttgcaggaa cgataatagc tgtagtcata
    21181 ctgttagtgt ttatcggacg actaatctat cagtatagat taagtaaaaa agtgcttgat
    21241 ttttcattta ttattagtga ttcgtcaggg aaaccggtaa atggtagttc attcattttg
    21301 aaacataaga aaaaaacact tactagaaat ggtgtgccgt tttcagtaca aagcgaccat
    21361 catgggaaag tcaagctgga tcaattgcct ggtggaactt atcgaatcgt ttcagctgat
    21421 gatcctaaag ttgctgtttc ttttggtatt agaaaagtaa aacaagagaa gatatacttt
    21481 tttgaggggc gaaaacttgt aaaagagctt caaaaaaatg gtttttattt taaaatcaat
    21541 gaataagtaa ggagagaaaa aatgatccgc acaacaacaa aattgtctgc caaagaactg
    21601 gaatctgttc tatcgatttg gctgcatgct aacttggatt ctcatgaatt cattccatca
    21661 acttattggg agaatcaagt agaatctgtt aggaaagctt taccagatgc agtgatttac
    21721 ttagcagaaa tagatcaaga aattattggt tttgctgggg tgattgatca gtttatcgca
    21781 ggaatctttg tgaaaaggga ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa
    21841 ataaaagaaa ccaataaaac actgactttg acagtctatc aaaaaaatca atcagctatt
    21901 catttctatc tttcacaagg ttttacaatt caaacacagc aaaaagatga agcagtaaat
    21961 gaggaggaac ttgtgatgaa atggaatcaa aacgctgatt tttgatgaac agaataacga
    22021 aataatgctc ccagcctaat tttttgctgg gagcattatt tcattttgaa gtcactaatt
    22081 taactgtgtg aatatgcagt tgttacaatc tttgataaac ggtgttcaat cagctgaccc
    22141 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccattt gtttctctaa
    22201 tactcaggtc aaaacgctga tttcacaacc tcttgataaa cggtgttcaa acggctgctt
    22261 ctcggtaata agcccactag cctagaaaat atgaaaagct attttctaac tagcgttctt
    22321 attactcaaa gcataacgct gatttcacaa cctcttgata aacggtgttc aatcagctga
    22381 ccctcggtat tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc
    22441 taatactcag gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt
    22501 ttataatttt catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt
    22561 tcttcgctaa taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca
    22621 aattcaggaa caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct
    22681 gcgatatgtt cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg
    22741 aaaaagatca ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc
    22801 cctgttggct ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct
    22861 tcttggtctt ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta
    22921 tggtcatttt tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga
    22981 gttccaataa aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat
    23041 taaaacatgt aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag
    23101 atttgtcaag gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg
    23161 gttattaagt aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat
    23221 aggagaattt tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga
    23281 gcaaggaatc attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta
    23341 tgaattaaca gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga
    23401 actaccggtt aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga
    23461 tccggcatta gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca
    23521 tctattagag cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg
    23581 acactacctg attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac
    23641 agatgtatta gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa
    23701 ttcctttcta tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc
    23761 cttttttcaa gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag
    23821 tggcgtttag agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag
    23881 cttgtaagtt aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc
    23941 tttgaagtta ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat
    24001 tgcttttgaa aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc
    24061 tgcagctttt gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat
    24121 gactgtaggt aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa
    24181 tcccttttgt tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc
    24241 actgatgcca acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat
    24301 ttttgtcact ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc
    24361 aagtaggttg acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt
    24421 cagaaagaag gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata
    24481 atcggacact tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga
    24541 ccttattgac gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg
    24601 tgcaatggtt aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat
    24661 ggctgatcaa taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga
    24721 tcggaacgat tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga
    24781 tccaagcttg cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt
    24841 tcccgccaga aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc
    24901 ctgctttagg ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt
    24961 ttggtatggt actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa
    25021 gtgttattcc attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg
    25081 gattcggtag tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga
    25141 aagtaattgg tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat
    25201 tacatttgga acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg
    25261 cagctgtttt agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat
    25321 tgtatattca aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg
    25381 gtgcattgat catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg
    25441 cgaaacgttt agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt
    25501 tcttgacaaa aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg
    25561 ggatctccat ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact
    25621 tgatcagtca tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta
    25681 cagctatttt gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg
    25741 tattggataa tacgccatta gcttataaag atcaggcaat caatgccaca ctgtcaggtt
    25801 accatgccgc gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt
    25861 tattgaagaa aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt
    25921 tttgattgtc agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca
    25981 aaccttttta tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc
    26041 taatttgtcc aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt
    26101 ttccagtgct gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa
    26161 agaaaaagtg tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac
    26221 ggataacgaa acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac
    26281 tacttattgg gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa
    26341 tcacgaatat gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag
    26401 tacggaccaa gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag
    26461 tgaagcaaaa gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat
    26521 gagcaaagca gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc
    26581 attcgcaaaa ttagtgaaag aagcaaaagg cgaataatat tttgaacacc atgtaggagg
    26641 ttgtgaaaaa gctgctctgc atcaagcagt aagaacaatc agataaaaat agcactccat
    26701 atttttcatc tgattgggct tagtgccgta gatgcaagct tttgaacacc gtttaaggaa
    26761 gaggttgtga gcctgccgtt taactctgag tcacaaggag caatttcaaa aaacagcttc
    26821 tcatgtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt
    26881 cgaaaaaagg aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat
    26941 actagatgta aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat
    27001 ctatgcttcc gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa
    27061 agaattcggt gctggaagag ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg
    27121 cagtatcgtg aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact
    27181 acgaaaagat attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt
    27241 gaagcaaaaa taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt
    27301 tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt
    27361 acaaaatttt tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc
    27421 gttcaatcag ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt
    27481 ccattttttt ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat
    27541 ggtttttcac catctttata ataaactggt tctttgatat ggaaatctat tttttccaac
    27601 caatctaaaa tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca
    27661 ttggagatat ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt
    27721 ttgatgatca cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg
    27781 ctagggaaac gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata
    27841 tgtggtgtat ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca
    27901 gcttggatcg tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc
    27961 accctgccaa cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt
    28021 atggtgatta acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg
    28081 tatgatttgc acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa
    28141 tggcgtttca cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc
    28201 acgtgcaatc aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc
    28261 cggtacgatg atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc
    28321 tttgtttgaa taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc
    28381 cagctttaag ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata
    28441 ttcttttagt ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc
    28501 aatcacgatt tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga
    28561 tgtcaaaaca gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt
    28621 ggaattttgc acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag
    28681 tttttgcact tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg
    28741 ggtccaatgg atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac
    28801 gagtgttgaa agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta
    28861 aaatgactgt cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa
    28921 taccttcatc cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac
    28981 ctgcaggagg attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa
    29041 gaatacttgc ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg
    29101 ttgtttgcat gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta
    29161 cagctggatt agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg
    29221 tgttgatatc ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag
    29281 cacgagcaat acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac
    29341 gcaaagctcc tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag
    29401 tcgtgccaat ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat
    29461 tcaaggcttc gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca
    29521 taccgatcgt atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt
    29581 tttctggatc tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag
    29641 tatttgtagg tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa
    29701 cgacttcttg tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta
    29761 cagcgtcttt caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac
    29821 gtttacgact agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt
    29881 tgaaagtcat agctggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat
    29941 aacggtaaac accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt
    30001 gacctggaag tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca
    30061 tgagagattc ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa
    30121 caattcaact tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag
    30181 aataaactat gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa
    30241 agtgaaaaaa atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat
    30301 tttgaaagtt acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga
    30361 gataaccgat gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc
    30421 actttctcgt aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt
    30481 cataaatgga aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat
    30541 accagaatta taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct
    30601 cgtttcttgc tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt
    30661 cacaacctta ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag
    30721 gaagatgacc gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga
    30781 caggatttag ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag
    30841 ggaacgtagc tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac
    30901 acgtttctgc gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta
    30961 caccgatttc ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca
    31021 tacttgttac taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca
    31081 atccgccagt cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat
    31141 ctgatttgcc tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat
    31201 tgttcggata aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg
    31261 tttgtttggc attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt
    31321 gagctttgac gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc
    31381 tataagcgat tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc
    31441 ccgagatctt caattcatca gaagcattct gatacatcgc atcataatct gtattaggga
    31501 taaaattccc agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg
    31561 taccaacgat atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct
    31621 tcaatgcgtt gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg
    31681 aggctggata acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag
    31741 tgtcaagctg tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag
    31801 ataaagaaag agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc
    31861 catttacatc acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta
    31921 agttaggatc gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct
    31981 gtgctttatc ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt
    32041 tgcttgcacc caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa
    32101 tcggaaattt cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag
    32161 aaagggacag aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga
    32221 aagtacgtcc tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac
    32281 tcatctttgt tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg
    32341 aatctgttaa gatattacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca
    32401 gttcaggatt atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt
    32461 ccatgatctg gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga
    32521 tatctgggtc attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata
    32581 actgatttgg ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg
    32641 cccgttttcg tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt
    32701 caataatgcc tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt
    32761 ttcggtaggc atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca
    32821 tatcaccgga atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag
    32881 aaccactagg acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat
    32941 ccaatgcttt ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa
    33001 gcataatcac ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa
    33061 tgatcgtagt atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa
    33121 tttttcaaaa aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca
    33181 attacagttg ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt
    33241 gaatggagag gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga
    33301 tatcatgatg ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt
    33361 tgacacacct gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt
    33421 caaattagga gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg
    33481 tgtggaagca ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca
    33541 aacaaaatta gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc
    33601 tcaaaaagaa tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg
    33661 ccaacagttg atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga
    33721 tgtacatatc aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac
    33781 agtacgggga ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa
    33841 ttatggatct attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt
    33901 ctcggtttta ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa
    33961 ccaacgttat acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata
    34021 tcgatttttg ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt
    34081 ctggtagcaa cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta
    34141 cagcaactgt ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg
    34201 aggaatgact ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa
    34261 ggatatgttc aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta
    34321 tatcggatta tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg
    34381 aacaaactgg aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa
    34441 atcagagaag taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat
    34501 atcgaacttg ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg
    34561 aatatcatgg ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta
    34621 tttgagacag ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa
    34681 gagaccagag atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct
    34741 gggaatgggc taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt
    34801 gattatagca gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag
    34861 gaaaaagaat aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt
    34921 tttgtgatta gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt
    34981 gaactacgag aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg
    35041 aaagtcatgc gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag
    35101 ttttcttctt gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact
    35161 ttacgagtaa atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt
    35221 tcttttaata gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca
    35281 atcatattga tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca
    35341 gcttcgcgac acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa
    35401 aagttctcct ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt
    35461 ttaggtagaa ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt
    35521 ggggcctgga aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa
    35581 gttaattcga cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa
    35641 aactcggtga ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt
    35701 aatgaaagat tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt
    35761 tctacgacat tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt
    35821 ctatgggcgc gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta
    35881 gaaatatttc cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg
    35941 gctaaatcaa gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca
    36001 ataaagaata ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct
    36061 ttcttgcagc gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa
    36121 agagaggagt tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg
    36181 cggactaatt ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac
    36241 gatggttttt ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa
    36301 tatgccggct gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt
    36361 aggtaatggg ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc
    36421 tagtgaaaag cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt
    36481 tctattttgt gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga
    36541 cagtacgatt ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg
    36601 taatttaggg tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt
    36661 gttcttattg gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa
    36721 agcttgcggc ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt
    36781 cccagtagct gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac
    36841 aaattggatt atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa
    36901 aaaggatcaa atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat
    36961 tatatgttgt ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta
    37021 atgcacatcc agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag
    37081 gaagatatgt atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa
    37141 tctacactat atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat
    37201 gaaccttttg aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat
    37261 ttagaagaaa ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag
    37321 gagagcacaa atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga
    37381 agacgatcgt tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac
    37441 attagatcag atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat
    37501 cagttacttt tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca
    37561 aattcgcctg atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact
    37621 agcactgggg tttatattgg ttattggggg actattggtt gctttactag cgctcatttt
    37681 tagtatctat gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg
    37741 aatcagtgtg tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt
    37801 cgccacagga agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt
    37861 ttatgtcatg atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct
    37921 gcattcagcg ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac
    37981 cggtattggt ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc
    38041 tcaaaagagt ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa
    38101 aaaaatcaag gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat
    38161 acgcggattg gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa
    38221 ggagaaaaat ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag
    38281 tttagaagac acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga
    38341 attgacaatc gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt
    38401 gaatactaag acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac
    38461 tgcgaaagat gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt
    38521 cacaggtgaa aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt
    38581 catcctaaac ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca
    38641 aacgtctggt gaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga
    38701 acaaggacag aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga
    38761 tcaagaggac aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag
    38821 caaagatcct tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat
    38881 aagaaagaaa atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt
    38941 caaagagaaa agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc
    39001 caaaccttct atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa
    39061 ggtcgccaat tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa
    39121 gagctggatt attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag
    39181 aaggaacgat ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat
    39241 ctagaggtct tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg
    39301 gattatttac cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac
    39361 aaagagttag aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac
    39421 attagatatt tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat
    39481 tttatatcaa tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt
    39541 ttttcttcta tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc
    39601 tttggttgat cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa
    39661 aagtaatttt gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta
    39721 cgataccttt ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg
    39781 ttttaaaagt caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta
    39841 aagtcgttga tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa
    39901 tccaaagtga gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg
    39961 agaatgattt gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc
    40021 tgcaagagcg tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag
    40081 cctatgcaac agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt
    40141 ctgctcgaca aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa
    40201 tcgaagatgg acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt
    40261 tacttgtctc aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga
    40321 aagaccgtaa gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg
    40381 tcttgaactc tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag
    40441 ttgattcgaa ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa
    40501 ggggaaaaag tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca
    40561 ggggtgaaaa tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg
    40621 acagatggta caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga
    40681 tatatcctgc ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa
    40741 ttgactcgac cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt
    40801 gtcacttcgt atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta
    40861 attaaagggt agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat
    40921 acggaaaaaa caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa
    40981 aaggcgaatt tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata
    41041 ttttatccac attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga
    41101 ccacattaaa aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc
    41161 aagatttcaa tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt
    41221 tacaaggcgt cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat
    41281 ccatctctca tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg
    41341 tcgccgccgc acgtgcattg atcacacaac caacgattct attaggggat gagcctacag
    41401 gtgcgctgga ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa
    41461 aagatcatgt atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac
    41521 gtattttatt catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc
    41581 gtgattcctt ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga
    41641 ttgactatgc tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg
    41701 gtgttattct caggattagt tatggcatct gctatttttt acatgtttga atcaatggca
    41761 agcaacgaag cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga
    41821 ttcggttctg ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt
    41881 ttgatgacga tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga
    41941 aaaatcgcac agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg
    42001 ggttcagctg tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta
    42061 gatctcaata tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg
    42121 ttttttgctg ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa
    42181 ccgattttag cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc
    42241 ttgtttttcc ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg
    42301 aaacacgtgg ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta
    42361 ggaacttatt tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca
    42421 gaacagattt cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt
    42481 cgtgaatata cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct
    42541 ttgacggtag gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga
    42601 tatgatctag tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca
    42661 ccaacacttt ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca
    42721 agtgaatttg cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag
    42781 atcacgaaag aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt
    42841 cgaagctatg aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa
    42901 tttgatcaac tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa
    42961 gcaaacctga aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg
    43021 caaaaagtag agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc
    43081 tctggttttg aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc
    43141 tgtttgatgt tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt
    43201 gaaaagatag gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg
    43261 ttattcttag caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt
    43321 ggtctattca tgcaaaatcc atacaaagat atttgggttc cgtttacgat cttcttcgta
    43381 ttgtatttca tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag
    43441 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct
    43501 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt
    43561 cggaaaaaga ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag
    43621 aatatagtaa attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg
    43681 tatactggaa tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa
    43741 caaaattttg gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg
    43801 tctgtcagtg atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa
    43861 cgagagttga tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc
    43921 ggtgcttcaa tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat
    43981 gaaacgttcg aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct
    44041 agctttgaag ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc
    44101 attttctatc agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa
    44161 gtacggtata cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac
    44221 cgcaaaacag tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca
    44281 cagtcaatcg acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa
    44341 gatacaacgc ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt
    44401 cctaaaaaat taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc
    44461 atcgatcatc catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca
    44521 agcccagatg aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt
    44581 acagcaaatt ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc
    44641 ggtatcacat ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat
    44701 gtcactctgg aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag
    44761 aaatagaata aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta
    44821 tttctttaaa ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg
    44881 aacagttgca gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt
    44941 gttccacaaa tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt
    45001 tatcgttgaa aattctaatc ctgtgattgg cacatcgaat cgcttagaca tattgaggat
    45061 ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag
    45121 gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg
    45181 tccttccaaa tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt
    45241 gtagaaatca ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact
    45301 ttccttgtaa gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact
    45361 atcttgttca agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa
    45421 aaggttatga tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc
    45481 ttgagccatc atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc
    45541 tgcaatcttt ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc
    45601 taatccatct aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc
    45661 taagtttact cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt
    45721 atctcctggt tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg
    45781 agtaacaccg gaagaaggtc cccagaagac gagatcgcca ggttgggcct gacttactgg
    45841 aatctgcgta cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc
    45901 tttcatatat acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc
    45961 ccatacatat ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt
    46021 cgttgtattt gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc
    46081 agaagatgga ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt
    46141 cgttccgcca ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt
    46201 gttattcgat ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt
    46261 attattgttt gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt
    46321 tggcgtttcc ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt
    46381 tgaagtatcc ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt
    46441 aaaggtcttt gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg
    46501 ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac
    46561 cgttccactt tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt
    46621 cagttggatc ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt
    46681 ttcattttct gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga
    46741 gttagttgtt ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct
    46801 ggatgtagtc gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac
    46861 ggtattggtc gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt
    46921 ttttttattt ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag
    46981 cattcattag acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa
    47041 tctgtctgtg tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt
    47101 tctttgacgt tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata
    47161 aaaatggtta tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa
    47221 aaataatgat cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc
    47281 tttttcatta actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg
    47341 atcattgcta cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa
    47401 atgaatgcag gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca
    47461 aatcccatta cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc
    47521 aatgggatcg caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca
    47581 aaagcagaac cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa
    47641 gctgcaccag ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca
    47701 taagcagaga tattactgat catgacaggg tttacagccc attggatacc aaatattacc
    47761 aaaatattcc aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc
    47821 caagtaattg cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt
    47881 aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt
    47941 tcgcgaatat attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc
    48001 gccggcatga cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta
    48061 gctgctttgc cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca
    48121 atgacagccg caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag
    48181 attggcagaa aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt
    48241 gtttcactaa gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa
    48301 ccggcaaaca gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg
    48361 atcggattta gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa
    48421 gcaggagcca atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac
    48481 ttaatggaag ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat
    48541 ttgatacgaa gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac
    48601 tgttcaatca gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt
    48661 tccattagaa gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg
    48721 tacgaataaa tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc
    48781 taatcggtgt atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct
    48841 ttatttcttc cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa
    48901 cagataaaaa acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg
    48961 ggacaggcat gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta
    49021 ctcgtgaaat atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt
    49081 gttaatttta gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac
    49141 cataagactg cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt
    49201 tactaaggtc tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg
    49261 ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat
    49321 agaaagcgcc ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt
    49381 tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg
    49441 tttctgtttc atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt
    49501 cacgagctgg tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag
    49561 ctaggatcgt cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa
    49621 tcagttgctt gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga
    49681 tcaagatact attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc
    49741 cttggatttc agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact
    49801 gttgtccttc gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt
    49861 tggctgccac tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat
    49921 gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac
    49981 ttccaattac tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg
    50041 ggaaaattat tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc
    50101 acaccttgaa agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa
    50161 tcttttttct tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga
    50221 tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc
    50281 gcacatatct atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc
    50341 ttcctctaat ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt
    50401 tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt
    50461 aggtaagagg atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc
    50521 ctcaatccga gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg
    50581 atgtcctaga ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg
    50641 tacggcatca ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg
    50701 ttttttcaaa ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc
    50761 atccacacct cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc
    50821 agcattacat aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat
    50881 cagtgtactg aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc
    50941 cttggtaatc atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc
    51001 taaaactttc tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga
    51061 gacagttgtg attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt
    51121 caccatgttc atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag
    51181 taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt
    51241 ttcgttgagc gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat
    51301 tctttgtcac gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag
    51361 gcatctaatg cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt
    51421 tcgattccac gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg
    51481 tattttgtga tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca
    51541 gtgaattctg gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa
    51601 gggaaagtgg agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt
    51661 cttactaaca aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg
    51721 ttatttacga tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa
    51781 atcatatttt cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat
    51841 gtaagtaaat cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct
    51901 agagaaattc agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag
    51961 cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata
    52021 caaacgatac aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat
    52081 tgaaaaaggc gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct
    52141 taatatttta ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga
    52201 tattgcgcaa ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt
    52261 tttccaattc tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac
    52321 ggaagtttct cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca
    52381 tcgattgaat aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc
    52441 tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga
    52501 ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa
    52561 taccgttttg atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca
    52621 cattaatgat gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga
    52681 gattgtatgg tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga
    52741 aattcggcaa tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact
    52801 tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt
    52861 tggtacagca ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga
    52921 tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta
    52981 ttccaatact ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca
    53041 gaacaagcta caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga
    53101 tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga
    53161 attgaaagaa aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa
    53221 tttggagaga ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc
    53281 tgaatccgtt tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa
    53341 gaatttgaat acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc
    53401 agaaaagtat ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa
    53461 cgaagaatta gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc
    53521 aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca
    53581 gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact
    53641 tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga
    53701 attgaatcga aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga
    53761 acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat
    53821 tgccaatgtt tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat
    53881 gacacgtatg gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac
    53941 gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat
    54001 tgttttagga acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa
    54061 cgagcggtat gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc
    54121 agctgccgca ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt
    54181 aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt
    54241 tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg
    54301 aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc
    54361 cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca
    54421 atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag
    54481 tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat
    54541 tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat
    54601 cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa
    54661 cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga
    54721 cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga
    54781 agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta
    54841 tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat
    54901 gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa
    54961 tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc
    55021 ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct
    55081 aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt
    55141 ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg
    55201 aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc
    55261 tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg
    55321 attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat
    55381 cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcgttt
    55441 tgagctaata acgagggtca gctgctaatt gaacaccatg aatcagaggt tgtaaaaaag
    55501 ccgtcctgca tcgagcaaaa agaatactca aataaaatta gctcctcatg ttttacattt
    55561 gagaagactg attgccgtag ataaaagctt ttgaacacca tttatagaag aaacaagaat
    55621 attttgctat tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg
    55681 ggcaacagac ctcttagaat gtataaagat aaattttaca aacgctttcg gttcagttat
    55741 aataataaat gataacttgt atatataagt tgtggtaggt aataaatcag ttgaatggag
    55801 gtaaatatcc atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa
    55861 agacagtaat ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct
    55921 gaatgatcta ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga
    55981 caacggttat gatatctctg attacacagc catcgatcct atctttggta cgatggagga
    56041 attcgaacag ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt
    56101 gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca
    56161 ttatcaggac ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg
    56221 aagtgcttgg tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac
    56281 acaagctgac ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg
    56341 tttttggatg gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa
    56401 agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc
    56461 aatcacccat acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat
    56521 catcacagtc ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc
    56581 tagtagagaa gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa
    56641 tgggaataaa tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac
    56701 atggggagaa aagatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga
    56761 tcagccacgt gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac aaggagcaga
    56821 gatgctagca accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga
    56881 aatcggcatg atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt
    56941 gaatgcttat aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat
    57001 caaagcaaaa tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc
    57061 aggatttact acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt
    57121 agaaaaagaa ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct
    57181 gagaaaaaca tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc
    57241 ggaagtttat ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt
    57301 ctatgataaa gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat
    57361 cgataattat tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat
    57421 tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta
    57481 agatcgacgt tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata
    57541 ctctccttta tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt
    57601 aacgagtaat ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat
    57661 tagatcaccc atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc
    57721 acccattact cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat
    57781 gacaaatttg acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa
    57841 ggcgataaac ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac
    57901 actagcaaat tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa
    57961 tgaagcaatg atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa
    58021 atttttaaaa tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt
    58081 ttgaggatct ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat
    58141 agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc
    58201 aataaatcca gctatatcag acgtcacgtc gtcaatagaa gcaaaaaatt gattaaatga
    58261 atcggcaaaa ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa
    58321 atctggcaaa ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat
    58381 gcttccaagc cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct
    58441 cgaaaggtgc ttgctcaaac atttgatgac tggttccaaa agataataca agatcagtgc
    58501 aaacaataat gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga
    58561 gatctgatgg aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta
    58621 agaggttttt cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac
    58681 tctttttcct cctttatcta taacttcatt atagacaaaa gcgggtataa aaggaaaatg
    58741 atagaggttg attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa
    58801 taagaaaacg cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg
    58861 taactattac ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag
    58921 gagtgtcata aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt
    58981 taactgcttg cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga
    59041 cgacctttta tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag
    59101 agttattgat tccagcaggg actgattccc acgattatga accttctgct aaagacatgg
    59161 caaagatcca agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc
    59221 ccgcaatcca aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta
    59281 tgcttttact tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag
    59341 gccatacaca tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg
    59401 tagcaaacat acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa
    59461 aaaatgcagc agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct
    59521 ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt
    59581 tagaatatgg attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg
    59641 cggcgagaat tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt
    59701 ttgaagaaaa cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt
    59761 tagaagtatt gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact
    59821 atctttctat catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc
    59881 cgctagaaga tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag
    59941 acgaggatgt acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata
    60001 cgtatgtaga agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg
    60061 ataagacagc acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt
    60121 caatcaagat cagtgagaaa gagatggcat ttacgtttaa ggatggaaca acgaaacgtt
    60181 caaattatcg ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa
    60241 ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg
    60301 atcacacgat tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc
    60361 aagaagtatt gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg
    60421 gacgtgaaat tgcacaggaa atgcttttgc actgataggc aaaaaagaac cgaagggttt
    60481 ggatcggctg gtcatttagt cggtccaaac tattcggttc tttttatgaa atcaatttct
    60541 ttaatccacg gatagaggct gggatggtct cgccgtttgc caacgtgact tctctggttt
    60601 ttttattaat cgaccgcaca ttttgtgtat tgataacata ggatttatga caacggataa
    60661 aattccgatg cattttctcg atttctttga tcttctcata gaattgtaat tgggcatgag
    60721 tcaaatggac ttccagttta tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt
    60781 tgatcggtaa aaatttgatc tcatcattaa actctatttt gaagatatct ttttggattt
    60841 gggacatttg aaaatcttcg aaaattttgc tcaggatatg ctcgacattt tcttttaaat
    60901 cataaagatc ttctttgacg atataatcag tcggttcgat gttgcttttt aagattttca
    60961 tcgacatttc cgtatgggaa gtcacataga ttatttttcc taaaggatct aattcacgga
    61021 tcaatttacc gatcgctaca ccatccattt cactgtcttc tagttcgata tctaaaaagt
    61081 aaataccaaa agaagaagtt ctgatcttgg ctgcattgag aagttcttcc ggatcagagg
    61141 tagaaatatg gacataactg tctaagtttt caatcatgat tctatttttg atcaccgttg
    61201 ataaaacttc taattgtttt ggatcgtctt cacaaatata aatgggaatc atatcattac
    61261 tcctttattt tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt
    61321 caaggaaaag ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt
    61381 cgttttttgg atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg
    61441 tatttgttgt tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca
    61501 attcgatttt tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac
    61561 caagcatacg tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat
    61621 gaagctggac gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac
    61681 ggatctcagg aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca
    61741 tctggcgtgt aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt
    61801 ctgaaatata gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca
    61861 agtcttgata gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct
    61921 ctaataactg atcagctaat ttcttattag agattttcca ataataaacc aagatgatca
    61981 ggccaacaca cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt
    62041 caccagaaaa acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga
    62101 tcgtaccgat catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac
    62161 aaaaagctaa taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat
    62221 ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc gtcgatgctt gttaagtgat
    62281 ctcctaaaag cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag
    62341 tcgaaaagaa gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc
    62401 ctaaaatcga atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca
    62461 gatatttgta tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt
    62521 gcaacgctat ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata
    62581 gcacaattag cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca
    62641 aagcagcagc cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt
    62701 ttcatgaaag attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat
    62761 ttctaaaaca atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataaagt
    62821 aatgagagaa aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc
    62881 gggttcaatg ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca
    62941 gctgattaac aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac
    63001 caagggaacg ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga
    63061 atgcaatcca taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc
    63121 cgcccatgag cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt
    63181 caataaaact tctaaggcaa attttacttg gacatacacc atatcttctt ccgatttgtc
    63241 tccgctgaat aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc
    63301 tccaccacaa attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt
    63361 tttgacatca aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca
    63421 aaaaaagtga cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag
    63481 tgatttatgt taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa
    63541 tgaacaattg tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc
    63601 aataaaagga aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg
    63661 cgcagtagaa gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc
    63721 aagtacaata gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct
    63781 cccgtttagc tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa
    63841 tattgccttg tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg
    63901 acaagacttt tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag
    63961 agaagtagct tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta
    64021 aaatgaagaa cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga
    64081 acgatcggtg cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa
    64141 cctttttatg agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa
    64201 aagtatactt ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat
    64261 gctttcagcc atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt
    64321 catttgaatg cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt
    64381 ttagaaacga tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg
    64441 gtttatattc gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa
    64501 tatgaacagc tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga
    64561 tacgaagagt ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa
    64621 tatgatttcg tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg
    64681 gcaaagaaaa tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg
    64741 aaagcaaagg gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca
    64801 gatatttctc tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg
    64861 aaccgattcg tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa
    64921 aataaaatgg aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg
    64981 acaatagctc ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa
    65041 tctgcttttt ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt
    65101 ccagttgtaa ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg
    65161 atcgatgcag taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc
    65221 aaactaacaa agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt
    65281 ttttcttttt ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag
    65341 aaaagggtag acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg
    65401 atcgatagaa tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta
    65461 taactgtcca tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga
    65521 aaggaagtct gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc
    65581 ataaacgatt gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg cttcccattt
    65641 tcatttttag aaaggtctgt tagtataaag aaaaaataga caacgaaact ttaaataacc
    65701 aacttgaaat aaacaaattc aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa
    65761 aaagaaattc tccgattaca aaaagaattg aaacgctggg agaacaagta taaaaagctt
    65821 caacaagtct ttcggctaca agaagaaatc attgctacct atgaagcggc ttatggcgaa
    65881 gcacccggac ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc
    65941 tttaaaatga tggagaaacg ttcctataat cgcttcaatc aagaacaaaa agcagctatc
    66001 acatatgata tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa
    66061 acaatctgtg caaaagctgt ttatctgatg acgcaaaaac aagtagacga agaacgcata
    66121 ctaatgatca cttttacaag gaacgctgca aatgaactaa aaaaacgagt agataatttc
    66181 agccagagaa aaaccgctgt ccatatcgga acattccata gtatcttttt taggatatac
    66241 aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg agacttttca
    66301 aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa atataatctt
    66361 tatatatttg atcaatatgg agaaaaaacg attgcttctc ggttagacta ttggcagaat
    66421 atgaaccttt cagtagaaga aatggttcag ttagttaaag aaaaatatga ttccattgat
    66481 aagaatgcac gacagccgat atcagaacga ctgtatggat tgctcacgga actccaagaa
    66541 caaaaaagaa gccagcaatt gctagagttc aatgatgtac tccaaaatct gaaaagcgct
    66601 ttggagaatg aggagatcca gcattatatc ggccaaaaat atgattatct attcattgat
    66661 gaatttcaag atactaatcc acttcaatgg gaaatcgtga aacggatgac aaaggaaaac
    66721 aggatcaagt tgattgttgt tggagatgat gatcagagta tctatggatt cagaggatca
    66781 gagccagcat acatcaagaa ctttgaaaaa gaatatccta ccaaaactct tttcttatta
    66841 actaattatc gttctcgggc agctattgtc caaggagcaa atagattgat cagctataat
    66901 aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa
    66961 gcctatttgt tttctgatac taaagcagaa agtcagtggc taatcaaaca aattcagaca
    67021 tttattcaaa aaaatgggaa atataaggaa tcaatcatat tataccgatc agcttcacaa
    67081 acgcagcagc ttgtccaatc tctgctgaaa acgaatattc cttttgtact ggaagcagat
    67141 agtccatatg aaggtatctt tggtatcaag tctttccaac atttctatca aaaaattgtt
    67201 cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg cctaccagca attgctccgg
    67261 cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa
    67321 tatcaaaata tagccgttgc agattatatt ttgtcgataa gccccaattt aaaatcgaaa
    67381 tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc
    67441 tattcgcttg ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat cgaccagagt
    67501 gatcaagaat ggatacgaga agaagtaaag cagcatgcta ctttcagaag actccaagcc
    67561 ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa cccgcttgat tgcttacaga
    67621 caaggaaaac ttgatgcact atgtatccag agcattcaca aaagtaaagg tcttagttac
    67681 cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa
    67741 aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag tgacgacgat agaagaagaa
    67801 cgaagactat tttatgttgc gatgactaga gcaagaaatc gtctgtatct atgtgtacct
    67861 caaatgaaaa attcaagaaa attgaaagta tcgagattta tcaaagaaac cgggagtcgt
    67921 gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga
    67981 agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa atttcagctt attttcgaag
    68041 aagctatttc tggaacacag tttattcgga aaagggaggt gtgacaa
//
LOCUS       DAJSNV010000004        63491 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSNV010000004 DAJSNV010000000
VERSION     DAJSNV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.004"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(451..1761)
                     /locus_tag="ON516_000198"
     CDS             complement(451..1761)
                     /locus_tag="ON516_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3343716.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ON516_000199"
     CDS             complement(1773..3191)
                     /locus_tag="ON516_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3343717.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ON516_000200"
     CDS             complement(3431..4999)
                     /locus_tag="ON516_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3343718.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ON516_000201"
     CDS             complement(5003..5413)
                     /locus_tag="ON516_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3343719.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ON516_000202"
     CDS             complement(5410..6333)
                     /locus_tag="ON516_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3343720.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ON516_000203"
     CDS             complement(6330..7208)
                     /locus_tag="ON516_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3343721.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ON516_000204"
     CDS             complement(7195..8532)
                     /locus_tag="ON516_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3343722.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ON516_000205"
     CDS             complement(8535..9638)
                     /locus_tag="ON516_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3343723.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ON516_000206"
     CDS             complement(9712..11220)
                     /locus_tag="ON516_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3343724.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ON516_000207"
     CDS             complement(11186..11878)
                     /locus_tag="ON516_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3343725.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ON516_000208"
     CDS             complement(11900..12874)
                     /locus_tag="ON516_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3343726.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ON516_000209"
     CDS             13174..14046
                     /locus_tag="ON516_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3343727.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ON516_000210"
     CDS             complement(14504..16702)
                     /locus_tag="ON516_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3343728.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ON516_000211"
     CDS             16934..17926
                     /locus_tag="ON516_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3343729.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ON516_000212"
     CDS             17986..19407
                     /locus_tag="ON516_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3343730.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ON516_000213"
     CDS             19400..20899
                     /locus_tag="ON516_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3343731.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ON516_000214"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ON516_000214"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3343732.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ON516_000215"
     CDS             22491..23174
                     /locus_tag="ON516_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3343733.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ON516_000216"
     CDS             23176..24012
                     /locus_tag="ON516_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3343734.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ON516_000217"
     CDS             24023..25777
                     /locus_tag="ON516_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343735.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ON516_000218"
     CDS             complement(26053..26394)
                     /locus_tag="ON516_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3343736.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ON516_000219"
     CDS             complement(26462..28633)
                     /locus_tag="ON516_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3343737.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="ON516_000220"
     CDS             28802..29701
                     /locus_tag="ON516_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3343738.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ON516_000221"
     CDS             complement(29772..30629)
                     /locus_tag="ON516_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3343739.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ON516_000222"
     CDS             complement(30614..33316)
                     /locus_tag="ON516_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3343740.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ON516_000223"
     CDS             complement(33329..34618)
                     /locus_tag="ON516_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3343741.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ON516_000224"
     CDS             34768..35814
                     /locus_tag="ON516_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3343742.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ON516_000225"
     CDS             35816..37969
                     /locus_tag="ON516_000225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3343743.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="ON516_000226"
     CDS             complement(38442..39893)
                     /locus_tag="ON516_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3343744.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="ON516_000227"
     CDS             complement(39890..41614)
                     /locus_tag="ON516_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3343745.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="ON516_000228"
     CDS             complement(41607..42221)
                     /locus_tag="ON516_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3343746.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="ON516_000229"
     CDS             complement(42566..44023)
                     /locus_tag="ON516_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3343747.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="ON516_000230"
     CDS             complement(44064..44984)
                     /locus_tag="ON516_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3343748.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="ON516_000231"
     CDS             complement(44996..45943)
                     /locus_tag="ON516_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3343749.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="ON516_000232"
     CDS             complement(46422..47141)
                     /locus_tag="ON516_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3343750.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="ON516_000233"
     CDS             complement(47190..48836)
                     /locus_tag="ON516_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3343751.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="ON516_000234"
     CDS             49015..49605
                     /locus_tag="ON516_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3343752.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="ON516_000235"
     CDS             49697..51202
                     /locus_tag="ON516_000235"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3343753.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ON516_000236"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ON516_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3343754.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="ON516_000237"
     CDS             complement(52638..53156)
                     /locus_tag="ON516_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343755.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="ON516_000238"
     CDS             complement(53172..53498)
                     /locus_tag="ON516_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3343756.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="ON516_000239"
     CDS             complement(53511..55490)
                     /locus_tag="ON516_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3343757.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="ON516_000240"
     CDS             complement(55511..55825)
                     /locus_tag="ON516_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3343758.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="ON516_000241"
     CDS             55993..56286
                     /locus_tag="ON516_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3343759.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="ON516_000242"
     CDS             complement(56364..57476)
                     /locus_tag="ON516_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3343760.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57863..58042
                     /locus_tag="ON516_000243"
     CDS             57863..58042
                     /locus_tag="ON516_000243"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343761.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="ON516_000244"
     CDS             58236..58406
                     /locus_tag="ON516_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3343762.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..62231)
                     /locus_tag="ON516_000245"
     CDS             complement(58830..62231)
                     /locus_tag="ON516_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3343763.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(62903..63171)
                     /locus_tag="ON516_000246"
                     /pseudo
     CDS             complement(62903..63171)
                     /locus_tag="ON516_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT        18875 a        13061 c        11045 g        20510 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcggcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt
    59641 ggcgcttgag tttctaccag ctgatatgct cctggtgcta aaccgtcaat tgccagtttt
    59701 ccatcttctc ctgtagttag accggtttgt agcgtttctc cttctcggtt ctgtaactca
    59761 aaaacagctc cttgcaggat ttccccgctt tggtcatcga tctttgtcaa cacgacaccg
    59821 cctggtgtaa gtcggttttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc
    59881 tcaaactcga ttggtgtcgc atctaattca taccctattg gcgcttgagt ttctaccagc
    59941 tgatatgctc ctggtgctaa accgtcaatt gccagttttc catcttctcc tgtagttaaa
    60001 ccggtttgta gcgtttctcc ttctcggttc tgtaactcaa aaacagctcc ttgaaggact
    60061 tcaccactgc cttcagcatg tttaattagt tcaacaccac ccgtatcaag gatgttttca
    60121 atggttaacg tcacttcttc tatggagtat tcatttataa aaacatcatg taccgtcgca
    60181 tctaaaagat acccttccgg tgcttctgtt tctcggactt gatagctacc tacaggaagc
    60241 ggttcactta aaaattctcc gttttctgcc gttatacctt cttgtatcac ttgtgaatcg
    60301 cttgataaaa tttcaaattt tgctcctgcc agcctttggc cattctcatc agttttaatt
    60361 actttaatac gcccatcgat tttccttgtt cttacagtac tcgtattcaa tcgattgtca
    60421 ctattcacaa gcgttgcaaa gtttaaaatt tgatcgccga ccgcatacgc ctctacattg
    60481 accttaaaag agagggtaaa acttgtcgga ccaccaatcg tattattcgc actgaaacga
    60541 actattccat tattgacttc ggcatcaatg aaagagggaa tatctgatag aaatgacaca
    60601 aattccatgc cttcttccaa aacatcttca ataacaagcg tatttcttgt tccagaaaac
    60661 atcgagacat gcagagtata cgttagttca ttttcattga gattgataac atttttgtcc
    60721 acactttttc ttaaagagat tgccggaaga ttactttcag ggattctcaa gcggtagtct
    60781 cggctcatta cctgattatt gtaggtcata ctcgctcgat tccgagaaaa cccatcctct
    60841 tgtcttcctt cattgaacaa gtcgacatcg gtcacttcaa gagcatattc aataaaatac
    60901 gattgaccct ctgctagatt accaaagtcc acaacaagtg tattcgtttc cttattaaaa
    60961 gatactttgt ccacaaattc ctcggaaaca taatgagttc taacgtttgc ttcacctgga
    61021 ttcgttactt ctgcgatccg aaccccacct gaaataggtt ctctatttcc agtggaactc
    61081 catgtagtcg gattcggtgc caaagttgtt ccttgcggta gtacatcggt aaccactaca
    61141 ccgtcttttc gtgatccttc ataatttacc tgcaaaataa atttattact gttggtcacg
    61201 tcttgcccgt caatcagcag attcccaaga tttccttggc tgtcgatttc tctagagcca
    61261 tagtataatt tcgaaaaaat gggtcttgat gaaggaatag gcgtaattga aaattccgtg
    61321 tttgctgctc gctcagcata agtaagactc acttgaaagt cattatttgg ctcagtaccg
    61381 cctgattgaa agaaagcaac ttcaaacgtg atactgaatg tatacgactg agcggcttca
    61441 tttaatcctt cataatcaaa ggtaaatgga aaaacaacgt ctgtgccttc ttggtaaaat
    61501 gccagtggct ttaaaggtga atcatcagcg acctccactc taatgaactc aattaagcca
    61561 atcgatgctc taggaactcg tacgaccatc tccccatatt cttcaagaat atcgccagcg
    61621 cctccttgaa tgtatccctt taacgtttgt gtatgcctgc tcggcaaagt ggggcgctca
    61681 gcagagatgc cgatgtcata attcaagagc tcctcttgtc tttcaatctg agattcctgc
    61741 gttatagatg gctcagatga tctggcttca ttaaaagatt cacttaagct ttcttctggt
    61801 atattaaaag attcacttgt gctttcttct ggtacattaa aagactcact tgtgctttct
    61861 tctggtacac tgatttctat tgagaccgta tttgattgaa tatcaccgct tcttgcggtc
    61921 aattcgtatt tacctgaatg ctgggaagaa taagtaatca gacgttcgta atcattgagt
    61981 tgcttcactt ccccttcaat caaatcctca ctcttatcaa tgaccaatgc atttagcagg
    62041 tctggtgttc ctctgactcg caactggaac tgctcaccca aagcagcctc ctcttgatca
    62101 acttcgatcg tcagcgaacg ctctggctgt tctgctaaag actcagctag aaccgttatg
    62161 ttcccaaatt gattgatcac aataagcata actagaaaca tacttaacag tttatgaaat
    62221 ttgttcctca ctattactcc cccttataat tttgactaaa caacaaggta cccgataatt
    62281 ttctaaaaag attccccaac tcaaaatggc tgcgttacct ccctcgtatt ttcggttcaa
    62341 ctcaagcgtt gcaatgtaag ctgtaaacaa agctaatgat tgccgattgt cttctgttga
    62401 ttgattcgat agctttgtat gacttttagt tgtccgtgtg gcttaaccat aattatgatt
    62461 tcacgattcc tcctgccagt gccttgcaca agctggagcg taaccctaat agttattttg
    62521 ttaatgtact tatatacttt tatacttttc cattagaaac aatcactaaa tcgttgatac
    62581 caaggtcaga ctctagaatt tcatcagtga gtccctgtat tggttaacct tcatcaatcc
    62641 caaaaaaata tatcaatgct tagcatcaaa atgaatatat agtttagaat acttgggatt
    62701 catcgataac tgatcgctta tttggccata ttatttggca cttggttcaa ctaaacgcat
    62761 ttaggatgtt ccttcattct cgtcatggat gttctcaaac cgtcattact aataaactgt
    62821 ccaccgaatc gatggttcat ttcttgcatt ttccacgtcc aattactctt tttgctcttt
    62881 atttgggcgt agtttcactt tcttaacttg tatcgtcttc catcaactcc ttaatcatct
    62941 tcttagcttt attggctcgg tttccttgta aaaggtatct acaaatagtg acaatctgta
    63001 ttaaatcttc atcattttct gtaatgtcaa taatttctat attctgtacc atatattgca
    63061 aagggtttca ctaagttcat aaccgaagcg aattaaacaa cttttgtata gaataactac
    63121 cttttctatt tttccattgg taattctatc tattaattgg tttaatcctt ttgttgtagt
    63181 tgattccact accaagatga ttctctcgaa ttttaaagct tgaagatgta ttgctatttt
    63241 tgctaactgg cgtatttgca actatttttc ccttctattc ttccttcatt gattcccctc
    63301 taaaattact atccataaaa aattgcataa atgacaatag tatttatatt agtcatactg
    63361 ctttttaaca cagcaaattc cagaaaaaca ttcgttacat atttgtgaat ttttccaaaa
    63421 aataacattt ttataaaacg acgaaaaata aatcactacc agtcaacttc tataatatta
    63481 ccaaataaca a
//
LOCUS       DAJSNV010000005        61487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSNV010000005 DAJSNV010000000
VERSION     DAJSNV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.005"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            304..735
                     /locus_tag="ON516_000247"
     CDS             304..735
                     /locus_tag="ON516_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3343764.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            742..975
                     /locus_tag="ON516_000248"
     CDS             742..975
                     /locus_tag="ON516_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3343765.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            1399..1599
                     /locus_tag="ON516_000249"
     CDS             1399..1599
                     /locus_tag="ON516_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3343766.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            1683..2876
                     /locus_tag="ON516_000250"
     CDS             1683..2876
                     /locus_tag="ON516_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3343767.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3054..3284
                     /locus_tag="ON516_000251"
                     /pseudo
     CDS             3054..3284
                     /locus_tag="ON516_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3412..4095
                     /locus_tag="ON516_000252"
     CDS             3412..4095
                     /locus_tag="ON516_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3343768.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4404..4477
                     /locus_tag="ON516_000253"
     tRNA            4404..4477
                     /locus_tag="ON516_000253"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4438..4440,aa:Arg,seq:tct)
     gene            4636..4995
                     /locus_tag="ON516_000254"
     CDS             4636..4995
                     /locus_tag="ON516_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR3343769.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            5118..6749
                     /locus_tag="ON516_000255"
     CDS             5118..6749
                     /locus_tag="ON516_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3343770.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6811..7452)
                     /locus_tag="ON516_000256"
     CDS             complement(6811..7452)
                     /locus_tag="ON516_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3343771.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    7638..7714
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            7734..8042
                     /gene="rplU"
                     /locus_tag="ON516_000257"
     CDS             7734..8042
                     /gene="rplU"
                     /locus_tag="ON516_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3343772.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            8069..8413
                     /locus_tag="ON516_000258"
     CDS             8069..8413
                     /locus_tag="ON516_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3343773.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            8426..8719
                     /gene="rpmA"
                     /locus_tag="ON516_000259"
     CDS             8426..8719
                     /gene="rpmA"
                     /locus_tag="ON516_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3343774.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            9153..11960
                     /locus_tag="ON516_000260"
     CDS             9153..11960
                     /locus_tag="ON516_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3343775.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            12151..12642
                     /locus_tag="ON516_000261"
     CDS             12151..12642
                     /locus_tag="ON516_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3343776.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12745..13734
                     /gene="manX"
                     /locus_tag="ON516_000262"
     CDS             12745..13734
                     /gene="manX"
                     /locus_tag="ON516_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3343777.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13767..14570
                     /locus_tag="ON516_000263"
     CDS             13767..14570
                     /locus_tag="ON516_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3343778.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            14591..15505
                     /locus_tag="ON516_000264"
     CDS             14591..15505
                     /locus_tag="ON516_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3343779.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            15588..15962
                     /locus_tag="ON516_000265"
     CDS             15588..15962
                     /locus_tag="ON516_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3343780.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            16101..16346
                     /locus_tag="ON516_000266"
     CDS             16101..16346
                     /locus_tag="ON516_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3343781.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            16518..17582
                     /locus_tag="ON516_000267"
     CDS             16518..17582
                     /locus_tag="ON516_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3343782.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17828..18253
                     /locus_tag="ON516_000268"
     CDS             17828..18253
                     /locus_tag="ON516_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3343783.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            18246..18695
                     /gene="nusB"
                     /locus_tag="ON516_000269"
     CDS             18246..18695
                     /gene="nusB"
                     /locus_tag="ON516_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3343784.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18792..19646
                     /locus_tag="ON516_000270"
     CDS             18792..19646
                     /locus_tag="ON516_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3343785.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            19647..20984
                     /locus_tag="ON516_000271"
     CDS             19647..20984
                     /locus_tag="ON516_000271"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3343786.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20989..21213
                     /locus_tag="ON516_000272"
     CDS             20989..21213
                     /locus_tag="ON516_000272"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3343787.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            21215..22105
                     /locus_tag="ON516_000273"
     CDS             21215..22105
                     /locus_tag="ON516_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3343788.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            22114..22932
                     /locus_tag="ON516_000274"
     CDS             22114..22932
                     /locus_tag="ON516_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3343789.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            23039..23488
                     /locus_tag="ON516_000275"
     CDS             23039..23488
                     /locus_tag="ON516_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3343790.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            23506..25188
                     /gene="recN"
                     /locus_tag="ON516_000276"
     CDS             23506..25188
                     /gene="recN"
                     /locus_tag="ON516_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3343791.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(25243..25359)
                     /locus_tag="ON516_000277"
     CDS             complement(25243..25359)
                     /locus_tag="ON516_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3343792.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(25432..26388)
                     /locus_tag="ON516_000278"
     CDS             complement(25432..26388)
                     /locus_tag="ON516_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3343793.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            26559..26927
                     /locus_tag="ON516_000279"
     CDS             26559..26927
                     /locus_tag="ON516_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3343794.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            27097..27528
                     /gene="mraZ"
                     /locus_tag="ON516_000280"
     CDS             27097..27528
                     /gene="mraZ"
                     /locus_tag="ON516_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3343795.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            27549..28508
                     /gene="rsmH"
                     /locus_tag="ON516_000281"
     CDS             27549..28508
                     /gene="rsmH"
                     /locus_tag="ON516_000281"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3343796.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            28513..28914
                     /gene="ftsL"
                     /locus_tag="ON516_000282"
     CDS             28513..28914
                     /gene="ftsL"
                     /locus_tag="ON516_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3343797.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28915..31107
                     /locus_tag="ON516_000283"
     CDS             28915..31107
                     /locus_tag="ON516_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3343798.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            31133..32095
                     /locus_tag="ON516_000284"
     CDS             31133..32095
                     /locus_tag="ON516_000284"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3343799.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREKGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            32109..33470
                     /locus_tag="ON516_000285"
     CDS             32109..33470
                     /locus_tag="ON516_000285"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3343800.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            33496..34584
                     /gene="murG"
                     /locus_tag="ON516_000286"
     CDS             33496..34584
                     /gene="murG"
                     /locus_tag="ON516_000286"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3343801.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            34599..35819
                     /locus_tag="ON516_000287"
     CDS             34599..35819
                     /locus_tag="ON516_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3343802.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35975..37300
                     /gene="ftsA"
                     /locus_tag="ON516_000288"
     CDS             35975..37300
                     /gene="ftsA"
                     /locus_tag="ON516_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3343803.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            37323..38564
                     /gene="ftsZ"
                     /locus_tag="ON516_000289"
     CDS             37323..38564
                     /gene="ftsZ"
                     /locus_tag="ON516_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3343804.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            38581..39258
                     /locus_tag="ON516_000290"
     CDS             38581..39258
                     /locus_tag="ON516_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3343805.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            39272..39871
                     /locus_tag="ON516_000291"
     CDS             39272..39871
                     /locus_tag="ON516_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3343806.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39886..40173
                     /locus_tag="ON516_000292"
     CDS             39886..40173
                     /locus_tag="ON516_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3343807.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            40222..41004
                     /locus_tag="ON516_000293"
     CDS             40222..41004
                     /locus_tag="ON516_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3343808.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            41025..41732
                     /locus_tag="ON516_000294"
     CDS             41025..41732
                     /locus_tag="ON516_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3343809.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41994..44792
                     /gene="ileS"
                     /locus_tag="ON516_000295"
     CDS             41994..44792
                     /gene="ileS"
                     /locus_tag="ON516_000295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3343810.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44841..46367)
                     /locus_tag="ON516_000296"
     CDS             complement(44841..46367)
                     /locus_tag="ON516_000296"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3343811.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(46382..47029)
                     /locus_tag="ON516_000297"
     CDS             complement(46382..47029)
                     /locus_tag="ON516_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3343812.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(47213..47542)
                     /locus_tag="ON516_000298"
     CDS             complement(47213..47542)
                     /locus_tag="ON516_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3343813.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47719..48447
                     /gene="sfsA"
                     /locus_tag="ON516_000299"
     CDS             47719..48447
                     /gene="sfsA"
                     /locus_tag="ON516_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3343814.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            48463..49476
                     /locus_tag="ON516_000300"
     CDS             48463..49476
                     /locus_tag="ON516_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3343815.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            49476..50753
                     /locus_tag="ON516_000301"
     CDS             49476..50753
                     /locus_tag="ON516_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3343816.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50816..53518
                     /locus_tag="ON516_000302"
     CDS             50816..53518
                     /locus_tag="ON516_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3343817.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            53670..53987
                     /locus_tag="ON516_000303"
     CDS             53670..53987
                     /locus_tag="ON516_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3343818.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            54017..54337
                     /locus_tag="ON516_000304"
     CDS             54017..54337
                     /locus_tag="ON516_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3343819.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            54445..55905
                     /locus_tag="ON516_000305"
     CDS             54445..55905
                     /locus_tag="ON516_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3343820.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55973..56194
                     /locus_tag="ON516_000306"
     CDS             55973..56194
                     /locus_tag="ON516_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343821.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            56225..56407
                     /locus_tag="ON516_000307"
     CDS             56225..56407
                     /locus_tag="ON516_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3343822.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            56407..56820
                     /locus_tag="ON516_000308"
     CDS             56407..56820
                     /locus_tag="ON516_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3343823.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56943..58124
                     /locus_tag="ON516_000309"
     CDS             56943..58124
                     /locus_tag="ON516_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3343824.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(58195..58359)
                     /locus_tag="ON516_000310"
     CDS             complement(58195..58359)
                     /locus_tag="ON516_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343825.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            58513..58586
                     /locus_tag="ON516_000311"
     tRNA            58513..58586
                     /locus_tag="ON516_000311"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58547..58549,aa:Arg,seq:cct)
     gene            complement(58655..59794)
                     /locus_tag="ON516_000312"
     CDS             complement(58655..59794)
                     /locus_tag="ON516_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3343826.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(60093..60728)
                     /locus_tag="ON516_000313"
     CDS             complement(60093..60728)
                     /locus_tag="ON516_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343827.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(60841..61476)
                     /locus_tag="ON516_000314"
     CDS             complement(60841..61476)
                     /locus_tag="ON516_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3343828.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        20035 a        10597 c        13241 g        17614 t
ORIGIN      
        1 gcaccccttt taattagatt tctagtctaa cttttggggt gcacatcacc acagggctat
       61 tccggttatg agtatgtact ataccatgta aatcagcagc atagcaaagc attttctcgc
      121 ccggtaggtt tgcgacctgc cgggcgattt ttgtcgtctt ttgcggcccg gctgtcccca
      181 aaaaaatttt ttgagaaatt ttcaaaaagg aggtatttag cgggtagctg gcggccttgt
      241 caaagtcttg ttagcggaaa gggagagaac cacctaagtc ccccacagaa agggggtgag
      301 aagatggatg ctatcccccg cgactatgaa gcgcggtgca tgttcgatgc tttttgcaag
      361 acggtactgc gtaacgaggc caaaagctac ttggctgaaa tgaagcgccg ccgtgaccgt
      421 gaagtgtcac taagctcttt gtcacaggca gatttggaca agctctgtac agtagatcac
      481 taccccagcg acagctttac gttttcgtcg catggctatg acctacatat caacaatgag
      541 cttgttgccg aagcctttgc cgcgctgccc agcatggagc agagtatttt aatcctgcac
      601 tgtgttctgg atatggcaga tggtgagatc ggcggccttg tgggaatgtc ccggagtgcc
      661 gttcagcggc acaggacaaa taccttatcg gagctgcgga aacagctaaa ggcgttgatg
      721 ccgaagggag gttagacgcg gatgaagaac ccgaactaca agggcattga gcttttgccc
      781 ctttcggtga tcgaggccgc ccgcgctggg gatgccggag ccgtagagcg cgttttgcag
      841 tattacagcg gttacatgaa caagctgtgt acccggacgc tctatgacga aaacggctat
      901 ccgcatgtgt gtctggacga ggccatgaag cgccgtctgg aaatcaagct catccacgct
      961 atcgtcacga actgacgaac cggccccggc agcatgaaac ggcttaccct ttccccgttt
     1021 cggtgttttc ccggtggcct gatcgcacct tgataaatat gcccgcaaga taacggcggc
     1081 gcatcatagg gcatacggac acattctgtc tgtgccgagc cggacggctg gtgcgccatg
     1141 atacttttcc ctacgggaaa agcggagcga ggaacaccac gccgcaatgt gagcgtggcg
     1201 gcttgcgggc gacgacgtac aggcaggact atgatactcc cttatagcca cagtccgagc
     1261 gttaaaagcg tcgcaggcaa tgggtagggc cgcgtgagaa ccacgcgggg gtgaaagtcc
     1321 cgtggagctg tgccagcagc cgtccgtcta tgcccctaaa tcacacaaac ctttgaattt
     1381 gtgaaagggg gctgcattat gaaccatgat gtgcctattt gggaaaaata cacgctgacg
     1441 attgaggaag cgtctaaata tttccgtatc ggagaaaaca aattgcgtaa gctggccgag
     1501 gaaaatccgg cctctggctg ggtcattatg aatggcaacc gcatccagat caaacgcaag
     1561 cagtttgaga aaataattga cactttggac acaatctaat agtgaaaaag ggccgcagat
     1621 atgttataat aatttataag catatatgcg gctctttccg tcatggaaag gagcatgacg
     1681 agatgtcaga aaaaagacga gacagcaaaa atcgcgtttt gcggtctgga gagagccaga
     1741 gaaaagacgg acgatatgct tacaaatatg ttgatacctt tggaaaaccg cagtttgttt
     1801 actcatggaa gttagtgcct acggataaga ccccggcagg taaacgggag gatatttcac
     1861 tccgggaaaa ggagaaagaa atccagaaag accttgacga cgggatagac accatcggca
     1921 agaaaatgac cgtctgccag ctctacgcca agcagatcag gcaccggggc aatgtgaagc
     1981 acaacaccaa actgggccgg gaacggctga tgcgtattct ggaacaagat cggctgggga
     2041 gctgcccgat tgacagtgtg aagctgtccg acgcaaagga atgggcgctt cggatgaaag
     2101 aaaagggcct ttcttataag accatcaata acgacaagcg ttccctgaaa gcggcttttt
     2161 acaccgccat acaggacgat tgcatccgaa aaaacccgtt tgacttccag cttagtgacg
     2221 ttctggacga cgatacggaa ccaaaggtgc ctcttacacc agcacaggaa gaaagttttc
     2281 tgtcctttat tcaaggtgat aaggtttacc aaaagcatta tgacgcaatc gttattctgc
     2341 tggggacagg gcttcgcatt tcggaactgt gcggactgac ggacaaagat ttggattttg
     2401 aaaaccgggt aatcatcgtg agccaccagc ttttgagaaa taccggcgtc ggttactata
     2461 ttgacgaacc caagacccaa agcggcgtca gaaaaatccc catgaatgag gaagtgtatc
     2521 aggcgttcca gcgagtgata aaaaaccgca aaggtgcaaa gccgttcatt attgacgggt
     2581 atgccaattt cctgtttctc aaacagaacg gctatcccat gacggcggta gattatggcg
     2641 ggatgtttgg caggcttgtg aagaagtaca acaagagcca tgaagaagcg ttgccgaaaa
     2701 cgacgacgcc ccatgctatg cgtcatacat tctgcacccg gttagccaat gcgggaatga
     2761 accccaaagc cttgcagtac attatgggac attccaatat caccatgacg ctgaactatt
     2821 atgcacacgc cacattcgat tccgcaaggg cagaaatgga acggctggca gcctgacaca
     2881 aggccggttt tactactcca tttactactt ttgaggggga aatggtgaga ggtaataaga
     2941 acatttgtaa gtatctaaca atatcaaaaa tgccggaaaa gcccggaaac acgggctata
     3001 ccgacattca agaacttcta aaaagataat ctaaaatccc atataatttt tcctcaaaag
     3061 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
     3121 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
     3181 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
     3241 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
     3301 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
     3361 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
     3421 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
     3481 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
     3541 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
     3601 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
     3661 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
     3721 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     3781 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     3841 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     3901 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     3961 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     4021 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     4081 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     4141 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     4201 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     4261 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     4321 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     4381 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     4441 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     4501 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     4561 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     4621 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     4681 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     4741 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     4801 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     4861 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     4921 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     4981 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
     5041 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
     5101 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
     5161 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
     5221 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
     5281 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
     5341 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
     5401 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
     5461 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
     5521 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
     5581 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
     5641 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
     5701 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
     5761 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
     5821 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     5881 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     5941 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     6001 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     6061 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     6121 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     6181 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     6241 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     6301 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     6361 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     6421 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     6481 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     6541 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     6601 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     6661 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     6721 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     6781 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     6841 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     6901 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     6961 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     7021 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     7081 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     7141 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     7201 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     7261 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     7321 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     7381 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     7441 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     7501 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     7561 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     7621 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     7681 gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg
     7741 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     7801 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     7861 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     7921 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     7981 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     8041 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     8101 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     8161 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     8221 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     8281 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     8341 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     8401 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     8461 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     8521 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     8581 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     8641 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     8701 agtagctcaa gaagcttaat tgaatcttat gattaaagag gttgtgacaa gacttttgtc
     8761 acagcctttt ttgtttataa aaataaaagt tgtagtggag gctacgaagt ttactttgtc
     8821 tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata actatcgttt
     8881 tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt aaacagtgtt
     8941 ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt aaattgaaca
     9001 tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg acgaaacaga
     9061 aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga aaaggagcga
     9121 taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa accgctcatt
     9181 taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa gtgttaggga
     9241 ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga aaactgataa
     9301 agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac gtaaagccag
     9361 ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc
     9421 aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc atgaaaaatt
     9481 cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat tgtttgatta
     9541 caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat tttgcaaaag
     9601 cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct gattatgcca
     9661 ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct tttacaggcg
     9721 cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg tttttggatg
     9781 agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg gatcatggaa
     9841 cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg
     9901 cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt atcccaatca
     9961 atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa
    10021 tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca gaagatgtag
    10081 caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag tcaaatatcc
    10141 agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc tcgatcacaa
    10201 tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc aaccgcagtg
    10261 caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca aatgatacga
    10321 tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat atcatcggag
    10381 ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat catttcattt
    10441 ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt tcttttaatg
    10501 cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc aatcagattt
    10561 atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt tatgctatga
    10621 gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg
    10681 ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg aaagaagtgc
    10741 gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac tattacttga
    10801 ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg
    10861 cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag ttgttagatg
    10921 cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca gcattggacc
    10981 gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc ttagtagata
    11041 tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta
    11101 ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag
    11161 ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat
    11221 cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta
    11281 tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa
    11341 acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag
    11401 aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga
    11461 atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt
    11521 tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag
    11581 agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta
    11641 gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg
    11701 gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac
    11761 gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg
    11821 atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa
    11881 aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa
    11941 aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg
    12001 tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt
    12061 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
    12121 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
    12181 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
    12241 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
    12301 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
    12361 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
    12421 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
    12481 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
    12541 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
    12601 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
    12661 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
    12721 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
    12781 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
    12841 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
    12901 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
    12961 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
    13021 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
    13081 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
    13141 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
    13201 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
    13261 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
    13321 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
    13381 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
    13441 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
    13501 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
    13561 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
    13621 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
    13681 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
    13741 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
    13801 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
    13861 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
    13921 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
    13981 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
    14041 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
    14101 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
    14161 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
    14221 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
    14281 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
    14341 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
    14401 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
    14461 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
    14521 gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
    14581 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
    14641 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
    14701 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
    14761 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
    14821 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
    14881 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
    14941 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
    15001 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
    15061 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
    15121 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
    15181 tgtacaaaga tgggtatcaa tcaaattctc accagttgta tcaactgtaa aattagataa
    15241 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
    15301 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
    15361 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
    15421 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
    15481 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
    15541 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
    15601 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
    15661 ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat agacgcaaat
    15721 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
    15781 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
    15841 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
    15901 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
    15961 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
    16021 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
    16081 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
    16141 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
    16201 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
    16261 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
    16321 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
    16381 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
    16441 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
    16501 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
    16561 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
    16621 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
    16681 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
    16741 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
    16801 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
    16861 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
    16921 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
    16981 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
    17041 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
    17101 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
    17161 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
    17221 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
    17281 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
    17341 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
    17401 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
    17461 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
    17521 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
    17581 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
    17641 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
    17701 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
    17761 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
    17821 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
    17881 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
    17941 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
    18001 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
    18061 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
    18121 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
    18181 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
    18241 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
    18301 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
    18361 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
    18421 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
    18481 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
    18541 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
    18601 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
    18661 ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat
    18721 cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg
    18781 ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag
    18841 agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt
    18901 tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa
    18961 aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac
    19021 tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat
    19081 taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag
    19141 acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga
    19201 ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg
    19261 gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac
    19321 tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac
    19381 tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag
    19441 aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta
    19501 agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg
    19561 tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg
    19621 ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa
    19681 cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag
    19741 gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg
    19801 accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc
    19861 caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt
    19921 cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat
    19981 tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc
    20041 tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg
    20101 atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag
    20161 ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga
    20221 tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc
    20281 cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat
    20341 ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca
    20401 ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc
    20461 aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac
    20521 ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc
    20581 agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag caaatttatc
    20641 atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt
    20701 atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa
    20761 tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt
    20821 tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag
    20881 tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg
    20941 gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca
    21001 acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac
    21061 gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc
    21121 caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa
    21181 gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat
    21241 tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag
    21301 aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt
    21361 attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt
    21421 agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat
    21481 ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt
    21541 agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca
    21601 cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca
    21661 aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga
    21721 agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc
    21781 tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga
    21841 acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa
    21901 aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt
    21961 attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat
    22021 catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat
    22081 caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta
    22141 gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt
    22201 ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt
    22261 agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta
    22321 gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt
    22381 gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct
    22441 ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta
    22501 atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa
    22561 gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct
    22621 attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga
    22681 gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa
    22741 gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg
    22801 atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc
    22861 ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt
    22921 gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt
    22981 ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat
    23041 gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa
    23101 gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat
    23161 ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg
    23221 ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa
    23281 agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg
    23341 aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat
    23401 tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt
    23461 gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc
    23521 agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc
    23581 gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta
    23641 gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa
    23701 gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc
    23761 gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt
    23821 tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta
    23881 gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct
    23941 ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa
    24001 tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc
    24061 tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg
    24121 caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa
    24181 attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta
    24241 gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat
    24301 gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat
    24361 atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa
    24421 cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg
    24481 gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg
    24541 gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat
    24601 cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg
    24661 aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga
    24721 caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag
    24781 ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct
    24841 ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat
    24901 acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa
    24961 tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa
    25021 tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa
    25081 acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc
    25141 acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag
    25201 tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga cactcattac
    25261 agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt
    25321 tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa
    25381 ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa
    25441 cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta atagaactag
    25501 ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact
    25561 gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt
    25621 attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa
    25681 ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt
    25741 tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa
    25801 attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata
    25861 aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg
    25921 aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat
    25981 attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa
    26041 cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag
    26101 cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa
    26161 caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc
    26221 tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg
    26281 atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt
    26341 tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct
    26401 gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa
    26461 gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca
    26521 gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta
    26581 tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat
    26641 tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt
    26701 ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct
    26761 ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag
    26821 aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg
    26881 tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa
    26941 gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa
    27001 taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag
    27061 actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat
    27121 aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa
    27181 aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg
    27241 gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt
    27301 gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat
    27361 atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta
    27421 tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa
    27481 gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag
    27541 gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg
    27601 gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata
    27661 gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc
    27721 aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca
    27781 catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg
    27841 tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg
    27901 gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat
    27961 ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct
    28021 atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa
    28081 aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg
    28141 caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa
    28201 atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca
    28261 atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt
    28321 ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat
    28381 tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa
    28441 cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag
    28501 aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa
    28561 ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt
    28621 ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca
    28681 agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc
    28741 agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa
    28801 aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc
    28861 gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt
    28921 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
    28981 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
    29041 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
    29101 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
    29161 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
    29221 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
    29281 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
    29341 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
    29401 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
    29461 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
    29521 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
    29581 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
    29641 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
    29701 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
    29761 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
    29821 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
    29881 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
    29941 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
    30001 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
    30061 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
    30121 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
    30181 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
    30241 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
    30301 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
    30361 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
    30421 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
    30481 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac agcccaaatc
    30541 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
    30601 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
    30661 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
    30721 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
    30781 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
    30841 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
    30901 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
    30961 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
    31021 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
    31081 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
    31141 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
    31201 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgaga agggaccaaa
    31261 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
    31321 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
    31381 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
    31441 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
    31501 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
    31561 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
    31621 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
    31681 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
    31741 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
    31801 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
    31861 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
    31921 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
    31981 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
    32041 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
    32101 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
    32161 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
    32221 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
    32281 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
    32341 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
    32401 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
    32461 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
    32521 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
    32581 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
    32641 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
    32701 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
    32761 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
    32821 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
    32881 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
    32941 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
    33001 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
    33061 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
    33121 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
    33181 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
    33241 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
    33301 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
    33361 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
    33421 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
    33481 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
    33541 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
    33601 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
    33661 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
    33721 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
    33781 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
    33841 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
    33901 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
    33961 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
    34021 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
    34081 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
    34141 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
    34201 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
    34261 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
    34321 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
    34381 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
    34441 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
    34501 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
    34561 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
    34621 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
    34681 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
    34741 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
    34801 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
    34861 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
    34921 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
    34981 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
    35041 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
    35101 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
    35161 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
    35221 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
    35281 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
    35341 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
    35401 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
    35461 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
    35521 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
    35581 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
    35641 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
    35701 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
    35761 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
    35821 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
    35881 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
    35941 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
    36001 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
    36061 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
    36121 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
    36181 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
    36241 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
    36301 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
    36361 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
    36421 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
    36481 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    36541 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    36601 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    36661 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    36721 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    36781 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    36841 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    36901 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    36961 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    37021 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    37081 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    37141 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    37201 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    37261 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    37321 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    37381 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    37441 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    37501 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    37561 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    37621 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    37681 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    37741 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    37801 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    37861 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    37921 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    37981 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    38041 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    38101 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    38161 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    38221 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    38281 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    38341 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    38401 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    38461 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    38521 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    38581 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    38641 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    38701 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    38761 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    38821 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    38881 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    38941 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    39001 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    39061 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    39121 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    39181 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    39241 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    39301 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    39361 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    39421 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    39481 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    39541 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    39601 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    39661 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    39721 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    39781 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    39841 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    39901 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    39961 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    40021 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    40081 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    40141 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    40201 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    40261 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    40321 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    40381 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    40441 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    40501 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    40561 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    40621 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    40681 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    40741 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    40801 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    40861 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    40921 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    40981 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    41041 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    41101 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    41161 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    41221 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    41281 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    41341 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    41401 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    41461 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    41521 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    41581 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    41641 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    41701 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    41761 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    41821 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    41881 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    41941 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    42001 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    42061 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    42121 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    42181 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    42241 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    42301 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    42361 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    42421 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    42481 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    42541 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    42601 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    42661 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    42721 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    42781 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    42841 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    42901 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    42961 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    43021 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    43081 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    43141 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    43201 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    43261 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    43321 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    43381 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    43441 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    43501 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    43561 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    43621 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    43681 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    43741 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    43801 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    43861 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    43921 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    43981 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    44041 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    44101 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    44161 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    44221 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    44281 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    44341 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    44401 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    44461 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    44521 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    44581 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    44641 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    44701 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    44761 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    44821 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    44881 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    44941 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    45001 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    45061 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    45121 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    45181 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    45241 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    45301 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    45361 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    45421 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    45481 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    45541 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    45601 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    45661 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    45721 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    45781 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    45841 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    45901 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    45961 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    46021 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    46081 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    46141 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    46201 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    46261 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    46321 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    46381 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    46441 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    46501 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    46561 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    46621 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    46681 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    46741 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    46801 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    46861 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    46921 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    46981 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    47041 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    47101 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    47161 agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt
    47221 cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag
    47281 aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta
    47341 ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta
    47401 attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt
    47461 ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga
    47521 gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat gagaaaataa
    47581 tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc
    47641 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
    47701 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
    47761 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
    47821 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
    47881 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
    47941 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
    48001 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
    48061 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
    48121 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
    48181 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
    48241 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
    48301 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
    48361 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
    48421 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
    48481 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
    48541 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
    48601 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
    48661 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
    48721 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
    48781 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
    48841 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
    48901 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
    48961 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
    49021 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
    49081 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
    49141 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
    49201 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
    49261 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
    49321 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
    49381 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
    49441 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
    49501 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
    49561 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
    49621 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
    49681 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
    49741 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
    49801 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
    49861 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
    49921 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
    49981 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
    50041 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
    50101 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
    50161 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
    50221 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
    50281 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
    50341 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
    50401 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
    50461 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
    50521 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
    50581 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
    50641 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
    50701 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
    50761 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
    50821 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
    50881 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
    50941 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
    51001 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
    51061 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
    51121 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
    51181 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
    51241 atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat
    51301 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
    51361 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
    51421 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
    51481 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
    51541 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
    51601 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
    51661 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
    51721 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
    51781 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
    51841 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
    51901 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
    51961 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
    52021 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
    52081 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
    52141 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
    52201 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
    52261 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
    52321 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
    52381 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
    52441 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
    52501 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
    52561 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
    52621 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
    52681 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
    52741 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
    52801 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
    52861 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
    52921 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
    52981 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
    53041 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
    53101 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
    53161 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
    53221 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
    53281 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
    53341 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
    53401 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
    53461 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
    53521 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
    53581 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
    53641 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
    53701 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
    53761 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
    53821 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
    53881 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
    53941 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
    54001 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
    54061 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
    54121 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
    54181 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
    54241 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
    54301 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
    54361 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
    54421 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
    54481 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
    54541 atcggcacac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
    54601 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
    54661 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
    54721 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
    54781 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
    54841 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
    54901 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
    54961 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
    55021 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
    55081 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
    55141 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
    55201 gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca
    55261 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggaccaaat
    55321 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
    55381 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
    55441 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
    55501 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
    55561 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
    55621 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
    55681 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
    55741 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
    55801 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
    55861 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
    55921 cgggacgctt taagtttcgt tcctcataat gaattttcta ggaggaactg aaatgattac
    55981 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
    56041 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
    56101 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
    56161 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
    56221 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
    56281 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
    56341 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
    56401 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
    56461 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
    56521 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
    56581 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
    56641 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
    56701 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
    56761 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
    56821 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
    56881 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
    56941 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
    57001 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
    57061 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
    57121 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
    57181 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
    57241 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
    57301 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
    57361 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
    57421 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
    57481 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
    57541 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
    57601 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    57661 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    57721 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    57781 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    57841 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    57901 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    57961 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    58021 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    58081 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    58141 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    58201 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    58261 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    58321 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    58381 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    58441 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    58501 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    58561 cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg
    58621 gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat
    58681 cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat
    58741 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
    58801 ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt
    58861 gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg
    58921 aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat
    58981 tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat
    59041 caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat
    59101 aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta
    59161 atcctagaag ttcagcaaat cgcattccag tggcaccagc aactaacacg agcataggag
    59221 aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat
    59281 aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc
    59341 tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat
    59401 ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc
    59461 gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt
    59521 tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa
    59581 gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc
    59641 ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa
    59701 atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt
    59761 gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat
    59821 acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt
    59881 tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta
    59941 gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact
    60001 tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct
    60061 gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga
    60121 attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg
    60181 gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt
    60241 agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc
    60301 attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa
    60361 tactcctcgt tatcccatga agcgctgtta attcgaagta aacaattctt ataaagattt
    60421 ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg
    60481 aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg
    60541 cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc
    60601 ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct
    60661 ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta
    60721 ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc
    60781 actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat
    60841 ttatttttta cttactttac tattaatcat atcccattga gcataggatt tttctatgtt
    60901 cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc
    60961 agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc
    61021 attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga
    61081 ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt
    61141 attttgctca gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg
    61201 attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt
    61261 agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga
    61321 tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg
    61381 tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa
    61441 taaaacaata ccagtaaaac caaatatacc gtccattaat agatcct
//
LOCUS       DAJSNV010000006        61084 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSNV010000006 DAJSNV010000000
VERSION     DAJSNV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.006"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..865
                     /locus_tag="ON516_000315"
     CDS             137..865
                     /locus_tag="ON516_000315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3343829.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            865..1119
                     /gene="purS"
                     /locus_tag="ON516_000316"
     CDS             865..1119
                     /gene="purS"
                     /locus_tag="ON516_000316"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3343830.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1121..1792
                     /gene="purQ"
                     /locus_tag="ON516_000317"
     CDS             1121..1792
                     /gene="purQ"
                     /locus_tag="ON516_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3343831.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1793..4015
                     /gene="purL"
                     /locus_tag="ON516_000318"
     CDS             1793..4015
                     /gene="purL"
                     /locus_tag="ON516_000318"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3343832.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4000..5439
                     /locus_tag="ON516_000319"
     CDS             4000..5439
                     /locus_tag="ON516_000319"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3343833.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5462..6514
                     /locus_tag="ON516_000320"
     CDS             5462..6514
                     /locus_tag="ON516_000320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3343834.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6511..7089
                     /gene="purN"
                     /locus_tag="ON516_000321"
     CDS             6511..7089
                     /gene="purN"
                     /locus_tag="ON516_000321"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3343835.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7086..8621
                     /gene="purH"
                     /locus_tag="ON516_000322"
     CDS             7086..8621
                     /gene="purH"
                     /locus_tag="ON516_000322"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3343836.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8637..9911
                     /gene="purD"
                     /locus_tag="ON516_000323"
     CDS             8637..9911
                     /gene="purD"
                     /locus_tag="ON516_000323"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3343837.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10033..10947
                     /locus_tag="ON516_000324"
     CDS             10033..10947
                     /locus_tag="ON516_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3343838.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11080..12645)
                     /locus_tag="ON516_000325"
     CDS             complement(11080..12645)
                     /locus_tag="ON516_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3343839.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12638..14089)
                     /locus_tag="ON516_000326"
     CDS             complement(12638..14089)
                     /locus_tag="ON516_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3343840.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14103..15119)
                     /locus_tag="ON516_000327"
     CDS             complement(14103..15119)
                     /locus_tag="ON516_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3343841.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15339..16427
                     /locus_tag="ON516_000328"
     CDS             15339..16427
                     /locus_tag="ON516_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3343842.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16481..18007
                     /locus_tag="ON516_000329"
     CDS             16481..18007
                     /locus_tag="ON516_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3343843.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18009..19214
                     /locus_tag="ON516_000330"
     CDS             18009..19214
                     /locus_tag="ON516_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3343844.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19471..20448
                     /gene="guaC"
                     /locus_tag="ON516_000331"
     CDS             19471..20448
                     /gene="guaC"
                     /locus_tag="ON516_000331"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3343845.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20712..22136
                     /locus_tag="ON516_000332"
     CDS             20712..22136
                     /locus_tag="ON516_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3343846.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22597..23706
                     /gene="pdhA"
                     /locus_tag="ON516_000333"
     CDS             22597..23706
                     /gene="pdhA"
                     /locus_tag="ON516_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3343847.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23709..24686
                     /locus_tag="ON516_000334"
     CDS             23709..24686
                     /locus_tag="ON516_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3343848.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24711..26354
                     /locus_tag="ON516_000335"
     CDS             24711..26354
                     /locus_tag="ON516_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR3343849.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26362..27768
                     /gene="lpdA"
                     /locus_tag="ON516_000336"
     CDS             26362..27768
                     /gene="lpdA"
                     /locus_tag="ON516_000336"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3343850.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27930..28205
                     /locus_tag="ON516_000337"
     CDS             27930..28205
                     /locus_tag="ON516_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3343851.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28211..28978
                     /locus_tag="ON516_000338"
     CDS             28211..28978
                     /locus_tag="ON516_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3343852.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29146..30975
                     /gene="typA"
                     /locus_tag="ON516_000339"
     CDS             29146..30975
                     /gene="typA"
                     /locus_tag="ON516_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3343853.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31236..33008
                     /gene="recQ"
                     /locus_tag="ON516_000340"
     CDS             31236..33008
                     /gene="recQ"
                     /locus_tag="ON516_000340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3343854.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33122..33424
                     /locus_tag="ON516_000341"
     CDS             33122..33424
                     /locus_tag="ON516_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3343855.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33427..34590
                     /locus_tag="ON516_000342"
     CDS             33427..34590
                     /locus_tag="ON516_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3343856.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34616..38044
                     /locus_tag="ON516_000343"
     CDS             34616..38044
                     /locus_tag="ON516_000343"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3343857.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38065..39189
                     /locus_tag="ON516_000344"
     CDS             38065..39189
                     /locus_tag="ON516_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343858.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39179..39616
                     /locus_tag="ON516_000345"
     CDS             39179..39616
                     /locus_tag="ON516_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3343859.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39600..39938
                     /locus_tag="ON516_000346"
     CDS             39600..39938
                     /locus_tag="ON516_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3343860.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40057..40617
                     /gene="rsmD"
                     /locus_tag="ON516_000347"
     CDS             40057..40617
                     /gene="rsmD"
                     /locus_tag="ON516_000347"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3343861.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40614..41105
                     /gene="coaD"
                     /locus_tag="ON516_000348"
     CDS             40614..41105
                     /gene="coaD"
                     /locus_tag="ON516_000348"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3343862.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41095..42138
                     /locus_tag="ON516_000349"
     CDS             41095..42138
                     /locus_tag="ON516_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3343863.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42297..43025
                     /locus_tag="ON516_000350"
     CDS             42297..43025
                     /locus_tag="ON516_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3343864.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43141..43386
                     /locus_tag="ON516_000351"
     CDS             43141..43386
                     /locus_tag="ON516_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3343865.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43383..43601
                     /locus_tag="ON516_000352"
     CDS             43383..43601
                     /locus_tag="ON516_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3343866.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43737..44276
                     /locus_tag="ON516_000353"
     CDS             43737..44276
                     /locus_tag="ON516_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3343867.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44269..46542
                     /locus_tag="ON516_000354"
     CDS             44269..46542
                     /locus_tag="ON516_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3343868.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46580..47626
                     /gene="holA"
                     /locus_tag="ON516_000355"
     CDS             46580..47626
                     /gene="holA"
                     /locus_tag="ON516_000355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3343869.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47715..47969)
                     /locus_tag="ON516_000356"
     CDS             complement(47715..47969)
                     /locus_tag="ON516_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3343870.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48369..48638)
                     /locus_tag="ON516_000357"
     CDS             complement(48369..48638)
                     /locus_tag="ON516_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343871.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49108..49563)
                     /locus_tag="ON516_000358"
     CDS             complement(49108..49563)
                     /locus_tag="ON516_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3343872.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49701..51629)
                     /locus_tag="ON516_000359"
     CDS             complement(49701..51629)
                     /locus_tag="ON516_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3343873.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51826..52659)
                     /locus_tag="ON516_000360"
     CDS             complement(51826..52659)
                     /locus_tag="ON516_000360"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3343874.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52851..53555)
                     /locus_tag="ON516_000361"
     CDS             complement(52851..53555)
                     /locus_tag="ON516_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3343875.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53578..54204)
                     /locus_tag="ON516_000362"
     CDS             complement(53578..54204)
                     /locus_tag="ON516_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3343876.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54530..56905
                     /locus_tag="ON516_000363"
     CDS             54530..56905
                     /locus_tag="ON516_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3343877.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56929..57420)
                     /locus_tag="ON516_000364"
     CDS             complement(56929..57420)
                     /locus_tag="ON516_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3343878.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57607..58527
                     /locus_tag="ON516_000365"
     CDS             57607..58527
                     /locus_tag="ON516_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3343879.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58681..59025
                     /locus_tag="ON516_000366"
     CDS             58681..59025
                     /locus_tag="ON516_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3343880.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59215..59715)
                     /locus_tag="ON516_000367"
     CDS             complement(59215..59715)
                     /locus_tag="ON516_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3343881.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60243..60326
                     /locus_tag="ON516_000368"
     tRNA            60243..60326
                     /locus_tag="ON516_000368"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60277..60279,aa:Leu,seq:cag)
     gene            complement(60650..>61084)
                     /locus_tag="ON516_000369"
     CDS             complement(60650..>61084)
                     /locus_tag="ON516_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3343882.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20052 a        10552 c        13104 g        17376 t
ORIGIN      
        1 acattaaaag aatctaatac cttaaatgta catatttttg ttgatttata aaaagatgat
       61 tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt aatgtaaata
      121 aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag ccaaaaaaat
      181 atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag caactgcgtt
      241 gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc aaatcacaag
      301 tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg aacaattgtc
      361 taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg ttatccgaaa
      421 tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag aattcatcaa
      481 accaatcatt gaattctact ataaggatga ccagttggat gatccattta tcaatgaaga
      541 gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc agtgagctga aagcccaagc
      601 gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg ttttagtaga
      661 tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg aagtaacacc
      721 ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag acgtttaccg
      781 cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc tatcggaact
      841 aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac ctataaagat
      901 tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat gggatatgaa
      961 acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga tgcagaagaa
     1021 ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt aaatatggaa
     1081 acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg cggtcatcgt
     1141 ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga tcatgggcgc
     1201 tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg gggttttact
     1261 gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc ggttttctac
     1321 aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg gtacatgcaa
     1381 tggattccag atattgacag aagcaggact tttgccaggt gtactgagac gaaatgagtc
     1441 tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg cctttacttc
     1501 agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag gaaattattt
     1561 ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct ttacttataa
     1621 tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag cagggaatgt
     1681 gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat cagaagatgg
     1741 gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat agatgaaaac
     1801 attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg aatggggatt
     1861 gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc ctaattatac
     1921 ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa attctaaacc
     1981 ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg gagaaggcgc
     2041 aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa gtcacaatca
     2101 tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa tcatcagaga
     2161 tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt tcggcgaatt
     2221 agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg gcggatacgg
     2281 caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt gttatgaagg
     2341 aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata tccagaaagg
     2401 gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag gtcgcgacgg
     2461 tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac agcaacgttc
     2521 tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt gtttggaatt
     2581 aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag caggacttgt
     2641 ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga cattagatga
     2701 agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg aatcacaaga
     2761 acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac tatttcagaa
     2821 gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat atcggctata
     2881 tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag atgcaccagt
     2941 ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt tagctcctta
     3001 tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc aaccgacgat
     3061 tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca atacagttgt
     3121 ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag cgttagctat
     3181 gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag gacaaatagc
     3241 agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg cgatcaccga
     3301 ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat cgacttcagc
     3361 tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag gaaatgtctc
     3421 tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg gtatggtcgg
     3481 attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag gggatctaat
     3541 ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa agatgcagct
     3601 tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga gaaaccaaga
     3661 gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact gttcagaagg
     3721 tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga tatctgtaca
     3781 gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg tgttgacagt
     3841 cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg ttttagcagg
     3901 taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga tcaaaataga
     3961 aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga agtgaagagt
     4021 ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc tagtgtggca
     4081 tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat cgtgtccaat
     4141 gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt attcaaagat
     4201 caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg ttatgcaaca
     4261 gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca tgatcagtcg
     4321 atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa agagttagag
     4381 gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca tttgatccga
     4441 cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga agtcaaaggt
     4501 ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga cccaaatggt
     4561 tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc ctcggaaact
     4621 tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg tgagatagtg
     4681 atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca gctttctatt
     4741 tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg gatcaatgtg
     4801 catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt aaaagcagat
     4861 atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc ggaagctagt
     4921 ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac atttatccag
     4981 ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt acgaggagta
     5041 gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac gacaagcaga
     5101 cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag gattgcttct
     5161 cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa agaactcatt
     5221 gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc tttaggtttt
     5281 ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc gtattcagga
     5341 ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta tgaagaaaaa
     5401 tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa gaagggagta
     5461 tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag ctggatatga
     5521 agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta tgatgggcgc
     5581 tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag agccagtcct
     5641 tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag cagacaaaca
     5701 cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag cacaaggcgc
     5761 agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt tacgtttgga
     5821 aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc tgattggcgg
     5881 agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag ccggatttgc
     5941 tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc caggagatgt
     6001 gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg ttcgcaaaat
     6061 tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga aaaaaacact
     6121 tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc cgctgatcaa
     6181 agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag aaaatatccc
     6241 gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc cgattcttcc
     6301 aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt atgaaatctt
     6361 caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag aaatagtaca
     6421 cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg ccaaagaaaa
     6481 acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc ttctggaaac
     6541 ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga atcttcgatt
     6601 gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac tgcgttgagt
     6661 gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta tgaagaagca
     6721 attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg atatatgcga
     6781 atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat ccatccgtct
     6841 cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc tggagtaaaa
     6901 gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc aatcatccgg
     6961 caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga aaaaatccat
     7021 agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa tggaggctat
     7081 ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta gcattcgccc
     7141 aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag catgcgttgg
     7201 ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct gaaatgatgg
     7261 atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga agacgcgatc
     7321 tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac tttgtttgtg
     7381 tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata gcagaagcta
     7441 ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa aaccatgaat
     7501 ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa ttacgtcagt
     7561 atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt cgtcatactg
     7621 cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa aaagaaccgg
     7681 aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa aatagccatc
     7741 aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca gcagcaaaac
     7801 aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct gcgctacgga
     7861 tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat ccttgtggta
     7921 ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca gatcctgttt
     7981 caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca gcagaacaga
     8041 tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa gctttggaat
     8101 tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag gaagaaaatc
     8161 aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat caagatctgg
     8221 caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact ccagcagaag
     8281 caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat gcgatcgtac
     8341 tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga gtcggttcag
     8401 taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta gctagcgatg
     8461 cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata aaagcaatca
     8521 ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat aaatacgatc
     8581 tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc ggataaatga
     8641 acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa cttttagcca
     8701 gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca aaagatggta
     8761 tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt gttaagaaag
     8821 aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg atcgtcgatg
     8881 attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct ctgatcgaag
     8941 gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca gctaaatcca
     9001 gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt gcgccaatcg
     9061 tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc acagtgaagg
     9121 aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat agtagcaaaa
     9181 aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg tttgtccgta
     9241 ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt gataacgata
     9301 aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt tcaaaaaaga
     9361 tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg aaagaactag
     9421 gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa ggaccaaaag
     9481 tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt tctcggttga
     9541 agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta gagttaaagt
     9601 ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac cccgaagaat
     9661 atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt tactatgctg
     9721 gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat ttagtagaag
     9781 catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta atggataaaa
     9841 cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat agcgaactga
     9901 tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa tcggaagcgc
     9961 cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt atataaataa
    10021 ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc agctgcagtg
    10081 atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat tgagacagct
    10141 tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg tgaatgtgaa
    10201 gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct ttttattcaa
    10261 acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca tatcattgaa
    10321 tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt acttcttcat
    10381 cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga attggaacgg
    10441 tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat cgaattgctg
    10501 aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt gaaacatact
    10561 ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat cgatcatgac
    10621 ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg gtcaccatac
    10681 cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga attaaaccaa
    10741 acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc ttctgcttgg
    10801 attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt agggcgaatc
    10861 aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta cgaattgtac
    10921 cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa gggctataaa
    10981 ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg tcttttttta
    11041 ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt tgtttttaag
    11101 gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa ataaataact
    11161 gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac gatagtctga
    11221 ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat cgatgaatgt
    11281 ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg caacttgttg
    11341 agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa aatccaatgc
    11401 atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag cagttaagaa
    11461 agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg
    11521 ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt tcaggcagac
    11581 atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag ttggtgtgcc
    11641 ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata atgaagacaa
    11701 atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt cttgaatcaa
    11761 atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta tcatctgcgt
    11821 gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa tggcgacaga
    11881 aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta cctgataact
    11941 ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga atcgagagga
    12001 aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat aatcactgag
    12061 atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata cggtcatttt
    12121 tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac tgatcgtaag
    12181 tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa caagatcaga
    12241 agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt ctattgcttg
    12301 tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta cctggattgc
    12361 tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag tccaaggaag
    12421 tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt
    12481 cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac catctccagc
    12541 ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt cccgcaagtt
    12601 attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg tttcctccaa
    12661 ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg tatttgctgt
    12721 caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg tttacgttgt
    12781 aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct ttacgtattt
    12841 ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat tggatcatct
    12901 catcaatttt gcatgcggta atacgaataa atcctttgtg acggacgtac ttcgttccat
    12961 ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa acaggaaaaa
    13021 gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt tttaagatga
    13081 ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct tgtcctttgc
    13141 ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct tcttcaaagc
    13201 agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa tggggagtaa
    13261 tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc aaagcgttct
    13321 cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta agtgtttgta
    13381 attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa agttgttctg
    13441 acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag cgacgattct
    13501 ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt aatagaatga
    13561 gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca cgaacaaaat
    13621 cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg atctcttcat
    13681 tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga taatcttcca
    13741 gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt gtatttgtat
    13801 tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc tcattgaact
    13861 catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact ttttgatcaa
    13921 ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga taaatcaaaa
    13981 gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat agaagcatgg
    14041 ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg atacttctcc
    14101 agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa
    14161 ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg
    14221 gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct
    14281 gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct
    14341 ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta
    14401 tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg
    14461 tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt
    14521 ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat
    14581 ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg
    14641 atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct
    14701 tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata
    14761 tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc
    14821 cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc
    14881 cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa
    14941 tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc
    15001 gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta
    15061 caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact
    15121 ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag
    15181 tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt
    15241 acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta
    15301 gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga
    15361 ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag
    15421 gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa
    15481 gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag
    15541 atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta
    15601 caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt
    15661 tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt
    15721 cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag
    15781 cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat
    15841 ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt
    15901 tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc
    15961 aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa
    16021 gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt
    16081 taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg
    16141 tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa
    16201 cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag
    16261 caatcagtga tggagaagag gttccggtca atgatactga aacttatgat aatggggtga
    16321 aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag
    16381 agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca
    16441 aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa
    16501 aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat
    16561 gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt
    16621 gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga
    16681 tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat
    16741 tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa
    16801 tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact
    16861 gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg
    16921 gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt
    16981 ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg
    17041 agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt
    17101 ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa
    17161 tgaaatgatc agcgaagagc atattattcg cgggatggtg ggcagagatt tagttgatcg
    17221 ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt
    17281 ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa
    17341 aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag
    17401 tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga
    17461 gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga
    17521 ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag
    17581 tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga
    17641 aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct
    17701 tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat
    17761 tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac
    17821 gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc
    17881 tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga
    17941 agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt
    18001 gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga
    18061 aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac
    18121 ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc
    18181 ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct
    18241 taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca
    18301 tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag
    18361 gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta
    18421 tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc
    18481 aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact
    18541 taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg
    18601 tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg
    18661 aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa
    18721 cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg
    18781 ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg
    18841 gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg
    18901 tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct gctcgattga
    18961 atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt
    19021 ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct
    19081 tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc
    19141 aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa
    19201 aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc
    19261 acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa
    19321 tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt
    19381 gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt
    19441 gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa
    19501 ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta
    19561 ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa
    19621 acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa
    19681 gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt
    19741 gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat
    19801 cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt
    19861 cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg
    19921 gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca
    19981 ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca
    20041 gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga
    20101 acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca
    20161 ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat
    20221 aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga
    20281 aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa
    20341 gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt
    20401 gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa
    20461 atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa
    20521 tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat
    20581 cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa
    20641 aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa
    20701 aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta
    20761 tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat
    20821 cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg
    20881 aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc
    20941 agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc
    21001 gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa
    21061 catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt
    21121 ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct
    21181 atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt
    21241 cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa
    21301 cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac
    21361 ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg
    21421 acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc
    21481 cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag
    21541 aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat
    21601 ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg
    21661 tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg
    21721 agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc
    21781 ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg
    21841 taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt
    21901 ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa
    21961 aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc
    22021 tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc
    22081 aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc
    22141 acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt
    22201 ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt
    22261 tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa
    22321 atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat gaaatataaa
    22381 acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt ggcataattt
    22441 cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca tgataattta
    22501 ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg cagaatgaag
    22561 agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa agcaccgatc
    22621 gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca agcacttgat
    22681 caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga tgaactcgtt
    22741 gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac agcgctgaac
    22801 cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag tcagctggct
    22861 agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga tgtacctcaa
    22921 ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg acatgctgca
    22981 ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat cggtgcacaa
    23041 tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa aaatgtcgtc
    23101 ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc aatcaatttc
    23161 gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta tgcgatctct
    23221 acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt cgcagctggg
    23281 attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt atctaaaatg
    23341 gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact gacttatcgt
    23401 tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa agacacagat
    23461 gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac ggaaaaagga
    23521 ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga aatcaaagca
    23581 gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt gaaaaacatg
    23641 ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc gaaggagtcg
    23701 aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta gcacttgaac
    23761 tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac ggaggagttt
    23821 tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc gatactcctt
    23881 tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga tttcgtccag
    23941 ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta gtagctcaaa
    24001 tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca attcgttcgc
    24061 catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa ggtttgatcg
    24121 ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca aaaggactat
    24181 tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg aaactatatc
    24241 gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat aaagcagcag
    24301 ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg cgtgaagcaa
    24361 tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt gatcttcgta
    24421 cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact ggtcgcgtag
    24481 ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt tctgagatct
    24541 cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca cctgatacag
    24601 tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat atcgaagaca
    24661 aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc atggcttatc
    24721 aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg
    24781 tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat
    24841 cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt gttccagaag
    24901 gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga cacgaagaca
    24961 atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac
    25021 ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac
    25081 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
    25141 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
    25201 ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa
    25261 atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca tcgtcaacta
    25321 gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg
    25381 ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc
    25441 gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa
    25501 aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag
    25561 cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac
    25621 cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa
    25681 cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat
    25741 tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg
    25801 ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga
    25861 ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt
    25921 ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa
    25981 atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag
    26041 caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga
    26101 tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag
    26161 tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa
    26221 tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag
    26281 caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa
    26341 tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt
    26401 gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa
    26461 aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt
    26521 ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg
    26581 tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa
    26641 aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt
    26701 gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca
    26761 gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc
    26821 gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg
    26881 aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc
    26941 ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta
    27001 ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta
    27061 acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg
    27121 aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta
    27181 ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa
    27241 cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc
    27301 ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct
    27361 gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc
    27421 ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt
    27481 atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt
    27541 aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg
    27601 caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca
    27661 ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc
    27721 gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac
    27781 aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta
    27841 ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg
    27901 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
    27961 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
    28021 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
    28081 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
    28141 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
    28201 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
    28261 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
    28321 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
    28381 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
    28441 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
    28501 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
    28561 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
    28621 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
    28681 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
    28741 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
    28801 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
    28861 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
    28921 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
    28981 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
    29041 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
    29101 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
    29161 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
    29221 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
    29281 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
    29341 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
    29401 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
    29461 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
    29521 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
    29581 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
    29641 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
    29701 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
    29761 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
    29821 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
    29881 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
    29941 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
    30001 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
    30061 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
    30121 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
    30181 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
    30241 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
    30301 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
    30361 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
    30421 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
    30481 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
    30541 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
    30601 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
    30661 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
    30721 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
    30781 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
    30841 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
    30901 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
    30961 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag
    31021 ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata
    31081 aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag
    31141 tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa
    31201 atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact
    31261 ttggttatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg
    31321 aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag
    31381 ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc
    31441 aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg
    31501 gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata
    31561 tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc
    31621 gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa
    31681 gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt
    31741 tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag
    31801 aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag
    31861 atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta
    31921 tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg
    31981 atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag
    32041 aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta
    32101 taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat
    32161 actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt
    32221 tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag
    32281 ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt
    32341 cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt
    32401 gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc
    32461 tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag
    32521 caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac
    32581 tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg
    32641 gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac
    32701 aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat
    32761 cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat
    32821 cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc
    32881 gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc
    32941 ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg
    33001 ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt
    33061 ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca
    33121 gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat
    33181 caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga
    33241 agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat
    33301 aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa
    33361 tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga
    33421 atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc
    33481 tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta
    33541 ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt
    33601 agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt
    33661 ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt
    33721 aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc
    33781 gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac
    33841 agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt
    33901 actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt
    33961 ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga
    34021 ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta
    34081 cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac
    34141 gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta
    34201 tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc
    34261 gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta
    34321 gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg
    34381 ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat
    34441 cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa
    34501 ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg
    34561 gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa
    34621 aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga
    34681 attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt
    34741 caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga
    34801 tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta
    34861 cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt
    34921 tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc
    34981 agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga
    35041 cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg
    35101 cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga
    35161 acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata
    35221 catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat
    35281 ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc
    35341 gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt
    35401 gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga
    35461 tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat
    35521 tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa
    35581 agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg
    35641 aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta
    35701 tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt
    35761 agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga
    35821 aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac
    35881 aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa
    35941 aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg
    36001 aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag
    36061 tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga
    36121 ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat
    36181 caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct
    36241 gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg
    36301 tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg
    36361 ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac
    36421 gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa
    36481 tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt
    36541 tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg
    36601 taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa
    36661 atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat
    36721 cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag
    36781 tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa
    36841 tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat
    36901 gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt
    36961 caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg
    37021 ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga
    37081 agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc
    37141 tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat
    37201 gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata tggcactatt
    37261 tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt
    37321 ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc
    37381 taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct
    37441 tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta
    37501 tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta
    37561 tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca
    37621 aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat
    37681 tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt
    37741 gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac
    37801 ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt
    37861 tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac
    37921 tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt
    37981 atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa
    38041 ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta
    38101 ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag
    38161 gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa
    38221 gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc
    38281 caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa
    38341 gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta
    38401 ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt
    38461 acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac agaagttgga
    38521 atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact
    38581 tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat
    38641 aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat
    38701 caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg
    38761 actttattaa atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc
    38821 tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga ggaaatattg
    38881 tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa
    38941 ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact
    39001 agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc
    39061 gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt
    39121 tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt
    39181 gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat
    39241 aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc
    39301 aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc
    39361 ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa
    39421 aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca
    39481 aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt
    39541 agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca
    39601 tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct
    39661 acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa
    39721 gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa
    39781 tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga
    39841 attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag
    39901 agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt
    39961 aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt
    40021 atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat
    40081 ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta
    40141 aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt attggatctg
    40201 tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta
    40261 tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa
    40321 gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca
    40381 gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata
    40441 atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta
    40501 tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga
    40561 aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac
    40621 gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac
    40681 gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa
    40741 gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta
    40801 atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg
    40861 cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg
    40921 ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag
    40981 aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga
    41041 gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa
    41101 gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt
    41161 gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa
    41221 ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt
    41281 ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa
    41341 ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa
    41401 cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac
    41461 aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat
    41521 ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa
    41581 agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc
    41641 agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca
    41701 gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca
    41761 gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta
    41821 gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact
    41881 ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca
    41941 agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat
    42001 gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa
    42061 aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat
    42121 ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga gttctgtcac
    42181 aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga aaaaattgac
    42241 gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa ttttaaatga
    42301 aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg atagaaaaaa
    42361 tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg atagttctta
    42421 tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga gaaacagaag
    42481 agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat ccatcagctg
    42541 aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa
    42601 gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat gatgtactaa
    42661 agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga
    42721 tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca gaacatcttg
    42781 atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct cccaaaataa
    42841 atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga tcagtcaaag
    42901 cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg gaagacctgc
    42961 aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc gttacaatta
    43021 aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg gatactaaga
    43081 ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat aggaaatagc
    43141 ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct ccaaaataaa
    43201 atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca agctctacga
    43261 gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat tctcattggg
    43321 acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt
    43381 ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt atcacgccac
    43441 cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa gaatatatct
    43501 ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt atacggaatt
    43561 tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg gacttcattt
    43621 cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt aaagaatatt
    43681 ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt
    43741 tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat aagtcacata
    43801 atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta tctttacgta
    43861 gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg atcattgctg
    43921 gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg tgttacgtcg
    43981 tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta cagtgtgcaa
    44041 aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca tgtttggcct
    44101 gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat gactatcgaa
    44161 atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac caagttgctt
    44221 tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat gtctagcgtt
    44281 ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat gattatctgt
    44341 ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat ttttttatgg
    44401 ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag cataacggta
    44461 tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac atctgcgcaa
    44521 attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat ggaaggaagg
    44581 ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga agcagaattg
    44641 aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg cgagttcatc
    44701 tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt caggaaccat
    44761 agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca aaatcactgg
    44821 cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc tgaatttccg
    44881 caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc aggattcaga
    44941 gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct atcgggtcta
    45001 cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg atggccacta
    45061 cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat tgcaggaagt
    45121 tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt caaattgctg
    45181 aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct tttactattt
    45241 gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag ttttttgttt
    45301 atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt cctgtttact
    45361 atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt aggtagtctt
    45421 tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt gttatttctc
    45481 acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga gggattaatc
    45541 ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg acgtttgccc
    45601 cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag gttgaaagaa
    45661 caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt gactttgcca
    45721 tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt
    45781 ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca gttacacttt
    45841 gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca
    45901 tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca cgacgatacg
    45961 gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa aatttatatc
    46021 ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc tcaaaaaaat
    46081 ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta tgatctttat
    46141 gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg tctatggatg
    46201 gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat ggaaaaacaa
    46261 ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca tcacggtagc
    46321 cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg gattatatcc
    46381 tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac actagaagaa
    46441 aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg ctggcatcca
    46501 gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc atctgctcat
    46561 ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt tacaaaaaat
    46621 acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt ttctccaaga
    46681 tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg acttaaattt
    46741 tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag cagagacgtt
    46801 gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt taacaggtga
    46861 aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt tgaaagaacc
    46921 attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg aacggaaaaa
    46981 agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa
    47041 agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa tggaacgaag
    47101 tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga tgagagagtt
    47161 acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag aagtagaaga
    47221 attagttcct aaaacgttag aacataatat ttttgacatg acccaatata ttttaaaagg
    47281 gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg aagaaacaat
    47341 caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa aatttcttgt
    47401 aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc catatcgtgt
    47461 aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac agttgtttga
    47521 tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag aattaagttt
    47581 tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag aaagacgagg
    47641 gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa aaaaatcggc
    47701 cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc gcggttagct
    47761 ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac agcttcttta
    47821 tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt tttgattgca
    47881 gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct tgtacgaaca
    47941 cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt aagttcttac
    48001 gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg ctttgcaata
    48061 tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt cagtaaaaac
    48121 ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat agcgtcaaac
    48181 caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt tggtactaag
    48241 ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat acgattccct
    48301 gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta tcctttgatc
    48361 agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt ttttatcaac
    48421 atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc cagcacatat
    48481 ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag caggccattg
    48541 atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg ttcttgtaaa
    48601 acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa
    48661 agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt ggatgcgatc
    48721 attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga gccaaaaaga
    48781 aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta ttacaaaaca
    48841 ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta ataggtcaat
    48901 caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata agctaagccc
    48961 agaatcaaaa gtaccgctag aagaaaccaa ccgattgttc ctatgaaata ctttaatttt
    49021 tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg gggtgttaag
    49081 aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt gtaagataca
    49141 gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga agacaacacc
    49201 taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac
    49261 acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc agccaatagg
    49321 ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga tagggattag
    49381 aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa gccaagggaa
    49441 aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg ctatcatcag
    49501 actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa gccaactttc
    49561 catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag aaagttacaa
    49621 aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa aataaacaaa
    49681 atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta tagtggacaa
    49741 gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca
    49801 ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg tagatttaaa
    49861 aattgcagga agttagaagc aatcaacaag agaataacaa gaatcgaaaa aataatattt
    49921 tgtttgatga tccgccgcat ttttaaagat agccgatgac tgtagacaaa tttatttaag
    49981 tcgcttttca ttaagacgat gtctgcgata tccatagcgg catctgtccc ttgccccata
    50041 gcgacaccta aagaagcggc tgctaaagca ggcgcatcat tgactccatc accaaccatc
    50101 gcatttactt ggtaaagttc attctctttt ttgactagtt gtgttttttc ttccggagta
    50161 cagcttgcat agtaatgttc aatccctact tgttcagcaa tggcctttgc tgtgccagaa
    50221 tggtcgccgg ttatcatact tgtatggatg ttttgggatt taaagtagga aattacttgt
    50281 tgggtatttg cttttggaat atctagcaga cctaaaacag caattaactg ctgatcttta
    50341 gaaagataaa tcacggtttt gccttgattt tccaaacgtt cgatcatttc cgcaatctct
    50401 ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc caactttcca ttgcgcacct
    50461 aaataggtag tttgtaatcc aaaaccagta atttcttcta ttggtaagta ttggatttct
    50521 tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag caagtggatg cgtcgttttt
    50581 tgttccatag caacaagaag ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca
    50641 gttacaactg gttttccttt agtgagcgtt cctgttttat caaaggcaat cgcttttaat
    50701 tcagaaagct tttctaaatg gataccgcct ttgattaaaa tcccattttt tgcagaattt
    50761 gaaagagcag ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg agaagccaca
    50821 actaataata ccattcctcg ataaaagctt tcatcccaac tccaacctag gaaaaagtgg
    50881 gggagaagga tcattagagg aacagcaagc aaaatcaatt ttacataatt attttcgatt
    50941 cgttcgataa atgaagctgt ttgagttggc gtattctgcg cttcttctac taattggata
    51001 atcttagcaa agacagtttc ggaactcgtt tttgttactc gaatcgtgat gggatttcct
    51061 tgattcagcg ttcctgcata catctcatcg cctaaatatt tttctacagg cacagattca
    51121 ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga
    51181 acagctgctc ctttagcgac aaataccaag ttatccacct ttagttgaga aacatctact
    51241 tcttgcattt gtccattttc ttttaataat tgggcttttt gtggttgaag attcatcaaa
    51301 gcagtaattt ctttttgact tttattagtt gtgtattcct ctaatgcacc gcttaaacaa
    51361 aagatgaatg tcaacattgc tccttcaaag taattaccga tcaaacaggc accaatagca
    51421 gctaaagcca tcaataaatc cacattcagt gtccgatcat tccacaattc tttcaagcct
    51481 tccgatgttt gtttatatcc accgatgata atggcactga tataaaaaag tggggtgatg
    51541 gtaagagaga atcggtcgaa gataaaaccg aagatcataa ataaaaggca gccgattgtt
    51601 gctgcaaaag cttttttctg attagtcatc gttattagac ctccttgtca aaacaaggat
    51661 agcataataa aaatgtaatt gataataata acagctaaat gagaacgata atggttatca
    51721 tttggtgatt aagcttgatg ttgtttttat tatttataat gattattatt aaagaaaata
    51781 aaaaaccaga acatttctca aataagagaa gtgttctggt tttttctaca ttgcaataaa
    51841 ccaataacca atcaaaatga taccaaggat aatacgatac cagccaaaag cggtaaagtc
    51901 gtttttcttc aaatagctta gtaagaattt gatagctaag acggaaacca caaaagatac
    51961 gaatgttcct acaagaagaa caacagtttc ttccattttg aatgaattcc cttcagctaa
    52021 atatttgacg atcttcaagc cgcttgcgcc aaacatgaca ggaatcccta agaaaaatga
    52081 aaactctgta gcgacaaaac gcgaagcacc aatcaagata gatcctagga tcgttgcgcc
    52141 agaacgggat gttccgggaa caagtgccaa gacttggaat aacccaacaa gaagagctgc
    52201 ttggtacgta aagtcattca gagaagtcca acgaggtgtt ttgtttttgt tgcgtttttc
    52261 gacaataata aaggcaatac cgtaaacgat caaaacaatg gcaactggta agaacttgtg
    52321 gaaatttgct tctaaccaat catccaaagg caaaccaatg atgatggctg gtatgcaagc
    52381 aacgatcact ttaaaccaaa gtgtccacgt atcttttttt tctaccgttg atttacttgg
    52441 agaaaatggg tttaacttgt gaaaataaag aacgacgaca gccaagatcg cccctaattg
    52501 gataacgaca ttgaacatcg acataaattg tgaactggca tccagtttga tgaattcatc
    52561 tgctaggatc aaatgaccag tactactgat gggcagccat tccgttattc cttcaataat
    52621 accgagaatc actgctttga taatatttgc aaaaaacata aattccttcc ttcccaatta
    52681 agatacatct tatagtatac cgttattttt tgagaaaccc aatctgtttt ttaatattaa
    52741 gaaaaacttg agaaataaag cagataagta cacgaagaga aaaaagaaga caaagccctc
    52801 gttcagactt tgccttttat gattctctgg taaaaattaa aatatcccca ttatagaaac
    52861 gagtcgcgac tatgtgtttt cccgttatcg atataagacg tatcattgat gccgtggata
    52921 gaaaatcgac cgtttgcata agtgaccttt gttacgctgc catttaaaag cccagcacga
    52981 acaggctgtt taggatcgat cagattaagc aaaaaggcga tcgtcaaacc atgagatacg
    53041 actaaaacat ttccaccacc atttttttcg cagtgatggg cgatatcttc aaatcctgtc
    53101 catacacggt ctcgcaatgt ttcataagtt tccgcccacc cagcggtatc taagttgtat
    53161 acagcattag caatttgttc aaaagaaact tctgtggtaa acatttcatc atatgtttta
    53221 aaattcaata cacgagggat cacaccccac atttccatgt catatccacc ttcaagagac
    53281 ccgaaacacc attctctaat ccttttttca ataaaataag gaatttcttt tcccttagaa
    53341 tgttctccta gaataatacg ggcagtttcg attgcacgac cactgtcact agaataagca
    53401 aatgaaaagt cgacattttt taacccgcga cctaaataat gaatgccttc ttgtccttgc
    53461 tttgtgaggg gtgtatcaca ccagccttgt actcgttcaa ttgtattgaa catcgttttt
    53521 ccgtgtcgta cgatataaag tgtagtttct gtcattttat tctcctccta agtcgtttta
    53581 acgattgaaa aaaggatttg tccttttttc ctgttcaatc gtcgttgcat cgccatgtcc
    53641 tggatagact gcaaattcat caggtaatac aaacagctgg gtccgaatac tatgaagtaa
    53701 ttgttctaaa ttccctgtat acagatcggt tctaccgata cttcctctaa acagggcgtc
    53761 gccagaaacg acaaaatcat caaaaataaa gctgacgctt cctatagaat gccctggggt
    53821 agggacgacg cgaaaagaca tgtttcctaa agtataagtt ttcatttcaa attcatattc
    53881 tgcaggcgag acaataacat cagcaatatc atcatgtcta ccgagtccag aaagattcaa
    53941 aatcggatca cctagccatt tttgctctag tgggctgaca taaactggga tttgataata
    54001 cgcgcgtaaa ggttccactg caccgatatg atcgtagtga gtatgggtaa gcaaaatcgc
    54061 taccggctgt tggtttgttt ttttgatttc ttgtttgatt cgttccgcat cttctccagg
    54121 gtcaatgatc aaaagtgctt cttcgttgta gaccaaataa cagttttctt caatcattcc
    54181 tgtttttaat ctttcgatat gcatgtgcat tcctccttga tagccaaaac ttctttcatc
    54241 cattagtata acgcattcat tgctaggaaa caaaaacagg tacttatttc tctttactta
    54301 tctgaattat cagactatat gaaaagtatt tggatattct aaagagaagg aaagtaagtt
    54361 ttcatagcat tttaatatgt ccttgttaca ttagattcat ccaaaaatcc aaattctata
    54421 tcctatatcc ctgcctaatc agcagggtgt ttttttatgt ttggaaagag agctcttttt
    54481 gctaaactag gttttttcag gtaatcttag agagaaagag gtggtggaaa tgatcaaaca
    54541 gtcaaaaata tctgtgcggc acttagtaga atttattttg cgacgtggaa gtatcgacaa
    54601 tcgtaaaaaa agtaaccata ctgcattaga aggagcgaga atccaccgca agctccaaaa
    54661 agatgcggga gaaaactatg aaaaagaagt ctttttaaaa acaacagttc aattagatga
    54721 atattacctg atcgttgaag gccgtgcaga cggtattttc aaaagagacg gtatttatta
    54781 tatagatgaa atcaaaacct ccgaaccacg atttgaagat ttagaaccag aacaagtgga
    54841 acttttcttt catcaagcaa gggtgtatgc ctatatctat agtcaagaac atgatttaca
    54901 agaaataaat ctgcaattga cttacttcca gacggcagaa gaagtgatca cgagaaaggt
    54961 tgaacatcag acaagagagc agttagaaac tttttttaaa aagctgactg aagattataa
    55021 agaatggctt gtttttcaag aaaactggcg cactgtacgc aatgcctctt tgatggcttt
    55081 gaaattcccc catgatgagt ataggaaagg tcagcgtgaa ctagcggtag ctgcttataa
    55141 aacgatccgt acgaaacaga aattgtttgt agaagcacct accggaacag gaaaaacaat
    55201 ctctacgtta ttccccgctt tgaaagctgt aggggaagaa gagggagaaa agatctttta
    55261 tctaacggct aaaacaataa ctagacaagt agctgaagat gctatgacag cattaaaaga
    55321 tacaggagct gaagtaaaaa gtgtcacttt gacggcgaaa gacaagatct gctttttaga
    55381 tgagacgacc tgtaatccag atcagtgccc atatgccaat ggttattaca atcggattaa
    55441 tgaaggttta tgggatcttt tgaatcatga gaatcagatc actcgtgaag tcatcgaaac
    55501 ttatgcaaga aagcatacac tttgtccttt cgaactatca ttagatgtca gtatctggtg
    55561 tgacgtaatc attggggatt acaattattt atttgatcca actgtttatt taagacgttt
    55621 ttttgaagat gaaaaaaacg aggactatct gtttttgatt gacgaagcac ataatttggt
    55681 gaatcgttcc cgagagatgt attcggcaga actatcttat gaaaaaacga aacgaagtaa
    55741 ggaagctata ccaaaggaat tcaaaaaact tcatcgccgt ttcaacaaac tattaaaaga
    55801 atttgattcg atccgagaaa tagcaaaaga agatcattgg gattatcatc atcaaaaagc
    55861 accggctgaa tctttagtaa aggcaggtta tcaattaagc gaaaaaatta aagaatggct
    55921 ggcagaattt cctgagcacc catcacaaga acaattgctt ccttattatt ttgatttgct
    55981 tcattttttg aaggtcagtg aattttatga tgatcactac gaaacaacag tcgaaaaaac
    56041 gtatcgcgat ctgatagtaa aagagttttg tattgatcca agcttgtttc ttgagcagtc
    56101 gttggataaa ggaaaaagca gtattctttt ttctgctagt ttttcaccac tctcttatta
    56161 tcaggaaaca ctggggggac aaaaaagtct ggcttacaaa ctgccgagtc ctttcccaga
    56221 aggaaaccag caagttttca tcgcaaatta cctagagacg acttatcgaa aaagggaaca
    56281 cagtctggca aaattagtag agacgattca tgcttttgct gatggaaagg tcgggaatta
    56341 tttcgttttc tttccttctt atcaatattt agatcttgcg gtggaagcat tcagacagca
    56401 atatcccgac agcaatgtgc taatccaaga aacggacatg aatgaggaag aacgcgaacg
    56461 atttttaaca aaatttcaaa caaatcccgc tgaaacattg attggatttt gcgtacttgg
    56521 cgggattttt tcagaaggaa tcgatttgag aggggaccga ttgatcggca gtatgatcgt
    56581 aggtgtaggt ttgccgcaga tgaatcatga gcaggaactg atcaaatcat attatgatga
    56641 gaaggaacac cttggatttg cttacgctta tcagcttcct ggaatgaaca aagtcttgca
    56701 agctggtggt cgagtgattc gtggcatggc ggatcaagga atcgttgtat tggcagatca
    56761 acgattttct agtttttctt atcgacgttt attcccacaa cattggcaaa cagcaagaga
    56821 agtacgaagt gttggggaat tggaagaagg aatacagcgt ttttggttat caaaagaagc
    56881 agtaaatcaa aaaaacgagg aatgacagac gtcagcctcg ttttttgatt aacgatagtt
    56941 agtgaattgc aactcgattg gtaagtcaag tttacgtaat aaagcaatca cagcttgtag
    57001 atcatcacgg ttcttacctg tcactctaat ctgatcttct tggatttgtg cttttacttt
    57061 cagtttttca tttttgatag ctgtagtgat tttcttcgcg ttttcacgat cgattccgtt
    57121 gaccaaatcg gcagtttgtc gagctttgcc acctagagcg tgttctgatt cggaaaaatg
    57181 gatatttttg attggcacat ttcttttgat cagcttacca aataatacat ctttgatctg
    57241 ttcgattttg aaatcatcat cgctcaatac aactagattg ctgttctcta atttgatttc
    57301 tactgtagaa tccttgaagt caaagcgatt ggacaattct tttgttgttt gctgaatcgc
    57361 attttttact tcttctgtat tgacttctga tacaatgtcg aagctagcat cttttgccat
    57421 aggaaccctc ctgatataca tataaatatt taccatacat ctatcttatc ataggattag
    57481 ggcagattct tacctgtttg ctaaaattct tctcatttga gttcgaagta ttttatttta
    57541 atagataaac tgttattctt acatttggaa taccaaagta ggggagaggt gcttgtgagt
    57601 aaaattatga ctgcgtatca taaaaatgaa agattaaaga aaatagtggt tattttgata
    57661 accggattga ccgctgcggt cgctttaaat tattttttga ttccagccaa tgtcttttct
    57721 gcagggatga atgggatcgc tcaaatcatt tcctcgcttt tatatgaatg gtttcatatc
    57781 aagatagaca caggaacatt tatttttctt ttgaatatcc ctgtgtttgt attagggttt
    57841 ctcaaagtag gaaaacgagc aacgatcctt agttttgtga atgttgtatg tgtatcggtg
    57901 atgacgatgc tgcttccagt cggacagatc actgataata ttttgatgaa tgccctagta
    57961 ggcggtgttt tattaggtct tggcgtagga ttatctttaa aaatgggctt tacaactggt
    58021 gggatggata tcatctctct tgtactttca caaacaactg gaaaaacagt cggaaactac
    58081 atgctgattc taaatggtat cattgtagtc atcgcaggct tcttctttaa ctgggaaagt
    58141 gccttgtata cgattatttc catttatgca atgactcaag taattgatgc catccacacg
    58201 agtcatcaaa aagtaacagc aatgatcgtt accgtcaagc cagaagaggt aacagaagaa
    58261 ctagcaaaac ggatggtacg tggaatgaca ttactgccgt ctgtcggtgg ctattcaaaa
    58321 aaacaaggga acatgatcat gatggtcatc acccgctacg aaatgtatga tcttgatcag
    58381 atcgttcatg aaatcgacga cgatgcattt atcaatattt taccaactca ttcagtattt
    58441 ggccggtttg caaatgaaaa cgaacaaaaa atgtatcgaa caactggcgt attgccagaa
    58501 ataaaaaatc gttcacaaaa aaaatgagga atcaggttta ttttagaata aatttaatct
    58561 attacatcta ataagaacat ttttataaat ttaaacgaga atcttacttc taaattctca
    58621 tttacaagca gctaaaaaaa gagtatgata aataaaaatc gtgttaagga gtgtcgtacg
    58681 atgagcgaag caaaccaaga acggtctgga caagaaatag aactaatcaa agaacgtatt
    58741 cttgctgcct tggaaatggt gattgatcct gaattaggaa tcgacatcgt caatttaggc
    58801 ttgatttatg atatagaatt caatcctgaa aatggagaaa cagtcattaa gatgactcta
    58861 acaactatgg gctgtccatt agcagatatt ttaacagaat ctatccatga cgcattaaaa
    58921 gaagtgccag aagtaacgaa agcagaagtc aaactagtct ggtatccagc atggacgaca
    58981 gataagatga gccgttatgc acggattgcg ttggggatac gatagaaatc ttggctgact
    59041 agctatgaga agcaaatagg aaaaacaaaa aagcctttta tcctttctag aagaaaagag
    59101 ataaaaggtt tctttgttta taaaattttg tctgctatat tataagttaa ttaaataaat
    59161 gtgttctatt gataaaggac attcaaatcc ctttcctttt ggaatccttc ttttttacat
    59221 cagtttgatt ttcgtatagt attctttctc atcttcacga taaatcgcag ccccattttt
    59281 ttgttgtgct ttaagactgc caagattttt tttatttaca gacaaaagtg cctcatcaat
    59341 gccgattttt tttgcctctt gtagtactaa tgctaatcct tgtgtggcat atcctttttt
    59401 acgataatta ggggagatgc cgtaaccaat atggccagcg ccgtttctta atgtttcgtt
    59461 cagtttatgg cgcaaattaa aaattcctac atactcatgt tcatcacttt ctaaaataaa
    59521 gacggttgcg ggaacgtaac tctcaggaag atcgattcct aaagacgttt tttcgcattt
    59581 ttttacataa tctaaaaatt cagcaaatgt atagccataa gcattgttga taaatccact
    59641 ctcatttggt ccaaagctag tttgtagctg ataagctttc tttaaatctg cttcccatag
    59701 tttgatcagt ttcatcgtat tcacctcttt aagtaacctt attcatttgt tttctactat
    59761 agcactattg ttgtaaatag ccatgcctat ttttatttat tgtgtcatat cgtattaaga
    59821 atcatcgaac aatacaaata tgaattacaa taaaaaaatt atttgaagtt cgttcattcg
    59881 ttacttatga tctctagaag aaagaaaacg tgaattactt cagcgtctag aattccagaa
    59941 ggatagtaat caacagaaga aataatcaat atccaaatga aagaagatag ctgaatcgag
    60001 aaaaagagga aaaaagaaat gaagaaaaaa ataaaatagt tactaatccc agagtcgttc
    60061 gtttaacatt gaaatcgatc atatggataa aacagatttt ttcaccaacc ctcaatagat
    60121 gatggagttg ctcggatgaa ttatttgtgt agatggcata agggagggcg tacagaaatg
    60181 aaaaagaaca aaaatatgtt cttgacaaaa aacacatgta atcctaaact aaaagatgtg
    60241 atgcggctat ggcggaattg gcagacgcgc tagcttcagg cgctagtgag ggtgacctcg
    60301 tggaagttcg agtcttctta gccgcataat ttgttttttt ataaagagaa ggtacacctg
    60361 gagcgatgag aaagaaaata acatattatc taaaagaata taggaaaaat tttaaacaac
    60421 ggaaccagat tcaaataaat caaaaaaaat ttgtgtgaag gtgggacagt ttaaaaccta
    60481 atagattgat tcaaatagta gatttataga ttctgataca agaaatactg gcaaacgata
    60541 gttaaggaat aaagactaaa cttaaactca aaaagaggtc atgcgaaaga ataaatcgca
    60601 tgacctctta ctatattttg aatgtgttta aacgttcaaa taatgaatct tagtaccaac
    60661 cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt
    60721 gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa gaagaatcta
    60781 attggtaacg gccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt
    60841 gtgcgatcca ttcttttgca gagcttgtac ttgctggagt agcagcagtt tctgttacag
    60901 caggagcagg ttgtgtttca gcaacaggcg ctgcttgtgt ttccgtttga accgtttctt
    60961 gtacaacagg ttgttcagct tgaacaggtt cagcttggac tggtgcttct tgaacggctg
    61021 cttgaggttc agcagctgct tgtgcagagc cttctgttgg gattgttaat tgttgtcctg
    61081 agta
//
LOCUS       DAJSNV010000007        60758 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSNV010000007 DAJSNV010000000
VERSION     DAJSNV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.007"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..769)
                     /locus_tag="ON516_000370"
     CDS             complement(<1..769)
                     /locus_tag="ON516_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR3343883.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="ON516_000371"
     CDS             complement(974..1324)
                     /locus_tag="ON516_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343884.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="ON516_000372"
     CDS             1919..2365
                     /locus_tag="ON516_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3343885.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="ON516_000373"
     CDS             2989..3981
                     /locus_tag="ON516_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3343886.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="ON516_000374"
     CDS             3997..4881
                     /locus_tag="ON516_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3343887.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="ON516_000375"
     CDS             4930..6537
                     /locus_tag="ON516_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3343888.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="ON516_000376"
     CDS             6538..8022
                     /locus_tag="ON516_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3343889.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8199..9191
                     /locus_tag="ON516_000377"
     CDS             8199..9191
                     /locus_tag="ON516_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3343890.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9243..10112
                     /locus_tag="ON516_000378"
     CDS             9243..10112
                     /locus_tag="ON516_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3343891.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(10532..12097)
                     /gene="guaA"
                     /locus_tag="ON516_000379"
     CDS             complement(10532..12097)
                     /gene="guaA"
                     /locus_tag="ON516_000379"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3343892.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(12156..13079)
                     /locus_tag="ON516_000380"
     CDS             complement(12156..13079)
                     /locus_tag="ON516_000380"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3343893.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13275..13880
                     /locus_tag="ON516_000381"
     CDS             13275..13880
                     /locus_tag="ON516_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3343894.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            14188..15204
                     /locus_tag="ON516_000382"
     CDS             14188..15204
                     /locus_tag="ON516_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3343895.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            15345..16646
                     /locus_tag="ON516_000383"
     CDS             15345..16646
                     /locus_tag="ON516_000383"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3343896.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            16692..17354
                     /gene="deoC"
                     /locus_tag="ON516_000384"
     CDS             16692..17354
                     /gene="deoC"
                     /locus_tag="ON516_000384"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3343897.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            17375..17773
                     /locus_tag="ON516_000385"
     CDS             17375..17773
                     /locus_tag="ON516_000385"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3343898.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            17839..18921
                     /locus_tag="ON516_000386"
     CDS             17839..18921
                     /locus_tag="ON516_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3343899.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            19133..20695
                     /locus_tag="ON516_000387"
     CDS             19133..20695
                     /locus_tag="ON516_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343900.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            20688..21821
                     /locus_tag="ON516_000388"
     CDS             20688..21821
                     /locus_tag="ON516_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343901.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            21821..22777
                     /locus_tag="ON516_000389"
     CDS             21821..22777
                     /locus_tag="ON516_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343902.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            22876..24042
                     /gene="deoB"
                     /locus_tag="ON516_000390"
     CDS             22876..24042
                     /gene="deoB"
                     /locus_tag="ON516_000390"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3343903.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            24066..24884
                     /locus_tag="ON516_000391"
     CDS             24066..24884
                     /locus_tag="ON516_000391"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3343904.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            24901..25608
                     /gene="deoD"
                     /locus_tag="ON516_000392"
     CDS             24901..25608
                     /gene="deoD"
                     /locus_tag="ON516_000392"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3343905.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(25906..26592)
                     /gene="gpmA"
                     /locus_tag="ON516_000393"
     CDS             complement(25906..26592)
                     /gene="gpmA"
                     /locus_tag="ON516_000393"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3343906.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(26764..27345)
                     /locus_tag="ON516_000394"
     CDS             complement(26764..27345)
                     /locus_tag="ON516_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3343907.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(27478..28161)
                     /gene="rpiA"
                     /locus_tag="ON516_000395"
     CDS             complement(27478..28161)
                     /gene="rpiA"
                     /locus_tag="ON516_000395"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3343908.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            28480..28893
                     /gene="rpsL"
                     /locus_tag="ON516_000396"
     CDS             28480..28893
                     /gene="rpsL"
                     /locus_tag="ON516_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3343909.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            28977..29447
                     /gene="rpsG"
                     /locus_tag="ON516_000397"
     CDS             28977..29447
                     /gene="rpsG"
                     /locus_tag="ON516_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3343910.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            29541..31628
                     /gene="fusA"
                     /locus_tag="ON516_000398"
     CDS             29541..31628
                     /gene="fusA"
                     /locus_tag="ON516_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3343911.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            31800..32987
                     /gene="tuf"
                     /locus_tag="ON516_000399"
     CDS             31800..32987
                     /gene="tuf"
                     /locus_tag="ON516_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3343912.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            33394..33702
                     /gene="rpsJ"
                     /locus_tag="ON516_000400"
     CDS             33394..33702
                     /gene="rpsJ"
                     /locus_tag="ON516_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3343913.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            33733..34362
                     /gene="rplC"
                     /locus_tag="ON516_000401"
     CDS             33733..34362
                     /gene="rplC"
                     /locus_tag="ON516_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3343914.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            34390..35013
                     /gene="rplD"
                     /locus_tag="ON516_000402"
     CDS             34390..35013
                     /gene="rplD"
                     /locus_tag="ON516_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3343915.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            35013..35303
                     /gene="rplW"
                     /locus_tag="ON516_000403"
     CDS             35013..35303
                     /gene="rplW"
                     /locus_tag="ON516_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3343916.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            35342..36175
                     /gene="rplB"
                     /locus_tag="ON516_000404"
     CDS             35342..36175
                     /gene="rplB"
                     /locus_tag="ON516_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3343917.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            36218..36496
                     /gene="rpsS"
                     /locus_tag="ON516_000405"
     CDS             36218..36496
                     /gene="rpsS"
                     /locus_tag="ON516_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3343918.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            36519..36866
                     /gene="rplV"
                     /locus_tag="ON516_000406"
     CDS             36519..36866
                     /gene="rplV"
                     /locus_tag="ON516_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3343919.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            36880..37536
                     /gene="rpsC"
                     /locus_tag="ON516_000407"
     CDS             36880..37536
                     /gene="rpsC"
                     /locus_tag="ON516_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3343920.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            37539..37973
                     /gene="rplP"
                     /locus_tag="ON516_000408"
     CDS             37539..37973
                     /gene="rplP"
                     /locus_tag="ON516_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3343921.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            37963..38151
                     /gene="rpmC"
                     /locus_tag="ON516_000409"
     CDS             37963..38151
                     /gene="rpmC"
                     /locus_tag="ON516_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3343922.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            38176..38442
                     /gene="rpsQ"
                     /locus_tag="ON516_000410"
     CDS             38176..38442
                     /gene="rpsQ"
                     /locus_tag="ON516_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3343923.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            38500..38868
                     /gene="rplN"
                     /locus_tag="ON516_000411"
     CDS             38500..38868
                     /gene="rplN"
                     /locus_tag="ON516_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3343924.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            38905..39213
                     /gene="rplX"
                     /locus_tag="ON516_000412"
     CDS             38905..39213
                     /gene="rplX"
                     /locus_tag="ON516_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3343925.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            39241..39780
                     /gene="rplE"
                     /locus_tag="ON516_000413"
     CDS             39241..39780
                     /gene="rplE"
                     /locus_tag="ON516_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3343926.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            39799..39984
                     /locus_tag="ON516_000414"
     CDS             39799..39984
                     /locus_tag="ON516_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3343927.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            40020..40418
                     /gene="rpsH"
                     /locus_tag="ON516_000415"
     CDS             40020..40418
                     /gene="rpsH"
                     /locus_tag="ON516_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3343928.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            40450..40986
                     /gene="rplF"
                     /locus_tag="ON516_000416"
     CDS             40450..40986
                     /gene="rplF"
                     /locus_tag="ON516_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3343929.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            41147..41503
                     /gene="rplR"
                     /locus_tag="ON516_000417"
     CDS             41147..41503
                     /gene="rplR"
                     /locus_tag="ON516_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3343930.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            41524..42024
                     /gene="rpsE"
                     /locus_tag="ON516_000418"
     CDS             41524..42024
                     /gene="rpsE"
                     /locus_tag="ON516_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3343931.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            42039..42218
                     /gene="rpmD"
                     /locus_tag="ON516_000419"
     CDS             42039..42218
                     /gene="rpmD"
                     /locus_tag="ON516_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3343932.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            42262..42702
                     /gene="rplO"
                     /locus_tag="ON516_000420"
     CDS             42262..42702
                     /gene="rplO"
                     /locus_tag="ON516_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3343933.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            42702..43997
                     /gene="secY"
                     /locus_tag="ON516_000421"
     CDS             42702..43997
                     /gene="secY"
                     /locus_tag="ON516_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3343934.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            44059..44706
                     /locus_tag="ON516_000422"
     CDS             44059..44706
                     /locus_tag="ON516_000422"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3343935.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            44897..45115
                     /gene="infA"
                     /locus_tag="ON516_000423"
     CDS             44897..45115
                     /gene="infA"
                     /locus_tag="ON516_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3343936.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            45148..45264
                     /gene="rpmJ"
                     /locus_tag="ON516_000424"
     CDS             45148..45264
                     /gene="rpmJ"
                     /locus_tag="ON516_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3343937.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            45282..45647
                     /gene="rpsM"
                     /locus_tag="ON516_000425"
     CDS             45282..45647
                     /gene="rpsM"
                     /locus_tag="ON516_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3343938.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            45675..46064
                     /gene="rpsK"
                     /locus_tag="ON516_000426"
     CDS             45675..46064
                     /gene="rpsK"
                     /locus_tag="ON516_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3343939.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            46144..47082
                     /locus_tag="ON516_000427"
     CDS             46144..47082
                     /locus_tag="ON516_000427"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3343940.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            47110..47490
                     /gene="rplQ"
                     /locus_tag="ON516_000428"
     CDS             47110..47490
                     /gene="rplQ"
                     /locus_tag="ON516_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3343941.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            47762..48601
                     /locus_tag="ON516_000429"
     CDS             47762..48601
                     /locus_tag="ON516_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3343942.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            48577..49446
                     /locus_tag="ON516_000430"
     CDS             48577..49446
                     /locus_tag="ON516_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3343943.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            49436..50233
                     /locus_tag="ON516_000431"
     CDS             49436..50233
                     /locus_tag="ON516_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3343944.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            50436..51182
                     /gene="truA"
                     /locus_tag="ON516_000432"
     CDS             50436..51182
                     /gene="truA"
                     /locus_tag="ON516_000432"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3343945.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(51637..54306)
                     /locus_tag="ON516_000433"
     CDS             complement(51637..54306)
                     /locus_tag="ON516_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3343946.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(54544..54732)
                     /locus_tag="ON516_000434"
     CDS             complement(54544..54732)
                     /locus_tag="ON516_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3343947.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            55002..55364
                     /locus_tag="ON516_000435"
     CDS             55002..55364
                     /locus_tag="ON516_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3343948.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            55416..57098
                     /locus_tag="ON516_000436"
     CDS             55416..57098
                     /locus_tag="ON516_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3343949.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            57215..59251
                     /gene="recG"
                     /locus_tag="ON516_000437"
     CDS             57215..59251
                     /gene="recG"
                     /locus_tag="ON516_000437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3343950.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            59300..60301
                     /gene="plsX"
                     /locus_tag="ON516_000438"
     CDS             59300..60301
                     /gene="plsX"
                     /locus_tag="ON516_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3343951.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            60423..60665
                     /gene="acpP"
                     /locus_tag="ON516_000439"
     CDS             60423..60665
                     /gene="acpP"
                     /locus_tag="ON516_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3343952.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        19521 a        10898 c        13194 g        17145 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     8101 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     8161 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     8221 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     8281 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     8341 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     8401 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     8461 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     8521 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     8581 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     8641 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     8701 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     8761 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     8821 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     8881 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     8941 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     9001 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     9061 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca atagaaaaac
     9121 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     9181 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     9241 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     9301 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     9361 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     9421 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     9481 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     9541 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     9601 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     9661 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     9721 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     9781 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     9841 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     9901 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     9961 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
    10021 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
    10081 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
    10141 ataccggaag tttaaaaggg tattctgaaa cttaaatagt ctcaattcat tcttctttct
    10201 tgagaatctg agtagagact tttattctgt ttcaaattaa tataattacg aaatgatatt
    10261 gagtacaaga aagttctttt taccagatct tttaaatcga aaaatctgat atatatattt
    10321 atagtactaa aaatctgatt agataaaaag ctaattcaat aggaaaataa aggtagtgaa
    10381 taaaagcaaa gcccttgtta gtagttaatc taacaagggc tttggtaggg aaaatggtag
    10441 gaaaccatct tattttttac gaaaatcagg aactttagta attgaataat gttgatttat
    10501 aggtattcct catccatatt atgttggctc tttattccca ctccactgtt gctggtggtt
    10561 tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag
    10621 agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg
    10681 aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga
    10741 cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag
    10801 ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag
    10861 tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga
    10921 ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt
    10981 ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag
    11041 atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt
    11101 gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat
    11161 atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact
    11221 tgtctaagaa gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta
    11281 gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag
    11341 atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc
    11401 cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt
    11461 gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa
    11521 agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac
    11581 cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga
    11641 ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt
    11701 gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt
    11761 tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt
    11821 gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc
    11881 caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat
    11941 tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa
    12001 gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa
    12061 tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa
    12121 attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa
    12181 tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag
    12241 atatattcct gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag
    12301 gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc
    12361 gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca
    12421 tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat
    12481 tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg
    12541 tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca
    12601 gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct
    12661 ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc
    12721 gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg
    12781 gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact
    12841 tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata
    12901 taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg
    12961 cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta
    13021 cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata
    13081 ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc
    13141 accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt
    13201 cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga
    13261 gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt
    13321 ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg
    13381 tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg
    13441 aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt
    13501 tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg
    13561 taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat
    13621 cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga
    13681 tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga
    13741 agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga
    13801 aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca
    13861 tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg
    13921 gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga
    13981 actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata
    14041 aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc
    14101 aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg
    14161 gaatttagat agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg
    14221 gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt
    14281 gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat
    14341 ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc
    14401 actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc
    14461 tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa
    14521 gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag
    14581 aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga
    14641 ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct
    14701 gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga
    14761 tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg
    14821 ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca
    14881 tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg
    14941 gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca
    15001 gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg
    15061 cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa
    15121 tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc
    15181 gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag
    15241 gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct
    15301 aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc
    15361 tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca
    15421 cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt
    15481 tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg
    15541 gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg
    15601 gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag
    15661 tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag
    15721 caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa
    15781 gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg
    15841 ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga
    15901 gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg
    15961 ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga
    16021 aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag
    16081 ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag
    16141 aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag
    16201 ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt
    16261 tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc
    16321 cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg
    16381 tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta
    16441 aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg
    16501 acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt
    16561 ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt
    16621 tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa
    16681 ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat
    16741 ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct
    16801 gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg
    16861 tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa
    16921 caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt
    16981 tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa
    17041 aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca
    17101 tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt
    17161 tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag
    17221 aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg
    17281 aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg
    17341 gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat
    17401 caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg
    17461 cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc
    17521 atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga
    17581 aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg
    17641 tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg
    17701 agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga
    17761 aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt
    17821 ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta
    17881 gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc
    17941 aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac
    18001 cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt
    18061 tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca
    18121 aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta
    18181 aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat
    18241 gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct
    18301 tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc
    18361 ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca
    18421 ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct
    18481 gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc
    18541 ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa
    18601 tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa
    18661 tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga
    18721 acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac
    18781 actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa
    18841 gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca
    18901 gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag
    18961 gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg
    19021 ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg
    19081 aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca
    19141 agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa
    19201 tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg
    19261 tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa
    19321 aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg
    19381 gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat
    19441 cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga
    19501 gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga
    19561 tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga
    19621 tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat
    19681 cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt
    19741 agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg
    19801 tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac
    19861 ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga
    19921 aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt
    19981 tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat
    20041 ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga
    20101 catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga
    20161 ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac
    20221 ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca
    20281 tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa
    20341 agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc
    20401 tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat
    20461 tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga
    20521 attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt
    20581 cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta
    20641 taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca
    20701 gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag
    20761 gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct tctatgctag
    20821 atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga
    20881 tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt
    20941 ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc
    21001 tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg
    21061 cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa
    21121 tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg
    21181 aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt
    21241 taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa
    21301 tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc
    21361 gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga
    21421 tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat
    21481 taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa
    21541 tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt
    21601 tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga
    21661 tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt
    21721 tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat
    21781 cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat
    21841 tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg
    21901 cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat
    21961 gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac
    22021 accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc
    22081 agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat
    22141 ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa
    22201 catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg
    22261 accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct
    22321 agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa
    22381 tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact
    22441 gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa
    22501 cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg
    22561 taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt
    22621 gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat
    22681 tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc
    22741 aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg
    22801 ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag
    22861 aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt
    22921 ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt
    22981 gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt
    23041 cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa
    23101 gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa
    23161 actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa
    23221 ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat
    23281 tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc
    23341 cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa
    23401 ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg
    23461 tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat
    23521 ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt
    23581 ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc
    23641 aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc
    23701 tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat
    23761 gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa
    23821 gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat
    23881 catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca
    23941 ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct
    24001 tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa
    24061 agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag
    24121 gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg
    24181 aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag
    24241 tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca
    24301 tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac
    24361 gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca
    24421 acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg
    24481 aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc
    24541 aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg
    24601 atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga
    24661 tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca
    24721 tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag
    24781 caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac
    24841 aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa
    24901 atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga
    24961 gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat
    25021 caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa
    25081 ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat
    25141 ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt
    25201 cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc
    25261 caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata
    25321 gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac
    25381 aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg
    25441 gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg
    25501 accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg
    25561 ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa
    25621 aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg
    25681 acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt
    25741 gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
    25801 gatgaatatt gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa
    25861 gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt
    25921 tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc
    25981 aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga
    26041 gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc
    26101 ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc
    26161 gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc
    26221 cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta
    26281 tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa
    26341 gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca
    26401 tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc
    26461 tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc
    26521 agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac
    26581 taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt
    26641 ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat
    26701 gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag
    26761 gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt
    26821 tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata
    26881 gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc
    26941 ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat
    27001 gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc
    27061 ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa
    27121 ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg
    27181 catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata
    27241 tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa
    27301 atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta
    27361 gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat
    27421 agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta
    27481 tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga
    27541 aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc
    27601 taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc
    27661 tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc
    27721 aaaaatatga agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt
    27781 cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt
    27841 agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc
    27901 actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt
    27961 taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt
    28021 gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt
    28081 tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc
    28141 agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt
    28201 cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag
    28261 ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca
    28321 aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt
    28381 tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg
    28441 tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta
    28501 gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga
    28561 tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca
    28621 actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt
    28681 gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta
    28741 caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt
    28801 taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt
    28861 tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg
    28921 cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc
    28981 cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat
    29041 tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca
    29101 ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt
    29161 ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg
    29221 gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc
    29281 gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa
    29341 aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc
    29401 ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt
    29461 tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa
    29521 agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg
    29581 gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata
    29641 ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg
    29701 aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg
    29761 gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac
    29821 gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac
    29881 ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg
    29941 caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc
    30001 gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag
    30061 gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga
    30121 tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat
    30181 tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag
    30241 aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct
    30301 acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg
    30361 ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa
    30421 ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta
    30481 aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt
    30541 tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta
    30601 tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg
    30661 ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc
    30721 tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta
    30781 aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc
    30841 ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg
    30901 aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg
    30961 taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta
    31021 agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac
    31081 caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc
    31141 gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag
    31201 ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg
    31261 attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga
    31321 aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt
    31381 atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag
    31441 ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt
    31501 atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc
    31561 actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag
    31621 ctgaataatt tcttgaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt
    31681 ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta
    31741 catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa
    31801 tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg
    31861 ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg
    31921 gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa
    31981 tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg
    32041 actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg
    32101 gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc
    32161 tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg
    32221 ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat
    32281 acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag
    32341 gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc
    32401 caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa
    32461 ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg
    32521 acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg
    32581 aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt gctttactac
    32641 gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca
    32701 cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc
    32761 atactccatt cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag
    32821 gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg
    32881 aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag
    32941 gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt
    33001 taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg
    33061 ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa
    33121 gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt
    33181 tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt
    33241 actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt
    33301 gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt
    33361 ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt
    33421 ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca
    33481 aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac
    33541 acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca
    33601 cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag
    33661 ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat
    33721 ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc
    33781 actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta
    33841 caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg
    33901 cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct
    33961 aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa
    34021 attacagtag atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt
    34081 aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt
    34141 tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa
    34201 aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt
    34261 gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa
    34321 aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg
    34381 aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc
    34441 acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc
    34501 gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt
    34561 cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct
    34621 atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac
    34681 agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa
    34741 gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa
    34801 gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa
    34861 aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact
    34921 tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact
    34981 gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc
    35041 cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg
    35101 acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag
    35161 tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag
    35221 gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc
    35281 aattattcga agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa
    35341 cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga
    35401 ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca
    35461 tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg
    35521 ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac
    35581 gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt
    35641 gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc
    35701 agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt
    35761 tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac
    35821 aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga
    35881 agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca
    35941 cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt
    36001 acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc
    36061 tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg
    36121 taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact
    36181 ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg
    36241 accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa
    36301 aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac
    36361 catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg
    36421 acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa
    36481 gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag
    36541 ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc
    36601 ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat
    36661 ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg
    36721 acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga
    36781 aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata
    36841 tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca
    36901 attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag
    36961 tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct
    37021 gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt
    37081 cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga
    37141 aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa
    37201 ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt
    37261 gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg
    37321 atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac
    37381 tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa
    37441 gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt
    37501 ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa
    37561 aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag
    37621 cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg
    37681 aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa
    37741 ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag
    37801 gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag
    37861 gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga
    37921 aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc
    37981 agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt
    38041 aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta
    38101 cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag
    38161 gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg
    38221 gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct
    38281 atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca
    38341 gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt
    38401 ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga gattttccta
    38461 tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt
    38521 ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga
    38581 tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg
    38641 ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca
    38701 ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt
    38761 cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt
    38821 gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact
    38881 tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc
    38941 actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa
    39001 gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct
    39061 caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac
    39121 tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt
    39181 tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga
    39241 atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt
    39301 aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt
    39361 ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc
    39421 actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt
    39481 gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta
    39541 gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa
    39601 gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa
    39661 gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca
    39721 aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa
    39781 ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca
    39841 aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat
    39901 cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc
    39961 ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa
    40021 tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta
    40081 aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga
    40141 aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc
    40201 gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct
    40261 ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt
    40321 taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa
    40381 aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg
    40441 tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt
    40501 actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt
    40561 tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac
    40621 agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta
    40681 ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa
    40741 ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca
    40801 ccagctggag taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac
    40861 aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat
    40921 aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt
    40981 aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa
    41041 gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat
    41101 ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc
    41161 agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac
    41221 tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat
    41281 tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg
    41341 tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga
    41401 aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca
    41461 agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca
    41521 ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac
    41581 cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc
    41641 ggtgacaaaa acggacacgt aggatttggt acaggtaaag ctcaagaagt tcctgaagca
    41701 atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca
    41761 acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct
    41821 gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg
    41881 gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca
    41941 acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa
    42001 tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt
    42061 taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc
    42121 ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca
    42181 atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa
    42241 cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc
    42301 caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc
    42361 ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga
    42421 caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac
    42481 tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct
    42541 gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct
    42601 aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa
    42661 gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag
    42721 atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg
    42781 tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc
    42841 tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact
    42901 tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc
    42961 ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta
    43021 aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg
    43081 gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag
    43141 caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag
    43201 gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta
    43261 tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg
    43321 atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt
    43381 tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata
    43441 cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg
    43501 gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag
    43561 cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact
    43621 ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc actttcttct
    43681 acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt
    43741 atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc
    43801 gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc
    43861 aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta
    43921 tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg
    43981 ttggattcat tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat
    44041 ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga
    44101 acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg
    44161 ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat
    44221 aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa
    44281 ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa
    44341 gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat
    44401 gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga
    44461 gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt
    44521 gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct
    44581 atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac
    44641 actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa
    44701 aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata
    44761 aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag
    44821 aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta
    44881 caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga
    44941 aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac
    45001 tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt
    45061 tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta
    45121 ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc
    45181 gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca
    45241 aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag
    45301 tagatattcc tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta
    45361 acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg
    45421 aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag
    45481 gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc
    45541 gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac
    45601 gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag
    45661 gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata
    45721 tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc atgatcactg
    45781 atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca
    45841 aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg
    45901 aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag
    45961 caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag
    46021 ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt
    46081 tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata
    46141 agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc
    46201 aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt
    46261 cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta
    46321 ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac
    46381 attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt
    46441 accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt
    46501 aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca
    46561 gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt
    46621 ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag
    46681 aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat
    46741 ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg
    46801 gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa
    46861 gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc
    46921 tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct
    46981 gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca
    47041 aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg
    47101 gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg
    47161 cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg
    47221 aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat
    47281 gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat
    47341 gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac
    47401 gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt
    47461 gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg
    47521 attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc
    47581 ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca
    47641 attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat
    47701 tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat
    47761 catggagcca attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg
    47821 ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg
    47881 acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga
    47941 atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg
    48001 acagatggtt ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga
    48061 agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg
    48121 tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg
    48181 tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga
    48241 tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat
    48301 ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga
    48361 tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg
    48421 tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga
    48481 tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag
    48541 tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta
    48601 gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg
    48661 acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc
    48721 ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa
    48781 cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc
    48841 atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct
    48901 ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca
    48961 ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga
    49021 gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta
    49081 ttggatgaac caacggcagg acttgatccc cagggcagaa aagaaatgat ggagatgttt
    49141 tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat
    49201 gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg
    49261 gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct
    49321 actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct
    49381 ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat
    49441 gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc
    49501 ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag caaataattg
    49561 ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga
    49621 tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc
    49681 tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat
    49741 cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt
    49801 tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta
    49861 cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc
    49921 gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca
    49981 gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat
    50041 tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc
    50101 tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg
    50161 gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt
    50221 tttaagagga taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca
    50281 agaaaggatg caagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac
    50341 aggcctcttt cttttatctg aaaagaaaga acgaaaaaat tttatagaaa aatcattatt
    50401 ccagataacc ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg
    50461 cgtacgacgg aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag
    50521 aagaagtaga aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt
    50581 cgggaaggac agatgccgga gtacatgcga tcgggcaagt aatccatttt gattatccag
    50641 aggagcgtcc gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg
    50701 ctgtaagaca agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa
    50761 agacgtatca atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg
    50821 caagttattt tccttaccca cttgatttgg aaaaaattca gcgggcgctt cctgatttat
    50881 tagggactca cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaaatac
    50941 gtacgatcta cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat
    51001 ttaggggaaa tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa
    51061 ttggcaatgg ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta
    51121 atgcagcagg acctacagca caccctgaag gactttattt gtatgaagtg aaatatgaat
    51181 aagaggttat gatagaagtg gtcagcttca agaaataaga aggtatttac gaaaattact
    51241 tctcaaaatt ttgcaaaatt tcggcttatt ttcgaagaag ctacttctgg aacaccgttt
    51301 aaggaagagg tcgtgaaatc agcgttttga cctgagtatt agagaaacaa acgggaaaat
    51361 agctcctcat attttgccgt ttgttgagct aataccgaag gtcagctgat tgaacaccgt
    51421 ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca atttcaaaaa
    51481 acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca
    51541 tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc
    51601 ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc ttcttctgtg
    51661 tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg
    51721 gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa ggattgcacc
    51781 tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg ctggaacttt
    51841 tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat caattttagc
    51901 tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag cggtatttac
    51961 agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt tgatctgatt
    52021 cattgcggct tgataggcta caacatctag accaacgact ttaggctcca tgaaacttat
    52081 ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc
    52141 aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac cattgacagt
    52201 gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc
    52261 ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt tatctgctgt
    52321 ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa
    52381 agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat tgatcaagtt
    52441 tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc
    52501 agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc cgctttgacc
    52561 agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt tatatttgat
    52621 tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca aatttgtccc
    52681 tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc ttatagaaga
    52741 aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata ctggatccag
    52801 attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa gagctttcaa
    52861 gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat caagaatgcc
    52921 atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata ataacaatat
    52981 actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa aagcataaat
    53041 ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt agcttccttc
    53101 aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga ttcctaacaa
    53161 cagcccacga tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca ggagcactaa
    53221 gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca taccatgcca
    53281 gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa gactgccgta
    53341 caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac agaggactaa
    53401 tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt cataaacacc
    53461 aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca acgtagtgat
    53521 ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata caatgatcat
    53581 tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt ttttatattc
    53641 tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc caatcataaa
    53701 acccatataa aaactggata aaaacaagag taaataactg ataaataaaa gtttcggccg
    53761 tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa aaaatgcatc
    53821 cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt aactgacgct
    53881 aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa
    53941 tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca tcatctggtt
    54001 tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa
    54061 ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc caagaagaac
    54121 atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact
    54181 tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa tcaaaaacgg
    54241 aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact ttaaattttt
    54301 tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc atatccttgt
    54361 ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga ataaataact
    54421 tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca
    54481 cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt
    54541 caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt
    54601 aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt
    54661 tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca
    54721 aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct
    54781 cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa
    54841 ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt
    54901 ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag
    54961 aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa
    55021 cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg
    55081 cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta
    55141 ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg
    55201 gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc
    55261 gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt
    55321 cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg
    55381 taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa
    55441 gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg
    55501 tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat
    55561 ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag
    55621 cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt
    55681 ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg
    55741 atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag
    55801 tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa
    55861 aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt
    55921 tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag
    55981 ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag
    56041 cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc
    56101 atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa
    56161 tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc
    56221 gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca
    56281 aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt
    56341 cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg
    56401 atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag
    56461 cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct aattatgtta
    56521 tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag
    56581 taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc
    56641 aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag
    56701 gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga
    56761 tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct
    56821 caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc
    56881 tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg
    56941 aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg
    57001 aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag
    57061 atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc
    57121 taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa
    57181 gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac
    57241 tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac
    57301 gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct
    57361 aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt
    57421 gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga
    57481 tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga
    57541 ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat
    57601 tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg
    57661 tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga
    57721 agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt
    57781 aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt
    57841 ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc
    57901 agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa
    57961 gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct
    58021 tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt
    58081 ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt
    58141 gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa
    58201 tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct
    58261 tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga
    58321 tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt
    58381 gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc
    58441 gactccgatt cctagaacat tagctattac agcatatggt gagatggatg tatctatcat
    58501 tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt
    58561 ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat
    58621 ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga
    58681 aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa
    58741 gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt
    58801 ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat
    58861 ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg
    58921 aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg
    58981 gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt
    59041 acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc
    59101 tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg
    59161 gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat
    59221 agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac
    59281 ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc
    59341 tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt
    59401 actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat
    59461 tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa
    59521 aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt
    59581 attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat
    59641 caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc
    59701 cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca
    59761 atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt
    59821 cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta
    59881 tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct
    59941 actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa
    60001 atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga
    60061 agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc
    60121 agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat
    60181 taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac
    60241 aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata
    60301 gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat
    60361 gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa
    60421 agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga
    60481 tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa
    60541 ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg
    60601 aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta
    60661 aataatttca aataaaagca aagaaggata aaaagctgca tgcagctttt tatccttctt
    60721 tttttaatct ttttgacacg taaaagtcat ttttctga
//
LOCUS       DAJSNV010000008        59283 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSNV010000008 DAJSNV010000000
VERSION     DAJSNV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.008"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            120..1637
                     /locus_tag="ON516_000440"
     CDS             120..1637
                     /locus_tag="ON516_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3343953.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1609..2898
                     /locus_tag="ON516_000441"
     CDS             1609..2898
                     /locus_tag="ON516_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3343954.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3073..3969
                     /locus_tag="ON516_000442"
     CDS             3073..3969
                     /locus_tag="ON516_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3343955.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3983..4846
                     /locus_tag="ON516_000443"
     CDS             3983..4846
                     /locus_tag="ON516_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3343956.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4852..5415
                     /locus_tag="ON516_000444"
     CDS             4852..5415
                     /locus_tag="ON516_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3343957.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5437..6948
                     /locus_tag="ON516_000445"
     CDS             5437..6948
                     /locus_tag="ON516_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3343958.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6960..7919
                     /locus_tag="ON516_000446"
     CDS             6960..7919
                     /locus_tag="ON516_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3343959.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            8005..9084
                     /locus_tag="ON516_000447"
     CDS             8005..9084
                     /locus_tag="ON516_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3343960.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9336..10736)
                     /locus_tag="ON516_000448"
     CDS             complement(9336..10736)
                     /locus_tag="ON516_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR3343961.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10954..12615
                     /locus_tag="ON516_000449"
     CDS             10954..12615
                     /locus_tag="ON516_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR3343962.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12572..13270
                     /locus_tag="ON516_000450"
     CDS             12572..13270
                     /locus_tag="ON516_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3343963.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13286..14710
                     /gene="araA"
                     /locus_tag="ON516_000451"
     CDS             13286..14710
                     /gene="araA"
                     /locus_tag="ON516_000451"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3343964.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            15017..16177
                     /locus_tag="ON516_000452"
     CDS             15017..16177
                     /locus_tag="ON516_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR3343965.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            16295..18154
                     /locus_tag="ON516_000453"
     CDS             16295..18154
                     /locus_tag="ON516_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3343966.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18264..18698
                     /locus_tag="ON516_000454"
     CDS             18264..18698
                     /locus_tag="ON516_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343967.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18957..19118)
                     /locus_tag="ON516_000455"
     CDS             complement(18957..19118)
                     /locus_tag="ON516_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343968.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19137..20159)
                     /locus_tag="ON516_000456"
     CDS             complement(19137..20159)
                     /locus_tag="ON516_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3343969.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20287..20640
                     /locus_tag="ON516_000457"
     CDS             20287..20640
                     /locus_tag="ON516_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3343970.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20652..21818
                     /locus_tag="ON516_000458"
     CDS             20652..21818
                     /locus_tag="ON516_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3343971.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21910..22530)
                     /locus_tag="ON516_000459"
     CDS             complement(21910..22530)
                     /locus_tag="ON516_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3343972.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22722..23018)
                     /locus_tag="ON516_000460"
     CDS             complement(22722..23018)
                     /locus_tag="ON516_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3343973.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23222..23872
                     /locus_tag="ON516_000461"
     CDS             23222..23872
                     /locus_tag="ON516_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3343974.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23888..25615
                     /locus_tag="ON516_000462"
     CDS             23888..25615
                     /locus_tag="ON516_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343975.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25612..27504
                     /locus_tag="ON516_000463"
     CDS             25612..27504
                     /locus_tag="ON516_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343976.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28208..29077
                     /locus_tag="ON516_000464"
     CDS             28208..29077
                     /locus_tag="ON516_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3343977.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29286..30311
                     /locus_tag="ON516_000465"
     CDS             29286..30311
                     /locus_tag="ON516_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3343978.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30350..31300
                     /gene="manA"
                     /locus_tag="ON516_000466"
     CDS             30350..31300
                     /gene="manA"
                     /locus_tag="ON516_000466"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3343979.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31394..33097
                     /locus_tag="ON516_000467"
     CDS             31394..33097
                     /locus_tag="ON516_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3343980.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33099..35132
                     /locus_tag="ON516_000468"
     CDS             33099..35132
                     /locus_tag="ON516_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3343981.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35143..35583
                     /locus_tag="ON516_000469"
     CDS             35143..35583
                     /locus_tag="ON516_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3343982.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35783..36910)
                     /locus_tag="ON516_000470"
     CDS             complement(35783..36910)
                     /locus_tag="ON516_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3343983.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36913..37590)
                     /locus_tag="ON516_000471"
     CDS             complement(36913..37590)
                     /locus_tag="ON516_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3343984.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37854..39620
                     /locus_tag="ON516_000472"
     CDS             37854..39620
                     /locus_tag="ON516_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3343985.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39635..40072
                     /locus_tag="ON516_000473"
     CDS             39635..40072
                     /locus_tag="ON516_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3343986.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40085..41236
                     /locus_tag="ON516_000474"
     CDS             40085..41236
                     /locus_tag="ON516_000474"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3343987.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41298..41996)
                     /locus_tag="ON516_000475"
     CDS             complement(41298..41996)
                     /locus_tag="ON516_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3343988.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41989..43341)
                     /locus_tag="ON516_000476"
     CDS             complement(41989..43341)
                     /locus_tag="ON516_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3343989.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43564..44964)
                     /locus_tag="ON516_000477"
     CDS             complement(43564..44964)
                     /locus_tag="ON516_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3343990.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45125..45862
                     /locus_tag="ON516_000478"
     CDS             45125..45862
                     /locus_tag="ON516_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3343991.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45987..46466
                     /locus_tag="ON516_000479"
     CDS             45987..46466
                     /locus_tag="ON516_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3343992.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46463..47230
                     /locus_tag="ON516_000480"
     CDS             46463..47230
                     /locus_tag="ON516_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343993.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47460..48029
                     /gene="lepB"
                     /locus_tag="ON516_000481"
     CDS             47460..48029
                     /gene="lepB"
                     /locus_tag="ON516_000481"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3343994.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48133..48735)
                     /locus_tag="ON516_000482"
     CDS             complement(48133..48735)
                     /locus_tag="ON516_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3343995.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48841..49310
                     /locus_tag="ON516_000483"
                     /pseudo
     CDS             48841..49310
                     /locus_tag="ON516_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            49416..50000
                     /locus_tag="ON516_000484"
     CDS             49416..50000
                     /locus_tag="ON516_000484"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3343996.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            50003..51076
                     /gene="prfA"
                     /locus_tag="ON516_000485"
     CDS             50003..51076
                     /gene="prfA"
                     /locus_tag="ON516_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3343997.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51069..51908
                     /gene="prmC"
                     /locus_tag="ON516_000486"
     CDS             51069..51908
                     /gene="prmC"
                     /locus_tag="ON516_000486"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3343998.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51901..52080
                     /locus_tag="ON516_000487"
     CDS             51901..52080
                     /locus_tag="ON516_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3343999.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52102..53127
                     /locus_tag="ON516_000488"
     CDS             52102..53127
                     /locus_tag="ON516_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3344000.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53302..54546
                     /locus_tag="ON516_000489"
     CDS             53302..54546
                     /locus_tag="ON516_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3344001.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54699..55646
                     /locus_tag="ON516_000490"
     CDS             54699..55646
                     /locus_tag="ON516_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3344002.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55780..56409
                     /gene="upp"
                     /locus_tag="ON516_000491"
     CDS             55780..56409
                     /gene="upp"
                     /locus_tag="ON516_000491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3344003.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56548..56790)
                     /locus_tag="ON516_000492"
     CDS             complement(56548..56790)
                     /locus_tag="ON516_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344004.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56911..56983
                     /locus_tag="ON516_000493"
     tRNA            56911..56983
                     /locus_tag="ON516_000493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56944..56946,aa:Thr,seq:cgt)
     gene            complement(57102..57740)
                     /locus_tag="ON516_000494"
     CDS             complement(57102..57740)
                     /locus_tag="ON516_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3344005.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(57863..58495)
                     /locus_tag="ON516_000495"
     CDS             complement(57863..58495)
                     /locus_tag="ON516_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3344006.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            58846..59145
                     /locus_tag="ON516_000496"
     CDS             58846..59145
                     /locus_tag="ON516_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3344007.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        19740 a        10318 c        12187 g        17038 t
ORIGIN      
        1 tcattattaa tattttggtt aattaaaatc gataaaaaat aagtaagttt aacatttata
       61 ttattgacaa cgctttcaac gaggtgtatt attcatgtat aaattaaagg aggtcagtta
      121 tggaagccac aatgttgatt agtagtgtca agcgtgtagg caaagttgat ccgcggttgt
      181 acagttcatt tattgaacat atgggacgag cagtatatga agggatttat cagccagatc
      241 atccatcagc taataaaagt ggatttcgcc aagacgtcat tcacttggta aaagaattga
      301 acattcccat gattagatat cctggtggaa attttgtgtc aggatttcgt tgggaagaca
      361 gtattgggcc catagcaaat agacctagaa ggttggattt agcttggcgg acaattgaaa
      421 cgaatgaagt tgggattcat gaattttata actggtgtca gcatgtgggg gccgaaatca
      481 atatggctgt caatctgggt actcgtggag tagatgcagc tcgtaattta gtcgaatatt
      541 gtaattttcc taaaggaact tattggagtg atttaaggcg ttcgaatggt caagagagcc
      601 cttttggaat taagacatgg tgtttaggga atgaaatgga cggtccttgg cagattggac
      661 ataaaacagc ggaagaatat ggacggttag cagaggaaac ggctaaggcg atgaaactcg
      721 tagatgattc aattgaggtg gttttatgcg gaagttcgaa tcggcaaatg cctacatttg
      781 gtgattggga gttaacagtt cttgatcaag cgtacgatca aatagattat ttgtctctgc
      841 atcaatatta tggcaaccaa aaaaatgatc tttctcatta tcttgcacgt tccttagaca
      901 tggatcggtt catttctgaa gtgacagcga tgtgcgatac agtcaaggcg aagaaacata
      961 gtaagaagca gatcaacctg tcctttgatg aatggaatat ttggtatcat tcagcggaac
     1021 aagatgagca agcagcgcct tggcagattg ctcctccttt actagaagat aactataatt
     1081 ttgaagatgc attactatta ggctgtctat taatcacttt attgaaacat gcagatagag
     1141 taaagattgc ttgtcttgct caattggtaa atgttattgc accaatcagg acagaaacaa
     1201 acggagaagc ttggagacag acgatctatt atccatttat gcaagctgct gtgtatggac
     1261 aaggagaggt gttaagccca caaattcgtt cagcaaacta tgcgacggaa gattttgaag
     1321 aagtgccgta tttagaaagt atagccgtaa taaatgacaa agaacttgtc atatttgcag
     1381 ttaaccgtgg agaagaagaa atgaatctaa ctgttcactt gtctgaatta tcattagagg
     1441 gagtgattga tttttctgaa atgagtggat ttgatacaaa agcaactaat cttcctaaaa
     1501 gtgaacaagt aaaaccaagt cattcaacaa atgtaaaagc tgaagatggg aaaatcaatt
     1561 gtttattgaa accgctatct tggaatgtgg tcagatgtcg tttatcagat gaaggaggaa
     1621 caaaaaatga aaggtaaaaa atggttaatt ggctctttgt tggtagctgc aacaggtatt
     1681 ttaggagctt gcggtggcgg aagttcagca gaatctgatt ctgataaaga agtgactttg
     1741 actttttgga atgggtttac tgcatctgat ggtgaaatat tgcaagaaat cgttaatgaa
     1801 tttaataaaa caaatgataa aaacatcaaa attgaaatgg atatcatgac ttgggccaat
     1861 ttgaatgaaa aacttcctac agcgatttct tctaaaaaag cacctgactt tattgcatta
     1921 aattatccag attttgcaca gtatgtagaa aacggagcgg tacaaccatt agacgatttc
     1981 tgggaatatg atggagtaga taaatctgat tttaaagaaa cggctattga attaggtgaa
     2041 gtagaaggta aacagtattt tgtccccatg caggtacaag gcatgtatat gtattggaat
     2101 aatgatttgt ttaaacaagc tggattagat actgaacaac cacctactac ttgggaagaa
     2161 ttgacagaaa tggcaccaaa attaactgat agtagtaaaa atgtatcagg ttttgtcttc
     2221 aataaggatg gaactgcacc gttatataac tggatgttag caaatggcgg taaattagtg
     2281 aacgatgatt atactaagtc tgaatttgcg tctcctgaga accttaaaac gttaaaagcg
     2341 atccaaaaaa tgatccatgt agacaagact ggtccagaat cgatttctgg cgccgaaatg
     2401 gacaatttaa tgaatgctgg tcaactagcc attgaaatca atgggccatg gttaaataac
     2461 ggattgaaag caaacgagat tgattataat gtgactacct taccacaagg gagtgattca
     2521 gacaaaaaaa cagctatttt agatggcgta ggatatgcca ttccggcaag tacagataat
     2581 aataaaaaag aagctgtcta tgagtttttg aaatattgga atacaactga aattggtaaa
     2641 aaatggagta tcgaaaatgg ttttcctgcc tacttggatt cagtagcaaa agatgaagaa
     2701 gtaaaaaatg atcctattgt atctgaatta tcgaaacaaa tggactatgc agagccatat
     2761 ttaccaggtt ttacccaaat ctctgcgatc aataacgata tcatcaatcc actgattgaa
     2821 aaattattag caggagaaga tcctgagaca ctcatgaacg atgcagatga tgccatcaat
     2881 caattactgc aaaaatagta cagaacttta tacaggatag actgttggca agatacagat
     2941 atctgaatga tcgacctagt atttgcagat aggtgttatt gcaaaaatca cagcgattgt
     3001 tcggatatct tattcagatt cgtttaaacc aagattgtat attttatcaa aatgaaagat
     3061 gaggtggact atatgaacgg attcaccaaa tattggaatc gtcctgatcg tgctgcgtat
     3121 ttgtttttaa ctccatcttt tttagtttta ttattattta ctgtcatacc gcttttagga
     3181 acatttgcta ttagttttac agatttgaat attttcttta catcaagaaa ttttgtaagt
     3241 tttgaaaatt tcacacgtat atttagtgat gaacgagcag ttaattcgtt gattcattct
     3301 ttatatttca ccgtactcga aacgccaaca caaattatcg ttggtttatt agcagcagta
     3361 gcattatcta aaaataccag attcaatcgt ttttgtcgtt cgacatttta cattccagtt
     3421 atttgttcat tgacgtcaat tgcgattatt ttttctatgt tgcttgatcc gaatgtaggg
     3481 gcgattcctt acttgttttc acaagtcggc ttgccgatcc ctcaattttt tcgcgatcca
     3541 acccttgcta tgccgacggt agccatcatg actgtttgga agaattttgg tgttactatg
     3601 acgattctct tgactgctat tcagggaatc tctccctctt tgtatgaatc tgctcaaatg
     3661 gatggtgcaa caaatcgaca gcagtttttt aacattacgc tacctcagat cgttccctcc
     3721 ttaggttttt gtattttaac gaatctgatt ggttcgatgc aagtgtttga ccaagtatat
     3781 gttgcaaccg ctggtggccc acaatttaag acagaaacag ctgttcaata tatttacagt
     3841 cgaggattta cagcacctta tgaattgggc tatgcatcag cactgtcttc ggttttgttt
     3901 atcattgttg caactatagc tatctccact aatgtgtaca tgtcacggaa agaaaaacaa
     3961 atgaaatagg agggaaacct gtatggcacc agaaagctcg attacagtta ctgtgaatag
     4021 tcgaaaagca gttagtggta aaaaaatcat tcgaacgatt agtatgctag ttatattgtt
     4081 ggttattgcg tttccttttt tgtggctgat tatttcttct tttaaacacg aaaaagacat
     4141 tatctcattt ccgcctagaa tatttgcaga tagctatacc ctagataact atattaaggt
     4201 ttggacaacg atccctttat tagattacat caaaaataca gtgatttttg ctggagggac
     4261 agtgatcact tctgtattct ttgattctct tgcggggtat gcatttgctc gtatgcgttt
     4321 caaaggaaaa tcagttttgt tctatttcgt tttgctgaca atgatgatcc cattccaagt
     4381 cttcatgatt cctctattta ttgaagtcaa tttgcttggc atgcttgata cttatgcagg
     4441 tttgatcatt cctcgtatga caactgcatt tggtatcttc atgatgcgtt ccttttttat
     4501 tactttacca gattctttgg aagaggcagc acgcattgat ggattaagtg agtttaatat
     4561 ttttttgaaa atcatgttgc cattgagcaa acctactctt ttatcattag ggattttcac
     4621 gctgatgaat agttggaatg acttgcttta tccattgata ttgacaagta gttcaaaaat
     4681 gagaacttta ccagcagggt tagctttatt tacagggcag aacatttctt tttatggacc
     4741 tgtaatggct ggaacggtta tttcaatgct gccattacta gtggtttata tttttgcaca
     4801 gaaatatttt gttcaaggaa cagctatgtc tggaatgaaa gagtgaggaa gatgaaaata
     4861 gttgagacta aactttatgg acagttgatt gtggtttctg attacatctt attaggtatt
     4921 ctctgggtaa tcgtgtgttt gcctgttatt actatcgtgc ctgctacatg tgctgttttt
     4981 tatgtaatgg cgcaatggaa aggtgaggga tcaggaaata tcatttattt tttctttcaa
     5041 ggaataaagc aacattttgg taagaacttg ttccttagtc tgttaacgat cattatttat
     5101 tttcttgtta atcaattatt agaagaacag acaatcgtac tttcaattag tggatatgta
     5161 gttttactta tttattccat gtttctttta tcctggatat atgtgatttc tgaaaacaaa
     5221 gatatcacat ttttacaaat ttttgaacaa agtgcatgga aagtattttt tgattttttg
     5281 agaaatattc tttgttgcgt attgttatta ggatttgtac tgctcatatt tcttttccct
     5341 ccttttattt ttatctttgc tggtggcgta tggaaacttg tatctattat cttgactata
     5401 ggaaatgaaa ggtagggaaa gctgttggac gaagaagtga aagtaaaaat cagagaggat
     5461 tttacggtag gagagatcag ttctcggctg tttggctcat ttgttgagca tatgggaagt
     5521 gtcgtctata cagggattta tgaaccgggc catgaaacag ctgatggtga cgggtttaga
     5581 caagatgtcc tctcttatgt aaaggaatta gaattaggtg tcattcgcta tcctggagga
     5641 aactttacat ctggttatga ttgggtggat actgtaggta ggaaagaaca acgaccaaaa
     5701 aaaattgatt tagcatggcg aggtatagaa ccaaattctt ttggactcca cgaatttttt
     5761 aaatggatca gtcatgtaca agcgaaaccg atattcacgg tgaatttagg aacaaaagga
     5821 attgatgaag caaaaaattt agtagaatat tgtaattttc caaaaggaac cttctggagt
     5881 gatttaagaa taaaaaatgg gcaaaaaaag ccttttggcg ttaaattatg gtgtttaggg
     5941 aatgagctag acggtccatg gcaagtgggg gctaagagtg cttatgaata tggtaggtta
     6001 gcaaatgaag cctcaaaagt catgaagtta gtggatgatt caattgaaac aatcttagtt
     6061 ggaagctcta cgccacgttt accatcgtac cctgaatggg atcgtatcgc attagaagaa
     6121 gcttatgaga atgtagatta tttagctttg cataactata ttgataagta tgatgaagaa
     6181 gacttgacta aacctcctaa aaatgagcgt gatgatacag cgacatacct tgcaaaagct
     6241 tatcgattcg atcgtcaaat tgaagaaatc gttgctactt gtgattatgt aaaaggatat
     6301 ttgagaagtg agaaaacaat gtatctagcg tttgatgaat ggaatgtaca tgcccaaccg
     6361 gaaaaaagac atagggattt tgaagtcggt agtcccatcg attggtgtta ctttactatg
     6421 gaagatacat tattatttgg ttcattaggg ctggctattt tacgacacgc agatcgcgta
     6481 aagattagtt gtcaatcatt gttagtaaac acaatacctt taatactgac agatgctaaa
     6541 ggacaagcat ggaggaatcc aacttattat gtgatgcaac acttatcgaa atatgctaaa
     6601 ggtcaagtat tgaaacaaca aatcatttgc cctgtttatg attgtgaaaa gataaaaaat
     6661 gttccttatg tggacagtgt gatcgtagat aacggcaatc agctgactat tttcttgatc
     6721 aatcgcacaa atgaacaaca aaagataacg attgaacatg atttcaattt gctagatcaa
     6781 ggagagcact acacattaac cagtagcgat ctgttggaca aaaatacacg tgaagaacca
     6841 gaaaaaataa aacctgaaat aaactatttt tcaatagaaa aacatgacga attaaatgta
     6901 gggatagaaa aatactcttg gaatgtaatt agaataaaga aaatgtgagg aaatttgtta
     6961 tgatgaagac actttataat aatcctatcg ttcggcaacg ggcagatcct tgggtatata
     7021 aacatacaga tggttattat tattttacag gatcggttcc aggctaccaa gtgattgaat
     7081 taagaagggc aaagtcactg aatgaattag aacatgctgc aagcctgatt gtatggcagg
     7141 ctcatgaaga aggaccgatg agtgaattga tctgggcacc tgaagtccat tatatcaatg
     7201 gaaaatggta tatttacttt gctgcaagca atcataaaac tatacgtgat gaatctcatc
     7261 atcaccgcat gttcgtactt gaaaatcagc atatagatcc aatgaataca gattggatag
     7321 aaaagggaca aataaaaacg aaaagggaaa ctttcagttt agacggaaca actttctctt
     7381 ctcaaggaaa gctatattat gtgtgggccc aacaagatcc tgaaattact gggaattcta
     7441 atctttatct atccgaaatg gataatcctt ggacgttaaa ggggaagcag atcctccttt
     7501 ctatacctga gtatgaatgg gaaaaacggg gtttttcagt aaatgaaggt ccagcagtcc
     7561 ttactcgcaa tgggaaaata ttcattacgt attctggtag tgcaacagat gaaaactatg
     7621 ccatgggcct gctgtgggct gatgagcaca agaatctttt attaaaagaa tcatggcata
     7681 aattatcgga gccagtattc gtttcttctg aaaaaaataa gcaatttggt cctggtcaca
     7741 actcgtttac ggtttcagaa gacgggtgct ttgatatttt gatctatcat gctcgaccag
     7801 agaagaacca aaaaggggat ccgttagaca atcctaatcg gcacgcgaac gcgcaatgct
     7861 ttacgtggaa tgagcaagga tttccagaat ttggtgaacc tgttccgtat gccttatgag
     7921 cacaagaaac cttgattttt gcttgaataa accttgcaga ctcgttatga tatatgtacg
     7981 tacaaataaa aaggggcaag gcaaatggca aaatatcaag atattgcaga agagataaaa
     8041 caaaagatcc ttcgtgagga atacgaagta aatacaacca ttccctcaga attcaagctg
     8101 caagaaatat atgacgtcag cagacatacg attcggcaag caatcgctgt gttggtaaat
     8161 gagggctatc tgcgtaaaga aaaagggtcg ggcacatatg tagatgacag ctataagaaa
     8221 gaaaaaacac cattgaacag aacaaaaacg atcggtgtca tcaccacata tctctctgat
     8281 tatatttttc cgtcgattat ccgaggaatc gaaaatgaat tacgagaatc tgggtattca
     8341 ttgcttttag ccagtacgaa taacgatcac gaattggaga aagagtgttt gaagaaaatg
     8401 attgcttatg gggtagatgg tttgatcgtt gagcctacga aaagtaatca gtataaccca
     8461 aacttagcac tttatgttgc tttgagagaa caaggcattc cgatggtgat gatcaatgct
     8521 gcttatgaag agttaaatgc ctcttatatt tgtttagatg atgtgaaagt cggttttatg
     8581 gcagcagaat atttgataaa aaaaggacat cagcgtctgt tgttcatcac aaaaatcgac
     8641 gatcttcaag gaaaatacag gatgaaaggt tttatcagcg cgtgtgaaca gtataatgtg
     8701 cgttttttga cggaagatat cattactttc accacggaaa cacgagaaac aatcgctgaa
     8761 aaaacagtac aacggttgaa agaagaacaa gctttagatg gcatcatttg ctataacgat
     8821 caaatagcta gtttaatgct agataagttg acagaaaatg gctatcatat tccagatgac
     8881 ctatcgatcg taggaacaga cgattcttct ttaagccatt tagggaataa aaaattgaca
     8941 acattgacgc atccaaaaga aaagttggga caagatgccg cacggtggat cgtcacaacg
     9001 attgaaaaag gacaggcaca gccaaatatt ctctatgaac ctgtactagt tgaacgggaa
     9061 acagtgaaag aaaacgaaaa ataagagacg ggtttttacc gcctcttatt gctcctacgg
     9121 ttactcgtcg caatgtttgt gagagctttt gtcatcgctc aaggctgaac gtttctttca
     9181 ccacctctcc actaaacggt gttctcctac caactggtca caagtcacaa tattcatcaa
     9241 acatgagaag ctgttttttg aaattgttcc ttgtgactgg aaactaagcg gttccttctt
     9301 atttcttgaa gctgaccact tctgtcacaa cctctttaag aatgatggat agaaatacgt
     9361 ttaggataag tttttttaga tgcactttgt tcgatttcct ctaaagattt cccacgagtt
     9421 tctggtacga tctcttgaat aaataaaacc cctaaaaagc agatgatccc aaaaatgccg
     9481 aaaacgattt cttgagacat agaagcagtc atcacaggaa aaagcaagcc aactaggaaa
     9541 gaaccaatcc aattaaaaga agaggctagg ccagatgctc gcccgcggac agctagtggg
     9601 aatatttcgc ctacgattac ccatgtcagt ggagcccatg tgcaagaata aaatgcgaca
     9661 taaatacata aaaacaagag aatcaacaac gaatttgtat gagcatccag tacagttcct
     9721 aaaacagctg gtaggatgaa agaaagtccc atcacgctac cgcctatttt caataaagtt
     9781 cgccgattga acttgtctgc aataaccaaa aagagcaaag aaccagcaac taaaatgatt
     9841 ccttggatga tcggccacat caatgcatcg ctcgctgcat gcccagtcgc tttttctacg
     9901 atcagcggaa tatagtaaaa aatagcatta gctccttgaa attgctggaa tactgctaca
     9961 ccaataccgg caatgactaa ggaacgatat ttttctaaaa acaaagtatg ccaagaagtt
    10021 ccggttgtct tttcttgttt gacattcagc tggatttgtt cgaattcctg ttcagcttct
    10081 tttggtgtcc gaatatagtt caatactctt tttgcctctt ccaagcggcc agattgcata
    10141 agaaaacgcg gtgactctgg tagagctaaa acaccgaaaa ataaaatcag cgcggggaca
    10201 gcagcgagcc ctaacatcac gcgccaagcc atcgtttcag gcaacccttt tagcaaataa
    10261 tctgcaacat aggaaaggag catgccagaa gcaatcatca cttgattgat ccctgacaat
    10321 cttcccctta gtctagcagg agccatttca gacatataag caggaactag ggcagatgca
    10381 gcgccaacag ctaaacctag aagaatcctt gctgcaatca gaaaataaga accattgtga
    10441 ggggcagatg cagaaaggac agaaccagcc ataaaaataa gtgcagaaag aagaatcatt
    10501 tttcgtcgtc caagcttatc agaaagaaga ccagataaag agccgccaag gatcgcacct
    10561 aacatgaccg aagacgtgat ccatcctatc aaagaagctt tacctgccaa accccaatca
    10621 tgctgtaaaa aaggtaaggc gccggtcata acaccaatat catatccaaa tagaatgccg
    10681 ccaaatgcgc caaaaaaata aataaacgat gaggaaactt ttttttcttt tttcataagt
    10741 attctctcct tctagaattt cgagaaggcg gttgatcaga ttttgtaagc aatttcattt
    10801 tataacttat gtacgtacaa aacaagtgtt ttttattgta ttcttgtaaa taactgcttg
    10861 acatgtacgt acaaaagtat acaataatca ttgtaagcgc tactaaaaag acggtttgat
    10921 caggtttgac gagaaggagg gagttaaatc attatgaagg aagtaaatgc gcaaagtatg
    10981 ttagatgtca tagatggtcg tacatcttta gggattgagt ttggatcaac taggatcaaa
    11041 gctgtattga ttgattcatc ttttactcct attgccgctg gtagttatga gtgggaaaac
    11101 aaactagaaa atggtatctg gacctattca ttagaagata tttggaaagg actgcgcgtc
    11161 agttaccgaa aaatggcagc agaagtattc gaaatctatg gcgagaatct tacgacaatc
    11221 gggtcgattg ggttcagtgc aatgatgcac ggttatctag catttgatag caaggaacag
    11281 ttactagtac catttcgtac ttggcgaaat acaatcactg aagaagcaga acgaatatta
    11341 acggaaaaat ttcaatacaa tattccgcag agatggagta tcgctcattt gtatcaatct
    11401 attttgaata aagaagaaca tgtgaaagaa attaattttt tgacaacgct tgcaggttat
    11461 gtccattggc agcttactgg tgagaaagta ctaggcgtag gagatgcatc aggcatgttt
    11521 cctatcgatc cttcaacgaa aacctaccat caagaaatga aaaatatttt tggtcagtta
    11581 atcgaaaaag cgcacgtagc gattgacttg gatcacattt tgcctaaagt tctagtagca
    11641 ggagaagaag ctggcaagtt aagcgaatca ggggcttatc tgatcgaccc tacgggaaat
    11701 ttgagaccag gaatcccggt ttgtccgcca gaaggggatg caggaaccgg tatggtcgct
    11761 acgaacagtg tagccaaagg gacagggaac atttcggctg gtacttccgc cttcgctatg
    11821 atcgtattag aaaaagaact agaaaatgtc catccggaaa tcgacatggt cactacacct
    11881 tcaggagaac tagtaggcat ggttcatacg aataactgct cttcggatat caatgcttgg
    11941 gtcagtattt ttgaagaatt taccaattct ttgggtatgg agataagcag agataaatta
    12001 ttcacggtat tattcaacaa agcgctagaa ggcgatacgg attgcggggg attactttct
    12061 tatggttatt tttccggtga aaacattaca ggagtaaatg aaggaaggcc attatttgta
    12121 cgtacaccag gtagccgttt tgatttagcg aatttcatgc ggatccattt agccagtgct
    12181 tttggtgcaa tgagaatcgg aatggatata ttgaaatctg agaatgtcca aattgatcgt
    12241 ttagtggggc atggggggat ttttaaaaca ccagaagttg gacagcggat tctagcatct
    12301 gctatggagg caccagttac agtgatggac acagccggag aaggtggagc gtggggaatc
    12361 gcgttattag cagcctatat gatggatcga catgggaagt ctttagaaaa cttcttggca
    12421 gaagacgtgt ttggacaaga ccaaggaatc accatcacac cttcagcaga agaaatagca
    12481 ggctatcaga tatttatgag acgttaccgt gaggggatcg acattgaaaa aacagcgatt
    12541 catcaattgc aagaaaaagg agtgatagaa aatgctggaa caactgaaag aagaagtata
    12601 ccaagcaaat cttgacttgc ctaaacacgg actcgtcaaa tatacatggg gaaatgtgag
    12661 cgcttttgat cctgaaacac gatatttcgt tatcaaacct agcggagtca gttatgaaga
    12721 gatgacagca gacgacatgg tcgttgtgga cttggacaat catatcattg aaggtaaact
    12781 caatccctct tcggatacgc ctactcatgc tgttttatat cgttcatttc ctcaaatcgg
    12841 aggaatcgtc catactcact cgacatgggc gacgatctgg gcacaagcag gattggatgt
    12901 tcctgcgatg gggacgacgc atgcagatac tttttatgga tcggtacctt gtgctcgatt
    12961 tttgacacaa caggaaatcg acagcgggta cgaatatgaa acaggaaaag ttatcataga
    13021 aacattcaaa gaacgcaaaa ttgatccctt agcaattcca ggggtacttt tacacggaca
    13081 cggtccattt acttggggga aagatgcgca aagtgcggta atgaacgcag tcgtactaga
    13141 tgaagtatgc aagatgaatt tatttaccag acagctcaat tcattttcag aagagctgcc
    13201 tcaacggatt ttagataaac attatttacg aaaacacgga aaaaatgcgt attacggaca
    13261 aaacaaataa aaggagtggc taattatgtt gaatattgga gaaaaagaat tttggtttgt
    13321 tgtaggatca cagcatttat atggggaaga agcactgcga gaagtaaaga aacatgcaca
    13381 agaaatggtc gatgaactga atgaaaatgg ccaattgcct tatccaatcc gccttcaaga
    13441 attagcagtt actgctgata cgatcacaaa aatcatgaaa gaggtcaatt atcgagaaga
    13501 agtagcaggt gtgatcactt ggatgcatac tttctcacca gcaaaaatgt ggatccgcgg
    13561 gacaaagctt ctacaaaaac cattgcttca tttagctacg caatataacg aaagcattcc
    13621 ttggaaaacg atcgatatgg atttcatgaa tttaaatcaa tccgctcatg gtgaccgcga
    13681 atatgggttc atcaatgctc gattaaataa acaaaataaa atcgtcgttg gctactggaa
    13741 acgacctgag atccaaaaag agattgctga ttggatggat gttgcagtag cctataatga
    13801 aagttttgga ataaaagtgg ctcgttttgg tgacaacatg agaaacgtgg gtgtcacaga
    13861 aggagacaaa gtggaagcac agattcaatt tggttggact gttgattatt ttggtatcgg
    13921 agatcttgta caagtaatcg atcgcgtatc agacgaagaa gtcgatcaat tatttgaaga
    13981 gtacaaggaa ttgtatacct tcgactatgg tgattatgaa gaaaagacat gggaggaaca
    14041 tgtaaaagta caagctcagc aggaaatcgg tattcgccgt ttcttagaag aaggaggcta
    14101 taacgctttt acgactaatt ttgaagacct gtatggaatg aaacaactac ctggactagc
    14161 tgtacaacgt ttgatggcag aaggatacgg ttttgctggg gaaggcgact ggaaaactgc
    14221 agcgattgat cggttgctga aaatcatggc tcgagggaaa gatacaggat tcatggaaga
    14281 ttatacttac gagctagcta gtggccaaga agcaatcttg gaatcccata tgatggaagt
    14341 agatccgacg cttgctgcga cgaaaccaag aatcgttgtt tctcctttat caatgggaga
    14401 tagagaagat ccggcgcgtc ttgtatttga tgggaaagca ggagaagggg tagtcgtatc
    14461 gatggcagat ttcggtacgc attataaact gctgatcaat gaagtagaag cctttgaacc
    14521 aacgactgaa gcacctaatt tacctgttgc acgtgtgtta tggaaaacta aaccgaattt
    14581 ccatgaaggt gtccattcat ggattcaggc aggtggcggt catcataccg ttgtctcgct
    14641 aaatctaaca acagatcaaa ttgaaacgtg ggcaaaatta gttgagttgg aaacagtggt
    14701 tattagataa aaagaggttg tgaaaaagcc acttagtttt aagaaataag aagagattga
    14761 cgaaatttgc ttttcaactt ttttgaaatt tagtcttatt atcaatgtca gctaacggaa
    14821 caccatttat caagatatct caccctcaat attttgctct cgaaaataag gcttatggca
    14881 gtaacttgtc atagccttat ttttatcact cagcctactc tgctctaact gctctttttg
    14941 cttctaggaa gtcaaatggt aaacagttgt ctaattgcag aaatttcgag atacttgtta
    15001 gtaaggaggg ggttttatgt atcacgttca tttgattgat gctattccag aacaagagat
    15061 agaaaagtta caaggggaat atttcaaaat aatagatgat ttgccagccg aaggaatagt
    15121 tattcgtagt acgaaagtga aagatgaatg gcttacacca gatttgctag caatttcaag
    15181 agctggtgtt ggggtgaaca cgatcaacgt tgaaaaagct tctgaaaacg ggacgatcgt
    15241 catgaataca ccaggtgtaa atgctaatgc agtaaaagag cttgttttgt gctgtttgct
    15301 cttatcttcc cgtccgataa tcgaagcaag tcgtatggta caaacactaa caggtcctaa
    15361 tattttagaa caggcagaaa acaaaagaag tgcttatgtg ggacgagaat tgcaaggcaa
    15421 aaccatcggt cttcttggtc ttggagcgat aggcacaaaa gtagcgctaa gttgctatag
    15481 cttagggatg gatgtcttgg gttattctat ccgcgatgcc cagttggatt atgttcggca
    15541 agcagattta gaaacagttc tttctacctc ggattacatc gtagtcatgc tgccgttgac
    15601 agaagataca aaaggattga tcgatcaggc gaatatagaa aaaatgaaaa aagatgctgt
    15661 acttatcaat gtaggaagaa gtgaaattgt tgataaatat gctgttatgc aagcattgga
    15721 aaaacaacat ctggctaaat acttgactga ttttccagaa gaagagttct tagaaaatga
    15781 ccgtatactt atgttgcctc atctaggggg atcgactcag gaagctttgg cagacagcgg
    15841 acgtttggca gttgaagcct taaaagatta tcttttgttc ggtacggtta gagaagcggt
    15901 caattatcca tccgcacgaa tgtttttcca agcgccgttt cgattcacta tattttatca
    15961 aaagaaaacc aatatattag cagagatatt ttctctgctg aatgagaacg agctagcgat
    16021 cgcagatata tcgcgtaacc ataagaatga gcatgtctac atattgattg acatagacag
    16081 tactgatttt gaacagctca atcaagtgaa acaacagcta gaaaaaattt ctggagtacg
    16141 aaaggtccgc ttattaaaga aaccagaaag gccataaaga aagagaatat ttggatttca
    16201 ttaattcttc tttccgttct ttactttcga atttcgtaca actaaaataa gagaagcaac
    16261 attcttttat tgatagaaat gaaggagcaa gcatttgaaa agatatataa gcagactttc
    16321 gaccaaacag aaaaagattt taaaaggaac agcagcaatc atcagtatct tttttcttgt
    16381 tgtttttagt aatttatttt tgcagtggtg tcaaaatgat ctatccgtag accttgcgtt
    16441 gaaatttgct ttttcttggc atacggaaaa attcttctta gcctgtctcg tcttgctgat
    16501 tattcttatt tttctgattg cactagcagg atctgtccct ttaggttccc ttacgtatgt
    16561 cgtagccatc ggtgtattag gttttgcaaa ttatatgaaa atgagttatc ggcaagaacc
    16621 gatttatcca gacgatttga agatgatcac tgaaatcgga ttgctgaaag atatgaccgg
    16681 cactatgctg tttacagtga tcttagctgc cgcaggaacc gtgataggat tgttttgctg
    16741 gtacatgttt cgaagcctga aaaaaggaag gcgattccaa ttgatccgat taacgacact
    16801 gcttgtggca attggactgc tagggtatat cagtaatttc aacaatcccg ataatttgtt
    16861 gcgaaaagca tataataaaa cggcactatg gataccttac agccagaaaa tgaactacta
    16921 taacactggt tttatcggtg ggtttttgta caatctgaag gtagaaccaa tggatgaacc
    16981 tgaaggatat tcgaaagaaa agatcaaaga aatcactgaa aaataccaga agctagccga
    17041 tgaaaaaaat aaaactgtag aggaagagtc gccgaacatc gtgttcgtga tgagtgaaag
    17101 tttctcagat catagtcgat taaacggcgt agaagtatct ggtgagccat tggcagatta
    17161 ttatgaagta gctgatcaaa catatagtgg caatatgctt tctcaaaact atggcggtgg
    17221 aacagcaaat atcgaatttg aagcattgac tggtttttct atggcgttat ttaatgcgca
    17281 actcaccacc ccttacacga tgcttgtacc aaaaatggat cagcttcctt ctatcgtttc
    17341 agcgttaaac gcacaatcat accagacgac agcgatccat ccatacaata cttcgatgta
    17401 taaaagagag gatgtttatc agacattagg atttgaccag ttcatcagcg agcgtacgat
    17461 gacctatacg gatacgatag agaataatcc atatatttct gatgagtcag cttacaaaga
    17521 agtaatgaca cttctcaagg aagaaaaaac acctcagttc attcatttag tcacgatgca
    17581 gacacatatg ccctacgatg gaaagtatga taatctagac tatcctgcaa aaacttcaga
    17641 tggaagtggg acacttgact tagagaatta tctgcaagat atttcctata gcagcacggc
    17701 actgaagcag ttcacggaag aattgaagaa tctttctaga aggactttag ttgttttttg
    17761 gggggatcat cttccaggga tctattcaga tacgatccaa gcaaaaaatg acaaacaaac
    17821 attgcatgaa acacaatttc ttatgttcga cagtaaagga gaattagaaa agcaaacgac
    17881 tcacgatgca atcaccagtc cgttttattt tgcagcgaat ctaatggaac agacgaatca
    17941 gacaacaaat ggcttttatc agctgctttt atcattagaa caggaacttc cggcgtttga
    18001 acgagaactg tactatcaaa acggacagtg gtataaagaa gcgcaattca atcgctcgca
    18061 acaagagatt tacgacgaat atcaattgat ccaatatgac attgtcgcag ggaaacaata
    18121 tagtttagca gatggtttct ttgagcacga ataagaatgg cgagctgtat agaaaccatt
    18181 aaattttttc aagtacttgt catttgagga taaaaacaag tagaataaat aataacaaca
    18241 atggagaaag taggaaaacg aacatgttac cgaatattgg ctattatgtg cagtccatcg
    18301 atcaaatcgt caaggaaaca gaagcaatcg gagaaggaat gcatccgaaa tatgaagtga
    18361 tcagaaacgc aattgatgaa ggaaaaacaa gtgaactgga caaatcgact cttgaagaga
    18421 tcatcctgtt atttgaagaa ggaacaaaaa aatatcgggc aatgcttatg aaagtcagta
    18481 ctttgcgtcc accagcaaaa gcaatgggca ttcataaaaa ttttgaaaaa gcttatcttt
    18541 catatgtagc aggctgtgaa gaaatgatcc aaagtatcga tcttgaatca ggggtagatg
    18601 aagatctatt caatacatct gaagcaaaac aagaccaagc gacagaccaa ttgacagctg
    18661 ccatccaaaa aatgactaat ttgttgttga aaaaataaaa gaaatctttg ataacaataa
    18721 aaactagagt tatctaatat aaatgtaata ttacctttat gatagagtga tcaaaatcct
    18781 ttatgataat gacataccta taaccaacaa caaccttgta cttactcaaa atcgagtaaa
    18841 tactcctttt ccgtcgagtc taaccatctc ggcggttttt tatttgtaga aaagaacatc
    18901 aggatagacg gaaaagtctt gtcctgatgt tcttttcaga tagtatggaa agctgattag
    18961 ttttctacat tctgtacata ttgttcaaag tagttctgta aatcggcttt gagtttctga
    19021 tattcctctt cgctatactc tttatcttcg attttcccat caaagaaagg aatatttagt
    19081 tcttcccgta gtctgtctaa tacgtcttgc tgattcattt cattctcctt ttcttactaa
    19141 cgatcaaaat aaacacagac acattctggt acatagacat cttttttcag taattccagt
    19201 aatcctgttt caggatgacg gcgatataat gtcagattat cggtgttctg attggcacac
    19261 acgacaaaat tattgcttgg atctagtgcg aaatcgcggg gaaaatcccc ttctgttgag
    19321 atgatttgct tgttttgaat gtgggaacca tcttcaaaaa ctgcgaatac agcaatcgag
    19381 ttatgtcctc ggttagaagc ataaaggaac ttgccatcat ttgaaatgcg gatagctgcc
    19441 cctccatttt caccgtcgaa ttcttctggc aaggtcgata ttttctgtaa ttcttcaaaa
    19501 gcacctgttt ctttattata agaaagcacg cggactgtgc tgtctaattc accaaataaa
    19561 taagcgaatt gtccatttgg atgaaagaca agatggcgag ggcctgttcc tggctctgca
    19621 gtgaaggtag ttacaagatt caatttgccg ttttcggaca catcataagt atacacacga
    19681 tctgtaccaa gatcacatac gactaatcgt tggtcaggag tcagatccgt atagtggaca
    19741 tgcgcatgat cttggttttt atgaggacct gtcgcttctt gatggaatat tgaatctgct
    19801 gctttcagtt cgccatctcc taatatatgg tagacattta cttcaccttt atgatagttc
    19861 gccccgtata gtaattgacg tgcttcatca accgctacat aacacaaagg agcgccagac
    19921 tctgttaccg cattcaaaaa atgaaaatcc ttatcgtacg caccggcacc acctaatcca
    19981 tccacactag taactgtgta aagattgcca gctttacttt ttgctacata ggtagggcta
    20041 ttttcttcaa cagctaatga aaggttggct aattcttctt tttctgtatc aaggtcgatc
    20101 gtatagattc ctttactttc gcgacgcgta taggttccta aaattatctt ttcgatcata
    20161 tgagctgatt cctcctaatt gtaatgaaat agttttcgtt tttattctat cacataatta
    20221 ttccacacgg ctaaagaatg agaatgtgtt atactgtttt tgaaagcaat taaaggagag
    20281 agtgcaatga ttcaggcaaa gattacagaa attgggacag aagcaataaa caaggaagaa
    20341 cccatgctga ttttgttcga taaaacagca acagctgcct tacgaaaata ttcggtgatt
    20401 caagaagtat caaaagacga acctttttct ttgaaagcag gcggaacaat tgcttttgat
    20461 gatcaggtat atacgatcga ttatgtcggt ccgatggcca atgggaattt aaagacagta
    20521 gctcacgtat cattgatttt tgatgcttgt ccagaagaag gacaaattgc aaatggaatc
    20581 tatcttcatc cgcatagact gcctgctatt ggtttgggaa gccaaatttt ttataactaa
    20641 caggaggaat tatggaagga aaaaataaga agaacagacc ggtatcgtgg ttttggcgct
    20701 ggtttttaaa taatcaagta gtcacagcct tacttgttgt attgttaata ttgctgatcg
    20761 tctttttatt tacaaaagtt tcatatttgt ttgcaccaat ctggcaattt ttagcaatcg
    20821 taggtctgcc tatcatttta gcaggaatct tgtattactt gatgaatccg gtagttgatt
    20881 actttgaaaa gcgaaaagta ccaaggcttt acagtatcat tgggctgttt gttttggttg
    20941 ttgcactaat cgcatggggg agcgtagtga tcatccctaa gattcaggaa caaacagtga
    21001 gcttcatcag taacttccct caatatgtag atacgattga taataaatta accgagattt
    21061 tgcgggaccc gctgttcaac caatttcgcg aacagttgga aacagcaggt gataagttca
    21121 tgaattcggc aggcgatatg atccaagata tatctaagag tacagttcag agtcttggaa
    21181 gttttgtcgg cgcggtagcg actattttag tggctctgct gacgatgccg tttatcttat
    21241 tttatctatt gaaagacggt aaacaattag caccttattt tgtcaaattt ttaccgacac
    21301 gcatgcaaaa accgacgttg aatgtgctga gtgaaatgaa tagtcaagtt tcttcttata
    21361 taagaggaca actaactgtg gcttttgctg tggcaatcat gtttatgatt ggttttgcgg
    21421 tcattggttt agagtatgct gtcacgttgg ggattattgc gggtttttta aacctgatcc
    21481 cttacttagg atcattttta gcgatgattc cggctatctt tttagccatt gtagcaggtc
    21541 cattcatgat catcaaagta ctgatcgttt ttgcagtaga gcagacaata gaaggacgct
    21601 tgatctctcc attagtacta gggaatcagt tagcaatcca tccagtaacg attttattag
    21661 ttctgctgac ttctgggaaa ttgttcggaa tcatcggtgt gatcttgggg attcctgttt
    21721 atgcagcagc taaagtagtg attactcatg tttttgaatg gtacacaaca atctcttctc
    21781 tttatgaaga aaatccgttg aaaaagcaga aagaataaca aaaagagaca gatgggaaaa
    21841 tatccaaggc acaaatgctg ctttgaagat tttttcctct gtctcttttg tatgcattta
    21901 tctcaatttt taaaatgatt tacctaaagc agcagcttta tctaatgcat tttgcatcaa
    21961 ctcatctgca cggtcagggt cataatcgat tccttcgatg aatagctgat ctacttgatc
    22021 gatgcctaca aagtttaaaa taccttttac atattgagag gcaaagtctt gtccgttgta
    22081 tacaccgcca ttagattgga tgtgcagtgc ttttttacct gtcactaatc cttctggccc
    22141 gttttctgta tagcggaacg tttttccagc tacattgacg gtatcgaacc aagcttttaa
    22201 ttttgttggg atatttagat tccataaggc gtttgcgata acgattttat cagcagcaag
    22261 gaactgatca gtcaattcat tgaaacgagc aattttttgt tgttgacttt ctgataagga
    22321 agtgaattct gcaccagcac gtaatgcttc ccaacctgat aataattctt catcgatttc
    22381 cggaatatca tcgcgataaa gttccaaaac agtcacttca tcatctggat gattttcttt
    22441 ataacttgtt aaaaaagacg tcaatgcctt aactgtgcgc gattcttctg cagttagggg
    22501 atgacctttg actactaaaa cagatgccat atgcttacct tctcctttta ttgctatgat
    22561 tgttataact atgactaaca aaatcagttt actcgaaaaa gaaaaggcta tcaagtcata
    22621 ttgataaggt tgaagaataa ataactaaaa aaagacaggg tataaaagag gctgtgacac
    22681 gattttttgt cacagcctct tttgaatcta acaagcaata ttcaagcaac actcgactga
    22741 agagcttcca tcccaccaat gatataaaca gcacgaaaac cattagcagt aagttgatcg
    22801 gctaatgctt ctgatcgcct gcctgtatag cttagaatat aataaagttt ctttggatct
    22861 agatttttta attgtcttgg caagatagca gcggggatag aaagcgagtg gcttaagtgg
    22921 ttttttgtgt aatctaaggt atttcttgta tctaaaacga tgctgtcagc atcgagaggc
    22981 aatttattga agtcttggac actaattgat tgggccattt atctcatcct ttgtttagta
    23041 tgtggctatt ttaatgggca actctcatta attcaaatgg aaggattcct cttatcactt
    23101 ttttaatgta tcggatgata atgaaaaaaa ctatgcaaat gtcttgcaaa acaggtagga
    23161 ggtgttataa tgacatagcg tcatatgaca atgtgtcatc ttgaaagaaa ggagaatcca
    23221 tatgccgaca cagacatttt tccatttgcc taaagaaaaa caaaaaagac taatcgaggc
    23281 tgcgagaatc gagttttcta gagtaccctt aaaagaagct tcgattgcta atatcgtcaa
    23341 attagcggat atccctagag gcagtttcta tcagtatttt gaagataaag aggatcttta
    23401 tttttattac tttgagacaa tgagacgtga cagctctaaa gatatgttac aactaatgaa
    23461 agaatccgat ggtgatctat tcaaaggatt cgaatcctat ttttctaaaa tgatttatga
    23521 gattttggaa ggcgagaatg cttcttttta tcgtaacttg tttatgaaca tggattaccg
    23581 tgcctcaaga aaagtcgctc ctcatacgag tcgccaaaaa acgcctcacg atccacataa
    23641 agaacacaaa caagatgcaa aagagttgct tgaattgatc gatcggacga agctgaaagt
    23701 aaaaaatgat catgaattgg aattactgat ccaattagtg atgaatactg tatttacaac
    23761 aattgcgcat tcgtatcgct tattttcaac atctgaagat tactcagtag agcaagcaat
    23821 cagtgaattc aatttgaaac tttcttggct gaaaaacgga gtatataaat agaaagaagg
    23881 gaactagatg ctaaaaatcg taaaacggat ctccttgact tcagcaattg cagctgcagt
    23941 tttcatggtg atccaagtac tggcagattt atatttgccg acacttacct ccaatattat
    24001 tgataaaggg gtagcgcaag gagatgtcga ttatatttgg catacaggtt ttgtgatgat
    24061 cggcttttct ttgatcagta tcttagctgc tatcgcaaat acattttttg ctactcgtga
    24121 atcacagaaa ctgggtaagc aattgcgtac ggatgtctat aaaaaatcag agagtctgac
    24181 aaaagatgcg tttgataaat atggaacggc ctctttgatc acaagaacga ccaatgatgt
    24241 gactcaaatc caaatggtta cacaaatgtt tttacgtatg atgatcaatg caccaatcac
    24301 gttgattggc gcgagcatac ttgcttatca aaaagatcat cagttgacaa aaatcttctt
    24361 agttgtcatc ccagtaatga ttattttgat cggcggaatc atgtactttg ctgttccatt
    24421 attcaaaagt atgcaaaaga aaactgaccg tttaaacctg gtatttcgag aaggattgac
    24481 aggggtcaga gtgattcgag cattcgataa gacacgcttt gaagaaaatc gttttgattt
    24541 agcgaataaa gattatacaa atacggcaat caaagttaat acgatcgtcg cattgatgat
    24601 gccaatgatg acattgatca tgagcggtac aaacgtagca atcacttggt ttggtggaca
    24661 ctacatagca gatatgactt tggaagtcgg taatctgatt gcatttatga cttatgcgat
    24721 gcagatcttg atcagcttta tgatgctttc cgctattttt atcatggtgc ctcgtgcaca
    24781 agcttcagct gaccgtatta atgaagtgtt ggatgaaaaa atcggtatcc atgaccctga
    24841 aaatcctaaa acagtttctt ttgctggaaa aaacgcaaca ttggccttca atcatgtcaa
    24901 ttatcgatat catggagcag aaaaacttgc attggaagat attgatttcc aagccaagtc
    24961 gggcgagatc gtcgcaatca taggggggac tggttcaggt aaaacgacct tagtcaattt
    25021 gatccctcga ttatatgaca tcgaatctgg ttcgatccaa ataaatggaa cggacattag
    25081 tgacatgact caatataatt tgcgagaatt aatgggcttt gttcctcaaa aagcagttct
    25141 gttctctgga acgatccgag acaatatgca gtatggtaag ccggatgcaa cagatgaaat
    25201 gatttggaaa gcattagaaa tcgcacaagc taaagatttt gtttcagaga tggaagatgg
    25261 cttagatagc catgtggaac aaggaggagg aaacttctct ggtggacaaa gacagcgtct
    25321 ggcaattgca cgggcattag tcaaaacagc agatatctat gtgtttgatg attccttctc
    25381 agcgttggat ttcaaaacgg atgccaatct tcgccaagca ttgaaaacga atatgaaaga
    25441 aagtatcacc gttttagttg ctcaacgagt aagtacagta atggatgcag atatgatcct
    25501 tgtgctagat gaaggaaaac tggttggcaa aggaacacat gaagaactat tagcaacaaa
    25561 cgaaacttat caagaaatcg ttcattcaca actaagagag gaggacttag catgagtaaa
    25621 acaaacacac gtccctctca tggaccagca ggcggtccag gacatggtgg acctggcaga
    25681 aatctcggtg taaaaggtga aaagcctaaa aatttctggg gaacggtacg ccgattgttt
    25741 ggctatatgt ctaaacgtat ggttgcgatc atcgctgtat ttgtactagc aatagcagcc
    25801 gtgatcttcc aaatccaaac accaaaggtc ttggggaaag caaccacaga aatattcaaa
    25861 ggtgtaatgg ctggcgctca acagatgcaa caaggccaac aagtaacaaa atttccgata
    25921 gactttgata aaatcgcgca gatcattgcg acagtaatcg tgatgtattt gatttctgct
    25981 gtattcaact ttttacagca atttattatg acacgtgttt cccaacgaac agtttatgaa
    26041 ttacgtcatg atttggaagc caaaatgaat cgtgtaccga tttcctatta tgatacacat
    26101 acaaatgggg atatcatgtc tcgagcaatc aatgatatgg ataacatcgc aagcacgctg
    26161 cagcaaaatt tgactcaatt tgtaactagt gcagtcacat tgattggggt catcgtgatg
    26221 atgctttcaa tcagctggca attgacgtta gtcgcgttat tgatgatccc tttaagcttg
    26281 atcatcgtga tgatcgtagc gccgaaatca caagtgtttt tcgcagatca gcaaaaaagt
    26341 ttaggtttat tgaataacca agtagaagaa acatatggtg gtcataccat tgttaaaaca
    26401 ttcaatcata ctgaaaaaga ccaggaagta tttgaaaaag aaaatcagaa tctgtatgcc
    26461 gctggttgga aagctcaatt tatttcggca atcatcatgc cgttgatgaa tttcgttaaa
    26521 aatctaggct atgtgttcgt cgctgtacta ggcggagtaa aagttgcaaa tgggaacatg
    26581 acattagggg atgttcaagc gttcttgcaa tatacaacac agttctctca accaatcact
    26641 caacttgcaa gtttgatgaa cactatccaa tcaacagttg cttcagccga acgggtattt
    26701 gaagtgttgg atgaagaaga gatgtccaat gaaaaatctg gccttccaga agaaaaagat
    26761 acaccataca aagttcgttt tgaaaatgtt caattcggct acacaccaga taacttgcta
    26821 atgactgatt ttaacttgga tgtcaaacct ggtgagatgg tcgctattgt aggtccgact
    26881 ggtgcaggga agacgacact gatcaacctt cttgaacggt tctacgatgt ttctggaggt
    26941 agtattcgtt acgatggtgt agatacccgt gatctttcac gtgatgaatt gcgcgcacag
    27001 ttttcaatgg tactgcaaga tacttggctg tttacaggaa gtatctatga caatatcaaa
    27061 tatgggaacg acgatgcaac agacgaacaa atcattgagg ctgctaaagc tgctcacgta
    27121 gacgattttg ttcgtaaatt gccagaagga tataacactg tattaaacga ggatgcaagc
    27181 aatatttcgc aagggcagcg tcagctaatc acaattgcgc gggcattctt agcaaatcca
    27241 gatgtcctga tccttgatga agcaacatct agtgtagata cacgaacaga aatactgatt
    27301 caaaaagcga tgaaccgcct attggaaaac cggacaagtt tcgttgttgc tcaccgcttg
    27361 tctacaatac gcgatgcaga caacattatt gtgatgaatc atggttcgat cgtagaaaca
    27421 ggaaaccacg atacattgat ggaaaaagac ggattctatg cagatcttta taacagtcag
    27481 ttttctgaag aagaagcttc atagaatagt acaaatttat tatttttttt aggggctgtg
    27541 acaaaagtcg tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgtccttcg
    27601 gtattagctc aaaaaacggc aaaatatgag aagctatttt tccgttttgt atctctaata
    27661 ctcaggtcaa accgctgatt ttacaacctc ttccttaaac ggtgttctcc tgccaactcc
    27721 tcggtcacaa gtcacaatag tcaacaaacc tgagaagctg ttttttgaaa ttgctccttg
    27781 tgactcggag ttaaacggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
    27841 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
    27901 tactactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
    27961 gcatctgcgg taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg
    28021 ttcttattac ttgatgcaga acagcttttt cacaacctct ttttataaga gtttttactc
    28081 attaatttac aaaaaacaag caaaatgcga attttctcac atttttttta acaaaatcaa
    28141 agttattgct tgaaagaaga agaatccatc gttaaaataa agcttgacgt agaggggagc
    28201 aagaaatatg aaagattttc aattgggggc tgccacctat ttaaaaagtg tagccattcg
    28261 ggtgaaagac cgagataaaa tgattgattt ttacaagaac gttgttggct ttgatttgaa
    28321 aagagaagaa aacgaattag ccattttagg aacagaagaa gctggaagtg aaatgttgct
    28381 gctggaagaa agtcctagag cgaatgatca catgggggtg atcaaaaagc tgaaccgttt
    28441 ttcattgatt atccctagca cagaagaaat ggcagatatc ctttgtcgta tccgtaaaat
    28501 gaattatcca atcaaagaag ctttagaaga tcatggacgt atggggattt tgctcgtaga
    28561 cccagaagag aatgaattgg aaatttacca tgaagagaat aagaacgacc aaagagaaat
    28621 tccagaaaca atggatcaag aagcattatt aagaaaatct gaagaaaaat tcagtaaact
    28681 atcaacaggt tcttattttg aaaaaatcca tttaaatgtt actaacttaa caaaagaacg
    28741 ccaatttctt aatcatgtac ttggtttgaa ggtacataat gaagaagagg gcatccatgt
    28801 attgaatgaa ggagatttcc atgttggttt gacagaagct agcggaggcg caattgattt
    28861 gcctacagat gaagtgattg gacttgattt tctgaaattc atgatcgata ctgaaagcat
    28921 ggaaggactg attgctcact tgcagcaact atcagttgat ttcttcttag acaaaaagca
    28981 cacagtactc acaatctatg atgctatagg aatcgaatgg tggttcgtca gtgagccaaa
    29041 agaaaagaat aaaactgaca ttcgatgcga cgcttaactt tttctttctt aacgattgtc
    29101 gcatgtctcg tcattttatg aacggtcata ctatcgctta ttctagagat agaaagagaa
    29161 aatagtatac cgggggaaac tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt
    29221 gattcctcat agattaagct ttaagcttgg actgtaagaa tctgatagaa aagcaggaga
    29281 aaaagatgta tccacacacg aaacagctta ttttgaaaaa gatggaagaa ggattttatc
    29341 caggtgtagc agtcgccttc ataaaagaaa atcaagtaga aacatttaca gccggctttg
    29401 cccaagtcgt tcctgaaaag cagctgatga cagaagatct gttgtttgat gcagcctcac
    29461 tgacgaaagt cgttgcaacc acttcattag ttttacgtct attagaagat ggaaagatag
    29521 aactcgatca accgcttcat aaatatcttc ctgaatttga aaatcagaca atcactttgc
    29581 gtcatttact gacacatact tctgatatca aaacatggat accaaacaga gataagctat
    29641 ctcaggaaga attgatcgcc gcttatttga agctagaacc aggtgagcaa ctaggaaaag
    29701 aagtaaaata tacagatgcc ggtacgattc ttcttgggtt gatgctagag gaaatttacc
    29761 aagaaactgc agtggaaatc tttcaagaac aagtattgga accattagga atgatgaaca
    29821 gcttatttct tccttttcct tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg
    29881 aggttttgcg tggacaaact catgatccga aagcaagagt tttggctgaa catgcaggta
    29941 acgcgggtct ctttattgat ttgaaagaca gcattacttt tgtccaaatg tatttgaaca
    30001 agggaaaaat ccgtaatggt cgttttttag aagaagagac gattgattct ttactgcaag
    30061 atcaaacacc gaaaaaaaat ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag
    30121 aagacggtca tgcgattctt tttcatactg gttatacagg tacgtttttc ttgattgata
    30181 taaaaacaca agaagcgttg atatttttgt caaatcgcgt acatcctgtc gatcatcgtg
    30241 cggagtatct catatgtcga gatgaaattg ttgaaaactt tttgaaagag aaagcggtat
    30301 taccgaaata atgctataat agctatgaaa aatgagagga gtttgaatta tgcaggaacc
    30361 tatgttttta aaaccagttt ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt
    30421 ctttggtttt gatatcccta acgataaaat cggagaagat tgggcgatca gtgcgcatcc
    30481 tcatggtgtc agtgtagtag aaaatggcga attcaaaggg aaaagattag atgaactatg
    30541 gtcagaacac aaggaattgt ttggccatcc ttcagaacca gtgtttccat tattgatcaa
    30601 gatcttagac gcagaagatg atctatctgt acaagtacat ccagatgatg cctatgggat
    30661 gaaacatgaa ggtgaattag gaaaaactga atgctggtat atcatcgatg ctgaacctgg
    30721 tgcagaaatc atctatggtc atcatgcaaa gacaagagaa gaactagctg agatgatcaa
    30781 agatgggcgc tgggatgacc ttttgaagaa agtaccagtt aaaaaaggtg atttctttta
    30841 tgtaccaagc ggaacgatcc atgctattgg taaaggaatc atgattttag aaacacagca
    30901 aagttctgac acgacttacc gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac
    30961 aagagagctg catatccaac aatcgatcga tgtaacaact gtccctgcga aaacaccaga
    31021 attacaaatc aaagaagtcc gaaaagggaa ttcatcgatc gtcacttatt tagaaactga
    31081 gtttttcaat gtatatgagt gggatattaa aggaatcaca tctttcaaaa aacaagcccc
    31141 ttatacattg atgacggtaa tcgaaggagc aggagacctt gtcgttgacg gaaaaacata
    31201 tccattagaa aaaggaacaa gctgtattat tccaaatggc gtttctgagt gggcggtcca
    31261 aggtgaacta gcaatcattg cgtctgaacc aggcaaatag ttcccagata gctgaaagca
    31321 gcagacggtt tttcgtttgc tgctttttct acataaaatc cgtccatcaa taaatagaag
    31381 tctggtgaaa aagatggaat acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa
    31441 acaactgaga aaagctggac atcacttaaa tgaaatgatc atgccaaatc taagtctgtt
    31501 tatcgcttgg ggaatcttgt cattagcaac aaaaggtgtg tctggcgatt tgcacaccac
    31561 attagaagag gtatgccatt ggatgatcca gctgctttta cccatcttga tcagttattc
    31621 aggcgggaaa tcggtagggg gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg
    31681 gttgatcgct cgttccaatg ctccacaaat cgtaggagcg atgattatcg ggccgcttgc
    31741 cggcatcgta tatgaacaat ttgtttctag attccatgtg aaattcaagg ctggatatga
    31801 aatgcttgtc agcaacctac tagtaggact gactggtagc gttatttgta taattggaat
    31861 cgtttttatt gaaccagttt taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg
    31921 gctagtcaca atgaatctgt taccacttgt cagtctgatc ttagaacctc taaaagtact
    31981 atttttcaat aatgcaatca atcatggtgt attgacacct ttaggaatcg aattctcaca
    32041 aacagtcggg tactcttcct tatttttgat ggaggcaaat cctggacctg gtttagggat
    32101 cttactttct atcctttgtt ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt
    32161 gatgatccat gcgattggcg ggattcacga aatctatttt ccatttgtct tgctgcgacc
    32221 gtatctattt ttagctgtaa tggctggtgg ggcaagcggc acgttgatat ttcaatggtt
    32281 agatacggga ttagctgcac ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc
    32341 gccttatcca caactagctg gggtaagtat agggatagca atcagtacat ttgtgacctt
    32401 catttgtgca gcgatactat tgaaattacc aaataaagaa aaagagaaca aaacggtcaa
    32461 agaaagggga gaaatcttgg aaccatctat cattcattcc gtcatttttg cttgcgactc
    32521 aggtatggga tcaagtgcta tgggagccgc cctgctgaaa aagaaattag cagcagaaaa
    32581 aatcgatctt tctgtcggat acagctctat ctatgaattg aataaccaag taaatcagct
    32641 ggttatcgtg caacatgaac tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat
    32701 ctatgtactg gatcactttt tggaaacaga tatcttgctg tcacgaataa aagaagcgat
    32761 ccaatttcca aagcgggaag aaaaaaaaga gatagcagat atcacagaac aaaaaacgtc
    32821 gcctgctaat attgtatttt tgtataaaaa caacagacgc ggctcacaaa ctatgggcat
    32881 gacgatttta agtcaattag caaaaaaagc gaaaaaacag tttgttatct caaaagaatc
    32941 aatagaaaaa atcaaaatcc aagaagaaac gatttatatc gcacaaagag aactagctgt
    33001 aactgaacag ctagaagaaa agatccctcg gctagttcta gttgaacatt gggtagctac
    33061 gaatgaatac gaaaaactcg tgaagggaga aatataagat gtatttatcg cccagagaaa
    33121 aacaattatt agcagaattt ctcagtagcc cgtcacctgt atctatccaa aaaatgatga
    33181 atttattgaa ggtcagtaaa cggactattt accgcgaatt agataatctt gaagtctcat
    33241 tgaaatcggt aaatgcttct ttgaaaaaag cagctagagg ctcatttgtc attgaggcat
    33301 cagaagaagt attagagcaa ttaaaagaaa atacagatgg gaaaagtaat gacttatcca
    33361 ctgctaaacg acaacacgcg attttagccg agttgttact gacgacagag ccgttgagtc
    33421 ttgctcattt catggaatca tatcgcatca gtaataccac tttttatgca gatatcaaac
    33481 agttggaaga aagtattcgc caactgcctt tggaaatcat tcggaaccag ggatacgaga
    33541 tcgaaggacc ggaaaaatac cgccggcttc tgacagcgaa tgtattagaa ttagaaatca
    33601 atgaatatga actttttcat agtatttctt tcgatgcatc actgaattat ttttttcgat
    33661 tcgttgatcc gcaacatcta tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa
    33721 aaaagaccaa tctaagtgat agaaagcttg aacatttggt tttgatgctg acattgacga
    33781 tggagcgtgt gacgaaaaat catttactga caaatgagac atataatagt ctggcaaaca
    33841 aagaattact acagacagct aaacgattgt tttcacggat cgcttctgag acaaaacaac
    33901 tttatccagt aaatgagatc gtcttttttg caggattatt aaatgacttc acgaactcat
    33961 ttgatcagaa tttctttgaa gagacgttcg atacacagct tgcttattta gtaaaacaac
    34021 tgattcaaga agtaagtgaa gagacgaatg tccattttta tgaagatgaa actttgtaca
    34081 aaatgctgtt gactcattta tctggtgtgt tttcaagagc cgttttacaa gaagagacac
    34141 tgaccaatcc gattttagaa aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc
    34201 gtaaagcagt agagcggatt tttcaagaaa aaaaattatc cgaagaagag atcgcttata
    34261 tggtccttca ttttgcgaat tcattggaac gaagtccgaa agaaagcaat atcagtattg
    34321 caggattttc gccaagtgga ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt
    34381 attttccctt catccaacaa attcatttct atcggattgc tgatttaaaa aaaatcgatt
    34441 tacaagaaga atatgatgtt gttatttcta catctatttt accaggatac ggtggaaaat
    34501 atattttagt ctccccgcta ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt
    34561 cgcatattga gaagaaaaaa aggcaaaaaa ttacgtataa cagcaataac gagtggatga
    34621 tagaagatac ttatgaggaa acaatgcatt tcatggatca aatcaatcaa ttactcggtc
    34681 gtttttttat ccaagaactg gataatcctg agacgatttc cgaaacgatt cgcagcttat
    34741 ttccttattt tcctgaagga ctagtaacgg atgaagaggt ggttcatcgc cggctgctta
    34801 agcgatatga acaagcacct atcggtattc ctcatacaaa tatggggtta ttccatacat
    34861 ccagctcatt gatttctaaa ccgatctttt gtattttcaa tttgaaacat ccgttgacca
    34921 ttgaagggat ggatagacag ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt
    34981 cgccaatcca agaaaaagac gggattcttt taggaaagat cagtggagca atcatcatga
    35041 atgacttgaa caccgagatc ttccgctcag gaaataaaga aatcgtttat cagttgttag
    35101 cgaaaatcct gattgaagaa ataaagaaat gaaggtgaaa agatgataca gctaccagca
    35161 gaaaaaatat tgctcgatca atcctatcaa aattggaaag aagcgttgac cgagatcggc
    35221 cagtggatga cccaacaagg gggaatcaaa ccggcatata tccaatcaat gatcgaaaga
    35281 caagaaaaag cgagtgtcta tatcgggaat ttcgtagcct tgccacatgg gactgacaaa
    35341 gatgaagaag tagtagaaga aggaatctat ttagtccaag tgcctgatgg tgtggacttt
    35401 ggaacaaaag aggaaccaaa aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa
    35461 catcagttag cggtgctcca agatttagcc tttttctgtt cagacatcga ccaagtaatg
    35521 gcgctcagtg atgcggaaac cattgaagaa gtccaaatga tttttgaaca tggacatata
    35581 taaccaatgt tcacagtaaa aaaagctgcc ttgcatcaag taataacttc aaccaagata
    35641 aaaataagag gagctatttt tgttgggagg ttcttattac tgcggatgca agcttttgaa
    35701 aactatttat tctagaaaac cgtttatttg aaatagggga cggtgacaag gtttttgtca
    35761 ccgcccctat tatttttgtg ttttaatagc tcttttttat atagatagtg cccataccta
    35821 tgaataataa aacgatactc catataccaa gtgtcaagat accattcatg aaagtaacct
    35881 gttctgaagc tagtggcgag aaaatatctc ctcctatgat cacattttga aaatcaacat
    35941 aagaagttgg caagtaatga ctgattgatt ctatccatcc aatcgaagaa aacaagcgat
    36001 actctgctaa gaaaagacaa agaagtgtac ctaaaagatt gactagagaa tgttcaaata
    36061 aagcagatag aagaaaactg attgtactta aaaagagcac ccacaacaac aggaacagaa
    36121 agctctggaa aagatactca tacatcggca ggattcgtat ctcctgacca atgatcgttc
    36181 ctacaggata acggaagtct cccaaaccat ttttgattat tactagtata gagacgatca
    36241 aaaatggcat cacgagactt agcatagctg caccataata aatggtaaaa cggatgctat
    36301 gtttctttac catactatcc ggtaatagat tgactagaga gattacctgc tttctttttt
    36361 cataagagat gatggaagaa agaaacaagg cggttataca taaaatcaaa aaactagaaa
    36421 tcattcctga aaaaatattt tcgtagtaat ttgctaaaga gagatgcgct ggcaagtcaa
    36481 aaatagaata tctttggatc tgatgttctt tcatataacg gagcaactcg acacgttttt
    36541 gttgttcaat gattggaatt ccttgtcttt gaccagaaat cagtcgatcc agtatatctt
    36601 tttcataatg ataagttgct tcgaccattt gctggacatt ccctgtgttt tttgcatgta
    36661 agattgcttc gatcagtttg ttgcttttct ctaaactttc cacaaattct ttctcattct
    36721 catcttcttt caacgtttca atcaaacgtt gcatctcttc atagttatga ttcagttcat
    36781 cgaattctgt ataagcaagt ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca
    36841 acaatagagg aaataaaaag tttactttgt tttttagtaa gagattgcgt tcaaatgtaa
    36901 gtaaagacat agttacacct catataattt acgataaata gctaaaatct cttccatatt
    36961 tgaggattct gtgacgattt ctccattttt tagaaaacat gctttagaac aaatcgattg
    37021 gatattcgcg atagcgtggg aagaaagaag aatcgtcttc ccctctttct gccattgtct
    37081 gatgaggcta ttcaagatat caatacttcc tgggtctaat ccgttcaacg gttcatcgaa
    37141 taacatgaca gatgaatcac tgatcaagta agcagcaagc aagacatgtt gtttcattcc
    37201 tagagacatt tttccaatcg gcaaagtaag gtactcagtc atcttcaatc gttggaaaac
    37261 agtttggcat tctactttgc tctgccaata gtatttgatc aaagtcaaat aatcaacagc
    37321 cgatagatag tcggataact gattcgttga ttctagaaaa aagaaagacc ggcgataatc
    37381 tgtataagca gtaggagaga tgttgtgaag ggacaattga tcataagttg ttggcagcag
    37441 ttgggcaatg ccattcagca aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc
    37501 tacttctcct gattctacta aaagattcaa ttgtcgaatc acacgttttt taccacgttt
    37561 catgttcatg tttttgatca gtaatgtcat agataggcct ccaatataaa atttaataac
    37621 ataatagaat ggaaaacatc taaattaaat gctaacaaaa gttattgttt gcatattata
    37681 aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt ctgataccga aatcttttta
    37741 ttttgctgac atactcaaac ctttggcaca aagtttcgtg ccaaagtacg actatttctc
    37801 tttttagtaa gcggatacaa ttagagtatc aaaaggagag agggagagac aaaatggaac
    37861 ggacgattcc caaaaacaac atttcgctaa aagcgagggt acaaaaatta ggcagtactt
    37921 tatcaagcat ggtcatgcca aatattggag cactgattgc ctggggtgta ttaacggctc
    37981 tatttatccc agatggttat ttaccaaatg aggctttcgc taccatggta agcccaatgc
    38041 taacttatct gattccttta ttgatcggtt atactggcgg aaaagtaatt gctggtgacc
    38101 gcggatcagt tgttggggca attgcaacga tgggtgtgat tgtcggaaca gatattccaa
    38161 tgatgctagg cgcgatgatc atgggacctc ttggtggata tgccatcaag aaatttgatc
    38221 agctattcca aaaacggatc aaatccggtt ttgaaatgct agtcaataat ttttcagcag
    38281 gtcttatcgg ttttggatta gcgttattag gtttttctgc gattggtcca gttgtcgatg
    38341 cattgactca agcaatggca aaaggtgtag agatcatttt atctgcccat ttgattccat
    38401 tgacaagtat ttttattgaa ccagcaaaaa tcttattttt gaataatgca atcaatcatg
    38461 ggattttgac accattggga actgaacaag tagcagaggc tggaaaatct attctattct
    38521 tgcttgaagc aaatcctggt ccaggacttg gcgtattact tgcttttact ttgtttggca
    38581 aaggagcagc aaaatcttca gcaccaggag ccatgatcat tcacttttta ggcgggatcc
    38641 atgaaatcta ttttccttac gttatgatga aaccgttatt attcttatca gttataggcg
    38701 gaggaatgtc aggaagtttc attttccaat tgatggatgc tggactaaga gcgccagctt
    38761 ctccagggtc gatcattgcg attctagcga tgacaccgtt caattcttac cttcctgtta
    38821 ttttgggcgt gattgttgcc gcagctgttt cgtttggtat ttctgcagta attttgaaag
    38881 cggatgcgaa agatacaaat gaagacttcg aaaaatctgt tcaagagaca aaggcagcta
    38941 agcaagcttc caaaggtgcc caaggtattc ctcaaggagc tacattctct ggcgtacaaa
    39001 aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga
    39061 gaaaaaaagt acaggaagca gacttgcctc aaacggtgac caatcaagca atcagccaac
    39121 tgacagatga ctcgaataca ttgatcgtta ctcaggtcga actacaagaa agagcaaaac
    39181 aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt tttgaattca cctcgatatg
    39241 atgaaatcat tcaagaatta agcagcaagc agaaaacatc agtttctgaa attgagaatg
    39301 agccagcaga agagtttctt tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc
    39361 acgatgatcg ttccggatca gcaacaatgg ggatgaccgt gctcaaagat atattagaca
    39421 agaaccattt aactatcccg ttaagaaaag tcaatatcca agatttaaaa gatgaacctg
    39481 attcactgat cgtagccaaa gaaagtttag cggaaaaagt acagatggct gcacctcaat
    39541 ccattcattt atcagtcaac agcatggtca atccgcaaaa atacgaatcg attgcacaat
    39601 atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa agtttgccat tcgacatgat
    39661 cgagctcaat cgatctttta aaaccaaaga agaagcgatc cgctattgcg gtcagatgct
    39721 tgtagaagca gggtgcgttg atgaggaata tatcgatgcc atggtagata gagacaaaat
    39781 gttatctgtt tatatgggga attttatcgc gattcctcat ggaacagatg aagcaaaaaa
    39841 atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca gatggtgtag attttggtac
    39901 cgaagaagaa gaaaagattg cgactgtttt gttcggtatt gcaggagtcg gagatgagca
    39961 tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat atggacaatg ttgtccagct
    40021 agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta gcaattgctt aaggagagag
    40081 aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt cgaggattta ttggagaggt
    40141 tttggcagct aatggatttg agattacttt cgtagatgtg aatcacacga tcattgatgc
    40201 tttaaatgaa agaaaagaat acgaaatcga attagcagat gaatcaaaag aaaaaatcac
    40261 tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat
    40321 cgctgaagca gatttagtta ctaccgccat tggcccgaat atcttacctt ttattgctga
    40381 gttgattgca aaaggtatcc aagaaagaga aaaggaagga agcacaacat ctttagatat
    40441 cattgcctgt gaaaatatga ttggcggttc agcatttcta gaaaaagaag tgtataaata
    40501 tcttccggaa acatcattta ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg
    40561 aatcgtccca ttacaacacc atgaagatcc tttattcgtc caagtggaac ctttcaaaga
    40621 atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc caattaaaag gggttttata
    40681 tgtagatgat ctagaaccat atatcgaacg caaactattt agtgtcaata ctggtcatgc
    40741 gactgtcgct tatacaggtg ccttgttagg ttaccagacc attgatgaag caatgcaaga
    40801 tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa acaggaagtt tgctgattgc
    40861 caaatggggg tttgatgcag agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt
    40921 ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct cggacaccat tacgtaagtt
    40981 aggttatcaa gaaagattta cacgtccagt gagagaacta caagaacata acttgacttg
    41041 cccacatttg acggcaacaa tggggattat ttttaattac tatgatcctg aagatgaaca
    41101 aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg gaacaattga ttcaagaagt
    41161 cacaggaatc aatgatccga aaacaatcgg gaatatcaag caaaacgtga accgttatgc
    41221 caagcaagtc gcttaaaata ggatacgaaa aaggggaatg tgacgaaatt tttgtcacat
    41281 tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc caattcttta
    41341 ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc atttcttgca
    41401 aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc ttcagattgg
    41461 tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc accttcacct
    41521 aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa ttcttcgtta
    41581 tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta gtgatccagt
    41641 gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc tagcaattgg
    41701 tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat caaagaatct
    41761 ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc accgccaaag
    41821 aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat ctccgtatgg
    41881 caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc gttatcacca
    41941 tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt agccacggtt
    42001 cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca atacagcagt
    42061 gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt cgattacttc
    42121 tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt ttagacgtaa
    42181 agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca tatcggcaaa
    42241 gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt aattggcatt
    42301 taataaagcc actaaagaaa atggataagg agctagacca atcatatcga ttacttgatt
    42361 gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga tattgatgac
    42421 ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt tatcaggatc
    42481 gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat tatacatacc
    42541 gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca cattgtccat
    42601 ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac aatttggaca
    42661 gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt gatgacaatt
    42721 tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt cttcatggtc
    42781 aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg gtgaatcacc
    42841 gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca ttttataggt
    42901 cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa ataagaacag
    42961 ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt ctaggacagc
    43021 aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa ttccctgtac
    43081 catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca aaatattgac
    43141 tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga cttggtagtc
    43201 ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac ctggtaaact
    43261 tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa ctgcaatcgc
    43321 aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac gaataaattc
    43381 aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc ttaagaaaag
    43441 gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa aaaacagctt
    43501 gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac ctatattccc
    43561 attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc gtgtttcttt
    43621 tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca tcagtgtagc
    43681 aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata aaccagttgt
    43741 tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt ctgtagcaga
    43801 taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa cgactgattt
    43861 tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt cacttttgag
    43921 gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc cgccgcctac
    43981 agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg ttgtgatttg
    44041 aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg aataaaacat
    44101 cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct tttccaatag
    44161 ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag ctaatttggt
    44221 aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata gccattcccg
    44281 gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca tgatcaggat
    44341 caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt caaacccaga
    44401 gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac gccaacgatc
    44461 aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta attgaaacca
    44521 gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac gcggttgatt
    44581 catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag aaaaaatgac
    44641 aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata tttccagcag
    44701 tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc cagtcacact
    44761 cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat ctggttcgcg
    44821 aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat aataaaaaac
    44881 gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc gctgcagcct
    44941 tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt ttctaccagt
    45001 atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat acagctttcg
    45061 attaaaatag aaatggctta taataaggca aatcctagaa aaaagaagga ggaggaataa
    45121 caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa taaattatga
    45181 tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc ctaagatatt
    45241 aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt ctcaatatgc
    45301 agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt atttgcgaag
    45361 attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc atcatgttgc
    45421 gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa caaaccatct
    45481 tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc gattagatct
    45541 tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc tgaccatctc
    45601 gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa agatttacgg
    45661 gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct ctttgcaaat
    45721 gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg ctgccaagca
    45781 gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa aaacatcaac
    45841 cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa aaataaagaa
    45901 tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag gaaacaagaa
    45961 taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg aagccaatcg
    46021 ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat agttccagct
    46081 accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact acttcagtct
    46141 cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca agtacatggg
    46201 acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc tcaagcgaaa
    46261 caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt acaagtccgg
    46321 atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat aacacgcaaa
    46381 atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc caaacgtata
    46441 accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg gggtgcataa
    46501 attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata cgattttaag
    46561 tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa accaaaacta
    46621 tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta ctgttttaaa
    46681 tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc tttttgtgaa
    46741 gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata atggaaattc
    46801 gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt atcaattgaa
    46861 attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa atgaactcgg
    46921 tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact atgtcaagaa
    46981 aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca tggaaaaagt
    47041 agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat tgttcatcgt
    47101 cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc tgaaattgat
    47161 tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc aaagaagaag
    47221 acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg tttattcgga
    47281 ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga gaaagataag
    47341 acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa agaaagtaca
    47401 acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg aggtaagctg
    47461 tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct attttattag
    47521 ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac cctacattaa
    47581 gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg gatattatta
    47641 ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc ggtatgcctg
    47701 gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag tatgatgaac
    47761 cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga gaatatgctt
    47821 atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt gactttgaat
    47881 atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt tcaaaagaca
    47941 gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt ttccgttatt
    48001 ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg tgacatgttt
    48061 tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc tcactttttt
    48121 tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt ttttgttcat
    48181 aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc ccgttttttc
    48241 caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat gactaatttg
    48301 ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa tttcataaag
    48361 ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata gaaggatgag
    48421 attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc ggacattttt
    48481 cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg aagcatcggc
    48541 aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag ttccgcctcc
    48601 gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta gttcttcgcg
    48661 aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga tggaatcaaa
    48721 aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa cttattatag
    48781 cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc tagaaagaat
    48841 atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg agaaatatta
    48901 ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat cgaggaatca
    48961 agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt tatcactctt
    49021 tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata aaaaaagact
    49081 atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac gcagcaaaac
    49141 attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata tatgatcaaa
    49201 ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca aatttatggg
    49261 atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga gcaatagatt
    49321 taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa atcaagtata
    49381 ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt tttaagtatg
    49441 gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat tatgaagaac
    49501 aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt ttaggcgttg
    49561 tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa acagacgtat
    49621 ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat gaagcgcaat
    49681 tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg gatattccgg
    49741 ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga tcaaaatatt
    49801 tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc tgccacaaaa
    49861 aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc gatcaagtgc
    49921 aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt cacccatcaa
    49981 tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag accgttatga
    50041 agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc gttttatgca
    50101 gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc gttataaaga
    50161 agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg atgatgaaat
    50221 ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag tcttagaaga
    50281 aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata tcatcatgga
    50341 aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc ttttcaatat
    50401 gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag ctaacgttac
    50461 cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg tatattcgaa
    50521 attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg aatcgcaagg
    50581 gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag aagtagagat
    50641 cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg ctggcggaca
    50701 gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag ggatcgttgt
    50761 tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga aaatcttgcg
    50821 tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg ccaatcgaaa
    50881 atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt tcccgcaaaa
    50941 tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga ttttagcagg
    51001 gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa aactggaagc
    51061 tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct tcttttttag
    51121 aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga aaacaatgga
    51181 aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa cagcggttga
    51241 tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg ctaggatatg
    51301 cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct cgaccagaaa
    51361 cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta gaagtgatag
    51421 atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga aaaaattggc
    51481 atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa aatgctcaga
    51541 aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta atggatcagc
    51601 aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg gaactgatgg
    51661 atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac gatggattag
    51721 ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat ggaaagattt
    51781 ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag caagcatttc
    51841 cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg atccaagttt
    51901 atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa aaaacactta
    51961 tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact attgacaaat
    52021 agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa gaatgtatga
    52081 aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga attgcaagag
    52141 gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac agtatacgga
    52201 ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt aaaagggaga
    52261 ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa agagtatgtg
    52321 gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg accattaact
    52381 ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg attatccaca
    52441 gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact ttccggtgtt
    52501 ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac tgcagatcat
    52561 gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc tacgcgaatt
    52621 ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat gattttacga
    52681 ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt tgctattgat
    52741 cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata caaacactac
    52801 tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc agttcaatgg
    52861 gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc ggatcaagtg
    52921 cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc ggcggctaaa
    52981 ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga tttgattttt
    53041 gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt gaaaaaagca
    53101 gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct tttagtatga
    53161 tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta actaatcgta
    53221 atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa attgtaggaa
    53281 aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga tctttgggct
    53341 gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc ctctgaaaac
    53401 tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa atacgcagaa
    53461 ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt tgaaaattta
    53521 gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca gcctcactcc
    53581 ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga cacgatctta
    53641 ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa cttcagtggc
    53701 aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat tgattataat
    53761 gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg tgccagtgct
    53821 tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt aggcgcaaaa
    53881 ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca tcctaatcca
    53941 gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg ccctcgaggc
    54001 ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc ggtatttcct
    54061 ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc attcaaagaa
    54121 gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc aaaagcaatg
    54181 gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac agataatcat
    54241 ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga gagcattttg
    54301 gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact aagtccattc
    54361 aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt caaagaagaa
    54421 gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga tgataacgct
    54481 caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc gttgcataaa
    54541 aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct tttctgttta
    54601 ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat atgaggtgta
    54661 attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt atctcgttag
    54721 aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc tatgaactag
    54781 caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg aacaaatatt
    54841 ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc aatattcgtc
    54901 tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt catggagcag
    54961 gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt gcggttcgta
    55021 cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa tatccaacag
    55081 caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag tcaaaaaatc
    55141 tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg gctacagtca
    55201 tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg ctgtattatc
    55261 cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa aactattttt
    55321 tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa gaagagcgtg
    55381 ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat cttccttcag
    55441 caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct tatgcgcgca
    55501 aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc atccatgatt
    55561 ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg gcattatcta
    55621 cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact tcttttcaaa
    55681 aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat tttgttttac
    55741 aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt tcaagtgatc
    55801 gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg cggaacaaag
    55861 gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt atcacgggat
    55921 atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca aaaaacgttg
    55981 tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat ggttgacggt
    56041 atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg agatgaagaa
    56101 acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc acgtcagttg
    56161 tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat cgatgcattg
    56221 aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc tgagggagta
    56281 aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct agatgaaaaa
    56341 ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg tttgtttggt
    56401 acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc ttttttggtc
    56461 atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata taaaaaaacc
    56521 atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt agttaacgat
    56581 tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat caatctcaac
    56641 aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc cgtgaattgt
    56701 gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta cgatagttgt
    56761 atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca attataccac
    56821 gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt acaatcagcc
    56881 aaatagagta tataatttct tttggataag gccagcttag ctcagttggt agagcaacgc
    56941 actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag agaagacaga
    57001 tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg aaataaagta
    57061 tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa taaaacgact
    57121 agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat gatcgagttg
    57181 cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa aaggatgtgt
    57241 gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa tccaaagatg
    57301 attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat agcgagaaaa
    57361 tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa taataacggg
    57421 ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg ttgcaaaaat
    57481 accgagactg cggcactgat tactacattg ataaaaaaat atttggctat atttttgaca
    57541 ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac aagtgtaaat
    57601 gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc aaatagcaac
    57661 cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag ttctattttt
    57721 tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa gatagagtca
    57781 gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat tgctaaaaat
    57841 ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt ctagcaaaac
    57901 caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt acttcaatca
    57961 tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa aagctagcat
    58021 tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca aaatctgcgc
    58081 ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat aaatttacat
    58141 agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc aatttagtac
    58201 ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc aatatacagc
    58261 ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga aaatcattgg
    58321 aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag attatttggt
    58381 tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg ttttcataga
    58441 tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa atcatattta
    58501 attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac attagcagaa
    58561 aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt gaaacgttct
    58621 tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt agaataaaaa
    58681 tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata acagtgtatt
    58741 gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt atactttgtt
    58801 tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc taaaagtgaa
    58861 gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga gatcaaagaa
    58921 aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca gtattatcct
    58981 aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg agaattgtct
    59041 aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga aaaagaatta
    59101 ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg agttattcat
    59161 ctcaatgaat taagaagtat agtcaatctc ataatgcagg ctaggttcat ttatttgaac
    59221 agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat tgtattttat
    59281 cct
//
LOCUS       DAJSNV010000009        58972 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSNV010000009 DAJSNV010000000
VERSION     DAJSNV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.009"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..187)
                     /locus_tag="ON516_000497"
     CDS             complement(59..187)
                     /locus_tag="ON516_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3344008.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ON516_000498"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ON516_000498"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3344009.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ON516_000499"
     CDS             complement(1674..2729)
                     /locus_tag="ON516_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3344010.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ON516_000500"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ON516_000500"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3344011.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ON516_000501"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ON516_000501"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3344012.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ON516_000502"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ON516_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3344013.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ON516_000503"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ON516_000503"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3344014.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ON516_000504"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ON516_000504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3344015.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ON516_000505"
     CDS             10491..12050
                     /locus_tag="ON516_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3344016.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ON516_000506"
     CDS             complement(12161..14092)
                     /locus_tag="ON516_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3344017.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ON516_000507"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ON516_000507"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3344018.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ON516_000508"
     CDS             complement(15021..15773)
                     /locus_tag="ON516_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3344019.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ON516_000509"
     CDS             complement(15932..16141)
                     /locus_tag="ON516_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344020.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ON516_000510"
     CDS             complement(16152..17480)
                     /locus_tag="ON516_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344021.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ON516_000511"
     CDS             17616..17771
                     /locus_tag="ON516_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344022.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="ON516_000512"
     CDS             complement(17845..18432)
                     /locus_tag="ON516_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3344023.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ON516_000513"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ON516_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3344024.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="ON516_000514"
     CDS             complement(20012..21301)
                     /locus_tag="ON516_000514"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3344025.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ON516_000515"
     CDS             complement(21455..22387)
                     /locus_tag="ON516_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344026.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ON516_000516"
     CDS             complement(22544..23203)
                     /locus_tag="ON516_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3344027.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ON516_000517"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ON516_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3344028.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ON516_000518"
     CDS             complement(24244..24534)
                     /locus_tag="ON516_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344029.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ON516_000519"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ON516_000519"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3344030.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="ON516_000520"
     CDS             complement(26821..28176)
                     /locus_tag="ON516_000520"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3344031.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ON516_000521"
     CDS             complement(28200..29369)
                     /locus_tag="ON516_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344032.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="ON516_000522"
     CDS             complement(29366..30253)
                     /locus_tag="ON516_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3344033.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ON516_000523"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ON516_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3344034.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ON516_000524"
     CDS             34320..34841
                     /locus_tag="ON516_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3344035.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ON516_000525"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ON516_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ON516_000526"
     CDS             complement(36284..36940)
                     /locus_tag="ON516_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344036.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ON516_000527"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ON516_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3344037.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="ON516_000528"
     CDS             complement(38477..39475)
                     /locus_tag="ON516_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3344038.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ON516_000529"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ON516_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3344039.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ON516_000530"
     CDS             complement(40491..41222)
                     /locus_tag="ON516_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3344040.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ON516_000531"
     CDS             complement(41226..42491)
                     /locus_tag="ON516_000531"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3344041.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ON516_000532"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ON516_000532"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3344042.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ON516_000533"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ON516_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3344043.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ON516_000534"
     CDS             47833..49335
                     /locus_tag="ON516_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3344044.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ON516_000535"
     CDS             49337..50074
                     /locus_tag="ON516_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3344045.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ON516_000536"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ON516_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3344046.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ON516_000537"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ON516_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3344047.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ON516_000538"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ON516_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3344048.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ON516_000539"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ON516_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3344049.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPIILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="ON516_000540"
     CDS             complement(55574..56740)
                     /locus_tag="ON516_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3344050.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ON516_000541"
     CDS             complement(56756..56983)
                     /locus_tag="ON516_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3344051.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="ON516_000542"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="ON516_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58434)
                     /locus_tag="ON516_000543"
                     /pseudo
     CDS             complement(57969..58434)
                     /locus_tag="ON516_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16238 a        12789 c        10264 g        19681 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatgatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct ta
//
LOCUS       DAJSNV010000010        53378 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSNV010000010 DAJSNV010000000
VERSION     DAJSNV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.010"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..314
                     /locus_tag="ON516_000544"
                     /pseudo
     CDS             12..314
                     /locus_tag="ON516_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            562..804
                     /locus_tag="ON516_000545"
     CDS             562..804
                     /locus_tag="ON516_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344052.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(909..2117)
                     /locus_tag="ON516_000546"
     CDS             complement(909..2117)
                     /locus_tag="ON516_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HCR3344053.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2407..3372
                     /locus_tag="ON516_000547"
     CDS             2407..3372
                     /locus_tag="ON516_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344054.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3394..3606
                     /locus_tag="ON516_000548"
     CDS             3394..3606
                     /locus_tag="ON516_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3344055.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3681..4157
                     /locus_tag="ON516_000549"
     CDS             3681..4157
                     /locus_tag="ON516_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCR3344056.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4154..4654
                     /locus_tag="ON516_000550"
     CDS             4154..4654
                     /locus_tag="ON516_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HCR3344057.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5214..7031
                     /locus_tag="ON516_000551"
     CDS             5214..7031
                     /locus_tag="ON516_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3344058.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7137..8465
                     /locus_tag="ON516_000552"
                     /pseudo
     CDS             7137..8465
                     /locus_tag="ON516_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            8542..8703
                     /locus_tag="ON516_000553"
     CDS             8542..8703
                     /locus_tag="ON516_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HCR3344059.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8744..9124
                     /locus_tag="ON516_000554"
     CDS             8744..9124
                     /locus_tag="ON516_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HCR3344060.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9139..9420
                     /locus_tag="ON516_000555"
     CDS             9139..9420
                     /locus_tag="ON516_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344061.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9679..10461
                     /locus_tag="ON516_000556"
     CDS             9679..10461
                     /locus_tag="ON516_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR3344062.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10634..12352
                     /locus_tag="ON516_000557"
     CDS             10634..12352
                     /locus_tag="ON516_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR3344063.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12354..13208
                     /locus_tag="ON516_000558"
     CDS             12354..13208
                     /locus_tag="ON516_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3344064.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13884..14204
                     /locus_tag="ON516_000559"
     CDS             13884..14204
                     /locus_tag="ON516_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344065.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14228..14638
                     /locus_tag="ON516_000560"
     CDS             14228..14638
                     /locus_tag="ON516_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3344066.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14785..14991
                     /locus_tag="ON516_000561"
     CDS             14785..14991
                     /locus_tag="ON516_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344067.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15338..15859
                     /locus_tag="ON516_000562"
     CDS             15338..15859
                     /locus_tag="ON516_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344068.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTRSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15917..16342
                     /locus_tag="ON516_000563"
     CDS             15917..16342
                     /locus_tag="ON516_000563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3344069.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16521..16751
                     /locus_tag="ON516_000564"
     CDS             16521..16751
                     /locus_tag="ON516_000564"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCR3344070.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16785..16967
                     /locus_tag="ON516_000565"
     CDS             16785..16967
                     /locus_tag="ON516_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HCR3344071.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            17080..18591
                     /locus_tag="ON516_000566"
     CDS             17080..18591
                     /locus_tag="ON516_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344072.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18914..19138
                     /locus_tag="ON516_000567"
     CDS             18914..19138
                     /locus_tag="ON516_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HCR3344073.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19131..19697
                     /locus_tag="ON516_000568"
     CDS             19131..19697
                     /locus_tag="ON516_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HCR3344074.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19685..19915
                     /locus_tag="ON516_000569"
     CDS             19685..19915
                     /locus_tag="ON516_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344075.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20116..20481
                     /locus_tag="ON516_000570"
     CDS             20116..20481
                     /locus_tag="ON516_000570"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HCR3344076.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            20481..22208
                     /locus_tag="ON516_000571"
     CDS             20481..22208
                     /locus_tag="ON516_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR3344077.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22238..24154
                     /locus_tag="ON516_000572"
     CDS             22238..24154
                     /locus_tag="ON516_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3344078.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24839..25021
                     /locus_tag="ON516_000573"
     CDS             24839..25021
                     /locus_tag="ON516_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344079.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            25048..26745
                     /locus_tag="ON516_000574"
     CDS             25048..26745
                     /locus_tag="ON516_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3344080.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26770..26901
                     /locus_tag="ON516_000575"
     CDS             26770..26901
                     /locus_tag="ON516_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344081.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26993..27856
                     /locus_tag="ON516_000576"
     CDS             26993..27856
                     /locus_tag="ON516_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344082.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27893..28429
                     /locus_tag="ON516_000577"
     CDS             27893..28429
                     /locus_tag="ON516_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCR3344083.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            28443..28865
                     /locus_tag="ON516_000578"
     CDS             28443..28865
                     /locus_tag="ON516_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3344084.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28795..31194
                     /locus_tag="ON516_000579"
     CDS             28795..31194
                     /locus_tag="ON516_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3344085.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31226..33217
                     /locus_tag="ON516_000580"
     CDS             31226..33217
                     /locus_tag="ON516_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3344086.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33240..33491
                     /locus_tag="ON516_000581"
     CDS             33240..33491
                     /locus_tag="ON516_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCR3344087.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33481..34710
                     /locus_tag="ON516_000582"
     CDS             33481..34710
                     /locus_tag="ON516_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCR3344088.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34707..36788
                     /locus_tag="ON516_000583"
     CDS             34707..36788
                     /locus_tag="ON516_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR3344089.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36937..40857
                     /locus_tag="ON516_000584"
     CDS             36937..40857
                     /locus_tag="ON516_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCR3344090.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40858..41802
                     /locus_tag="ON516_000585"
     CDS             40858..41802
                     /locus_tag="ON516_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344091.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41806..42009
                     /locus_tag="ON516_000586"
     CDS             41806..42009
                     /locus_tag="ON516_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCR3344092.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42309..42545)
                     /locus_tag="ON516_000587"
     CDS             complement(42309..42545)
                     /locus_tag="ON516_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344093.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42728..43114)
                     /locus_tag="ON516_000588"
     CDS             complement(42728..43114)
                     /locus_tag="ON516_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCR3344094.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(43096..44424)
                     /locus_tag="ON516_000589"
     CDS             complement(43096..44424)
                     /locus_tag="ON516_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3344095.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44385..44714)
                     /gene="mobC"
                     /locus_tag="ON516_000590"
     CDS             complement(44385..44714)
                     /gene="mobC"
                     /locus_tag="ON516_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR3344096.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44972..45343)
                     /locus_tag="ON516_000591"
     CDS             complement(44972..45343)
                     /locus_tag="ON516_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344097.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45488..45673
                     /locus_tag="ON516_000592"
     CDS             45488..45673
                     /locus_tag="ON516_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3344098.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            45831..45998
                     /locus_tag="ON516_000593"
     CDS             45831..45998
                     /locus_tag="ON516_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344099.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            46616..47275
                     /gene="vanR"
                     /locus_tag="ON516_000594"
     CDS             46616..47275
                     /gene="vanR"
                     /locus_tag="ON516_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCR3344100.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47278..48621
                     /gene="vanS"
                     /locus_tag="ON516_000595"
     CDS             47278..48621
                     /gene="vanS"
                     /locus_tag="ON516_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3344101.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48792..49598
                     /gene="vanY"
                     /locus_tag="ON516_000596"
     CDS             48792..49598
                     /gene="vanY"
                     /locus_tag="ON516_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCR3344102.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGGCLEEYLKPEK"
     gene            49616..50443
                     /gene="vanW"
                     /locus_tag="ON516_000597"
     CDS             49616..50443
                     /gene="vanW"
                     /locus_tag="ON516_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCR3344103.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50440..51411
                     /locus_tag="ON516_000598"
     CDS             50440..51411
                     /locus_tag="ON516_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCR3344104.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51404..52432
                     /gene="vanB"
                     /locus_tag="ON516_000599"
     CDS             51404..52432
                     /gene="vanB"
                     /locus_tag="ON516_000599"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCR3344105.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52438..53046
                     /locus_tag="ON516_000600"
     CDS             52438..53046
                     /locus_tag="ON516_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCR3344106.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(53112..53285)
                     /locus_tag="ON516_000601"
     CDS             complement(53112..53285)
                     /locus_tag="ON516_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344107.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        15301 a        11869 c        13965 g        12243 t
ORIGIN      
        1 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
       61 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
      121 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
      181 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
      241 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
      301 atttttcctc aaaattttag gttatcgctg aaagccccgg aaatacgggc atttcgcagg
      361 atatgagaac tcaatttact accaatttac tactttcgga ttgagccgca tatatgctga
      421 accatactgc taatacccgc caccccaaat acggcacatc ataaattgaa tacgacaccg
      481 taaacgcggc gttcctcttt cccgtcatgg gagaacagga cgccgctttt ttatgcccaa
      541 atagagaaag gaggcgacgc catgctattt acttccggcc ctgaccgggc gtttgaacgg
      601 ctgatgcagg aaaagcccgg tttcgatcac tacgacggcg gcggggcgga cgcgccggag
      661 gattgcggca cctgccgatt ttaccgcccg gattggaaat atcaattctg ccagtttgca
      721 gagtgtccct atcagccggg caagctgacc gactatgcag cggtcaaatt cacagtgaaa
      781 gacctgctaa taaaagtcaa gtagaaattt cagattttta gggaggcgaa aaaatgcaat
      841 acaaaatcaa gccgctgagc atctcaaaat taaaacggcg gccagccaga tggtatagag
      901 tgtaaaaatc agtcgtgctc cagggattcc aagtaggctt tcacagaagc gtagtagatg
      961 cgcaggaact tgttggcaga ggccatcatg tagacacgat atggcttgcc ttcagagcgt
     1021 ttcttgtcca tgaactggta caccggctcg tctattggag cacattgcag gatgacgctc
     1081 atcaccagaa agagcgtcct gcgcagtgag gcagatcctc gcttggaaat gctacggcta
     1141 cggacatcta tttgaccgga ttggtagggc ggggcgtcaa tacccgcaaa ggcaaccaac
     1201 gcttttttgg aatgaaaacg gcgtacatcg ccaatttcag ccaggagttg agggccgagg
     1261 gttgggccaa caccaaacat ccccatcact acaggatact ccggcagaga agctgccagg
     1321 gattgcatct cctgtttgag agcagctaac gcggaagaag ttgtctggag ttgagaaatg
     1381 gcctgttcta ccaaaagttt tgccgtatcc gttttcggta tgacaccgaa gtgtctgcaa
     1441 gcagaggcat aaatatccag cgccttatcc tcgctgaaat tgtagccgtg ctttctgcac
     1501 cacttctggt attgggtagt aaaggccttc tcagaccggc cacaaacgca ctcgcaatgc
     1561 caaaaagtag cgacaaagtc cacccacttc tcgctgccat cggcgcgggg cggactggta
     1621 aacaagcgat ttgcgtctgg gaaggtggtg tccagcaggg agatcaggtt attcttcagc
     1681 atggtctgaa ctttggaata ctgttggtac tgccggtagc agattttcag catgagtcgg
     1741 gtatcctcct ccgggatata tctcggcaat gtgagccagt ggtcaagacc gtagttggcc
     1801 agctttacgg catccttttt gtcggttttg gcccgtctta aactgttgtt tccgtagtca
     1861 tgcaccagca ttgcattgac taccgagaca taaaggcccg cgtcgtggag cagccaggcc
     1921 accggagcat ggtaattacc cgtggattcc atcaccacac gggtctcgcc gtccaggctt
     1981 ttgagcagtc ctgccagctc gctcagttca ttggcggtgt gacgtacttc aaagggtgaa
     2041 accactacct ctccgaaggg ccgcatgact gcaatcatgc tcttaccctt ggaaacatcg
     2101 atgccaacgc agttcattca cattcctcca atacaaagaa tctgcaaccg gaatccatct
     2161 tttctcgctg ccgattcaat ctattgtgtg acacgaactc tccgacatcc ggtggttcaa
     2221 cctgcttaaa tcgaacgctg caacgagagg atggctaaca gtctttcaca cggacataca
     2281 agcccaagga gtgattggtc agccagttgc tcctctcatt gtagcttagg cacgagatgg
     2341 agggaaagcc ggactggctg tccggctttc ctgtccaaat ttattgtaat aggagcgaac
     2401 aacgctatgg cagttttccg tgtggaaaag aacaaaggct atacggtgat gtcaaatcac
     2461 cacctgcgga ataagaacct gaccttaaag gccaagggcc tgctgtcgca gatgttgtcc
     2521 ttgccggagg attgggacta taccctaaaa ggactgtcct atatcaaccg ggaaagcatc
     2581 gacgcgatca ggacagcggt atgggagctt gaaaaagctg gatatatccg gcgtgagcag
     2641 ggccgtgacg caaaaggcaa aatggccgat atggtctata ccatctacga acagccggta
     2701 ttggaaaacc cggtattgga aaatccaaca tcggataacc cggtgttgga aaatccgaca
     2761 tcggataatc cggcgtcgga aaatccaacg caattaaata aagaagtaca aagaactgac
     2821 ttatcaaaaa aagaaaaaat aattacagat ggacaaagta cccattccat tcctatccca
     2881 tccccgaccc ctgcccctat ggagggcgag gcagcggcta cgccgccgga acggaaaagg
     2941 acaggaaagg atgaggctgt acggatttat cgggaaatca ttttggagaa catcgagtat
     3001 gcctatctca ttcaggacag gagcattgac cgtgaacagc ttgacgaaat cgtagacctg
     3061 atgctggaaa ccgtctgcac cgcccgaaag acaatccgtg tcgccgggga cgactaccct
     3121 gccgagctgg tgaaatccaa gtacatgaag ctgaccggcg aacatatccg ctttgtcctt
     3181 gactgtctgc gggagaacac aacgaaagtc cgcaatatca aacaatacct gcgggctatg
     3241 ctgttcaacg cccccagcac catttccaac tactacacgg cccttgtcgc gcacgacatg
     3301 gcacagcccg attggggaaa gcctaaatcc ggcctgccgg actattcctg cggcgagggc
     3361 gaaagcctgt aacgagaaaa ggaggacgac cctatgaagc agggcgcatt gatttttgac
     3421 gagcgaacag accgttacga catccgcttt gatctggccg actattacgg cggcctgcat
     3481 tgcggggagt gtttcgacgt gatgatcggc ggcaaatgga ggcccacccg cattgaaatg
     3541 gccgaaaact ggtatcttgt cggcatccgg gccgacgacc tttccggcct gcgggtgcgg
     3601 atttgaccta tatctacctg cggcgtctgg tggttccgcc ttagacgccg cttttgactt
     3661 tctgacaagg agggatgcga ttgcaggaag aagttactca aaagaccatt gccctgtcca
     3721 tgaaaacggg caagctcacg gctcaagtgc tgcaagcggc gctgaaaaag tacctgcaac
     3781 accgggccaa gggcaaaaac acgctgcacc acggccagca gagcctaaag cagcttaaaa
     3841 agcatggggc tgatctctcc aacattgaga tcaccgaggc caacattgga gcgttcaagc
     3901 catgcgccaa gaaatacggc gtggatttta ccttacgaaa agataagacc acccagccgc
     3961 cccactacgt tgtgattttc aaggccaagg atgcagataa ccttgatctg gcgtttcggg
     4021 agtttaccgc caagacgctt tccaaggagc agcggccctc tatccgcaag gttttgtccg
     4081 ctgtgaagca aaaggcggcg gcccagacca agcagcgggc caaggagaaa atcaaggaaa
     4141 gggggctgga actgtgaagc ctgacgtaaa gaaattgctg atcctgaacc tcccgtatct
     4201 gatttttgtg tacctgtttg cgaaatgcgg ggaggcgtac cggctggccg ctggtgctga
     4261 cctgtcgggg aagctcctgc attttgcgga tggttttgcg gcggcttttg ccaacccgct
     4321 gccaagcctg cacctgtttg acctgtgtat cggtatcgcc ggtgccgtga tcgtgcggct
     4381 ggtggtgtac tgcaagagca agaacgtgaa gaaataccgc aaaggcatgg agtatggcag
     4441 cgcccgctgg gggacggcca aagacatagc gccttacatc gaccccaagt ttgaaaataa
     4501 tatcctgctc acccaaacgg aacggcttac catgaccggc aggcccaaag accccaagac
     4561 agcgcggaat aaaaacgtgc tggtgatcgg tggcagcggt tcagggaaaa cgagattttt
     4621 cgtgaaaccc agtgcgcccg taagggcggt ataaatccta ccttgagcaa gtagtaggta
     4681 aaagtatcaa gcggcattgt gccgcaacct atcattcccc gtcttacccc aaaagggaaa
     4741 ccggagggcg accatagcat gacggaggac acggggcgct gaagcctcag aagcgccggc
     4801 gtgacggaat gaaaatccac gtatgaggat ggaagctaga ctgcttgaat gacagcctga
     4861 aatcgggacc aagagcgtac ccctgacgta gagaggttgt actcgtcagt gttcctgaca
     4921 gtcgcattgt ttggctatgc ggctgctgat actcggagca ggttcagcca cgggaaagtg
     4981 gaaaaaggcg aacgtcacat accgacaacg tggaacgctt gtttataccg tactaaacgg
     5041 ggattgccta aagtgaaatg ccgaaaggct atgaaaacgc tgaaatgcgg ggttctgaat
     5101 attcaccatg gcaacagagt tcccatagta gtctgcggac gggaaagccg tctacatggc
     5161 aaaggggaac agtgaatcat tttcaaaaca gaaaggatgg tgtgtgagac actatgagaa
     5221 atccgattca tgtcttgaaa agccttgaag aaaaggcaag tgtaagtaac tacaaatatg
     5281 aaagattata tcgcaaccta tacaatccag agttctatct ccttgcatat gcgaacattg
     5341 cgaaatcgca ggggagcatg acgcaggggg tagacgggca gacgctggac aacatgagcc
     5401 tgccgagaat taaccggatt attgaatcca tacgcaacag gacatatcag ccaaagcctg
     5461 caaaaagaaa gtacattcct aaaaagaacg ggaaacttcg cccgttggga atcacttcta
     5521 ctgatgataa gttagtgcag gaagtggtca gaatgattct tgaagcaatc tatgagccga
     5581 cattcagcaa caattcacac ggtttcagac caaaaagaag ctgtcacacg gcacttacgc
     5641 aagtgaagaa aaacttcaca ggtgttacat ggattgtcga gggagacatt aaggcgtgct
     5701 ttgataactt cgaccatcat gtgttggttg aattactgcg gaaaaggatt tcagacgagg
     5761 cttttatcgg tctaatctgg aagttcctga aagccggata tatggaacaa tggcagtaca
     5821 actgcaccta ctccggtgtt ccgcagggca gcggtatcag tccgatatgt gcaaacatct
     5881 acctcagcga actggacaac tatatgcagg aatacaaaga aaagtatgat tgcgagccag
     5941 aacgcagaag gacgaccaga gaatacgagc gggcgtcccg gagatacaga aaggcacgta
     6001 aagcgttaat gggcgcagaa aaatcaactc ctgaactggt aaaagaattt aaggattcca
     6061 gaaggaagaa gatgaatcag cactattaca atccgtttga ggaaggcttc aagaaaatcc
     6121 agtacaaccg ttacgccgat gattttgtaa tcggcgttat cggctccaaa aaggacgcag
     6181 aaaaaatcaa ggaagatgta aaaatctttc tccaagaaaa actgcatttg gaaatgtccg
     6241 aggaaaagac gaaagtcacc cattccagta agccggtacg ctatctgggg tacgatttca
     6301 aagtaatcca ttccaagaac atgaagcgtt gtaaaaacgg cgacatgaaa cgggtgtggt
     6361 atggaaaagt attcctgtat atgccgaaag aaaaatggat taaaaaagcg atggaacggg
     6421 gagcgataca ggtgaaacgc aacaatgaca caggcaaaga aatgtggcgg cctatgccaa
     6481 ggaaagacct gatgaaccgc agcgacgcag agattgtgtc tactttcaat tccgaaattc
     6541 gagggttata taacttctat cggatagcag aaaacgtagg cgcattgcac aagtattact
     6601 acatggtgcg gtacagcatg ttaaaaactc tggcaggaaa gcacagaacg aatgtcagtg
     6661 ttattaagaa gcggcacatg gtaaacggag tactgagaat cccatacgat acaactaagg
     6721 gacgtaaata ctgcgaattt taccatgatg gattcagaaa gcacagcgac ggctatgaca
     6781 atgtggcaga cgttatgccg agttatagga aatatgacag caggcacacg atagtaaacc
     6841 gcataaaagc cggagtatgc gagatttgcg gggaacatgc agactattta tgtatgcacc
     6901 acgtaagaac gctgaaatcg ctgaaaggca gggatatatt tgagcagaaa atgctgaaaa
     6961 tgagaagaaa atctctggct ctctgccctg actgctttga actcttacac gaaaccaaag
     7021 aatcaaggtg attcatggaa agccggatac gctgagaggt gtacgtccgg tttgggaggc
     7081 ggctccctga aacctactga tgaaagtcag caaggcgcag ggtgcctacc tcatacctga
     7141 tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat ccgaaaggca
     7201 ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt aaaatcctga
     7261 ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc cattcggaaa
     7321 aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag ggggaaaagt
     7381 cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg atcggctata
     7441 tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa atgataaacg
     7501 ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc atgtttgagc
     7561 gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt
     7621 ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt gcgccctttg
     7681 acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac accatcgggg
     7741 acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt aatttcattc
     7801 tgactatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg
     7861 gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac attggccaaa
     7921 tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg gcctctatta
     7981 ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag atcatttcgg
     8041 ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag gagatttccg
     8101 atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg ggaagccaaa
     8161 cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa gacgaaattg
     8221 ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg
     8281 agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc gacaagaaga
     8341 atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag caagtggtaa
     8401 cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg
     8461 aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat gagccggaaa
     8521 ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac tttgcagact
     8581 atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct gttggaggga
     8641 tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcacttaat gtcttctatg
     8701 tgaaaagatt ttaaagagaa aatgcacatc ctacgcagat aacatgacaa tttcatgcaa
     8761 agcgaaagga gacaccatga aaatcgaata cacaaaagtg ggagactatt atttaccgaa
     8821 tctttactac ccggaggaaa ccagaccgat tggcttatgg ggaatgcttc ggaaggaata
     8881 tctgaaagaa cataaatctg gaacttatac atatcttctt ctgaccgcca ggctggacag
     8941 ttatttggca gatctgaatg aacaggcaca ggaacgcttt gaattgatcg aaactcagat
     9001 gcgaagtgcg gagggagttt ccgaggaact gaagagacag aatcctatgg aatggctatg
     9061 ccactgtaac aatatccgca accgggctgc tgagatcatc aagcaggaac tgatttatgt
     9121 ataaggggga ttctagatat gagaagagaa ctgattgaaa gttattatga agaataccag
     9181 cgggagctgg aaagatttct tgaatctgac ccggagatgt tcactctgga agcagaaatt
     9241 catcattaca tagaaatact ggaaccggtc ctcagagatg gcggacagga gatgtggcag
     9301 acactggaca ggattatttc agcgaaaaat gcaatggagg gaattgtggc aaaagaaatg
     9361 tatatccggg gatttctgga ttatgaaaga ctggtgtgtg aaaaagacag taagatctga
     9421 ataatcagaa catagactat atataacgag gcttttattg tatagacagt aagagcctca
     9481 tttaatatag ttttcataat aaaaaacagg tggtagtacg tatttgcttt gtgttatata
     9541 agaattggtc tggctgaaca atggtgaaaa tttactatga ttttacttaa atctgattga
     9601 aggatgaaaa agtgatgata tcatatagtg ggttgatagg gttacagaaa atgtattcac
     9661 agaggcagag aagaaataat gaaagtaaaa aatgcagatg aattttataa aatattagaa
     9721 agaaaggcta tatatccggt atttcagccg attgtaaatt tacagactgg agatgtggca
     9781 ggatatgaag ctcttagtcg tatcgacagg catgatacga ctcttatgat ttcagatttg
     9841 tttgtgattg ctgaacaggt aggctgtgtt tggaaattgg aaaaattgtg cagaaataaa
     9901 gcattgaagg cagcggcgaa taaaccagaa catgcaaaat tatttcttaa tgtagatggt
     9961 aatatcattc aggataaatc gttcatacag ggatttacaa accgtaaggc tgcgaaagcc
    10021 ggagtaccgt cctgtgacat tgtatttgaa ataacggaac ggtcggatat agaaaactat
    10081 cagattctgc aacagattat gaagcattat gcagatcagg gatatgaaat agctttggat
    10141 gatgttggag ctggatattc tggtctgaac cgtgtagtaa atacatcgcc aaactactta
    10201 aaagttgata tagaactggt cagagatatt caaaaacata agaaaaagga aattatgatg
    10261 gaatttctgt tgcattactg taatgagaca ggagcaattc tgattgcaga agggatagaa
    10321 acagaagcag agttggagtg tctgtgtaga ttaggtgttc attacggaca aggatatttt
    10381 cttggaagac cggacagaac gttcaaaaat gtaaggaatg aggcgatgtt ggtattacgt
    10441 agactgaata ataaatcgta atgaaaacta tatgtcagca ggtgaaaaat gaataagtca
    10501 attataacat tataaagatt tccattgatt tttacaccta ttttacacaa gcactcacat
    10561 agatttgatt tctgtcttct ataattgtat aggaaaatgt agatcttata taataacagg
    10621 agaggaaaga gaaatgaacg agaatgtgaa agtggttttt ggtctgatcg gtggccttgc
    10681 cttgttcctt tatggaatga acagcatgag tgatgcactt cagaaagccg caggagaacg
    10741 aatgaaaaaa atccttggat ttcttacaag aaatccaatt atgggagcac ttgccggagc
    10801 acttgtaaca gctgtattgc agagcagttc agcgacgaca gttatggtta tcggttttgt
    10861 cagtgcggga cttatgagtc ttccgcaggc aatttctgtt atctttggtg ccaacatagg
    10921 aaccactatg acggcacagc tgatggcatt taaaatcagc aattatattt atccaattat
    10981 ctttgttgga tttattctga actttgttag caaaaaagaa aaggttaaaa atatcggtat
    11041 ggtaattttt tcattcggac ttttatttga aggaattgaa atcatgggtg aagtcatgaa
    11101 acctttggcg ggaagtccgg tctttgttga tctgatggga aaagtttcat ccattccggt
    11161 tcttggtgtt gtactgggag cggtcatgac attggttgtg cagagtagtt cagctacgat
    11221 tgcagttctg cagaattttg catcacaggc aggaccggac ggagtaagca gtgtaatagg
    11281 gcttaccggt gcgattccga ttcttcttgg agataatata ggtacaacga tcacagcact
    11341 tcttgcatca attggacaat ctaaaaatgc aaaacgtaca gcaattgcgc atagtatttt
    11401 taatatcagt ggaagctgcg tttttatttt cctggttccg tggtttgcaa aatttgtcca
    11461 gtttatttca ccaaagggaa atgagattga tgtgatttca agacagattg caaatgcaca
    11521 tacaacgttt aatatcgtct gtacactggt ctggctgcca ctgattccgc tgatggtaaa
    11581 aattgttaca acaatcatcc gtggaaatga caaaacagag aaagcagctt ttgaacccaa
    11641 atatctggat atgaaagtga ttgaacagcc ggcggctgct atggttctgg tatctaagga
    11701 actgaaccga cttggagaac tggcagaatc tctgttaagt gatttaaaaa cggcgattgt
    11761 agcagatgga gactcaaaaa ctcatggttc gtttatagaa aatctggaga ttgtacatca
    11821 gttacaggac agtgtgacag aatatattac cagattattc gcaagcggaa accttacaga
    11881 acaacagtct gagcagacgg cagggttact gtatgtaaac aatagtattc agaggattgc
    11941 agacagatgt gaagatattg accagatatg tgaaaaagta aataacaatg gaaaaagact
    12001 gaccagtgaa gcctcctcag aaatggaaga ctgcattgat attatccagc agcttttaaa
    12061 gaaagcaatg gaggcaatcc gtaagggtga tgctgtagtg gctgaaacag tttttaaaaa
    12121 taagaaaaaa atgcataaag cagagaaaaa atacagtaag gcacatttaa accgtgtaaa
    12181 aaatgaggtt tgtgatgcgt ctatgacaag atatttttct gggatcatgt ataattttga
    12241 ccgtatggca gataattgtg tcagcattgc agaagaagcc tgtgataatg tggcttttat
    12301 aaaccttgat gaagagacgg gaaaatcaat gatggaaaga ggtgcagtat aaaatggtaa
    12361 agaaaaaaaa catccgaaaa tctgcacttc tgctgataat gataatgctg gcaacgggaa
    12421 taacagcatg tggaaccgag gaagagccat tgccggatct gtctgctatg ggagctgtca
    12481 cagctgtatc acgagaagaa gggtcaggaa ccagagcaga atttgagaat cttttgaaac
    12541 tgcctgagag tgatacagga atagttgttg attctacaga gaaagttctg aaaaaggtgg
    12601 aagaagataa aaacgcagtt ggttatgtcg catatagttc cgcaacagat acgaatggaa
    12661 aaatacttca aatcaacgga gttcttcctt ctgagaaaac aatagacaat aacagttatc
    12721 ctctttgcag ggattattat ctggcttata atggagaact gacagatgtg gaacaggatt
    12781 tcctgactta tgtaaagagt aaaggacagg atattgtgaa acaatattgt attcaggctg
    12841 acagtacaac aacattcctt tctgacaagt cagaaggaaa gattctgatt gaaggatcca
    12901 cttcaatgga accaatggta aaagcactgg cagatgacta tcagaaacaa aatccaaatg
    12961 ctgaaataga agtaaaagca acagattctt caagagggat tacggcggtg ataagtggag
    13021 agtgtgattt cgcaatgtca tccagagaat taaaggatta tgaagcagag cttctggaaa
    13081 caaaagtcat tggaaaagat gctatagcta ttgtgataaa tgaagaaaat ccactggaaa
    13141 atcttacaat aaaacaactt acaggtctgt acaatggaac atataaaaac tgggatgatc
    13201 tttcctgaga aaaacgaata taatatctta caagcactga ctataacggt cggtgcttct
    13261 tttttggatt ttttcaaaat tatctgacat tcttcaaatt cccacgtgtg attagtggaa
    13321 aaataatttt ttctgcaaaa gtaccgtcca aatgccctct gaaacttgct attagtgagg
    13381 gaatatttca tttatgcgaa aaaaacttgc aaaagggggt tggtaaatac cgcttcccag
    13441 gctttatagg tagaggaaca attattttaa aaattttacc ccaaagatgt ccgcttttgc
    13501 ctgtctcagt acagagtatg tgaagggact aacaaaagta aaagaaaaaa tgataaaaat
    13561 gcaaaaaaat atcacgaatc ttttgcatct tgcgaagtca tttgactcta gtaatcagag
    13621 gacgttttgg aatgttcctt cgatgaacct tgacaaccga taagtgcacc ctagaagcct
    13681 aacaggagtt atgccatgac ctttcttttt ctgagagcga gcgaaattat aatgacactc
    13741 ccgatgatgg gcggctgacc gaagccttat ggtcagtgac ctgctgatgc aggcagaggc
    13801 agtctgagac ggctgcaggt gacagggaaa cgcacgccag accgccggac gaatgaaact
    13861 gatgacaatg tttacagggc aaaatgcccg gaaaggagca gtttatgaca gaactgaaaa
    13921 tgaaagaaat gcagttgatt aactggtttg gatgcccgaa tctgatttat acaagggaga
    13981 gaatccatct ggttgccatg cttacaacag atgaagaaat gaaaaagaat ctttacaggg
    14041 tatgcagatt ccttgcaaga gaagaaatcg catatcggta tccgatcatg tatgaaatga
    14101 tccggaaaat attttcaccg gatgatacca atccaccgga agcatgtgca atgtttatgc
    14161 cggacgatga aacagatgat gaagaatatg caatggcagc gtagctgcgg aaaggacagt
    14221 aacgattatg aataaagtgg ttttaatggg aagactgaca aagaatccgg agatcaaata
    14281 tgccggaaaa gataatgata tggcagtagc aagatataca ctggcggtaa acagaagata
    14341 taaacgtgac ggtgaacagg aagcggattt tatttcctgt gtcacattcg gaaagagtgc
    14401 agagtttgca cagaaatatc ttcacaaggg aatgaggatt gtgattggcg gtcgtatcag
    14461 cactggcaat tataaggaca aggacgggaa aacaatttat actacagatg tgatcgtgga
    14521 agaacatgaa tttgcccaga acaaggataa tggtgttgga gctgattcat cggaaacacc
    14581 gaaaacagat aaggacggat ttatggaagc accggaaggt gaggttccat tcgactaaac
    14641 cgaagtaaat ggttggagag gttgcccttt gtcggggcat cctcttttcc attttacaga
    14701 agacatcaaa aattacaggt attcaaccta acgaaatcgt gatataatca aaatatgttc
    14761 agatgaaaaa tatggaggtt gcttatgggc gtaagttata acagattatg gaaaatgctc
    14821 atagataaaa atatgaagcg gatagagatg cagtacctca ccgggatcag tggaaatatt
    14881 cttgccagga tgggaaaaaa ccagtatgta tcaatggaga cgattgagaa gatctgcaag
    14941 aagctggact gcaccgttga tgagatgctg gaatttacag atgatgagta acaagagcag
    15001 acatgtcaaa ttattaaaac tggaagaatg cttcagaagt cgaaaaaaga aattgaaaaa
    15061 agataacacg ccggatacgg cataaatctc ttagcaacgc taagagcgca cttctataca
    15121 ggcaattcca tctgcctgta tcgtgcgacc tgcggtgctg ttcccgattt caagaaatct
    15181 caaaccggga aatacagcga aatcagagat ttcgtacaag gggaagatcc ccttgcggca
    15241 cagatgccta tccctgataa agtttgtgac tgaatatttc agatatagac atataagggg
    15301 aaagaaagtg tgaaatcaaa atttgtggag agattttatg attagaaaag caatactaaa
    15361 agatgttaat tcgattgaag atacttacaa tgagcatttc cagtatgaac tgaatcatac
    15421 agcctttact gtatttaaga aaggggttta tccaacgaaa gatgatgcag aacgggctat
    15481 ttatgctggt gctttgtttg tatatgaaga aaacggaaca attgttggaa gtatcattat
    15541 tgataaagtt caaccaatag aatatgcaac tattccttgg aaagaaaaat tatctgaaga
    15601 tgaggtaatg gtaattcacc ttttaatggt acgtccgagt atgtctggta aaggaattgc
    15661 atcatcacta ataaaattcg ctacagaatt agcacaaaaa aatagttgca gggcactacg
    15721 tttggataca agaagtcaaa atattcctgc gctatctttg tatcaaaaaa atggatttga
    15781 aataattgct agtgcttcta aaaaagttgg agatgtaatt gcacacaaaa accatctttt
    15841 tctcgaaaag atattgtagt tcgtaaattc cagtttgaaa aattgagaaa atcggagttt
    15901 gaggagcatc accgttatga ttagagaatt acggaaagtg gatataaata aagttgcaga
    15961 aatatggtta gatacaaata taaagacaca ttattttatc tctgctcaat attggaaaag
    16021 taattttgag ttagtaaaag aacttctatt acaggcaact gtctatgttt acgaggataa
    16081 acaggaaata cagggtttta tagggttaag taacgaatac attgaaggta tttttgtttc
    16141 tgccgaaatg caatctcaag gtataggtaa aattttgcta aactatgtaa agggaaaaag
    16201 aaacaaatta atcttaaatg tatatcaaaa aaatacacgg gcaatatcct tttatcaaag
    16261 agaaggattt gagattcagt atagcggttt ggatgaagct actggagaaa aagactatgt
    16321 aatggcatgg cagcagaaat agaaattcaa gttttagtgt ttggaaaatt atgacttagt
    16381 gtgcaagttg ggagaaaata tggtaacgga aaaagagaaa aaagcatgtc gatttattgc
    16441 cgaatggcta taagattgtg agacagtctc tggtagatga tctggagggc gaaaaaaagg
    16501 tatgttggga gacagaaatt ttgcaggatg atgatagtgg atttacttat gaaatcagca
    16561 ggtgtctgta tttcgatacc tgtaaggaac atggctatgc agagttctgc aaagtatttt
    16621 gcaaccatga ctggtatgct tatggagtat taaagcatca ttcgagattt atcagaaaat
    16681 cgacgattgc cgaagatgga actgtatgtc atgatacaat cgagaaagta acagagaaac
    16741 attttttata aaaccgaaaa attgattatg ggaggaaatt gaaaatggcg aaagaagaga
    16801 gatttgaaaa aatatattct cagggagcat taactactac tgagatatgg gtggataagg
    16861 aaactggtgt gaattattta tatcatgcat gtgggtattc aggtggactg actccattgt
    16921 tggacagaga tgggaaacca gttatttctc aggctataaa tagatgacct tagaaacagc
    16981 aactgttatg gtaagtaaca gcatcgtcac aatatgtata tggtcgattg gtgtgtgcgg
    17041 taatatacta tattcagaaa aacaattcag gagattttta tgagaagatc aggttggaca
    17101 aaatggttga tcgtgattcc ggcaatgcta ggcattgtgt tggtgaccta tgtgcggtat
    17161 acaacggata tctggggatt tggtgcattt atctgccagc tgattgcaat tttggaattg
    17221 gcttatggtc tgcgaattgc gatgctggca cagaatagaa agaagtccta tcggttaaca
    17281 ccggaagaac gacatgagta tgcacggtat ttatatgaga agcaatatca ccgttaccct
    17341 gctgtagcga atcagatgct tttggttatg gcacggatga gtgtactcct taacaattat
    17401 gaacgggctg cacaggagct ggcagatatt cgtatagata aattcaatcc agcacagttg
    17461 aagttgtatt attacctgaa agtggttaca gcaatggcag caggagatgc gacagggatt
    17521 caagagagcc agatgtgtta cgcagggatt ccggatacta aatgcacata tccgtcaaaa
    17581 gcagagcttg ctatgtggat agagcagaga gcggcagcgc agatggcaga ggcactgaag
    17641 aaggcagtgc cggataaaaa agagcatccg atacggatca gtatcattac tttggttctt
    17701 gcgtatagta ccttcttcta tggattgtgg tatggtataa aacgtgatat gggatatgaa
    17761 gtgagatatc gtttcgcaga aatatcgatt actgttatta caatcagtct ggcggttctt
    17821 ttgatctggg ggatcgtgag tttatacaga cacaattgcc aggcattatc catacaattc
    17881 ggtaaaaaca aagtggcatt gataattgtc tatggagctg ctgcgatatt tgcacttttg
    17941 attattggaa tgagtggtct gagtgtagtg ttcggaatgg atggaacaga aacggtgact
    18001 ggttgtgaca gcaaatatac gtatataact gtgcaggaag attatggtgg tgaacggaga
    18061 tacagaacca ataatccatt tgttatgcag agtatggatg gtctgtggtg gtcttcggat
    18121 acaatagatg aagaagataa tcaagaagag gatactacag aattaccgga tttggataca
    18181 gagaattcaa cagattcaga ggatattcag agtggaaata ccatagaaga ttacgagaca
    18241 gaagggcagc atgatggtct gacaattcaa aatgcaatgc tggcagttta taattacctt
    18301 caggagcaga atacacttca aaatatggcg ttcagttaca cggcgaatgc aaagggagaa
    18361 gtctatgctg tcgtcagcga aacgcaggaa acaaagaacg gtaatacggt aaatgtgaga
    18421 tactgtctgt acgataatgg ttccaagaca gatgcagatg gtaattcctg cgaagaactg
    18481 gtgttagaaa aagtatatcc ggatggcaat tacgagaccg aattggtaga tttttatctg
    18541 gtaaatgcag atacgatgca ggtgacagac gaacagaaga atacgtggta aaataaattc
    18601 tggggaagta ggtgcattgt atgacaatga gagaaaaaat aaaactatta tcgtaaatgt
    18661 ttcaagcgga ctgggttctt ttggaagtat gcaaaaggac tccaagacat aagaggtgaa
    18721 ctgcgctgat ccaggtgcca cggcaacaga tttgaatgtc gacggaggaa agcaaacagt
    18781 acaggcgcaa tcgtaaaact tgcaatgatt gataaaaccg gactaacagt aacttttatt
    18841 agtcgatttg gaaatatgcc ttggtaatca acaattccgg ttttactgac taagaaaatg
    18901 aggaggattc ataatgagtg agaaaacaga gattactttt atgcaaacaa gactaatccg
    18961 gcttgcttca gaagagtggc atttacctgt tgaacagatt atccacttgt ttaaagaagc
    19021 cgatgttctt ggatatatag aaaaatgcta tgggattttt cattgtgaag gcgatgaagc
    19081 tgtgcttgaa gatattactg aatttttgca aagaaagggg atagagatta gtgcttgagt
    19141 taaaagatgg atttgttctc tatcatggaa gttattgtga agtaaaagag ccagatcttg
    19201 ccaaatgtgc aaagagaaaa gattttggac agggatttta tttaaccaca tcaaaggaac
    19261 aggcagaaag ttttctgaga acctcaatag ccaaagccat cgcaaccgga acaattgaag
    19321 aggggcagaa gtttggatat atttcaactt ttgaattcaa actttcagaa aatcttgaaa
    19381 cgcatatttt tgaaaatgca gatgtggact ggttacattg tatcgccgct caccgaaaga
    19441 aaaaaatgtt tattgaggtt gaacgtgaaa tggcaaaata tgatatcatt gcaggaaaga
    19501 ttgcggatga tgcaacaaac gctactctta ccgcttatct tgcgggtgct tttgggactg
    19561 caggtgataa agtagcagac gatttctgta tcagacagtt gctgccgaat aaattgaaag
    19621 atcaatactg ctttaaaact gaggcagcta ttgagtgtct gaaatttgtg aaaggtgaga
    19681 agatatggct gaagtaacaa ttccaaagga aaaaatgaat tatacaatcg accttctgat
    19741 cactatggtg acagatgaaa tcgcagaaga gacaggcaag gacagaaacg aggtattgac
    19801 agattttttg tgttcaaaga caggaaaagc actgtatgat gagaagacaa agctgtggtg
    19861 cactggtccg gcatatatag ctgaactgta tatggaagaa ttaaagaaat cctgatatgg
    19921 gatgtgagtt taaatttata cgacaatggt gacaaagact ttcattccaa ggagcaacaa
    19981 ttaaatattt caaaaacgct gaggtcagca tttcctaatc acaggacaac gacctcagtt
    20041 ttttataccc aggaatcggc attcatgaat aagccggttc tttttgttta cagaaaaaag
    20101 aaggagtaca agaaaatgag cagagcaaaa aatccaaagt atgaatccat gtcattggag
    20161 gaactgaagg ccaacatgga aaagagcaga caggaactgg aggcagcaat tcaccacaaa
    20221 aacattctgg agcagcggaa gaagctggta gaacgcaggg aacgttccca ccggctgatt
    20281 gtcaaagggg cagagtttga gaaagtattt ccgctgtcca gagatctgga acaggaagac
    20341 gtccaggaca tgatgaatca actgcagaac agcagttaca acaagagtat tgtgcggcag
    20401 gctcatatag ccgccttaca caaagatcaa cagcagattg cagaagcagt aaaaaagaca
    20461 gagaagggag atgacagtta atggcactgt atcatttttc agtaaggaat gtatccagag
    20521 ggaaagggca gatggtggtc gcttccgcag cctatatttc cgggcagagg atttttgaca
    20581 gttattacaa caagatacac gattatacga gcaagtcagg tgtgatattt acagaaatcc
    20641 tgacaccgga gtatgtgcca gagcgtctga ctgacaggga aacactctgg aacgaggtgg
    20701 agcatgtgga acggaacaac aaggcacagc ttgcctattc gtttgacatt gcactgcaga
    20761 atgaattgac acttgaggaa aatatcagac tggcaaaaga gttctgccag gagcagtttg
    20821 tggcacgtgg catgatcgtg gatctggcag tccatgaggg aaaagcggaa aatgaagaag
    20881 aaccggataa tccccatttc catgtgcttg tcccaatccg tccttttaca gaggaaggaa
    20941 tctggggcaa taagcagaga agggaatata ttcttgatga ggatggaaac cgggtgaagg
    21001 atgccaaagg aaaagatatg ttcaatgctg tttctactac cggatggaat gatccggagc
    21061 ttttaaaaga atggcggcgg gcatggacag agaaagtaaa tgggaaattc agggagtgcc
    21121 acatggcatc cagaatcgac caccgttcct acaaagaaca ggggattgac ctgatcccga
    21181 ccatccatga gggatatgaa gtccgggcaa tggagaaaaa gggaatcaaa acggtgatcg
    21241 gagaattgaa ccgggcaatc cggcagttta accagatgtt tatttctctg aaagaatcca
    21301 ttcagtggat gaagacagct tatgaggaaa tgaaagcaga gctggatcgc aggcagaacc
    21361 cgacactgct ggaaagcctg caggattatt atgataaaaa gacacagggc agaccaccac
    21421 ttcccaattt ttatgcggag atgaaacaga gaggtaagaa tctctccaat ttacaggagt
    21481 ttgcaaaatc catcagttat cttcagacac atcagataga aacgatggat gacttgcagg
    21541 aacggattga ggaactgaac ggtgttgttt ctgtcagtaa aaaagaaatt tctgaaaagc
    21601 ggaaacagtt aaaggaactt gagaatctgg aaaagatggc agaggtaata aaaacaaatc
    21661 agccattgat tgatgagtat aaccatttct attttcagaa aagacgggag agatattatc
    21721 agcagcataa aaaagaaatc aattattacc gcaaatgtga acgggaactg aaacaacatc
    21781 tggataaaaa tggaaaagtg ccaactgcca gatggaaacg ggagaaagaa gaacttcagg
    21841 cggggatcga agagctgaaa gcagataatc agccgtacca ggaggaacta acttttgtga
    21901 aaaaggtaca gagctgtgcg gatattgcca gacgtgaccg ggaaatggca gaagcagata
    21961 cttccgggcg ttcagaagaa aaaagggaga agcaggtgaa gttcccgaca ttttacacag
    22021 ttcaggcgaa agagattctt gaagaaaacg ggaaagcaga gcaacagccg ttgaaccagt
    22081 cggaacagaa tccagaaaag aagacctctc ttctgagaaa actggatgaa aaaaagaaag
    22141 agtgtgcaga gcgtgatgca aaacaacagg cagtgaagaa aaagcgaaac tatgacatga
    22201 gtttataaca gtctgtcagc aggacagacg ggaaaggatg agcaaaatgc agtatacaga
    22261 agaacaaatt ataagagcaa atcagacaga tcttgtttct tttctgagcg cacagggaga
    22321 acaactaggg aaaagcggaa aggaataccg ttggaaaaag catgacagtg taacggtatc
    22381 cggaaaccgg tggtacaggc acagtcaggg gaggggtggc tatccggtgg attttgtgat
    22441 ggaattttac aatgccacat ttccggaagc tgtcaagatg ctaaccgggg aggaaggcga
    22501 aggcaggaac agtacctgcc ccgccccgtc ccctgacttc cgattaccag agaaagagga
    22561 aaataatgat agaatcatca gatatctgac agaaaacaga gggattgaaa agaatatggt
    22621 ggaggaatgg atcggcagtg gggatattta tgaagaaaag aagcaccata atgtggtgtt
    22681 tgttggcaga gatgcagacg gtattccaag atatgcccat tgcaggggaa ctggtgaaac
    22741 aaaataccgg ggagatgtgg ctgaatctga taaatcttac ggattctgtc acagagggac
    22801 ggataatcag ttgtttgtgt ttgaagcagc cattgatctg ctttctttta tccaattatt
    22861 tcccaaagac tggaaaaagc ggagctacct gagtctgggt ggcatttcca gtgcggcact
    22921 gatggctttt ctttctgaac gccctcagat tacttcggtt ttcctgtgcc tggataatga
    22981 ccatgcaggg aatgaagcca gtgagaaatt ggcaatagaa atcccggacg gatacagtgt
    23041 catccgtctg aaaccgtccc gaaaggactg gaatgaaatc ctctgtgata aaaatgcaga
    23101 caggaagaaa tcaatcattg aaacagttac aatgaaagta ccggagaagg aagaactggt
    23161 gccgatgttg tgttatgaag acattgaaca gaccagtgta gaatggctgt ggtttcccta
    23221 tctgccattt ggaaaactga cgattattca gggcaatcca ggagagggaa aaacttattt
    23281 tgccatgatg ctgaccgcag cctgcacgaa ccgtaagact ttcccgaaca tggaagagat
    23341 tgaacctttt aatgtgatct atcagactgc agaagacggt atgggagata cgattaagcc
    23401 gaggctggtg gaagcaggag cagacctgag tcgggtgatg gtcattgatg atacagagga
    23461 agcattgacg ttatccgatg accgtattga aaaagcaatc cgtcagaatc aggtcagact
    23521 tttaatcatt gatccggtac aggcttttat cggtgccgat gtggatatga accgggcaaa
    23581 tgaagtccgt ccggtattcc ggaaactggg aatgatcgct gaaaagaccg gttgtgcgat
    23641 tgtgctgatc ggacatctga ataaatcttc agggacacag agtacttacc ggggacttgg
    23701 ttctattgat atcatggcgg cggtgagaag cctgcttttt atcgggaaag taaagaaaga
    23761 cccgacaaca agagtactga tccatgagaa aagttctctt gcacctccag gtgagacgat
    23821 ggcatttaaa cttggagatg aagaaggatt ccgctgggtg ggagcttatg agatttccgc
    23881 agatgatctt ctggacggaa aggaaggaaa gccgacagaa acaaaactgc aacggggaac
    23941 gaaactgatc tatgaactgc ttgcagacgg aaatgcagtc acaatccggg aactggatga
    24001 aaaggcaaaa gcacagggaa tctcccagag aaccatgcga gaagccagaa gccggatgaa
    24061 agaagaactg gattatcgga tgaatgaaaa gcaggaaaat accattcgat taaaaaaaca
    24121 agggagaatg ggtgacggca gaattttgga gtgaacggca ggctttatag ataccctatc
    24181 tgccgtctgc cgtctgccgg tgcaaggcaa tcaaacggaa atagcaggta tccatacaga
    24241 ataaggattg ctgtgaatct ggaacaaaaa tgaattgtaa tatggcggat ataatggtat
    24301 gattatccgg taacagcaat gttacgggga agcacctgtg acagggtgtg gctgcctcct
    24361 gatatttaca gaccggagcc tttcggggtt ctgagatgaa agaaaggagg tgcgtgatat
    24421 gacagcttat gagattatct caatattctt aggcgtactc agtttactga tcgcttttgg
    24481 tagcctgtta attgcgatta ttgcctttct tgatcggaag aacaagagga aataaaaatg
    24541 cctatcctgt ctgccaacag gataggcggt ggtcgtaaga ccatttagtc aactcaggcg
    24601 gcatccactt tggagtgggt gcttttccta taacaaatgt accacacatt cctcttgttt
    24661 tcaatgggaa aattaaaata tgcagttcat ctgacaggga tttatccctg tctttattat
    24721 atccccaatt tctgaaatgt taatacaaaa attttaatat atgtaaccaa cagtctgggc
    24781 agttaatctg taaaaatgga ttagctgctc tttttttgca aaaaatggag gttttacaat
    24841 ggcagtgaag aagaaaatta tcaacatcag agtatatttt gacggtgatc aggacgcaaa
    24901 agaagtgttt gcagaggtga tcgcacagaa aatacgtgac acgaaaacaa aagataaact
    24961 tgcaataatg aaagataagc agtataatga ttatgagttc agtaatctga aatctgcgta
    25021 ggatgtgcaa agtaacggag gtatcagatg aacaacagat atatgagtaa caatatagaa
    25081 agaaatgcaa cctggagagt tggtgtatac tgccgacttt ccaaagatga cgaattgacc
    25141 ggagagagtg ccagcatctc caaccagaga gacatactgg tgaattactg tcagtcacag
    25201 ggctggcagg tggtggatgt gtttcaggac gatggttata caggattgaa cacagaccga
    25261 cctgatttgc agagacttct caaagcctgt gaaaaaggac tggtgaatct tgtaattacc
    25321 aaagaccaga gccgactggg acgaaaccat gtacagacag gatttctgat ggaggagttt
    25381 ttcccaaagc atggagtaag gtatatcgca ttatatgata atgtggatac ctttgccgga
    25441 gacaatgaga ttgccccatt taagaacgtg ctgaacgaga tgtattccag agatatttcc
    25501 aagaaagtcc atgcgtccta tcacctgcag gcaaccaagg gaaagtttac aggtgttgtt
    25561 ccaccactgg gatatcagaa agacccggag gaaaagggac atctcctgat tgacgacgag
    25621 acagcaccga ttgtccggaa aattttccag tgggcggtgg acggtcatgg gattaacttt
    25681 atatccagag aactggaacg ccagcagatt ccctgcccgt catggtggca ccggaaacgg
    25741 ggactccgca cctattacac aaaatgggaa aagaaagacc cggaaaatgg gaaatatgta
    25801 tgggatgaat cctatctgaa atccctgctg atgaatcccg tttactgtgg tgacatggct
    25861 tcccagaaaa ggtactaccg tttcaagatt ggcaatcagg gagataaagc tccggaggac
    25921 tggatcacag tcagggatac ccacgaagca atcattccgc aggatgtatt tgatctggta
    25981 caggcaaaga tgaaaggcag gaaacgccag acagaacagg gagaatacag catgtttgcc
    26041 ggattactcc gctgtgcaga gtgtggcggt gcgttgacgt tgaagaagac gcacaccaaa
    26101 gaccagcatg aagtttatac ctgttccact tatatccata aggggaaagc acattgtacc
    26161 cagcaccggg tggacgctga tgacctgtat gatgcagtcc ttaccagaat ccaggaatgt
    26221 gcaaaagcag ttaccggaga cggaacagaa ctggaaaacc gggtaaagga gttatgcgag
    26281 gaagatacac agggacacag ggaaagtctg gagaagctgg tatcaaagca gaaagaccgc
    26341 ctggaaacgt tagaccggct gattgcaaaa ctgtatgatg accttatcaa tgacaagatt
    26401 acagaatctg tttttgataa gatgctggag aagacacaga aggagcagac agatatcaaa
    26461 aaggaacttt tccagaatga gagtgtgctg aatacggaag aaaaactgga tgcacagtcc
    26521 cagcagtgga ttgacgatat tagcgagtat gcggacatca aagaactgga tgccaatctt
    26581 ctgaaccggc tgatcagtaa aatcgttatc tcagaaccgc aggagaaaga cggaacagaa
    26641 aattactgcc gtactcctga aacggtcaca atggaaattc atttcaactt aaaaccaata
    26701 ccggagcttg gaaccatcga aagaggttct ggctcccata aataagaaaa atctatcaaa
    26761 atttaatcta ttttaatctt ttcaaggctc atttcgagca aatattttct cataaaagac
    26821 ataaaacagc ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct ggttcccctg
    26881 ctgtccgggc tgctgggctg atcctggcct ttgtccctgt ccaaaccctt aactaatcgc
    26941 ctccgcgtcc tcccggccag accgggaggg cgcgggaaag gaggtgctac ccgtgattag
    27001 tgaaatcatt gaagaatgga taaaaggcat cctcatggac ggtatcacgg gcaacctgtc
    27061 cgggctgttc gatacggtaa atgccaaagt cggagaaatc gccgccgacg tgggacgaac
    27121 cccgcaggca tggaacagcg gtattttcaa tatgctgcac agcctgtcgg aaaccgtgat
    27181 cgtccccata gccggggcca tactcgccct tgtcatgtgc tatgagctta tccagatgat
    27241 aacggaaaag aacaatatgc acgactttga cagctccatg ttcttccgct ggatattcaa
    27301 atccgcgttt gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg gcgtattcga
    27361 cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga catccattga
    27421 ttttaacacc ctgataccag accttgaaag ccaattggag gctatgagcg tgggcgctct
    27481 tttggggctg tggttccaaa cccttgttgt gggcctcacc atgaatatcc tttccatctg
    27541 cattttcctt gtgacctatg gccgcatggt ggaaatctat gttgtcacgt cgctggggcc
    27601 tatccccctt gccacaatgg gcaacgcgga atggcgcggc atgggccaag ggtatctgaa
    27661 atccctgctg gcgctgggct tccaagcctt tttaatcatg gttgtggtgg gtatctacgc
    27721 ggtgctgata cagaatatcg gttccgcttc cgacatatcc ggcgcaatct ggggctgtat
    27781 gggctatacg gtgctgctgt gcttctgcct gttcaagacc ggcagtatat caaaggccgt
    27841 attcggggcg cattgatccc aaaatccaag catacacagg aggaacttgt ttatttcaac
    27901 gtacaacatc atttacgctg acccgccgtg gcggtatgac tgcaaaaacg tacagggcgc
    27961 agcggaaaat cactattcca ccatgagcat tgacgagctg tgcgccctgc cggtggaacg
    28021 gctggcgtcc aaagactgcc tgctgttttt gtgggccaca ttccccatgc tgccggaagc
    28081 tctgcggtta atcaaagcgt ggggcttttc attcaagacc gtggcttttg tatggctgaa
    28141 acagaaccgt aaaagcctta catggtttta cgggctgggc cgctggacaa ggggaaacgc
    28201 ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct ctgtccatca
    28261 gtttattatc agcccgattg aacaacacag caaaaagccg gatgtgaccc gtgaaaaaat
    28321 cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt gccagacaga aaacccccgg
    28381 ctgggacgtg tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa aggaggtgcc
    28441 aaatggctta tgtaaccgtc cccaaggact taacccatgt gaaaagcaag gttgtgtttg
    28501 ggcttaccaa acggcaactg atctgttttt ccggggcgct ggtgatcggc gtccccttat
    28561 attttctcac ccgtgactat cttaccaaca gcgcggcggc cctgctcatg gtatttgcca
    28621 tgctgccggg ctttttgctt gccatgtacg agcgtcacgg ccagcccctt gaagtggtga
    28681 ttatgcagat catccagtgc tgcttcatcc agccaaagga gcgtccctat cagaccgaca
    28741 acacctacac cgcccttgtg cggcaatacc agatggaaca ggaggtaaag gccattgtcc
    28801 aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga cccgcgccca gcgaaaagaa
    28861 attgacgcgg taatccgtaa atataagggc gacggcaggc cccacacggc gcagcagagc
    28921 atcccctacg aggtcatgta cccggacggc gtgtgccggg tatcgcccgg cgtcttttcc
    28981 aagtgcattg agtttgcgga catcagctat cagcttgccc agccggacac gcagacggct
    29041 atctttgaaa aactgtgcga cctgtataac tatgtggacg cctccattca tatccagttt
    29101 tctttcctca atcggaaagt tgacccggtg cagtacgcca agagctttga gatcgcgccg
    29161 cagggggacg acttcgacga catccgggcc gagtacaccg gcattctgca aaagcagctt
    29221 gcaaacggca acaacggcat ggtaaagacc aaatacctga cattcaccat cgaggccgaa
    29281 agcgtgaaag cggcgcgagc cagattgaag cgcatcggct ttgacctgtt aggctatttc
    29341 aagagcatgg gggccgtagc ccatgtgatg gacggctggg aacggctgaa cctgctgcat
    29401 ggcgtgtacc acccggacgg cgagatattc aattttgact ggaagtggct ggccccgtcc
    29461 ggcctctcca ccaaggattt tattgccccg tcctccctgt gcttcggcaa tgcaaagaca
    29521 ttcggcatgg gcggcaaata cggcgcggta agtttcctgc aaatcctgtc cccggagctg
    29581 tcggacgata tgctggccga cttcctcaat acggaaagcg gtgttttagt caatctccat
    29641 gtgcaggcca ttgaacagac caaggcgatc aagaccatca agcgcaaaat taccgacctt
    29701 gacgctatga agattgcgga gcagaaaaag gccgtccgca gcggttacga tatggacatt
    29761 cttcccagcg accttgccac ctacggcgag gacgccaaga agctgctgac aaagttgcag
    29821 acccgcaatg aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc cgacaccaag
    29881 caaaagctga ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca taactgtccc
    29941 cttgtccggc tggactacca gcaggaacag ggccttgcaa gcagcctgcc gctgggcgtc
    30001 aatcagatca agatacagcg cagccttacg acttccagcg tggccgtgtt cgtccccttt
    30061 gtgacgcagg agctttttca gggcggcgcg gccatgtatt acggcatcaa cgccaagtcc
    30121 cgcaacatga ttatgctgga caggaagcag gcccgttgcc ccaatgcctt gaagctcggc
    30181 acccccggca gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt gttcttaacg
    30241 acgcctgacg atatttttat ttcagacccg gaggccgaat attaccccct tgtcaaacgg
    30301 ctccacggac aggtaatccg gctgtcgcct accagcaagg actttgtgaa cccgctggac
    30361 atcaacctga attattcgga ggacgataac ccgctggccc tgaaatccga ctttgtgctg
    30421 tcgttctgtg agcttgtcat gggcggcaag aacgggctgg aagccattga aaagaccgtg
    30481 atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg cccggagaat
    30541 atgccgatcc tgtccgacct ccacaaagcg ttgttagatc agcatgtccc ggaggctgac
    30601 cgggtggcgc aggcccttga cctgtatgtg tccggctccc tgaacgtatt taaccaccgt
    30661 accaacgtgg acatcggcaa ccggcttgtg tcctttgaca ttaaggagct gggcaagcag
    30721 ctcaaaaagc tggggatgct cattgtccaa gaccagatat gggggcgcgt cacggccaac
    30781 cgaagccaag gaaaggccac atggtatttt gccgacgagt tccatttgct tttgaaagag
    30841 gaacagacgg cggcctattc tgcggagatt tggaagcggt ttaggaaatg gggcggcatt
    30901 ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct ccccggaaat tgaaaatatc
    30961 cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt ccggggatag aaaaatcctt
    31021 gcggaacgct tgaatttgtc caccgaacag cagaagtaca tcgacaattc cgaacccggc
    31081 gagggccttt tgatctttga aaacgtggtg ctgcccttta ccaaccccat cccgcacaac
    31141 acccagcttt acaagattat gacgacccgc ttaaacgagg tggcgggagt atgacccggc
    31201 gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga aagtgacgca
    31261 ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc accggcgagg tggagaacat
    31321 ttccagccgg gaggccgaac aggatttgtc cgcgccgggg ccgggcggca ccgccgataa
    31381 gctgctggaa cgggccgacg ccatccatga cggccataag gccaagaaag ccgcgaagca
    31441 ggccggggct gtggccgccg agggggacgc tgccctgcac cggccatccg cccggctcca
    31501 actgtcggag gaagaacggg ccgacccgga tttacagccc tatatccgaa aatcggacaa
    31561 acgggccgat aagctggata aggcccgcgc cgccatcccc aagaagcgcg tcccggtcaa
    31621 ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc gtcctgcact ttgacaaaca
    31681 ggacaagggg cctccctccc tgaaacccaa tccggcaggc cgtcctgttt ccgaggtttt
    31741 tctattcggc cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg tggagggcgg
    31801 ccataagggg gaggaacttg ccgagcggca ggccggaaaa gccatccgca gcaccatccg
    31861 gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc tattgtctgc
    31921 caacgcggag tatttctacc agaaatcttt gcgggataac ccggagatcg cacaggcggc
    31981 cagcaacccc atttcccggt tctggcagaa acagcagatc aagcgccagt acgccaaggc
    32041 cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca agacagccgg
    32101 ggcctccacg aaagcggcca aggccgcagc caaagaaagt gaacgggcgg cctcctttgt
    32161 ggcccgccac tggaaagggg cgctggccgt ccttgtgttg gggcttttga tgctgcttat
    32221 catcggcggc ttacagtcct gcaccgccat gttcggcagc gccggaagcg gcatcacggc
    32281 gtcctcttac ttttccgagg atagcgatat gttggccgcc gaggacgcct acgcggagct
    32341 ggaagccgag ctgcaagact atctggacaa ctacgaggcc acccatgact atgacgaata
    32401 ccattttgac cttgacgaga tcagccatga cccctatgtg ctgatttcca ttctgtcggc
    32461 gctccatgac gggatattta ccatcgggga ggtacagggt gatttgtcca tgctctttga
    32521 caagcaatat atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca ccgaaaccga
    32581 tacatggacg gacgcggagg gcaacaccca tacagacacc taccaagtgc cgtatgacta
    32641 ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc catgtgccag tctatatcat
    32701 ggacgaggaa acgctaagtt tctattccct gtatatgtcc acgctaggaa accggcctga
    32761 tctgttttcc ggcagccagt accccaacgc ctccaccctg acgccgccga cctactacga
    32821 cgtacccccg gaggctttgg aggatgccac ctttgccgcc atgctcaaag aggcggaaaa
    32881 atatatcggt tatccctatg tgtggggcgg cagctctccc agcaccagct ttgattgcag
    32941 cggcttcatt tcgtgggtaa tcaatcatag tggatggaac gtgggacggc tgggggcgca
    33001 gggcctatat aacatctgca cccctgtttc cagttcgcag gccaagcccg gcgaccttgt
    33061 atttttcaag ggaacctacg acacccccgg cgtgtcccac gtcggcctgt atgtgggcaa
    33121 caatgtgatg ctgcattgcg ggaacccgat ttcctacacg aaccttactt caaactactg
    33181 gcaatcccat ttttacagtt acgggcgttt accttaacgc cagaaaggag ctttcaatta
    33241 tggcaaccaa acttgaccgc atcgaaaagg acatccagaa aaccaagtct aagatcgcgg
    33301 agtttcagaa acagcttcgg gagctggaaa cgcagaaaac cgaacaggag aatttgcaga
    33361 tcatccagct tgtgcgcggc atgaacatga agccggagga atttgccgcg ttcttacgca
    33421 gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca tgaacaggag gacaatgccc
    33481 atgaagaata agaccagacg gatttcccgt attctccccg cacttctggc cgtgatgctc
    33541 tgcgtcacgg cctttcccgt taccgccctt gcaggcggca atgacccggc accggccccg
    33601 ctgccggagg ctacaaccga acccgctacg ggcggtttgg agcctgaaac agacgaaacc
    33661 ggcaccgcca cggacgggac agaaaccgag gccgaaaaag ataccggccc tctgaccggc
    33721 aaggacatca ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat tgccgccacg
    33781 gacgacggca tccagatcac caccggcacc gacaaaaaag aacctgaaca gaccggcacc
    33841 gtcaccacca acggcggcag gctgaatgtc cgcaccggcg cggggcttga caagaccgcg
    33901 ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg cgactggatt
    33961 aaaattctct tgccggagcg catcggctat gtccattccg actatatgac cgtcagcgaa
    34021 aaggaagtag cggctaccgg cgagggcggt ttttccctct ccattgaccc ggaggaaatt
    34081 gcttctctgc tggaactgtt caatggcggc ggggccagcg ccgccctgtc cccggacggg
    34141 aacctgtcac tgattgacga catcggcagc gccgcgaaaa atggcaagca gtttatcacc
    34201 gtggaaacca aaaacggcaa tgtgttttat ctgattattg accgggacga cgagggaaaa
    34261 gaaactgtcc atttcttaaa tcaggtggat gaagccgatc tgctgacgct gatggaggac
    34321 ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa ggccggggct
    34381 gtgaacacga attgcccggt atgtaaaacc aacctgaccg agtgcagcgg cccggagccg
    34441 caggaacccc agccggagga acccgaagcc ccggaggaag aacccaaggg cggcgcgggc
    34501 ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct ttactatttc
    34561 aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg atttgggcga gtatgatttt
    34621 ggggaggacg aggatttgga ggacgaggaa cccgaaaccg agtttgaccc ggaagctgat
    34681 tttgtgagtg aacaggagga cgaggaatga catataaact tgtaatcgcg gaaaaaccca
    34741 gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac ggctatttgg
    34801 aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt gccgacgccg
    34861 gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt ctcccggaaa
    34921 catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc tgttcgctga
    34981 tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc gagggggaat
    35041 tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag cgcctttgga
    35101 tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa ccgggcggcg
    35161 actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg ctgatcggca
    35221 ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc
    35281 agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat ttcaagaaag
    35341 agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc agccgctttt
    35401 ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg
    35461 tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac cttaccacct
    35521 tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt gactatgccc
    35581 aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa tatcttaccg
    35641 acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg gtgccgtttg
    35701 ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg
    35761 accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc actctggccg
    35821 agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc gtggccgggc
    35881 cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag tttaccgcca
    35941 agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc cgttcttcct
    36001 taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg
    36061 gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc
    36121 cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg
    36181 aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc
    36241 tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca
    36301 tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg
    36361 aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca
    36421 tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga
    36481 aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct
    36541 atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga
    36601 atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc
    36661 tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg
    36721 acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca
    36781 ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc
    36841 ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc
    36901 tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa
    36961 gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc
    37021 acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag
    37081 gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac
    37141 atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt
    37201 gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag
    37261 aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac
    37321 gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg
    37381 ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag
    37441 gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc
    37501 gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa
    37561 gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg
    37621 ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg
    37681 attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct
    37741 tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc
    37801 atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat
    37861 gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag
    37921 cagatggaga aaatcgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc
    37981 gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc
    38041 agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg
    38101 cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt
    38161 gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc
    38221 aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc
    38281 aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc
    38341 agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg
    38401 gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc
    38461 gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc
    38521 gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc
    38581 acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg
    38641 gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta
    38701 aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca
    38761 gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag
    38821 ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc
    38881 gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta
    38941 atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta
    39001 ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg
    39061 cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg
    39121 cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg
    39181 gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag
    39241 gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct
    39301 ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa
    39361 acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac
    39421 agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat
    39481 atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt
    39541 tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc
    39601 ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct
    39661 acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag
    39721 ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag
    39781 gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag
    39841 ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc
    39901 acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag
    39961 cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag
    40021 ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt
    40081 gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc
    40141 aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg
    40201 ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag
    40261 ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg
    40321 ctggacggat gcccccatct gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg
    40381 tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctctccaa caataacgcc
    40441 gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat
    40501 gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa
    40561 atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc
    40621 cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc
    40681 gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt
    40741 gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc
    40801 gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg
    40861 cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc
    40921 gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc
    40981 gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc
    41041 cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg
    41101 gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc
    41161 actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa
    41221 accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg
    41281 gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag
    41341 gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg
    41401 gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt
    41461 gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc
    41521 aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag
    41581 gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa
    41641 ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc
    41701 aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa
    41761 aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc
    41821 gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac
    41881 aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc
    41941 ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca
    42001 gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt
    42061 cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag
    42121 tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct
    42181 taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt
    42241 gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct
    42301 cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa
    42361 atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc
    42421 tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc
    42481 cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg
    42541 tccatcctga tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga
    42601 accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc
    42661 ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata
    42721 agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct
    42781 ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg
    42841 gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct
    42901 cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg
    42961 aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc
    43021 cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat
    43081 tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat
    43141 gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta
    43201 cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta
    43261 cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa
    43321 aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc
    43381 catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc
    43441 cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat
    43501 ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt
    43561 caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga
    43621 ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt
    43681 gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc
    43741 ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa
    43801 ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc
    43861 ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc
    43921 tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac
    43981 ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg
    44041 tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt
    44101 gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac
    44161 ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc
    44221 aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag
    44281 catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt
    44341 gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat
    44401 aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc
    44461 tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg
    44521 atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag
    44581 ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt
    44641 ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc
    44701 tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa
    44761 gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact
    44821 ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta
    44881 cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag
    44941 ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg
    45001 gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg
    45061 ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct
    45121 ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg
    45181 gcagactggg aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta
    45241 ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg
    45301 cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta
    45361 cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta
    45421 tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag
    45481 gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg
    45541 atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat
    45601 ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc
    45661 ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa
    45721 gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga
    45781 taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc
    45841 gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg
    45901 tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct
    45961 ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat
    46021 accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac
    46081 atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt
    46141 ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac
    46201 cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca
    46261 aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg
    46321 ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg
    46381 ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag
    46441 gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc
    46501 atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga
    46561 aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc
    46621 gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct
    46681 ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt
    46741 ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca
    46801 tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct
    46861 gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa
    46921 gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc
    46981 gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact
    47041 ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt
    47101 gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga
    47161 ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc
    47221 ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg
    47281 gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct gctactggtt
    47341 gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa
    47401 ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg
    47461 cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat
    47521 attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt
    47581 tttagacccg actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa
    47641 aacaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt
    47701 gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg
    47761 ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg
    47821 ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc
    47881 aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag
    47941 gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct
    48001 acaagcgttc tgttggaggg aatgcttgga aatatcggtg actacaaaga tcattctaag
    48061 tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg
    48121 gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg
    48181 cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg
    48241 ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag
    48301 gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg
    48361 atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac
    48421 attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga
    48481 agcagaaaaa gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc
    48541 atgagcctcc agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta
    48601 ccgctcacat caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac
    48661 cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag
    48721 atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg
    48781 cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata
    48841 tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct
    48901 gcacgctgtt tttggggtgg agactggctt ccgtattgga ggcaacacag ataccgccca
    48961 tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc
    49021 ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc
    49081 agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga
    49141 tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag
    49201 tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg
    49261 aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag
    49321 aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca
    49381 atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct
    49441 atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca
    49501 acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag
    49561 ggggttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga
    49621 cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa
    49681 aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga
    49741 aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc acaataccgg
    49801 atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt
    49861 aaacggctta ttgataagac cgggggaaac cttttctttc tggtggctgg tacgccatgc
    49921 ggacaaagat accccctata aagacggcct tacggtgacc aatggtaaac tcaccaccat
    49981 gtcgggcggc ggtatgtgcc agatgagcaa tttactattt tggatgttcc tgcatacgcc
    50041 attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga
    50101 cgagattaaa ggggtggatg caaccattcc agagggctgg attgatttaa aagtgcgaaa
    50161 cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca
    50221 ggtgtccgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta
    50281 tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt
    50341 aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta
    50401 tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt
    50461 actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt
    50521 catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct
    50581 ggcaatcaat gcgttagcgt aggccataag tccgaggttt ccgaggcgac aattcttgcg
    50641 ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat
    50701 acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc
    50761 gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg
    50821 cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat
    50881 atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg
    50941 ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc
    51001 cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg
    51061 gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta
    51121 atcaacaccg cgcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc
    51181 ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc
    51241 gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat
    51301 gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa
    51361 aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt
    51421 cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaat ccgcaataga
    51481 aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa
    51541 cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc
    51601 catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga
    51661 aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaattcgggg aggatggtgc
    51721 gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc
    51781 cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt
    51841 tcccgaattt caaattattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc
    51901 tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac
    51961 ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga
    52021 gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat
    52081 tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc atcaggaaaa
    52141 cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga
    52201 ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg
    52261 gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa
    52321 taccatgccc ggttttacat cgtacagccg ctacccacgt atggtggccg ccgcaggaat
    52381 cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg
    52441 gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac
    52501 gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc
    52561 ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc
    52621 tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt
    52681 tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga
    52741 gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca
    52801 attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc
    52861 gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa
    52921 aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa
    52981 tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt
    53041 tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca
    53101 tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag
    53161 tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg
    53221 cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc
    53281 agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct
    53341 ctaatccact tccaccactc aaaaaagcct tgcccctc
//
LOCUS       DAJSNV010000011        51210 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSNV010000011 DAJSNV010000000
VERSION     DAJSNV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.011"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            330..1175
                     /locus_tag="ON516_000602"
     CDS             330..1175
                     /locus_tag="ON516_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3344108.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ON516_000603"
     CDS             1456..2676
                     /locus_tag="ON516_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3344109.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ON516_000604"
     CDS             2673..5393
                     /locus_tag="ON516_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3344110.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ON516_000605"
     CDS             5377..6321
                     /locus_tag="ON516_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3344111.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ON516_000606"
     CDS             complement(6390..7400)
                     /locus_tag="ON516_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3344112.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ON516_000607"
     CDS             complement(7580..7924)
                     /locus_tag="ON516_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3344113.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ON516_000608"
     CDS             complement(7917..8351)
                     /locus_tag="ON516_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3344114.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ON516_000609"
     CDS             8489..9226
                     /locus_tag="ON516_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344115.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ON516_000610"
     CDS             9219..10430
                     /locus_tag="ON516_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344116.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ON516_000611"
     CDS             10634..11416
                     /locus_tag="ON516_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3344117.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ON516_000612"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ON516_000612"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3344118.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ON516_000613"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ON516_000613"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3344119.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="ON516_000614"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="ON516_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3344120.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="ON516_000615"
     CDS             15018..16067
                     /locus_tag="ON516_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR3344121.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="ON516_000616"
     CDS             16064..16342
                     /locus_tag="ON516_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3344122.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="ON516_000617"
     CDS             16317..16787
                     /locus_tag="ON516_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3344123.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="ON516_000618"
     CDS             16804..17106
                     /locus_tag="ON516_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344124.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="ON516_000619"
     CDS             17123..17494
                     /locus_tag="ON516_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3344125.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="ON516_000620"
     CDS             17466..17825
                     /locus_tag="ON516_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344126.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ON516_000621"
     CDS             17997..19004
                     /locus_tag="ON516_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3344127.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ON516_000622"
     CDS             19029..20213
                     /locus_tag="ON516_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3344128.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="ON516_000623"
     CDS             complement(20650..22233)
                     /locus_tag="ON516_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3344129.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="ON516_000624"
     CDS             complement(22811..22954)
                     /locus_tag="ON516_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3344130.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="ON516_000625"
     CDS             complement(23177..23656)
                     /locus_tag="ON516_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3344131.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="ON516_000626"
     CDS             complement(23729..24013)
                     /locus_tag="ON516_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344132.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="ON516_000627"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON516_000627"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="ON516_000628"
     CDS             complement(24357..25643)
                     /locus_tag="ON516_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3344133.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="ON516_000629"
     CDS             25781..26452
                     /locus_tag="ON516_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3344134.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="ON516_000630"
     CDS             26418..26906
                     /locus_tag="ON516_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3344135.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="ON516_000631"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="ON516_000631"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3344136.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="ON516_000632"
     CDS             27867..28631
                     /locus_tag="ON516_000632"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3344137.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="ON516_000633"
     CDS             28864..31077
                     /locus_tag="ON516_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3344138.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="ON516_000634"
     CDS             31088..31534
                     /locus_tag="ON516_000634"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3344139.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="ON516_000635"
     CDS             31705..32964
                     /locus_tag="ON516_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3344140.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="ON516_000636"
     CDS             complement(33053..34000)
                     /locus_tag="ON516_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3344141.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="ON516_000637"
     CDS             34394..35686
                     /locus_tag="ON516_000637"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3344142.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="ON516_000638"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="ON516_000638"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3344143.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="ON516_000639"
     CDS             37579..38454
                     /locus_tag="ON516_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3344144.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="ON516_000640"
     CDS             38720..40603
                     /locus_tag="ON516_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3344145.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="ON516_000641"
     CDS             complement(40678..40962)
                     /locus_tag="ON516_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3344146.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="ON516_000642"
     CDS             complement(40952..41686)
                     /locus_tag="ON516_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3344147.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="ON516_000643"
     CDS             41815..42438
                     /locus_tag="ON516_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3344148.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="ON516_000644"
     CDS             42630..43751
                     /locus_tag="ON516_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3344149.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="ON516_000645"
     CDS             43758..46886
                     /locus_tag="ON516_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3344150.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="ON516_000646"
     CDS             46905..50099
                     /locus_tag="ON516_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3344151.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="ON516_000647"
     CDS             complement(50245..50952)
                     /locus_tag="ON516_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3344152.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16758 a         8948 c        10638 g        14866 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag
//
LOCUS       DAJSNV010000012        49643 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSNV010000012 DAJSNV010000000
VERSION     DAJSNV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.013"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            292..1431
                     /locus_tag="ON516_000648"
     CDS             292..1431
                     /locus_tag="ON516_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3344153.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ON516_000649"
     CDS             complement(1498..2073)
                     /locus_tag="ON516_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3344154.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ON516_000650"
     CDS             complement(2078..3052)
                     /locus_tag="ON516_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3344155.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ON516_000651"
     CDS             3335..4303
                     /locus_tag="ON516_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3344156.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ON516_000652"
     CDS             4416..4694
                     /locus_tag="ON516_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3344157.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ON516_000653"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ON516_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3344158.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ON516_000654"
     CDS             5151..5906
                     /locus_tag="ON516_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3344159.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ON516_000655"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ON516_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3344160.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ON516_000656"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ON516_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3344161.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ON516_000657"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ON516_000657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3344162.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ON516_000658"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ON516_000658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3344163.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ON516_000659"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ON516_000659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3344164.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ON516_000660"
     CDS             10643..11185
                     /locus_tag="ON516_000660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3344165.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="ON516_000661"
     CDS             11208..12764
                     /locus_tag="ON516_000661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3344166.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ON516_000662"
     CDS             12778..13680
                     /locus_tag="ON516_000662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3344167.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ON516_000663"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ON516_000663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3344168.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ON516_000664"
     CDS             15123..15545
                     /locus_tag="ON516_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3344169.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="ON516_000665"
     CDS             16014..16244
                     /locus_tag="ON516_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3344170.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="ON516_000666"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="ON516_000666"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3344171.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="ON516_000667"
     CDS             17655..17831
                     /locus_tag="ON516_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3344172.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="ON516_000668"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="ON516_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3344173.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="ON516_000669"
     CDS             18326..18793
                     /locus_tag="ON516_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344174.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="ON516_000670"
     CDS             18878..19762
                     /locus_tag="ON516_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3344175.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="ON516_000671"
     CDS             19876..20112
                     /locus_tag="ON516_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3344176.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="ON516_000672"
     tRNA            20200..20280
                     /locus_tag="ON516_000672"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="ON516_000673"
     tRNA            20287..20358
                     /locus_tag="ON516_000673"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20483..20653
                     /locus_tag="ON516_000674"
     CDS             20483..20653
                     /locus_tag="ON516_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344177.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            20873..21580
                     /locus_tag="ON516_000675"
     CDS             20873..21580
                     /locus_tag="ON516_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3344178.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="ON516_000676"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="ON516_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="ON516_000677"
     CDS             22400..22687
                     /locus_tag="ON516_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344179.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="ON516_000678"
     CDS             22795..23115
                     /locus_tag="ON516_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3344180.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="ON516_000679"
     CDS             23196..24509
                     /locus_tag="ON516_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3344181.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="ON516_000680"
     CDS             24506..25225
                     /locus_tag="ON516_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3344182.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="ON516_000681"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="ON516_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3344183.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="ON516_000682"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="ON516_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3344184.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="ON516_000683"
     CDS             join(28786..28857,28859..29887)
                     /gene="prfB"
                     /locus_tag="ON516_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3344185.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="ON516_000684"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="ON516_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3344186.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="ON516_000685"
     CDS             30662..31555
                     /locus_tag="ON516_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344187.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..33155
                     /gene="cls"
                     /locus_tag="ON516_000686"
     CDS             31707..33155
                     /gene="cls"
                     /locus_tag="ON516_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3344188.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            33492..34349
                     /locus_tag="ON516_000687"
     CDS             33492..34349
                     /locus_tag="ON516_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3344189.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            34464..35384
                     /gene="pstC"
                     /locus_tag="ON516_000688"
     CDS             34464..35384
                     /gene="pstC"
                     /locus_tag="ON516_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3344190.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            35384..36268
                     /gene="pstA"
                     /locus_tag="ON516_000689"
     CDS             35384..36268
                     /gene="pstA"
                     /locus_tag="ON516_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3344191.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            36283..37089
                     /gene="pstB"
                     /locus_tag="ON516_000690"
     CDS             36283..37089
                     /gene="pstB"
                     /locus_tag="ON516_000690"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3344192.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            37105..37863
                     /locus_tag="ON516_000691"
     CDS             37105..37863
                     /locus_tag="ON516_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3344193.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            37876..38553
                     /gene="phoU"
                     /locus_tag="ON516_000692"
     CDS             37876..38553
                     /gene="phoU"
                     /locus_tag="ON516_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3344194.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            38804..39822
                     /locus_tag="ON516_000693"
                     /pseudo
     CDS             38804..39822
                     /locus_tag="ON516_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            39819..40586
                     /locus_tag="ON516_000694"
     CDS             39819..40586
                     /locus_tag="ON516_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3344195.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            40686..42254
                     /gene="liaX"
                     /locus_tag="ON516_000695"
     CDS             40686..42254
                     /gene="liaX"
                     /locus_tag="ON516_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3344196.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            42551..42871
                     /locus_tag="ON516_000696"
     CDS             42551..42871
                     /locus_tag="ON516_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3344197.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            42871..43221
                     /locus_tag="ON516_000697"
     CDS             42871..43221
                     /locus_tag="ON516_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3344198.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            43388..44326
                     /locus_tag="ON516_000698"
     CDS             43388..44326
                     /locus_tag="ON516_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3344199.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            44341..45171
                     /locus_tag="ON516_000699"
     CDS             44341..45171
                     /locus_tag="ON516_000699"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3344200.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            45215..46240
                     /locus_tag="ON516_000700"
     CDS             45215..46240
                     /locus_tag="ON516_000700"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3344201.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            46255..47193
                     /gene="galU"
                     /locus_tag="ON516_000701"
     CDS             46255..47193
                     /gene="galU"
                     /locus_tag="ON516_000701"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3344202.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            47318..47758
                     /locus_tag="ON516_000702"
     CDS             47318..47758
                     /locus_tag="ON516_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3344203.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            47761..48363
                     /locus_tag="ON516_000703"
     CDS             47761..48363
                     /locus_tag="ON516_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3344204.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(48422..49528)
                     /locus_tag="ON516_000704"
     CDS             complement(48422..49528)
                     /locus_tag="ON516_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3344205.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT        16377 a         8648 c        10755 g        13863 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattac
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag
    32641 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    32701 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    32761 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    32821 tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg
    32881 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    32941 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    33001 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    33061 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    33121 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    33181 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    33241 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    33301 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    33361 agaaactttt aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg
    33421 attttgattc gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa
    33481 ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta gccattcttt
    33541 tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg
    33601 ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat
    33661 tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag
    33721 tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat
    33781 tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg
    33841 taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga
    33901 gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa
    33961 cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac
    34021 aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg
    34081 ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga
    34141 cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa
    34201 gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac
    34261 aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg
    34321 attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa
    34381 cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa
    34441 acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa
    34501 aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg
    34561 tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc acctttttta
    34621 ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg
    34681 gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga
    34741 cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg
    34801 agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta
    34861 ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta
    34921 tttttggcgg gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct
    34981 tgccgactgt cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg
    35041 aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag
    35101 cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag
    35161 ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc
    35221 ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag
    35281 ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta
    35341 atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc
    35401 agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc
    35461 agctttacta ctttatatct tagttcgggg aataccgcat atttcatggt ccttcttgac
    35521 cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat
    35581 ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta
    35641 tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat
    35701 cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca
    35761 attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc
    35821 tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc
    35881 aggattggcc ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc
    35941 gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc
    36001 tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc
    36061 attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat
    36121 ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc
    36181 tgccgtgttg atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg
    36241 attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg
    36301 aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat
    36361 ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa
    36421 aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg
    36481 ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa
    36541 ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg
    36601 gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg
    36661 aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa
    36721 caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct
    36781 ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg
    36841 ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca
    36901 cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca
    36961 gctcgtatca gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa
    37021 actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat
    37081 tttggttaag acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat
    37141 ctgtattatg gaaagaaaga agcactaaaa gggatcaatc taagtttcaa tcaaggggaa
    37201 atcactgcga tgatcggtcc ttctggttgc gggaaatcca cttatctgcg ttctttgaac
    37261 cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa
    37321 gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc
    37381 cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta
    37441 aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg
    37501 tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg
    37561 cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg
    37621 gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta
    37681 gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt
    37741 atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa cgatacgaag
    37801 aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga
    37861 taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat
    37921 cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac
    37981 atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac
    38041 atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca
    38101 actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc
    38161 gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg
    38221 gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc
    38281 ctagtagctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa
    38341 agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat
    38401 cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc
    38461 ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact
    38521 gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt
    38581 taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag
    38641 aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga
    38701 tgcaagcttt tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc
    38761 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaaattagca tcatcactat
    38821 attttttatt tgtgtgctta cttcatgggt tattcagcat caagctgctt tatatgaagt
    38881 acctgtcatc gagatcgaaa aagtacgaat aaaagaggat catcaattga ttacaggaag
    38941 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
    39001 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
    39061 tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt
    39121 cattggcgcc atgttgctta ttggcggaaa acagggtcta tttgctttag gaagtctcat
    39181 catcaatatc gttttacttt acttttgttg ctcatcttta ctaaaacaga agggatttct
    39241 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
    39301 aatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
    39361 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
    39421 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
    39481 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
    39541 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
    39601 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
    39661 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
    39721 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
    39781 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
    39841 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
    39901 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
    39961 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
    40021 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
    40081 tttgtttcct actattgctt cctatcactg attttttagc gatccaaggt tttacatcgg
    40141 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
    40201 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgctagt gtagcaattt
    40261 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
    40321 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
    40381 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
    40441 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
    40501 tgtctgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
    40561 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
    40621 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
    40681 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
    40741 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaataaaaa
    40801 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
    40861 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
    40921 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
    40981 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
    41041 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
    41101 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
    41161 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
    41221 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
    41281 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
    41341 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
    41401 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
    41461 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
    41521 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
    41581 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
    41641 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
    41701 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
    41761 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
    41821 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
    41881 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
    41941 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
    42001 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
    42061 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
    42121 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
    42181 cagatggtga aatcacgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
    42241 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
    42301 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag gctatctctt
    42361 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
    42421 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
    42481 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
    42541 gaggtgagac atgatgaaaa aaaagttgac aaaatccaat aaaaatgttg ttttgacagg
    42601 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
    42661 tgtattcctg agcttagttg cttttggaag tccaatcatt ttatacattt tattagcttt
    42721 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
    42781 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
    42841 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
    42901 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
    42961 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
    43021 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
    43081 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
    43141 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
    43201 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
    43261 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
    43321 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
    43381 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
    43441 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
    43501 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
    43561 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
    43621 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cggctccaga
    43681 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
    43741 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
    43801 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
    43861 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggtcatcgat tgatcgcaga
    43921 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
    43981 cctggaacat ctaattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
    44041 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
    44101 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
    44161 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
    44221 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
    44281 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
    44341 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
    44401 gcattgatca tcgtatcagg tatcattatc gccatgtggc ttagcagcag agaggctgtt
    44461 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
    44521 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
    44581 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
    44641 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
    44701 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
    44761 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
    44821 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
    44881 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
    44941 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
    45001 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
    45061 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
    45121 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
    45181 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
    45241 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
    45301 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
    45361 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
    45421 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
    45481 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
    45541 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
    45601 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag cgaaacatga
    45661 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
    45721 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
    45781 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
    45841 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
    45901 agcaatgggt gccaacccgc tgacctttat cggattaagc ggagtaggcg atctagtcgt
    45961 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
    46021 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
    46081 agcagcgatg gaactagccc aaataatgga aatcgaaatg cccatcactc aaacaatcta
    46141 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
    46201 tgggaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
    46261 gtcaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
    46321 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
    46381 gaagcgttga aatcaggaat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
    46441 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
    46501 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
    46561 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
    46621 ccgtttgtcg ttatgctagg cgatgatatc atggaagatg aagtaccatt gacacagcaa
    46681 ttaatcgatg attacgaacg tactcatgct tctacgatcg ccgttatgca agttcctcat
    46741 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
    46801 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
    46861 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
    46921 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
    46981 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
    47041 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtatatc
    47101 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
    47161 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
    47221 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
    47281 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
    47341 attgattgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
    47401 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
    47461 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttgc tggtcaaaag
    47521 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
    47581 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
    47641 gaaagtcggc ggaatcggta aagcgacagc taaagcgacg gttgccggaa aagtcggtgg
    47701 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
    47761 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggaacagc cgcagcaatc
    47821 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
    47881 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
    47941 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
    48001 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
    48061 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
    48121 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
    48181 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
    48241 atgtcagaag caaaaccagt tgcagatgac acaaaagaaa tgatgaaaat ggcaaaagaa
    48301 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
    48361 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
    48421 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
    48481 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
    48541 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
    48601 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
    48661 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    48721 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtat attttttctt
    48781 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    48841 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgtg tttcctggtg
    48901 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    48961 aaacaccttg ttttttcaat tggtattcga tttctgcaat gatcgcttgt tcagtcacgc
    49021 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    49081 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    49141 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    49201 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    49261 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    49321 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    49381 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    49441 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    49501 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    49561 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    49621 aattttcatg aaaacagaga taa
//
LOCUS       DAJSNV010000013        44873 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSNV010000013 DAJSNV010000000
VERSION     DAJSNV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.014"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            212..604
                     /locus_tag="ON516_000705"
     CDS             212..604
                     /locus_tag="ON516_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3344206.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ON516_000706"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ON516_000706"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3344207.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="ON516_000707"
     CDS             3165..4775
                     /locus_tag="ON516_000707"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3344208.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="ON516_000708"
     CDS             5067..5936
                     /locus_tag="ON516_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3344209.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ON516_000709"
     CDS             6101..7372
                     /locus_tag="ON516_000709"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3344210.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ON516_000710"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ON516_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3344211.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ON516_000711"
     CDS             8763..9026
                     /locus_tag="ON516_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3344212.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ON516_000712"
     CDS             9471..9920
                     /locus_tag="ON516_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3344213.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ON516_000713"
     CDS             9977..11395
                     /locus_tag="ON516_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3344214.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ON516_000714"
     CDS             11395..12255
                     /locus_tag="ON516_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3344215.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ON516_000715"
     CDS             12429..13202
                     /locus_tag="ON516_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3344216.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ON516_000716"
     CDS             13349..13714
                     /locus_tag="ON516_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3344217.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ON516_000717"
     CDS             complement(13862..15070)
                     /locus_tag="ON516_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3344218.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ON516_000718"
     CDS             complement(15089..15367)
                     /locus_tag="ON516_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344219.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="ON516_000719"
     CDS             15521..16216
                     /locus_tag="ON516_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3344220.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="ON516_000720"
     CDS             complement(16658..17455)
                     /locus_tag="ON516_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3344221.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ON516_000721"
     CDS             complement(17452..18636)
                     /locus_tag="ON516_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3344222.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ON516_000722"
     CDS             complement(18661..19908)
                     /locus_tag="ON516_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3344223.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ON516_000723"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ON516_000723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3344224.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ON516_000724"
     CDS             21888..22202
                     /locus_tag="ON516_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3344225.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ON516_000725"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ON516_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3344226.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="ON516_000726"
     CDS             22887..23531
                     /locus_tag="ON516_000726"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3344227.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ON516_000727"
     CDS             23546..23875
                     /locus_tag="ON516_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3344228.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ON516_000728"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ON516_000728"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3344229.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ON516_000729"
     CDS             24863..25687
                     /locus_tag="ON516_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3344230.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ON516_000730"
     CDS             25680..26027
                     /locus_tag="ON516_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3344231.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ON516_000731"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ON516_000731"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3344232.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ON516_000732"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ON516_000732"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3344233.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON516_000733"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON516_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3344234.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON516_000734"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON516_000734"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3344235.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="ON516_000735"
     CDS             31657..32451
                     /locus_tag="ON516_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344236.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ON516_000736"
     CDS             32441..34483
                     /locus_tag="ON516_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344237.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ON516_000737"
     CDS             34764..34913
                     /locus_tag="ON516_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3344238.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ON516_000738"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ON516_000738"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3344239.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ON516_000739"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ON516_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3344240.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ON516_000740"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ON516_000740"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3344241.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ON516_000741"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ON516_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3344242.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ON516_000742"
     CDS             39330..40412
                     /locus_tag="ON516_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3344243.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ON516_000743"
     CDS             40412..41215
                     /locus_tag="ON516_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3344244.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ON516_000744"
     CDS             41285..42025
                     /locus_tag="ON516_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3344245.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ON516_000745"
     CDS             42036..43040
                     /locus_tag="ON516_000745"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3344246.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="ON516_000746"
     CDS             complement(43085..44782)
                     /locus_tag="ON516_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344247.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14424 a         7831 c         9343 g        13275 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cac
//
LOCUS       DAJSNV010000014        42890 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSNV010000014 DAJSNV010000000
VERSION     DAJSNV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.015"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..1377
                     /locus_tag="ON516_000747"
     CDS             37..1377
                     /locus_tag="ON516_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3344248.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1595..2038
                     /locus_tag="ON516_000748"
     CDS             1595..2038
                     /locus_tag="ON516_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3344249.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="ON516_000749"
     CDS             complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="ON516_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3344250.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4363..5586)
                     /locus_tag="ON516_000750"
     CDS             complement(4363..5586)
                     /locus_tag="ON516_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3344251.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5617..6456)
                     /locus_tag="ON516_000751"
     CDS             complement(5617..6456)
                     /locus_tag="ON516_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3344252.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6473..7153)
                     /locus_tag="ON516_000752"
     CDS             complement(6473..7153)
                     /locus_tag="ON516_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344253.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7155..8228)
                     /locus_tag="ON516_000753"
     CDS             complement(7155..8228)
                     /locus_tag="ON516_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3344254.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8284..8522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8565..8798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8875..9546)
                     /locus_tag="ON516_000754"
     CDS             complement(8875..9546)
                     /locus_tag="ON516_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3344255.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="ON516_000755"
     CDS             complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="ON516_000755"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3344256.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12970..13407
                     /gene="ndk"
                     /locus_tag="ON516_000756"
     CDS             12970..13407
                     /gene="ndk"
                     /locus_tag="ON516_000756"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3344257.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13429..14295)
                     /locus_tag="ON516_000757"
     CDS             complement(13429..14295)
                     /locus_tag="ON516_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3344258.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14455..16548)
                     /locus_tag="ON516_000758"
     CDS             complement(14455..16548)
                     /locus_tag="ON516_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3344259.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17297..17920)
                     /locus_tag="ON516_000759"
     CDS             complement(17297..17920)
                     /locus_tag="ON516_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344260.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18079..19476)
                     /locus_tag="ON516_000760"
     CDS             complement(18079..19476)
                     /locus_tag="ON516_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3344261.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19508..20479)
                     /locus_tag="ON516_000761"
     CDS             complement(19508..20479)
                     /locus_tag="ON516_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3344262.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20853..21278
                     /locus_tag="ON516_000762"
     CDS             20853..21278
                     /locus_tag="ON516_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344263.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21766..22467)
                     /locus_tag="ON516_000763"
     CDS             complement(21766..22467)
                     /locus_tag="ON516_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3344264.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22483..22923)
                     /locus_tag="ON516_000764"
     CDS             complement(22483..22923)
                     /locus_tag="ON516_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3344265.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22923..23498)
                     /locus_tag="ON516_000765"
     CDS             complement(22923..23498)
                     /locus_tag="ON516_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3344266.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24346..24825)
                     /locus_tag="ON516_000766"
                     /pseudo
     CDS             complement(24346..24825)
                     /locus_tag="ON516_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24984..25685)
                     /locus_tag="ON516_000767"
     CDS             complement(24984..25685)
                     /locus_tag="ON516_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3344267.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25793..27202)
                     /locus_tag="ON516_000768"
     CDS             complement(25793..27202)
                     /locus_tag="ON516_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3344268.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27250..29115)
                     /locus_tag="ON516_000769"
     CDS             complement(27250..29115)
                     /locus_tag="ON516_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3344269.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29236..30087)
                     /locus_tag="ON516_000770"
     CDS             complement(29236..30087)
                     /locus_tag="ON516_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3344270.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30397..31110)
                     /locus_tag="ON516_000771"
     CDS             complement(30397..31110)
                     /locus_tag="ON516_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3344271.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31178..31504)
                     /locus_tag="ON516_000772"
     CDS             complement(31178..31504)
                     /locus_tag="ON516_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3344272.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31736..32953)
                     /locus_tag="ON516_000773"
     CDS             complement(31736..32953)
                     /locus_tag="ON516_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3344273.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(33035..33238)
                     /locus_tag="ON516_000774"
     CDS             complement(33035..33238)
                     /locus_tag="ON516_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3344274.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(33699..33929)
                     /locus_tag="ON516_000775"
     CDS             complement(33699..33929)
                     /locus_tag="ON516_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344275.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(33926..34348)
                     /locus_tag="ON516_000776"
     CDS             complement(33926..34348)
                     /locus_tag="ON516_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3344276.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            34853..35206
                     /locus_tag="ON516_000777"
     CDS             34853..35206
                     /locus_tag="ON516_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344277.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            complement(35266..35433)
                     /locus_tag="ON516_000778"
     CDS             complement(35266..35433)
                     /locus_tag="ON516_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3344278.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(35552..37471)
                     /gene="tet(M)"
                     /locus_tag="ON516_000779"
     CDS             complement(35552..37471)
                     /gene="tet(M)"
                     /locus_tag="ON516_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3344279.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(37487..37603)
                     /locus_tag="ON516_000780"
     CDS             complement(37487..37603)
                     /locus_tag="ON516_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3344280.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(37848..38780)
                     /locus_tag="ON516_000781"
     CDS             complement(37848..38780)
                     /locus_tag="ON516_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3344281.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(38777..39778)
                     /locus_tag="ON516_000782"
     CDS             complement(38777..39778)
                     /locus_tag="ON516_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3344282.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(39775..41952)
                     /locus_tag="ON516_000783"
     CDS             complement(39775..41952)
                     /locus_tag="ON516_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3344283.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(41955..42872)
                     /locus_tag="ON516_000784"
     CDS             complement(41955..42872)
                     /locus_tag="ON516_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3344284.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
BASE COUNT        12271 a         8979 c         7367 g        14273 t
ORIGIN      
        1 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
       61 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
      121 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
      181 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
      241 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
      301 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
      361 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
      421 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
      481 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
      541 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
      601 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
      661 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
      721 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
      781 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
      841 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
      901 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
      961 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     1021 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     1081 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     1141 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     1201 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     1261 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     1321 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     1381 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     1441 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     1501 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     1561 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     1621 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     1681 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     1741 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     1801 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     1861 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     1921 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     1981 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     2041 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     2101 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
     2161 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
     2221 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
     2281 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
     2341 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
     2401 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
     2461 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
     2521 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
     2581 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
     2641 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
     2701 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
     2761 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
     2821 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
     2881 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
     2941 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
     3001 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
     3061 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
     3121 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
     3181 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
     3241 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
     3301 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
     3361 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
     3421 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
     3481 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
     3541 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
     3601 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
     3661 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
     3721 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
     3781 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
     3841 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
     3901 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
     3961 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
     4021 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
     4081 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
     4141 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
     4201 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
     4261 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
     4321 gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc
     4381 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
     4441 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
     4501 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
     4561 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
     4621 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
     4681 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
     4741 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
     4801 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
     4861 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
     4921 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
     4981 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
     5041 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg
     5101 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
     5161 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
     5221 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
     5281 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
     5341 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
     5401 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
     5461 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
     5521 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
     5581 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
     5641 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
     5701 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
     5761 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
     5821 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
     5881 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
     5941 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
     6001 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
     6061 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
     6121 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
     6181 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
     6241 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
     6301 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
     6361 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
     6421 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
     6481 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
     6541 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
     6601 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
     6661 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
     6721 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
     6781 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
     6841 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
     6901 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
     6961 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
     7021 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
     7081 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
     7141 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
     7201 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
     7261 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
     7321 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
     7381 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
     7441 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
     7501 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
     7561 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
     7621 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
     7681 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
     7741 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
     7801 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
     7861 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
     7921 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
     7981 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
     8041 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
     8101 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
     8161 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
     8221 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
     8281 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
     8341 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
     8401 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
     8461 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
     8521 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
     8581 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
     8641 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
     8701 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
     8761 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
     8821 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
     8881 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
     8941 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
     9001 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
     9061 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
     9121 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
     9181 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
     9241 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
     9301 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
     9361 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc
     9421 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
     9481 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
     9541 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
     9601 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
     9661 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
     9721 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaacct tgtaaaagga
     9781 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
     9841 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
     9901 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
     9961 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    10021 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    10081 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    10141 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    10201 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    10261 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    10321 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    10381 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    10441 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    10501 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    10561 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    10621 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    10681 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    10741 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    10801 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    10861 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    10921 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt
    10981 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    11041 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    11101 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    11161 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    11221 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    11281 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    11341 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    11401 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    11461 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    11521 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    11581 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    11641 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    11701 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    11761 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    11821 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    11881 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    11941 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    12001 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    12061 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    12121 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    12181 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    12241 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    12301 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    12361 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    12421 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    12481 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    12541 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    12601 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    12661 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    12721 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    12781 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    12841 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    12901 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    12961 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    13021 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    13081 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    13141 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    13201 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    13261 ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct
    13321 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    13381 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    13441 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    13501 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    13561 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    13621 atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    13681 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    13741 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    13801 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    13861 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    13921 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    13981 tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc
    14041 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    14101 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    14161 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    14221 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    14281 ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    14341 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    14401 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    14461 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    14521 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    14581 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    14641 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    14701 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    14761 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    14821 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    14881 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    14941 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    15001 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    15061 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    15121 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    15181 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    15241 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    15301 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    15361 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    15421 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    15481 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    15541 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    15601 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    15661 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    15721 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    15781 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    15841 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    15901 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    15961 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    16021 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    16081 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    16141 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    16201 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    16261 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    16321 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    16381 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    16441 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    16501 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    16561 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    16621 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    16681 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    16741 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    16801 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    16861 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    16921 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    16981 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    17041 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    17101 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    17161 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    17221 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    17281 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    17341 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    17401 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    17461 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    17521 aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca
    17581 aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa
    17641 gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca
    17701 attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt
    17761 cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag
    17821 caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac
    17881 cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt
    17941 aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag
    18001 atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt
    18061 gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca
    18121 atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc
    18181 atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata
    18241 gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac
    18301 aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt
    18361 ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac
    18421 attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat
    18481 tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt
    18541 atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag
    18601 atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc
    18661 aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag
    18721 gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga
    18781 ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc
    18841 catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg
    18901 gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc
    18961 gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc
    19021 aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc
    19081 tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc
    19141 gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt
    19201 tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc
    19261 agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc
    19321 aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt
    19381 ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc
    19441 ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta
    19501 gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    19561 cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg
    19621 ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat
    19681 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    19741 caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    19801 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga
    19861 tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt
    19921 ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga
    19981 tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt
    20041 ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc
    20101 actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa
    20161 ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    20221 tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc
    20281 cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt
    20341 tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca
    20401 gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca
    20461 catcacggat cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt
    20521 gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat
    20581 aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt
    20641 gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc
    20701 tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt
    20761 gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata
    20821 atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt
    20881 tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct
    20941 cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg
    21001 tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag
    21061 ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat
    21121 ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac gaatatatta
    21181 tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc
    21241 gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt
    21301 tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa
    21361 tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa
    21421 tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca
    21481 tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca
    21541 aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt
    21601 gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa
    21661 agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct
    21721 acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc ctaattcttg
    21781 accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt ccaattcagc
    21841 taatttttct tggatctgac ggatcacctt ttttgcatct tctggatttt ctacaaaatc
    21901 gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat accattgatt
    21961 atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt ctagctgttc
    22021 atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg
    22081 gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta gcatcgtatc
    22141 taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcgtttag
    22201 atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat cgttcactaa
    22261 agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata aaaaagcgta
    22321 ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat tttcataaaa
    22381 tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat
    22441 cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac
    22501 gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt ctgattttaa
    22561 atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacaat
    22621 gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat
    22681 tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca gttcttccat
    22741 atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg
    22801 gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat
    22861 acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact
    22921 cattattttg cctccttcaa taataactga tttttaatag atactgctcg taattcattg
    22981 accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt atccataaaa
    23041 tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc
    23101 aatattccga ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag aagttgtgag
    23161 aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc cttttcaaat
    23221 aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac atcttttcca
    23281 ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc gttattttcc
    23341 agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg
    23401 atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta actttcttta
    23461 ttaccaaaac gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa
    23521 aagtctatct tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt
    23581 tagtggtata taggatagac gccactatat atagtattta gaaaaaaatc atttttcagc
    23641 cattttatag ataaaacaaa aataaataac gtgttttcat cgatatgaaa tgacttactt
    23701 atagaagata ctgggatttc gtcctatatg ccaataacgt gtattatttt aaacaacaaa
    23761 tacttgtttc ttttattaac gaaaaaatca ttttgttctc gtgttatgtt tcactgataa
    23821 atgatgtttc tctaaataaa aaagccgacg aactaaacga tcaaaatttg atcatttttg
    23881 ttcatcagct tctcctaaaa atctttttgt taacgataaa tagctggatt tgtaaaaata
    23941 tcacttgaat cgtcactgct ggaagaaaat tcagtgataa ccgcacctgt tgcgcctgcc
    24001 tggaactagt gtttgatatt gggctcgaac gtgtactgat ctctagcatg aagaataatt
    24061 tcttttttta ctgtataata actttctttc ctttttggaa tagtacagga aagttttttt
    24121 gtttcctttc cttcgacata aggtatacca gaccctttcg acaacgaaag gttctcattt
    24181 ttccttcatc gtcatttcaa ggagggtgcc gatgctctgg acagtttcga tcgctcctaa
    24241 accaaaatct gtatattcga ttctttctaa ctctgattct ttcataccat tcctgtctca
    24301 aggtacatct attgtacttt tttcttgtaa tccattctgc ctctcctaac taattgtcaa
    24361 atcatcccaa gatatgtctt cttcctcttt ttcttcttgg atgatttctg cattcggatc
    24421 tactggtttt ttcaataata aaatggttat tcctgtgaca aaagaaccag ctaacaatgc
    24481 accaatgaat gcccatggtc tggtcatcgt agcgactgca aacattcccg atgccggaat
    24541 acggctatct gcgccccaaa acatgcttaa tccgccacct actgtcccac cgactcccgt
    24601 tgctatgaca ttgcgtgcca agtcgttcaa tacgataggg aaacatccct ctgtgatctg
    24661 acagatcccc ataggaaaag cggtcttcaa tgtatcgatt tcttgacgat tgagtatatt
    24721 tttacgaatg attttcccta agaaataagc gatcgtcaag ccgaatgggg ttaccatcga
    24781 agctaaaatc aatacagtga ttggttcata gattccttct gaaaatcgtg ccacaccccc
    24841 ttatccagaa taaatggtaa tccagaaaca gccgtgtatc tgcggtaata agcctaacca
    24901 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcagggcagc
    24961 tttttcacaa cctctttttt tatttatgtt tcataatcaa tatggtaagg atgcagctcc
    25021 gtatggtaga ccgcctccga atattcaatc atttccccaa tttgcgtaga cgatttccgt
    25081 atccgtttga tgccgatctg tgcgtctgtt ttcagaattc cttgttcttc ttctgttaaa
    25141 aatactgccg agaaatcatc agaaaatgag tcaatagtat atcctttttt cgtcagcaaa
    25201 cgatacaaag attctttgcc aaattcttct ctgctgaatt gcatcatttc ttgtggaaga
    25261 taatgaacaa aataaatata aggctgattg ttcaaataat ataagcgctt aaaacaaaca
    25321 gcttgctcac cgaaaaagga aaacaaagga tcttccttct ttatatggac caattgcaaa
    25381 tccagcgttt ctttccggac ttcatatcgg gagttctcca agatttgcgt aaagcttgcc
    25441 gctttggata atcgattgta aggacgattg cttagaaccg tcgtcccacg accacttttc
    25501 tttgcgacat attcatctga tgccagcagt tcgattgcct ttcggacagt gattttgctg
    25561 acttgaaaca gttcttctaa ttcactttct gtcggcaaca tcgaaccgac tggatatttt
    25621 cctgagaaaa tatcttcttt aattttatct gcgacttcca agtatagtac acgtgtcctt
    25681 ttcataacgt caccctttgc tttactatta ctaaaagaat agcacataaa aaataaaaaa
    25741 agaacacaaa tcacatgtga gaagtgtgtt ctttttcatt cagctgcttt aattacaagt
    25801 ttacgccatt tgaggcaatt acttctttat accaatcaaa agattttttc tttgatcgat
    25861 tcagtgtccc atttccttca tcgtctagat ccacatagat aaagccgtaa cgtttactca
    25921 tttctcctgt tgatgcactg acaagatcga tacatcccca aggcgtatag cccatcaaat
    25981 ctacaccgtc tgctactgca ttagccatcg cttcgatatg acgacgtaaa taatcgatcc
    26041 gataatcatc aataatcgtg ccatcctctt ccatatgatc aatggctcca agaccgtttt
    26101 caacaatgaa taatggtttc tggtaacgat tgtataattc attcaatgcg attctcagcc
    26161 cttctggatc gatctgccag ccccattcac ttgcctcaag aaacggattt tttactcctc
    26221 ccagcaagtt cccttctgaa gttgctgctt gtggatcagt ctcattgatt gccgtagaca
    26281 tataataact aaatccgatg tagtccactg gatattcttt tagcaactgg aagtcttttt
    26341 ccgtttgctc taatttttcc ggatcgactc catatttttt atgggtacgc gctgtgtatg
    26401 cagggtattc tccacgtact tggacatccg tgcagaaaaa attgaattct tggttttgga
    26461 tcattgttgc tgcctgattg actggattgg catcgatgct gtaagttgtt gcatagataa
    26521 tcatgcagcc gatttggata tccgaacgca attcgtggcc gatttttacg gctttgctac
    26581 ttgcaacgaa ttgattatgc catgcttgga aaatattttg gtactctcct gctccattgc
    26641 tttttactaa tccttgactt aaagctggaa agtgaaaggc cgaattgatt tcattgaaag
    26701 tcatccaata tttgacttta ctgctgtatc tctctaatac tgtctgagca aatcgttcat
    26761 agaagtcgat cagttttcga tttttccagc caccgtattc ttttgccaaa tgaagaggca
    26821 tttcgtaatg agaaatcgtg atcacaggtt caatatcata ttttaagcac tcatcaatca
    26881 gctgatcata aaattctaat cccgcttcgt taggagtacc ttcgtctcca tttggaaaaa
    26941 ttcgtgtcca ggcaatcgaa aaacgataac atttgaatcc catttccgca aacaaggcaa
    27001 gatcttcttt gaaacgatcg taatgatcga ttcctcgatg attcgggtaa atatattttt
    27061 cttgatcaat cgtccagtca aattcttcac tgccgataat cgcaaagcgc tgcttacctc
    27121 caggcatagc atctgcaacg gatagtcctt ttccgtctcg atcataagcc ccttctaatt
    27181 gatttggcag cagttgctcc accccataaa aatttttctg aaaaacgtga ttgcatcctg
    27241 atttcctcct tattttacaa ttgccagaac atcttcgttt gcaataatct ctttttggtc
    27301 taattcaagt atatcggcaa atgcatcagt attcgtaatg acaactggag tgattacgct
    27361 atagccagct gctctaattg cttcgatgtc aaaacgaatc aataaatctc ctgctttgac
    27421 gagatcccct tgtttgacta agatctcaaa gccattccca tttaattcta ctgtatccat
    27481 cccgatatga atcaaaatct ccgctccgtc agtcgttgtg atccctacag catgtccagt
    27541 tggaaaaata gtcgtgatct ctccgtctgc aggtgcaact agttctccta cttctggatc
    27601 aatagcgata ccttttccca tcacgccaga agaaaaaact tggtctggta cttcactcaa
    27661 tggcagtact ttacccgtta aaggactttt taaggtatgc cgtgctgaag taatcggttc
    27721 tcctgcattc gcatgtttgt tttctaattg attgtcttgt gtttgttcgt caaatcctaa
    27781 aatcaatgta cctacgaatg caagaacaaa agcaattcct gtagctatta ctgctactgt
    27841 cacatttgac tctacaccat ccaccgtatt aatgaaagtc ggaatcgtca ataagctgac
    27901 caaactgctg gagaaagctt ttactccgga aaatccgata atagctccac caatacctcc
    27961 agaaatacaa gcggcaatga atggtttctt caaaggtaat gtcacaccat acacagttgg
    28021 ttcagtgatt ccaaataacg aagtcactgt agaagaaatc cctaatgctc gaagtttcgt
    28081 atcttttgtt cgcaatgcta cagctaaagc agccccacct tgtgccaaga tggctggaag
    28141 aagcattggc atcaatacat caaacccata ttgttcgata ttcaaaaaca tgatcggtac
    28201 gaatccccaa tgcatgccga acatcacgaa tacttgccat aaacttccca tgattagacc
    28261 tgctacgatt ggactaaatc cataaatcga gttgaagaga ccgcctaaag cattaccggc
    28321 aaccgtcccg attggtccta aagcaagaaa tgttaatgga accatcacta ctaaaacgac
    28381 aagcggaact aaaatcattt gcagaaattg cggaatcact ttttttacga atggctctaa
    28441 tttactttga acccatacgg ctagaatgat tggtatgaca gaagaagtat aaccagatgg
    28501 tgaaagaatc actgggatcc caaagaaact gatagcttct cccgcaccag caactgctgt
    28561 gatagctgga tacacaagtg ccatagcaat tgctactgct aaaaactggt tggctttgaa
    28621 ctttttagct gctgtaaaag caagcatgac aggtaagaag gtaaagacac catctgctgc
    28681 agcaaacaat atttgataag taccgctatc agctgtcagc cagcccatca ccgttgccaa
    28741 tgataagaat cctttcaata ccccggcggc tgccatcgcc cctagaaaag gtgtaaatat
    28801 actagagata atatcgatca gacgattcaa aatatttttg gactcttctt ttccatcctc
    28861 cgaaactgca agaccttcct catccacgat cgattgataa acatctgcta cattactgcc
    28921 aatcactact tgatactgac cgccactttg tacgacttgg atcacgtccg ggtcttcttt
    28981 taacttttgc gtatctgcta aagattcatc ttttaatctg aatcgcaacc gcgtagcaca
    29041 atgtactaaa cttttgacat tcttttgtcc tcctaccgct tcccatacac gacgaccaac
    29101 tgcttgatta tccattgttt tttccttctt tcttttttat ttaaaaaata aaaacccaga
    29161 ttgaaaaaaa cagtcacaaa cattcgtgaa cctatttttt caatccaggt tttgcctgcc
    29221 atacagtaac aatccttatg attggatgct ttttttctca cttactactc tttgaatatg
    29281 aatcgttaaa taaatctgct ctgcgatccc catttcctgt ttttgcgttt gtcgaagata
    29341 ctcattgatc cgttcgacac aatgaaacgc ttttggatac tttccttgaa ctaatgcgta
    29401 gagaaattca tcttgctcgt cagatccttc ccctttgaga taacgctggg caaaaaactg
    29461 cagatgagta atgatacgct gataattcaa agattcttca tcgaacacga tgccgaagaa
    29521 tttactgatg atactcagta tatctcttac cattgtggta gcggtcatcg tcacttgcat
    29581 gtcttcgttt ccttgttggc tattcacaaa atgaaaggca ataaaaccgg cttcatcctc
    29641 agaaagttcc acacccgtct tttgtttcac gaacaaaaga gcctctttcg ctgcttgata
    29701 ttctttcgga taaaattttt ttgtttcaaa taataatgga ttaggcagtt ggatattttc
    29761 ttttgctcgt gtgatcgcat aatgaagatg atcggtcaat gtcagataaa tattaggcga
    29821 aaattcaact tttagtttat cttcagccag ttcgactact tgtttgaccc attcgatctc
    29881 ctctgccgga atatcagcca tcagctgctg gaattggttt ttagcaaaag aatctcttaa
    29941 aacaaattct ttttggaccg cttctggcga aatctcgtcc ccaatctttt tttgaaaagc
    30001 tagtcctttt cccatacaaa cgacttcttc cccgaatcca tttttcgaaa tgacgacatt
    30061 gttattcaaa attttatcaa ttttcatttt gcttcgccct ctcaagaaaa ataaaaaaac
    30121 ctagaattcg cctactgttt cttagctgaa ttctggtttt gcctgccgta cagtaacaat
    30181 ccatttctta ttcaaaatca tcataccatc gaatttttat ttatgcaagc cttttcatat
    30241 aaaaaataaa aaaactggaa cacaagtcta tctagaaaac aaccaaatca gagatgcacc
    30301 tctttgcgtt tctctttttt tcagcaaatt ggtttttcct tatcgattca tatcctttgg
    30361 ctgtttttaa tctgtaacga cttatgtccc agtatcctaa aatgtttttg tctctagatt
    30421 gaaagcagat ctcatatcat ctgtcgcatc ttttatcgat gtaacacttt ccagttctcc
    30481 ttgtacaact agtcgcgtga cggcgtccat gtcgccacaa gtaattagtg tgacaatttc
    30541 ttttccctcg acttcattca gtaattgggt atctgtagga gcaacttttt ctttaaaaaa
    30601 tgttttgtat gtatacacat tttctaaatc tgtcaaatag atcttggcac ccatctctaa
    30661 attttctagc ggtgtaaaca gcaattcagg gttcgttgca cgatggcttg ctaaggcata
    30721 atttcctttt cccatttctt gatctggaga taatgttcct gctccgtaaa gtagtgacgt
    30781 attatccaag cctttgaaaa ccggtaaatt gattgaaact gaagggactg cgatactagc
    30841 aatcactggt agatctgttc ctctcatttg cgacctcatg accccttctg tcgtcattgg
    30901 ttctaccgca tcaaagtcaa aactgacatc tttatcgtta ttctttttca gatcttcttt
    30961 tgttacattc gctatagcat atttatctcc tgtctctctg acaaaataat ccttgatctg
    31021 ttcattgaaa atcaaagcta gtcctactaa taacaagagg aacaagagga tattgactag
    31081 ccatcttttt gcttttcctt tttttttcat tttctctccc tccagactca ttttactgtt
    31141 tttcttttgc tctttccaac agccgaatga cacatttcta caactctgtg tatttttttc
    31201 gtacacctgc gcttttttct acaggatatt tcaaattatt tttgatcaat ttttcttcaa
    31261 tgatctcatc tagatccagg tgaaggttgt ctgccatcat gtatgaatag atcaatacat
    31321 ctgccagttc ttctttgatt cgttctaatt gtgtttcttt tacttcttcc ggcgttttcc
    31381 attgaaaaag ttctaacaat tcagatgctt ccaaagaaat agagatagcc aaatcttttt
    31441 cgttatgaaa ttgtcgccaa ttacgttcat ctctaaactg gttgactttt tccatactat
    31501 tcatttgctt gccttcttcc aatcacttct tacttgatta atcataccat ttttttacat
    31561 aaaacaaagt ataaatttct aattatcttt ttatattttc ttaaatgctc gtaaagcctt
    31621 attctatgtg ctttcgagta tttttactgt aggaagatac ttcacgtttc tttgcatatt
    31681 tcctcatgtc ttagctgtca gaagtggtaa ataagtagta aattcatttg tactactaag
    31741 caacaagacg ctcctgttgc ttctctttat tcaagcgttt catttctgcc attgcagaat
    31801 cgaatgttgc atgtgcgtaa tagttcagcg tcatggctat attagcatgt cccataatgt
    31861 actgtaatgc ctttggattc attcctgcat ttgcatagtt ggtacagaat gtatgtcgca
    31921 aactatgtgg agtgatgtgt ggcaatttat cctcgttata cttattgtat ttcttaacaa
    31981 gacctttcat catgccgttg taatcacttg ccacttttgg atagttcttt ctattaagaa
    32041 agaggaaatc actatatcca tcaatctcaa cacgcttatc attctttcga ttcgctaaca
    32101 ctcgcttaaa tgcttgatag gcttcttcaa ccataggaac ttgacgttcg ccacttttgg
    32161 tctttggtgt ttcaatgtag tacccaattt cagtatctct caatagctga tggtctatat
    32221 tgacaagacg attctcaaaa tctaaatctg gaagtgtcaa accaccaaac tctgaaatac
    32281 gaagacctgt ttttaagagt atcagaattt catcataatt tttgctgtag gttttatcag
    32341 cttttgcaaa ggctaacagt ttttcttcct gttcttctgt tagtacggtc ttagggacag
    32401 tatcatcatc aagaactgct ttcagttgaa agtcaaatgg attcttccga acacaatcat
    32461 cttgtatagc aatatagaat gaagccttta aagaacgttt gtagttattg atggtttgat
    32521 aagcataacc attttcactc attctaatag cccattcttt agcgtctgat ggcttaatac
    32581 tgtcaatgct tcttacacct aacttgtctt tcttcaaaat atccataaga tatttgcgtc
    32641 cagtttcagt gttttttcta acctttggtc tttgagcgtt ctgttttgcg taaagctggc
    32701 agagtgtcat tttctttcct acaacatcaa taccatcatg aatgtctttc tgtaactctg
    32761 cgattttctc tctaagtgag atacaatcac gctttcctgc tggtactcgg tctgtagcca
    32821 caagtttcca cgagtaaaca aattgcggtt ctccaaatga atctatatat ttgtataagt
    32881 atcttccgtc ttttcgttgg ctctctccag tctttaagat tcgacctcta ttgtcacgtc
    32941 ttttttctga catggcattt gctcctttcc tttatggaaa gagccttgat acgacttaat
    33001 actattttat catatacaag accctttggc gacgctagat tgcgtccaat gtatctataa
    33061 ttttttcaaa ttgttttcgt ttaatctgaa tacgattgcc attcataatc agccaatttg
    33121 catttttatt ttcctctgcc aagcgtcgta gcttgttttc gccaatacga aaatattttg
    33181 acgcttcttc aatggttagg gtataacgtt cccaaatagg aatgtcagtc tgcttcataa
    33241 aatcctcctt tccaaatcac ttatttggat ttcataaaag ttgttttacc agcaatcgaa
    33301 cagctttagc aaagctcacg ggagttccac ccctgcatgg ttctcatgta gccatactca
    33361 ttgcctgcga cggttttatc acgctcggac tattgactgt atgggagtat cattatcacg
    33421 ataagaatgt cgttgcaggc aatcctgcta aagattgctt ctcggatcac taacatgaat
    33481 cgctcgctat ctttataaga taggtcatgg cggttagttc cgttggctct tttcttatcg
    33541 aaacgtattc gattactttt attcagtttt caaagaacaa tggctcgtta gcctatcaaa
    33601 acacattgaa agctcaatat gctttggtgg aataacaaac ctccctgttc gggaagcgtg
    33661 gaatggttta gcacgcttcc acgaaaggag agaggatatt acttaatttc aaatgacaaa
    33721 atctttgtaa tcagtctggt ttccattctt ccacgtaaga cttcatcaac gaccatactt
    33781 tgattgccat attcatcttt cataagtcgt agggaacgct tcgttatgta ccctctgtaa
    33841 tgatgtagaa tctggttaat cgcttcggta tcgccatctg ttgcctttac aatgagagga
    33901 aagggaatca taggatattg tgttttcatt cttcaaattc ctccataaac tttttaatta
    33961 aggctagtcc actggttcta tgccgataga cagtagaacg gttcaatttc aacaggtctg
    34021 caatttctga atcgctcatg tccataaagt aaaacagcag tagaatttca cgtttcttgt
    34081 ctggcaactc acgtaatgct tcactcaaca aatcattttc aacgcctact gataacccat
    34141 tgagtgtaaa aatctgaaag tcagttgaat agttatctgt tgtcgcaaac tggctaacaa
    34201 gataatcgcc aacatccgaa aaggacacct cacgctttgc aatccttgaa agataaagca
    34261 tataattctt tcgctcgtct tccatagcac gtttacagat atagtcaaac tgattttcta
    34321 ttgtggtctg aaaagaagat ggtttcatgt ttctcacccc ctttctgtct aggaaaggaa
    34381 gtgagccttg ctcgtttatc tcctttcact cttagtccca atgtgaaagg gggatttgtt
    34441 gcattactga taaataaact ttgtaaaaaa gttctgaata gccaaaaaag catataaaca
    34501 gatttatttc tctgtttaca tgcttctgtt attctatcta tatgatttat aaaaccacat
    34561 tggtggacgt acttatctat tgcagataga cgactttttt tgacaagaac ccaatgtaag
    34621 gaaatttatt gtatatgatg tacttcatgg cgacgttgac ctccaacaaa ccgccatttg
    34681 gaagtaatat acaatatttt aacagcgtaa atagcactac catataacgg ttttttttat
    34741 tggcgtttag tagtgctttt tattaaatat aaacctataa accatataac acgtttttct
    34801 atacctgttt ttaattcagt aggaacaata aaatgtatag aggtggtcta ctatgcgtaa
    34861 aaaagaagat aaatatgatt ttagagcctt tggtttagcc attaaagaag ctcgattgaa
    34921 acgaggttta actcgtgaac aagtgggagc attgattgaa attgacccac ggtacttaac
    34981 taatattgaa aataaagggc aacaccccag catacaagtt ctttatgacc ttgtatcgtt
    35041 acttcatgtt tccgttgatg aatttttctt acctgctaat aacttggtaa aaagcacccg
    35101 acgattacag atagagaaat acatggatag ctttacagac aaagaactat ccataatgga
    35161 atctttagcc agcggtatca acgaagcaag aaacatcgaa gactaattaa aagaatccat
    35221 acataacgga aagagccgat aaaatgagat tgtattaatc tcattttatc ggctctgcgt
    35281 ctttgcgtct ggctctgtaa tcacagttac tttgaactgc tttatttcaa ttaaattttc
    35341 ttgtctgcat ttcggacaat agagggggaa tttttttaat tcagtatctt cccttatctt
    35401 taatcgtgtt ttatttccac atacaggaca caatatccac ttgtagttta taataactat
    35461 ctcctccttt acactttaat tcaaatcttt attaaaaaat atttcatctt atttaacaag
    35521 aaaccatatt tatataacaa cataaaatac actaagttat tttattgaac atatatcgta
    35581 ctttatctat ccgactattt ggacgacggg gctggcaaac aggttcaccg gtagtaacat
    35641 ggtacccttt taactctgtt aaacaaacac tacgtccatt tgtaaagaaa gttaaatcac
    35701 tacgatattc ttgaatacac cgagcaggga tttctccact aagaatgacc tcattatttt
    35761 tcaattgagt gtctacgatg ttcgcacaat atttaggagc atcgttgtat gctcgtgaaa
    35821 gatattcctg tggcgcataa attttaaaac taagatatgg ctctaacaat tctgttccag
    35881 ctttttttaa gacttgttcc aatacaatag gagcaagcat ccgaaaatct gctggggtac
    35941 taacagggct atagtataag ccatacttaa aacagatttt acagtccgtc acattccaac
    36001 catacaatcc ttgttcacaa ccatagcgta tcccttccat aactgcattt tgaaatgatt
    36061 gatttaagta tccaagagaa accgagctct catactgcat tccacttccc aacggaagcg
    36121 gtgatacaga taaaccaatg gaagcccaga aaggatttgg cggcacttcg atgtgaatgg
    36181 tatattctgc attttttaac ggtctctcca tataaatgac tgtaggctct tttagttcta
    36241 tctccacatg atacttttct tgcaacagtg cactaatcac ttccatttgt actttcccta
    36301 agaaagaaag tataatttca tgtgtcgtag aatccacgta atatcgtaga agcggatcac
    36361 tatctgagat ttccaaaagg gcatcaagca acatttctct ctgttcaggt ttactcggtt
    36421 caacagttgt ttgtagtaga gggtgcggat tttcaatctt ttttctctgt ggcaatagtt
    36481 ttgtatctcc aagaacacta tttaacttca aaaactcatt ttgcaaaata acaatttctc
    36541 cagaataagc tctatcaatc ttacataatt caccatttat tgaagtatac atttctgtaa
    36601 cttttatttt ttctttttct gatactctaa ccgaatctcg taaatgtagt actccactat
    36661 aaaggcgtat atatgcaaga cgttgtcttt tttttgtata ttcaattttg aaaacatttc
    36721 cgcaaagttc agacggacct cgatgtgttg atgaataaaa tttattagta ataacttcta
    36781 taaggttatc aatccctata ttactttttg cacttccatg ataaagaggg aacagagaac
    36841 aattctgaaa tcttatgctt tcctcttgtt cgagttccaa tgcttctaat gatttaccgg
    36901 acatatattt ctctaaaagg tcatcgtttc cctctattac cgtatcccat tgttcagatt
    36961 cggtaaagtt cgtcacacac acattaggat acagttctac cttctgtttg attacaattt
    37021 cggcagaaag tttctcttta atatcctgat aaaccgttga taaatcaatt ccattttggt
    37081 caatcttatt gataaaaaag attgtgggaa tccccatttt cctaagtgca tgaaataata
    37141 tacgagtttg tgcttgtacg ccatcttttg cagaaatcag tagaattgcc ccatctaaaa
    37201 ctgataatga acgatatact tctgctaaga aatccatatg tcctggcgtg tctatgatgt
    37261 tcaccttcgt attttcccac tgaaaagagg ttattcctgt ctgaattgta attcctctct
    37321 gacgttctaa aagcgtatta tccgtcctcg ttgtaccttt gtccacgctt cctaattctg
    37381 taatcgctcc actgttatat aataagcttt ctgttaaggt agtttttcct gcatcaacat
    37441 gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa aagcccaaaa
    37501 gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc ataaccatag
    37561 gcatacaaag catacagata ttctccggat actttagaat cacatgataa aggtattctt
    37621 aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt tgttcccgct
    37681 atcaaattga cagtttattt aataatacct tgccgcatat ttattaactc cttttaaata
    37741 gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat tttttatcaa
    37801 agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta ttctataatc
    37861 ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg cgttgctttg
    37921 gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc cttacgattg
    37981 tgaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt taatatcccg
    38041 tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt gaagaacgtc
    38101 gttaaaaact cattgatttc attggttgta atggaatcaa ccgtcccctc actttcaatg
    38161 gcttttggtt tataacttga tttcttaggt atgttggtaa tggtcggatt cttaaccagt
    38221 accatatttc cagaaccatc tacatagaca ctcactatat aagcagagtg gacggtcttt
    38281 gtattttctc cctctgtaat gagctggtct acactgtagg ttacattaaa ctcattgtcg
    38341 ccagttggct ctaccgtcca tatctgaaat cctcttacag aagacgatac aggaatatct
    38401 ttgcgtactg tatcaacatt gagagcttga agttcatctg tcagatagcc ttttagactt
    38461 tccattcgat tatcaatgga cttatcggat tgctcccatg aatagtagac tttcgcaaag
    38521 ttctctacaa aattttctac atgatgagta tcaacgtatt ccttttctat gatagttgtt
    38581 tcgtgaatag tatgagtatc tatagctgta aagtgcttga atatcgcaaa gctgaaacta
    38641 agccctaaaa gtacccacaa ggcaatcaca acctttttat gaggattgac cttatagaca
    38701 cgaggtttct tttcctttgg tatctgtttt tctttattct gattttttct aaatttcatc
    38761 attaaatctt cctttctcat tgtttgattc gtcctgctcc cactaaatgc tgttgccagt
    38821 aggggcttgt taagtcggca taaccgattg ggtcgcctgc atgaaacata cggttattgc
    38881 caaggtatat cccaacatga gtaatataag agccagcgtt ataggtagaa tgaaagaaaa
    38941 ccaaatcgcc agcttgtgct tccgatagtg ggatatgctg ggtcacatca tattgctgtt
    39001 gtgcggttcg tggtaagtta attccagctt ttccatacgt ccattgtgtc agtccgctac
    39061 aatcaaaaga agtagtcggg gaagctccac cgtaaacgta tcgccagccc tcatatttca
    39121 gtgcttcgtc catgatggct tgtaccgtat catcatcaaa ctctgttgtg acaagatact
    39181 gcgttaccag ttgcacataa aacatattgc catagttgta tcgccagccc ccattgatag
    39241 gtatggctat gggattgggg taagacactt tttcgccacc tgaatactct tttgagaaac
    39301 tttgagccag ttcaaaggta tatttatttc cacgattagc cacataccct aagaaaccac
    39361 caccataatt gtaggactgg ataaccgatt ctaaatctac actgagcctt tcgctactgg
    39421 ctaataattc actgaaatac ttcacacctt gcttaatgga ttcttctgta ctcaatgaat
    39481 taggtggaag accgagggat tccgaggact gcataacatc ttccgcagta ccgcccgatt
    39541 ccacctgtat aatcgcaaga agtatgttga catattcttc aacgccatat tctttggcat
    39601 atttttctac cataggctta tgagccagca cttctgcgga aacattcaca cctccataat
    39661 gaatattgga aattccgctg tcctgttcat ctgaaaataa aatggcaaca aacagaagca
    39721 gtgagaagac catcaagaat aatccagaac caccaatcac taaagttttc aacttcatgg
    39781 tttcttaccg actttcttaa tggtggcggt tttgattggt ggtctacttc ttgtattttg
    39841 tagtggtact ctttgaacag tagacggacg ttcttttgtg attggacgtt gtgaagttct
    39901 atctgctgta gtggttgaag ttgctggctt ttgaacggtt ttttcttgaa cggtattgcc
    39961 ttggcgttcc acttttggac ttgaaaaatc ggacttaact gctggacgct cttgtttggc
    40021 ttgttgagat tccttatatg aagtctgaat attagactgt tttgaggtct gttcatcatg
    40081 atattgttct tgtcttgtag tcggtctttc atgaacagaa gaagcaggct gttttttctg
    40141 tttgacctgt tccatttcag agcgacgctt cgcaatggtt tttcgccttt gttcctgctg
    40201 ttccttgcgt ccactggctc tgtccgcttt ggtttgagaa atactactgg ttaaatcacg
    40261 gacattctct tttactttgg attttccttg atatactgca tatcttgcat tggtcggcaa
    40321 atctttaacc tgttctttca aaccactagc agtgtctacc attctgtctt tggtatcagc
    40381 tactgtaccg atggtttgac cgatacgttt tccaagtgtt gatttttcct ttccgtctgg
    40441 tcgggagtga tctgcttgtg tccttgcaga actccccgaa cccgactgtc cttttttacc
    40501 tgtaacaatg gcagacccag cccctagagt agtcatggaa cgtccaagtt tccgctgtag
    40561 acggtgcatg tgagcgtgca taagcatacg aggttttctc atcacacgac ttcccacact
    40621 ttgagaatcg ttactctgta gagaaaacat actcattaaa tcgcccagct tgaagtagat
    40681 tcctgcaaag gtcacaatct gtagaaaagc aatcaaaaag aacggataac cagccgataa
    40741 ggtatagagc atggttgaaa tactaaatgc tgtcgtaata atcaatgtga ttccagctcg
    40801 tgtcaaaatg gtattaaaga gctttgttat ggctcgtttt gacataccat caaatgatgg
    40861 aatcatgctt aaaataaagc tcacaggcag aaacatagca tagatgataa aaagtacctg
    40921 cgagaaaatc atgattcctg ttaataggaa tacaaatatg gaaatcccaa tattgaagac
    40981 aaataggaag aagactgtac ctaaacggtt aatggtcttt gtaatggtta gattggtatt
    41041 gcttctgtct tcaatttctt ccgcaacaat tttttctctg tcttcgccat tgttggaatc
    41101 tgggctggtg gagagcaggc tttccacacg gtcaataccg atactttcaa tgtctgaact
    41161 gttgtattga agcagtagcc acggttgctg aacctgtatg gaaaacaggc tatctctgat
    41221 taagtccacg ctgtccttgc cttgactatc ggaatggggc atgacaatct tcgtgccaag
    41281 tgataaactg gcattactga tgtctgatga aaagtcattg atttttttaa tgtagtcggg
    41341 agcgtaggca ataaaggaag ccgataggat aaacaccagc acaaaattca taatggcatg
    41401 aattgccttt gtggtttctc tctttatcag tcccgtatag gcaacataaa ccccaagaac
    41461 caaaatcaag agtaagagga atccaacata gaaaccctct gttgaaaatc cgtttgcact
    41521 cacaccagct aaggtctgca tattcttacc aatggaatct gctgtagcgg aaatgaagtc
    41581 taaggaatag gcttcctgta ctaagtaacc tgtcgcattg gaaacataca aactgattgt
    41641 ccaaataaaa ttggtaatgg catatagtcc atacatgacc tgttttccaa tcccgtccga
    41701 ccagttccac ggaagccagc cccagctatt atccacataa aaatccagtt gatagttttc
    41761 aagtgggtat cggctgtatt catttgccac attgaccgta tcatctacca agcccgcagc
    41821 ttgaaccacc gttcccagca tggctaaaag aaaaatggca atcacaagtg tgaaagccac
    41881 tgtcattgcc actttaccta gacgtttcag cgtccagttt gattttattc tgtttactat
    41941 tgatggtttc acatttacac ctcttttcgc acaggtggtc tggtatcaaa ggcatggagc
    42001 agttcttcaa atacagggtg gaactgtatc acaccgacac gaccatataa atcactgata
    42061 aggcattgcc cgttttccaa atcacgcaat cgcttctgat tgttttcgtc ctctgggtct
    42121 acaccaaaaa aggctaaggt ctttttaatc tcgttaaggt cagtggaacg aaatgcaaat
    42181 tttaagccga ggttattttt cagtttttca tctaagaggt cgtctgtatt ttgggtcacg
    42241 aaatataccc cagcgttcat agcacgacca gcccgaacca gcttcataga tagtgttttt
    42301 ccttgtgcta cctgtaaaaa gctccatgct tcgtctaaat ctacaatctt gaaaatgctt
    42361 cggtctgtat ggataaagtc taaagcaaag gtactaatga caatcagcat agcaacggat
    42421 aaaagctcca tagtggtata ttcctcaaag gaagtttcct tgtcgggaag taccaagtcc
    42481 gcaacctgta taatgttcag ttgtttttct aagctgatag actgctccac ataaccatta
    42541 ctgaataata aatgtgcaaa gtcatagtct gtaaaacttt cgatatggtc ggctatactg
    42601 gtacttagtg gcgtattctc aacccgtaat tcctcaatca ctttcatcaa ccctcgtact
    42661 tcactattgg ttactgcacg aatggctttt ctaaggattg ggaagcgttc cccatcacga
    42721 gaggaaatcc ccgtaaggaa tgtcagaata tcaatagcca gtgattcaga atctttggga
    42781 tttttcataa tcacataagg gtcaagtaag cctttgtttt tctcatcaga agtcagagtg
    42841 acgatattga tttcatggga aatctctggc aaggtttctt tccatctgcc
//
LOCUS       DAJSNV010000015        42510 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSNV010000015 DAJSNV010000000
VERSION     DAJSNV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.016"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..1130)
                     /gene="recA"
                     /locus_tag="ON516_000785"
     CDS             complement(81..1130)
                     /gene="recA"
                     /locus_tag="ON516_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3344285.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1239..2480)
                     /locus_tag="ON516_000786"
     CDS             complement(1239..2480)
                     /locus_tag="ON516_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3344286.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2720..3298)
                     /gene="pgsA"
                     /locus_tag="ON516_000787"
     CDS             complement(2720..3298)
                     /gene="pgsA"
                     /locus_tag="ON516_000787"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3344287.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3507..4430)
                     /locus_tag="ON516_000788"
     CDS             complement(3507..4430)
                     /locus_tag="ON516_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344288.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5169..6455)
                     /locus_tag="ON516_000789"
     CDS             complement(5169..6455)
                     /locus_tag="ON516_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3344289.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6448..7716)
                     /locus_tag="ON516_000790"
     CDS             complement(6448..7716)
                     /locus_tag="ON516_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3344290.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7968..8429
                     /gene="mscL"
                     /locus_tag="ON516_000791"
     CDS             7968..8429
                     /gene="mscL"
                     /locus_tag="ON516_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3344291.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8488..8934)
                     /locus_tag="ON516_000792"
     CDS             complement(8488..8934)
                     /locus_tag="ON516_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344292.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9027..9272)
                     /locus_tag="ON516_000793"
     CDS             complement(9027..9272)
                     /locus_tag="ON516_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3344293.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9387..9965)
                     /locus_tag="ON516_000794"
     CDS             complement(9387..9965)
                     /locus_tag="ON516_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3344294.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10042..10572)
                     /locus_tag="ON516_000795"
     CDS             complement(10042..10572)
                     /locus_tag="ON516_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3344295.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10576..11436)
                     /gene="rlmB"
                     /locus_tag="ON516_000796"
     CDS             complement(10576..11436)
                     /gene="rlmB"
                     /locus_tag="ON516_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3344296.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11429..11824)
                     /locus_tag="ON516_000797"
     CDS             complement(11429..11824)
                     /locus_tag="ON516_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3344297.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11821..13233)
                     /locus_tag="ON516_000798"
     CDS             complement(11821..13233)
                     /locus_tag="ON516_000798"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3344298.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13230..13766)
                     /gene="cysE"
                     /locus_tag="ON516_000799"
     CDS             complement(13230..13766)
                     /gene="cysE"
                     /locus_tag="ON516_000799"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3344299.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13852..14091)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14088..15548)
                     /locus_tag="ON516_000800"
     CDS             complement(14088..15548)
                     /locus_tag="ON516_000800"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3344300.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15718..16875)
                     /locus_tag="ON516_000801"
     CDS             complement(15718..16875)
                     /locus_tag="ON516_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3344301.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16909..18279)
                     /gene="radA"
                     /locus_tag="ON516_000802"
     CDS             complement(16909..18279)
                     /gene="radA"
                     /locus_tag="ON516_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3344302.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18342..18869)
                     /locus_tag="ON516_000803"
     CDS             complement(18342..18869)
                     /locus_tag="ON516_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3344303.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19099..19905
                     /gene="proB"
                     /locus_tag="ON516_000804"
     CDS             19099..19905
                     /gene="proB"
                     /locus_tag="ON516_000804"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3344304.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19902..21149
                     /locus_tag="ON516_000805"
     CDS             19902..21149
                     /locus_tag="ON516_000805"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3344305.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21225..21569
                     /locus_tag="ON516_000806"
     CDS             21225..21569
                     /locus_tag="ON516_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344306.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21686..22012)
                     /locus_tag="ON516_000807"
     CDS             complement(21686..22012)
                     /locus_tag="ON516_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344307.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22627..23829)
                     /locus_tag="ON516_000808"
     CDS             complement(22627..23829)
                     /locus_tag="ON516_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3344308.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23946..24461
                     /locus_tag="ON516_000809"
     CDS             23946..24461
                     /locus_tag="ON516_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3344309.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24660..25106)
                     /locus_tag="ON516_000810"
     CDS             complement(24660..25106)
                     /locus_tag="ON516_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3344310.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25153..26631)
                     /gene="msr(C)"
                     /locus_tag="ON516_000811"
     CDS             complement(25153..26631)
                     /gene="msr(C)"
                     /locus_tag="ON516_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3344311.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26985..27614)
                     /locus_tag="ON516_000812"
     CDS             complement(26985..27614)
                     /locus_tag="ON516_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3344312.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27598..28062)
                     /locus_tag="ON516_000813"
     CDS             complement(27598..28062)
                     /locus_tag="ON516_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3344313.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28549..29817)
                     /locus_tag="ON516_000814"
     CDS             complement(28549..29817)
                     /locus_tag="ON516_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3344314.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30048..30785)
                     /locus_tag="ON516_000815"
     CDS             complement(30048..30785)
                     /locus_tag="ON516_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3344315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30852..31421)
                     /locus_tag="ON516_000816"
     CDS             complement(30852..31421)
                     /locus_tag="ON516_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3344316.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31549..32421
                     /locus_tag="ON516_000817"
     CDS             31549..32421
                     /locus_tag="ON516_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3344317.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32763..33635
                     /locus_tag="ON516_000818"
     CDS             32763..33635
                     /locus_tag="ON516_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3344318.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33632..35590
                     /locus_tag="ON516_000819"
     CDS             33632..35590
                     /locus_tag="ON516_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3344319.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35922..36773)
                     /locus_tag="ON516_000820"
     CDS             complement(35922..36773)
                     /locus_tag="ON516_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3344320.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36896..37363
                     /locus_tag="ON516_000821"
     CDS             36896..37363
                     /locus_tag="ON516_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3344321.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37626..38108)
                     /locus_tag="ON516_000822"
     CDS             complement(37626..38108)
                     /locus_tag="ON516_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3344322.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38208..39071)
                     /locus_tag="ON516_000823"
     CDS             complement(38208..39071)
                     /locus_tag="ON516_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3344323.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39669..40544
                     /locus_tag="ON516_000824"
     CDS             39669..40544
                     /locus_tag="ON516_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344324.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(40689..40841)
                     /locus_tag="ON516_000825"
                     /pseudo
     CDS             complement(40689..40841)
                     /locus_tag="ON516_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(41109..42440)
                     /locus_tag="ON516_000826"
     CDS             complement(41109..42440)
                     /locus_tag="ON516_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3344325.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        12257 a         8950 c         7579 g        13724 t
ORIGIN      
        1 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
       61 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
      121 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
      181 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
      241 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
      301 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
      361 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
      421 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
      481 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact
      541 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
      601 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
      661 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
      721 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
      781 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
      841 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
      901 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
      961 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
     1021 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
     1081 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
     1141 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
     1201 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
     1261 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
     1321 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
     1381 gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
     1441 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
     1501 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
     1561 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
     1621 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
     1681 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
     1741 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
     1801 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
     1861 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
     1921 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
     1981 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
     2041 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
     2101 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
     2161 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
     2221 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
     2281 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
     2341 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
     2401 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
     2461 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
     2521 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
     2581 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
     2641 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
     2701 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
     2761 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
     2821 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
     2881 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
     2941 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
     3001 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
     3061 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
     3121 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
     3181 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
     3241 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
     3301 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
     3361 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
     3421 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
     3481 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
     3541 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
     3601 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
     3661 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
     3721 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
     3781 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
     3841 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
     3901 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
     3961 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
     4021 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
     4081 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
     4141 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
     4201 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
     4261 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
     4321 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
     4381 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
     4441 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
     4501 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
     4561 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
     4621 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
     4681 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
     4741 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
     4801 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
     4861 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
     4921 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     4981 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
     5041 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
     5101 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
     5161 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
     5221 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
     5281 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
     5341 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
     5401 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
     5461 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
     5521 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
     5581 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
     5641 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
     5701 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
     5761 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
     5821 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
     5881 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
     5941 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
     6001 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
     6061 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
     6121 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
     6181 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
     6241 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
     6301 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
     6361 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
     6421 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
     6481 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
     6541 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
     6601 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
     6661 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
     6721 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
     6781 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
     6841 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
     6901 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
     6961 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
     7021 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
     7081 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
     7141 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
     7201 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
     7261 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
     7321 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
     7381 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
     7441 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
     7501 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
     7561 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
     7621 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
     7681 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
     7741 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
     7801 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
     7861 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
     7921 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
     7981 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
     8041 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
     8101 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
     8161 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
     8221 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
     8281 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
     8341 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
     8401 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
     8461 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
     8521 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
     8581 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
     8641 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
     8701 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
     8761 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
     8821 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
     8881 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
     8941 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
     9001 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
     9061 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
     9121 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
     9181 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
     9241 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caactatacc
     9301 acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat
     9361 aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt
     9421 attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat
     9481 tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt
     9541 tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaattgct
     9601 aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg
     9661 tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga
     9721 gaatagattt gattgcttat aacgctttta taataacttc caaagctaac acctttgctt
     9781 ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca
     9841 gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt
     9901 tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca
     9961 gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat
    10021 cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga
    10081 caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat
    10141 atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata
    10201 agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt
    10261 cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact
    10321 gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg
    10381 ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt
    10441 cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt
    10501 tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag
    10561 ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc
    10621 agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc
    10681 atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc
    10741 aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac
    10801 ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac
    10861 agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc
    10921 tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga
    10981 cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt
    11041 ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag
    11101 aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca
    11161 tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg
    11221 tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc
    11281 atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg
    11341 atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg
    11401 tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac
    11461 accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga
    11521 gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat
    11581 ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt
    11641 cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg
    11701 cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga
    11761 tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc
    11821 tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc
    11881 tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt
    11941 tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat
    12001 accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga
    12061 aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat
    12121 cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc
    12181 taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa
    12241 tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt
    12301 cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac
    12361 ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact
    12421 cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa
    12481 ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa
    12541 atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga
    12601 acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc
    12661 aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc
    12721 ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc
    12781 ataattttca aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc
    12841 tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg
    12901 atgcagtgta gcaggttcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa
    12961 acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc
    13021 gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg
    13081 tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac
    13141 agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga attcttcttt
    13201 ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg
    13261 attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac
    13321 acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc
    13381 gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt
    13441 cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt
    13501 tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct
    13561 ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa
    13621 aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca
    13681 gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca
    13741 tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag
    13801 gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac
    13861 gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct
    13921 gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc
    13981 gccaagacca ctcccagagg catttcggca accattcatt gataccttgc accacccggt
    14041 atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact
    14101 tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct
    14161 ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt
    14221 acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc atctactact
    14281 tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca
    14341 agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga
    14401 tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc
    14461 cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc
    14521 agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat
    14581 tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt
    14641 ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat
    14701 gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat
    14761 tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat
    14821 gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt
    14881 ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca
    14941 tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttggat gacaaagtcg
    15001 ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat
    15061 tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct
    15121 gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt
    15181 gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct
    15241 cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga
    15301 cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa
    15361 gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc
    15421 tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca
    15481 gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact
    15541 ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca
    15601 gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta
    15661 aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta
    15721 ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg
    15781 tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac
    15841 ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc
    15901 aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt
    15961 ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg
    16021 tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc
    16081 taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc
    16141 atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc
    16201 gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta
    16261 aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata
    16321 aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc
    16381 tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt
    16441 gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc
    16501 cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc
    16561 catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat
    16621 cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat
    16681 aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa
    16741 caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca
    16801 agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat
    16861 gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac
    16921 acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc
    16981 ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc
    17041 tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc
    17101 gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc
    17161 gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc
    17221 atcttgattc tgaagaagca acccagctct tttttccagc actgccatga tcagagatac
    17281 ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac
    17341 taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga
    17401 gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc
    17461 caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt
    17521 tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc
    17581 caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac
    17641 gataaaaatg gctatcccat ttgttttagc ggttttgagt agttctgcag tggtttctct
    17701 tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga
    17761 atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc
    17821 tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt
    17881 tatctgctct gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa
    17941 ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa
    18001 gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt
    18061 tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc
    18121 tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat
    18181 cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca
    18241 attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct
    18301 aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca
    18361 aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag
    18421 atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg
    18481 aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca
    18541 cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc
    18601 tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc
    18661 aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc
    18721 tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca
    18781 tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta
    18841 gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa ctactcctat
    18901 tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa attctgctat
    18961 cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta caaaaaaact
    19021 attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta atgagtggat
    19081 atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat tattgtcaaa
    19141 gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc aatcgatcag
    19201 ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt agtctcatca
    19261 ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac tttgattcct
    19321 gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta taatcaaaga
    19381 ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt ggtagattat
    19441 cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat ggacacgatc
    19501 cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct gacaaaattt
    19561 ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga ccttttgatc
    19621 gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc cgaggctcag
    19681 atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg tggtgctggt
    19741 agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag gatatttgaa
    19801 gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt tgatattctt
    19861 gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac ttgatgcaat
    19921 taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca aaccgcaaaa
    19981 atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg atcattgaag
    20041 caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg atgcaagacc
    20101 gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa aaaattgctt
    20161 cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac ggattgagaa
    20221 tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat ttacgaatct cgtccaaatg
    20281 tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc cttcgcggtg
    20341 gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa gtactggtca
    20401 gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat gaaactgcag
    20461 gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc ggagcaaaat
    20521 taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt acaggaaata
    20581 atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc atcgtcaatg
    20641 caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc cattctgaga
    20701 tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg gtctctttac
    20761 gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa gacacagatt
    20821 gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct ttggatgaag
    20881 caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg acagatagtt
    20941 atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat gtcaatgcct
    21001 ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt atctctactc
    21061 aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca aaatatatca
    21121 tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt cccgcctttt
    21181 gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt gaaattttaa
    21241 tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga gtcggtattt
    21301 tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa aaatggtcgc
    21361 agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt agttacatgg
    21421 ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat tcaatcgctt
    21481 atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat cagctgaata
    21541 aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta gagtgccttg
    21601 aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt gtcacagcct
    21661 ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt tcctcttcgt
    21721 taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc tcatccatgg
    21781 acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc tgttcccgat
    21841 acacctgata cataccagct attttttgct gcatcgcttt tctagtttca ccttccgcat
    21901 tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct gaaaattcaa
    21961 tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc atctattatc
    22021 cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc cagtttcatt
    22081 attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt tccgaataaa
    22141 tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac atgagaaact
    22201 gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa cctcttggta
    22261 aacggcgttc aatcagctga ccctcggtat tagctcaaaa aacggtaaaa tatgagaagc
    22321 tatttttccg ttttgtttct ctaatactta ggataaaacg ctgattttac aacctctgtt
    22381 aaacggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa atatgaggaa
    22441 ctatttttcc gtttgtttct ctaatactca ggacaaaacg ctgattttac aacctcctga
    22501 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag
    22561 aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct
    22621 gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac
    22681 actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc
    22741 aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct
    22801 ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt
    22861 attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat
    22921 agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat
    22981 tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc
    23041 cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa
    23101 taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag
    23161 tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt
    23221 tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat
    23281 caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac
    23341 tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc
    23401 tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt
    23461 cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc
    23521 aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt tcaaaccgat
    23581 ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt
    23641 aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat
    23701 tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc
    23761 tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa
    23821 tatttccatc cctttctctc tattagcatc atacatgatt tattttacat gtcaatatgc
    23881 acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga
    23941 tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg
    24001 ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata
    24061 caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact
    24121 ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca
    24181 aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt
    24241 cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca
    24301 aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat
    24361 ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt
    24421 gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc
    24481 tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga
    24541 aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg
    24601 cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat
    24661 cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac
    24721 tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat
    24781 tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct
    24841 aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt
    24901 agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc
    24961 accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc
    25021 cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt
    25081 attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc
    25141 atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc
    25201 ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt
    25261 tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt
    25321 agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag
    25381 acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa
    25441 ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac
    25501 aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa
    25561 gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt
    25621 tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa
    25681 atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat
    25741 gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg
    25801 ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt
    25861 ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccagtctgat
    25921 ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc
    25981 ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg
    26041 agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg
    26101 gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc
    26161 atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat
    26221 actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga
    26281 aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt
    26341 ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc
    26401 gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc
    26461 gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc
    26521 tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg
    26581 gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac
    26641 actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt
    26701 gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc
    26761 aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc
    26821 atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa
    26881 acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac
    26941 gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc gaacatagtg
    27001 atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat
    27061 aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct
    27121 gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat
    27181 tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa
    27241 ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc
    27301 cctgattcgt aagcttggtt gtagatttct tcgccatatt cgtaacattg catatcgcca
    27361 aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga
    27421 cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg tttcatattg
    27481 acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt
    27541 ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca
    27601 tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt
    27661 attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga
    27721 tagcacggaa cctgtatctt tatggtccat ttcacaatgc ttgattttct tctgggcttt
    27781 ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata
    27841 gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt
    27901 ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta
    27961 tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa
    28021 tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat
    28081 aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga
    28141 atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa gaaatttttt
    28201 gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga ttattgattc
    28261 ttatcaaact atagaagata gctcttctgc agtagataat tttacaccgc caaaaatttc
    28321 cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt atgcttaaac
    28381 agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa
    28441 aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt
    28501 tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt aataaccata
    28561 aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt ccttagcact
    28621 caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat aaattggctt
    28681 ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg ttaaagagtt
    28741 gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt ataaacggta
    28801 gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc agcctacagt
    28861 tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg tatgacggtt
    28921 atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg tcagattata
    28981 ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat aacgacctgt
    29041 tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac cggaatagta
    29101 gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt gcaatgattc
    29161 tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc cattcacata
    29221 ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc caaatagatt
    29281 gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga tggcttgagc
    29341 aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag ttgaggcaat
    29401 actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag catagccagt
    29461 atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt gcccatctgt
    29521 caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg ctgatgtact
    29581 gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg aaattgtatt
    29641 ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc ctgtttctga
    29701 agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca ctgttgtgtt
    29761 ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg ctttcatctt
    29821 ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa aagtaaaagc
    29881 aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc tttttctgca
    29941 taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga tggtcgtttg
    30001 aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta tacttcctgt
    30061 gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc tatcgcaaat
    30121 gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc ttcatgaagt
    30181 tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac agctcctgga
    30241 gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc ttccatgatt
    30301 gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac aacatgtccc
    30361 gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat tggaagtacg
    30421 gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg ccattcatca
    30481 cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta caatacatcg
    30541 attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc aaaatctctt
    30601 acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat tgcttttagg
    30661 cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa tagcttcgtt
    30721 gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc tgataaatca
    30781 ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta tctcttattt
    30841 catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg gattcctgca
    30901 acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac aatggcattc
    30961 tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc atttttcttg
    31021 atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag atggttcacc
    31081 gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat gccacctaag
    31141 tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat atgtttgccg
    31201 aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc actaatgata
    31261 ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag cgctgctaaa
    31321 ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc cgttactcga
    31381 atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc tccttttatc
    31441 cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact taactattcc
    31501 ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat ggatattcgt
    31561 atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc agcaaaatct
    31621 ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga agaacttggt
    31681 gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg tcgatatctt
    31741 tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat
    31801 gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc aatctctttc
    31861 atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt tcatttatat
    31921 agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga ttttggactc
    31981 gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc ccaagatcgt
    32041 tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat ccatccgcag
    32101 gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag tcaactagct
    32161 gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa cttattgtac
    32221 aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga tgggatcatc
    32281 aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca agcttcactg
    32341 aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc
    32401 ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt tttaacaacg
    32461 aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt tgttccttcc
    32521 ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt gacaaaacat
    32581 aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac tagatcatac
    32641 taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt gtctattttt
    32701 gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc
    32761 gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac ggtggacagg
    32821 aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat ttgatttttg
    32881 atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt
    32941 tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt gatcatgcgg
    33001 aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc gaccacattg
    33061 ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt ttaagagaaa
    33121 tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag tatctgacaa
    33181 aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc catattcaca
    33241 gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg attgtatctg
    33301 acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa ccattttcac
    33361 tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc catgcgcaac
    33421 aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct
    33481 ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga
    33541 aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca atcgtacaaa
    33601 caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac gaatgggacg
    33661 ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag ggatcggaaa
    33721 ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg gcggtaacgc
    33781 agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga tgtcagtgga
    33841 caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag taaatgtttt
    33901 aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg atccatcttt
    33961 tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg cagctcttta
    34021 taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg gcaagcgtct
    34081 agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg
    34141 gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt taggctgggg
    34201 tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat tgcatcaagc
    34261 aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta aatttttggc
    34321 ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc caatcatgat
    34381 gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag aaaacaaagc
    34441 aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg gtgtaacaga
    34501 accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc atgcaatttt
    34561 cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat ttgcctttag
    34621 tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc agccattgat
    34681 gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta actttgctat
    34741 caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag atgacgatac
    34801 tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct acgctgcatt
    34861 aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc gtttgcaagt
    34921 caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac ctggtatgaa
    34981 gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga
    35041 tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa acaccgtggt
    35101 taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta tcgcaaatgg
    35161 gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga
    35221 cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg gtacgatcac
    35281 aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta tcgaagtctt
    35341 gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt tatatgtaaa
    35401 agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg ccgctattca
    35461 atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca aagtccaaag
    35521 cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa
    35581 caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca ggagctgaga
    35641 caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg ccaactccgc
    35701 ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga ctcttcttat
    35761 tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa aaccagttta
    35821 tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttcttc
    35881 atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt
    35941 tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca
    36001 gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct
    36061 gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca
    36121 ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca
    36181 gttagattgc cggataattc taagatttct ggagagtcac tttctactag tacttttgct
    36241 gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc tgcagcataa
    36301 acgaattgac cagtttttaa agcctgccca atgataggta attcattctc tacataccaa
    36361 ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg
    36421 tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt ataagcgata
    36481 taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt
    36541 cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag
    36601 tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct
    36661 ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat
    36721 tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc
    36781 actccctatt ctttttattt gttaagattt acagctgtac atttataaat tacaccttta
    36841 tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa
    36901 gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga
    36961 tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt
    37021 acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc
    37081 gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat
    37141 cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg
    37201 tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga
    37261 agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga
    37321 aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct
    37381 gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct
    37441 tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg
    37501 ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa
    37561 agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta
    37621 tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta
    37681 actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt
    37741 aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct
    37801 aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt agtgtttgga
    37861 acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg
    37921 actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg
    37981 ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta
    38041 ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac
    38101 tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga
    38161 aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct
    38221 ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa
    38281 gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc tgcatagatt
    38341 ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa
    38401 gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca
    38461 aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc aaatccataa
    38521 gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga
    38581 gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca
    38641 ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc
    38701 atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg
    38761 ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa
    38821 ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctagg
    38881 agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct
    38941 gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt
    39001 attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga
    39061 tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc aaagaaagtc
    39121 aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa
    39181 agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt
    39241 tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag cggtaaaaat
    39301 atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca
    39361 aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc tcttttatca
    39421 attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata aataaaaaac
    39481 aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat tctctaagaa
    39541 aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa tatgtaaaat
    39601 cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt tcatgaaagg
    39661 agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa cgcggttaca
    39721 gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac ttgtccgcaa
    39781 cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag accaacatga
    39841 cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct gctgtcttat
    39901 tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa ctctcgtccc
    39961 ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt cttatagaac
    40021 atccgcaatt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata aattattcag
    40081 ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac aaaaacagga
    40141 aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt gaatcgctca
    40201 ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt gctactctcg
    40261 aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag gactgttatt
    40321 atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa atgttctctt
    40381 ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac tccataaaaa
    40441 tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa atagataaaa
    40501 aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta aacgataaca
    40561 accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga atcatgcaaa
    40621 ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta ttaggtacgc
    40681 gttttgtttt ataagtcatg acaaatttcc ttcgttgctg ggtagatttt ccggcattgt
    40741 tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc atctgtatat
    40801 tggataaaat tctgacaaca actttcaggt gtgtcgaacc actctaatct ttaaagataa
    40861 atattaaaat aacgattaat aatattaaca gtctttattt ttcaccacca aaaacctatc
    40921 tttgcatata ctaaatagac gtatatacac agataggttt gtcttgtgaa aaataatgat
    40981 tatccttacc aaaaaatgtt tgtttttcta catgttcgta cttatcaaaa aatatgatcc
    41041 ttattgaaat tactggctat ttatattcca acgacttctt gtgttaattt ttgttatctc
    41101 atagtaaatt atgtttcatt ttcacttaat ttagttgtca cgtgttcgtc aattcgtaaa
    41161 aacggtaaat atataaacac acttgcggct ataattaata tttgtaaaac agctgctcgc
    41221 cagtctccgg ctgttgctaa aaatcccgat ataactggtg gcgtagtcca aggaatttgc
    41281 actactacat gattaattaa tcccacagca gttgcaaaat atgctactgt caaagaaaaa
    41341 atcggtgcaa gcacaaaggg aataatcaga ctgatattaa atacaatagg caatccaaaa
    41401 ataataggtt cgcttatatt gaatatagcg ggagttaccg ataatttagc aaagtcctta
    41461 taatcttttc tacgactgaa gataaatatt gcaatcaata aagcaatcgt atttcccgat
    41521 ccacccatta ccgcaaaggt atctttaaag gccatattga taatatgtgg aatttgctct
    41581 ttgtttgcat aagctgccat attttcttgc atattctgaa tcaaaaacgg atctaacaat
    41641 gaacctgata ttactgcttg atggatacca aatccaaaaa atagattagc aatagatgta
    41701 attaacaaaa atcctggtaa accagttgta attttactta atggctctgt aactatcgtg
    41761 gtaattagct tatttatatc catattaaac aattgaatga ttataaatga caataaagca
    41821 aacgaggtaa tcgtaataat gattgggatt aatacattaa aagactttaa aacagcggga
    41881 ggaatatttc cggatagctg aatctgtagt ttcttattat tagaaagttt aataaataat
    41941 tctgtagccg ctaatccaac aaatatacct acaaacattc ctgaagctcc tgtataggat
    42001 ataggtatca cacccgtcac ttcaacagct ttatccgcat tttcaggagt aaacatcatc
    42061 tgcataggtg ttaatacaac aaatgtcgaa atagcaacta ataccgctgc aatatgattt
    42121 gtataatttc tgcttgcaca catatgataa gatactgctg ctacaactaa taatgtagtt
    42181 atgcttaatg taccattggc aattgaagta ccaatttgtt gtaacgtggt caatgtacta
    42241 gtatcgacaa tttcccccat gaatcctgtt ggttctaaga tcactgaatt aataagtgtc
    42301 acaaatcctg ctaacatcat aaatggcata aatgtagcaa acgcatctcg caaagttctt
    42361 aaatggatct gattccccaa cttagtcgca actataccaa atttttcaaa tacaatgtcc
    42421 ttcctgctac ttttgcccat attttttctc ctttatgctt attgtaatcg attacttttt
    42481 ccacccaaat aatacatcac ttatcaaaaa
//
LOCUS       DAJSNV010000016        41582 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSNV010000016 DAJSNV010000000
VERSION     DAJSNV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.017"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..611)
                     /locus_tag="ON516_000827"
     CDS             complement(81..611)
                     /locus_tag="ON516_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3344326.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(710..1060)
                     /locus_tag="ON516_000828"
     CDS             complement(710..1060)
                     /locus_tag="ON516_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3344327.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1217..2746)
                     /gene="ahpF"
                     /locus_tag="ON516_000829"
     CDS             complement(1217..2746)
                     /gene="ahpF"
                     /locus_tag="ON516_000829"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3344328.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2756..3319)
                     /gene="ahpC"
                     /locus_tag="ON516_000830"
     CDS             complement(2756..3319)
                     /gene="ahpC"
                     /locus_tag="ON516_000830"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3344329.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3463..4125)
                     /locus_tag="ON516_000831"
     CDS             complement(3463..4125)
                     /locus_tag="ON516_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3344330.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4309..5955
                     /locus_tag="ON516_000832"
     CDS             4309..5955
                     /locus_tag="ON516_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3344331.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5946..7028
                     /gene="uxuA"
                     /locus_tag="ON516_000833"
     CDS             5946..7028
                     /gene="uxuA"
                     /locus_tag="ON516_000833"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3344332.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7136..8689)
                     /locus_tag="ON516_000834"
                     /pseudo
     CDS             complement(7136..8689)
                     /locus_tag="ON516_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8837..9978
                     /locus_tag="ON516_000835"
                     /pseudo
     CDS             8837..9978
                     /locus_tag="ON516_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10104..11183)
                     /locus_tag="ON516_000836"
     CDS             complement(10104..11183)
                     /locus_tag="ON516_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3344333.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11292..11732)
                     /locus_tag="ON516_000837"
     CDS             complement(11292..11732)
                     /locus_tag="ON516_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3344334.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11789..12199)
                     /locus_tag="ON516_000838"
     CDS             complement(11789..12199)
                     /locus_tag="ON516_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3344335.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12380..12814)
                     /locus_tag="ON516_000839"
     CDS             complement(12380..12814)
                     /locus_tag="ON516_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3344336.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(12914..13762)
                     /locus_tag="ON516_000840"
     CDS             complement(12914..13762)
                     /locus_tag="ON516_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3344337.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14151..15917
                     /locus_tag="ON516_000841"
     CDS             14151..15917
                     /locus_tag="ON516_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3344338.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16019..17278
                     /locus_tag="ON516_000842"
     CDS             16019..17278
                     /locus_tag="ON516_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3344339.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17425..18726
                     /locus_tag="ON516_000843"
     CDS             17425..18726
                     /locus_tag="ON516_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344340.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18729..19580
                     /locus_tag="ON516_000844"
     CDS             18729..19580
                     /locus_tag="ON516_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344341.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19593..20408
                     /locus_tag="ON516_000845"
     CDS             19593..20408
                     /locus_tag="ON516_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3344342.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            20559..21725
                     /locus_tag="ON516_000846"
     CDS             20559..21725
                     /locus_tag="ON516_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3344343.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            21955..22602
                     /locus_tag="ON516_000847"
     CDS             21955..22602
                     /locus_tag="ON516_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3344344.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            22686..23630
                     /locus_tag="ON516_000848"
     CDS             22686..23630
                     /locus_tag="ON516_000848"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3344345.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            23765..25216
                     /locus_tag="ON516_000849"
     CDS             23765..25216
                     /locus_tag="ON516_000849"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3344346.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            25237..26073
                     /locus_tag="ON516_000850"
     CDS             25237..26073
                     /locus_tag="ON516_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3344347.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            26073..26732
                     /locus_tag="ON516_000851"
     CDS             26073..26732
                     /locus_tag="ON516_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3344348.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            26858..27547
                     /locus_tag="ON516_000852"
     CDS             26858..27547
                     /locus_tag="ON516_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344349.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            27553..28509
                     /locus_tag="ON516_000853"
     CDS             27553..28509
                     /locus_tag="ON516_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3344350.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            28553..29065
                     /locus_tag="ON516_000854"
     CDS             28553..29065
                     /locus_tag="ON516_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344351.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(29321..29869)
                     /locus_tag="ON516_000855"
     CDS             complement(29321..29869)
                     /locus_tag="ON516_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3344352.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(29958..31139)
                     /locus_tag="ON516_000856"
     CDS             complement(29958..31139)
                     /locus_tag="ON516_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3344353.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(31375..32883)
                     /locus_tag="ON516_000857"
     CDS             complement(31375..32883)
                     /locus_tag="ON516_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3344354.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            33042..33233
                     /locus_tag="ON516_000858"
     CDS             33042..33233
                     /locus_tag="ON516_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344355.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            33436..34353
                     /locus_tag="ON516_000859"
     CDS             33436..34353
                     /locus_tag="ON516_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3344356.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            34346..35419
                     /locus_tag="ON516_000860"
     CDS             34346..35419
                     /locus_tag="ON516_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344357.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            35567..36085
                     /locus_tag="ON516_000861"
     CDS             35567..36085
                     /locus_tag="ON516_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344358.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            36066..36458
                     /locus_tag="ON516_000862"
     CDS             36066..36458
                     /locus_tag="ON516_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3344359.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            36534..37469
                     /locus_tag="ON516_000863"
     CDS             36534..37469
                     /locus_tag="ON516_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3344360.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            37602..38381
                     /locus_tag="ON516_000864"
     CDS             37602..38381
                     /locus_tag="ON516_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3344361.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            38374..38691
                     /locus_tag="ON516_000865"
     CDS             38374..38691
                     /locus_tag="ON516_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3344362.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            38935..40545
                     /locus_tag="ON516_000866"
     CDS             38935..40545
                     /locus_tag="ON516_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3344363.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            40702..41550
                     /locus_tag="ON516_000867"
     CDS             40702..41550
                     /locus_tag="ON516_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3344364.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13657 a         7471 c         7984 g        12470 t
ORIGIN      
        1 actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt tatacggtct
       61 attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat gaaattgata
      121 atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag aagcatcaaa
      181 cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat actactataa
      241 acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa gctgttgatg
      301 ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc aaaagcatag
      361 acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact ccaagaacct
      421 gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa aacaagatag
      481 gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc
      541 ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt ttgtttaatc
      601 gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag tcattttacc
      661 aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat caacttctga
      721 ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct aaataacgaa
      781 tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca acacctgtaa
      841 tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg aaagtcactt
      901 gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa caaatgattt
      961 catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac ataggataaa
     1021 cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac ctttcttttt
     1081 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
     1141 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
     1201 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
     1261 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
     1321 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
     1381 tcatttcgat cgttctatct agccaatccg tattagggag taagccgact agaatgaata
     1441 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
     1501 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
     1561 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
     1621 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
     1681 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
     1741 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
     1801 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
     1861 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
     1921 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
     1981 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
     2041 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
     2101 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
     2161 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
     2221 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
     2281 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
     2341 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
     2401 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
     2461 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
     2521 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
     2581 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
     2641 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
     2701 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
     2761 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
     2821 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
     2881 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
     2941 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
     3001 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
     3061 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
     3121 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
     3181 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc ataagatctt
     3241 tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat tcttcgattt
     3301 ttttcccaat caatgacata gtaaaacctc caataataat tatttattta taattattgt
     3361 aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga ttactcaaaa
     3421 acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct ggaaatactc
     3481 gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt
     3541 ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat cataatgttg
     3601 ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc ggaaacgttc
     3661 taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg caatttcgaa
     3721 acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac
     3781 tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt
     3841 ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc gagcattttc
     3901 tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg tatacacgag
     3961 ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga tagcaagatg
     4021 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
     4081 tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta ttcctccaaa
     4141 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
     4201 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
     4261 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
     4321 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
     4381 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
     4441 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
     4501 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
     4561 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
     4621 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
     4681 gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact
     4741 gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat
     4801 ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt gctactttat
     4861 aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa
     4921 aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt
     4981 tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg
     5041 acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa
     5101 gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt
     5161 gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg
     5221 aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa agcagatacg
     5281 atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta
     5341 ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct
     5401 gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag
     5461 atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc taatatgcct
     5521 cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa
     5581 aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc
     5641 gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt
     5701 ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact gggtacgcaa
     5761 gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt
     5821 gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta
     5881 gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat
     5941 ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg
     6001 aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag
     6061 gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt
     6121 taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt
     6181 cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa
     6241 tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact
     6301 accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc
     6361 caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg ccgggatggg
     6421 aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca gtagacgaag
     6481 aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg
     6541 taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt ggattagctc
     6601 gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac agcccttata
     6661 acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga
     6721 ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga aatattaaat
     6781 ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt tcattagaca
     6841 tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc
     6901 atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat
     6961 taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag
     7021 ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattcttg
     7081 atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat ttggtctagg
     7141 aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt aattcaaaga
     7201 tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg attcctttta
     7261 aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg ctgataacga
     7321 tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa aaaatttgtt
     7381 ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc acaccaatca
     7441 caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca accatccatt
     7501 gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct ctgaaatcca
     7561 tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct tgataaagca
     7621 tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta taaagtttat
     7681 ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac aaatcaaata
     7741 cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg tttgcttttt
     7801 ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg atagaaagtc
     7861 gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa
     7921 tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat tctttttgta
     7981 cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga aaatataatt
     8041 tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca
     8101 taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt gaaaagatgt
     8161 tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa aaagactttc
     8221 tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca ttaatctttt
     8281 tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa ctgataatcg
     8341 aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat tcgttgcagt
     8401 atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa ttcaacttta
     8461 aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca aagtcttgtt
     8521 ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga tctgctaaat
     8581 attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat
     8641 tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg aaagaggctc
     8701 ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa
     8761 aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc
     8821 aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg cagccttaag
     8881 cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag ccaaatggac
     8941 agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa
     9001 ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta
     9061 ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccac
     9121 tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac
     9181 cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc
     9241 agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga caattgagca
     9301 agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa
     9361 tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat
     9421 agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata aatcaaaacc
     9481 tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa
     9541 ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc
     9601 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
     9661 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
     9721 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
     9781 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
     9841 tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata
     9901 ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa
     9961 caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga
    10021 aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata
    10081 ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg
    10141 atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag
    10201 ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc
    10261 agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca
    10321 atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg
    10381 aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa
    10441 atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg
    10501 agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg
    10561 acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa
    10621 aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg
    10681 aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc
    10741 gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat
    10801 tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aatcaataac ggactggatc
    10861 atatcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg
    10921 taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc
    10981 tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt
    11041 gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg
    11101 cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa
    11161 caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa gagaaagagc
    11221 aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc
    11281 atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc
    11341 agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt
    11401 ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag gagtcagcgt aacatttacg
    11461 attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt
    11521 agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa
    11581 gtctttttct tttcccataa aactaacatc acaagatact gggtataggt aagattcagc
    11641 ggcttgagga gggaagtgta gtgtctaatg atttccttag aggcggcata taaaggaaaa
    11701 catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt
    11761 gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta
    11821 tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt
    11881 gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc
    11941 tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat
    12001 ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa
    12061 acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt
    12121 cgaatgtacg atcgggatta tgaacttctc cagagcgtcc gcctgtgttg atcattttcg
    12181 tagaatacat ttttttcata aaattaccac ctttaattat attttataca attgaatttt
    12241 acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca
    12301 catgaaacag acgcgctttc tcgaatagca aacatataca aaaaaatatc agagacaaaa
    12361 actaatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaac
    12421 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
    12481 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
    12541 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
    12601 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
    12661 atctcgtgcc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
    12721 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
    12781 ttaatacaaa atctatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
    12841 acagttacta gattctttta ttataccagt taaagaaaac gcattcaaaa aaatagcgga
    12901 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
    12961 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
    13021 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
    13081 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
    13141 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
    13201 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
    13261 ttccaaaaac aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
    13321 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
    13381 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
    13441 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
    13501 aaaacaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
    13561 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaat cctaaaacag
    13621 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    13681 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    13741 taaattgtct cgtttctttc atttatccac ttccttaatt cattaataat actcttatct
    13801 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    13861 aaattattat gtttcattct tctagaatat acttctacgt taaaaactgt taaaaaactt
    13921 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    13981 aggaagtaga ttaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    14041 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgttt
    14101 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    14161 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    14221 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgctt tgtggagatc
    14281 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    14341 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagg cgcttacagt
    14401 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    14461 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    14521 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    14581 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    14641 gaagtaaaga tccagaccga gaagacttct tcgggggaga tttgcaaggt gtgagggatc
    14701 atttggatta cttgacggac cttggcgtca atggcatcta tttttgtccc atatttaaag
    14761 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    14821 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    14881 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    14941 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    15001 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    15061 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    15121 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    15181 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    15241 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    15301 atgcagtgat gaattatgca ttcacagatt cgattaaaga ttattttgca aaaaagaaaa
    15361 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    15421 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    15481 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    15541 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    15601 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    15661 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    15721 taatagtata tgatcgtgaa gataagctct atttcacacg ggaactagag ggacagatca
    15781 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    15841 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    15901 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    15961 tttgtgccca ttataataag ggtgtaacag cacgttgaat acacaggagg aaatgaagat
    16021 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    16081 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    16141 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    16201 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    16261 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    16321 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    16381 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    16441 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    16501 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    16561 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    16621 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    16681 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    16741 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    16801 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    16861 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    16921 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    16981 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    17041 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    17101 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    17161 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    17221 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    17281 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    17341 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    17401 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    17461 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    17521 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    17581 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    17641 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    17701 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    17761 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    17821 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    17881 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    17941 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    18001 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    18061 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    18121 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    18181 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    18241 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    18301 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    18361 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    18421 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    18481 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggtggt
    18541 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    18601 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    18661 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    18721 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    18781 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    18841 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    18901 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    18961 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    19021 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    19081 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    19141 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    19201 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    19261 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    19321 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    19381 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    19441 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    19501 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    19561 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    19621 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    19681 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    19741 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    19801 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    19861 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    19921 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    19981 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    20041 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    20101 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    20161 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    20221 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    20281 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    20341 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    20401 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    20461 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    20521 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    20581 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    20641 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    20701 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    20761 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    20821 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    20881 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    20941 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    21001 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    21061 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    21121 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    21181 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    21241 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    21301 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    21361 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    21421 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    21481 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    21541 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    21601 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    21661 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    21721 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    21781 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    21841 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    21901 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    21961 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    22021 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    22081 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    22141 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    22201 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    22261 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    22321 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    22381 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    22441 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    22501 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    22561 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    22621 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    22681 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    22741 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    22801 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    22861 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    22921 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    22981 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    23041 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    23101 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    23161 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    23221 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    23281 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    23341 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    23401 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    23461 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtacagag cttcataaag
    23521 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    23581 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    23641 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    23701 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    23761 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    23821 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    23881 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    23941 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    24001 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    24061 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    24121 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    24181 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    24241 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    24301 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    24361 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    24421 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    24481 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    24541 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    24601 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    24661 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    24721 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    24781 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    24841 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    24901 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    24961 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    25021 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    25081 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    25141 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    25201 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    25261 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    25321 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    25381 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    25441 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    25501 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    25561 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    25621 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    25681 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    25741 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    25801 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    25861 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    25921 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    25981 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    26041 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcccctaatt gatttttccc
    26101 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    26161 tcgtgtcgtt tcttttagga aatttggtag ggttgctttt gacagtttta ggtcttttgg
    26221 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    26281 cactagtcct gctgtttctg ttatatttcg ggttacctta tcaattaagt gccttaacgg
    26341 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    26401 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    26461 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    26521 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    26581 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    26641 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    26701 tagaaaaacg ttctatccat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    26761 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    26821 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    26881 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    26941 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    27001 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    27061 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    27121 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    27181 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    27241 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    27301 aaaagcaaag caaatggagc gaggcgtaaa atattttcca aaagatcaat tgtacaaaga
    27361 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    27421 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    27481 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    27541 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    27601 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    27661 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
    27721 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    27781 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    27841 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    27901 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    27961 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    28021 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    28081 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    28141 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    28201 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    28261 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    28321 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    28381 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    28441 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    28501 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    28561 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    28621 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    28681 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    28741 tggaacagct gcattagtct caggcgtgga tcccgtttac acaaaaatag atggtagttg
    28801 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    28861 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    28921 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    28981 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    29041 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    29101 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    29161 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    29221 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    29281 acgatctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    29341 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    29401 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    29461 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    29521 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    29581 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    29641 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    29701 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    29761 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    29821 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    29881 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    29941 ctttgctttt ttgtatttta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    30001 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    30061 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    30121 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    30181 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    30241 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    30301 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    30361 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    30421 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    30481 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    30541 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    30601 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    30661 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    30721 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
    30781 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    30841 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    30901 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    30961 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    31021 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    31081 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    31141 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    31201 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    31261 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    31321 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    31381 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    31441 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    31501 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    31561 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    31621 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    31681 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    31741 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    31801 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    31861 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    31921 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    31981 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    32041 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    32101 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    32161 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    32221 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    32281 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaagcaag tagcgagctt
    32341 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    32401 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    32461 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    32521 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    32581 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    32641 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    32701 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    32761 cttgtcgtca actaacttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    32821 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    32881 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    32941 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    33001 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    33061 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    33121 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    33181 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    33241 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    33301 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    33361 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    33421 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    33481 gaggagacga acgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    33541 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    33601 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    33661 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    33721 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    33781 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    33841 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    33901 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    33961 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    34021 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    34081 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    34141 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    34201 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    34261 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    34321 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    34381 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    34441 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac atgaaaaaga
    34501 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    34561 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    34621 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    34681 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    34741 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    34801 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    34861 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    34921 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    34981 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    35041 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    35101 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    35161 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    35221 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    35281 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    35341 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    35401 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    35461 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    35521 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    35581 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    35641 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    35701 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    35761 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    35821 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    35881 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    35941 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    36001 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    36061 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    36121 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    36181 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgg
    36241 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    36301 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    36361 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    36421 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    36481 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    36541 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    36601 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    36661 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    36721 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    36781 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    36841 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    36901 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    36961 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    37021 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    37081 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    37141 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    37201 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    37261 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    37321 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    37381 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    37441 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    37501 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    37561 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    37621 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    37681 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    37741 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    37801 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    37861 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    37921 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    37981 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    38041 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    38101 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    38161 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    38221 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    38281 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    38341 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    38401 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    38461 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    38521 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    38581 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    38641 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    38701 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    38761 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    38821 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    38881 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    38941 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    39001 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    39061 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    39121 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    39181 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    39241 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    39301 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    39361 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    39421 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    39481 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    39541 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    39601 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    39661 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    39721 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    39781 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    39841 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    39901 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    39961 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    40021 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    40081 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    40141 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    40201 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    40261 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    40321 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    40381 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    40441 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    40501 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    40561 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    40621 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    40681 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    40741 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    40801 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    40861 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    40921 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    40981 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    41041 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    41101 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    41161 aatacctatg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    41221 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    41281 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    41341 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    41401 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    41461 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    41521 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    41581 at
//
LOCUS       DAJSNV010000017        36551 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSNV010000017 DAJSNV010000000
VERSION     DAJSNV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.018"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..695
                     /locus_tag="ON516_000868"
     CDS             24..695
                     /locus_tag="ON516_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3344365.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="ON516_000869"
     CDS             754..1194
                     /locus_tag="ON516_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344366.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="ON516_000870"
     CDS             1206..1874
                     /locus_tag="ON516_000870"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3344367.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="ON516_000871"
     CDS             1938..2561
                     /locus_tag="ON516_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3344368.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="ON516_000872"
     CDS             2617..3003
                     /locus_tag="ON516_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3344369.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="ON516_000873"
     CDS             3216..4607
                     /locus_tag="ON516_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3344370.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5218..5586
                     /locus_tag="ON516_000874"
     CDS             5218..5586
                     /locus_tag="ON516_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3344371.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5766..6707
                     /locus_tag="ON516_000875"
     CDS             5766..6707
                     /locus_tag="ON516_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3344372.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6776..7507
                     /locus_tag="ON516_000876"
     CDS             6776..7507
                     /locus_tag="ON516_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3344373.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7651..9831
                     /locus_tag="ON516_000877"
     CDS             7651..9831
                     /locus_tag="ON516_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3344374.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9851..10333
                     /locus_tag="ON516_000878"
     CDS             9851..10333
                     /locus_tag="ON516_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3344375.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10349..11110
                     /locus_tag="ON516_000879"
     CDS             10349..11110
                     /locus_tag="ON516_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3344376.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11241..12575
                     /locus_tag="ON516_000880"
     CDS             11241..12575
                     /locus_tag="ON516_000880"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3344377.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12882..13367
                     /locus_tag="ON516_000881"
     CDS             12882..13367
                     /locus_tag="ON516_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3344378.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13360..14043
                     /locus_tag="ON516_000882"
     CDS             13360..14043
                     /locus_tag="ON516_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3344379.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14169..16814
                     /gene="polA"
                     /locus_tag="ON516_000883"
     CDS             14169..16814
                     /gene="polA"
                     /locus_tag="ON516_000883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3344380.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16859..17695
                     /gene="mutM"
                     /locus_tag="ON516_000884"
     CDS             16859..17695
                     /gene="mutM"
                     /locus_tag="ON516_000884"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3344381.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17659..18288
                     /locus_tag="ON516_000885"
     CDS             17659..18288
                     /locus_tag="ON516_000885"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3344382.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18340..18672
                     /locus_tag="ON516_000886"
     CDS             18340..18672
                     /locus_tag="ON516_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3344383.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18984..19475
                     /gene="nrdR"
                     /locus_tag="ON516_000887"
     CDS             18984..19475
                     /gene="nrdR"
                     /locus_tag="ON516_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3344384.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19498..20871
                     /locus_tag="ON516_000888"
     CDS             19498..20871
                     /locus_tag="ON516_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3344385.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20871..21797
                     /gene="dnaI"
                     /locus_tag="ON516_000889"
     CDS             20871..21797
                     /gene="dnaI"
                     /locus_tag="ON516_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3344386.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21926..22366
                     /locus_tag="ON516_000890"
     CDS             21926..22366
                     /locus_tag="ON516_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3344387.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22510..23211
                     /locus_tag="ON516_000891"
     CDS             22510..23211
                     /locus_tag="ON516_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3344388.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23208..24329
                     /locus_tag="ON516_000892"
     CDS             23208..24329
                     /locus_tag="ON516_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3344389.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24344..25576
                     /gene="pepT"
                     /locus_tag="ON516_000893"
     CDS             24344..25576
                     /gene="pepT"
                     /locus_tag="ON516_000893"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3344390.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25708..26616
                     /locus_tag="ON516_000894"
     CDS             25708..26616
                     /locus_tag="ON516_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3344391.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26651..26923
                     /locus_tag="ON516_000895"
     CDS             26651..26923
                     /locus_tag="ON516_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3344392.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26934..27980
                     /locus_tag="ON516_000896"
     CDS             26934..27980
                     /locus_tag="ON516_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3344393.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28231..30840
                     /gene="clpB"
                     /locus_tag="ON516_000897"
     CDS             28231..30840
                     /gene="clpB"
                     /locus_tag="ON516_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3344394.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30991..31677)
                     /locus_tag="ON516_000898"
     CDS             complement(30991..31677)
                     /locus_tag="ON516_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344395.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31655..32149)
                     /locus_tag="ON516_000899"
     CDS             complement(31655..32149)
                     /locus_tag="ON516_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3344396.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32169..33389)
                     /locus_tag="ON516_000900"
     CDS             complement(32169..33389)
                     /locus_tag="ON516_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344397.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33536..35371
                     /gene="lepA"
                     /locus_tag="ON516_000901"
     CDS             33536..35371
                     /gene="lepA"
                     /locus_tag="ON516_000901"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3344398.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35465..>36551)
                     /locus_tag="ON516_000902"
     CDS             complement(35465..>36551)
                     /locus_tag="ON516_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3344399.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12165 a         6464 c         7816 g        10106 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg
     5221 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
     5281 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
     5341 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
     5401 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
     5461 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
     5521 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
     5581 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
     5641 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
     5701 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
     5761 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
     5821 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
     5881 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
     5941 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
     6001 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
     6061 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
     6121 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
     6181 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     6241 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     6301 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     6361 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     6421 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     6481 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     6541 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     6601 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     6661 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     6721 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     6781 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     6841 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     6901 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     6961 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     7021 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     7081 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     7141 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     7201 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     7261 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     7321 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     7381 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     7441 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     7501 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     7561 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     7621 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     7681 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     7741 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     7801 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     7861 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     7921 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     7981 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     8041 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     8101 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     8161 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     8221 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     8281 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     8341 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     8401 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     8461 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     8521 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     8581 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     8641 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     8701 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     8761 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     8821 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     8881 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     8941 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     9001 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     9061 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     9121 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     9181 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     9241 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     9301 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     9361 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     9421 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     9481 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     9541 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     9601 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     9661 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     9721 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     9781 acgaaaaaag gtagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     9841 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     9901 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     9961 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
    10021 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
    10081 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
    10141 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
    10201 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
    10261 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
    10321 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
    10381 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
    10441 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
    10501 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
    10561 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
    10621 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
    10681 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
    10741 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
    10801 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
    10861 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
    10921 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
    10981 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
    11041 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
    11101 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
    11161 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
    11221 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
    11281 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
    11341 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
    11401 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
    11461 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
    11521 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
    11581 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
    11641 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
    11701 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
    11761 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
    11821 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
    11881 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
    11941 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
    12001 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
    12061 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
    12121 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
    12181 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
    12241 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
    12301 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
    12361 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
    12421 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
    12481 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
    12541 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
    12601 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
    12661 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
    12721 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
    12781 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
    12841 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
    12901 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
    12961 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
    13021 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
    13081 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
    13141 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
    13201 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
    13261 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
    13321 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca
    13381 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
    13441 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
    13501 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
    13561 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
    13621 tctcttttga atggcataac attagcggtt actcttgcat actacgatat tggaacagta
    13681 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
    13741 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
    13801 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
    13861 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
    13921 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
    13981 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
    14041 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
    14101 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
    14161 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
    14221 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
    14281 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
    14341 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
    14401 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
    14461 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
    14521 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
    14581 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
    14641 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
    14701 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
    14761 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
    14821 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
    14881 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
    14941 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
    15001 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
    15061 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
    15121 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg
    15181 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    15241 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    15301 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    15361 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    15421 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    15481 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    15541 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    15601 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    15661 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    15721 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    15781 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    15841 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    15901 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    15961 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    16021 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    16081 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    16141 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    16201 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    16261 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    16321 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    16381 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    16441 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    16501 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    16561 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    16621 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    16681 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    16741 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    16801 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    16861 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    16921 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    16981 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    17041 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    17101 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    17161 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    17221 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    17281 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    17341 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    17401 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    17461 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    17521 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    17581 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    17641 acaaaggggg acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt
    17701 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    17761 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    17821 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    17881 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    17941 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    18001 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    18061 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    18121 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    18181 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaaaa
    18241 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    18301 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    18361 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    18421 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    18481 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    18541 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    18601 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    18661 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    18721 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    18781 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    18841 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    18901 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    18961 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    19021 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    19081 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    19141 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    19201 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    19261 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    19321 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    19381 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    19441 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    19501 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    19561 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    19621 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    19681 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    19741 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    19801 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    19861 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    19921 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    19981 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    20041 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    20101 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    20161 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    20221 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    20281 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    20341 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    20401 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    20461 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    20521 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    20581 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    20641 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    20701 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    20761 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    20821 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    20881 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    20941 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    21001 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    21061 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    21121 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    21181 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    21241 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    21301 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    21361 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    21421 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    21481 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    21541 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    21601 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    21661 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    21721 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    21781 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    21841 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    21901 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    21961 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    22021 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    22081 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    22141 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    22201 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    22261 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    22321 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    22381 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    22441 gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg
    22501 agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc
    22561 cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg
    22621 atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa
    22681 tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca
    22741 aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg
    22801 ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga
    22861 caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa
    22921 gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa
    22981 atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc
    23041 gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt
    23101 caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa
    23161 aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg
    23221 gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag
    23281 acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat
    23341 tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg
    23401 ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa
    23461 aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg
    23521 atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga
    23581 gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg
    23641 atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt
    23701 ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac
    23761 aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta
    23821 ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg
    23881 aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac
    23941 gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag
    24001 cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc
    24061 gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag
    24121 cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg
    24181 ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg
    24241 agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa
    24301 caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt
    24361 gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac
    24421 tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt
    24481 aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag
    24541 caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt
    24601 taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt
    24661 actagatgcg gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta
    24721 tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc
    24781 agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca
    24841 cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt
    24901 cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga
    24961 gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt
    25021 acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca
    25081 taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca
    25141 tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca
    25201 tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa
    25261 tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa
    25321 agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct
    25381 tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta
    25441 catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga
    25501 atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt
    25561 gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat
    25621 ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt
    25681 ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt
    25741 tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag
    25801 cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta
    25861 gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg
    25921 attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt
    25981 ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa
    26041 gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat
    26101 gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt
    26161 caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg
    26221 ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa
    26281 tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac
    26341 tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc
    26401 cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt
    26461 acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga
    26521 acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta
    26581 acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg
    26641 aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa
    26701 tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg
    26761 tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat
    26821 ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc
    26881 aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt
    26941 ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca
    27001 ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca
    27061 gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc
    27121 gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct
    27181 tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct
    27241 cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag
    27301 cactccatat gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca
    27361 tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta
    27421 tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc
    27481 aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg
    27541 tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag
    27601 acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg
    27661 tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt
    27721 cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt
    27781 cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt
    27841 cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt
    27901 atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga
    27961 ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa
    28021 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
    28081 attttgtttt tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat
    28141 tttgaccaaa attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga
    28201 tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt
    28261 caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac
    28321 attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg
    28381 gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat
    28441 ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga
    28501 ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa
    28561 gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa
    28621 aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga
    28681 gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta
    28741 gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt
    28801 gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc
    28861 ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg
    28921 cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga
    28981 gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa
    29041 agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa
    29101 acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta
    29161 catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg
    29221 ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt
    29281 attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac
    29341 gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac
    29401 aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg
    29461 ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca
    29521 ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta
    29581 gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa
    29641 gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat
    29701 gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa
    29761 ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt
    29821 caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc
    29881 gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat
    29941 aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca
    30001 cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact
    30061 ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat
    30121 catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc
    30181 ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga
    30241 cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc
    30301 aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta
    30361 gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa
    30421 ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta
    30481 cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca
    30541 ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt
    30601 ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa
    30661 aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta
    30721 gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg
    30781 attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa
    30841 tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt
    30901 ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga
    30961 agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata
    31021 actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc
    31081 agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac
    31141 aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca
    31201 taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa
    31261 gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa
    31321 tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa
    31381 agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa
    31441 ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg
    31501 tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga
    31561 agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa
    31621 agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta
    31681 aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt
    31741 tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta
    31801 gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga
    31861 tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg
    31921 tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa
    31981 gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag
    32041 gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat
    32101 ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat
    32161 cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt
    32221 gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg
    32281 cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata
    32341 ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta
    32401 ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac
    32461 ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc
    32521 tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct
    32581 ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga
    32641 gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag
    32701 aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct
    32761 tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg
    32821 ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc
    32881 tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa
    32941 agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac
    33001 aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct
    33061 tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac accacccatt
    33121 ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt
    33181 ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg
    33241 ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac
    33301 acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca
    33361 agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca
    33421 gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt
    33481 actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga
    33541 tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat
    33601 cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc
    33661 tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac
    33721 gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt
    33781 tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc
    33841 tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt
    33901 ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat
    33961 cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat
    34021 cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct
    34081 cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc
    34141 tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac
    34201 ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga
    34261 tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg
    34321 tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac
    34381 agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc
    34441 gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc
    34501 tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc
    34561 attaggtttt gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga
    34621 acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca
    34681 tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc
    34741 tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga
    34801 ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga
    34861 ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt
    34921 tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat
    34981 gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt
    35041 cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt
    35101 tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat
    35161 tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa
    35221 atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa
    35281 gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct
    35341 gaaaatggac gaggacgaac cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa
    35401 ataaaccatg tttacatgga tttgccaccg gtttgccacc ggtggttttt ttcggtggca
    35461 aaaatcaaga attctttcta agattttcca tgatatcgac cgtttcattt ctcatcttat
    35521 ctgttacatg cgagtaagta tccatcgtaa ttgaaattct gctgtggccc aatcgttcag
    35581 agatttcttt catttttgcg ccattttcga gaagaagtgt agcatgagta tgtctgagag
    35641 aatggaagtt aaaagagagg gagagtgcat tcgatatcct cctagtattc catttcacta
    35701 cacttggcgt aactagctca ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga
    35761 gctttccata cttcattcga ttttctaatt gttgtttctt atgttttttc agaattgcta
    35821 gcaaggtttg tccaataaaa atcgttcgat tggaactgct tgtctttggc gtaccatata
    35881 cccatgcgcc gtcattcttt accatttgtt tctctacagt aattgttcca tttgaaaaat
    35941 cgacatcatc ccacgtcaga ccacaaactt cgccaacgcg cattcccgta taaaatccaa
    36001 tattcaaagg aatatagaaa ggatgtcctt caggagtaat ttctagcata tgatcaaagt
    36061 cctcaagaga aatgattttt agatcttttt tagtcgttgg tcgtgcttca tattttggta
    36121 tttttacata cagcatagga ttttgcttga ttaatcccca aggataaacc gccatattca
    36181 gcgcattctt aaggacagag tgagtaatag tcattgtttt cttcgagtaa ccctttttaa
    36241 attcagcatt gatgaaattt tgtaaaagag caggggagag atccgtaagt tttttctttc
    36301 ctaaataacc gtttatatga tttttgatgg taaatcggta gttttcatag gtattgtatt
    36361 ttagatttag tttaacgtat tcctccatcc aaaaatcaag gtattgtttt actcgagtat
    36421 ccgtacctaa aaagtattgt cctgtttcgt caatatctga taaaactttt cgtaaagcag
    36481 cttcggcctc tggtcgggtg tctccgccaa ctttctccac tttttttctt gagccatcat
    36541 cattgatatc t
//
LOCUS       DAJSNV010000018        34760 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSNV010000018 DAJSNV010000000
VERSION     DAJSNV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.019"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            364..522
                     /locus_tag="ON516_000903"
     CDS             364..522
                     /locus_tag="ON516_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344400.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            594..1820
                     /locus_tag="ON516_000904"
     CDS             594..1820
                     /locus_tag="ON516_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3344401.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1908..2300)
                     /gene="rpsI"
                     /locus_tag="ON516_000905"
     CDS             complement(1908..2300)
                     /gene="rpsI"
                     /locus_tag="ON516_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3344402.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2316..2759)
                     /gene="rplM"
                     /locus_tag="ON516_000906"
     CDS             complement(2316..2759)
                     /gene="rplM"
                     /locus_tag="ON516_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3344403.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3278..3745)
                     /locus_tag="ON516_000907"
     CDS             complement(3278..3745)
                     /locus_tag="ON516_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3344404.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3865..5403)
                     /locus_tag="ON516_000908"
     CDS             complement(3865..5403)
                     /locus_tag="ON516_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3344405.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5483..6169
                     /locus_tag="ON516_000909"
     CDS             5483..6169
                     /locus_tag="ON516_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3344406.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6370..6897)
                     /gene="msrA"
                     /locus_tag="ON516_000910"
     CDS             complement(6370..6897)
                     /gene="msrA"
                     /locus_tag="ON516_000910"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3344407.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6981..7658)
                     /gene="pgmB"
                     /locus_tag="ON516_000911"
     CDS             complement(6981..7658)
                     /gene="pgmB"
                     /locus_tag="ON516_000911"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3344408.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7655..8785)
                     /locus_tag="ON516_000912"
     CDS             complement(7655..8785)
                     /locus_tag="ON516_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3344409.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8785..10968)
                     /locus_tag="ON516_000913"
     CDS             complement(8785..10968)
                     /locus_tag="ON516_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3344410.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10965..11990)
                     /locus_tag="ON516_000914"
     CDS             complement(10965..11990)
                     /locus_tag="ON516_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3344411.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12035..12862)
                     /locus_tag="ON516_000915"
     CDS             complement(12035..12862)
                     /locus_tag="ON516_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3344412.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12859..13926)
                     /locus_tag="ON516_000916"
     CDS             complement(12859..13926)
                     /locus_tag="ON516_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3344413.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13952..14788)
                     /locus_tag="ON516_000917"
     CDS             complement(13952..14788)
                     /locus_tag="ON516_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3344414.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14785..15654)
                     /locus_tag="ON516_000918"
     CDS             complement(14785..15654)
                     /locus_tag="ON516_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344415.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15724..17007)
                     /locus_tag="ON516_000919"
     CDS             complement(15724..17007)
                     /locus_tag="ON516_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3344416.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17021..18706)
                     /locus_tag="ON516_000920"
     CDS             complement(17021..18706)
                     /locus_tag="ON516_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3344417.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18981..20006
                     /locus_tag="ON516_000921"
     CDS             18981..20006
                     /locus_tag="ON516_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3344418.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20601..24275)
                     /gene="rpoC"
                     /locus_tag="ON516_000922"
     CDS             complement(20601..24275)
                     /gene="rpoC"
                     /locus_tag="ON516_000922"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3344419.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24333..27950)
                     /gene="rpoB"
                     /locus_tag="ON516_000923"
     CDS             complement(24333..27950)
                     /gene="rpoB"
                     /locus_tag="ON516_000923"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3344420.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28678..29646
                     /locus_tag="ON516_000924"
     CDS             28678..29646
                     /locus_tag="ON516_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3344421.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29664..30569
                     /locus_tag="ON516_000925"
     CDS             29664..30569
                     /locus_tag="ON516_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344422.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30566..31372
                     /locus_tag="ON516_000926"
     CDS             30566..31372
                     /locus_tag="ON516_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3344423.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31508..32497
                     /locus_tag="ON516_000927"
     CDS             31508..32497
                     /locus_tag="ON516_000927"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3344424.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32814..33338)
                     /locus_tag="ON516_000928"
     CDS             complement(32814..33338)
                     /locus_tag="ON516_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3344425.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33625..>34760
                     /gene="scm"
                     /locus_tag="ON516_000929"
     CDS             33625..>34760
                     /gene="scm"
                     /locus_tag="ON516_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3344426.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSD"
BASE COUNT         9954 a         7359 c         6500 g        10947 t
ORIGIN      
        1 atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat
       61 taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca atttttgaat
      121 aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat
      181 acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat
      241 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
      301 aagcaattca aaaatttcat aactactatt ttaggagatg attttataga atccagagtc
      361 tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac tctagataag
      421 tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg ctttgacgta
      481 gaagatatta aagactttat gaatacattt aaaataactt gaaattttct tctcgccgca
      541 tatactaaaa ttagtgaaat atactgcctt acttttttta aggagtgagg attttgccta
      601 caaagctttc aaatgggaaa tataaaacta atctgagata tccaaaaagg tttaaagaaa
      661 taacaggaat tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa ttggcgatta
      721 aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt gagagaggag aatgcaaact
      781 cattagaatt aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa tggcttccta
      841 gatatgaatt aggtcaaaca attagatcaa acaggcctcc tagtgatata accattagta
      901 atacaaaaga tatatttcgg ttgcatattt tacctatgtt tggtgaatac gcaatgaatt
      961 atctaaatat caatactgag ataatatcag atgaattaac caaaaaatcg aaagaatatg
     1021 caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca gaaatactaa
     1081 attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca cctaaaaaaa
     1141 atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca aaagaactta
     1201 ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact tttcatgatt
     1261 acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact tacgctttgc
     1321 aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact ttagataaat
     1381 atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta ccagaagttg
     1441 taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg aaattaaaaa
     1501 ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga gaagtaaatt
     1561 gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga aggcatcctg
     1621 acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata gctagacaag
     1681 gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt tctacaacaa
     1741 agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct tttcagagag
     1801 gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca aaagaagcac
     1861 tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta acgttttgag
     1921 aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac catacgtgcg
     1981 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     2041 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     2101 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     2161 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     2221 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     2281 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     2341 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     2401 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     2461 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     2521 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct
     2581 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     2641 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     2701 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     2761 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     2821 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     2881 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     2941 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     3001 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     3061 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
     3121 tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     3181 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt
     3241 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
     3301 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
     3361 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
     3421 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
     3481 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
     3541 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
     3601 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
     3661 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
     3721 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
     3781 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
     3841 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
     3901 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
     3961 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
     4021 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
     4081 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
     4141 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
     4201 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     4261 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     4321 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     4381 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     4441 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     4501 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     4561 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     4621 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     4681 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     4741 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     4801 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     4861 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa
     4921 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     4981 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     5041 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     5101 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     5161 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     5221 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     5281 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     5341 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     5401 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     5461 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     5521 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     5581 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     5641 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     5701 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     5761 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     5821 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     5881 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     5941 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     6001 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     6061 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     6121 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     6181 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     6241 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     6301 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     6361 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     6421 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     6481 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
     6541 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
     6601 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
     6661 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
     6721 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
     6781 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
     6841 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
     6901 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
     6961 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
     7021 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
     7081 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
     7141 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
     7201 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
     7261 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
     7321 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
     7381 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
     7441 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
     7501 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
     7561 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
     7621 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
     7681 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
     7741 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
     7801 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
     7861 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
     7921 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta
     7981 aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg
     8041 cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca
     8101 tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt
     8161 gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa
     8221 tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa
     8281 ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat
     8341 aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga
     8401 tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg
     8461 tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg
     8521 ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa
     8581 atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa
     8641 ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt
     8701 cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag
     8761 aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc
     8821 cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat
     8881 tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa
     8941 agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac
     9001 tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg
     9061 aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa
     9121 taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt
     9181 actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat
     9241 tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct
     9301 gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg
     9361 acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg
     9421 aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc
     9481 tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc
     9541 cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac
     9601 tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt
     9661 ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac
     9721 atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc
     9781 aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg
     9841 accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt
     9901 ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg
     9961 aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt
    10021 ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt
    10081 cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc
    10141 ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa
    10201 aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt
    10261 ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct
    10321 gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt
    10381 attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact
    10441 gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt
    10501 ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt
    10561 gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat
    10621 gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat
    10681 ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc
    10741 accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc
    10801 tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata
    10861 gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc
    10921 atacttattg atatactcta aatcaaactg tttttcttct actttcataa ggtaataact
    10981 tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc
    11041 cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata
    11101 cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct
    11161 acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc
    11221 acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg
    11281 ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc
    11341 catttcccga atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga
    11401 acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca
    11461 taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga
    11521 acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga
    11581 tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc
    11641 tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt
    11701 ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga
    11761 aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta
    11821 actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg
    11881 actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca
    11941 tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc
    12001 ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat
    12061 gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt
    12121 cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta
    12181 gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat
    12241 ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat
    12301 cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag
    12361 agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca
    12421 aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg
    12481 attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc
    12541 gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta
    12601 gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt
    12661 caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga
    12721 ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt
    12781 caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt
    12841 gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact
    12901 ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac
    12961 aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt
    13021 gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg
    13081 agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg
    13141 aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga
    13201 agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc
    13261 tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc
    13321 accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt
    13381 agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat
    13441 tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc
    13501 aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata
    13561 attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc
    13621 tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt
    13681 tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa
    13741 taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac
    13801 aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc
    13861 gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt
    13921 tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat
    13981 cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca
    14041 atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac
    14101 cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat
    14161 tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta
    14221 taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa
    14281 tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt
    14341 gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga
    14401 ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag
    14461 cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc
    14521 caatcactac tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca
    14581 tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt
    14641 tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca
    14701 gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata
    14761 agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac
    14821 gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt
    14881 gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag
    14941 atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag
    15001 tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc
    15061 catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat
    15121 aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac
    15181 tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc
    15241 attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga
    15301 tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg
    15361 gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt
    15421 gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata
    15481 attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact
    15541 gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg
    15601 tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac
    15661 ctccccctga taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc
    15721 ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac
    15781 agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt
    15841 gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc
    15901 agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac
    15961 tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt
    16021 tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag
    16081 accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg
    16141 gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt
    16201 tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc
    16261 attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat
    16321 cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa
    16381 agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc
    16441 atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt
    16501 ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac
    16561 ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt
    16621 ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg
    16681 atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc
    16741 tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac
    16801 cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt
    16861 gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg
    16921 tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag
    16981 acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc
    17041 acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc
    17101 cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa
    17161 aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat
    17221 ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa
    17281 tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact
    17341 ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt
    17401 tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca
    17461 cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg
    17521 tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg
    17581 tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc agctgttcca
    17641 ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt
    17701 catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta
    17761 accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta
    17821 gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat
    17881 ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga
    17941 ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta
    18001 gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt
    18061 aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta
    18121 cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg
    18181 ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca
    18241 aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac
    18301 tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt
    18361 cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga
    18421 aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg
    18481 ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa
    18541 attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct
    18601 ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag
    18661 acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc
    18721 ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc
    18781 atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata
    18841 attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt
    18901 caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca
    18961 aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg
    19021 tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa
    19081 aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag
    19141 gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga
    19201 cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa
    19261 ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg
    19321 cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg
    19381 aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg
    19441 gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt
    19501 tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag
    19561 aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc
    19621 tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag
    19681 caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat
    19741 taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc
    19801 tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc
    19861 ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa
    19921 tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag
    19981 attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat
    20041 aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt
    20101 tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt
    20161 gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt
    20221 tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta tttggggata
    20281 cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg
    20341 aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt
    20401 attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg
    20461 atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc
    20521 ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca
    20581 caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt
    20641 tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt
    20701 tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc tataataaca
    20761 ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca
    20821 cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt
    20881 gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg
    20941 tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca
    21001 cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg
    21061 tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt
    21121 tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc
    21181 aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga
    21241 acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca
    21301 ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc
    21361 ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg
    21421 gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat
    21481 tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg
    21541 cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt
    21601 gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg
    21661 tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt
    21721 cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt
    21781 tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca
    21841 accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct
    21901 ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc
    21961 gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg
    22021 gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt
    22081 ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc
    22141 caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt
    22201 tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt
    22261 acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag
    22321 tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct
    22381 gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata
    22441 tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag
    22501 ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg
    22561 atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca
    22621 cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat
    22681 gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct
    22741 tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac aactggttta
    22801 ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt
    22861 gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa
    22921 gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat
    22981 gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg
    23041 attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca
    23101 tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat
    23161 aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca
    23221 acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga
    23281 ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta
    23341 actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt
    23401 ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta
    23461 ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct
    23521 tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc
    23581 cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca
    23641 cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca
    23701 tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat
    23761 tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc
    23821 tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc
    23881 aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac
    23941 cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc
    24001 gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg
    24061 tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac
    24121 ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg
    24181 taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta
    24241 tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg
    24301 atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca
    24361 aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat
    24421 tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg
    24481 tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg
    24541 tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt
    24601 tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca
    24661 tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc
    24721 catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt
    24781 tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga
    24841 gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta
    24901 cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc
    24961 catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga
    25021 cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc
    25081 cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct
    25141 tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct
    25201 tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac
    25261 aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca
    25321 tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc
    25381 aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc
    25441 aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt
    25501 aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta
    25561 tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc
    25621 aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca
    25681 actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga
    25741 gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt
    25801 ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg
    25861 cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa
    25921 gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga
    25981 ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag
    26041 tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat
    26101 acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc
    26161 ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg
    26221 atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt
    26281 tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa
    26341 ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata
    26401 cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat
    26461 ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt
    26521 tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact
    26581 ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt
    26641 tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca
    26701 ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct
    26761 tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt
    26821 acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt
    26881 gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga
    26941 tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg
    27001 tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt
    27061 tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa
    27121 cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta
    27181 cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa
    27241 ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt
    27301 aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca
    27361 cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct
    27421 tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat
    27481 ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac
    27541 tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat
    27601 gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa
    27661 cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact
    27721 gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag
    27781 tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg
    27841 taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg
    27901 aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc
    27961 cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat
    28021 tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag
    28081 aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg
    28141 aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata
    28201 ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca
    28261 ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc
    28321 tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa
    28381 catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt
    28441 ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag
    28501 aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa
    28561 tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca
    28621 tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg
    28681 aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca
    28741 caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt
    28801 gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa
    28861 gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac
    28921 aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc
    28981 gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca
    29041 gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat
    29101 gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc
    29161 gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc
    29221 caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca
    29281 gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga
    29341 atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca
    29401 aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta
    29461 gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat ggcagcaaaa
    29521 atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg
    29581 tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct
    29641 gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat
    29701 cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg
    29761 acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa
    29821 gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg
    29881 cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag
    29941 ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca
    30001 tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag
    30061 acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt
    30121 tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga
    30181 gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg
    30241 gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct gatttaaatt
    30301 ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac
    30361 gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt
    30421 ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg
    30481 ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc
    30541 aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca
    30601 tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt
    30661 agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct
    30721 gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa
    30781 tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc
    30841 taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg
    30901 aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga
    30961 ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct
    31021 gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact
    31081 gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga
    31141 attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat
    31201 ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt
    31261 agatatccag caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa
    31321 acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag
    31381 aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt
    31441 tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat
    31501 agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt
    31561 gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg
    31621 gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg
    31681 gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga
    31741 gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat
    31801 gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga
    31861 tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg
    31921 aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac
    31981 gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc
    32041 gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga
    32101 agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa
    32161 tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt
    32221 tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt
    32281 gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt
    32341 attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga
    32401 aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc
    32461 tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca
    32521 cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat
    32581 cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg
    32641 tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa
    32701 aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa
    32761 acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct
    32821 cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca
    32881 ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact
    32941 tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct
    33001 cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga
    33061 ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct
    33121 tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga
    33181 gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga
    33241 aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc
    33301 cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc tttttattta
    33361 tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt
    33421 ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt
    33481 cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa
    33541 acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg
    33601 tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct
    33661 ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa
    33721 gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt
    33781 caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata
    33841 gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg
    33901 aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct
    33961 acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa
    34021 gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat
    34081 aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta
    34141 gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca
    34201 gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta
    34261 aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat
    34321 caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc
    34381 aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg
    34441 aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt
    34501 gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat
    34561 gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat
    34621 ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca
    34681 gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat
    34741 acatcagctt caagcgacag
//
LOCUS       DAJSNV010000019        34149 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSNV010000019 DAJSNV010000000
VERSION     DAJSNV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.020"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..1052
                     /locus_tag="ON516_000930"
     CDS             87..1052
                     /locus_tag="ON516_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3344427.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1109..1339)
                     /locus_tag="ON516_000931"
     CDS             complement(1109..1339)
                     /locus_tag="ON516_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3344428.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1425..2312)
                     /locus_tag="ON516_000932"
     CDS             complement(1425..2312)
                     /locus_tag="ON516_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3344429.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2401..3624)
                     /locus_tag="ON516_000933"
     CDS             complement(2401..3624)
                     /locus_tag="ON516_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3344430.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3641..4687)
                     /locus_tag="ON516_000934"
     CDS             complement(3641..4687)
                     /locus_tag="ON516_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3344431.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4851..6578)
                     /gene="ptsP"
                     /locus_tag="ON516_000935"
     CDS             complement(4851..6578)
                     /gene="ptsP"
                     /locus_tag="ON516_000935"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3344432.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6578..6844)
                     /locus_tag="ON516_000936"
     CDS             complement(6578..6844)
                     /locus_tag="ON516_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3344433.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7323..9557
                     /locus_tag="ON516_000937"
     CDS             7323..9557
                     /locus_tag="ON516_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3344434.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9705..10025
                     /locus_tag="ON516_000938"
     CDS             9705..10025
                     /locus_tag="ON516_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3344435.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10230..10453)
                     /locus_tag="ON516_000939"
                     /pseudo
     CDS             complement(10230..10453)
                     /locus_tag="ON516_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(10651..12231)
                     /locus_tag="ON516_000940"
     CDS             complement(10651..12231)
                     /locus_tag="ON516_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3344436.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12632..14008)
                     /locus_tag="ON516_000941"
     CDS             complement(12632..14008)
                     /locus_tag="ON516_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3344437.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14136..15254)
                     /locus_tag="ON516_000942"
     CDS             complement(14136..15254)
                     /locus_tag="ON516_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3344438.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15396..15830
                     /locus_tag="ON516_000943"
     CDS             15396..15830
                     /locus_tag="ON516_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344439.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15908..16441)
                     /locus_tag="ON516_000944"
     CDS             complement(15908..16441)
                     /locus_tag="ON516_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3344440.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16535..17713)
                     /gene="mutY"
                     /locus_tag="ON516_000945"
     CDS             complement(16535..17713)
                     /gene="mutY"
                     /locus_tag="ON516_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3344441.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17706..18503)
                     /gene="recX"
                     /locus_tag="ON516_000946"
     CDS             complement(17706..18503)
                     /gene="recX"
                     /locus_tag="ON516_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3344442.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18610..19992
                     /gene="rlmD"
                     /locus_tag="ON516_000947"
     CDS             18610..19992
                     /gene="rlmD"
                     /locus_tag="ON516_000947"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3344443.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20166..20744)
                     /locus_tag="ON516_000948"
     CDS             complement(20166..20744)
                     /locus_tag="ON516_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3344444.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21066..21536
                     /locus_tag="ON516_000949"
     CDS             21066..21536
                     /locus_tag="ON516_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3344445.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21644..22870)
                     /locus_tag="ON516_000950"
     CDS             complement(21644..22870)
                     /locus_tag="ON516_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3344446.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23624..23824)
                     /locus_tag="ON516_000951"
     CDS             complement(23624..23824)
                     /locus_tag="ON516_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3344447.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23958..24566)
                     /locus_tag="ON516_000952"
     CDS             complement(23958..24566)
                     /locus_tag="ON516_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3344448.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24651..25121)
                     /locus_tag="ON516_000953"
     CDS             complement(24651..25121)
                     /locus_tag="ON516_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3344449.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25169..26083)
                     /locus_tag="ON516_000954"
     CDS             complement(25169..26083)
                     /locus_tag="ON516_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3344450.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26229..27032
                     /locus_tag="ON516_000955"
     CDS             26229..27032
                     /locus_tag="ON516_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3344451.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27210..28157
                     /locus_tag="ON516_000956"
     CDS             27210..28157
                     /locus_tag="ON516_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3344452.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28225..28518)
                     /locus_tag="ON516_000957"
     CDS             complement(28225..28518)
                     /locus_tag="ON516_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3344453.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28546..29211)
                     /locus_tag="ON516_000958"
     CDS             complement(28546..29211)
                     /locus_tag="ON516_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3344454.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29351..29983)
                     /locus_tag="ON516_000959"
     CDS             complement(29351..29983)
                     /locus_tag="ON516_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3344455.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29990..31057)
                     /locus_tag="ON516_000960"
     CDS             complement(29990..31057)
                     /locus_tag="ON516_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3344456.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31054..31785)
                     /locus_tag="ON516_000961"
     CDS             complement(31054..31785)
                     /locus_tag="ON516_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3344457.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31954..32433)
                     /gene="greA"
                     /locus_tag="ON516_000962"
     CDS             complement(31954..32433)
                     /gene="greA"
                     /locus_tag="ON516_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3344458.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32569..33744)
                     /gene="mltG"
                     /locus_tag="ON516_000963"
     CDS             complement(32569..33744)
                     /gene="mltG"
                     /locus_tag="ON516_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3344459.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            33955..>34149
                     /locus_tag="ON516_000964"
     CDS             33955..>34149
                     /locus_tag="ON516_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3344460.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT        10234 a         6912 c         5656 g        11347 t
ORIGIN      
        1 gaacagaact atagaattcc ctaaaacttg tccattattt caaaatttgt tatagtcttt
       61 ttattcaaag cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc
      121 tgccgggtgt ctatcttatg aaagaccgag atggtaaggt catctatgtt ggtaaagcaa
      181 aacggctcaa aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag
      241 tattacgtat gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag
      301 atgctttatt gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa
      361 tgaattattt cagtaattat aattatgtac atattaccaa taagggattt gtattaacag
      421 atactcctac ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt
      481 tgcgtataat ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt
      541 tgcgagtcca gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc
      601 aagggctgtt tcaaggccga aataaaaagc tactgactta cttgaaaaaa agacaacaac
      661 atttcatcta tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag
      721 ttacttattt tattagacgg atccaggaac aaaagcagtt tttaagaaca ccatcgctca
      781 ccttcagcat gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg
      841 gtcaacttgc agaaacaatg attacctctg gtgacgtttc tcctgacttc tattatgaaa
      901 agaaagaagc tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac
      961 aaatcttgat cagttaccgc aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa
     1021 acaaaaaaga agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt
     1081 caacttcaaa tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta
     1141 cgatatcgat attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg
     1201 tatcggtcgc tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc
     1261 gaacttgcag ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga
     1321 ctaattgtga atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa
     1381 agcatagcct acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct
     1441 ggtatttttc cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa
     1501 cctttatctg ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaaga
     1561 tccatttttt ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa
     1621 aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta
     1681 gctgcacttc cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt
     1741 gcatagacaa gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc
     1801 atttttgtat gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct
     1861 ttgaataaag gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt
     1921 gtaccattct ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct
     1981 ttttcttttg ctgtttttgc aattttttct gccaggcttg gtgtatttgt agtcaaatca
     2041 actaaaatat gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct
     2101 tctacatctt tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct
     2161 tttggtgtat ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc
     2221 cgattataga cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc
     2281 atgacgcctg ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc
     2341 cttgcttcta tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt
     2401 ttatctttta aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt
     2461 tcttcgcatc tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc
     2521 gccgaaatgt gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg
     2581 aacttcactg gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt
     2641 tttccctaat gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca
     2701 aggtgttccg gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc
     2761 actgacaaaa taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc
     2821 tgtgaactgc acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac
     2881 ataaggtcct tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg
     2941 aaacccatta atcaaggctt ggatattttt ctcataagaa atcctgctta aagaaagaat
     3001 catcaaatgg ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt
     3061 gatatcagaa cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc
     3121 tattacacca tagctgcgta atgtatcaat aacacgttca cttggacaaa ctacacctgt
     3181 cgtatgattt gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc
     3241 tttaccgata taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat
     3301 tttcaacttc ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg
     3361 aataagatct agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac
     3421 aactcttcga tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc
     3481 caacttatcc gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc
     3541 taattcttgt tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt
     3601 atcggtaaaa aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta
     3661 ctttttttaa tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt
     3721 cttcaccgat tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac
     3781 tcggtagttc ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc
     3841 ccatatagca gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa
     3901 gtacgttttg tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag
     3961 aagggaaaaa gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt
     4021 aaccaggaaa caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag
     4081 gaatcgagta catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag
     4141 caatttctac gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta
     4201 ctttttgatc cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag
     4261 ggtgatattt ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggataccat
     4321 agctttccaa caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt
     4381 acaatcctac cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat
     4441 ttcggaaatc ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg
     4501 ctttattgac catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat
     4561 cttctttttc atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat
     4621 caagcgcacg cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata
     4681 ccttcacctt acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca
     4741 aacttaagcc ctatttttgt ctattcttac tgacaaaaac gttttcttaa aaaagaacaa
     4801 aaaaactggc gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca
     4861 tattcatgaa caagttcaac gacttcttcc atcatatcgc attctttcaa tgcacgatca
     4921 gcaagttcag ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata
     4981 gatgtcgcac tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt
     5041 tgatctccag ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca
     5101 attacatttt tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg
     5161 cgttcgttca tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag
     5221 aagaaatcta cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc
     5281 atgatgccga cttggatcgt atcagatact tccttgcctt cacttatcaa tttttgtttt
     5341 tcttcttcaa agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac
     5401 atgatacgta aattaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac
     5461 atttcgtcgc cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc
     5521 atttcatgag gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga
     5581 acaactacag gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca
     5641 tcttctgttg ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact
     5701 gcttctccac cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct
     5761 aattcaaaat gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct
     5821 gctttttgtg ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta
     5881 ggatcgatga ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact
     5941 ttagcagtaa tttcttttgt cccaacgatt gctggaattt ctaaagaacg tgccatgata
     6001 gcagaatgtg aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct
     6061 aattgtgctg tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc
     6121 attgatggat ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacgtca
     6181 cgaatatctg cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg
     6241 atatacatat ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata
     6301 ttttgcttga tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat
     6361 acttgcgctt ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct
     6421 tcagtagatt tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact
     6481 gttgccttac tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca
     6541 ccgtcactag cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct
     6601 tcattgtttc aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag
     6661 taacatcaga accttgacct acacctaaag acataacgcc catgattgat ttcaagttta
     6721 ctgatttacc tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta
     6781 atagtgtagc tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt
     6841 ccatattaat cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag
     6901 taattcagag aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc
     6961 gttagataca acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct
     7021 ttttcatcct tttcttcaca ttttataaca aacgtgttta tttccttttt cttgtcgctt
     7081 tctcttctcc tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga
     7141 attacttgct tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat
     7201 agagatttca ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc
     7261 tcatgaaaaa cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat
     7321 tcatgctttg tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca
     7381 atggtcagcg gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac
     7441 aaaatggagg tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg
     7501 atcttttccg ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac
     7561 caacacaatt tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag
     7621 aaggtttatt gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg
     7681 atcctgtgat cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa
     7741 ccaagaacaa tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag
     7801 gtttagcaca aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca
     7861 ttcgtttaga tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac
     7921 gtatgcaaaa attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg
     7981 aagttcacga aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata
     8041 ttttaaaacc agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg
     8101 agtaccgcat catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag
     8161 atgaaccaac tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag
     8221 actatcatca tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc
     8281 gttacgtcca agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt
     8341 ctaagaaaaa cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag
     8401 aagcagaaga acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt
     8461 atcgcgatca gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa
     8521 cacctgtgat taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag
     8581 tgggtgaatt aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag
     8641 ctcatgtcat cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc
     8701 gtgttggatt aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg
     8761 gtgtaggtaa aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt
     8821 caatgattcg tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg
     8881 gttctcctcc aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga
     8941 gaaatcctta cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc
     9001 atatgttctt gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca
     9061 gctttaaaga tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa
     9121 acgttggttt tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca
     9181 actactttac accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat
     9241 caaaagaaaa cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg
     9301 cagcacagca acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag
     9361 gctatgatcc atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag
     9421 atggtatcgc tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg
     9481 ataaagatgg aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg
     9541 aagaaacggc tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc
     9601 gtctcattct tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa
     9661 taagagaaac aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg
     9721 taacaaacag ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag
     9781 tcaaggtcta cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg
     9841 aaattctgct gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa
     9901 attatttctt agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg
     9961 aattggcagg ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg
    10021 tgtaaacaaa ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt
    10081 gtctcacgct ctaaacacgt ataaacggcg aaagcagaag caactccttc ggaaataagc
    10141 tgaaattcac gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta
    10201 aacgcttctg tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt
    10261 acttttctct tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta
    10321 tggatgattg atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct
    10381 aataatcgac ataatcgtaa catctcttct ttttctgttt ttttctttca cgtctttgcc
    10441 attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa aataattttt
    10501 atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc gtcacggaaa
    10561 atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat tcttattact
    10621 tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt ttacattggg
    10681 atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca acggttggtc
    10741 gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca aatcttctgg
    10801 atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg cgttatactc
    10861 atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc caatgatata
    10921 ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta attgatagat
    10981 tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca taaacagctc
    11041 tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga acaatgtatc
    11101 cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta ctgctttttc
    11161 gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca gttttttatt
    11221 tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga ttcggacaaa
    11281 agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata cgaaacctga
    11341 aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc cataaggagc
    11401 tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag tcaatgcaga
    11461 accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa atgcatctcc
    11521 agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat tttcagttag
    11581 aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt catcctgata
    11641 attttccgga cgataaaact ctacacgatt atggtagata tcataaagac cttctagccc
    11701 tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata gctcttctaa
    11761 ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga taaaagtaaa
    11821 tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg cttcgatccc
    11881 ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac gataagtatc
    11941 ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc cttgataatc
    12001 aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt ccatccagtc
    12061 tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac ggatagcccc
    12121 accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat gggaaatgat
    12181 cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca taacctaact
    12241 ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg atctcttaaa
    12301 gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt tttccgagta
    12361 aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa atacgaaaag
    12421 ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac aacctttgtc
    12481 aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc accaaaccat
    12541 aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct tgtttattca
    12601 agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt
    12661 cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa
    12721 aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat
    12781 tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat gctgtaatca
    12841 aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga
    12901 actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa
    12961 gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt
    13021 ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct
    13081 gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga acgaataaag
    13141 gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta
    13201 gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga
    13261 ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat
    13321 caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag
    13381 aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca
    13441 tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga
    13501 tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac
    13561 caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa
    13621 tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa
    13681 tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg
    13741 ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga
    13801 ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat
    13861 ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg
    13921 aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca
    13981 gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt
    14041 agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat
    14101 tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc
    14161 ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg
    14221 cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct
    14281 actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta
    14341 ggataaatga tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat
    14401 gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt
    14461 cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc
    14521 acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct
    14581 tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg
    14641 tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc
    14701 aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt
    14761 ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat gattgtcccg
    14821 taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat
    14881 tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc
    14941 aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct
    15001 gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc
    15061 aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca
    15121 aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc
    15181 atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt agactgtcta
    15241 agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa
    15301 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
    15361 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
    15421 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
    15481 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
    15541 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
    15601 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
    15661 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
    15721 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
    15781 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
    15841 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
    15901 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
    15961 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
    16021 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
    16081 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
    16141 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
    16201 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
    16261 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
    16321 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
    16381 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
    16441 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
    16501 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
    16561 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
    16621 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
    16681 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
    16741 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
    16801 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
    16861 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
    16921 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
    16981 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
    17041 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
    17101 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
    17161 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
    17221 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
    17281 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
    17341 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
    17401 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
    17461 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
    17521 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
    17581 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
    17641 cataccattg aataaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
    17701 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
    17761 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
    17821 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
    17881 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
    17941 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
    18001 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
    18061 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
    18121 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
    18181 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
    18241 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
    18301 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
    18361 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
    18421 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
    18481 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
    18541 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
    18601 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
    18661 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
    18721 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
    18781 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
    18841 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
    18901 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
    18961 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
    19021 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
    19081 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
    19141 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
    19201 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
    19261 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
    19321 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
    19381 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
    19441 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
    19501 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
    19561 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
    19621 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
    19681 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
    19741 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
    19801 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
    19861 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
    19921 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
    19981 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
    20041 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
    20101 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
    20161 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
    20221 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
    20281 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
    20341 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
    20401 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
    20461 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
    20521 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
    20581 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
    20641 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
    20701 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
    20761 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
    20821 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
    20881 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
    20941 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
    21001 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
    21061 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
    21121 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
    21181 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
    21241 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
    21301 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
    21361 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
    21421 tgcttatgta tcagttgtgg ctgcatcatc gcctgacaat gtggctcatg attccttatt
    21481 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
    21541 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
    21601 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
    21661 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
    21721 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
    21781 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
    21841 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
    21901 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
    21961 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
    22021 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
    22081 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
    22141 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
    22201 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
    22261 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
    22321 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
    22381 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
    22441 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
    22501 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
    22561 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
    22621 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
    22681 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
    22741 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
    22801 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
    22861 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
    22921 aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt gtgtaacgat
    22981 tattattttt taaaatttct caaaattctt aactatcata ccataccgtg aaaaagatcg
    23041 aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt ttttccagaa
    23101 aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc acataccttt
    23161 cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc acgacaattt
    23221 cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt ccagctcact
    23281 gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt taatggatag
    23341 atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg acatggtaat
    23401 gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa aactgaaatt
    23461 tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag ttaggacatg
    23521 tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa atagacaaaa
    23581 agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat ttagtcactt
    23641 cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct tgaccttctt
    23701 ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg tcatctccgc
    23761 cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc gttccgtgtt
    23821 ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa cattataccc
    23881 aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt ttaagactga
    23941 tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc atcccaatta
    24001 ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt caaataataa
    24061 gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa tggagaatct
    24121 tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca agcccatcca
    24181 gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga aaaatcacca
    24241 aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc atttggtgcc
    24301 atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg tacagctgtc
    24361 ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt tttttcgcct
    24421 aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt gtgatgctta
    24481 tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc atatgcataa
    24541 ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt cttaataatg
    24601 tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc ctagtcgtct
    24661 ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat aaacaacttg
    24721 ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc cacatactca
    24781 ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc gtaagaaagg
    24841 atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt cggtgatttt
    24901 gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca aaaccacgaa
    24961 atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc aaaactaaca
    25021 aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc ccaatatcct
    25081 tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc taccttatta
    25141 aaaattctta tttatcatac tctatatttc aataactttc atctttattt aaaaaaatta
    25201 attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta ataccatatt
    25261 ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt agtagctgat
    25321 cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc caaatatgga
    25381 catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt ttttcatagt
    25441 cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc attatcgctt
    25501 ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag tagatatcga
    25561 aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta tcacttaaca
    25621 aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt aataataata
    25681 ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa tgactattta
    25741 tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag gataatatga
    25801 tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag ccatacgtac
    25861 gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa ataattgcca
    25921 atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct gacataatcc
    25981 ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca aatagttttt
    26041 ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt atattggata
    26101 gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct gtttccttaa
    26161 agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact actagatgag
    26221 gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc aaagacctaa
    26281 aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt ttgagtataa
    26341 ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt caattggatg
    26401 ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag cttcaaaccg
    26461 aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt gatccagagg
    26521 gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt agtgtcggga
    26581 tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct gctttgttga
    26641 acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta acaggtaagc
    26701 agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc ctgacattta
    26761 tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt accaacattg
    26821 ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat tgtttaaaaa
    26881 cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg gtctttcttc
    26941 cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt gctcaagctg
    27001 tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt tatttctact
    27061 tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt ttatttgaaa
    27121 aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta tgcgctacac
    27181 ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat gcagatcgtc
    27241 catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat atctttaaaa
    27301 cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg aactcagact
    27361 accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc tcagctggtg
    27421 ttgatcagct accccttgag actttttcaa agaaacatat tttactttca aatggtagtg
    27481 gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg tattctcgtg
    27541 gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca tcggtccatt
    27601 accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt ggaaaaatga
    27661 ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca tccggtcatc
    27721 cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt cttcctgaag
    27781 tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc tttgatcagc
    27841 atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt ggtgcttctg
    27901 ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt gcagcattag
    27961 acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg gacaatgtac
    28021 tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta atggctattt
    28081 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
    28141 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga gaaggtgaca
    28201 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
    28261 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
    28321 gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg gctgttcaac
    28381 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
    28441 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
    28501 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
    28561 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
    28621 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
    28681 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
    28741 aggattggag actcggattt ctgctaaaga atatttatct gacaactcta ggtaatctaa
    28801 aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct cgggatggac
    28861 gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt gggcttttgc
    28921 taaaatattt ggtacgccca tttcctttac catcagagta actaagatac tggcttgaat
    28981 atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg atcgaagtgt
    29041 catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
    29101 ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca gtgttcggca
    29161 gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca ccgttttcct
    29221 cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa gcaagcatca
    29281 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
    29341 atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct tgtgtacggt
    29401 cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt ttcagtgtaa
    29461 taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc attaaaattt
    29521 cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga ctcatttttt
    29581 ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc gttgccctta
    29641 tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct gcacctgctt
    29701 caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg attttagctt
    29761 ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct tccatgacta
    29821 agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct tgtcgtccat
    29881 tttcagcttc tccaattact tcaatgtctt cttgaataga taaataagaa gagacgccta
    29941 agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc tatgcctcct
    30001 tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta atgattttta
    30061 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
    30121 tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa agattattgt
    30181 ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat gtatttgata
    30241 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt tttacatctt
    30301 ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat aactgctcga
    30361 taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga agcagcgcac
    30421 gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc ttgcgaaaca
    30481 tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc atcattgctg
    30541 caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt ctttctaatt
    30601 ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt acaatttgta
    30661 attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta tcaatatctt
    30721 tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg gattcataat
    30781 tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc ttttgaataa
    30841 gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac aaatggaaga
    30901 taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga ctttgattgc
    30961 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
    31021 aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct cgacatctcc
    31081 aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat cactgctgta
    31141 gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga catttcctac
    31201 taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc gtgttctgcc
    31261 tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat tgcccaaatc
    31321 tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat agacgtcgtt
    31381 tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat gtgtttttaa
    31441 ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt tggttaaatc
    31501 tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata cagccatcat
    31561 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta atatcaattg
    31621 aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat tgaaaatacc
    31681 gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
    31741 cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc tttctccctc
    31801 ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag gactttagat
    31861 gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga gtgtatcatc
    31921 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
    31981 aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac gtttgccaat
    32041 caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat ctgcttcagc
    32101 gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga atgtaactgt
    32161 tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt gagcaaaacg
    32221 aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttcgctga
    32281 ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat
    32341 gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt tttctttacc
    32401 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
    32461 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttcttc
    32521 aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct aagaattatt
    32581 tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat aaacatttcc
    32641 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
    32701 gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat tggtatacaa
    32761 attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt gttcacctaa
    32821 tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc ggttaaagaa
    32881 gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
    32941 tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat aatagttttg
    33001 catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg aagtactcgt
    33061 gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg tgttctcagc
    33121 tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa agaaagtctc
    33181 atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg ctacccgttc
    33241 agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg caacttttgt
    33301 tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag tcatattagg
    33361 cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg tataataatt
    33421 gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa tctgcttgtt
    33481 agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat ctaaaggctt
    33541 caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa taatcaataa
    33601 tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt tacgcagatt
    33661 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tatctttact
    33721 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc gtaactttat
    33781 tcattataca tgatatttga attggcgtga atgattttat ttaaatattc tatcaaaaat
    33841 gtaagtagta aatgataaag tccgtataat tgtgtaaaag taaaaaggcc atataacagt
    33901 ccttttacgg tacaatgttt ttaacgacaa aaacataccc aggaggactt ttacatgacc
    33961 caagtacatt ttacactgaa aagcgaagag attcaaagca ttattgaata ttctgtaaag
    34021 gatgacgttt ctaaaaatat tttaacaacg gtatttaatc aactaatgga aaatcaacga
    34081 acagaatata ttcaagcaaa agaatatgaa cgaacagaaa accgacaaag tcaacgaaat
    34141 ggctattat
//
LOCUS       DAJSNV010000020        31242 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSNV010000020 DAJSNV010000000
VERSION     DAJSNV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.021"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(34..747)
                     /locus_tag="ON516_000965"
     CDS             complement(34..747)
                     /locus_tag="ON516_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3344461.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            922..2346
                     /locus_tag="ON516_000966"
     CDS             922..2346
                     /locus_tag="ON516_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3344462.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2645..3352
                     /locus_tag="ON516_000967"
     CDS             2645..3352
                     /locus_tag="ON516_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3344463.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3339..3662
                     /locus_tag="ON516_000968"
     CDS             3339..3662
                     /locus_tag="ON516_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3344464.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3751..4788)
                     /locus_tag="ON516_000969"
     CDS             complement(3751..4788)
                     /locus_tag="ON516_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3344465.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5109..6092)
                     /locus_tag="ON516_000970"
     CDS             complement(5109..6092)
                     /locus_tag="ON516_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3344466.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6507..7277)
                     /locus_tag="ON516_000971"
     CDS             complement(6507..7277)
                     /locus_tag="ON516_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344467.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7270..8205)
                     /locus_tag="ON516_000972"
     CDS             complement(7270..8205)
                     /locus_tag="ON516_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344468.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8521..10170)
                     /locus_tag="ON516_000973"
     CDS             complement(8521..10170)
                     /locus_tag="ON516_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3344469.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10287..12890)
                     /locus_tag="ON516_000974"
     CDS             complement(10287..12890)
                     /locus_tag="ON516_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3344470.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12902..13951)
                     /locus_tag="ON516_000975"
     CDS             complement(12902..13951)
                     /locus_tag="ON516_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR3344471.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13977..14291)
                     /locus_tag="ON516_000976"
     CDS             complement(13977..14291)
                     /locus_tag="ON516_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR3344472.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14307..14753)
                     /locus_tag="ON516_000977"
     CDS             complement(14307..14753)
                     /locus_tag="ON516_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3344473.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14754..16676)
                     /locus_tag="ON516_000978"
     CDS             complement(14754..16676)
                     /locus_tag="ON516_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3344474.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16888..17784)
                     /locus_tag="ON516_000979"
     CDS             complement(16888..17784)
                     /locus_tag="ON516_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3344475.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17777..18457)
                     /locus_tag="ON516_000980"
     CDS             complement(17777..18457)
                     /locus_tag="ON516_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3344476.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18458..19240)
                     /locus_tag="ON516_000981"
     CDS             complement(18458..19240)
                     /locus_tag="ON516_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344477.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19233..20150)
                     /locus_tag="ON516_000982"
     CDS             complement(19233..20150)
                     /locus_tag="ON516_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3344478.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20314..20637)
                     /locus_tag="ON516_000983"
     CDS             complement(20314..20637)
                     /locus_tag="ON516_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344479.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            21425..24652
                     /gene="fss3"
                     /locus_tag="ON516_000984"
     CDS             21425..24652
                     /gene="fss3"
                     /locus_tag="ON516_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3344480.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            24765..25079
                     /locus_tag="ON516_000985"
     CDS             24765..25079
                     /locus_tag="ON516_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3344481.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            25092..25466
                     /locus_tag="ON516_000986"
     CDS             25092..25466
                     /locus_tag="ON516_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3344482.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            25476..25811
                     /locus_tag="ON516_000987"
     CDS             25476..25811
                     /locus_tag="ON516_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3344483.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            25894..27234
                     /locus_tag="ON516_000988"
     CDS             25894..27234
                     /locus_tag="ON516_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3344484.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            27312..27986
                     /locus_tag="ON516_000989"
     CDS             27312..27986
                     /locus_tag="ON516_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3344485.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            27979..28143
                     /locus_tag="ON516_000990"
     CDS             27979..28143
                     /locus_tag="ON516_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344486.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            28219..29361
                     /gene="dcm"
                     /locus_tag="ON516_000991"
                     /pseudo
     CDS             28219..29361
                     /gene="dcm"
                     /locus_tag="ON516_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            29351..29641
                     /locus_tag="ON516_000992"
     CDS             29351..29641
                     /locus_tag="ON516_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344487.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            29900..31084
                     /locus_tag="ON516_000993"
     CDS             29900..31084
                     /locus_tag="ON516_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3344488.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            31081..31218
                     /locus_tag="ON516_000994"
     CDS             31081..31218
                     /locus_tag="ON516_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3344489.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         9607 a         6054 c         5785 g         9796 t
ORIGIN      
        1 aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa
       61 aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag
      121 agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc
      181 gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc
      241 cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg
      301 tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg
      361 gcttcgtaca atattatata catagtcttt tacactgata cttaatttat cagctacttt
      421 tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac
      481 cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac
      541 gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac
      601 gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg
      661 aagtttctca ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat
      721 ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc
      781 tatactttaa cataaataaa ccgataaaat ccacatcaaa attatacaca caataaaaaa
      841 gatatatata tttaaacaaa actatttact ttaagttgta agcggtgtta tcattaagtg
      901 gaaacaggag gtaaaaaaat tatgacaact atgaaaaagc taacttttcc agatgatttt
      961 tggtggggtt ccgcttggtc agctgagcaa gcagaaggaa aaggagaaac aggaaaagca
     1021 gagactatct gggatcgctg gtttaaagaa caaccttacc ggttttatca acgaatcgat
     1081 tcctctatta caaccgatca catccatcaa tataaagaag atatagaact aatgaaaaaa
     1141 actgggcata actcctttcg cgtttctatt tcttgggcac gaatgtttcc aaatggcggt
     1201 accggaaaag taaatcccaa agccatttct ttctatcgaa aattattttc cgaaatgaat
     1261 aataacggaa tcaaagtatt tgccaactta tatcattttg acatgcctgc tactcttcaa
     1321 gataaaggag gatgggaatc acgtgaagta gttgatgctt atgttcatta cgctgaaact
     1381 tgttttaaag aatttggtga cttagtttgt cattggttta ccttcaatga accacttggg
     1441 cctattttag gtacttatct tgaagatttt cattatccaa acctcattga ctttaaacgc
     1501 ggagcgcaag cagcattctt cactatactt gcacacgcaa aagcaataca agtatttaaa
     1561 gaactagctc ttcctagtaa aataggcgtt attctaaatt tgagtcctac ttttcctcgt
     1621 agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg atttatttta tacgcggagc
     1681 tttttagatc ctatggttaa agggaatttt tctaaaaagc ttatcctgtt attacaagaa
     1741 tataatcagc ttcctgaaga ttatacgaaa gaagatcttg tatgtatcta cgaaaatacc
     1801 gctcaaattc ttggactaaa ctattacgag ccacgtcgcg tcaaatctcg ttcttctatg
     1861 gtggatacat ctagtccatt tttaccagag tggtttttcg aaccacatat catgcctgga
     1921 cgacgaatga acgaataccg gggatgggaa atttacgaaa aaggaattta tgatttgtgc
     1981 atggatatca agaaccatta tggaaacatc gaagcattta tctcggagaa cggaatgggc
     2041 gttgcggatg aagaacgttt catggatgat acagggcaaa taattgatga ttatcggatt
     2101 gagtatatca aagatcatct tgcttactta tggaaggcaa taaatgaagg atgtaacatt
     2161 aaggggtatc atctttggac atttattgat tgctggtcat ggatcaacgc ctataaaaac
     2221 cgatatgggc tggtttcact cgaccttact actcaaaaaa gaacaattaa aaaaagtggc
     2281 gaattttaca agcaattaag tgatacaaat ggatttagat acgatgtaga cttgttagtt
     2341 gaataatatt ctaaaattcc acttgaagtg aacacattag aaaacaagaa taatgagaaa
     2401 taccaataac aaatactttt tcttcatact tcatttatac ttgtcagtat aagagactgt
     2461 gacaaatgta gtgatcctca gaagttaggt ctggaaatct aacttgagaa gattttttta
     2521 aactacgtcc agtctttttt atttgtttaa aattgaagtt gacaagtctt cattaaaaat
     2581 ttagaatatt cacaagtttg acgacaatta tgaaaattta tgtcgtgcta aaggagtttt
     2641 gttattggat gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt
     2701 tggttatatc ggtattggac ttgcatttgg tatcgttggg aaagctgccg ggtttcatcc
     2761 gctggtcgtc acgttgatgt ccctgctgat ctatgctggt tctgcccaat ttatcacggt
     2821 cagcatgctt gctagccata gtccactgct ctccatcgtt ctctcgacct ttctagtcaa
     2881 ttcccgaatg attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct
     2941 tcaaaatctg ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa
     3001 attgaactat actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc
     3061 ttatttgacg tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga
     3121 tccgaaaaag tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct
     3181 tcaagtcatt tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac
     3241 tttgattctg gtatatgtcg gactgatttt tatcccaagt aacttagtga ttttagttgt
     3301 aacactgatt gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat
     3361 tgctgactat tttaggttgt actttagcaa cttggctttc taggatccta ccatttgtct
     3421 tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg
     3481 tcattatgtc tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc
     3541 agatcaatct cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa
     3601 aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct ttcgttgatt cgcttattat
     3661 aaataaaaaa acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt
     3721 caaataagaa aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac
     3781 ttttcctaat atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt
     3841 catcccatct gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc
     3901 agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc
     3961 gaacatatac tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg
     4021 ctctttgatc cctacgatcg ttcccacatg tccaaatggt cggacaaggt gtttgatttg
     4081 atcaaagtag cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa
     4141 ttcacgaaaa cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt
     4201 tttcagccat tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg
     4261 cgctagttga ttcaaaatcg aaccgacgcc cccagctccg ttgatcacta agattttttt
     4321 cccgcggttg gcattttctt ctggagtcag accaaatttt tcaaataaca attcataagc
     4381 ggtcaatgag gtcaacggca aggctgcggc ttcttcatct gaaagatttt ttggtgcaag
     4441 agcgacaata cgttcgtcaa ccagttggta ttcttgattg cttcctgctc ttgtcgttgt
     4501 acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc acttcatcac ctacagcaac
     4561 aactttcccg actccatcaa accctaagat acgtaaagca tttcttatcc cgttatcttg
     4621 gcgaagtttc gtatcgacag gattaacaga tactgcgttg acttttacca gaagatcccg
     4681 tgaactgggt acaggtatct gttctttttc atcaataaag ctttttggat catcgatcga
     4741 caacccttca taaaaaccga ctaccttcat taattccttt tctgacatac gaattcctcc
     4801 ttagcttcat caagttggag catccataca gttttcattt caatatatat agtatgctat
     4861 ttgttccact tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
     4921 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttttaaa cggtgttcaa
     4981 tcaactgacc ctcggtattc gctcaaaaaa cgacaaaata tgaggagcta tttttgtttg
     5041 gctgttctta ttacttgatg caggacagct ttttcacgac ctctaattca cggtgttcaa
     5101 gcaagaaatc attgttcact gaaaagcgtt ggatgttctt tttcaaaggt gtctaagatc
     5161 tctcgtactt catcagtaga tttcgtattc atcaactgaa ctcgcaagtc atttgctcct
     5221 ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc ctgtgatgga acgcgggatg
     5281 atttctgaat aatgatcttg cagatctagt tgcagacgaa gtagtccgat cagttctttt
     5341 tctgtatgtt ccctcggttc tttttcaaat gcataaggat tcttgaaaat cccccggcca
     5401 atcatgatgc catctacgcc atatttttcg gccaattctt ctcctgtctg gcgatctggg
     5461 atatctccgt tgatcgtgat cagtgtttgc ggagcgattt ggtcacgtaa agcaatgatt
     5521 ttagggatga cttcccaatg tgcatctact ttactcatct ctttacgcgt acgcaaatgg
     5581 acagaaagat tggctatatc ttgatgaagt aggtgactga tccattcttc catctcagac
     5641 atttccgtat agccgatacg cgtcttgacg ctgacaggca aaccgccagc tttagccgct
     5701 tcaatcaatt gtgccgctac ttctgggcga aggatcaagc cactgccttt tccacgttct
     5761 gctacattag ggactgggca gcccatgttg atatcgattc cttggaagcc catttctgcc
     5821 atagcaatgc tcatttctct aaaaaattct ggcttgtctc cccaaatatg ggcaaccatc
     5881 ggctgttcat cttctgtaaa ggtcaatctt cctcgtacac tgtcttttcc atctggatga
     5941 cagaaactgt ctgagtttgt aaactcagta aagaagacat ccggtgctgc tgcatgcttt
     6001 acgacatggc gaaatacaac atctgttaca tcttccattg gtgctaaaac aaaaaaaggc
     6061 tttggcagct ctttccaaaa attagtagtc atttatttgt tactctccat tctatcaagc
     6121 agtgaagtcg caatctttct caaaaacatt gtcattatac taggaaacaa aaaaagaagc
     6181 aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa aagactgtga caaaaatctt
     6241 atcacaattc cttttccgga ataaatggtg ttcaaaagct tgcatctgcg gtaataagtc
     6301 caaccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag
     6361 aacagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     6421 ctcaacaaac gacaaaatat gaggaactat tttcccgttt gtttctctaa tactcaggtc
     6481 aaaacgctga tttcacaacc tcttttctaa cttctcaatc ctcttccttt attgatcaaa
     6541 taccaagtaa cggcgtacaa aacaacgatg aatagcacaa gtacgatcat cgatgttgct
     6601 actggcacat cgattgttcc aaggaatccg taacggaaac cggagatcat ataaacgatc
     6661 ggattgactt tcgaaacagc ttgccaaaat ggcggcaaca tagagatggc atagaaaacg
     6721 ccacctagat aagttaacgg ctgcaagaca aatgtaggca cgatcgatac atcgtcataa
     6781 gactgagcaa aaatcccgtt gagcagtcct gctaacgaaa acaagattgc cgtcatcaat
     6841 aatgtaatga tgaccatgaa ccatgaatac acatgaagtg gtacaaagaa caaggaaatg
     6901 atcgttacga gagagccgac gagaatactg cgacccactc cgccaatcac aaagccccaa
     6961 atgatcacat gcgtaggaac aggcgctacc aagatttctt cgatgttttt ctggaatttt
     7021 tgagagaaga aggacgaaga aacgttggca tacgaactag tgatagcaga catcataatc
     7081 aaaccaggca cgatgaattc catatatgaa aaaccaccca tgtcaccgat ccgaccaccg
     7141 atcatttttc cgaaaataac aaagtacaag gatgtcgtga tcacaggcgg aaccagtgtt
     7201 tggacccaga tacgcaaata gcggttcgtt tccttgatag ctaaactttt taatgcagta
     7261 aaataaagat taaacatttt tctcctccgt atgattattt tcttctgtga ttttcaaaaa
     7321 cagttcttct aaacgattcg acttattcct catagaaaga actttgattc cttgtcttgt
     7381 cagctgatcg aagattccgt tcaccccttg atttcgttca acttcgacag cgattgtctg
     7441 ttcatcttca aatgtacaag cgtatccttc aatcacaggt ttggtctcat atggcgctaa
     7501 atcaaagata aatgtttcaa attgcagttt ggatagaagc tctttcatac tagtattctc
     7561 gatcagttca cctgattgga tgatcccgat gttgcgacaa agcatttcag cttcttctaa
     7621 ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc aattctcgga gaaactccca
     7681 catctcgcgt ctcaattcaa tatcgactcc tgctgttggt tcatccaaga tcagcaaacg
     7741 tggttcatgc atcaatgccc tagcaatcat cagtcttcgc ttcattcccc cggaaagcat
     7801 acgtgcacgg acatttctct tttcccaaag attggactgc ttcaaatatt tttcgctgcg
     7861 tttcatcgct tctttgcgag agacaccgta gtagcctgct tgattcacga cgatttgctg
     7921 gaccgtttca aagggattga aattgaactc ttgcgggact aatccaatct gttgctttgc
     7981 tcggactaga tccgtatcca gatcataacc gaagacattc acttttccag atgttttatt
     8041 gactaaagaa gtaatgattc cgatcgttgt agacttccca gctccatttg gtcctaatag
     8101 cgcatagaaa tctccttctt tcacggaaag atcgattcct cgcaatgctt cgaccccact
     8161 tccataaacc tttttcaaat tatttatttc aagcgcattt gccatttatt tcctatctcc
     8221 ttatagacat gattctttat tgtatcatgg aatacagcag atgaaaaaac aacttggttc
     8281 cagcgaaaaa tcaggttgct gggctcagga aaatctccta atgaaagaca tcaatcggac
     8341 gaagtcagac aaccttttca gaaaaaacaa atcgaagata aaggattact attcatatgt
     8401 gcaacaaaag gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
     8461 ataaacggcg gaagcaaaag ccaagatttt tgatccaata aatactttcg atcacttcgc
     8521 ctatcccaat acatgccact gccgcttttc atcaaataac gcggcttccg tttttgcggt
     8581 ttctactaaa gtctctgggt aatccaaaac tttcagcaag gcaatcgcat ttcgggtcct
     8641 tgaaggtcct tcttttaatt tgaaatcaaa actgattcct tttccttctg ttacttgttc
     8701 ttcaaagtgg acattctcac aataattctt taaaatctct gtcaattcga tatcatgtgt
     8761 ggcgacaaat gctaaagatg gatactccgc aagccaccga acgactgaag cggaagaagc
     8821 aatccgttcg atcgtatttg tcccttttaa aatctcatct acgaagcagt agcagcgttc
     8881 tttggaagcc acttggtcca acaaacgttt gatcgatttg atttctgaaa caaaataact
     8941 gtcgccttca aataaatcat cttcgattgc catggaagtc aacacatgtc caggttgcag
     9001 ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct agaatacagc tgatggctat
     9061 acttttgaca tacgtagatt ttcctgatgc attggaaccc gtgactaatg tattcttctg
     9121 ccagttgacc ggattcacta ccgcttcttt taacaatgga tgatagatat ctttcgctgt
     9181 cacaccgccc tcagtaaact ctggtcggca agtgatgggc atataagtac ggtaattcaa
     9241 tattgcagcg gctacttcta attctcccag caattcccac agcttgatgg cttcagttga
     9301 atgcttttct aatttagtga gaacgaaatg ataggagata aatggcagca tgaacatgat
     9361 gttgacatat tcaaaaatca actctgcttc actcccactc ttcatacgaa atgaaaaacc
     9421 taatttaggg atgtctctta atggtttagt ttgctgtttc agctcttctt gaagtggtgt
     9481 cggcagtttt gctagctgat tggcggctga aatcgttgaa accaaataac tcatgctatt
     9541 cagctctgtt tctagtgttt gtttcttcac tagataatag accgtattga atactaaccc
     9601 accgattgcc aaatagattc ctagtacttg tcctaaaaga agcaaaccca aacccaccag
     9661 cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca atctttttac tttttccatc
     9721 cgctagatat tgttttgcaa aattatgatc tttttttcct aaacgagcga attgatattg
     9781 gattttttct ctcagttgag gattttcctg ataaaaagca atcagttttt ctaactgctg
     9841 atcttcacca aaatcaaagc tgcgtaaacg ttgatacaac gcttctgaac ctacactaga
     9901 ataggtactg ttgattcctt cgaatacttc gaacatatcc aggtcatacc aagtaagatc
     9961 atcgatttcg ctatcccatg aacggaactt cttctccgtg agccatgctt ccttcaagct
    10021 ttcttcttta tcaaatctcg gttgataagg aattttttcc catttgctac gaactgtttc
    10081 ctttaatttc aaacgtgaaa aaatgtctaa agcaactaat aaaataacag ctgcaataat
    10141 aattaaaacg ataaattctc tttgacccat atccctctcc gttcctttat ttgttagctt
    10201 tagtatagca ctgttgcgct atttgaaaag agggtaaaaa aacacctttc aaaataaaag
    10261 gtgttttttt cttgtatatt acatatttat ctctcattta tcaaaattga caaataatca
    10321 tagggaggaa ttgttgttaa ttcttgttta ttctccagtg cattgacaac tgtcccaagg
    10381 gaaaggatag cctcaggtac tattttttct tgttctgttg gattagctaa gcgaagaaca
    10441 aatccttgtt catgaaaata agaaggataa atcgatgata caattaagtc ttcaggtaat
    10501 tcaatcaatg aaaatttacg aggaacatca agttcatttt gtaatggcca aatcttgtta
    10561 tctaacctgt gaataaatac attcagtttc tgcttttgat aagaaataga ttgttctaac
    10621 cgtttttctg cctgttttgc cactttgaac tcattaaatc ttccttcttc tacattaaat
    10681 ccaaaagtga ctttatactc acctatctct tgagctagcg gtgtttccat catgacatgc
    10741 ccttcatttg tcgtatctcc tgaagctcgc ccaggtcgcc aagcaagatt aggttttcca
    10801 agttcccctg ttgttgaaaa taaggttatg attaattgat tttctatacg ttcatactct
    10861 tttaaaccat ctgcataaaa cgtaaaataa tgatcatttt ctgccacaga gacactctta
    10921 tcgaatattt ctaaattaac aggtttctct accatagttt ctgaccaatt cggaggaatg
    10981 acatcagggg tattttcaat aaatccgttt tgaatttgtg caattgattt ttcagattta
    11041 tctaaaacat taattgctaa gcgcatacga tgcgagtaaa cagtattatg aattttgagt
    11101 gtgccttcaa cgatttgact ttctcccaga ctgaacgata tttcataaga tatttcttcc
    11161 atttcgggtt tttcagataa acgatctttt aaatctttag gaagttgagc ttttccgtaa
    11221 acaactaatg tttcttgtaa agaatcttta tatactttag ctgtttctaa ttttagagat
    11281 agttctgtat ctccttctaa tggtgaataa tcatacgtat cgccatcatt tgctgaatcg
    11341 atcaaatgaa cgaaatcaag atattgtcta tctccaacta tcaaattcaa ttgcccatct
    11401 ttaaaagaaa gtttatattt attgttactt atctcagttt tgtctatctc gattgctgtt
    11461 tctaattttt cattattttt ctcaaattca acaacagtgt atcctagtgc tggcacattt
    11521 acatttattt ttatgtcaag ttcataataa gctggttcat caaaatattc gaaacctgta
    11581 gcaatcatcc gataagcatt tttccgttct ggaaaataaa cttctttttc aatgatatta
    11641 tagggactac ctttgaagtg tattttcttt gaatcagcta ctacatgcac gattttcgat
    11701 ccactataat tctcagtcgt tgtgttgaaa acaaggactt cattcgattt tagctttaat
    11761 gcatcagcca tccgttttac aataaggttt tcgattccgt cagctatctc attagcttct
    11821 ttgattcgat ggaaaatatc ttccgcaaca ttatctgaaa cacagccacc aatactatca
    11881 tgagcctggt tttgcaaaat ctttttccat aaccgacgaa gtaggccttt actgatatgg
    11941 ataccatttc tttctgcaat cagcatgagc ggctcaactc gttttagaat tttttgttcc
    12001 aatttaaaat tttctatttt catttttgtt cttactgagc caatagaacg atgaacacga
    12061 gagtaaaccg gctgacgaag ttcaccttta tattcaggta attctttttt gtttctgata
    12121 acttctacat aagaaggata atctgaaata atattgtgga atttactgta tttattcaac
    12181 ttctctacga cttgatcaaa atttaaaatc attgactttt gatctacacc cgaaggtact
    12241 aaaatatctt tgttttttcc ttgcatatga agcatttcaa ctgcttcatc caacttttct
    12301 tttgcaaatt caggaatatc tttaattgcg tctaaggtca tcattccagt catataacca
    12361 aatggaaaat tcatggcata aacaaattta tctcccaatc ctttccagat aaaataagga
    12421 gatggaacca tcgtttcaaa gtttaatcct cgccaacaca tgaaattatc gatttctact
    12481 tgatgcaaaa gtgtaggcaa attcgcattg aacccaaacg tgtcaggcaa ataccctacc
    12541 atcattggat ctccatattt aaggatcgta tcactgattc caatcattaa gtttcttaaa
    12601 attgattcag catccactaa taaggcatct gtttgtgcat accacggtcc cacgaatagt
    12661 tgtcctgttt tcatcagtcg ttggataacc ttaagctttt gcggattgat ctccaaatac
    12721 tcatcgacga ttgaagtttg tccatctaaa caaaagttta ctttagggtt cctttccaat
    12781 tcatctagaa cttcagtaaa gacctgatca gacaatacga tggcatcttc atttgtaaaa
    12841 taccattctc tgtcccagtg cgtgtgttga attatatgtg catttaccat atcagattct
    12901 cctactttcc ttcttctact gaatttactt gttgttcttt tttatacttg atggatttta
    12961 gtgcattgac cattaacgct gtaaccactc ccccaataac aattgatact aaatataaag
    13021 gtatattcgt aacaaaagga attgccataa aggttgaaag cattgtttct tgagtaacac
    13081 caaaaaatcc atttagacca catgctactg cagtgcccac cacgatgctt ggtattactc
    13141 gtaaaggatc tttcacggca aacgggatcg tcccttcagt aatccccaca aggcccataa
    13201 tcatagctgt ctttgcatta tctcgttccg attcatcata aagttttttg tttaaaattg
    13261 ttgctaaaaa cattccaaat ggcggaatag caattcctaa taacatggct gtactaggtc
    13321 caaagcttcc gcttgaaaaa gaagcaaccc caaaagcata tgcgatttta ttgataggtc
    13381 ctcccatatc aaacgccatc attcctccta aaatagccga catcaaagca gcatttgctc
    13441 ctgacatgcc ttctagccaa ttagtcagtc ctgttgtcat tgcttgaata ggcgtagcta
    13501 gtacatacca taatatgatt ccaacaacta atgtagtaac aaaaggaatc agcataattg
    13561 attttagact aataacttgc atcggtactt ttactttttt caatgcatta gctaaatagc
    13621 cacaagctaa tccagcaacc attcctccaa taaatcccat accaagattt tgacacatca
    13681 tcccagtgat aaaagctggt gctagaccgg gacggtcagc aattgaatat gcgatatagg
    13741 ctgaaatcat cggtacgata aactgtaatc ccgtctgacc aatcattcgc aggactcccc
    13801 agaatgtatc cgtctggtcc caaacacctg tgccaccaaa aatgactgca atagaagtta
    13861 gtaaccctgc tgctacgaca agcgggatca tataggaaac cccactttgg aaatgattta
    13921 gaatttctct tccttgtttc ttgaattcca ttagtaactc ccccacgata tattttttat
    13981 ttgttaatta atgcaattgc ttcatcaatc gtctcttcac catcttttac tacctctgag
    14041 gtccctactg ttaaaatcgg tttaccgtca aagcgacctc gttctgaaac atttgtatct
    14101 actgcaaata tgacaacatc tgcctcggaa acatccgaaa ttgaaagttt gttttctatc
    14161 cccatagccc cctgtgtttc tactttaata tctaaccctt tcttcttcgc aaacttttct
    14221 aaattagctt ttgccatata agtatgggca atccctgcga tacatgctgt tactgctact
    14281 atttttgtca tatgaaattc ctccttttaa aatgatagag cttttaccaa ttctttttta
    14341 tttctcgaat cttttattct tttaacaaac tcgtcatcca tcaatcttcc agataattct
    14401 gctaaaatac gtaaatgatt agtaccttta tcagtgtctt ttattgctaa caaaaagacg
    14461 agatcaactg gttgatcatc taatgcgccc cattcaacag accgctttaa cttggctaca
    14521 gccactgttg attcaattac tgctaggctt ttgccatgag gaatggcgat atttttccca
    14581 attcctgtgg tcatatggtt ttctctgtca aatacgtctt tttgaaattg tttgtggtca
    14641 gacacaaacc cattagtctc taacaatttc gataattttt cgactacact ttttttatca
    14701 gatgcattta aatctatcac tatattattt atattgattt tttcactgat catttataag
    14761 ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa atttgctaat
    14821 aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa ttcaggtttt
    14881 actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc ccaatcaatt
    14941 ccttctggat taacgtagac acagattact ggctttttta taaaatctac ttcgatatgc
    15001 ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat tgctccctct
    15061 agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat atttaaaacg
    15121 ttcacttgat tatcctttat ttggtgggta aaaaagcaaa atcgtttatc taataaattt
    15181 agaaaagcat cttctgtttg ttcatttaac tgtagatact gttttacttt tctgacatct
    15241 gcccctaaca tcaaaggact aacaacaata acaggaaaat ttgtattttc aatcggaatc
    15301 gtgcttataa caagttcatc agtgactttt ctatcttgta gttcattgat gcttagtaca
    15361 tcttctatct caaccatatc tgcaaaaccg ttatttattc tttgctctaa taattttgat
    15421 gttccatatc cgctactaca aactaatatc actttcgttt tgtcaggttt gtaacgatcc
    15481 aaaagtgatt gaatatggag tgttacgtaa gcgatttcat cttcattaaa atgtatacag
    15541 aatctctctt cgatttcctc taataaatct acacttatca taaatgacct tttaaaagaa
    15601 tatttgattt tgtccgtata agggttatag atatttactc ccaatttgag tcttttaact
    15661 gctgcattga gatgtaatac tagattattg atcaattctt catcaggata aaattcattt
    15721 aatttaattc ttactaactc ttgtagctca acacccatat ctaaatagga aagcttcgtt
    15781 tgatctaagt cgtgattcat agcatttttt tcaatcgaag taatgtgaat gtcaacataa
    15841 gcttgttcat ctattggcac aataattgaa aatgcttttt caatcatttt gattaaaata
    15901 agtgtattct ttgagatttc ttcttcagaa ttattactac gtgataagaa attgcttgtt
    15961 tcaatccttt ctatcgcaat agcaaggtgg atagctaaag tttgaaattc ataatctgta
    16021 aatttcaatt gtgtcttatt catgtatcga ttaagcaatt cggctacttt tttcaatatg
    16081 tccttattta agaaattatt cgcttcacca atgaaattga tattcaagtc taagagatct
    16141 gttttactgt tagatgctgt aaagccaccc caataatagt tcacaacttc tgacatcaac
    16201 cttcttttac ttttttcatc agctttgatg cgcacgccat ttcgatctga aactaaggac
    16261 atcccatgct tgtttagtct ttttctaaca tctttcattg tgctttctac cgtcgacctg
    16321 ctcacgtata attcgtcagc aagattttga atctttagag aatcattagt attcaataat
    16381 ttagcaaaca cataagttag acggtcctcc ggagagtctg cgatattgat tttatgactg
    16441 gataataaat ttcgaagtgt ttcttttttt ccaataagcg aaacgccttt tttaggttga
    16501 accaacattt gtacatctgg cattttttgg attatttctt tcaaaattaa aatatctttg
    16561 ctaattgttt tcgtagatac atataattgt tctgataatt ctttgtaagt tgtgtgctca
    16621 ttttcaatca aaaagtatag tattttcatt tgccgttcgc taaattcgat gcccattatc
    16681 ttcacctcct gaatacagct taagtgtaca attattatct ctattttcat acagaagaaa
    16741 gttcttgatt aatgtagaag tttctaatat tcttgattat ttcaaatcaa taaaaaagcc
    16801 tgcaatattt atatcacagg cttttttctc tattaaatat atataagata atacattttt
    16861 ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg agatggagaa
    16921 gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga
    16981 caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa
    17041 acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat tggccactgt
    17101 taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg
    17161 caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac
    17221 agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc
    17281 ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat
    17341 ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa
    17401 tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac
    17461 ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga
    17521 aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg
    17581 ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat tcttttctcg
    17641 aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt
    17701 agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac
    17761 gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta tgaatataca
    17821 cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc actgttttct
    17881 cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt
    17941 ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag
    18001 ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga
    18061 tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc
    18121 gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca
    18181 acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg
    18241 atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta
    18301 gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag
    18361 catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga acagcttcat
    18421 cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca gatttttgga
    18481 aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt
    18541 tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg
    18601 taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaatgaagg
    18661 ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga
    18721 atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat acaggaaata
    18781 cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa
    18841 gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt gtgatcaaaa
    18901 gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat
    18961 tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag
    19021 ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca ccgttgatga
    19081 ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc
    19141 aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct
    19201 tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat
    19261 tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac aggaactttt
    19321 gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata
    19381 acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac cgttactgct
    19441 tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct
    19501 ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt ggcgatctgt
    19561 gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa
    19621 cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca
    19681 tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc
    19741 atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc
    19801 agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta
    19861 tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt
    19921 cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc
    19981 aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc aatcaatcca
    20041 tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc agcaccgtat
    20101 tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt
    20161 ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat
    20221 aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa tacgtgtctt
    20281 tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt tcttttcgac
    20341 caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca gatcaagctt
    20401 gagtaatttt accagaccgg ttataaggat aactatagca agactccctc ccagaaaaat
    20461 tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac caaacagtgc
    20521 taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa gtcgtccttg
    20581 tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag aaaacattaa
    20641 tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca attagctacc
    20701 aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa acggtgttct
    20761 cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc aatttttgta
    20821 aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag cggataaata
    20881 atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat gatattatag
    20941 accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt ccaaattgtg
    21001 gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc gcccagtacg
    21061 attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga actgtgaagc
    21121 tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc
    21181 ttgatttata aaggttttcc cgtaaatata ttttattata tttgattaaa tttgaaatgt
    21241 ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta gattaaattt
    21301 ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta aaaagacttt
    21361 ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga aaggagaaca
    21421 tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt
    21481 tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa ttacccatcc
    21541 acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag gaacttttag
    21601 tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca acggctttaa
    21661 acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca ctcctggcta
    21721 tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg ttttatggaa
    21781 aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct ggcaagaagt
    21841 taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa atatcgcagc
    21901 cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa ccttccataa
    21961 tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa
    22021 tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg
    22081 taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa
    22141 gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat
    22201 ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg aaacggaggg
    22261 ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt
    22321 ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat
    22381 tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc
    22441 ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag gcgaaaccat
    22501 ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata aaagcggagt
    22561 tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat
    22621 tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca
    22681 tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga
    22741 aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta
    22801 tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac
    22861 tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc
    22921 agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag ctgttaaatg
    22981 gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg aaacggtcac
    23041 tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca acgagtatta
    23101 ttggcaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt atcctgtatc
    23161 catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa
    23221 agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag ctgcgggcac
    23281 taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga
    23341 aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg
    23401 ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc
    23461 agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga
    23521 agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat
    23581 taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa
    23641 ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg
    23701 gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag
    23761 ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga
    23821 tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac
    23881 aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac
    23941 cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga
    24001 agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata accaagccca
    24061 aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc acctagaaaa
    24121 cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg tgtcgattac
    24181 tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc
    24241 ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag taaatgataa
    24301 agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac
    24361 caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg gaaaacacaa
    24421 tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag
    24481 tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtcccac
    24541 agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt tcattggttt
    24601 tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga
    24661 gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc
    24721 gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg
    24781 tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa ggagaaatta
    24841 taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca
    24901 gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt
    24961 ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga
    25021 ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg
    25081 aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga
    25141 agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg
    25201 gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc
    25261 aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt
    25321 tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta
    25381 attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa ggaaatccta
    25441 cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt
    25501 tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc gttaaagcat
    25561 taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga gaaaatagcg
    25621 acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct ttctttatag
    25681 ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa gaaattggtg
    25741 tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg aaacaactcc
    25801 cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg aaatcctatt
    25861 gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca
    25921 ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt
    25981 tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa gctatcactt
    26041 tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg aagtctcgct
    26101 ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg gcgaagcttg
    26161 tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga agtgaaacaa
    26221 actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga gactatcagt
    26281 tacttcccca aaatctatta tcgaatgaaa gatggattac tgtctattcg cgttcaaatt
    26341 tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga aagcggactg
    26401 tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac tttactgtat
    26461 gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg gactctgcga
    26521 ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat cgctggtggc
    26581 acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt gaagtcagat
    26641 gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg tacggtgatg
    26701 cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt
    26761 atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg agagaattat
    26821 gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc ttatatggag
    26881 atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg
    26941 ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc gaaatattta
    27001 ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat gagtgaactc
    27061 ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt
    27121 cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca
    27181 aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc
    27241 ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg
    27301 ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata
    27361 agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta
    27421 tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg
    27481 ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa
    27541 aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg
    27601 tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa
    27661 aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag
    27721 accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc
    27781 cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga
    27841 gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat aatatttccg
    27901 aagttcatta tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag
    27961 atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac
    28021 aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt
    28081 aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa
    28141 tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct tttttctttg
    28201 caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg tggctttcgt
    28261 ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga taaatttgct
    28321 agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca tgacatcaca
    28381 agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg cggcggattt
    28441 ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac tcgaggtact
    28501 ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt attccttgag
    28561 aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat cctccgaacg
    28621 ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc ctacattccc
    28681 caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac cgaacaagta
    28741 tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt ggtaaatatc
    28801 aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt agcgccaacc
    28861 ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat ctctgtagca
    28921 ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac tggaaccgcc
    28981 cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa
    29041 tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc cacaagtcgc
    29101 cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca acaagcgatc
    29161 gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg acttcagggc
    29221 tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca gttgtataag
    29281 caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag attaaaagag
    29341 ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga ttggaaagat
    29401 tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata ttcgcaagaa
    29461 tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg ctcgaattga
    29521 aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag aaccagatat
    29581 tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta cagatgagta
    29641 acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat
    29701 cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt ctcacccccg
    29761 tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa gaatgcttgt
    29821 tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa gttaacacct
    29881 tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat taaaagaccg
    29941 acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac ttagtcggcc
    30001 gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca tgaggaaaat
    30061 ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt ttgactatgt
    30121 aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc tccatttgaa
    30181 atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac agtatgtcat
    30241 gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt tagaattaaa
    30301 ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta gctggtacga
    30361 ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact tggcaatcaa
    30421 tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga aggaagaatg
    30481 tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa aggctgatga
    30541 aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg tttatttctg
    30601 tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag accaaactga
    30661 gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg cgattcaaga
    30721 tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc gttacttgcg
    30781 atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg aacaatgggg
    30841 gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa aaccgtatac
    30901 cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga aaatggctaa
    30961 ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga atgcacgctt
    31021 atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa atttgattat
    31081 atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt tggtggcagt
    31141 atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga tcgagcaatg
    31201 gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DAJSNV010000021        30365 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSNV010000021 DAJSNV010000000
VERSION     DAJSNV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.022"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..188)
                     /locus_tag="ON516_000995"
     CDS             complement(<1..188)
                     /locus_tag="ON516_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344490.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVS"
     gene            complement(176..1528)
                     /locus_tag="ON516_000996"
                     /pseudo
     CDS             complement(176..1528)
                     /locus_tag="ON516_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1525..2457)
                     /locus_tag="ON516_000997"
     CDS             complement(1525..2457)
                     /locus_tag="ON516_000997"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3344491.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2454..3230)
                     /locus_tag="ON516_000998"
     CDS             complement(2454..3230)
                     /locus_tag="ON516_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3344492.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3284..6469)
                     /gene="carB"
                     /locus_tag="ON516_000999"
     CDS             complement(3284..6469)
                     /gene="carB"
                     /locus_tag="ON516_000999"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3344493.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6474..7550)
                     /gene="carA"
                     /locus_tag="ON516_001000"
     CDS             complement(6474..7550)
                     /gene="carA"
                     /locus_tag="ON516_001000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3344494.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7555..8835)
                     /locus_tag="ON516_001001"
     CDS             complement(7555..8835)
                     /locus_tag="ON516_001001"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3344495.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8854..9780)
                     /locus_tag="ON516_001002"
     CDS             complement(8854..9780)
                     /locus_tag="ON516_001002"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3344496.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9793..11076)
                     /locus_tag="ON516_001003"
     CDS             complement(9793..11076)
                     /locus_tag="ON516_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3344497.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11090..11629)
                     /gene="pyrR"
                     /locus_tag="ON516_001004"
     CDS             complement(11090..11629)
                     /gene="pyrR"
                     /locus_tag="ON516_001004"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3344498.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11864..12775)
                     /locus_tag="ON516_001005"
     CDS             complement(11864..12775)
                     /locus_tag="ON516_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3344499.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12772..13236)
                     /locus_tag="ON516_001006"
     CDS             complement(12772..13236)
                     /locus_tag="ON516_001006"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3344500.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13241..13939)
                     /locus_tag="ON516_001007"
     CDS             complement(13241..13939)
                     /locus_tag="ON516_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3344501.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13926..14312)
                     /locus_tag="ON516_001008"
     CDS             complement(13926..14312)
                     /locus_tag="ON516_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344502.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14459..16129)
                     /locus_tag="ON516_001009"
     CDS             complement(14459..16129)
                     /locus_tag="ON516_001009"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3344503.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16312..16545
                     /locus_tag="ON516_001010"
     CDS             16312..16545
                     /locus_tag="ON516_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3344504.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16644..17075)
                     /locus_tag="ON516_001011"
     CDS             complement(16644..17075)
                     /locus_tag="ON516_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3344505.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17154..17567
                     /locus_tag="ON516_001012"
     CDS             17154..17567
                     /locus_tag="ON516_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3344506.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17605..18753)
                     /locus_tag="ON516_001013"
     CDS             complement(17605..18753)
                     /locus_tag="ON516_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3344507.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18875..19471)
                     /locus_tag="ON516_001014"
     CDS             complement(18875..19471)
                     /locus_tag="ON516_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3344508.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19671..22313)
                     /gene="alaS"
                     /locus_tag="ON516_001015"
     CDS             complement(19671..22313)
                     /gene="alaS"
                     /locus_tag="ON516_001015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3344509.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22701..23132
                     /locus_tag="ON516_001016"
     CDS             22701..23132
                     /locus_tag="ON516_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344510.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23177..24526)
                     /locus_tag="ON516_001017"
     CDS             complement(23177..24526)
                     /locus_tag="ON516_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3344511.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24596..25759)
                     /locus_tag="ON516_001018"
     CDS             complement(24596..25759)
                     /locus_tag="ON516_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3344512.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25888..26211
                     /locus_tag="ON516_001019"
     CDS             25888..26211
                     /locus_tag="ON516_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344513.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26394..27743)
                     /locus_tag="ON516_001020"
     CDS             complement(26394..27743)
                     /locus_tag="ON516_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3344514.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27812..28765)
                     /locus_tag="ON516_001021"
     CDS             complement(27812..28765)
                     /locus_tag="ON516_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3344515.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28867..30057)
                     /locus_tag="ON516_001022"
     CDS             complement(28867..30057)
                     /locus_tag="ON516_001022"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3344516.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8528 a         6666 c         5338 g         9833 t
ORIGIN      
        1 ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca aaaactgatc acgatagaag
       61 tttcttttca tacctgatga aaagatgctt ctcgcaatca gtttttggat ttttgctatt
      121 ccaattgatg attctggaga cacaaatcgg atttctctat cttcatcaag cgcgcttaaa
      181 tctgccaatt ttcaggatct tttttccatt cttttagcag agttaattct tgctcatcga
      241 tataacgatc ttctaaagct gcttcaatca atacagaata attggtcaac gtcataagag
      301 ggatttcggc tttttcgaaa tttgcttttc cttttggcaa ctcatacgta aagatagccg
      361 caacaccaag gatatccgca ccttctcttt ttgctgcttc agctgcttcc aagacacttc
      421 cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg accttcaaat atacgtcctt
      481 cgatttggtt acctttccca tgatctttcg ccttacttcg aatatagacc attggcaaat
      541 ccagtatttc cgctacccat gcggcatgag ggattcctgc tgttgcagtt ccagcaatca
      601 cttgtacttc agggtaagct tctttgattt tgcttgctaa tccttttgcg atctcttttc
      661 ggacttttgg atagctcatc gtgattcggt tatcacagta aattgggctt ttgattccac
      721 ttgcccatgt aaaaggatta cttggattta aaaatactgc ttcaatttct aataagtctt
      781 ttgcgatact ttgttcaaca ttcattttag gttagctcca ttctgtttga atcgtttgat
      841 atgctttata aggattagtt gactgagtga ttggtcgacc tataacgata aaagttgagc
      901 cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg atctcctgcg actgttcctt
      961 ctggtcttat acccggagtt aagcaaacaa attcgtctcc tgccatttct ttgatcgctt
     1021 ctacttccca agcagaagaa accacaccat ctaatcctgc ttttttggca cagctagcat
     1081 aatggatcac acttttttct aaagaaactt cgattagttg atcggcatgc atttcttctt
     1141 cacttgtaga agtaagctga gtgatagcta gaagtagcgg acgttttcca ccttctggag
     1201 tcccctcttc taatcctcgc attgccgcct ccatcatacg aataccgcca gcggcatgaa
     1261 cacaggtgag atcaacacca agctttgcca atccccgcat tgctttttcg accgtattcg
     1321 gaatatcgtg aagtttcaga tctaaaaata cattatgacc agcctctttt agataacgta
     1381 cgatttcagg tccttcttgg tagaacaatt ccattccgat tttcacaaac aatttttctt
     1441 ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct agttgaaaaa tctaatgcaa
     1501 taattggtcg ttggtctagt tgattcatcg ttcacgttcc tctctcactt ctttgattaa
     1561 tctttccagc gattcgatgc ctagttcgct cattcgcaca gggagctctt taattagttt
     1621 aggacagata tacggatcgg tgaaattcgc ggtccctata gctacagcac ttgctccagc
     1681 cataaacatc tcaagaacat catccactgt ctgcacgccg cccatcccaa tgattggaag
     1741 gctggatata cttgctactt ggtgaatcaa acgaatagcg acaggcttga ttgctggtcc
     1801 ggagagtcca cctgtttgat tggctaaaat cggttttctt gtcttaaggt caatccgcat
     1861 accaagaaga gtattgatca tcgtaaaccc atctgcccct ccagcttcga ttgcctgagc
     1921 gattggaacg atgtctgtta catttgggga caatttgaca tagacaggaa cagaagaaac
     1981 tttctttact gcttgtgtta attgaaaagc aacatcagaa tctgttccaa aagcaattcc
     2041 gccatgtttg acatttgggc aagaaatgtt cagctcgatt gctttcacat ttggtgcttc
     2101 gccaattttg ctacaaactt ctacatagtc ttcttcacaa gcaccagcaa catttgcgat
     2161 gatgggtaac tcattgtact cctctaattc tggaagaagt ttatgcataa caacgtctag
     2221 accaggattt tgcaaaccaa ttgcattcaa catgccagat ggtgtttccg ccactcttgg
     2281 cgtgggattg ccaaagcggg catttggagt cgttgccttg accatgatcg atccaagctg
     2341 attcaaatca tagtattttc cgtattcttt gccaaaaccg aaacagccgc ttgccggcat
     2401 gataggattt tttaattcta ggccaggaag tttgaccgct aaaggattct cattcatata
     2461 attacctctc ctattttaaa aatcggtcct tcgtcacaaa ctttcacact ttttgttcct
     2521 gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag cccctatccc acatgccatg
     2581 cgggattcta atgataactg gacattttga tgcgaagaaa acagctgttc cactgtcttc
     2641 aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt ccgtcttcgt tcctaacaat
     2701 aaattaccga catttccgtg cataccgtat gttccatcgt cagtagcgat tcttgtttct
     2761 cctagacgat taaattcttt ttcataataa acgacttcat gtgtggcaaa acctaaaaaa
     2821 tgcgttggct taattccttt ttcgactaac tgttttgata actcatatag aggagggaca
     2881 ccgattcctc caccgattat aaatacttcg tctcctgtat gaagattgga caaatcaaaa
     2941 ccattgccta aaggacccat gacatctaaa tggtctccgg cagaaagagt agagaagact
     3001 ctagtcccct caccttccac ccgataaatg atacggcaag tatttgtttc gtggtcgata
     3061 tgattcaagc taatcggtcg gcgtaatagc aagtctgcac gcggcacgag aatatgcaag
     3121 aactgtcctg gttgctgcat ttcttttact agatcacctt tcaatgtcat ttcaaaaatc
     3181 tttggtgcca gctgtttttg ctgaacgatt tccatcattt cttgtttcat ctgtcccctc
     3241 ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg ccgttaaata gggttagttg
     3301 aaaaagcacg ggattccatc actcgaacga tcgcatctgc tgtgtctaaa gaagtaaaca
     3361 atggaatacc atgttcaact gcttctcttc ggatgatgaa accgtcttta gaagcatttc
     3421 cccgatcttt atcaatcgta ttgacaacca cttgtgtttt accatctctt attaagtcga
     3481 ctacattttt ttctgctgct tcctcagaaa ttttcgctac tgtttcaacg accagaccat
     3541 ttttttcgaa ataagcagct gtatgtttcg ttcctatcag actatagcca atttctgtaa
     3601 aacgtttagc taattctagt gcttcttctt ttgtttcgtc cgcaatcgta aataatacgg
     3661 ctccatattc aggcaagtgt aatccagaag cttcgaatgc cttgtacaaa gctttgtcta
     3721 attgtctgtc agatcccatt acttctccag tcgatttcat ttcaggccct aagtaagtat
     3781 caactttctg caatttcgta aaagaaaaga caggtgcttt aacatgaact tcttttgttt
     3841 ctggataaag tccgttttca taaccaagtg tctttagttt ctctcccaaa atagctttcg
     3901 tcgcaatttg tgccattgga attcctgtca ccttacttaa gaatggaaca gtccgacttg
     3961 ctcgaggatt gacttcgatc acataaacct cattttcatg gatgacaaac tggatattca
     4021 tcatcccgac acaattcaaa cctaatgcta agcggatcgt gtaatcttcg atcgttttct
     4081 tgatcgattc agataatgtt tgaggtggat acacagccat tgaatcccct gaatggacac
     4141 cagcacgctc aatatgctcc atgatccctg ggatgagtac tgtttcaccg tcacaaatcg
     4201 catccacttc gcattcacca ccaagcaagt aacggtccac tagtactgga tgttctggag
     4261 atgctttcac agcatgttcc atgtaatctt ctaaatctcg ctggttttca acgatttcca
     4321 tcgcacgtcc gcctaataca tagcttggac gaacaagaac tggataacca attctcgaag
     4381 cgataaccac ggcttcttct ttacttgtag cagtatcccc taaaggttga ggaatatcca
     4441 actctttcag cgcttgctcg aataaatcac gattttccgc acgatccaag tcttcgatcg
     4501 ttgtccctaa gatttttaca ccagcagcag tcaaaggttc ggctaagttg attgctgtct
     4561 gaccaccaaa ttgaacgatt actcctatcg gctgttctag ctcgatcaca ttcatcacat
     4621 cttcgaaggt caatggctca aaatataatt tatccgaaat agaaaagtca gtcgaaactg
     4681 tttcaggatt gctgttcatg atgattgctt cgtatcctgc ctgctggatt gctttgacag
     4741 aatgcacagt cgcatagtca aattcaaccc cttgtccgat acggatagga ccagatccca
     4801 gtactagaat tgagggcttt tctgatcgtg aactttcatt ttcatattca taggaactat
     4861 aaaaataagg tgtttttgat tcgaattccg ctgcacaagt gtcaaccatc ttatatacag
     4921 gaatcaaatc attttctttt ctcatctggc gaacggcttc ttcgtttatt tcccacagct
     4981 ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt tttcaatatc ttgtcatcca
     5041 acggctgctg tttcaactgc tgttcgattt ctacgatatg caacagttta tctaaaaaga
     5101 ataaatcgat ttttgtcaaa tccgctaatt cttctatggt gtaccctctt ctaatagctt
     5161 cagctacata aaacaggcga tcatcttgtg ctcgaaccac tttttggatt aggtcatcgt
     5221 ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt accgatttct aaagaacgca
     5281 cagctttcaa taaagactct tcgatattcc gaccgatagc catcacttca ccagttgctt
     5341 tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt atcaaacggc cagcgaggaa
     5401 ttttagctac aacataatct aatgcaggtt caaattctgc aaaagtcgtt cctgtaacag
     5461 gatttttcat ttcgtctaat gttaatccaa gcgctatttt agcagctagc ttagcgatcg
     5521 gatagccagt cgccttactt gctaacgcag atgaacgaga aacacgcgga ttgacttcaa
     5581 tcacataata attaaaacta tgtggatcaa gagcgagttg gacattacag cctccctcga
     5641 tcttaagtgc tcgaataatc gaaagagaag catcacgaag catttgatat tcatggtcag
     5701 acaatgtttg actaggggca aatacgatag aatcacctgt atggataccc accgggtcaa
     5761 aattttccat attgcagaca acgattgcat tatcagccga atctctcatc acttcatatt
     5821 caatttcttt aaaacctgca atacttcgtt caatcaaaca ttgggtagcg ggggaaagac
     5881 ttaatccatt ttctgatacg atacgtaatt cttcttcatt ttcacacata cctccaccag
     5941 ttccgcctaa agtaaaggct ggacgaacga tgacaggata gccgatttta tttgcaaaat
     6001 ctacagcttc ttctactgta ttgacgattt cagactcggg gattggctga ttcaattctt
     6061 tcatcaactg tttgaataga tcacgatctt ctgcttgatc gatcgcagaa agctttgttc
     6121 ctagaagttc tatgttcaat tcttctaaaa tacctgatgc tgccagctcc attgccatat
     6181 tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc tggacgctct tttcgaagaa
     6241 tccttgatac aaattcaagt gtgatcggtt cgatatatac ttgatctgcc acttctttat
     6301 ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac ttcatatcct tcttctttta
     6361 aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc ggcttgacca atgatgatcg
     6421 gaccagatcc aatcaccata attttcttga tatctgtacg tttcggcatc tgtttactgc
     6481 tcctttcctg cgtccatcat ttccataaat tcatcaaata aatgtagtgc atcatgcgga
     6541 ccaggggcag catctggatg aaactgcaca ctgaaagcgg gataatctct gtgtttgact
     6601 ccttctactg ttccgtcatt tacttctaca tgagttacga tgagtttttc tggatcaatc
     6661 gtggcttcat cgactgcata gccatgattt tgagaagtaa aatcaattct tccggtcgct
     6721 acttctcgta ccgggtgatt taatcctcga tgtccgaatt tcattttata ggtatctgca
     6781 ccgttggcta aagcaaataa ctggtgaccg agacaaatac caaaaatcgg tacttttcct
     6841 tggatttttt tgatcatttc gattgcttct ggcacatctt ttggatcacc aggtccattc
     6901 gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct cggcatctgt gttataggga
     6961 agtactgtga ggttacagtt tcttttactc aattctcgta aaatactatg tttcaagccg
     7021 aagtcaatga caatgacttt ccgacctgtt cctggacttg gataaggttt gttagttgag
     7081 acttgctgca cttgattggt tgctagaacg gttgctttca gctgatcaaa tgcatgttcg
     7141 aaggaatctc cagcatcaac gatactagct ttcatcgttc cgacttcacg gattttgcga
     7201 gtaagggcgc gcgtatcgat acctgatatt ccaggaattc ctttttgttt caaaaatgaa
     7261 tctagcgtca tttggtttcg ccagtttgaa gctacacgtg catgttcttt tactactact
     7321 cctttgcatg ttggagaaat cgattcataa tcatctctat ttaccccata attacccacc
     7381 ataggatagg taaatgtaat gatttgaccg ttaaaacttt gatcagtgat agtttcttga
     7441 taaccagtca tacttgttgt aaaaacgatt tctccgaaga cactagcatc tgcaccaaaa
     7501 gcctttcctt cgaaaactgt tccatcttct aatataagca aacgttccat acttttattc
     7561 tcccttctgc cacacaagtt ttccgttgac aaaagtcatt tgtgtttcgc cttttacttt
     7621 ccagccgata aaaggtgtat tctccccttt tgataaaaag tcttctttat cgatcgtgca
     7681 agtttgtgta atatcaaaaa ctgctacatc agcgggggca ccaaccgtta gcgttcctgc
     7741 attcaaacca aaaatctcag ccggtttaac tgccaaccaa ttgatcactt gttccaacgt
     7801 gaatcgacca gtttctacaa aatgagtata gatcagttga aacgctgttt cgcttcctac
     7861 gataccaaat ggtgatttca tgaaactttg gcttttttct tctaatccat ggggggcatg
     7921 gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct tcgatcaaag cttctctgtc
     7981 ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg aaatcttcgg gaatatcttc
     8041 gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt acgtggattc ctgctttttt
     8101 tgcgtctctg attacacgga cactttcttt agtagaaaca tggcaaacat ggtaatgaac
     8161 accagttgct tcagctagca acagatctct agcaatttga gaagactcgg ttacgcttaa
     8221 gatcccggga agaccaagtt cttctgcctt cttgccagcg tgcataacgc cgccaaataa
     8281 taaagattca tcttctgtat gggctactag ggctttattg ttttttgcag cttctttcat
     8341 agccagatac atcgtcccag ctgtttggac acctacaccg tcatttgtga aagcaaaagc
     8401 accttcttca atcagtgctt cttgatcaac taatttctca cttcgcagat tttctgtgat
     8461 tggcgcatac tgcaatacct tcacaactgc atcttttcta atgatgtcat atacttgtct
     8521 cagcttttct gccgtatcag gtacaggatt taaattcggc atcgcgcata cagtagtgaa
     8581 accgccacga gcagctgagc gagtcccagc ttcgatcgtc tccttgtatg taaatcctgg
     8641 ctctctcaaa tgaacatgga cgtccactaa accaggtgtg atcaattgtc cctttgcatc
     8701 aaacacttca tcaaattcgg cttctgagaa tcctgtaccg attgctttga ttcgcccatc
     8761 ttctatccaa atttctgctg gtgtcgtcat attttttcgt gtattgatct gaccgttttt
     8821 aatgagtgtt ttcatttttt catcctccta taattacgct tttccattta gcactgcttc
     8881 taagattgcc attctgacga atactccatt gctcatttgc tgaacgatcc gcgactgtaa
     8941 tccttccact aatgaatcag ctaattctac atcacggtta actggtgctg ggtgcataat
     9001 gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt aacccatgtt cctgatggta
     9061 gccttctttt gagaaacttt cttttccatc atggcgttcg tgttgcaccc gaagcatcat
     9121 catgacatca acttgatcta gtagttcatc caatggcata taatgtccgt acacttcgaa
     9181 ttcatcgtcg taccattctc taggacctga gaagaatact tgtgcgccta gacgttttaa
     9241 catctgcata ttggatttag ctacgcgaga gtgagtgata tcacctacga tggcaacttt
     9301 cagaccttca aaatgaccaa attcttcata aatggtcatc aaatcaagca agcattgggt
     9361 tggatgctgt ccgcttccgt ctcctccatt gataatcgaa cactggattg ttttactttg
     9421 tatcagttcg tcataatagt tctcatcacc atgacggata accgctacgt ctacgcctaa
     9481 tgctgacatc gtcagtactg tatcgtatag tgtttcccct ttttggacag agcttgtgct
     9541 tgcttcaaat tcaatcactt ctagccctag ttttttctcc gctacatcga agcttttatg
     9601 tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag tactggcgct tttgcggagt
     9661 ccaattagca ccttgcttga attcctgccc tctgcggatc aagcccataa cttcttgatc
     9721 agtaagtgcc tcaacggtca ataaatgctt taagctgata cgttctgatt tgatgatcat
     9781 aatgtttcct ccttagtttt cttcgcttcg tgaagtttct ggtaagataa gatttaaaat
     9841 gatgccaagt acagttgcta atgccattgc cgacaatgta aatgtgccta cttcaaagac
     9901 gagtccgcca attcctacta ctaagataac tgaagcaatc aacagatttt tctttttatc
     9961 aaaatcgatt ttgttctcga ttaaaatctt cattccactt gcggcaatta ctccaaataa
    10021 gataaaactg attcctgaga tgactggtcc agggatactt aaaatcaaag cgcttaattt
    10081 tcctacgaat cctaaaatga ctgcaaatac tgcagctcca ccgatcacaa acacgctatg
    10141 gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa cttgttacag gaggacctcc
    10201 gacaaaggct gccacgattt gtgctaatcc atcacctgtc aacgtcttat gaagacccgg
    10261 ttcttcaaag aaattacgtt tagttagttt attcaatacc ataagatggc cgatatgctc
    10321 tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt gctccaagat atagctttgg
    10381 ttcatattgt acaaagggaa tctcaaagtt aggaacagca aaccaggcag cgtctacgat
    10441 tggcttagta tccacgattc caaacagcaa ggctacgatg tatccgctaa taatacctag
    10501 taaaatcgga atcaatccga gaaaacctct tagccacatg ttgaagaaaa ttgttaatcc
    10561 taatgtgatc agcgctacag caatatactt gaaatcatac gtgccctgat tatacatagc
    10621 atttgttgct gcatttgccg caagtcccaa cccgataacc atgatgactg gaccgacgac
    10681 gattggaggc aagattttat ccagccatgc tgaaccgatc tttttgatga tcagcgcaac
    10741 aatcaaatag actaatccag ttgtgactgc tccttgtgca atcgctggat aaccatctgt
    10801 tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg cttcctaagt acgcaggtat
    10861 ctttccttta gtagtaaata agtacaccaa tgtacctagt cctgaactga ctagcgcaat
    10921 acctggatcg atacctacta agatggggac taacacggtt gctccgaaca tcgtgaacaa
    10981 gtgttgtaaa cttaatccta cccaatgtcc tggggttggg cgatcgtgga tatctaacac
    11041 cacgtcatca ttatgaaatc cttttttctc cgacatgttt ctctctcctc taatcttcct
    11101 ctttcaaaat catgatgcga tcttgtccat caagctcttg catctctact aaaatctctt
    11161 ccgcccgaga tgttggaatg ttcttcccaa cataatctgc acggattggc aattctcgat
    11221 gcccacgatc gaccaggact gctaatgaaa tttttcttgg tctgccaaaa tccattactg
    11281 cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac atcatcaatc aaaataactt
    11341 cttttccttc taaggaaact gggatgtcag aagaatgaag ttccggttct tctggatttt
    11401 cttttttgtc atctcggtat aaagtgatat ccagttctcc tacaggaata tcgatatctt
    11461 ctagctgctt caggcgttcg gcaattcttg atgcaatgta gattcctcgt gttttgatac
    11521 ctaccaatac gatatcttgg atactatggt ttctctcgat aatctcataa gtaattcgag
    11581 tcaatgctcg cttcatggtt acctgatcta ctacttcttt tgcttgcatt ttcattcccc
    11641 ctactttttt gcataaaaaa actcctgccc taagaggaca ggaggaatgt ttagactgag
    11701 cgtatactga tacactgata gctatactat gtcaatgagc aatctactta tggcgcacca
    11761 tacgtatcct tgctcccacc cttcttagcc tcacgggact aaacttaaag gaattctatt
    11821 aagttttatg ctttcgttgt taattactat accgttatct aaattaaaat gcaatgtttt
    11881 ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag aggtgcttca aagatcatct
    11941 tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc atggagaaac tgaccattgc
    12001 ctttcaaagt tttcttcggt ccgtatactg gatctccagc taacggataa ccgatatact
    12061 tcatatgcac acggatctgg tgtgttcttc cggtttctag ttttaattca accagagtaa
    12121 atccttcgaa acgttccaat acagtaaaat gtgtgacagc cgacttccca tcttctctta
    12181 tcgcctgcat tttccggttt tccttcgaac ggccgattgg cgcattgatg gttccttttt
    12241 cgtgaggaat ctctccatgg actaaagcaa catatttgcg caaagaggtc ttatctttta
    12301 attgctgagc taatgattcg tgtgcctggt catttttggc aaccattagc agtccggaag
    12361 tatctttgtc gattcggtga acaatacctg gacgaataac gccgttgatc gtagaaagat
    12421 ccttgatatg gtacatcagc gcattgacca ttgtgccatg cgtatgacct gctgaaggat
    12481 gaacgaccat tccttgaggt ttgtttatca ccgctacatc ttcatcttcg taaacgatcg
    12541 tcaacgggat attttctggg accacatcta atgtttctgg ttcggggatc aagacagtaa
    12601 tctcgtctcc ttgttttact ttgtaatttg ctttgactgg ctgtccattg acagaaaccg
    12661 ctccattttt cagccacagc tgaatttgcg aacgactgta atcggtgaat aaattagtca
    12721 atactttatc gatcctacca gattcatggt gaatcgtcgt tttaagttct gtcatttttt
    12781 tctttgatcc ttttcatcta aaataatata aattaacata caaatcactc cacatacaag
    12841 tgtcacatct gctacgttaa aaatcggaaa gctcataaaa tctgtctgga acatatctac
    12901 tacatagcca agacgaatac ggtcgataaa attgccaatc gcaccagcta gaatcaaact
    12961 caatccaatc atgaaccagc gattaccttt ttcacgattt ttaatcatca atgtgagaat
    13021 cacaacgact gcaatgagtg tcactatagt aaagaaccac atcttccctt ctagcatact
    13081 ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa atacctggta taagattttt
    13141 cgtctctcca agcgctagat tatcagtgat ccaccatttg accaactgat ccactcctac
    13201 tagcaaagcg ctgaatatcc aataaataat taacaaaaaa tcaatccttt tctatttgtt
    13261 tataaatatc acgaggtcgt acgatcccga gcagattacc atcgggattc ccatctttag
    13321 tgataagtac ggcttccaaa gtcccttgct cacgaaacat cgcttcaacc tcgtaaatat
    13381 gtgttttagc tggaacaaaa tgataatttg tgtactttcc gttttggatg atcaaatcca
    13441 acgctcgttt atttgttaaa tcaaccggac ctttttggct ttcttttgct agccaaaacc
    13501 cgatattacg taaagtaatc aatgctacaa attttccttt attgtataac ggaaattgtg
    13561 aatacctttt ttctgctact atctttaaca gttcaagtaa cggaagatct tgttcaaaac
    13621 ctgttacacg ttttgcaaat cgaggcagca cggtttcagg ctgaatcagt tcttgttcga
    13681 tcagctggat tcgcttgact gtccaatcat ttggctctgc aatgacaaaa tcgactgcga
    13741 tccgatcatg gacgatcgca ttccttaact gagcaagctg taaaagatca tcttcgtatt
    13801 ttctgatcat taattgtttt ctttgagcta ggcgtctgac aagttcggta aatcccatat
    13861 ttcgtgcatt tcccatctcg tctctcatcc atttttcgat tcgattgaaa ctgattaaaa
    13921 aaagatcaga gtttgccata attttcctcc ttgatcaaat atgcccggtt ttttatatag
    13981 aaaaagaacg caatcaacac aataactgca aaaaaactga aaaataaatt ttgtaggaaa
    14041 acggccccca acccagttgc tagaaataaa aacggataga tttgaagaat caagtaattc
    14101 attgtgatca cataagcagg ctttgcttct ttcactgtat aaacggggct ttttagccaa
    14161 taaaaggtga cctgcatcgt aaagggcact tccagatcaa gcgttatctt ttgttcatga
    14221 ctgagaacac tgcgtttttc attgttgatt cttagctgaa tcgagctacc cataccgtaa
    14281 aaaccggttt ttcttataaa tgtcgttttc atcataccct cctattatgc tttagtatag
    14341 ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa caaaattaat tccctatcgt
    14401 ctaaaaaaag aagctgtagt caaaatacag ctccttcgat ggtagattat aatcaagctt
    14461 aaaacaatcc caacacttct ccgtcatttg tcacgtccat attcaatgca gcaggttttt
    14521 taggcaaacc aggcatcgtc ataacatctc cagtcaaggc tactaaaaat cctgccccta
    14581 attttgggat gatttcgcgg atcgtgatgg tgaaatcttc cggtgcacct agcagtgaag
    14641 gatcatcaga aaatgaatac tgtgtttttg ccatacaaat cggcagtttg tcccatccat
    14701 tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct gaaaacaaca ccatttcctc
    14761 cgtaaatttt cttgacgatt gtttctgtct tttcttgtat cgtttcttct aggcggtata
    14821 atggcttgta attagcttct tttctatcaa tcaatcgtac tacagctttt gctaaatcaa
    14881 cgccgccttc tgctccatct gcccatacac tcgctctctt ggcaagaatc ccctgatttt
    14941 cacacaagtg ctccaacagt gtgagttcat tgtctgtatc tgttacgaat tcattgattg
    15001 cgacgatcac aggtagctga tattgttcca tattacgtat atggcgcttt aaatttgcaa
    15061 aacctacttt caaagcatca agattctcat ttttaagttc atcttttttc attccgccat
    15121 gcattttcaa tgcacggaca gtggcgacaa tcaccacagc atccggcgct tttttcaagt
    15181 taggtacttt gatatctaaa aatttctctc cgccaagatc tgcaccaaat cctgcctctg
    15241 tgacaacgta atctgctaat ctcaatgctg tcttggtagc taaaatacta ttgcagccat
    15301 gagcgatatt ggcaaacggt cccccgtgga caaaagctgg tgttccgtaa attgtttgta
    15361 caagattcgg cttgattgca tctttcagca gtaatgccag agcgccttct gctttcaagt
    15421 ctccagcagt cacaggctga cggtcaaatg tatagccgac aacgatcctt gcaagacgag
    15481 cctttaaatc ttcaaggtct gccgcaagac acaagatagc catgatttcg ctggcaactg
    15541 tgatatcaaa cccatcttct cttggaacac cttggattgg cccacccaag ccaacaatta
    15601 ctttacgcag ttcacgatca ttgagatcga ctacccgttt ccaaatcact cggcgggcat
    15661 cgatgttcaa ttcatttcct tgatgaatat gattatctaa aagagcagat aatgcattat
    15721 tagctgttgt gatagcatgc atgtcaccag taaaatgcaa gttgatatct tccataggca
    15781 atacctgtgc atatcctccg cctgcagcac cacctttgat tcccatcaca ggaccaagtg
    15841 agggttctcg caatgcaata acagattttt tattgatttt attgagcgcg tctcctaacc
    15901 ctacagtgat cgttgacttt ccttcgccag ctggtgttgg attgatggaa gtaactaaga
    15961 tcaaatgccc ctcagcgtta tcctttgatc gattgattcc agaaaaattg attttcgcct
    16021 tatatttccc atacagttcc aaatcgtctt ccttaagatc taatttacta gccacttcaa
    16081 caatcggttc tagctctgtt tcttgtgaaa tttgcagatc attcttcatt tgattacccc
    16141 ttttcacgaa ctttattcaa caaaatatct tctttgttaa gatatgccaa ttataccttt
    16201 ttttattcag aaaaaaaaca taaaattaca ggtttttaat ttatttgacg tacttataaa
    16261 tactctttat aatgaaggct atgatcagac tttagaaagg tcgtgagtgc tttgaccaca
    16321 ggaattgtaa aatggtttga caacaaaaaa ggatacggct ttatcagtta tgatgacaca
    16381 gaagaaattt ttgtacattt tactgcgatt gaagaagagg gattcaaaac tttggaagaa
    16441 aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag gaacacaagc tgcccatgtc
    16501 aagaaaggca acgttcagac taccgatact agtatgcatg aatagataaa caaatagagt
    16561 gagacgctaa acgggatgaa ccgtacgtct cactcttttc ttttcctgta aaaattcgtt
    16621 tttttaggtt ctgattttta aaattagctt tttctttttt tcgtataagg ataaggaatt
    16681 gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa ggactacttg atcttttttt
    16741 cgcatcatga aaacagatgg ggctttctca tcttgcaaca tcatttgcac cccagtatta
    16801 gttggcgtca aagatcgaat ctgtccgaca gatatttctc ggtctttcca aaaagcagca
    16861 taacgtataa tcacagagtt tgattttaaa ataaaagaac gccgcaaacc tagaaataga
    16921 aaaacaagaa aaatacctaa aacaaaattg cttttccaat aaggacctgt gttttctaaa
    16981 gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg accaatagat gatcgtatgt
    17041 gccagttcag gctgccatcg aatatttttt ttcattctct aacctctcac cgattcagta
    17101 atgatataat cttaccataa cattagtttg aacaaaaagg agaatctctt tttatgttaa
    17161 aagtatacat tgatgcctct acaaaaggca accctggtcc tagtggaggt ggaatcttga
    17221 ttgtctatca aggtaaacaa gaacagctat ctatcccgct ttctataggg acaaaccatc
    17281 aagcagagtt cgaagtcttt ttaaaaactt tagaatttct aaagaaaaac gacttacaga
    17341 atgaaacgat tttctgtttt tcagacagca aaacactagt ttctacggtc gacaaaaacg
    17401 caacgaagaa cgagctcttc cttccctacc ttcaaagaat ccaagaactt ctatccgaat
    17461 tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa taaaggagca gataatcttg
    17521 cacgtcaggc tctccataaa caattaaaag aaaaaaagac tctttaaatt aaaaacttta
    17581 aggagtcttt ttatagtttc tcatttaata taaaagataa ttgtgatcaa acgtaatttc
    17641 caattggtaa tctccgattt tttcttctat ttgactacgt aagattcttt ctagttctgt
    17701 atcaacaaac tggaatttct cagggaccac tacatcaaat tgcagcgttt ttccttgatg
    17761 aattactcta aaatcatgaa tattaagccc tgtacctgtt tcaagaagaa tttgctttaa
    17821 ctgccgcaag atattatagt atttttcgtt atcaacaggc attggatcca agtgacagac
    17881 aagttccaca tccaaagact ttttaaagtc tttttctatc agatcaataa tcttatgagc
    17941 cacattcaaa ttcattgatt catctacttc gatgtggact gatgcaaagc gtttattggg
    18001 tccatagtta tgcaccaata aatcatgata gccaagtata tcatcgcaag catttaaatg
    18061 ctcttccatt tcttcaatct ctgctggcgt tgggcgactt cctaatagtt cgtatacaaa
    18121 atttcgaagc atcgtcaatc cactataaag gatatataat gctaaagcaa aacctatata
    18181 tccatcaata cgccatccag ttacccattc gattgctgct gaaagcaata cagccagtgt
    18241 agtaaagaca tcattataac tatctgttgc tgtagctctc aatgtttcag aatcaatgga
    18301 ttgcgcaata acctgataca ttttgccttg tgcaaatttc aaaaagatgg aaatgattag
    18361 gacaaaaaag acgatgggtg ttaaagagac attttctgga tggatgattt tttcagcaga
    18421 agactctaaa aattgaaatc ctacataagt aatgatgatc gaaacaaata acccactgat
    18481 atattcgaag cgttcgtgtc cataaggatg ttcctgatct gctggctttg cagcaatttt
    18541 aaatccgatc aaagtcaata tggatgaagc agtatctgat agactgttca ctgcatcagc
    18601 cataatggag acacttcctg aaaaaagccc gatcattagc ttagagacaa acaagatcag
    18661 attcgtaacc aatcctaaga tacccgctag tactcctttt tttgtccgct gttgcggtat
    18721 ttgtttcttt ttttcttctt gtttcttagc caagtttttt accccttcta gtttgcttgt
    18781 tgctcaggta aaaagatgga gaggacgatt gttttcctct cctctcttcc tttttctatc
    18841 tatcgtgcgt cctattacta ttaaaagatg gtcattattc tggtttcaac ggtgttgctg
    18901 caaataacgc ttttgctagt aatggtgtta tgattcccgc acctattgct tctggtatcc
    18961 cattgattgc tgcaacccct gctaaggttt taaataaaag agcagagtct acaccatagg
    19021 catctgccgt tgcacctgta taaaataatc ccatgaatcc taacactaag actgtattga
    19081 ccaatgaacc cagtacccct gcaaaggctg agctgatcgt tattgattta tttttttgat
    19141 aaatccagtg gaaaaccaag cctgctacta agccaacaag tactctggga actaccgaga
    19201 taatcggatt cgtgaacact aatgtatcaa atggcgttgt tggcatggca taggcacgaa
    19261 caattgtagc gattccccaa aacaagccaa tcaccatgcc atcttttggg cctaatacta
    19321 ctgcagcaat aattactgtg atatggataa ttgttaaact aatgaatcct aatggaataa
    19381 accccagcca aggaaccatc gcttgtacga tgatgattgc taacaagatc gcacgtaaga
    19441 ctagtcgaaa ggtcttattt ctactattca ttttttcacc cctttttatt tatactaatc
    19501 gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg aatccgacca tttgattaga
    19561 tgcgccatcc aattgaaaaa agtatgaggg caagacttgt ttgtcttcgc ccccataatc
    19621 atgtttcgtt caatgtttga acttttgttt ttctgtcaaa cgtaaacgcc ctattgttgc
    19681 gcaacccatt ctttcgcagc tttaagtgca tctggaatac cagcaggatt tttcccgcca
    19741 gcttgtgcca tgtctggacg tccaccaccg ccaccgccta ctttcggtgc gattgcttta
    19801 atcaagtcgc cagcttttag tccagcttca tttgattctt tcgtcattgc cgctagcaga
    19861 ctgacttttc cttcttgttc gtttgctaaa acaagaatat ctgaaatatt tttttgtttc
    19921 cattggtcag ctaattgacg cagctgattc atatctttca cttttacttg tgctgcaatg
    19981 attgtttttc cattgatcgt ttctggatct ttgaatacat caccagcttg ttgattcgct
    20041 aacttgctag ctaattgttc attttccttt tgtagctctc ttacttgctg ttgcaattgt
    20101 tccgcttttg aaacaacttc tttcaattga ggtgatttaa caattgttgc tacttgtttt
    20161 agttgttttt cttcttcttc aagtagttcg tacgcttctt tacttgttac agcctcgatt
    20221 cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt tgaagattcc gatgttttct
    20281 gtatttgcta catgtgtgcc tccgcacagt tcgattgacc agccgccaat atttactact
    20341 cgaacgtcat gtccgtattt ttcaccaaac aaagccattg cacccatgtt tttggctgta
    20401 tcgatatctg tttcaattgt ttcaactggg atagcttccc agattttttc gttgacgatt
    20461 ctttccattt cagctaattc atctgaagtg atttgaccaa agtgggtaaa gtcgaatcgt
    20521 aaatgtccag gagcgactaa agaaccagct tgatttgcat gctcgccaag tacttctttc
    20581 aatgccttgt gcagtagatg ggttgctgta tggtttttga tgatccgatt gcgcatcgat
    20641 tgatcgatca atagatgata ggtttgtcct tcactcattg gtgcttttac ttctacggtg
    20701 tgcataaatt gaccattagg cgctttttga acatctgtaa caactgctac gatttcgcct
    20761 acttcattca tgatccatcc ttgatccgct acttgcccac ccatttctgc atagaaaggt
    20821 gtttgatcaa agatcatttg agcttcacct tctgcgactt cattcaaaag agactcacct
    20881 ttaacgatga ccaataattg actatctgat tcttgttctg tgtatccaat gaatttactt
    20941 tctactttga tatctgtcaa taaagcagat tgtaccccca ttgatttttc agtactgcgg
    21001 gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt caaaaccagc atgatcaact
    21061 ttcagtcctt catcttctgc cacctcttcc gttaattcta ccgggaagcc atatgtgtcg
    21121 tatagtttga agatgtcttt tcctgctaga gtcgtcccgt tttcgttttt tacttcttgg
    21181 atcacttgat ttaagatttc caaaccttca ttgattgttt cattgaaacg ttcttcttct
    21241 gtacggataa ctttttcaat aaattctttt tgttgaagta cttcaggata ataacttacc
    21301 atgatttccc caacaacagg gactaaacga tataagaatg cttgttcaat acctaatttt
    21361 ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga cataccctct cccttcattc
    21421 gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc gaatatgatc tgcgattact
    21481 ttgtaagaaa tatctgttac tgggttttca ccatatttta cttgtttgct tagcttttct
    21541 acggcgtgga taataggcat aaacaagtct gtctcaaagt tcgttgacgc atcttgaacg
    21601 attgagacca tgcgttccaa tcccatcccc gtatcaatgt ttttatgagg aagtggttca
    21661 aatgtgttat caggcttatg gttgaattct gaaaatacta agttccagat ttctaaataa
    21721 cgttcatttt ctcctcctgg atagttttca ggatcgtctt ctgctaaatc gttataagct
    21781 tctccacggt catagaaaat ttccgtatcc ggaccacatg ggcctgcacc gatatcccag
    21841 aagttgtctt ctacatcaat aatgtgatct agtggcaggc caacttcttc atgccagatg
    21901 cgttttgctt ctgtatcttt tggataaact gttacataaa gtttttctgg atcgaatgcc
    21961 atccattttg gatcagtcaa aaattcccat gcccagtgga tggcttcatt tttgaaatag
    22021 tcaccaatcg aaaaattacc cagcatctca aacatcgtat ggtgccgtgc tgttttaccg
    22081 acattttcga tatcatttgt tcgaatagat ttttgggcat ttgtgattct tggattttct
    22141 ggcacaactg aaccatcaaa atattttttc aatgttgcaa caccagagtt gatccacaat
    22201 aaggttggat cgttcacggg gatcaaagac gcactaggtt caatcgtatg tcctttagat
    22261 tggaagaaat caagaaacat ttgtcgaact tgtgcactac ttaattgttt catgtcatta
    22321 cctgctttct ataaattaaa tacaaaaaaa caccatcaca gccaaggacg aaaactcgcg
    22381 gtaccacctt gattgcaatg atgtgtaatc attacctctt aaactcgata acgctgagct
    22441 gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt gattagtgac tattcttttt
    22501 tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa tcatatcctt ttcgtactta
    22561 attagtatac gcattctatt gatcactgtc aatatctcaa tttttatttt catttccttt
    22621 tggtcatact ccacgatttt tggtacgata agaataaagg aatcaataca tctctgctat
    22681 tcatgacaag gaggaatatg atgaaaaaaa tagttggagc ggatgaatgg atacgcgcag
    22741 ctagctgctt tttgagatat caagtttttg tattagaaca agggattcct gcagaactag
    22801 aattcgacgg aaaagatatc ccgcaacaaa cctattatct atttcttgat caaaaaagac
    22861 cagtagctac tttgcgttat gaaaagaaaa cacctacttg cttcaagccg gaacgatttt
    22921 gcgtagcaaa agaataccga ggaaaagggt taggcggtca gttgcttgaa gctgctgaaa
    22981 aacgggcggt ttcagaaggt tgtcagacta gttatgtagt agctgaaatg acggctgtag
    23041 atttttatga aaaacaaggg tatcatactt gcacagaacc ttttattgaa gatgaagtac
    23101 tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga gattgtgaca cgccttttgt
    23161 cacaacctct tgttttttat ttatgtgaat ttttcttatt tttacgagca gtacggcttt
    23221 gctgtctgcg ttcgattttg cgttcttttt tatttttttc tgcaattgcc caatcgattt
    23281 ttttcttata accaggcttg atctttttct ttttcttctt cacaagacca atcagcgtgg
    23341 gatctaatac atcttttgat ttttctcgtt tggcacgtct attacgatcg tatgttttga
    23401 cgatttcccc gtttttgatt tcttttggtt caaatgagac gcccaatttt tctatcgcat
    23461 caatggcttg ctcatcattt ggcgaatata acgtaatggc cgttcctttt aagccattac
    23521 gtccagtacg tccgacacga tggataaaga agtctagatc ttctggaatt tctgcattaa
    23581 taacatgaga gacaccttcg atatcgatcc ctctagcagc taaatcagtc gctacaacat
    23641 attgatactc cagattctgt acttgtcgca tcactcgttt tctctctctt ggggtgatgt
    23701 ctccatgaat ctttgctact ttcaagcctt ggtttttcaa gtaatcggca atttcatcca
    23761 catgctgttt tgtattggca aatacgattg ctaaataagg atgaccaatt gtcagcagtt
    23821 gataaatcat ttgattctca tctttacctt ttgttgaaat cagccaattt tcaatgtctt
    23881 cagaaataac tgcttttggt ttgatttctt caatgattgg attttccatg tattttcgta
    23941 agaatggacg aagtttttct ggaatagtgg cagaaaaaac taaaaactgc agcttttctg
    24001 gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc catatccaat gtcatatccg
    24061 cttcatcgac aacaaatgca aatgcggtgt gaatcttcag cgcttgttca ttcatcatgt
    24121 ctaatattct accaggcgtg cctactacaa cctgtggctg ttggtgatgc agacgattta
    24181 attgacgctg tttgtctgta ccgccgacaa aattcgagac acggatctcc ggtgtagaaa
    24241 atttagcgat ttgtgtagct gcttcataga tctgtgcagc tagttcccta cttggagctg
    24301 taataacaac ttgtacttcc tctttttctg cattgatttt ttcaaacaaa ggcaacaaaa
    24361 acgtatgtgt ttttcctgta ccagtttgag attgcccaat gatatttttt ccttttgcaa
    24421 ttaaaggaat caaacgttcc tggacctctg tgggctgttt gaagcccttt gcggttaacg
    24481 cttcattgat aaacggtttg aagcggaata tatcaaaatt attcattcgt gcacctcttc
    24541 tctttaaatt atcacgactt tggcattata acacagacaa agcgtttttg ctcagctaat
    24601 ttcgagaatc atcatccttt gtttggccaa tcgtgctaac agtatatgtg tgactagctt
    24661 tttcataaaa cagaatccgt ttttttctca aaaatgatag caagatattt aaaataaaaa
    24721 taccttgtag taacaagaca ccgagagaaa tgaatactgc actcatctgc aacaccgtat
    24781 tcgttgtcga attcatgatt tgctgaagca actgccccaa ccagcttaaa ccataaaaaa
    24841 gaaagtacag atacccgtag cggatcatca atgcttttaa agaaattcgt tcgcggtctt
    24901 cttccaccaa acggatacgc acaatttttt tgccaagtgt ccatccattg gttataaacg
    24961 gtacaatcat aaagtatacc cctacttcta ataaaagcca ccagatatga gaagtaactg
    25021 cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac caaattaata agagataaaa
    25081 atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa tgagacagtc tttccttttt
    25141 cataagaaag ttcatccatt cgtcttcttg taggaagcat aaatgtaaat aacggcgtaa
    25201 cgacaaaacc aatgattcca cccaatgtat ttgtcactaa atcattcaca tcaaataagc
    25261 ggtatggtct tgggtagata aaataaagtc cacttagctg cgtcaattca aaaaatagag
    25321 aaaggcagaa actagcaatc atcgttttca taaaagaaag ccggaaataa tatcttaaat
    25381 aaatgccaaa tggtatgagt aataaaacat taaaagctgg ttccaaaaat acactctgct
    25441 tcattgccgg caggtaggta ctaggttcag acaagaccaa taccgtgttg tctctgaaat
    25501 gttgccatga agcaaataaa tgcagctcca tctttggccc tgtcatttgg gcaaccgtct
    25561 cctttgccgg aagtggtaag atcaccaaaa aatacgcaca caataaataa aaaacgaaag
    25621 aatacaaaat aactgccctt gttactagaa atgttccata ctttcgatat tcataaataa
    25681 aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac aatcagcgca aaacgaatcg
    25741 gttcgatata acttgccata tgactcctcc ttgattcttt gtatctctct tatcttagca
    25801 gtcctttgct gtttcaagca agtaacgagg ctattatttc tcatttcttt caaagtaaag
    25861 taagataaac acaaaggggg tgtcgagatg ctgaatatgt actttgtttt tggcgtacct
    25921 atttttttac tgttccttta tgctacgatc gcttatgtaa gaaaaagaac tacaattcat
    25981 tatttaggat ttattctcct gattatttcg ggtttcatgc tagtcttcaa tcttcagact
    26041 tggcagcaag cacttcttga aatggataaa atgactccac atgctttgtc taaagtgctt
    26101 ggctaccctg tctatctgat ttggcttccc attttcattt ctggttgctt ggttttatta
    26161 aatatttatc gcggcgtacg ccgaatagtt cagttacgca aatcaaagta aaagacagaa
    26221 atcggttgag cccgatttct gtcttttact ttgctatttc atcgtagaca aaacgacatt
    26281 tacgtgatca aaattaacga gtacttttga catttcttcc acgtactcct catttaaata
    26341 aaatattttg tttggcagag aagtgcagtt ctaataaaat tcgaatgttt tagctatcta
    26401 cgttttaaat agagataaat cgggatacca gcaagtgtca atactatccc catcgaagct
    26461 aaaaaggtct gagtgataag cgtgcttgat acgataaaag tccctccaat gattgctact
    26521 agtggaataa acggatacat tggaactttg tatggtcgaa acaaatcagg ttctttttta
    26581 cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa tccaaatgac aaagatcagc
    26641 atatccgtta gtgtatcaaa accaccgatc atcatcatgc caatagcgat gactagctct
    26701 aagatacctg cggctacagg aactttagtt ttatcatgaa gtttagcaaa gagcttgcta
    26761 aaaggcaggt tgttttcttt tgcaataaca taagggagac gcattccagt aagggtatag
    26821 ccattgatgg ttccataaac agaaatcaag ataccaactg tgaccagtct tccaccgaag
    26881 ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc tgtttcctgc gactccgtcg
    26941 atagatgcgg ttcttaagaa caccgcattt actaataaat aaacgatcat gattccgatg
    27001 attcctaaag aaattgcttt tggcaaatct ttcgccggct ttttcaattc tcccgaaata
    27061 tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg ctagcaagcc cgctcccaag
    27121 gctgagaaaa atgaaagatt ttctcctgct tggatcggaa acagttggaa gtctactcct
    27181 tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag gaataagctt gcagacaagc
    27241 gtaatcgact gaaaagcccc tccagcttta gaacctaaaa aattgatcaa caggatcgat
    27301 acagcagctg taacagctac gggaacaatc attgactggg aaagaccaaa tagattgacg
    27361 aactgtgtac cgaaaatgat cgacaaggct gctacatttg ctggaaaata aatcacgact
    27421 tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa tccgttcgat atatttaatc
    27481 atccctcctg tttctggaat agcagctgcc agttcagctc ctgttagtcc tgcacagaca
    27541 gaaatcatac caccgagaaa ccaagaaaac atatgcaaac tggcggatcc tgtaacttct
    27601 gctacgcttg cggctttaaa aaatacccct gcaccgatca ctgtgcccat aacagtcgat
    27661 aaagcagtaa aaaatcccat cgtacgtttt aattcatttt ctatttttac ttcttttact
    27721 ggcgtggctt gatattcttc catcccttgt cccctttcat accatagcca atcccaaaag
    27781 tataaaaaac tttttggatg tggttcttgt tttataccgt ctctaccaat ttttctttca
    27841 gtttttccaa catatccttt gtcatcacat ctaagtcgta tttcggcgaa aagccccatt
    27901 cctcttgcgc acaagaaaca tctagtgaat ttggccaaga ttctgctatt gactgacgta
    27961 caggatccac atcataatcc atttcaaaat ctggtatgat tttttgaata gatgctttga
    28021 tctcttccgg gtcaaaagac atcgccgata tattgaaggc atttcgatga accaatcgat
    28081 caggatctgc attcatgagt tttataatgg catcgatcgc atctggcata tacatcatat
    28141 ccattgatgt accttgatca atgaaactag tgtactttcc ttctttgatt gctgaataat
    28201 aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa ggttgcataa gaaatcaatc
    28261 cagggaaacg gacaccacga gtatctacgc catatttcac ataataataa tcacatagta
    28321 gttctccagc taccttcgtt accccataca tggttgtagg acgctgaaga gtatcttgtg
    28381 gggtatgatc ttttggagta tttggaccaa acgcgccgat tgaacttggc gtaaaaaatt
    28441 tcagctggaa ttctctagcg acttccaatg cattgaccag cccagtcacg ttcaaattcc
    28501 aggctaattg tggttttgct tctgcaacag ctgacaaaag agcagccaaa tgaatcaatg
    28561 tgtccacttg atattgttct gctatgctgc gcattttttc gtaatccatt acatctagga
    28621 tctcaaagat atcgtttttc tcaatgactt caggcatgcg tatatctgtc gcaatgacat
    28681 tttcactgcc tagttcttga cgtagccgtg tgatcaactc tgaaccaatt tgtcctaaac
    28741 atcctgtcac caatactttt ttcatgatga tcctccctga tcttttttac tactatcttc
    28801 agatgattaa caatatatgc gtgaacatgt tctctaattt taaatagacg aaaaaactaa
    28861 atttagttaa ataatttcca tttcttttcc tattttttga taaactgcta ctgcttcatc
    28921 aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg atcctgcctg tcccaagagg
    28981 cacagttgga taaacgattg gtttagcata aatcccatgt tcgatcagct tcttagagaa
    29041 agtctgagtc actttttcgt ctcccaaaat gactggagta ataggtgttt cagacatacc
    29101 gatgttatag cctacttttt tcaattcttc tttaaaataa ttagcatttt cccaaacttt
    29161 ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa gccaatgcag ctgcagctgc
    29221 acctggagtt agcgaagtag aaaataggaa tggccgactt ctagctttca accagtcgat
    29281 tagtgttttc gatccagcta cataaccccc aacaacacct atagctttcg ataacgtgcc
    29341 catttgcata tcgattttgt cgcttaatcc aaaatgcttg acggttcctg caccttttcc
    29401 agttacgcct gagccgtgcg catcatcgac gtaggtgatc agtccatatt tttcaacaat
    29461 cggaataatt tccggcaatc gtgcaatgtc tccatccatg gagaaaacac catctgtgat
    29521 atacatgatt tttttatatt tcttactttc tatcgcttcc tttgcttttt tctcaaggtc
    29581 tttcatatct tgatgtttaa ttcggatgat ttttgctccg gataagcggc aaccgtcaat
    29641 aatagatgca tggtttaatt catccgataa gatagcatct tcttttgtca ttactgcaga
    29701 gatagcgccc atattgcagt tgaatccaga ttgaaaggcg attgctgctt ctgtcccttt
    29761 gaattctgcg attttttctt ctaattgctg atggattttc agcgaaccat tgatcgttcg
    29821 gactgctcct gctcctacac cataagtttc ggttgcttca atacatgctt ttttcaattc
    29881 ttcgcgattg gcaaatccaa gataattgtt ggatgctaga ttgatcatct tttttccgtc
    29941 tacgatgata caagcaccat tttcactttc tagtatgtcg atcgtattgt ataacccctt
    30001 ttctttcagt tgatccaatt ctttctctaa atacattgtt agttctttgg aagacattgt
    30061 ctccacctcc atattcatct gtcttgcaga acataccaaa catatatcgt gtcattcatc
    30121 tacaaagcaa gtatgctata tttttgataa cgcttacact gattatgaga attatttaac
    30181 cacatttttt atttacagta tctttttact ttcaaatgac ttatatagta tgaacaatta
    30241 ccttttagat aatgatctat tttactctac ctgggcagat aaatacaaca caaccattga
    30301 tagaagccct ttcgttaaaa tcaattgtat tctgaatatt taatttattt attgcataga
    30361 ccaat
//
LOCUS       DAJSNV010000022        30224 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSNV010000022 DAJSNV010000000
VERSION     DAJSNV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.023"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            48..866
                     /gene="purR"
                     /locus_tag="ON516_001023"
     CDS             48..866
                     /gene="purR"
                     /locus_tag="ON516_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3344517.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1369..2742
                     /gene="glmU"
                     /locus_tag="ON516_001024"
     CDS             1369..2742
                     /gene="glmU"
                     /locus_tag="ON516_001024"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3344518.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2836..3807
                     /locus_tag="ON516_001025"
     CDS             2836..3807
                     /locus_tag="ON516_001025"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3344519.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3813..3915
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3981..4550
                     /gene="thiT"
                     /locus_tag="ON516_001026"
     CDS             3981..4550
                     /gene="thiT"
                     /locus_tag="ON516_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3344520.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4631..5404)
                     /locus_tag="ON516_001027"
     CDS             complement(4631..5404)
                     /locus_tag="ON516_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344521.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5671..7371
                     /locus_tag="ON516_001028"
     CDS             5671..7371
                     /locus_tag="ON516_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3344522.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7341..7559
                     /locus_tag="ON516_001029"
     CDS             7341..7559
                     /locus_tag="ON516_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3344523.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7579..8469
                     /locus_tag="ON516_001030"
     CDS             7579..8469
                     /locus_tag="ON516_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3344524.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8487..8771
                     /locus_tag="ON516_001031"
     CDS             8487..8771
                     /locus_tag="ON516_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344525.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9258..9872
                     /gene="gmk"
                     /locus_tag="ON516_001032"
     CDS             9258..9872
                     /gene="gmk"
                     /locus_tag="ON516_001032"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3344526.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9877..10182
                     /locus_tag="ON516_001033"
     CDS             9877..10182
                     /locus_tag="ON516_001033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3344527.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10435..12843
                     /gene="priA"
                     /locus_tag="ON516_001034"
     CDS             10435..12843
                     /gene="priA"
                     /locus_tag="ON516_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3344528.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12858..13349
                     /gene="def"
                     /locus_tag="ON516_001035"
     CDS             12858..13349
                     /gene="def"
                     /locus_tag="ON516_001035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3344529.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13342..14280
                     /locus_tag="ON516_001036"
     CDS             13342..14280
                     /locus_tag="ON516_001036"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3344530.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14280..15638
                     /gene="rsmB"
                     /locus_tag="ON516_001037"
     CDS             14280..15638
                     /gene="rsmB"
                     /locus_tag="ON516_001037"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3344531.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15651..16391
                     /locus_tag="ON516_001038"
     CDS             15651..16391
                     /locus_tag="ON516_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3344532.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16388..18457
                     /gene="pknB"
                     /locus_tag="ON516_001039"
     CDS             16388..18457
                     /gene="pknB"
                     /locus_tag="ON516_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3344533.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18622..19521
                     /gene="rsgA"
                     /locus_tag="ON516_001040"
     CDS             18622..19521
                     /gene="rsgA"
                     /locus_tag="ON516_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3344534.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19534..20184
                     /locus_tag="ON516_001041"
     CDS             19534..20184
                     /locus_tag="ON516_001041"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3344535.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20185..20823
                     /locus_tag="ON516_001042"
     CDS             20185..20823
                     /locus_tag="ON516_001042"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3344536.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20978..21832
                     /gene="mreC"
                     /locus_tag="ON516_001043"
     CDS             20978..21832
                     /gene="mreC"
                     /locus_tag="ON516_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3344537.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21836..22345
                     /gene="mreD"
                     /locus_tag="ON516_001044"
     CDS             21836..22345
                     /gene="mreD"
                     /locus_tag="ON516_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3344538.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22600..24174
                     /locus_tag="ON516_001045"
     CDS             22600..24174
                     /locus_tag="ON516_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3344539.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(24242..26041)
                     /locus_tag="ON516_001046"
     CDS             complement(24242..26041)
                     /locus_tag="ON516_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3344540.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            26164..26670
                     /locus_tag="ON516_001047"
     CDS             26164..26670
                     /locus_tag="ON516_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3344541.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            26749..27471
                     /locus_tag="ON516_001048"
     CDS             26749..27471
                     /locus_tag="ON516_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3344542.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(27549..28949)
                     /locus_tag="ON516_001049"
     CDS             complement(27549..28949)
                     /locus_tag="ON516_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3344543.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(29119..30093)
                     /locus_tag="ON516_001050"
     CDS             complement(29119..30093)
                     /locus_tag="ON516_001050"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3344544.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         9843 a         5530 c         6429 g         8422 t
ORIGIN      
        1 cttatataat gtaaaacgat aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc
       61 gcagtgaacg tctagttgac atgacacagt atttattgaa tcacccgcat gaattgattt
      121 ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc ttctatcagt gaagatttgg
      181 gaatcatcaa gaaaacattt aaagaacgag ggtatggaac cttggaaaca atcccgggtg
      241 ctgcaggtgg tgtattgttc atccctgaaa tttcatatga agaggcgaaa aaatatattg
      301 aatctctagc agaacgtcta tcagaacaag accgtctgct tccaggagga tatgtttatc
      361 tctctgattt gttaggagaa cctgaactgc tgcgtcaagt tggaagaatc attgcttcta
      421 aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc cacaaaaggc gtacctattg
      481 cccaagctgt gtcctattat ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa
      541 taacagaagg atcaacagta agtgtcaact atgtgtctgg atcttctgaa agaattgaaa
      601 agatggaact gtctaagaga agtttgaaac gtggttcacg tgtccttgta gtggatgatt
      661 tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag
      721 aacttgttgg catcactgta tttgccgaat caaaattcaa cggtcatcgc gccatcgatg
      781 attatacgtc actgctttat gtcaaagatg tagatactca aacgaaaaat attacggttg
      841 tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa gccgcttagt ttcgagtagt
      901 aagaaaaatt ttgtgaaaat ggctttccac attttttcat aaatttggct taataccgag
      961 aaactggctt ttgaacaccg tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta
     1021 ttagctcaat aaacggaaaa attgctcctc atattttgcc gtttattgag ctaataccga
     1081 aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa gcctaccgtt taactctaag
     1141 tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta tgagtagtgt gacttgtgac
     1201 cgaggagttg gcaggagagc accggttacc aaatagatag gcccgttatg gtctatctat
     1261 ttttttatcc agaaaacgct gggataaaag aaaatcactt gttttgttga aaaaatgggt
     1321 atactgggat aaactgaaat cagcaaacta tatttaggag tgacgcagtt ggacgcacgt
     1381 tatgcaatta ttttggcggc agggaaaggt acccgcatga agtcaaaatt atataaagtt
     1441 ttacatcccg tttctggtaa acctatggta gaacatatca ttaatcgggt cagtgaaacg
     1501 aaaccagatg aagtgattac gatcgttgga cacggagcag aacaagttaa agcacaacta
     1561 ggagaacgca gtaaatatgc gttacaagca gaacaattgg gaacaggaca tgctgttttg
     1621 caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt tagttatcag tggcgataca
     1681 ccattattga ctactgaaac gctgaacaat ctttttgaat atcaccaagg aaaaaatgca
     1741 agtgcaacga tcttgacggc acaagcagaa aacccaacag gatatggacg aatcatccgt
     1801 gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct
     1861 cttgtacaag aaatcaatac aggaacttac tgttttgaca acgaagcttt gttcgatgcg
     1921 ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact atctaacaga tatcatcgaa
     1981 atcttaaaag aagaaggcca tacagttgct gcttatcaaa cggatgattt tgaagaatca
     2041 atgggagtca atgatcggat cgcgttagct aaagcaaatg agatcatgag aaagcgaatt
     2101 aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt cagcaacgac atacatcgat
     2161 gcaggcgtag aaatcggtcc agacacattg atcgaagcag gtgtacaaat ccaaggaaat
     2221 acagtcattg gttctgattg tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt
     2281 gaggatcacg tcgtcattga aaactcagtc attgaaagca gccatgtcaa gaaacatgca
     2341 gatgtgggac cttatgcaca tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc
     2401 ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag gaactaaagt cggtcacttg
     2461 acttatgttg gtgatgctac gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc
     2521 gtcaactatg atggaaagaa caaacatcat acgactgtcg gcgaccacag ctttattggt
     2581 tctagcacga atatcatcgg acctgtcgag gtagctaaaa acagttcgat tgctgccggg
     2641 tcaacaatta cagacaatat tccagaatat gcgctggcaa ttgcgagagc aagacaagtc
     2701 aataaagaag gatatgcaaa aaaactccct tatctaaact aagacttttg caaaatcact
     2761 tgatttattt aaaaaatatg actactattt attaggtaga attctagagt tgtgaaaaag
     2821 aaagtggagg tccccatgtc aaaacattac tttgacccaa gattaaaaat ttttgcgtta
     2881 aattccaatc gcccattagc agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa
     2941 tcatctgtta ctcaatttag tgatggagaa attcaagtaa acattgaaga aagtattcgt
     3001 ggatcgcatg tctatgttat tcaatcaacg agcagcccag tcaatgataa cttgatggag
     3061 ttgctgatca tgattgacgc actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg
     3121 ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg
     3181 aaattagtag caaacatgat tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt
     3241 catgcagtcc aaatccaagg tttctttgat attccagtcg atcatttgat gggtgcgcca
     3301 cttattgcca attatttctt agaaaaagga atcaaaggcg atgatgttgt cgttgtttca
     3361 ccagaccatg gtggtgtcac acgggcacgt aaattagcgg aatttttgaa atctccgatt
     3421 gcgatcatag acaaacgccg tccaaaagca aatgttgcag aagtaatgaa tatcattgga
     3481 caagtcaaag gcaaaacttg tgtactgatc gatgatatga tcgatacagc aggtacgatc
     3541 acattggctg ccaatgcttt gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca
     3601 catccagtat tatccggccc tgctttacaa agaatagaag attcagctat cgagcgtcta
     3661 gttgttacag actcgatcta tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta
     3721 agtgttggcg gactaatggg agatgcaatc aaacgtatcc atgaaaacaa accagttagt
     3781 ccattgtttg aaacaaaaaa taaataataa ataaactcac aaaggggagt ccaattgggc
     3841 tgagattgaa tctatttcta aacccttcgt acctgtatcg gttatgcgag cgtaggaatt
     3901 gtgaataaac agctgtttga ctattttcga aagctgcttc ctcctttgtg acttttttat
     3961 tcaaaggagg aagttttttt atgggaaaaa atagagtttg ggtagaagga acgattgttg
     4021 cagcgttagc gatggtgttg tcgttgattc cgatccagat cggcagcagt ttttctattt
     4081 cattagggca gattccattg actctttttg cgttaagaag agggacaaag gcagggatgc
     4141 tggcaggttt catttggggc atcctacatt ttccgttggg acaagtttat tatttaagtg
     4201 tcgttcaagt gttgattgag tacccgattg cctatacttt tgcaggcgca gcagggctca
     4261 tggcttcaaa tgtccaaaaa ggctatgtag acgataatac gaaacaaatt agaaaaagca
     4321 tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt ttggcatttt attgcaggag
     4381 tggttttctg gggagcgtat gcactatggg ggatgaatcc ttggctcttt tctttcgtaa
     4441 tgaatggtgc cagcgggttg agtacagcta ttgtaacgat catcgtttta ttagggatcc
     4501 aacaaacgac tccttcgatt tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga
     4561 agcaagaaac ttgagacaga atcatgtcct agtttcttgt ttccttttta ttttttgata
     4621 agaaatagat ctacttcaat ggcagataag aaagatcggc atcttctacc aattgccagt
     4681 gtcgatcggt gacagagtag attaaagtag cgatgatttc ctcttctcga taccgggtgc
     4741 cttttaattt ccaaatataa tcttcaatca catagattgg gatacgatat ttaccatcga
     4801 tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg catcaaatta cgcatttctt
     4861 tcaataattc cacactgatt ttttcaacag gtgtttcttt tttgaaaaaa gattttttta
     4921 cattgatctt atctgacaag atcgaactta atttactagc aaaaatccta taaaattcat
     4981 ctaaatagcg ataataaatc cgattcagct gatcattaag atcgaagtat acaaaattat
     5041 tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca aaatataata
     5101 attctgaaat ttctaaaggt gtcaattctt tcagcatcgt taaatcagta aagtcgatcc
     5161 acttcaattc atcgaatcca cgcctgttac gagaagagga aaagaaacgc tgaatatttt
     5221 ctgtaccacg gattactttc agccctgtat gggattcata ttctccagca gaagcagatg
     5281 gatcaagcaa gagcaagttt tgaggaaaat gcacgatact gtttgcaaag tcagtagcag
     5341 tcagcccttt tgttaaaaca gcattacttg tggtatcaat atgtacataa actgactcag
     5401 tcatcatgtt cctcctctta tttcctctta atctctttta ttttacaaca tttgcattaa
     5461 aaaagcttgt ttcttttatt ttaaaattcc attacaaaat cacgttttca ttgaaaacac
     5521 tctcattttc aattaatttt tgtgattttt gtattttttt atttaaagga ggttttatga
     5581 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
     5641 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
     5701 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
     5761 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
     5821 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
     5881 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
     5941 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
     6001 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
     6061 aagctggtct atcatacagc acttgtttta ggtggaggag gagcgcacgg tgcttatcag
     6121 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
     6181 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
     6241 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
     6301 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
     6361 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
     6421 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
     6481 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
     6541 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
     6601 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
     6661 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
     6721 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
     6781 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
     6841 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
     6901 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
     6961 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
     7021 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
     7081 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
     7141 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
     7201 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
     7261 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
     7321 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
     7381 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
     7441 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
     7501 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
     7561 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
     7621 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
     7681 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
     7741 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
     7801 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
     7861 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
     7921 gtgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
     7981 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
     8041 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
     8101 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
     8161 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
     8221 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
     8281 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
     8341 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
     8401 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
     8461 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
     8521 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
     8581 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
     8641 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
     8701 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
     8761 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataaacagaa ttggatttct
     8821 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
     8881 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt
     8941 tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg
     9001 cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa
     9061 ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac
     9121 agctttttta cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa
     9181 agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta
     9241 gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg
     9301 gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt
     9361 attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt
     9421 tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg
     9481 cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg
     9541 aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag
     9601 tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga
     9661 tcgtaggccg aggaacagat gcagatgaag taatcgaaaa acgaatgaaa gttgcaaaag
     9721 aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag
     9781 cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg
     9841 gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac
     9901 tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg
     9961 catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc
    10021 ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta
    10081 aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa
    10141 cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga
    10201 acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc
    10261 tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt
    10321 aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg
    10381 caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca
    10441 ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat
    10501 ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt
    10561 ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca
    10621 gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa
    10681 gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt
    10741 ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg
    10801 gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa
    10861 gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat
    10921 atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg
    10981 ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa
    11041 aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa
    11101 aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt
    11161 atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca
    11221 ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag
    11281 caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat
    11341 cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa
    11401 atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg
    11461 gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg
    11521 gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc
    11581 gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt
    11641 tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta
    11701 ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta
    11761 ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat
    11821 atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa
    11881 atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat
    11941 tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc
    12001 tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa
    12061 cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt
    12121 actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg
    12181 gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca
    12241 aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa
    12301 gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct
    12361 agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa
    12421 gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa
    12481 aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat
    12541 cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca
    12601 aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta
    12661 ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc
    12721 atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc
    12781 cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt
    12841 taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa
    12901 gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat
    12961 atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca
    13021 agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa
    13081 tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct
    13141 atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag
    13201 agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact
    13261 tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag
    13321 aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct
    13381 gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag
    13441 cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa aaaagcggct
    13501 ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa
    13561 gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct
    13621 gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct
    13681 aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt
    13741 gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc
    13801 ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga
    13861 agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa
    13921 gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg
    13981 cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca
    14041 ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact
    14101 acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc
    14161 ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc
    14221 gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa
    14281 tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag
    14341 agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa
    14401 gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc
    14461 atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg
    14521 actgggtcaa gatgcttctt tatctttctg tataccaatt agaattttta gataaagtcc
    14581 ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga
    14641 cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc
    14701 aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga
    14761 cgaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat
    14821 atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca
    14881 tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta
    14941 tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga
    15001 tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg
    15061 tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc
    15121 cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg
    15181 aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta
    15241 gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt
    15301 gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg
    15361 aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa
    15421 agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg
    15481 ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc
    15541 ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca
    15601 cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg
    15661 aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta
    15721 ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg
    15781 gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag
    15841 ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc
    15901 tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag
    15961 cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt
    16021 tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag
    16081 tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa
    16141 cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac
    16201 taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa
    16261 taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc
    16321 gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg
    16381 acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat
    16441 cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt
    16501 agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca
    16561 gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt
    16621 tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa
    16681 acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca
    16741 gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc
    16801 ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat
    16861 cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct
    16921 gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg
    16981 gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac
    17041 aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt
    17101 gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta
    17161 taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa
    17221 cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac
    17281 ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga
    17341 aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa
    17401 aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt
    17461 ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc
    17521 ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat
    17581 tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac
    17641 agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt
    17701 agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt
    17761 ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat
    17821 catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt
    17881 cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga
    17941 aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta
    18001 tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga
    18061 agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt
    18121 gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc
    18181 agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat
    18241 tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta
    18301 ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag
    18361 cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac
    18421 aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa
    18481 gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat
    18541 tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg
    18601 gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc
    18661 ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc
    18721 cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa
    18781 acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc
    18841 acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat
    18901 ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg
    18961 acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact
    19021 aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt
    19081 tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta
    19141 ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga
    19201 agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca
    19261 gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga
    19321 caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct
    19381 aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt
    19441 tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa
    19501 aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt
    19561 gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac
    19621 atccacgtag atgtaatgga tggccgattt gtcccaaata tcacattagg gccaaatatt
    19681 gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat
    19741 ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc
    19801 gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct
    19861 ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct
    19921 gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa
    19981 acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca
    20041 atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg
    20101 gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg acaaatcgaa
    20161 cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa
    20221 aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg
    20281 ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg
    20341 tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca
    20401 gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa
    20461 gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc
    20521 tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt
    20581 caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg
    20641 aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag
    20701 tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt
    20761 tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat
    20821 tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt
    20881 tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa
    20941 attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa
    21001 aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc
    21061 tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt
    21121 tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc
    21181 tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag
    21241 ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa
    21301 agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac
    21361 tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat
    21421 cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa
    21481 tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc
    21541 agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa
    21601 gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt
    21661 tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat
    21721 taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga
    21781 aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt
    21841 gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga
    21901 tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca
    21961 ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat
    22021 taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta
    22081 tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt
    22141 caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac
    22201 gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag
    22261 gtttttagga cctacactgc tgttgaacat gattatattt gtattattca tttttccctt
    22321 taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa agcgaaaaag
    22381 aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat cattttctga
    22441 aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac ataactatta
    22501 tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact taaatatatt
    22561 tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag tttaatatca
    22621 gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc tgccgcggcg
    22681 gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa
    22741 caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga aatcaacaca
    22801 caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc acaattagta
    22861 aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa
    22921 gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt attgaatgct
    22981 gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt aaaagcaaac
    23041 aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa
    23101 aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc tcaaaaaggc
    23161 gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc tgctgaacaa
    23221 gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc tgaagcagag
    23281 caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa
    23341 gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc tactcaagct
    23401 tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc ttcaatgact
    23461 gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac acctgaatct
    23521 tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac aacagcgcct
    23581 gaatcttcag caacagaaga atcaacaaca gtgcctgaat cttcagcaac agaagaatca
    23641 acaacagtgc ctgaatcttc aacaacagaa gaatcaacaa cacctgcgcc tacaacacca
    23701 tcaacagatc aaagtgttga tacaggaaac ggcacaggaa gtagtactcc ggctccaacg
    23761 ccaacaccaa cacctgaaca accaaaacct gtaacacctg ctccagctcc ttctggaagt
    23821 gtgaatggtg cagcaatcgt agcagaagct tacaaatata ttggtactcc atatgtttgg
    23881 ggcggtaaag atccaagtgg ctttgactgc tcagggttca cacgctatgt ttacatgcaa
    23941 gtaactggtc gtgacatcgg tggttggaca gttcctcaag aatcagctgg aacaaaaatt
    24001 tcagtttctc aagcaaaagc tggagacttg ttattctggg ggtcacaagg cggaacttac
    24061 cacgtagcga ttgctttagg cggaggacaa tatatccatg ctcctcaacc aggcgaaagc
    24121 gtaaaagttg gttcagttca atggtttgcc cctgactttg ctgtcagcat gtaagaatga
    24181 ttaatcaaaa agagttagcc cattctggac taactctttt tttattgtga taaaatagaa
    24241 atcagataaa ttcttctgtc aattccatga acgtttggat atcttcagtc accatgtcga
    24301 tacctgcttg ccaaaaatct ggttgggtaa gatcgacatt caaatgtttc ttcgcaagat
    24361 cttccgtcgt cattgaggca gtatcgcgta gtaaatggat atattcttct tcaaaatttg
    24421 ctcccttttg gttggcgtaa gcatagatgc ccaaactaaa gaggtagcca aatgtgtatg
    24481 ggaagttata aaacggtacg tcatcaataa agaaatgcaa ttttgcagcc cagaaatatg
    24541 gatggtaagt agctaaagct ccttgatagc cttctttttg cgcctcaacc atcatctctg
    24601 tgatcttatc ttctgagact aatccttctt gccgtgcttc atagaatcga ttttcaaaaa
    24661 tgaatcggga atggatattc atgaacatag ctaaagcatt ttgcagtttt gtatctagca
    24721 aattgatttt ttcttctttt gtttttgcga cttttaatgt agcatcggct acgatcagtt
    24781 ctgcgaatgt acttgctgtt tctgcgacat tcatggcata ttcgcgattt aaagaaggta
    24841 aatcccacat cgtgctgcta tggaaagcat gtcctagttc atgagcaagt gtcgcaacct
    24901 cattaacaga attactgtag gtcataaaga tccttgattc ttttgtttct ggtaactcag
    24961 tacaataacc accaggacgt ttcccaggac gatcttctgc ttcgatccag ctcttttcaa
    25021 aagcagattg ggcaaattta gccatttttg gactgaattt gttaaagttt tcgataataa
    25081 atgctgctgc ttcgtcaaaa ctaaaggttt tttctttcaa atcgcctaaa ataataggtg
    25141 catcttgatc ttgccattcc attttttctt taccgaaaag attagctttt ctagtcaagt
    25201 aattaacaag gggttgtttg tttttctgga ttgtatccca catgacagac aatgtttctt
    25261 tcttcaaccg attgtactct aatggttttt gtagaaaatc tgtgacacca tgaagttcat
    25321 agtcagaaag tctaaaacca tctaaatgat tcaaagtatc cgtgaagagg ggtgcctttt
    25381 ctttccaggc ttcttcccat gctgcgaaaa gtgtttctct gacttttgga tctggatcgc
    25441 ccatcatctt attgaaggct tgtccagcag ataattcgac tacttgtcca tcttgttcga
    25501 agggaatcga aatgctggct acaatcgtat cataatgact gctccaagca tttagaccat
    25561 ctaaagaaag cgtatttata atgttttctt cagcttctga taataattgt gagccatcac
    25621 gacgaatctc gtttaaacga aaagcaattg tttcaaacga agattgagaa agcatctgat
    25681 tccaatcatt gtcagatatt tctgcaaatt tctttgacaa tacagtttcg gctgattgca
    25741 gacgaggcag cattgcgtaa agcttgcctg ataagacctt tgcatctgaa tcattgacat
    25801 tggcagaaag caatgcagta atatagctgc tgcattgggt aaaaccattc gtaatcgttt
    25861 cttgcaattg aagaatagcg tttaactgct ctgcattgtc agaactaggt gaccattttg
    25921 ttacgcggtg ataatactcg ttcatttggt cttccaattg cttcatccgt tggttcaaag
    25981 catcagaatg actgcctccg gaaaaaatag aatctagatt ccaatttaat gaataactca
    26041 tgaaaaaact cccttcatat tgtaatacaa aattaagtat agcacttttt tatggaagaa
    26101 gtctctatga aaatgattgt tgtttcttca aaaaaagtct aagataaaat acagaggtga
    26161 cttatgaatt cttcaaaaaa acaaatgaaa aacggaaata tttatttagt aatcgcactg
    26221 atagtcgtta taatcttatt ttacagttct tctcaaactt acggacagca atcacaagtt
    26281 tcgcgattag aaagctggct gcctaatcag ccatttaaag acacattagc gggtatacgt
    26341 tttaattatg gtggaagtat ggtgagtatc gaccatctgg gctacgccaa atttttagag
    26401 ttctttttaa ggaagggcgc acatttcggt acctatttta ttttgggagg cagcttgttt
    26461 ttaggtgttt tccccaagct aaaaatttgg tggctgacag cgatccttgc ttggctttca
    26521 gcaacagggt acgcaggttt ggacgaattc catcagatga tgaccggtgg acgaacacct
    26581 atgttccaag atgtcatgtt ggattcgatc ggtgctctga ctgctgtgat attatgtgtg
    26641 ctttttgcgg cattcaagaa gcgtttatag attggctgtg gctttgtttt tttgtgaaat
    26701 agtactagaa tagaagtgta ttctcaaaag aacaagggag gaaaaatcat gtcaggacat
    26761 agtaaatggc ataatatcca aggaagaaaa aatgcccaag atgcaaaacg agggaaagtc
    26821 tttcagaaac tttctcgtga aatttatatg gcagctaaag caggcggtcc tgatccatct
    26881 atgaatccag ctttgcggct tgttgtggac aaagcaaaag ctgcaaatat gcctaacgac
    26941 aatgtggaac gggcgattaa aaaggctacg agtgccgtag atgaaacgaa ttacgatgaa
    27001 atcacgtatg aaggatatgg tccgggtgga gtaggaatct tagtccatgc attgacagat
    27061 aaccgtaatc ggacagcgac aaatgtccga gtagctttta ctcgaaacgg tggatcactg
    27121 ggagaaacgg gttcagtcag ttacttgttt gaccgcaaag gatatatcgc tattgaacgc
    27181 gatgggttga ctgttgatga agagacgatg tttgaggatg tattagaggc gggggcagaa
    27241 gatctgcaaa cttctcctga agtattcgag atttatactg caccagaaga ttttgctgct
    27301 gtacgagatg agttggaaaa agagttcact ttggcgcaag cagaattgac gatgattcca
    27361 caaacgacga ttgatttaaa tgaggaacaa aaagagcaat tggaacgtct agtagataag
    27421 ctagaagacg atgatgatgt ttcagaagtc tttactgctg ctgaaatgta agtagcacat
    27481 tgttagataa taaaaagaaa gcacctgtac caatcgtacg ggtgctttct ttttatatat
    27541 tatttttgtt agaagaccaa tacaggcgtt cctttttcga ccatcccgaa taattccttc
    27601 attacacttg gtggggtgtt gatgcagccg tgtgaaccgc gggttttcca taagtctcca
    27661 ccgtattcag gttgccagtc tgaatcgtgg ataccaacac ctgtccagtc aatcggcatc
    27721 cagtagttta ccggactctc gtaaggtgtt ccatcgtcat ttgttccttt taaagttgca
    27781 tcttcttctt tattccaaac atagaagact ccagcaggtg taggtgtagt tggctttccg
    27841 gaaacgatat ctgtttcaag ggccactttt ccgtctttgt aataccacat atgctggttc
    27901 tccagatcaa cttcgatata cgtatcttcg atcaatggat gatcagcggt tgtgccacct
    27961 tgcacaatgg gtgaacgagt aaaatcttgg cctgccagaa tcgctttttt caatgcttct
    28021 gtctcactgt ctgtttggat cgtccaactg taagtgccaa ctgggaccgt aacttctcct
    28081 ctttttgtac ttttaaattt tgtgtcattc gtacttgtgt tatactttgt gcctaaatct
    28141 gttacatatt gacgtacttg ttctgtatct aaattgactt ttccatcatt gtaggtcagc
    28201 caactcataa tatcacttga tgggatctgg aaagtctcac cgtttatact gtaagtagct
    28261 tttacatttg cgatagcatt catggatgcg agttgttctt tcagtgagct gtcttcagat
    28321 gttacttttg gtttttcttt gaattcagtc aattcaattg tatctttacc agagtttacc
    28381 gcacttttta attgtttgac cgcagcatcg acgtcgatcg tatttccatt gacttcaggc
    28441 ttgataacga aagagtcacc tgctttttcg atcgttgcat cagtagtagg tgtccggtct
    28501 ttgttcaaat cagtcagctt agttgtcaat gtctgaacgg tagtgtctag tttttggtgg
    28561 ttttgactga caggatctat ttcttgtttt tctgccgctg agacataagt cattccccaa
    28621 gtccatccgt tttgttcatt taggatcgga gaaagctccg aagtgaaatc atctttgtag
    28681 ccgagctcgg attttggtac ttcctgccag acttttccat cttctttaat agccagtttt
    28741 ttatcagaat aagcttcttt taatttttca tttgcttttt cgactgttag attgctgaca
    28801 tttattccat ttatttctgt attcggcaaa aaatgctttg tgtagtatgt actgcgatag
    28861 gtataaaagc tgatcagcac taagagaaca ccaagaactg aaagcaaagc aacatgtttc
    28921 gatttttttc gatgcgaaga tcttgtcata tcgacaactc cttaagtaaa agtttagggt
    28981 ggaacggtct catgatagca taatttagag gaaaagacga aggttatctc tactttatga
    29041 aaaaatttag aaaccataag ggtgataaaa taaaaaaacg gaaaacaccg aatagatgtt
    29101 ttccgtcatg ttcaaagatt attcagcgtc tagtttagca aaagcttcat ctagaatttc
    29161 ttttaattgc ttagcagaag cagccatacg gtcttgctcg ctgtcactta aaggaatttc
    29221 gattactttt tggatacctt gacggttgat aacagcaggt gcaccaatgt agatgtcgtt
    29281 caatccatat tcgccattca tgaatactga taaaggaaga acagcatttt catcatctaa
    29341 gatggcacga gtgatacgtg ctaatgctac tgcgatacca tagaaagtag cgcctttttt
    29401 ctcgataatt gtgtaagcag cgtcacgtac gccaaagaat aggttaacca tagcttcttc
    29461 atcgatttct gggttgtttt ttacccattc atagatttgc aagccggcaa catttgcatg
    29521 tgaccaaact gggaattctg aatctccgtg ttcacctaag atgtaagcat ggacgttacg
    29581 tgcatcaacg tcaactaatt ccgcaagtgc ttgacggaaa cgagctgagt caagagaggt
    29641 acctgaaccg attacgcgtt ctttagggaa acctgagaat ttccaagttg agtaagtcaa
    29701 aatatctact gggttagcgg caactaaaaa gataccgtta aatcctgatt caacgatttg
    29761 tgtgacaaca tcgcggttga tttttaggtt tttgtttact aagtctaaac gagtttcacc
    29821 tggtttttgt ggtgcgccag ctgtaataac aactaagtca gcatcatgtg cgtctgcgta
    29881 agtagcagaa tagatttttt taggtgaagt aaatgccaaa gcgtgagaca agtcaattgc
    29941 gtctccttca gttttagcag tattgatatc cacaatacct acttcttgag cgatattttg
    30001 agtaactaga gcaaaggcat agctagaacc tacggcgccg tctccaacaa gaataacttt
    30061 ttgatgatct tttttttctg cagctgcagt catttagtat caatccttcc attgatttgt
    30121 tttttcccat gcttatgcta acatttctca agccccatgt cacgctattt ggctataaaa
    30181 tataacagat aacaaggttg tattatatgt aaaagcttac atgt
//
LOCUS       DAJSNV010000023        29956 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSNV010000023 DAJSNV010000000
VERSION     DAJSNV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.024"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(61..2424)
                     /locus_tag="ON516_001051"
     CDS             complement(61..2424)
                     /locus_tag="ON516_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCR3344545.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="ON516_001052"
     CDS             2872..3093
                     /locus_tag="ON516_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344546.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ON516_001053"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ON516_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3344547.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ON516_001054"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ON516_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3344548.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ON516_001055"
     CDS             complement(5894..6205)
                     /locus_tag="ON516_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3344549.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ON516_001056"
     CDS             complement(6202..6495)
                     /locus_tag="ON516_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3344550.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ON516_001057"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ON516_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3344551.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ON516_001058"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ON516_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3344552.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ON516_001059"
     CDS             complement(8333..12685)
                     /locus_tag="ON516_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3344553.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ON516_001060"
     CDS             complement(12897..14606)
                     /locus_tag="ON516_001060"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3344554.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ON516_001061"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ON516_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3344555.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ON516_001062"
     CDS             complement(16103..16903)
                     /locus_tag="ON516_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3344556.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ON516_001063"
     CDS             complement(16900..17712)
                     /locus_tag="ON516_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3344557.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ON516_001064"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ON516_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3344558.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="ON516_001065"
     CDS             complement(18467..19189)
                     /locus_tag="ON516_001065"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3344559.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="ON516_001066"
     CDS             complement(19325..20206)
                     /locus_tag="ON516_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3344560.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ON516_001067"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ON516_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3344561.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ON516_001068"
     CDS             21446..21925
                     /locus_tag="ON516_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3344562.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ON516_001069"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ON516_001069"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3344563.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="ON516_001070"
     CDS             23226..24011
                     /locus_tag="ON516_001070"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3344564.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="ON516_001071"
     CDS             complement(24357..26048)
                     /locus_tag="ON516_001071"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3344565.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ON516_001072"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ON516_001072"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3344566.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ON516_001073"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ON516_001073"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3344567.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ON516_001074"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ON516_001074"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3344568.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7988 a         6446 c         5341 g        10181 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatatgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatg
//
LOCUS       DAJSNV010000024        29354 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSNV010000024 DAJSNV010000000
VERSION     DAJSNV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.025"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..138)
                     /locus_tag="ON516_001075"
     CDS             complement(<1..138)
                     /locus_tag="ON516_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3344569.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            complement(232..1281)
                     /locus_tag="ON516_001076"
     CDS             complement(232..1281)
                     /locus_tag="ON516_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3344570.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1363..2052)
                     /locus_tag="ON516_001077"
     CDS             complement(1363..2052)
                     /locus_tag="ON516_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3344571.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2158..2526)
                     /gene="rplL"
                     /locus_tag="ON516_001078"
     CDS             complement(2158..2526)
                     /gene="rplL"
                     /locus_tag="ON516_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3344572.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2579..3082)
                     /locus_tag="ON516_001079"
     CDS             complement(2579..3082)
                     /locus_tag="ON516_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3344573.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3286..3413)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3492..4181)
                     /gene="rplA"
                     /locus_tag="ON516_001080"
     CDS             complement(3492..4181)
                     /gene="rplA"
                     /locus_tag="ON516_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3344574.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4285..4707)
                     /gene="rplK"
                     /locus_tag="ON516_001081"
     CDS             complement(4285..4707)
                     /gene="rplK"
                     /locus_tag="ON516_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3344575.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5050..5718
                     /gene="sdaAB"
                     /locus_tag="ON516_001082"
     CDS             5050..5718
                     /gene="sdaAB"
                     /locus_tag="ON516_001082"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3344576.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5731..6603
                     /gene="sdaAA"
                     /locus_tag="ON516_001083"
     CDS             5731..6603
                     /gene="sdaAA"
                     /locus_tag="ON516_001083"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3344577.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6694..7713)
                     /locus_tag="ON516_001084"
     CDS             complement(6694..7713)
                     /locus_tag="ON516_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3344578.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7886..8791)
                     /locus_tag="ON516_001085"
     CDS             complement(7886..8791)
                     /locus_tag="ON516_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3344579.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8850..9395)
                     /gene="nusG"
                     /locus_tag="ON516_001086"
     CDS             complement(8850..9395)
                     /gene="nusG"
                     /locus_tag="ON516_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3344580.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9496..9666)
                     /gene="secE"
                     /locus_tag="ON516_001087"
     CDS             complement(9496..9666)
                     /gene="secE"
                     /locus_tag="ON516_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3344581.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9690..9842)
                     /gene="rpmG"
                     /locus_tag="ON516_001088"
     CDS             complement(9690..9842)
                     /gene="rpmG"
                     /locus_tag="ON516_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3344582.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            10004..10648
                     /locus_tag="ON516_001089"
     CDS             10004..10648
                     /locus_tag="ON516_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3344583.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10704..11207)
                     /locus_tag="ON516_001090"
     CDS             complement(10704..11207)
                     /locus_tag="ON516_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3344584.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11220..11266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11804..12769
                     /locus_tag="ON516_001091"
     CDS             11804..12769
                     /locus_tag="ON516_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3344585.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12868..14040)
                     /locus_tag="ON516_001092"
     CDS             complement(12868..14040)
                     /locus_tag="ON516_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344586.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14027..15172)
                     /locus_tag="ON516_001093"
     CDS             complement(14027..15172)
                     /locus_tag="ON516_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344587.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15186..16118)
                     /locus_tag="ON516_001094"
     CDS             complement(15186..16118)
                     /locus_tag="ON516_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344588.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16235..17314
                     /locus_tag="ON516_001095"
     CDS             16235..17314
                     /locus_tag="ON516_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3344589.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17311..17940
                     /locus_tag="ON516_001096"
     CDS             17311..17940
                     /locus_tag="ON516_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3344590.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(18050..18277)
                     /locus_tag="ON516_001097"
     CDS             complement(18050..18277)
                     /locus_tag="ON516_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3344591.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18380..20968)
                     /locus_tag="ON516_001098"
     CDS             complement(18380..20968)
                     /locus_tag="ON516_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3344592.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20988..21647)
                     /locus_tag="ON516_001099"
     CDS             complement(20988..21647)
                     /locus_tag="ON516_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3344593.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21696..22199)
                     /gene="trmL"
                     /locus_tag="ON516_001100"
     CDS             complement(21696..22199)
                     /gene="trmL"
                     /locus_tag="ON516_001100"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3344594.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22420..23286)
                     /locus_tag="ON516_001101"
     CDS             complement(22420..23286)
                     /locus_tag="ON516_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3344595.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23395..24036
                     /locus_tag="ON516_001102"
     CDS             23395..24036
                     /locus_tag="ON516_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3344596.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24098..25459)
                     /gene="mgtE"
                     /locus_tag="ON516_001103"
     CDS             complement(24098..25459)
                     /gene="mgtE"
                     /locus_tag="ON516_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3344597.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25477..26376)
                     /locus_tag="ON516_001104"
     CDS             complement(25477..26376)
                     /locus_tag="ON516_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3344598.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26378..27175)
                     /locus_tag="ON516_001105"
     CDS             complement(26378..27175)
                     /locus_tag="ON516_001105"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3344599.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27153..27857)
                     /locus_tag="ON516_001106"
     CDS             complement(27153..27857)
                     /locus_tag="ON516_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3344600.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27988..28563
                     /locus_tag="ON516_001107"
     CDS             27988..28563
                     /locus_tag="ON516_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3344601.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28681..>29354
                     /locus_tag="ON516_001108"
     CDS             28681..>29354
                     /locus_tag="ON516_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3344602.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8631 a         6206 c         5274 g         9243 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc
      181 gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      241 accacgggta aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg
      301 tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag
      361 caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac
      421 aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg
      481 tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca
      541 attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc
      601 aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg
      661 cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg
      721 cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata
      781 gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat
      841 ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc
      901 tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa
      961 agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc
     1021 agataacgct accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac
     1081 taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc
     1141 acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat
     1201 attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg
     1261 tgttttttca ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg
     1321 aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt
     1381 tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac
     1441 acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca
     1501 cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag
     1561 aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat
     1621 atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg
     1681 attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat
     1741 gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa
     1801 accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt
     1861 caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc
     1921 tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa
     1981 ccgatagccc actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat
     2041 tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata
     2101 aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta
     2161 tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc
     2221 tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt
     2281 caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc
     2341 tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc
     2401 agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag
     2461 gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa
     2521 tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt
     2581 aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga
     2641 ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg
     2701 ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt
     2761 ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt
     2821 cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag
     2881 cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat
     2941 cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga
     3001 ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt
     3061 ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt
     3121 tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca
     3181 cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct
     3241 ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa
     3301 actctatgcc accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta
     3361 ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact
     3421 agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt
     3481 ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg
     3541 atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca
     3601 tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt
     3661 ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg
     3721 tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct
     3781 tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca
     3841 actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg
     3901 aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca
     3961 gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct
     4021 tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg
     4081 tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact
     4141 ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg
     4201 tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga
     4261 agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc
     4321 ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc
     4381 agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc
     4441 tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt
     4501 aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc
     4561 ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg
     4621 acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt
     4681 aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta
     4741 attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata
     4801 atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt
     4861 tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa
     4921 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
     4981 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
     5041 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
     5101 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
     5161 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
     5221 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
     5281 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
     5341 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
     5401 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
     5461 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
     5521 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
     5581 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
     5641 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
     5701 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
     5761 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
     5821 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
     5881 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
     5941 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
     6001 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
     6061 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
     6121 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
     6181 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
     6241 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
     6301 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
     6361 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
     6421 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
     6481 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
     6541 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
     6601 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
     6661 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
     6721 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
     6781 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
     6841 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
     6901 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
     6961 tacttcaaag gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat
     7021 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
     7081 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
     7141 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
     7201 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
     7261 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
     7321 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
     7381 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
     7441 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
     7501 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
     7561 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
     7621 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
     7681 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
     7741 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
     7801 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
     7861 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
     7921 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
     7981 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
     8041 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
     8101 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
     8161 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
     8221 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
     8281 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
     8341 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
     8401 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
     8461 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
     8521 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
     8581 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
     8641 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
     8701 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
     8761 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
     8821 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
     8881 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
     8941 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
     9001 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
     9061 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
     9121 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
     9181 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
     9241 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
     9301 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
     9361 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
     9421 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
     9481 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
     9541 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
     9601 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
     9661 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
     9721 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
     9781 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc
     9841 atttacttct gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact
     9901 ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat
     9961 taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact
    10021 tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt
    10081 aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat
    10141 tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat
    10201 gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc
    10261 tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga
    10321 aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg
    10381 gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt
    10441 ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga
    10501 tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc
    10561 gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat
    10621 tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg
    10681 ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa
    10741 taatacatca atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca
    10801 taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg
    10861 ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt
    10921 tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc
    10981 atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc
    11041 aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc
    11101 cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca
    11161 tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt
    11221 ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat
    11281 aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag
    11341 cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg
    11401 gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga
    11461 aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct
    11521 agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt
    11581 aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt
    11641 tcctagactt ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac
    11701 aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag
    11761 gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca
    11821 tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt
    11881 accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca
    11941 cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga
    12001 acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca
    12061 tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt atattccagc
    12121 aggttctgga aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact
    12181 tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa
    12241 aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat
    12301 cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc
    12361 attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct
    12421 ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa
    12481 gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt
    12541 cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt
    12601 acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt
    12661 acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg
    12721 accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt
    12781 ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt
    12841 tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata
    12901 gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa
    12961 gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact
    13021 gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac
    13081 catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat
    13141 aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc
    13201 cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt
    13261 tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa
    13321 cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat
    13381 gggcgcactc tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct
    13441 gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt
    13501 caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga
    13561 gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag
    13621 aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc
    13681 tgggcgactc tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc
    13741 tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt
    13801 ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc
    13861 atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt
    13921 attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc
    13981 tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat
    14041 aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca
    14101 accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac
    14161 tttcgctgat caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc
    14221 aatacttcac ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg
    14281 aaatcaagat gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg
    14341 tacctaacaa aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac
    14401 gttcaccaaa gtctacttga tagataaaac gaaagacagc taacataata taaatctctg
    14461 tcaaaacaat cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag
    14521 gaatcaagga caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc
    14581 ggacccccca catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac
    14641 ttttgaaact catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa
    14701 cagtcatgat gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac
    14761 caatacgctg taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt
    14821 gacccacatg gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg
    14881 catttagctt ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat
    14941 cattgacttc gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg
    15001 ctttgattac aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa
    15061 aggccgccat aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg
    15121 atttatttcg cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc
    15181 ctcctctagt ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct
    15241 tctttggttg ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc
    15301 aacaatactg tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt
    15361 ccatcagaag ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg
    15421 tcgatcgtca aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta
    15481 gcaatcacct gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc
    15541 atgtaatgtg ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct
    15601 aaaattttat tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac
    15661 cgcggcttgt gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt
    15721 tctcctggac gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc
    15781 aaggctttcc ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct
    15841 tgcacactta gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg
    15901 acagcataat tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa
    15961 tttaacaaag aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac
    16021 agacctaaga cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct
    16081 ccatagcgtt tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt
    16141 tcttgttgat ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc
    16201 acacatgaca tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga
    16261 aactagcttt ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat
    16321 gcaagttatc tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac
    16381 aaagccaaat tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct
    16441 tgtcctgttt ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta
    16501 cttgtttttg ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt
    16561 cgcctttgtc gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac
    16621 gaccgatctt gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga
    16681 gttatcggaa caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat
    16741 tgtggaagaa cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc
    16801 aagtgcgatt ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga
    16861 accattagaa aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt
    16921 ccatgacctc catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga
    16981 ttttccttct tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca
    17041 aaactgcttc ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc
    17101 caaaaccgtt gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa
    17161 tgatggcacc agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca
    17221 acgtgttcaa gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt
    17281 aacgatcatt ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat
    17341 ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta
    17401 ggcattggtc attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt
    17461 ttgctgacgg acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg
    17521 gctaaatttc ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc
    17581 cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt
    17641 attccttctg tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag
    17701 actttcactc aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt
    17761 agtgaacgtg agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt
    17821 gcagatgcct tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa
    17881 aagctagatt tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa
    17941 gtcttatcta acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca
    18001 catcttctat tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg
    18061 cagggctgcc aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt
    18121 ctaacgtttt ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat
    18181 tcaatttgaa ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg
    18241 ctaatttcgc taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt
    18301 tcagcttagc atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag
    18361 ctatttctca tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt
    18421 tttccatacc aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga
    18481 aatctttaga aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa
    18541 acagatctgg ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat
    18601 cttctacagg tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa
    18661 atggatcatt tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat
    18721 tcattttatc attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat
    18781 tcgctacaca agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc
    18841 gagtcactgc cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg
    18901 gcaaaataac catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa
    18961 gagtgatttt attccattcg tttcttttat aagtgacctc atttgcatca aactggataa
    19021 ctaattcatc cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca
    19081 tatctccatt gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta
    19141 tccgataaac ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata
    19201 tctcctgcat cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta
    19261 atacttggat atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga
    19321 tcaagggttc aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg
    19381 tgaaatcttt tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac
    19441 catcaccttg ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa
    19501 gatcatggag tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga
    19561 taacttgcat attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca
    19621 tagacatctc atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg
    19681 ttttttcccg tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg
    19741 tttcgttcat tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt
    19801 cttgggtata ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga
    19861 ttccgttgat cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg
    19921 aacggattgc ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt
    19981 tttcgatacg ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac
    20041 gactgagaag gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt
    20101 cgtaaagatt agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag
    20161 tagcaacctg atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt
    20221 gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa
    20281 tctgatgcaa gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt
    20341 cggctagatt atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg
    20401 gattttcttc gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta
    20461 actgactacc gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta
    20521 gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta
    20581 ataccgaagg atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt
    20641 tttctgctgt tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc
    20701 catttgcagt tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt
    20761 taggatgatc cactaattct ccataaaaac gataagtctc attttcttga atatcgccga
    20821 aacttccggt gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta
    20881 ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa
    20941 cgaaataagg catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt
    21001 tctggtgctg tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc
    21061 aataatctgc aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc
    21121 atttcacgca actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa
    21181 gctccatgtc ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca
    21241 acagtttcga tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca
    21301 tacagatcta aatgattacg caaattcggc agattatccg gatatttctg gtacatttca
    21361 tcgatcgact gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa
    21421 atctctaccg gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt
    21481 gctgtactgg aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg
    21541 atttcttcgt aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt
    21601 tcctgattcc attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc
    21661 ctgtctttct tttattttac tctataataa actgtctaat ccagcttgtc attttcatag
    21721 tgatgcgtca attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt
    21781 gcagctgtat tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa
    21841 cacttttctt cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat
    21901 aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat
    21961 tttgagatca aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg
    22021 tggtaagtaa tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca
    22081 tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta
    22141 cgggcaatat ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc
    22201 aggatcattc ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata
    22261 aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc
    22321 tcactaactt ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct
    22381 aatataacac cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg
    22441 ttttctctta gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt
    22501 atataagtct gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag
    22561 atctttttga ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa
    22621 tttcattgct tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg
    22681 ttgattcgga taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt
    22741 caacacttct ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa
    22801 gatatttaaa accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac
    22861 acaccaaaat gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc
    22921 aaatccaaca gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc
    22981 tccgcagcca acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat
    23041 aacgggctga tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc
    23101 gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa
    23161 acggtgatta tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac
    23221 cggacagcgg aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt
    23281 taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg
    23341 ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc
    23401 aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat
    23461 cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc
    23521 gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct
    23581 agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt
    23641 gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg
    23701 ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc
    23761 catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta
    23821 gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa
    23881 aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct
    23941 ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa
    24001 gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc
    24061 aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata
    24121 aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg
    24181 gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac
    24241 ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga
    24301 taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg
    24361 tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat
    24421 cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag
    24481 ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat
    24541 agtgactgat taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca
    24601 aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa
    24661 gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga
    24721 cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc
    24781 taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc
    24841 tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa
    24901 cccctactag ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat
    24961 ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa
    25021 caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga
    25081 tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca
    25141 gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat
    25201 agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat
    25261 cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt
    25321 ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc
    25381 tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta
    25441 attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac
    25501 tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg
    25561 gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc
    25621 gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg
    25681 atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc
    25741 tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga
    25801 agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct
    25861 tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa
    25921 ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag
    25981 tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac
    26041 acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg
    26101 tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa
    26161 aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc
    26221 tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt
    26281 tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg
    26341 agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat
    26401 ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg
    26461 ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta
    26521 caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga
    26581 tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa
    26641 aggcattgat actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac
    26701 cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga
    26761 tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta
    26821 aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac
    26881 taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc
    26941 gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca
    27001 gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga
    27061 ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac
    27121 gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct
    27181 gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt
    27241 cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc
    27301 tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct
    27361 ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc
    27421 gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca
    27481 ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa
    27541 tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg
    27601 atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct
    27661 aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg
    27721 actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg
    27781 atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct
    27841 tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc
    27901 atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg
    27961 caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct
    28021 atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac agataaagat
    28081 ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat
    28141 tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct
    28201 gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc
    28261 ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc
    28321 gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt
    28381 gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg
    28441 gaactagagg taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt
    28501 ttttctttac cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat
    28561 tagaaacctt tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt
    28621 tataggatgt gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat
    28681 atgatcgaaa tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa
    28741 cagttattac aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt
    28801 cgctttcttc ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt
    28861 tctcagaaca atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg
    28921 gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg
    28981 caagaagcag tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt
    29041 gaagaaatcg gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa
    29101 gaagtattct tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct
    29161 gcagtcgttt acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt
    29221 acaagtagcc tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt
    29281 tttaatggtg aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta
    29341 cacttgcttt ctaa
//
LOCUS       DAJSNV010000025        28411 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSNV010000025 DAJSNV010000000
VERSION     DAJSNV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.026"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            48..1688
                     /locus_tag="ON516_001109"
     CDS             48..1688
                     /locus_tag="ON516_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3344603.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1842..3164
                     /locus_tag="ON516_001110"
     CDS             1842..3164
                     /locus_tag="ON516_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3344604.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3226..3657)
                     /locus_tag="ON516_001111"
     CDS             complement(3226..3657)
                     /locus_tag="ON516_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344605.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3721..4557)
                     /locus_tag="ON516_001112"
     CDS             complement(3721..4557)
                     /locus_tag="ON516_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3344606.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4747..5049)
                     /locus_tag="ON516_001113"
     CDS             complement(4747..5049)
                     /locus_tag="ON516_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3344607.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5075..5506)
                     /gene="ruvX"
                     /locus_tag="ON516_001114"
     CDS             complement(5075..5506)
                     /gene="ruvX"
                     /locus_tag="ON516_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3344608.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5503..5769)
                     /locus_tag="ON516_001115"
     CDS             complement(5503..5769)
                     /locus_tag="ON516_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3344609.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDSAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6023..6748)
                     /locus_tag="ON516_001116"
     CDS             complement(6023..6748)
                     /locus_tag="ON516_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3344610.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7114..7184)
                     /locus_tag="ON516_001117"
     tRNA            complement(7114..7184)
                     /locus_tag="ON516_001117"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7150..7152),aa:Gly,seq:ccc)
     gene            7301..8053
                     /locus_tag="ON516_001118"
     CDS             7301..8053
                     /locus_tag="ON516_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3344611.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8069..8548
                     /locus_tag="ON516_001119"
     CDS             8069..8548
                     /locus_tag="ON516_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3344612.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8799..10379)
                     /locus_tag="ON516_001120"
     CDS             complement(8799..10379)
                     /locus_tag="ON516_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3344613.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10372..11115)
                     /locus_tag="ON516_001121"
     CDS             complement(10372..11115)
                     /locus_tag="ON516_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344614.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11291..12067)
                     /locus_tag="ON516_001122"
     CDS             complement(11291..12067)
                     /locus_tag="ON516_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3344615.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12315..13151)
                     /locus_tag="ON516_001123"
     CDS             complement(12315..13151)
                     /locus_tag="ON516_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3344616.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13162..14091)
                     /locus_tag="ON516_001124"
     CDS             complement(13162..14091)
                     /locus_tag="ON516_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3344617.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14283..14636
                     /locus_tag="ON516_001125"
     CDS             14283..14636
                     /locus_tag="ON516_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3344618.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14710..15366)
                     /locus_tag="ON516_001126"
     CDS             complement(14710..15366)
                     /locus_tag="ON516_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3344619.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15592..17526
                     /locus_tag="ON516_001127"
     CDS             15592..17526
                     /locus_tag="ON516_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3344620.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17612..18835)
                     /locus_tag="ON516_001128"
     CDS             complement(17612..18835)
                     /locus_tag="ON516_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3344621.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(18829..20727)
                     /locus_tag="ON516_001129"
     CDS             complement(18829..20727)
                     /locus_tag="ON516_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3344622.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20721..21839)
                     /locus_tag="ON516_001130"
     CDS             complement(20721..21839)
                     /locus_tag="ON516_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3344623.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(21922..22755)
                     /locus_tag="ON516_001131"
     CDS             complement(21922..22755)
                     /locus_tag="ON516_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3344624.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22748..23665)
                     /locus_tag="ON516_001132"
     CDS             complement(22748..23665)
                     /locus_tag="ON516_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344625.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23892..25016)
                     /locus_tag="ON516_001133"
     CDS             complement(23892..25016)
                     /locus_tag="ON516_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3344626.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25199..26107
                     /locus_tag="ON516_001134"
     CDS             25199..26107
                     /locus_tag="ON516_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344627.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26248..27861
                     /locus_tag="ON516_001135"
     CDS             26248..27861
                     /locus_tag="ON516_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3344628.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8831 a         5588 c         4851 g         9141 t
ORIGIN      
        1 tctgactcct atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggc
       61 taaacttatt aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg
      121 caaaatacgg aatcactggt atgcttccaa gtactgtcca aacattggaa caacaatctg
      181 tccaagctta cggtcaatac ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa
      241 tgaatctatt caatacgaac cggacattgt tttataaatt gatgggcgaa catttagttg
      301 aattcatgcc tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa
      361 ttttcttgaa accacaagat gctgtatttc tttcaatcga cgaaccagaa aatatcaaag
      421 caagtttgaa aaacgcagca gatggacgcg acattcgttt gatcgttgtg acagatgcag
      481 aaggaatctt aggtatgggt gactggggtg tgaacggtgt tgacatcgct atcggtaaat
      541 tgatggtcta cactgccgcg gctggtatca atcctgcaca agtcttgcct gtatcaatcg
      601 acgctggtac taataacgaa gaattattga ataatccatt atacttaggg aatcgtcatg
      661 ctcgtgtaga aggcgaaact tactacgaat tcattgatca attcgttcaa tcagctactg
      721 aattattccc agaattgtta ttacattggg aagattttgg acgaggaaat gctgcaacga
      781 tcttagaaaa atacgaagat aaaatcacta ctttcaatga tgatatccaa ggcacaggaa
      841 tcgttgtact agctggtgta ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc
      901 aaacgatctt gacatttggt gctggtactg ctggtgtagg tatcgcaaac atccttttag
      961 atgaaatgat ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag
     1021 ataaacaggg tttattgttt gaagatacaa aaggattgac tcctggtcaa atcccattcg
     1081 ctcgtaaacg ctcagaattc actaatgctg atgaattgac aaacttagaa gcagttgtaa
     1141 aagcaatcca tccaacgatc atgatcggta cttctaccca acctggagca ttcactgaag
     1201 aaatcgtgaa agaaatggcg gctcataccc cacgtccaat catcatgcct ttgtctaatc
     1261 caacaaaatt ggcagaagca aaagcaaaag atttgattga atggacggac ggaaaagctt
     1321 tagttggaac aggtatccct gctgctgatg tggaatataa cggtgtaact taccaaatcg
     1381 gtcaagcaaa taatgcgttg atgtacccag gtttagggct tggtttgatt gcttcaacag
     1441 ctacacgagt aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg
     1501 atgtaacaaa accaggagca gctatcttgc ctccagtagc aaaaatcact gaattctctc
     1561 aaacgattgc tgaaactgtc gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa
     1621 tcacggatat caaagaagct gttgaatctg ctaaatgggt tcctgaatat aaatctttag
     1681 aagattaaga ttgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa
     1741 aaaagcgact gcggcaaaac agcagccagc caactactgc tcttttgccg cttctctttg
     1801 gcttttttca tacgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa
     1861 ccgcaactgc agttgaggaa aagaaaaaag aagggttttg gtcaacaaaa attaccggcg
     1921 taagtttgcc cctttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggaa
     1981 aattaccgac gaatatgatt ggcccaatcg ctgttttggt cattttcggt aatttatttc
     2041 attttatcgg aacaaaaatc cctatagtca aaagttattt aggtggtggc tccgtttttg
     2101 cgatctttgc ctctgccgcg attgctacat ttgggatctt acctgagtat gtagtaaaat
     2161 ccacagaaaa ctttgtcagc aatatgggct ttttagactt ttatattgct gccttgatta
     2221 ctggtagcat ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc
     2281 ccgttgcact tatttcaatg gtcgtctcct tttttgccgt aggactcgta ggtatgctta
     2341 tcggaaatgg ttttgcaaat tccgttttat atatctcatt gcccgcaatg gctggtggtg
     2401 ttggagccgg agctgtacct ttatcaacga tttatgcaaa tgttttaggt acggacgctt
     2461 cttctatcat ttcccgcctg attcctgcga cagcaatgac aaatgtccta gctatcattg
     2521 gtgcagcgtt agttgctcgt gcgggacaat caatgccaaa attcaacggt aatggtaaat
     2581 taatggaaaa aggcgacctt ggcgatggaa aacctagaaa tgtaaaacct tccattccac
     2641 aattaggtgt cggtttgatg gtatcattga ctttcttcat cttaggacaa atctgtaact
     2701 attttgttcc taaagtccat gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa
     2761 tcgcaaacct attaccagaa tattacgaag atgcagctat catgttcaac aacttgattg
     2821 tcaaaaactt gacagcagct gttcttgccg gtatcggtat tgcgttattg aatctaaatg
     2881 tattggcttc tgctttgaca tggcaatttg ttgtcttatg tttgacaagt gttatcgcga
     2941 tttcagtagt ttcaggattt gtcggtcgtt tgtttggttt atatccaatc gaatcaacta
     3001 ttactgctgg cttatgcaac aactcgatgg gtggtacagg aaatgtagct gtcttatctg
     3061 cagctaatcg gatggaactt attgcttttg ctcaaatggg caatcggctt ggtggtgcca
     3121 tcgttttgat catctcaggt ttcttgatgc aattactttc ttagtcaaaa aaaatacctg
     3181 atcatcaagt atcggcttga tgaccaggta ttttttgata aaaatttaca ggattttcaa
     3241 aaatcttttt tgcattttat cggaatgata accgatgcgt tcataaaatc ggtgtgcttc
     3301 tactctgtat tctgcagaat taagtcttat tgctgatatt cctcgtcttt tcgcttcttc
     3361 tacgccagtc atcaaggcct ttccgattcc tcttttttca gcatgtttag acacagcaag
     3421 tgccataata ttgaacatcg gatcgctgaa aatcgattca taggactccg cttgtacata
     3481 gccagctgct tttttagaag taggatcttc atagaccaag aaaaaatttt tttctggctc
     3541 cttcaacagt ttcttcattt gtctcaccgt aacggttaat ggtacatcat atcctaattg
     3601 ttccgcattg atttctcgta gtgcttctgc atctcctaaa cgcatttccc gcaacatcca
     3661 aatcaccagc caatttttat ttttttaaat gaattttttt atcacagtat actccttttt
     3721 tcacttaaaa aaagactttt cagtgatttt gacttgcttg aatccagatc cttctaatgt
     3781 ttgcaagatg acgaaagcat tgtaaagatt gatcaaaaga accacgatga caatcgcttg
     3841 aaaaataaag gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc
     3901 tccaataaca gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct
     3961 acttgttaaa gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga
     4021 atatagtgtt gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac
     4081 tggtccacca ccaataccta aaaaactagc taaaaagccc agaacccccc cacatataag
     4141 gtaccaagtc catgacgtca aatgaaattt tggctgatga tgcttggtgt agaagaacgc
     4201 aaaaaccaag gtcaataccg tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc
     4261 aaataactgt aaaaaaactt cgaatacaat gttgccgact actcctccaa caacagctcc
     4321 actagatacc caaccgacaa tctgccaatt cagtgatttc ccgcttgata cttgtcgcac
     4381 agtcgaaaca atggacatcg tgaataccgc aactgttgag taaaaagaaa tccctgcaac
     4441 agaatgggcg ccgataagat caagaatcgg tttgatcaac acgcctccac ccatcccgga
     4501 aatagcgcca actgtgttcg ccagaacgat caccacaaaa taaatcagac caatcatctt
     4561 gtatttcctt tctcttgttt atatctttta gcgattgtta ctcttttctt ttattataca
     4621 gtaaaagaaa caaacgaggc ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa
     4681 acgaaaaaac cgggctctac ttttcagaga gtccagcttt tcttacacct tatcgattct
     4741 tttgttctat tcttcttccg ccatgaatgt attgaatact tcttcgatca tatcccattc
     4801 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
     4861 ataagcttgt aattctacat cttcatcttc tgaaacacca gctggatata aaagaacata
     4921 gttacgtcca aattcttctt gtccgtcaac tgttagcaag atttcgtata atgtttcatt
     4981 tccttggtca tccaccaatg tgatgtgttc gtgtccttcg tgttcgtggg aatggttatg
     5041 ttctgtcata agattacctc ttttcttttt attattaaaa taattgctta cctgagccgt
     5101 ctaaataatt ttgtaaaatc atcacagctg ctaatttatc gatgactttt tttcgtttcg
     5161 ctcttgaagt atttgcctgc tctactagca tacgttctgc ctgaacagtg gttagtcgtt
     5221 catcttgata atcaaccggc aaggagaata gttctttgat cttctctcca tatgacatgg
     5281 aagcttctgc ccttggtccg atggaattgt tcatattctt aggtaatcca actacaaaac
     5341 ggtctacttc gtactgcgcc actaattcct tcaaccgatc aaaaccaaac tcttcttctg
     5401 cctcattgat tcgaatgatt tctactcctt gagctgtcca gccaaaggga tcgctcacag
     5461 caacgcctac tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg
     5521 atttgagagg tagtctttga caattacttc catgatttca tcgcgttcgt gacggcgaat
     5581 caaatttcgt gcatcacgat aacgaggaat gtaggccgag tctccagaaa gcaagtagcc
     5641 gacaatctga ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa
     5701 tgtttcgctc acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt
     5761 aaaacccatg attctacacc tctttcccgc aaggtaattc cagcttacct tctacaccct
     5821 tattttactc gaagaaaggg aaaactacaa acaaatattc caaacttctg aataaattaa
     5881 gaaacacgat ttgtctgatt tttccttttt tctgtggctt tttcaaaagc cctctttcca
     5941 taaagtatac gctatatttc tttgaaagta tgtttttaca caattttttc ataaagttca
     6001 taaaaaattc gaattgctgc tactaatttt tcgttttcgt tgcttgccct gtctcataat
     6061 tgatggtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
     6121 caatgctttc tgcttccaac tgcacacctc ttgctaccag ttcatttccc tcgaaatgag
     6181 gagtgacacg atagcgtaca tgatgatttg tttccttgat atatgccgca atatcatttt
     6241 cgtgggcaag catgtacgga gcattcaacg aacgtgtccc tgtcattaag tttttaatgt
     6301 tattattttg accagtcaac tggaaaccaa tcaaatggct gcggttgtat aaccattgtc
     6361 cgtcacttaa tttcttttgt ttccaacctg tcggatcaat atacaaaggt tctctctttt
     6421 cagtaggaaa caagtctttt cccaacatcg cattggcttg tcctactctg ttcaatttgt
     6481 ctatatttga atagctttcc catgtccctt tggaaagact aagatcctct tttgtaaact
     6541 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
     6601 ctacttgaga agcttgcggt gtggaatcaa aaatggttgt aatttgttgg aagatatctg
     6661 aatctttttg tgtggtggaa ataccgagtg cccctgcaat cacagcagcg acgatcccca
     6721 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ttccctaaat
     6781 cttaacatat gttcccctat aaataaatac tggaaagaaa tagaaacagt aaaaaaataa
     6841 aaccagtttt tagacggctt aagaatacaa aaaaagagac ggattcatcg tctctactca
     6901 atcacaaaac actatttaca aggtatttat tatcattcaa aaaatttttc ttaatactca
     6961 aaactaaacg gcttctgtac gacctcttgg taaacagtgt tcaaaagctt gcatctgcgg
     7021 taataagtcc aaccaagtca aaaacataag aagctttttt gcttgactat tcttattact
     7081 tgatgcaggg cagctttttc acagcctctt ttaagcgggt gacgggaatc gaacccgcga
     7141 catcagcttg ggaagctggg attttaccac taaactacac ccgcgataca aaaacagtat
     7201 acgcctcttt cactccttcc tcaagttttt ttattcttta cgcttccgat atgttttgta
     7261 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
     7321 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
     7381 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
     7441 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
     7501 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
     7561 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
     7621 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
     7681 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
     7741 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
     7801 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
     7861 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
     7921 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
     7981 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
     8041 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
     8101 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
     8161 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
     8221 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
     8281 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
     8341 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
     8401 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
     8461 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
     8521 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
     8581 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
     8641 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
     8701 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
     8761 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
     8821 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
     8881 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
     8941 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
     9001 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
     9061 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
     9121 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
     9181 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
     9241 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
     9301 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
     9361 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
     9421 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
     9481 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
     9541 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
     9601 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
     9661 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
     9721 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
     9781 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
     9841 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
     9901 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
     9961 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    10021 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    10081 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    10141 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    10201 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    10261 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    10321 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    10381 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    10441 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    10501 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    10561 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    10621 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    10681 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    10741 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    10801 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    10861 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    10921 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    10981 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    11041 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    11101 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    11161 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    11221 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    11281 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    11341 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    11401 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    11461 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    11521 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    11581 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    11641 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    11701 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    11761 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    11821 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    11881 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    11941 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    12001 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    12061 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    12121 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    12181 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    12241 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    12301 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    12361 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    12421 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    12481 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    12541 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    12601 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    12661 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    12721 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    12781 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    12841 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    12901 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    12961 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    13021 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    13081 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    13141 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    13201 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    13261 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    13321 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    13381 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    13441 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    13501 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    13561 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    13621 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    13681 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    13741 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    13801 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    13861 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    13921 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    13981 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    14041 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    14101 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    14161 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    14221 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    14281 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    14341 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    14401 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    14461 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    14521 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    14581 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    14641 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    14701 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    14761 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    14821 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    14881 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    14941 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    15001 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    15061 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    15121 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    15181 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    15241 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    15301 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    15361 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    15421 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    15481 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    15541 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    15601 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    15661 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    15721 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    15781 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    15841 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    15901 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    15961 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    16021 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    16081 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    16141 gacattttga ttctagacga accaacgaac catttagata tcgagacact ttcctggctt
    16201 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    16261 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    16321 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    16381 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    16441 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    16501 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    16561 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    16621 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    16681 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    16741 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    16801 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    16861 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    16921 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    16981 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    17041 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    17101 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    17161 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    17221 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    17281 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    17341 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    17401 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    17461 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    17521 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    17581 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    17641 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    17701 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    17761 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    17821 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    17881 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    17941 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    18001 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    18061 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    18121 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    18181 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    18241 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    18301 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    18361 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    18421 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    18481 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    18541 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    18601 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    18661 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    18721 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    18781 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    18841 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    18901 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    18961 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    19021 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    19081 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    19141 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    19201 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    19261 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    19321 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    19381 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    19441 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    19501 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    19561 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    19621 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    19681 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    19741 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    19801 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    19861 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    19921 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    19981 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    20041 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    20101 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    20161 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    20221 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    20281 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    20341 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    20401 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    20461 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    20521 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    20581 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    20641 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    20701 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    20761 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    20821 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    20881 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    20941 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    21001 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    21061 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    21121 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    21181 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    21241 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    21301 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    21361 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    21421 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    21481 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    21541 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    21601 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    21661 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    21721 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    21781 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    21841 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    21901 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    21961 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    22021 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    22081 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    22141 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    22201 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    22261 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    22321 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    22381 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    22441 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    22501 agctgaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    22561 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    22621 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    22681 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    22741 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    22801 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    22861 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    22921 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    22981 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    23041 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    23101 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    23161 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    23221 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    23281 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    23341 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    23401 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    23461 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    23521 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    23581 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    23641 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    23701 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    23761 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    23821 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    23881 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    23941 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    24001 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    24061 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    24121 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    24181 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    24241 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    24301 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    24361 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    24421 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    24481 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    24541 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    24601 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    24661 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    24721 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    24781 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    24841 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    24901 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    24961 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    25021 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    25081 ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt
    25141 tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt
    25201 ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc
    25261 catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag
    25321 cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt
    25381 attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt
    25441 tatcattatc cagttttatt ttgatgagca attattagca catattacgg gacaatcttt
    25501 ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat
    25561 tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca attctttttt
    25621 catactatgc caacagatga gcattcattt gctttcctta ttagttactt attgtctgca
    25681 aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat
    25741 accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt
    25801 tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt
    25861 agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg
    25921 aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct
    25981 agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca
    26041 tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt
    26101 aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat
    26161 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
    26221 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
    26281 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
    26341 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
    26401 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
    26461 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
    26521 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
    26581 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
    26641 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
    26701 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
    26761 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
    26821 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
    26881 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
    26941 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
    27001 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
    27061 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
    27121 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
    27181 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
    27241 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
    27301 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
    27361 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
    27421 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
    27481 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
    27541 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
    27601 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
    27661 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
    27721 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
    27781 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
    27841 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
    27901 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga
    27961 atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt
    28021 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
    28081 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
    28141 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
    28201 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
    28261 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
    28321 ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa
    28381 ataatttaat ttttttgttc ctatttattt a
//
LOCUS       DAJSNV010000026        27593 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSNV010000026 DAJSNV010000000
VERSION     DAJSNV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.027"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(114..1058)
                     /locus_tag="ON516_001136"
     CDS             complement(114..1058)
                     /locus_tag="ON516_001136"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3344629.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1354..2298)
                     /locus_tag="ON516_001137"
     CDS             complement(1354..2298)
                     /locus_tag="ON516_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344630.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2519..3733
                     /locus_tag="ON516_001138"
     CDS             2519..3733
                     /locus_tag="ON516_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3344631.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3755..5770
                     /locus_tag="ON516_001139"
     CDS             3755..5770
                     /locus_tag="ON516_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HCR3344632.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5853..7226)
                     /locus_tag="ON516_001140"
     CDS             complement(5853..7226)
                     /locus_tag="ON516_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3344633.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7252..7536)
                     /locus_tag="ON516_001141"
     CDS             complement(7252..7536)
                     /locus_tag="ON516_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3344634.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7538..9553)
                     /locus_tag="ON516_001142"
     CDS             complement(7538..9553)
                     /locus_tag="ON516_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3344635.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9732..10037)
                     /locus_tag="ON516_001143"
     CDS             complement(9732..10037)
                     /locus_tag="ON516_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3344636.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10126..11157)
                     /locus_tag="ON516_001144"
     CDS             complement(10126..11157)
                     /locus_tag="ON516_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3344637.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11364..12239
                     /locus_tag="ON516_001145"
     CDS             11364..12239
                     /locus_tag="ON516_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3344638.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12391..13233)
                     /locus_tag="ON516_001146"
     CDS             complement(12391..13233)
                     /locus_tag="ON516_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3344639.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13325..15202)
                     /locus_tag="ON516_001147"
     CDS             complement(13325..15202)
                     /locus_tag="ON516_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3344640.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15199..16620)
                     /locus_tag="ON516_001148"
     CDS             complement(15199..16620)
                     /locus_tag="ON516_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3344641.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16623..20012)
                     /locus_tag="ON516_001149"
     CDS             complement(16623..20012)
                     /locus_tag="ON516_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3344642.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20269..21660
                     /locus_tag="ON516_001150"
     CDS             20269..21660
                     /locus_tag="ON516_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344643.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21733..22380)
                     /locus_tag="ON516_001151"
     CDS             complement(21733..22380)
                     /locus_tag="ON516_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3344644.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22388..23074)
                     /locus_tag="ON516_001152"
     CDS             complement(22388..23074)
                     /locus_tag="ON516_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344645.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23080..24153)
                     /locus_tag="ON516_001153"
     CDS             complement(23080..24153)
                     /locus_tag="ON516_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344646.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24375..25049
                     /locus_tag="ON516_001154"
     CDS             24375..25049
                     /locus_tag="ON516_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3344647.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25054..26394
                     /locus_tag="ON516_001155"
     CDS             25054..26394
                     /locus_tag="ON516_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3344648.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26543..27529)
                     /locus_tag="ON516_001156"
     CDS             complement(26543..27529)
                     /locus_tag="ON516_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3344649.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8037 a         5224 c         5010 g         9322 t
ORIGIN      
        1 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
       61 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      121 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      181 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      241 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      301 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      361 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      421 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      481 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      541 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      601 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      661 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
      721 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
      781 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
      841 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
      901 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
      961 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1021 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1081 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1141 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1201 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1261 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1321 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1381 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1441 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1501 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1561 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1621 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1681 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     1741 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     1801 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     1861 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     1921 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     1981 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2041 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2101 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2161 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2221 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2281 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2341 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2401 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2461 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2521 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2581 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2641 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     2701 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     2761 tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     2821 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     2881 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     2941 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3001 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3061 tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac aagtaatcaa
     3121 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3181 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3241 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3301 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3361 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3421 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3481 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3541 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3601 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3661 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     3721 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     3781 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     3841 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     3901 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     3961 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4021 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4081 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4141 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4201 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4261 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4321 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4381 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4441 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4501 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4561 atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4621 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4681 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     4741 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     4801 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     4861 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     4921 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     4981 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5041 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5101 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5161 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5221 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5281 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5341 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5401 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5461 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5521 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5581 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5641 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     5701 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     5761 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     5821 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     5881 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     5941 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6001 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6061 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6121 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6181 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6241 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6301 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6361 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6421 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6481 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6541 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6601 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6661 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     6721 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     6781 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     6841 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     6901 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     6961 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7021 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7081 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7141 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7201 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7261 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7321 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7381 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7441 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7501 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7561 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7621 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7681 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     7741 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     7801 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     7861 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     7921 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     7981 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8041 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8101 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8161 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8221 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8281 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8341 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8401 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8461 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8521 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8581 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8641 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     8701 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     8761 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     8821 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     8881 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     8941 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9001 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9061 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9121 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9181 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9241 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9301 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9361 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9421 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9481 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9541 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9601 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9661 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
     9721 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
     9781 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
     9841 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
     9901 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
     9961 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10021 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10081 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10141 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10201 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10261 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10321 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10381 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10441 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10501 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10561 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10621 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10681 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    10741 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    10801 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    10861 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    10921 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    10981 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11041 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11101 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11161 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11221 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11281 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11341 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11401 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11461 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11521 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11581 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11641 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    11701 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    11761 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    11821 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    11881 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    11941 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12001 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12061 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12121 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12181 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12241 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12301 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12361 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12421 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12481 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12541 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12601 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12661 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    12721 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    12781 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    12841 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    12901 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    12961 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13021 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13081 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13141 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13201 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13261 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13321 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13381 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13441 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13501 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13561 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13621 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13681 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    13741 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    13801 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    13861 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    13921 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    13981 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    14041 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    14101 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14161 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14221 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14281 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14341 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14401 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14461 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14521 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14581 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14641 agactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    14701 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    14761 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    14821 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    14881 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    14941 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15001 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15061 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15121 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15181 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15241 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15301 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15361 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15421 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15481 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15541 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15601 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15661 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    15721 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    15781 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    15841 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    15901 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    15961 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16021 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16081 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16141 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16201 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16261 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16321 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16381 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16441 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16501 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16561 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16621 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16681 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    16741 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    16801 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    16861 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    16921 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    16981 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17041 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17101 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17161 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17221 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17281 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17341 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17401 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17461 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17521 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17581 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17641 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    17701 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    17761 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    17821 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    17881 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    17941 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18001 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18061 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18121 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18181 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18241 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18301 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18361 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18421 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18481 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18541 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18601 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18661 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    18721 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    18781 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    18841 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    18901 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    18961 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19021 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19081 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19141 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19201 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19261 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19321 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19381 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19441 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19501 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19561 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19621 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19681 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    19741 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    19801 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    19861 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    19921 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    19981 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20041 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20101 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20161 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20221 gagggagaaa aacatatgat caggttgttg gaatgcaact atagaaagat ggaaaaaatc
    20281 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20341 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20401 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20461 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20521 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20581 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20641 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    20701 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    20761 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    20821 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    20881 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    20941 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21001 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21061 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21121 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21181 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21241 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21301 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21361 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21421 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21481 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21541 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21601 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21661 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    21721 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    21781 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    21841 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    21901 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    21961 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22021 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22081 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22141 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22201 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22261 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22321 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22381 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22441 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22501 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22561 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22621 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22681 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    22741 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    22801 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    22861 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    22921 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    22981 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23041 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23101 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23161 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23221 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23281 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23341 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23401 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23461 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23521 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23581 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23641 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    23701 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    23761 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    23821 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    23881 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    23941 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24001 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24061 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24121 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24181 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24241 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24301 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24361 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24421 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24481 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24541 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24601 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24661 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    24721 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    24781 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    24841 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    24901 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    24961 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25021 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25081 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25141 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25201 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25261 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25321 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25381 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25441 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25501 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25561 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25621 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25681 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    25741 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    25801 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    25861 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    25921 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    25981 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26041 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26101 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26161 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26221 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26281 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26341 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26401 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26461 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26521 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26581 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26641 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    26701 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    26761 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    26821 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    26881 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    26941 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27001 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27061 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27121 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27181 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27241 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27301 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27361 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27421 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27481 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27541 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta tag
//
LOCUS       DAJSNV010000027        26922 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSNV010000027 DAJSNV010000000
VERSION     DAJSNV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.028"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..1091
                     /locus_tag="ON516_001157"
     CDS             87..1091
                     /locus_tag="ON516_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344650.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1092..2036
                     /locus_tag="ON516_001158"
     CDS             1092..2036
                     /locus_tag="ON516_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344651.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2036..2998
                     /locus_tag="ON516_001159"
     CDS             2036..2998
                     /locus_tag="ON516_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344652.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3025..3939
                     /locus_tag="ON516_001160"
     CDS             3025..3939
                     /locus_tag="ON516_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344653.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3965..5746
                     /locus_tag="ON516_001161"
     CDS             3965..5746
                     /locus_tag="ON516_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3344654.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6094..6780
                     /locus_tag="ON516_001162"
     CDS             6094..6780
                     /locus_tag="ON516_001162"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3344655.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6800..10381
                     /gene="smc"
                     /locus_tag="ON516_001163"
     CDS             6800..10381
                     /gene="smc"
                     /locus_tag="ON516_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3344656.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10390..11199
                     /locus_tag="ON516_001164"
     CDS             10390..11199
                     /locus_tag="ON516_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3344657.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11210..12208
                     /gene="ftsY"
                     /locus_tag="ON516_001165"
     CDS             11210..12208
                     /gene="ftsY"
                     /locus_tag="ON516_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3344658.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12395..14662
                     /gene="gshAB"
                     /locus_tag="ON516_001166"
     CDS             12395..14662
                     /gene="gshAB"
                     /locus_tag="ON516_001166"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3344659.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(14751..15203)
                     /locus_tag="ON516_001167"
     CDS             complement(14751..15203)
                     /locus_tag="ON516_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3344660.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15353..16303
                     /locus_tag="ON516_001168"
     CDS             15353..16303
                     /locus_tag="ON516_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3344661.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16296..17255
                     /locus_tag="ON516_001169"
     CDS             16296..17255
                     /locus_tag="ON516_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3344662.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17252..18007
                     /locus_tag="ON516_001170"
     CDS             17252..18007
                     /locus_tag="ON516_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3344663.1"
                     /translation="MIDLQKINKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18030..18983
                     /locus_tag="ON516_001171"
     CDS             18030..18983
                     /locus_tag="ON516_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3344664.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19627..19923)
                     /locus_tag="ON516_001172"
     CDS             complement(19627..19923)
                     /locus_tag="ON516_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR3344665.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20278..20634
                     /locus_tag="ON516_001173"
     CDS             20278..20634
                     /locus_tag="ON516_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3344666.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20644..21543
                     /locus_tag="ON516_001174"
     CDS             20644..21543
                     /locus_tag="ON516_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3344667.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22060..22791
                     /locus_tag="ON516_001175"
     CDS             22060..22791
                     /locus_tag="ON516_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3344668.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22936..24963
                     /locus_tag="ON516_001176"
     CDS             22936..24963
                     /locus_tag="ON516_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3344669.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25071..26168
                     /locus_tag="ON516_001177"
     CDS             25071..26168
                     /locus_tag="ON516_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3344670.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26475..>26922
                     /locus_tag="ON516_001178"
     CDS             26475..>26922
                     /locus_tag="ON516_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344671.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         9024 a         4662 c         5686 g         7550 t
ORIGIN      
        1 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
       61 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
      121 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
      181 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
      241 tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt
      301 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
      361 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
      421 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
      481 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
      541 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
      601 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
      661 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
      721 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
      781 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
      841 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
      901 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
      961 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
     1021 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
     1081 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
     1141 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
     1201 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
     1261 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
     1321 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
     1381 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
     1441 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
     1501 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
     1561 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
     1621 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
     1681 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
     1741 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
     1801 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
     1861 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
     1921 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
     1981 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
     2041 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
     2101 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
     2161 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
     2221 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
     2281 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
     2341 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
     2401 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
     2461 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
     2521 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
     2581 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
     2641 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
     2701 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
     2761 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
     2821 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
     2881 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
     2941 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
     3001 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
     3061 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
     3121 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
     3181 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
     3241 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
     3301 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
     3361 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
     3421 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
     3481 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
     3541 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
     3601 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
     3661 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
     3721 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
     3781 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
     3841 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
     3901 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
     3961 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
     4021 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
     4081 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
     4141 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
     4201 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
     4261 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
     4321 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
     4381 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
     4441 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
     4501 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
     4561 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
     4621 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
     4681 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
     4741 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
     4801 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
     4861 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
     4921 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
     4981 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
     5041 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
     5101 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
     5161 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
     5221 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
     5281 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
     5341 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
     5401 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
     5461 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
     5521 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
     5581 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
     5641 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
     5701 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
     5761 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
     5821 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
     5881 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
     5941 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
     6001 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
     6061 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
     6121 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
     6181 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
     6241 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
     6301 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
     6361 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
     6421 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
     6481 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
     6541 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
     6601 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
     6661 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
     6721 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
     6781 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
     6841 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
     6901 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
     6961 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
     7021 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
     7081 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
     7141 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
     7201 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
     7261 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
     7321 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
     7381 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
     7441 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
     7501 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
     7561 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
     7621 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
     7681 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
     7741 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
     7801 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
     7861 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
     7921 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
     7981 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
     8041 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
     8101 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
     8161 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
     8221 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
     8281 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
     8341 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
     8401 cacgttagct atcgaagcag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
     8461 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
     8521 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
     8581 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
     8641 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
     8701 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
     8761 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
     8821 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
     8881 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
     8941 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
     9001 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
     9061 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
     9121 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
     9181 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
     9241 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
     9301 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
     9361 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
     9421 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
     9481 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
     9541 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
     9601 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
     9661 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
     9721 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
     9781 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
     9841 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
     9901 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
     9961 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    10021 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    10081 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    10141 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    10201 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    10261 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    10321 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    10381 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    10441 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    10501 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    10561 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    10621 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    10681 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    10741 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    10801 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    10861 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    10921 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    10981 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    11041 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    11101 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    11161 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    11221 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    11281 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    11341 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    11401 cggtgcagat gtaggttttg aaacagcgat acgaatcacc gaagcattac gtcaagaagt
    11461 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    11521 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    11581 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    11641 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    11701 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    11761 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    11821 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    11881 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    11941 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    12001 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    12061 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    12121 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    12181 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    12241 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    12301 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    12361 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    12421 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    12481 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    12541 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    12601 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    12661 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    12721 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    12781 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    12841 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    12901 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    12961 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    13021 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    13081 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    13141 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    13201 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    13261 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    13321 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    13381 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    13441 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    13501 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    13561 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    13621 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    13681 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    13741 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    13801 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    13861 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    13921 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    13981 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    14041 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    14101 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    14161 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    14221 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    14281 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    14341 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    14401 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    14461 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    14521 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    14581 tatgcatgtt tatccttttg caggaaaaga cagaagattg acgatgaatg tattgaaatt
    14641 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    14701 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    14761 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    14821 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    14881 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    14941 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    15001 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    15061 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    15121 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    15181 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    15241 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    15301 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    15361 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    15421 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    15481 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    15541 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    15601 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    15661 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    15721 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    15781 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    15841 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    15901 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    15961 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    16021 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    16081 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    16141 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    16201 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    16261 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    16321 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    16381 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    16441 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    16501 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    16561 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    16621 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    16681 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    16741 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    16801 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    16861 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    16921 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    16981 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    17041 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    17101 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    17161 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    17221 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaaga tcaataaact
    17281 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaagttgac
    17341 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    17401 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    17461 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    17521 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    17581 aaaagacgag gaaatcgttc aggaatcact ggaacacctt gggttagaag aaatagcgga
    17641 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    17701 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    17761 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    17821 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    17881 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    17941 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    18001 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    18061 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    18121 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    18181 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    18241 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    18301 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    18361 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    18421 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    18481 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    18541 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    18601 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    18661 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    18721 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    18781 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    18841 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    18901 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    18961 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    19021 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    19081 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    19141 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    19201 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    19261 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    19321 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    19381 aagctatttt ctgtccagcg ttcttattac tcaaaacaaa tcgcagttgt cacaatctct
    19441 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    19501 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    19561 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    19621 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    19681 aaaatcggcg ggaagcttac tccaccgttt tagtaattga tagaaataac tctcttctgc
    19741 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    19801 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    19861 ataagaaccc tcgatatttt gtgtttcttc gataaaatag atgtttttta tatattcctt
    19921 catagtgatt cctccaaaga agtcttttat ttgataagtt tactctaccg aagagttgtt
    19981 aataatccgt gaacaaaaag taattctttt tccaatagtt gttaaaggtt ttttattttt
    20041 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
    20101 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
    20161 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
    20221 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
    20281 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
    20341 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
    20401 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
    20461 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
    20521 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
    20581 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
    20641 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
    20701 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
    20761 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
    20821 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
    20881 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
    20941 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
    21001 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
    21061 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
    21121 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
    21181 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
    21241 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
    21301 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
    21361 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
    21421 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
    21481 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
    21541 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
    21601 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
    21661 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
    21721 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
    21781 gattgaagtc taggcagtca agtcacattt tgatagatag gagggggact tacgaattat
    21841 tttaaaggct atgacagctg tatttctaga ttgtgtttgc atcaagttgc aataaaaact
    21901 agaaatcaaa tcgggctgat aatcatttat aactaatcga tacataggac caaatatagg
    21961 tctatgttct tttatttcat aagtatcgta aaacagaatt ttgctatgga ttaatccaat
    22021 cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta
    22081 ttaacagcag caatgatgtt attcagcatg gtagcaagta cgacttttgt ttctgcaact
    22141 gaagtatacc caaaggaata taacacagaa ggaacgatta catttgaagc aggagatgag
    22201 ggtgtaactc ctcctgttga tccagagaat cctgatccca ataaacctgt cgatccatca
    22261 gatcctccaa gccctggaac aggaggggca ttatcgattg attatggatc aaaattcaaa
    22321 tttgggactc aaaaaatttc tactgcggat aaaacgtatt atgcagctgc tgatgtgatg
    22381 aacgatgggt ctagaaaacc gacatatgtc caagtgacag ataggagaag taccttatct
    22441 gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta
    22501 gttggagcac agctgaaatt tacgaaaggg caggctgttt ctttggttga tccaacatat
    22561 acgccgcaaa cggtgaattc tgaattaacg ttgacaccgg gaggaaacaa tacgttagca
    22621 attaatgcta aatcaggaac aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa
    22681 aatgaaaatc aagatgcggt acagctttcg gtacccggta aatctgtcaa gttagcgcaa
    22741 caatattcca ctaaattagt ttggacatta gaagatacac ctaacaacta gtttactgaa
    22801 aagaactgtt aaaaataaat acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt
    22861 gtcaaaggct aggctaaatt tgtttggata cgaatagttc atctaggaat ttaacgatca
    22921 ggaggaaaag atatattgaa aaagaggagg atcgtgctca ttttaagcag tctcttagtg
    22981 actaatatct ttccgatcat agggtactct ttggaattga atcagttgga gcatggaaat
    23041 agaagagacg aagaaagcaa actcacttcg acattcgaca gtttaataga atccaaccag
    23101 acacaagaaa gcagtgagga tttaagtatt tcgagtagtg gagagaccac tgacaacgta
    23161 gaagctgagg gttcggacaa acaaggacta gaagatgcac caatttctgc ggttgagctg
    23221 cctgacgatc ccaatattat ttctatcgat aaaatttttc aaacacctat cggtactagc
    23281 acgtccatat tggaaaatgg gaagttactg cagttgaacc cagcagaaag gtctcaaaga
    23341 ggagctattt ggtcaaataa gaagattagt ttactctcgg attttacatt taaatcttat
    23401 ttatacttag gaaatcaacg agatagagcg ggagatggca tgacctttac attgactggc
    23461 gatcctagaa tggctagtaa tcccagtcaa gttattggat cgccaggtat gggaattggc
    23521 tcatattcta ctcgttctgg ccaaccttac attgcgaatg cgctctcgat tgagtttgat
    23581 acgtataaaa atagtggttc ttctgatcga atggatcgag agataactgc tgataataga
    23641 gggcatggac atgtcgcttt tgtcacgcct aaaccaaata ataataatta ttctggagag
    23701 catactggcg tgacggtagc tcctacattt ctttctaatg gcacatggcg cttgttaata
    23761 attaaatggg acgcggagca aagaagtctt acttatgatt tggaaggtgt cggtcgctct
    23821 tctcggatta tacctgatct cattaggcag tttggttcta cagaagtaaa ttgggggttt
    23881 acttcttcaa caggtacttt ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg
    23941 aatgtcacat caacagctga tgtaaaagtc aacgatagtg attatggggc catcgctgaa
    24001 gctggaggaa acgataagat aactatcaga aatacattga aggtcgaggg ggattttctt
    24061 gaatataaga aaatgcagac tagtatcgac ctcccacaag aattaactat tcccgagcat
    24121 atcttgatca atggaaatca aattgatgcc aaagatatct tgataaccgg aaatcgagtc
    24181 acgctaaatc tgacagatta ttttattgct ggagatacgt taataatcga attggagaca
    24241 actttgcagg aagtaattcc tgaaaaagta ctcactatgc attttgaata ccttgaaaac
    24301 gggaatttga ttgagcaatc gaatgaaata aacctaaaga tcgcaaagct gaaggagata
    24361 gatatccatg tttattataa agataaagac agtcagattg atattgctga aatgaaaaca
    24421 atcactggga aaataggaga gaagtataaa gaaactccta ttgatattga agggtatgtt
    24481 tttgactcgg attcagcgaa tacagaaggc atcttctccg aaaatacaga ggatatctat
    24541 ttttattaca gaaaaggcaa actttatctc tcaactgctc ctcaatatgt caattttgga
    24601 aaacataaaa tatccagtag accattaagt gaattcggac agttttcagg tgggttgaaa
    24661 attatagatg aacgggcttt tggaagttgg aaattacaat tgaaacaaga gaaagttctt
    24721 acaaatggag attttgagct tccagaagct ctttcctttg ttacctctga aaatacaacg
    24781 gttataggga catccgctgt tacgattttc gaaagtgatc aaaagggtga aagcgatttg
    24841 tcctctcttt tagattctac agctcatcgt ggaatcagaa taaatattcc tgtagaaaac
    24901 caaagaatcg gaacctttga ggggaaacta tcttggatac ttgcagatgt cccaggaaat
    24961 taacaggaag agaagaatat aacaaaatta ttgagtttca agaagggcac aggtaccata
    25021 ggctcttgtg tcccacagag acaagatttt gttatagtct tctcttacat atgattaaag
    25081 gagcgatttt cttgagacca aaacaacaat tatttataac actcctagtt attttagggg
    25141 cactttttaa caatatatca gtgtcagcta gtgcatttaa tttttcagtg acgccgataa
    25201 cttcagagaa tcaaatcgac aagagaaaaa cttacttcga tcttcaactt gatccggatc
    25261 aggaagtaga agtaaaagcg gaattgagaa atgatacaga aaaagaagtg aaaatcgata
    25321 tttctgtgaa tagcgcaacc accaattcaa atgtgatggt tgaatacgga aaaaacgaga
    25381 ttgaaaaaga catgagcctg atctttgatc tggtagatta tgtgagctat ccacagatag
    25441 tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc
    25501 gatttgatgg tgtattggct ggtgggatca cattccaaga aatccagacg gaaaaggacc
    25561 aaacagaaac taaggatcaa agcttatcga ttgagaatga atacacctat attattgctt
    25621 tgttgatgca acagacactg aacgaagttg ctccgaacct tactcttcat gaagttaaac
    25681 cagatcaaat caatgcaaga aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct
    25741 atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa aggacattct gaagttttat
    25801 atcaagagga aaaagaagga ttgcagattg cgccgaatac aaatttttct tttccaactg
    25861 ctttaaatgg tcaaccgcta accccaggtg aatatcacct tacaatgact gttttaggaa
    25921 atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa tactacgatc aactatacca
    25981 atcaatgggt atttgaaaaa gattttacaa tagacggaaa agtagccaag gaattgaaca
    26041 cgacggatgt tacgattaaa aaagacaata attggattta tatcttagta gcttcacttt
    26101 tcttattgtt tattttatta tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag
    26161 aaaagtaagg ttaagataac aaatagtaaa tttttattat tgtaggatgg ttgggctctt
    26221 tttataacaa tataaaatat accattttat taataaaaat gcatactagt aaatgatata
    26281 attctaataa caggtttgat cgcgagaaag gccaaataaa acgctctcca aaatgttaaa
    26341 aaaaagacag gacgatgtat attatacaaa caagaatcgc tgacaacagt gactcggatc
    26401 caggaaatca gttttatccc cagctagttg gtgaatgcat ggaaagtaat atacgtagaa
    26461 agagctgatg actaatgaat tatgagtttt ttttaagcaa agaagatcac agaagttatt
    26521 cattacttaa atatttagaa gcaagctcaa atttatcaga gtcaatatcg aatgttcaag
    26581 aagagttgtc gctatcgaca tttctcttga aaaaaacgat cgataagctg aaacatgatt
    26641 tacaaaagtt cgatctagag aaaaatttgc gagtaaccgt cagtgaattg gatatatgtc
    26701 tggaaattaa tggaaaatgt tctagcaaag ctctgttatc tcgttatata acggattctt
    26761 tatctatgaa aatggtattg gctttctttc aggaaaaata tgtctcagta gaaaattttg
    26821 ctaaacagca tcacgtgagt tattctgtgg cttataaagt attacaaaga ctaaaaagaa
    26881 atctaaaaaa atatcaaatt ttttttgaaa agagaaagtt ga
//
LOCUS       DAJSNV010000028        26367 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSNV010000028 DAJSNV010000000
VERSION     DAJSNV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.029"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..203)
                     /locus_tag="ON516_001179"
     CDS             complement(<1..203)
                     /locus_tag="ON516_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344672.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYF"
     gene            complement(452..652)
                     /locus_tag="ON516_001180"
     CDS             complement(452..652)
                     /locus_tag="ON516_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3344673.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(957..2189)
                     /locus_tag="ON516_001181"
     CDS             complement(957..2189)
                     /locus_tag="ON516_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3344674.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2443..3012
                     /locus_tag="ON516_001182"
     CDS             2443..3012
                     /locus_tag="ON516_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3344675.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3190..3630)
                     /locus_tag="ON516_001183"
     CDS             complement(3190..3630)
                     /locus_tag="ON516_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3344676.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3788..4552
                     /gene="map"
                     /locus_tag="ON516_001184"
     CDS             3788..4552
                     /gene="map"
                     /locus_tag="ON516_001184"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3344677.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4584..5507
                     /locus_tag="ON516_001185"
     CDS             4584..5507
                     /locus_tag="ON516_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3344678.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5583..6722
                     /locus_tag="ON516_001186"
     CDS             5583..6722
                     /locus_tag="ON516_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3344679.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6715..7515
                     /locus_tag="ON516_001187"
     CDS             6715..7515
                     /locus_tag="ON516_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3344680.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7515..8342
                     /locus_tag="ON516_001188"
     CDS             7515..8342
                     /locus_tag="ON516_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3344681.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8320..9054
                     /locus_tag="ON516_001189"
     CDS             8320..9054
                     /locus_tag="ON516_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3344682.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9154..10020
                     /gene="rfbA"
                     /locus_tag="ON516_001190"
     CDS             9154..10020
                     /gene="rfbA"
                     /locus_tag="ON516_001190"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3344683.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10034..10606
                     /gene="rfbC"
                     /locus_tag="ON516_001191"
     CDS             10034..10606
                     /gene="rfbC"
                     /locus_tag="ON516_001191"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3344684.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10628..11656
                     /gene="rfbB"
                     /locus_tag="ON516_001192"
     CDS             10628..11656
                     /gene="rfbB"
                     /locus_tag="ON516_001192"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3344685.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11754..12605
                     /gene="rfbD"
                     /locus_tag="ON516_001193"
     CDS             11754..12605
                     /gene="rfbD"
                     /locus_tag="ON516_001193"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3344686.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12640..14673
                     /locus_tag="ON516_001194"
     CDS             12640..14673
                     /locus_tag="ON516_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344687.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14717..15997
                     /locus_tag="ON516_001195"
     CDS             14717..15997
                     /locus_tag="ON516_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3344688.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16207..17013
                     /locus_tag="ON516_001196"
     CDS             16207..17013
                     /locus_tag="ON516_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344689.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17025..18245
                     /locus_tag="ON516_001197"
     CDS             17025..18245
                     /locus_tag="ON516_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344690.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18235..19821
                     /locus_tag="ON516_001198"
     CDS             18235..19821
                     /locus_tag="ON516_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3344691.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19860..21998
                     /locus_tag="ON516_001199"
     CDS             19860..21998
                     /locus_tag="ON516_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3344692.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22366..23349)
                     /locus_tag="ON516_001200"
     CDS             complement(22366..23349)
                     /locus_tag="ON516_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3344693.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23727..25118
                     /locus_tag="ON516_001201"
     CDS             23727..25118
                     /locus_tag="ON516_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3344694.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25134..26174
                     /locus_tag="ON516_001202"
     CDS             25134..26174
                     /locus_tag="ON516_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3344695.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26193..>26367
                     /locus_tag="ON516_001203"
     CDS             26193..>26367
                     /locus_tag="ON516_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3344696.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGR"
BASE COUNT         8396 a         4454 c         5349 g         8168 t
ORIGIN      
        1 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
       61 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
      121 ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt aaaccataat
      181 tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtatacg aacgaatgtt
      241 ctttttgtga aggcggaatt taatatactg aataatagaa aacaagtact tgtgccaaat
      301 tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa atagaaaacc ttctatatta
      361 agatttcctg caataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa
      421 ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt
      481 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
      541 aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa
      601 aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat
      661 agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga aagatttatc
      721 gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga
      781 gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc
      841 aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag
      901 cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa
      961 gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat tccgtcgata
     1021 tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt caatccagct
     1081 tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc ataagcagaa
     1141 cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa gatgatgcca
     1201 gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct
     1261 tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga aaaatcaaca
     1321 acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc ataactcagt
     1381 ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt gaacacttct
     1441 tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc agcgccttcc
     1501 caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt atagtgaagg
     1561 gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc atcatgcttc
     1621 ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat ctgatcccat
     1681 aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc ccattgttta
     1741 ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt gcgtaaattc
     1801 attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc tacaccagca
     1861 aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc aagccattca
     1921 tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa
     1981 agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc ggctcctagt
     2041 ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct gatctgtaaa
     2101 acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg tgcatatttt
     2161 tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca ttttttctgg
     2221 taactattag catgatacca tttaattatg attcaaacaa tgaggcttta aataaaaaaa
     2281 ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct atttcagctt
     2341 gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat gaaattaatc
     2401 aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag aaaaaagacg
     2461 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     2521 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     2581 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     2641 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     2701 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtgctt agaagaatat
     2761 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     2821 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     2881 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     2941 cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa agaaccaatc
     3001 gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa
     3061 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     3121 catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg ctcttttatt
     3181 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     3241 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     3301 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     3361 agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt cttcataaaa
     3421 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     3481 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     3541 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     3601 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     3661 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga
     3721 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     3781 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     3841 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     3901 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     3961 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     4021 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     4081 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     4141 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     4201 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     4261 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     4321 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     4381 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     4441 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     4501 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     4561 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     4621 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     4681 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     4741 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     4801 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     4861 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     4921 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     4981 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     5041 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     5101 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     5161 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     5221 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     5281 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     5341 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     5401 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     5461 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     5521 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     5581 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     5641 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     5701 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     5761 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     5821 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     5881 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     5941 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     6001 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     6061 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     6121 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     6181 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     6241 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     6301 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     6361 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     6421 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     6481 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     6541 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     6601 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     6661 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     6721 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     6781 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     6841 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     6901 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     6961 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     7021 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     7081 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     7141 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     7201 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     7261 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     7321 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     7381 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     7441 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     7501 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     7561 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     7621 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     7681 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     7741 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     7801 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     7861 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     7921 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     7981 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     8041 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     8101 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     8161 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     8221 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     8281 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     8341 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
     8401 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
     8461 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
     8521 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
     8581 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
     8641 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
     8701 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
     8761 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
     8821 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
     8881 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
     8941 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
     9001 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
     9061 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
     9121 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
     9181 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
     9241 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
     9301 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
     9361 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
     9421 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
     9481 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
     9541 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
     9601 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
     9661 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
     9721 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
     9781 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
     9841 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
     9901 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
     9961 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    10021 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    10081 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    10141 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    10201 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    10261 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    10321 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    10381 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    10441 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    10501 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    10561 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    10621 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    10681 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    10741 tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg
    10801 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    10861 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    10921 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    10981 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    11041 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    11101 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    11161 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    11221 tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg
    11281 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    11341 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    11401 gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt
    11461 aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga
    11521 gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    11581 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    11641 caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg
    11701 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt
    11761 tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa
    11821 atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa
    11881 tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag
    11941 tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa
    12001 gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact
    12061 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    12121 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    12181 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    12241 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    12301 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    12361 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag
    12421 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    12481 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg
    12541 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    12601 agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    12661 gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg
    12721 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    12781 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    12841 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
    12901 acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca
    12961 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
    13021 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    13081 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    13141 aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    13201 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt
    13261 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    13321 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    13381 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    13441 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    13501 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    13561 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    13621 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    13681 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    13741 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    13801 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    13861 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    13921 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    13981 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
    14041 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
    14101 tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc
    14161 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    14221 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    14281 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    14341 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    14401 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    14461 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    14521 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    14581 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    14641 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    14701 attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat
    14761 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt
    14821 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    14881 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    14941 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    15001 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    15061 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    15121 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    15181 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    15241 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg
    15301 tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta gaggtgcatt
    15361 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    15421 tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga tttcttatct
    15481 ggcggctgtc tctgaaaaag tcaatttgtt taccgatcga gaagatattt ggcataaatt
    15541 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    15601 ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    15661 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    15721 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    15781 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    15841 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    15901 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    15961 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    16021 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    16081 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    16141 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    16201 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    16261 ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    16321 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    16381 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    16441 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    16501 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    16561 ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata
    16621 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    16681 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    16741 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    16801 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    16861 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    16921 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    16981 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    17041 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    17101 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    17161 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    17221 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    17281 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    17341 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    17401 aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat caacctgtaa
    17461 aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc
    17521 ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt caactaaaat
    17581 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    17641 atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg
    17701 tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta aagaaaaaat
    17761 cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct ccagatatcg
    17821 tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat atgctggaac
    17881 aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca atcaaaaatc
    17941 cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga cttaatactc
    18001 tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat ctggagtatg
    18061 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    18121 caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc ggttcttata
    18181 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    18241 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    18301 aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac gaaacattta
    18361 atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt gttcgatttt
    18421 gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga gtgggcaaac
    18481 tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga acatttggta
    18541 gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg agagatcctg
    18601 attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa taaccaattg
    18661 ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac tcatgatggc
    18721 ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta tttgtactta
    18781 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgctat
    18841 gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt gatgaaacat
    18901 ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa agtgttagaa
    18961 gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa taatttcaca
    19021 ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc
    19081 ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga aaaactaaca
    19141 tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa
    19201 gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat ccattgtcac
    19261 tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac gattcgtccc
    19321 atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag agaaaatcat
    19381 catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac aaatcgctat
    19441 tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg acgtaagaaa
    19501 gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga gaaaatctgg
    19561 gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc ggatcgccag
    19621 cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat tcctgtttca
    19681 cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg atttgagtta
    19741 cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca aaatggccaa
    19801 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    19861 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    19921 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    19981 tagaaaaaga aaatgtgatt gctgaatcaa cacatgtcgt aagattggat atcaatcatt
    20041 tatataatct tgatgttaag acccaaagcg gtttcaaggt acgtttatca gggaaaatga
    20101 gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata gctgtgaaat
    20161 taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    20221 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg
    20281 tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    20341 aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt ccattgattt
    20401 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    20461 tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct tctactgatc
    20521 cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt
    20581 tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa atagcagaag
    20641 gagaatttat agcactctta gataatgatg atgaattacc gccatttgca ttatatgaag
    20701 tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac gaagataaaa
    20761 tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg cctgatacat
    20821 tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt gttaaagaac
    20881 ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga
    20941 cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat cattggcgga
    21001 cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat gattccggag
    21061 tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca
    21121 gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg gtcagtgtga
    21181 tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    21241 aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca gatccaaaaa
    21301 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc gtagaattga
    21361 ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa gcaaacggca
    21421 aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg atgacggtga
    21481 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg ttttatccag
    21541 atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca gggcatgcat
    21601 tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt gacgtcaatt
    21661 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg
    21721 ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    21781 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    21841 caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa
    21901 tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa
    21961 aagacggaat cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg
    22021 ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt
    22081 gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga gaaataagaa
    22141 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta agaagaagtt
    22201 gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt tcttactttt
    22261 gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag ccaaagtttc
    22321 acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt ttaaaataaa
    22381 tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt taaaataaga
    22441 agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc cactattacc
    22501 aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc catatctcat
    22561 attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta tccacatggg
    22621 tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac caatttttgc
    22681 cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta gtggatcaca
    22741 aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt cttcagctgt
    22801 ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat acttattggc
    22861 aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata tgtgtaaaca
    22921 gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata aatctagtga
    22981 tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt tacatttgta
    23041 gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt tctccccgcg
    23101 tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt tgattaatcc
    23161 ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg acatgatatg
    23221 acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta ccccatcctt
    23281 atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat gcaactcttg
    23341 tttattcata agatattacc tcactttctt ctataagcat aatgtcatta aaaatcgatt
    23401 tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca gcgctttggg
    23461 aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa aaagcagcaa
    23521 atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg gagatttgta
    23581 atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca tttttaaatt
    23641 ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga taatagagat
    23701 attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt gctgcacgac
    23761 ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat agtagtttct
    23821 tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa cactcagcaa
    23881 ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat gttttttata
    23941 atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg atgacactag
    24001 ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt gttatcttac
    24061 tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta tataaacttt
    24121 atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac
    24181 cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc
    24241 ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg
    24301 cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa
    24361 aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca aatattatga
    24421 atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct gaatatgaac
    24481 tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt gcttcaccat
    24541 ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc atctataaac
    24601 aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc tcgatgaccg
    24661 ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca cgagtaacgc
    24721 ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc
    24781 tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt
    24841 tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt atcactgggt
    24901 atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt tttgatttac
    24961 tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa accatcaaga
    25021 tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg
    25081 cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca
    25141 atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt ggtcgaacga
    25201 ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta aatgatggat
    25261 caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa cgttttgaat
    25321 tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata agacagtcaa
    25381 aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag
    25441 attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt tcgtttgttt
    25501 ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc
    25561 actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat gaacagttga
    25621 tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac caaattctat
    25681 ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat gaatactgtc
    25741 atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt gggaagggga
    25801 tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat tcattagcaa
    25861 atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta aaagggacaa
    25921 cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag aagaaagacg
    25981 aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt gctaagacgg
    26041 attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca ctatttttat
    26101 ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg gctgctttga
    26161 agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat tttcagggac
    26221 gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa attgatcgta
    26281 caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa gactatcatt
    26341 cagagattga aagattagga ggccgta
//
LOCUS       DAJSNV010000029        26152 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSNV010000029 DAJSNV010000000
VERSION     DAJSNV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.030"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            132..3659
                     /locus_tag="ON516_001204"
     CDS             132..3659
                     /locus_tag="ON516_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3344697.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="ON516_001205"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="ON516_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3344698.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="ON516_001206"
     CDS             7447..7788
                     /locus_tag="ON516_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344699.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="ON516_001207"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="ON516_001207"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3344700.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="ON516_001208"
     CDS             9145..11565
                     /locus_tag="ON516_001208"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3344701.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ON516_001209"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ON516_001209"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3344702.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="ON516_001210"
     CDS             12999..13736
                     /locus_tag="ON516_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3344703.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="ON516_001211"
     CDS             13750..14565
                     /locus_tag="ON516_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3344704.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="ON516_001212"
     CDS             14578..15225
                     /locus_tag="ON516_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3344705.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="ON516_001213"
     CDS             15241..15897
                     /locus_tag="ON516_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3344706.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="ON516_001214"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="ON516_001214"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3344707.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="ON516_001215"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="ON516_001215"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3344708.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="ON516_001216"
     CDS             18239..18757
                     /locus_tag="ON516_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3344709.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="ON516_001217"
     CDS             18864..19364
                     /locus_tag="ON516_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3344710.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="ON516_001218"
     CDS             complement(19644..20564)
                     /locus_tag="ON516_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3344711.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="ON516_001219"
     CDS             20729..21742
                     /locus_tag="ON516_001219"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3344712.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="ON516_001220"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="ON516_001220"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3344713.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="ON516_001221"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="ON516_001221"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3344714.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="ON516_001222"
     CDS             complement(23382..24143)
                     /locus_tag="ON516_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3344715.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="ON516_001223"
     CDS             24332..25426
                     /locus_tag="ON516_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3344716.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25512..>26152)
                     /locus_tag="ON516_001224"
     CDS             complement(25512..>26152)
                     /locus_tag="ON516_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCR3344717.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8644 a         4531 c         5508 g         7469 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
    25501 gttatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt
    25561 tcgattggtg ttctgtaatt caatgatttt cttggaatat gattacgttg attgctgaca
    25621 ctggaaataa atgtctgatt cacttttcta aaatccattg atttcggcag tccattacga
    25681 cgcagaatcc cgttagaatt ctcgtttaat gggcgttgag aaggtgttcc agggtccgca
    25741 aaatatatat caatatcatg ttggttacta atggctttcc agttagaaaa ttcttttcca
    25801 cagtcaaacg taatagattt aaagaagttt ttagggaagc gagaaaacca ttgattaagg
    25861 gcagtttcaa tatctaatgc cttacggccg ttgggtttaa tcgtgataat gactttagat
    25921 aatctttcaa ctaaagtaat gacggcactt ttatgatgaa tgccaacgat cgtatcacct
    25981 tcaaggtgac caaactcaga attgaaatca ggataattat caggtcgatc atggattgag
    26041 cgctgatact gttgttttcc ccgtttttcc tgatggccat tgggttttct tttacctttc
    26101 atcggtagtg tgtcaatatc aaatattcct ttagaaaata aacgataaag tg
//
LOCUS       DAJSNV010000030        25507 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSNV010000030 DAJSNV010000000
VERSION     DAJSNV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.031"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            111..2171
                     /locus_tag="ON516_001225"
     CDS             111..2171
                     /locus_tag="ON516_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3344718.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2254..2823
                     /locus_tag="ON516_001226"
     CDS             2254..2823
                     /locus_tag="ON516_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3344719.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2998..3804)
                     /locus_tag="ON516_001227"
     CDS             complement(2998..3804)
                     /locus_tag="ON516_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344720.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3942..5456
                     /locus_tag="ON516_001228"
     CDS             3942..5456
                     /locus_tag="ON516_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3344721.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5859..6842)
                     /locus_tag="ON516_001229"
     CDS             complement(5859..6842)
                     /locus_tag="ON516_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3344722.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6847..7494)
                     /locus_tag="ON516_001230"
     CDS             complement(6847..7494)
                     /locus_tag="ON516_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3344723.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7579..8211)
                     /locus_tag="ON516_001231"
     CDS             complement(7579..8211)
                     /locus_tag="ON516_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3344724.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8227..8778)
                     /gene="coaC"
                     /locus_tag="ON516_001232"
     CDS             complement(8227..8778)
                     /gene="coaC"
                     /locus_tag="ON516_001232"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3344725.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8775..9512)
                     /locus_tag="ON516_001233"
     CDS             complement(8775..9512)
                     /locus_tag="ON516_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3344726.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9509..10498)
                     /locus_tag="ON516_001234"
     CDS             complement(9509..10498)
                     /locus_tag="ON516_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3344727.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10756..11298
                     /locus_tag="ON516_001235"
     CDS             10756..11298
                     /locus_tag="ON516_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344728.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11315..12400
                     /locus_tag="ON516_001236"
     CDS             11315..12400
                     /locus_tag="ON516_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344729.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12412..13218
                     /locus_tag="ON516_001237"
     CDS             12412..13218
                     /locus_tag="ON516_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344730.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13219..14046
                     /locus_tag="ON516_001238"
     CDS             13219..14046
                     /locus_tag="ON516_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344731.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14043..15116
                     /locus_tag="ON516_001239"
     CDS             14043..15116
                     /locus_tag="ON516_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3344732.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15544..18240
                     /locus_tag="ON516_001240"
     CDS             15544..18240
                     /locus_tag="ON516_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3344733.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18317..18805
                     /locus_tag="ON516_001241"
     CDS             18317..18805
                     /locus_tag="ON516_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3344734.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18817..19311
                     /locus_tag="ON516_001242"
     CDS             18817..19311
                     /locus_tag="ON516_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3344735.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19332..20141
                     /locus_tag="ON516_001243"
     CDS             19332..20141
                     /locus_tag="ON516_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3344736.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20138..20959
                     /locus_tag="ON516_001244"
     CDS             20138..20959
                     /locus_tag="ON516_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3344737.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21010..23109
                     /locus_tag="ON516_001245"
     CDS             21010..23109
                     /locus_tag="ON516_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3344738.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23106..24224
                     /locus_tag="ON516_001246"
     CDS             23106..24224
                     /locus_tag="ON516_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3344739.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24263..25141
                     /locus_tag="ON516_001247"
     CDS             24263..25141
                     /locus_tag="ON516_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3344740.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25287..25484
                     /locus_tag="ON516_001248"
     CDS             25287..25484
                     /locus_tag="ON516_001248"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3344741.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8049 a         4446 c         5407 g         7605 t
ORIGIN      
        1 aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga aatcatctta
       61 tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa atgtgttcaa
      121 aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa cggtcgtgtt
      181 ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta tctgtaggtg
      241 tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta cgtacctctg
      301 tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat gcaaatggaa
      361 aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca acgataaacc
      421 actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc agtgtgccgc
      481 tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa cagatcactg
      541 atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa gtttcttatg
      601 tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa
      661 aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca caaggagata
      721 tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca ggaacatcta
      781 ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca tctgctgtga
      841 tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta agaacaggag
      901 agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga acttcaggaa
      961 ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga gaatatcccg
     1021 ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca gctgtagttt
     1081 atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat agtcaaacaa
     1141 ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct gatttgcgcc
     1201 tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat gaacaaaatg
     1261 ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga aaaagtcact
     1321 ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag caatcaggag
     1381 gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa ggtcatgatc
     1441 ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca gcaacaaatt
     1501 ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa gtaccattgg
     1561 aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt tatcatgacc
     1621 cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca ttaaacaaac
     1681 agatagatca aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa
     1741 tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa
     1801 gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc
     1861 cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa
     1921 aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga
     1981 agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac
     2041 ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt
     2101 tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg
     2161 actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta
     2221 tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca
     2281 gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt
     2341 tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc
     2401 gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct
     2461 tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta
     2521 gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg
     2581 ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc
     2641 ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg
     2701 agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta
     2761 gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa
     2821 taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca
     2881 tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa
     2941 agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta
     3001 tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg
     3061 actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc
     3121 cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt
     3181 tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac
     3241 agtgtttccg tctttactta actccacact attgatttgc tggatgatcg tatctttttg
     3301 catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac
     3361 tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt
     3421 tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct
     3481 gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc
     3541 taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg
     3601 tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa
     3661 aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat
     3721 gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt
     3781 aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta
     3841 agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa
     3901 aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg
     3961 gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata
     4021 cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac
     4081 aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg
     4141 gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt
     4201 tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt
     4261 cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag
     4321 aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc
     4381 actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga
     4441 tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg
     4501 gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca
     4561 acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg
     4621 ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt
     4681 ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga
     4741 caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa
     4801 ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga
     4861 ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg
     4921 tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat
     4981 cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg
     5041 accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt
     5101 taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa
     5161 aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt
     5221 atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga
     5281 acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag
     5341 tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta
     5401 tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata
     5461 gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg
     5521 attgaacact gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     5581 agccaagtaa aaacagcttc ttatgttttt aacttggttg gacttattac cgcagatgca
     5641 agcttttgaa caccgtttat tcggaaaaag agcgtgggac aaaagtaaag aacacttttg
     5701 tctcacgctc taaacacgta taaacggcgg aagcagaaga aattccttcg gaaataagct
     5761 gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa
     5821 acgcttctgt cccagcctct tttttgatca gcatgatttt attcagttat cttcaaaatg
     5881 atttttccga tgtttttgtt tgcttccata tattcatgcg cttgtttgac tgcgctgaat
     5941 tggaatgtac ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg cccagctttt
     6001 tgcataaaag tttgtgcgag ctctgcttta tactgatcac ttctaggtgt caataatgta
     6061 ccaagcagtt ggacacgttt cccaatcaat acaccaaggt ctatctcagg cacgatggtc
     6121 ccacctagca taccaatcaa gacccaacgt ccatcaacgg ctatagaagc taaattcttt
     6181 tcccagtaag aagcgccgat gaaatccaaa ataacatcga ctccccgacc ttcagtgatt
     6241 tttcgtacct cttcttgaaa gtcttgtttt ttatagttga tcaattcatc tgcaccaagt
     6301 tctttacaaa aggcgagttt ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa
     6361 agctgtctag ctaattggat cgctgctgtt cccacaccac ttgctcctgc atggatcaag
     6421 acagtttcgc cttcttgtaa ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa
     6481 acttctgaga ttccagcagc agaaacatag tctagcgtat cagggagaag gattgcacga
     6541 tcggcaggca tcacagcata ttcggcataa ccacctagat tgactaatcc gtatactttt
     6601 gtcccaggag taaaagtgga gatatcactt ttattctcaa ctacctctcc agctacttcg
     6661 actcccaaaa tagggtaggg aggtttcaaa gagactgatt ctcttttcat aatatctgtt
     6721 cgattgacag ctgctgtatg gactttgatc agtaattcgc cttgttttgg tttaggaaca
     6781 ggcacttcaa tttgttctaa tacatctaca gaaccaggat gcttgattga aatggcacgc
     6841 atggatctat tcctcctttg cccattcttg gaatgctgta ttcaactctt tgctaccttc
     6901 agaaagtact agagtcgtac gagaaatatc cgtttcttcc actagagcgg caagtacagc
     6961 agcaactgta gagcgagtga tcgtttttga tgccttctca ttaggcttag ttaattgaat
     7021 ggaagtaact tcttcatcgt tagtcaatgt gactggttga agaatgacaa aatctaagtt
     7081 agttcgtttc agccattcat ctgcataatg tttagtaata taataatctg tcatggactc
     7141 aggccatttt gctggttcat ctgcataaac agcactgacc atcagatagc gagagacatg
     7201 cgcattttca gctgcgatca ctgttttgat tgcgccatcc aagtctactt gcagaagatt
     7261 ctttccttga gagccggcag caaaaatcaa tacatcgatg cctttgaatg cttcggtcat
     7321 tttttccacg gaccaagtta aatcaaaaga gacatacgaa acattctctt tttttactaa
     7381 tgtttgggaa gcaacatctc tcacccctgc aaaaatttca tgggaacttg gagctaaatc
     7441 ttttatcagg tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt tcatttggat
     7501 catcctttcg acgtcaaatc tttccattca atagtaacac caaaagagaa aaaaatcaga
     7561 gatcggctac gatgactatc agttagtaag agaagggtgc ttgaagtatc ttaacaagac
     7621 attgcatact gctgcagtcg caagacctgc gacgattcct tcaggtatac cacttgtaaa
     7681 aataaccgct agaatagctg aataaacagc atttaaattt gttccgatga ctttggcata
     7741 atcttgctgg aataaaaagt agatcatatt catcactaaa atcgtattgg tcaatgaacc
     7801 ggaaagtcca gctaagaata aagaaacgga atctgctttt tgtttcatta gtttgcgcag
     7861 acctttataa acgaaataag gaaccacccc aatcaagatg cgaggaataa acgcgatcag
     7921 caaggctttc cagcttccat gttctgttcc gatgactggg ataaacggag aaaagacaaa
     7981 agacaaaggt gtttggataa ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc
     8041 aagaaaagca cctagtcttg gtcctaaaac gatcgaagca atgattacag gaatatgcat
     8101 ggttgttgca ttgattggac cgatagggat aaaacctaaa ggcgtaacag ctaataaaat
     8161 caaaatagct aaaaacatcg cagttaatgt aaagtttttt gtatttttca taagtactcc
     8221 ttttttctaa gcatttcttt cattaagagt cagcatgacc tgttcgatga tcgtctgaat
     8281 atcagctaag gcaccttttc caaaatctcc acaagctaaa agagattctc ttggttcaat
     8341 ttcctgatag ccgacagatc tcaatgtttc cagattacgc tgagtgatcg ggttttgata
     8401 catattcgtg ttcatggcag gggcaatcaa ttttggtgtt cccactggaa gcgccattgc
     8461 gactgttgaa agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt
     8521 agcaggcgcg atgagaaaca agtcagcttg ttttgctagt tcgatatgat tgattacctg
     8581 cggatttgat tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt
     8641 taatggcgtg ataaaatgtg tgctgctttt agacataatc acatctacct gggcacctaa
     8701 tttgacgaat tgacttgtaa gatcagcgct tttgtaagca gaaatacttc cagtaacgcc
     8761 tagtaagatc tttttcattt tgattcctcc agtctaattt tctcagtaat caatgcagca
     8821 atctcagatt tactttgagc ttcttccact gttccatctt tagataagag atagccgtgg
     8881 tgtgtctcgc catggatttc cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt
     8941 atcagactat tttttgcgac tgccagaaga gattcctttg gtacatctac taatagtttg
     9001 aaaccaatga tttttgcttt tggctgccat tcacgtagat aagaaatgat ttttggtgtt
     9061 ttcttcaaaa cgagcacaag atgctcggtg ttagaagaga tctttgaaac agtggtatct
     9121 gtctgttcat tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga agtgggtagg
     9181 ttttttgcca gctgttcttc tgaaaaacta aaagcaggag taaaatcact gactgccata
     9241 ctgtggataa tcgcatcgta gtgttctttt ttcgttagtt cttctaattg taaaaataag
     9301 tccagtgcag attcgatcgt ataaatcgtt atattggagt gtcttttcgg tttcagtgct
     9361 tgtttcgtag tcacgtaatc gattgtggta tccggattgg cagctaaata atcagctaat
     9421 gcttgcccta atcgtcctgt cgaatgattt gtaatagcac gtacttgatc gattttttca
     9481 gtcgttcctc cagctgtgat tagaatcttc atttatggtc acctacattt actagtgttc
     9541 ttccttggtg tgttccagca agtagtttag atgctgtttc aggcaattga tccagagtaa
     9601 tctcttggac gaccagttca tctgcaatct gcaaatccgc catctgttgc cataaagaaa
     9661 gacgttgatc tattggtaca ttgacagaat caattccaat caactggatg tttcgcagaa
     9721 taaaaggcaa gacagtcgcg tttagtttga ttcccccagc attgccacat aaagctactg
     9781 caccgttata agagatcaat ggaagaagac tgcttagctg ctcgccacct actgtgtcga
     9841 tgacatagtc gatttgctgt tttcctaaag cttttgttgt ctctggcaga aattcatcag
     9901 gtcgtacgac ttgcaaagca cctagacttt ttagccatgt gacagactct tcttttctgg
     9961 aaaaggcaat gattgactgg aatcctagtt tatgtaacag ggctatcgct gtactgccta
    10021 caccgccaga agcaccagta acgacaactt ttttatcgga ggtcatacct tgacggatta
    10081 aagcgttcac acacaacata gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca
    10141 gcgtcatatt ttttggtaga ggaactaacc agtctcctgg tactttttga tattggctat
    10201 aaccgcctgg atgggtgacg ccaagcccaa aaccagtagc aagtacaaga tcttcttttg
    10261 aaaaacgtgg attgttactt tggacgacag ttccggataa atcaatccct ggagtcatcg
    10321 gatatgttcg tatcacctga ccagactcgc tcattgccaa tgcatcttta taattgacat
    10381 cagaataggc aatttttacc aagacttcat tttcagccaa ctcagtaaat ggcagatcac
    10441 tgattgctgt atggaaaaca ggttccttta ttacctgaaa agatcgaaaa gcgttcattc
    10501 gtgagacact ccttttaaaa ttttctttta tcataccata aagcctactc ttttattctt
    10561 gttcttccgt aaatttagac aaaaattagg tgaaaaataa agaatcaaac aactagaatt
    10621 tattccaaaa gataaagaaa tattcagcaa aaaaaatttt acaaagggtt gcctttttca
    10681 ataaatttcg tatactaata cgagtggtag taaacaggaa gtttactatt agaaaacttc
    10741 gattcggagg aaattatgga aattggtgag aagttaagaa atttgcgcat ccaaaagaat
    10801 ttgacacaag aagaactagg cgaacgcaca gatttgtcaa aagggtatat ttcccagttg
    10861 gaacgagatt taagttcacc atcaatggaa acattctttt ctattcttga agtcttaggt
    10921 gtgactccag aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa
    10981 gaagatcata cggtttattt cgatgaagaa aacgggtatg aattggaatg gctcgtggct
    11041 gattcgaatg aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat
    11101 aagacgtttg aaccttcatt atctgaaacg tttatttttg tattagaagg ggaattagag
    11161 ttaagcttag gagagacggt ctatgtggca aaagcaggac agtcgatcta ttatcaagcg
    11221 acaaaacaac atcaattaag gaatcatctc aaaaaacgaa caaaagtatt gattgttgca
    11281 acgcaatcat atttgtaata tagggggtaa aaacgtgggg aaaccaatca tttcatttga
    11341 tcaagtagtg aaacgctatg atgatgaaac agtattaaaa aaagtaagtt ttgagatcga
    11401 acaaggaaaa ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg
    11461 tattatagct ggattcaccg aagctacaga aggggacatc tattttgaag ggaaacggtt
    11521 gaatgatttg ccagctaata agcgacaagt aaatacagtc ttccaagatt atgcactttt
    11581 tcctcatatg aatgtttttg ataacgttgc ttttggattg aagatcaaga aaatgtccaa
    11641 atcagatatc gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc ctggatacga
    11701 aaaacgggag attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc
    11761 aattgtaaat gaaccgaaag tattgctatt agatgaaccg ttatcggcgc ttgatttgaa
    11821 actacgtaca gatatgcagt atgaactgcg ggatctgcaa caacgattag gaatcacgtt
    11881 tatttttgtt actcatgacc aagaagaagc gttggcaatg agtgatgaga tttttgtcat
    11941 gaataaaggg aaaatcgtcc aaagtggtac acccgtagat atttatgatg agccaatcaa
    12001 tcatttcgtt gctgattttg ttggggagag caatatcgtg gacggtgtaa tgatcgaaga
    12061 taatctagtt gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa
    12121 tgaaccagtt gaagttgttt tacgcccaga ggatttgacg atcacgacac cagataaagg
    12181 aaaactagtc gtaacggtcg atactcagct gtttcgtggt gtccattatg aaatcatctg
    12241 ttatgatgaa cagcaaaatg aatggatggt ccattcaacg aaaaaagcta aagaaggaag
    12301 caaagtagga ttggcctttg agccagaaga tatccatgtc atgcgtttca acgagtctga
    12361 agaagaattc gatgcacgtc ttgacagcta cgaagaatag gggggacaag aatgaaaaca
    12421 atgcgcagga tctattccgt cccttatatt ttgtggctcg gtttattcgt gatcgcgcct
    12481 gtattaatga tcatttatca atcttttttt gatatgaatg ggcaattcac gctgaataat
    12541 tatctatctt attttacgtc ggggacatcg ctttctatga cgatcaattc tgtatggtat
    12601 gcatttttga tcacgttttt cacgctgatc atcagttacc cgactgctta ttttttaaca
    12661 aaactgaagc acaagcaatt atggctgatg ctgattattt tgcctacatg ggtgaatctg
    12721 ctgcttaaag cttacgcatt tattgggatt ttcagtgtgc acggcagcgt gaatcatttc
    12781 tttgatttta tcgggatcgg gccgaaacag atattattca cagacttcag ttttctgttc
    12841 gtggcaacgt atatcgagat tccatttatg attatgccaa ttttcaatgc attggaagag
    12901 atgaatcctt ctttgatcag tgctagtaga gatttaggcg cgaatagctt tgagaccttc
    12961 cgacgagtga tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt
    13021 attccttctc tttctttatt catgctgact cgtctgattg gcggaaatcg ggtgatcact
    13081 ttaggaacgg ctatcgaaca gcatttcttg gttacacaaa actgggggat gggttctaca
    13141 atcggtgtga ttttaatcat tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa
    13201 aaaggaggtc gctcataaat gaaaaaattt aaatggtcgt atctttattt agggatcgtc
    13261 tttttattct tatatgcacc gatcttctat ctgatttttt attccttcaa tgaaggagga
    13321 acgatgaaca gctttacagg atttacttgg gaaaattatg aagctgtttt tgaagataca
    13381 cgtttgatca ttatcgtatt gaatacgttc atgctggctt tcttatctgc attgatcgcc
    13441 acgatcatcg ggacatttgg ggctatggga atctattaca cgaaaaaacg acgtgcacgg
    13501 acagcgttgt tgagcatgaa taacatctta ttagtctctc ctgatgttat tatcggtgct
    13561 agtttcttga ttttctttac actactagga ttcggattag gttttcagag tgtattgctt
    13621 tcccatattg ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa
    13681 atgaacgatt caatggtcga tgcggctaga gaccttggtg ctaataattt ccaagtagtc
    13741 aaaaatatca ttttgcctta tctttctcca ggaatcatag ctggatattt tatggcattt
    13801 acctattctc ttgatgattt tgcggttaca tttttcgtca caggaaatgg ctttacgacg
    13861 ctctcagtag agatctattc tcgtgcaaga caaggaatca gtttagaaat caatgcgcta
    13921 agtgcgttag ttttcttgtt ctccatgata ttggtcattg gatactactt tattagtaaa
    13981 gaaaaccgtc caaaacggct gaagaaaaac agaagagtac gcgagggggt ggcacggctg
    14041 cgatgaaaaa attacaatct ctgtttttag gtattctcat cattattgcg gtgctgctgc
    14101 ttggtgttcg tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta cttaccatct
    14161 ataactgggg agattatatc gatcctagtt tgatcacgaa atttgaaaaa gaatatggat
    14221 acaaagtgaa ttatgaaaca tttgattcta atgaagcgat gttcacaaaa atccagcaag
    14281 gtgggacaaa ctacgacatc gctataccaa gtgaatatat gatccaaaaa atgatcaaag
    14341 aaaaactagt acttccatta gatcattcga agatcaaagg attaaataat attgatccac
    14401 gatttttaga cttggatttt gatccagata atcagtattc gattccatat ttttggggaa
    14461 ctttagggat cgtatataac gataaattct ttgatgcaaa tcaaattaag cattgggatg
    14521 atttgtggaa gccggaatta aaagacagtt tgatgctgat tgacggtgca cgagaagtta
    14581 tggggctttc tttgaatagt ttaggctatt ctttgaatag taagaatatg gcacagctga
    14641 cagaagcagc aaataaattg aatcatttaa caccaaatgt caaagcaatc gtggcagatg
    14701 agatcaaaat gtatatgatc aatgaagaag cctccgtggc agtgacattt tctggcgaag
    14761 ctgccgatat gatgtgggaa aatgaacatt tacattatgt gatcccacca gaaggatcca
    14821 atctttggtt tgataatatc gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg
    14881 atttcatcaa ctttatgcta gaaccggaaa atgcggcaca aaatgcagag tatattggtt
    14941 attctacacc aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg tcggacgaac
    15001 aattctatcc atctgatgac acgatctccc atcttgaagt ctacaaagat ctaggatcga
    15061 aatacctagg catctataat gacctattct tagaattcaa gatgtatagg agataatggc
    15121 aaaactcagt aagaatgggg acttatcaac gttctaaaga gctaaaaatc gtcctttttc
    15181 tatctgagtt ttccaatctt ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt
    15241 ttattttttc taccacgttt ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg
    15301 gcccgagacg ggctagtaaa tctaagattg aaaaagttac agaaggataa agtaattgga
    15361 aactatgcaa atatacttaa cccttctgta atctattaat aaaactaata tatgaatcca
    15421 gttcaatatt tgtagctatg aaaaaacata aaaatcatat tagataaaaa ctaaattggt
    15481 agataaatta ttttgtcaga tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa
    15541 aaattggaat acaaatatag aatagacata ttgcagccga tagtttatta tgcttttaga
    15601 cacattaaac tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt tgatgagctg
    15661 atacgactaa cagggaatat gtcttctgtc actgccgaga aaatttcgaa gaatctaggt
    15721 atctctcgtc agaatgccag ccattatttg acgagattag tggaagataa aaaggtcgag
    15781 aaattaccgg gaaagcctgt tttgtggaag cctttagatg aatatgcggt tgtagataat
    15841 acggagcaaa taaacgaagc ctttcattct gtagttggtc atgacggaag tctgagagag
    15901 gtcattcaaa aatgtatagc cgctgtcaaa tatccgccta acggactcag tgtattaata
    15961 aatggagcca cgggtgttgg aaaaagtttt ttagccacaa aaatttttga atacgctatc
    16021 catgagcaaa ttattgaaaa agatgcacca tttgctatct taaactgtgc cgattatgcg
    16081 gacaatcccg aactgctttc ggcaacactg tttggataca agaagggggc ctttacgggg
    16141 gctgaaaagg ataccgatgg tttattggca acggccaaca acggctatct ttttctcgat
    16201 gaggtccatc gcctatcgaa agaaaatcaa gaaaagctct ttctatttat tgatactgga
    16261 aactatcgtc cagtcggtga aaatgtaaat tggcattctg ctaaggttcg ttttattttc
    16321 gccacgacgg aaaaaggtga aaattattta ttagacacct ttgatcggcg tattcaaatt
    16381 agtgttatgt tgccaacttt tgaagatcgc cccattcgag aacgattaga gctgattcaa
    16441 ttattttttc aaaatgaagc ggatgtttta caaaaagata ttattgtttc aagagaagcc
    16501 ttgtctttaa tgttatctca tccctttagt gggaatattg gtaagctgaa gaatatcatt
    16561 aaaatcagtt gtgcggatgt gtatagtcga acacaagaag aaccattaac gattgggaaa
    16621 aatgagatct tgattcagtt aaatatgctg gaatccgaag tagaaagctt gccgattgct
    16681 agtttattga taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc gtttgattat
    16741 gattcgattt tggaaaaaat tactcagctg ataaatgaag gtaatttcaa tgaaaatttt
    16801 tcttcgataa agaatgaagt acagcatttg gtgcatcaca ttgataagga ttctttttta
    16861 tcaatgagta gaaaattgat gattcagatg tttagaaagg tctgggtatc cgttttagga
    16921 aaaacgtacg gattgacgac gagtggacct gttgcagaaa ttattagcag atcctatgtc
    16981 atttccccaa gctataagac agacttaacc gggataaatc gtacattagc tgcaaatcta
    17041 cctcggacga cttacttatg taatcagttc ttggagcatt taccaccgct ctcagaaaag
    17101 gacgagaact ttctaagact gcttattgtc gtgactttat ctgattatgt agatgaatcc
    17161 atcgagctga agggcctgtt gcttgctcac ggagatagta cggctagcag cattcaagca
    17221 gtagtgaatc aattatgcgg caattatgtt tttgaagcgt tggatatgcc agtagagagt
    17281 aatgtgtcag agattgtcga atcgacaagg aatttcgtga aacggcaagt gaatacagat
    17341 agtctagtgc tgatcgtaga tatgggctct ttaaatcaaa tttacacaca gatcaagaac
    17401 gatttaaaag gggagttact tcttctgaat aatttgacga cttccattgc acttgatatt
    17461 ggcttgaaga tgacgatgaa tgcgccattt aaacagattg cggaggatgc agcagacaag
    17521 tattcaatca atgttcaata ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc
    17581 tgtatgtcgg gtttggggat ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg
    17641 gatgaatcca tcgaagtttt cacgaaggac tacagagaac tacgtgaatt aatcgaccaa
    17701 aatgatgaaa cttattttga caagacaaaa ttggtcatca cgacttcgga tcttccacgt
    17761 tccttttcaa ttcctaatat taatgtttat gacatcttag acggtgatgg agcagccaat
    17821 ttggagcata ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt gattcaggag
    17881 ctagtaaaat tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct gaacccaacg
    17941 atcgtgatga gtgaagtgga aacagtcatt tctaaatatg aaaattacta tcatttcact
    18001 ttaaatggcc gtgtaaaact aaatctctat atgcatacgg cattgatgat ggaacgttta
    18061 ttcttggctc ggacagaaaa atctgaagaa ccgaattatg atttgaaacc agatgaagat
    18121 gaattttatt atgtttctag aagtattttt caatcaatgg agatgaaata taattttaaa
    18181 gtcaatgctt atgagctttc gctgctctat gaattattag gaccgtatat tcaaaaataa
    18241 agtgtctaaa aaagtgtcta atctgttttt tttaggttag acactttttt taatgtgaat
    18301 taacgtgatt aaaccaatgt ttttaaaagt tggcacagga attgcttata taaaagtgaa
    18361 cagaaagaaa ggaattaacc atatgaagaa aattctaata gcgactcacg gtcacttagc
    18421 ttcaggcttt cttagcagta ttcaattact gacggggaag gttcaagaga ttacggtgat
    18481 taatgcttat gtggatgagt gtgattttga acaggagttg aatgtatttg taaaccaggt
    18541 gaacgagcag gatcagattt ttgtttttac cgatttattt ggcggaagtg tcaatcagaa
    18601 gattaccaaa acctttttgg agaaagagat tgctgtaact attttgtctg gctttaatct
    18661 gccaattctg ctcgaaatag tattggcaac caaggaatta acaaaggagg atattcagga
    18721 actggtaaat aaatgccaac aggaattaaa gatgaccatt cttgatgagg tacatgtagt
    18781 aaatgaagag gaagattttt tttaggagga agaaaaatga ttaaagcagt gcgaattgat
    18841 gaacgattaa ttcatggaca agtagcgatg acttggacaa agagtctaaa tttaaccggg
    18901 ttagtagtag caagcgatga ggcggctacc aacgatattc aaaagatgac attgaagatg
    18961 gctgcaccat cgaatgtcaa agtcattatt aaaacagttg atgacgcgat tcatttgtta
    19021 aaagatccgc gggcgaagga catgcgcttg atggttttag tccctaatgt caaagatgcg
    19081 gtaaaagttg cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg gaatgctggg
    19141 aaaatgacac cgccatcgga agataagaaa accttatcaa aggaagtgat gttaacgacg
    19201 tcagaaattc aagacttaaa agaattagtt gaactttatc cagataccgt ttttcagcct
    19261 acaccggcga tggaaaaacg ttcagctatc tctattttga gtgattttta gaaaaaggaa
    19321 ggagaaaaat tatgtatgat gcatttgtag tcgctttggc cgtttttatt ggagttgctg
    19381 gacatgaaat gttcgggatg tccatgctga gtcgtccgat tgttgttgct ccgttagcag
    19441 gagcgttgat gggggatttg cagacggggt tgattgtcgg ggcgtcacta gaagccatct
    19501 ttatgggtgt cgtcaatatc ggaatctcta gtacggcgga gccttctttg gctgctgggc
    19561 tggctacagc gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt cccattgtct
    19621 ttccactagc agtattagga ttacagttaa tgaatatgat tttttctttt gtatgtggac
    19681 cgatggctgc taaatttttc aaatttgcaa aatctggtga tcaaaaagga atcatcaatc
    19741 tgcacttctt tatgtggttt gtccactatg cgctttatgc tcttattcca ttctttgccg
    19801 ttttatttgg agcagatgtt gtcaaaagcg tcttggataa tattccagag gtcatcatga
    19861 acggattaac agtcgctgga aatctgctgc cggcagttgg gatggcgatg ctgttacgga
    19921 tgctttggag taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc
    19981 tcgagttacc attgattgca attgccgcag ttggaacgat tattgcaatt gttatagccc
    20041 aaagagatct acaactaaaa aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa
    20101 atgcaacgct tgaccaagaa gaggaggatt tctttcaatg aaattagctg atggaatttc
    20161 taaagatgag aaaaaattgg ttcggaagat gttttggcgc tcgatgacta tgtatgcttc
    20221 ggtgaatccg gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc ccttcattaa
    20281 tcatttctac aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata cacagtattt
    20341 ttcgacgacc attccttgcg cgtcctttgt catggggatt gcggcatcga tggagaagga
    20401 aaattctgaa aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat
    20461 gggaccattg gctggaattg gcgattcatt gttctgggga gtttggcgtg tcgttaccgc
    20521 gggactagcg atcaacttag ccaatgccgg aaatattttg gcaccaatta tctttttagt
    20581 tatgttcaat atacctaatt acttgatgcg atactatggc ggcgttttag gctattcatt
    20641 aggctctaaa tatattgaaa agctgtatgc gaacgggtta attgaaattc tgacaaaagc
    20701 tgctagtatc ttgggtctga tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa
    20761 taccgttttg agcttttcaa tgaagggcga agaactaatg aatgtccaag aaatcctcga
    20821 tagtattttt aaaggaattg taccgttatt ggtaacattg agctgtttca aattactaga
    20881 taaaaaagtc ggcgtcatca aaattatttt tggaatggtc gtcttgggaa ttttattgtc
    20941 attacttgga atcgcatagt aggaagctgc agctactagg ctgcagtttt tttagcaagg
    21001 agggaaaaga tgactattaa tgaagtaatt tattcaccca aacacccatt ttttaatgct
    21061 gagttaaacg acggaaatgt gactacttca aacgaataca gctactatcg gaatattcct
    21121 gatgtaaagg gattgacctt cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt
    21181 tgcgggacga ttcaaacgtt gaccttttat cgagaaaatt tactaactga ggaaaaacca
    21241 ggtgtttggg tcaataaaca gctctctcaa accaattcgt tatccttaaa ggttgaagta
    21301 aacgggattg tctaccattt gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa
    21361 gactgtttgc ctagatatat tcatcattat tcgacgttta aggtcattgt agtatccttt
    21421 gctcccatac tggctaataa gcgtttaagt atgcttgttc aacaggttta cattgtgaat
    21481 gaaacacacg aaagccttca agtttcagtg aaagatgtgc cttcgtatca agagaagtat
    21541 tcggatcagc aaaatgtgtt gatttctcaa aaaggaaata aggacacgat cgcacaacag
    21601 gaagtggcaa gcttcgccat tgcttttgtg gacccaaatg cttttgaaga agtaatgact
    21661 tttcaggaat ctgatatcga aaaatggctg aaggaaacat tgaactattt tgctcagcta
    21721 tttgggcagc tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg tgcactttat
    21781 caaagctttt ctagtttcgg tatgaatcga cagggacagg tcgtcggcag caattggggc
    21841 agctatcctg ctactaatcg aatttggaat aaggatatgt attacgcatc attgccattc
    21901 accatgtttg aaccggagtt atgtcaaaaa acggttctat ggtttgatca atatggcgtt
    21961 aagtttcctg gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc
    22021 agtgcgttac tggctagtct ttattttgaa catactgggg atacaaaatt ctttgagcgg
    22081 tgtccgcagg ttctactgaa tgcttcaaga attattgagg acatattagc tcaacgtcgg
    22141 tcaggggaac caatgctttt tccatctgtt tggttatcag acgcctttgc acttggaaag
    22201 tatcataccg gcagcaatct ttgtctttgg aaggcctgta ttggacttgc agaaattttt
    22261 gaagccctag aacaactgag tcttagcaag cgatacaaaa acattgcctg taaactaaag
    22321 gaatcaattt gtaaacaaat gactatcgat ggtccttttg gtgagcaatt tttggaggga
    22381 atcggtgatg aggagaaaac aacctattca gtgaagcact atcaaaaacc gattcttgag
    22441 caagggttaa tctttttaag tgatgtgatt gtggatggaa aaattaattt attgatgcat
    22501 gatggagaag aatcagacac tacgttgatg cctttttatc aatttttaga taataaagat
    22561 catttatatc agaacaccat ggccttttct gctagttctt tcaatccaac ctatagcgag
    22621 gaaatcaaag gcatcacttg gggattagaa tccggcgcca cttttcctgg ctttatcact
    22681 gttttaatga gcgagctgca caaccctcaa gctttcgtaa cccgattaga agaactaatt
    22741 tgtttggcag atttagacgg ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa
    22801 ggagaagtcg tgcgagactt tggctgcggc aaatgcggct gggcatcggg tctttttgtc
    22861 agcctaatga tcagtcaata tttcggattg aaattcaaaa aaggagtatt gcaaattgat
    22921 cctgttgata atttaacgtt ttcttggaag aatttgaagt tcggtcaagc cttcatttcg
    22981 attgaatgta cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc attgaaaata
    23041 agtgatacga aaaaaattct tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga
    23101 aaaagatgag tatactaaaa aatattaccc aatttaaaaa aatccagagt cgagcaatca
    23161 ctcccgaaaa tccaacaggc gagaaaggta aagctgcgat gttgccaagc gagctaggac
    23221 caagccgcaa aggtcgtggc gcaattccgc tgacttctgg tgaagaaact gtgattgcgg
    23281 atattactgg aacgggaata attcgtcata tgtggatgac gattcgtgat cgaacagaat
    23341 ttggcagttt tgttttacgt gatttaatct taagaattta ttgggatggg tcggatagcc
    23401 ctgctgttga aagtccttta ggggatttct tttgtaatgg gtttggagag cgttgcgagg
    23461 tgaactcgtt gccgatagtg gttaatccta cagggggctt taattcctat ttcgaaatgc
    23521 ctttccaaac atccgctaaa attacgatcg agaatttaca tccgaaggat atgtcctcat
    23581 ttttttatac gataaattac gctttggtgg acgaattgga agaaaatagc ctatattttc
    23641 acgcaaattg gcatcgggaa cgcattacca aattacagga ggattataag ttaattgatc
    23701 gattaaaggg tcaaggctat tacgtgggta cttaccttgc gcttacctgc ctagaacgtt
    23761 attggtgggg cgaaggcgag tttaaattct acattgatgg ggatagtgag tatccgaccg
    23821 taacctcaac aggttcagaa gattattttg gcggggcgtg ggcctttcat gagtacgatg
    23881 agttcggacg tccacatgcg aaaaactatt caacagcctt tttagggtat ccttaccaat
    23941 caaatagaga tgccacaaga gatcgttttg aaacgggaaa attgaatgcc gttcatgcct
    24001 ttggtgacga cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa gaccccatct
    24061 gttttaccga agatttatcg gtcactttac aacaaatcgg caacgatgat gttcgcttat
    24121 ttgagcggca agacgatgtt gctagtgtcg cttactggta ttcaacagaa gcttcaggaa
    24181 atactaaacg tcatttcact gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca
    24241 tattcgaagg gaggaaccta tgatgatata tggggcaatt gaagctggag ggactaaatt
    24301 tgtctgtgca gtcggtactg aggaattaac gattttggaa agagtcagtt ttcccacaac
    24361 aactccagtt gaaacaatga agcaggtcat tgattttttt aaacctttta aggaacaatt
    24421 ggcaggcatc ggggtcggct gctttggtcc aattgatatt caatgcaaat caacgagttt
    24481 cggtcatatc acatcaacac caaaacttgc ttggcaaaat ttcaattttg taggaacgct
    24541 aaagcaatat ttcaatattc cgattgaatg gacaacagat gtcaatgcgg cttgctatgg
    24601 tgaatatgtt gctggttctg gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac
    24661 aggtatcgga ggtggtgcct tgttcaatgg gactttcgtg gaagggttca gtcatccgga
    24721 aatggggcat atgttgatca agcgacatcc aaaggacaat ttttcaggaa actgtccctt
    24781 ccaccaagat tgtttagagg ggatggcagc aggaccagcg attgaaaaac ggttgggtgt
    24841 aaaaggccag aatttattgg ctgacgattc gttttggcag attgaagcct tttaccttgc
    24901 ccaatgtgca tacaatacaa cactgatgtt ttcgccagat agaattattt ttggcggtgg
    24961 tgtaatgaaa caagaacaca tgaagaaaaa agttcaggat aaatttgttg agcttataaa
    25021 tggatatgtt gaaataccac caattgatag ctacattatt acgcccgaat taggtgataa
    25081 tgccggaata atcggcggat tagcattggc cagaaaagcg gtaagaaata agcaaccata
    25141 attaatgaat gtttcattga tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc
    25201 aaatgaagtt ggataaagcg aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt
    25261 tgttttcatt aaatcactga aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg
    25321 cgctgtatac cttgaggata ttcactggtg gcgtacactt aaatagtaat atattgtgtt
    25381 actttatgtc tgtggtaata gttatagttg cttaccaatg tatgttcatt tttcgaaatg
    25441 ttgacttcat aatccttggc ttaatgtttg aatttttttg gtgatagatt ttttcagccg
    25501 tataaaa
//
LOCUS       DAJSNV010000031        25115 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSNV010000031 DAJSNV010000000
VERSION     DAJSNV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.032"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..3313
                     /locus_tag="ON516_001249"
     CDS             35..3313
                     /locus_tag="ON516_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3344742.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3854..5761)
                     /locus_tag="ON516_001250"
     CDS             complement(3854..5761)
                     /locus_tag="ON516_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3344743.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5891..6766
                     /locus_tag="ON516_001251"
     CDS             5891..6766
                     /locus_tag="ON516_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3344744.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6846..7820
                     /locus_tag="ON516_001252"
     CDS             6846..7820
                     /locus_tag="ON516_001252"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3344745.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8000..9145
                     /locus_tag="ON516_001253"
     CDS             8000..9145
                     /locus_tag="ON516_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3344746.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9282..9626
                     /locus_tag="ON516_001254"
     CDS             9282..9626
                     /locus_tag="ON516_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3344747.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9897..11183
                     /gene="efmA"
                     /locus_tag="ON516_001255"
     CDS             9897..11183
                     /gene="efmA"
                     /locus_tag="ON516_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3344748.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11321..11920)
                     /locus_tag="ON516_001256"
     CDS             complement(11321..11920)
                     /locus_tag="ON516_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3344749.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12324..13445
                     /gene="mnmA"
                     /locus_tag="ON516_001257"
     CDS             12324..13445
                     /gene="mnmA"
                     /locus_tag="ON516_001257"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3344750.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13691..15112
                     /gene="arcD"
                     /locus_tag="ON516_001258"
     CDS             13691..15112
                     /gene="arcD"
                     /locus_tag="ON516_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3344751.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15126..16244
                     /locus_tag="ON516_001259"
     CDS             15126..16244
                     /locus_tag="ON516_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3344752.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16304..17596)
                     /locus_tag="ON516_001260"
     CDS             complement(16304..17596)
                     /locus_tag="ON516_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3344753.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17728..18306
                     /locus_tag="ON516_001261"
     CDS             17728..18306
                     /locus_tag="ON516_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3344754.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18435..18812
                     /locus_tag="ON516_001262"
     CDS             18435..18812
                     /locus_tag="ON516_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3344755.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18745..20817
                     /locus_tag="ON516_001263"
     CDS             18745..20817
                     /locus_tag="ON516_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3344756.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21028..21975)
                     /locus_tag="ON516_001264"
     CDS             complement(21028..21975)
                     /locus_tag="ON516_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3344757.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22221..22685
                     /locus_tag="ON516_001265"
     CDS             22221..22685
                     /locus_tag="ON516_001265"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3344758.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22747..23790)
                     /locus_tag="ON516_001266"
     CDS             complement(22747..23790)
                     /locus_tag="ON516_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3344759.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24167..>25115)
                     /locus_tag="ON516_001267"
     CDS             complement(24167..>25115)
                     /locus_tag="ON516_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3344760.1"
                     /translation="IIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIFIF
                     VKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQ
                     VILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISLIC
                     YAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFAL
                     SQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFL
                     KVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         8212 a         4601 c         5281 g         7021 t
ORIGIN      
        1 aaacgctttc taatttgtta cgggaggaat acacttgtta aaaaaacgta taaaatacag
       61 tgcgctaatg atgaatctcc ttatgctcag taccccggtt ttaatttctg tcgttgaagt
      121 tgctgctaac gaacaacagt ctgtgaacga agaaaatgca cacgtgaaca ttgaagggat
      181 gtcaacaatg gaggaaatac caagaactag tgaagatatg gatacggaag aaggaaatga
      241 tcctatgatg aaagaatcag aaaatgaaaa gacaaaaaag caatctgaag aagcaaatga
      301 agagtggaaa ggggttgttt tcggagaaag cacaagtgct agtaggtatg ccattgaacc
      361 attagaagac ggcgtgcggc tctcttccac gaataacggc ggaaaattcc agtcaagcgg
      421 atcagatgga atgacctatt attatcaagc aatccccaaa gacatcaact ttaggatgcg
      481 ggcgacgatc gaaatagaat cttggaccta tacgaatgcc caagaaggat ttgctttaat
      541 ggtaagagac gcagtaccaa aaaatcattt gtttgggcag gcattttatt caaattcatt
      601 tgccatacta ggcagtcgga tcgaatatgt ctgggactcg gatagacaag aagtggtcaa
      661 cacgagcggc agcaaatata cgatgcgact tggattgggg acacgggcaa tcacaggaat
      721 atcatctgaa gatccgcaag ctgcgccagc tcctgggagt gtctcagtgg aaacaacacc
      781 tttagagact tcagcagggt tcttggaaaa agaaacaggt tcttataata tattcggaaa
      841 cggtcatggg aatctgtttc cggccacgaa ctcgattact aaactggacg tagagatgaa
      901 aaaaacgaat actggtttag aggcatatta ttacgatcct gaaacaggag aagaaatggc
      961 ttctgacatc atgtatgact gggaaaaatt atacgcttca gatgaatcgg ttatttatgc
     1021 aggatttgct gcagcaagga atatgacgat caaggtagaa ggaatcgaag ttgcttactc
     1081 agatcctgcg acagatcctc ctgggcaaga aagacctact cgcttgattg atccgattta
     1141 cactgtgacc tctagtaatc attcaggaaa ccaagatcat actatgtcct ttagagcaaa
     1201 tgctgacgga ttattgacaa ttaaaaataa agcaacagga gaggtgctta agaacgcaaa
     1261 aaacttggct atcactgcga atcgggaatt tgattatcag acaaaactga tggaaggaac
     1321 gaacgaattt actttcagtt ttacgccaga taaaaactat ccgccagaag aatacgtaga
     1381 gatgactagt tatgaaacaa aagagattct tcatacagtg gactaccgta aacctaaata
     1441 ttcgacagtt tatgtaacac cagaagggtc agatgcagga aacggaagca gacaaaatcc
     1501 attatctctg acacaagcat tgagatacgc atatgctggg cagacaatcg tcatggcgcc
     1561 tggaacttat tccttcgaaa gaggattgac gattccaaaa ggcgttaacg ggacaagaga
     1621 aaacccgatt cagttgattg cagagaaaaa tccagaaaac aagcgtcctg tacttgattt
     1681 taaaggaact ggaaatggga tgacactgtt cagtcactac tggggtttac gagggtttga
     1741 cattacaaat agtgcagcta tgcaaaaagg attacagatt tctggaaatc ataatttgat
     1801 cgaagagatt catgcttatc ggaatggaaa tacaggaatc caaatcagcg gtagctcaaa
     1861 tgatcctttc gaagcatggc ccgcgcataa cttagtcaag aactgtacat cttttgaaaa
     1921 tgccgatcca ggatttgaag atgccgatgg atttgcggcc aaactgacaa taggagaagg
     1981 aaatgtattt gacggttgta tcgcgtatca taatgcagat gacggttggg acctttttgc
     2041 taaaaatgaa acaggttcga tcggaaaagt aatcatcaaa aactcagttg cttatcgaaa
     2101 cggctgggta ccaggaattg agggagaagg taacggtaat ggattcaaaa tgggcggttc
     2161 ttctttaacg ggccctcatg aattgatcaa tagtttgtct ttcgaaaatc ttgcgaaagg
     2221 aatcgattcc aatagcggaa cagatattat cgtcaatagc agtacaagtt ttaataacgg
     2281 ttcatataat gtggctcttt atacctcttc agcaggaaac acagactacc atgcttctgg
     2341 tatcttatct ttccgaacag agaatcttga gatgagagag cagatccgcc cattgaatca
     2401 aaacgaagat caaatctacc actcgttgaa ctactattgg aatgaacgag agaaacaatc
     2461 agaaaatact cttggacata ccatatcaaa agattggatc gagtctctaa atacatgttc
     2521 gaaagaaggt cagcagcctg ttacgcgcta tgaaaatgga agtatccatg ttcatggact
     2581 gatgcagatt aaaccgggga atcgtcaaac agaaaaggaa agagtggatg gtcttccatt
     2641 atctgtaggt gctatttttg acggagaaac ctcaccgtct tcggaatatc cagagtatgc
     2701 aatcgtacaa gaaccaaatc agacaaaacc aaatgaacaa aataatccga ttattataca
     2761 agaagcaaat ggtgtggcgg agaatgcaga aggaaatatc attgaactga agccttttac
     2821 agtttttgtg tcaactttca caccaaatca aggaacgcct gtctttgaag atatgcgtta
     2881 tttcccagaa aagatccaaa tcgttgaaac acagtcaagt gctcaatttt ctaatcatac
     2941 cgctattttt tcagcacctt caaaaggaga atatacgcta gaagtcactt atcgttcgga
     3001 aatatttgat ggggaaaaat ggtctgctca tacagaatct gttacccagc aaaaagcgat
     3061 cactgtgcga gaaactgccg atgtgccccc gggaaatgac aaaccagaaa gagaaaatcc
     3121 aggaaatgaa tcaccaaatg gaaaaccaaa tggaaaacct tctcatccgg ataggacagc
     3181 aaaagaaagt gaaaaaaatg gcactcaagg actcccaaaa acgggagaat ttaataaccc
     3241 gttgctagca ttagctggag gaattttgtt aatcggtgta gttgtttatg tcatgaaaca
     3301 gcgaaaaaaa taagtggaat ttctcctttt atgatgtaaa acaattgact gaggaaagta
     3361 aaaatagaag gaaatgagac caatagatgt ctcacttcct tctatctttt tatagatgag
     3421 gttctccttg ctgcttttga ttggctgttc ttattacttg atgcaggaca gctttttcac
     3481 aatctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacggca
     3541 aaatatgagg aactattttt ccgttttttt ctctaatact caggtcagga cgctgatttc
     3601 acaacctctc gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc
     3661 aaaaatgtgg aaagccattt tcacaaaatt tttcttacta cttgaaacta agcagctttt
     3721 tcacaacctc taatacatgg tattccagaa gtaacttctt cgaaaaataa gccgaaatgt
     3781 cacaaaaatt tgagaagcga ttttcccaaa ttccttctta tttcttgaag ctgaccactt
     3841 ctggcacaac ctcttaccag attcgcactc ttttttctgg cgctagatac atagcgtctt
     3901 gttccgtgat accaaatgtt tcatagaatt cgtcaaggtt ttttggttgc acgtttgctc
     3961 ggagtttagc aggggcatgg acatcgattt gcagtaaaag ttgttggtat tctttttttg
     4021 cttttgtacg ccaaatcgtt gcccaattga caaagaattc ctcgatagaa agatctgatt
     4081 ccgtttttgc tgcttccaat gcacagctta gaccgcctgc atcagcaata ttttccgaaa
     4141 cggttaattt tccgtttact tttccgtcag caaaaggcag accatcgaat tcttcgatca
     4201 ttgcttgtgc tttgttttga aattgtgtaa gatcttcttc tgtccaccag ttgttcaagt
     4261 tgccatattc gtcaaataaa gcgccgttat tatcaaatgc atgggaaatc tcatgagcga
     4321 taacagctcc gattccacca taattagcag agcttgattg ttctagactg taaaatggtg
     4381 cttgaaggat cgctgccgga aagacgatca cattccgaaa tggatgataa taagcattga
     4441 ccgtgtcagc actcatttcc cattctgaac gatccactgg tttgttccat ttgctgaaac
     4501 gatcttttaa tgtgagtttg ctgaaaacaa gagtatttga aagcaaagta ccaccctgat
     4561 ctgcaggtac tgttttgaac tggtcgaata atgctgggat cttatcgggg tagcctactt
     4621 ggatacctaa gttatttaat tttatgacgg ctttttcttt tgttgcatcc ccaagccagt
     4681 cattttcttc tagacgtttt ttataaacgg cgatcatttt tttcaccatt tgttctacgt
     4741 cttttttagc agcttcgcca aaatattttt ttccgtagta atcgccaact acttgatcga
     4801 attgtccaga ggcaaggtaa tacgctgctt ttttctgagg cattgcttcg tctgttccag
     4861 acaatacgcg agaatagatt ccgctgactt ggcggaattc ttctgataga taaccactaa
     4921 gagaacgtac tgttttaatg atcatccagc ttttgaataa cggaaaatga gcttctgtca
     4981 ggatgtcatt caaatgatcg aagtacactg gatcagttac gatcacttta tctggagttg
     5041 tcccaatcaa acccgtgaca agttttttca agtccagttt atctgtgtat ttaataaatg
     5101 tatcaaaaga ttgcggatta tacattttgc tgtaatctgc gttttcttct gcacttttta
     5161 catgaggagc aatcaattta tcaaattgca ctgcttgttc cgtgattgct tctgcctgac
     5221 ttttttcttt gccggcaagc tctaataact gaacagccat atcaaagaaa acgcctatta
     5281 gttgtgcacc gttgggatgg tctgatgcat aatacgtctt atctggaagg aataaagaag
     5341 gcggataagc aaagaaggcg ttcgtttgcg cattcttcat atctgcatct acatccagat
     5401 taaatggcaa tggtaatgta tccaacagcc attctggaaa ttgcttattc aattcgtcga
     5461 aagactgcaa tgattcaact ttttcgatta taggttgaag cggttgagca tgcaactcat
     5521 ttcttcggtc ataatcgttt gccagttgat aatatgcaag gaaatgtttc attttttctg
     5581 aagacacttt ttctggatgg gcaagcagat cgtctgtttc tcccatcaaa agttggtcga
     5641 tcccgtcaac taaatcttgg aaaccgcctg tagctggttt atctgcaggg atttttgctg
     5701 ttttttgcca ttcttcattg actgcttcat agaaatcttg tttcaatcgt tctttgttca
     5761 ttcatcgatc gctccttttt tgtttcgtgt gcccattatc ttacaaaatg aaggaattaa
     5821 caaatagaac ataaaaaatt gaaaattcga acaatcaaac tgatttgttt ttttcaatcc
     5881 ttcacttata atgaaaaaaa ggaggcgatc ccaactgaat caagtagtgg ataaaccttt
     5941 atatttcaaa gtagataaaa aaattaaaaa attagccagc actcagcagc tgcagtcaag
     6001 aaaaagtaaa ttgctttttt tagctctggt ttttgaagat caaagttatg tgattatcga
     6061 tcaatcagga catccggtag aatattctcc agcaaaatat acttatcaag agggtctttc
     6121 ttgtaaaaaa tggaagctga tcaatgaaga gccaatcgag ttatctagat ggatcaacag
     6181 aaaagaagat attcccgttt tgatcgaaga aaaaaggtca ggaaaggaac tcgcaaattg
     6241 ctgggtggga ttgccagaag aacgtttttt gaggtataag aaatgggcta ccccctcagg
     6301 ctatctttgt ggtacttacg cggcagcagt tctccttgcc tattatcaag actatcgtaa
     6361 ggggtggatg cttccaaatg agattcgcaa gaaaaatacg gctgattcgt tggttttgac
     6421 gaaggcattg cgtagccaga tccaaccact aggacttcct acgattcctt ttcaggttag
     6481 tacagggatt tctagttttt taaagaagaa tggcaatcat gagcgtgcac gagcaactct
     6541 tctaggttca tggcagcgag ctacaaaacg tatacgtgaa ggcaaacctg tgatgattgg
     6601 tattttgaaa gtacttggca gtacatatgg caatcattgg gtgacagcgt acgcttattt
     6661 tgaaactgaa acaggagaac gttattacaa agtacacgac aactggggcg attatcataa
     6721 agtcattcct gccagttgga gtaacggaac agtttcactt ccttaagaat ttgctgaaat
     6781 taacagaaaa tcttggaaaa atatgttaaa ctatcgaaga atagagaagt tttggggagg
     6841 actacatgag taataagtat caggatgaca cgttgaagat ttttagtttg aatggcaacc
     6901 gtccattagc tgaaaaaatc gcgaaagtgt tcggaacaga attaggtaaa agcgtcgtga
     6961 agcaatttag cgatggcgag atttccatca atatcgaaga aagtattcga ggggatcatg
     7021 tgtatatcgt tcaatcaacg aatgcacctg tcaatgatta ctatatggaa ctactgatca
     7081 tgatcgatgc aatgaagcga gcaagtgcga aaacgatcaa tgtcgtattg ccttactatg
     7141 ggtatgctag gcaagaccgc acggcgaaac ctcatgaacc gatcactgcg aaattgatcg
     7201 ctaacttgat cgaagaagca ggggcaactc gagtactgac tttagatctg cacacggttc
     7261 aagtacaagg attctttgat attcctgtag ataatttatt tacgatgccg ttgtttgctc
     7321 attattaccg tcaattaggt ctgacgggag atgacatcgt agttgtttcg ccaaaaaaca
     7381 gcggtgttca gcgtgcacgt agtctttcag aatatttgaa cagcacatta gccatcgtag
     7441 atcatgcaga tgatgcttca actgagggtg gttatgtgat cggtgatgtt caaggaaaga
     7501 catgtatcat ggtagatgat atcttgaaca caggagcaac gcttgcacga gcagcaaatg
     7561 tattaaaaga aaacggcgca aaagaagtat atgcctgtgc gtcacatgga ttattgtccg
     7621 atcatgcgaa aagtatttta gacaatgcac caatcaaaga tatttgtatc actgattctg
     7681 ttttcacaga agaaaatcgt catccagaaa acttaacaat cgttacatgt tcagaattaa
     7741 tgggtgaagc ggtcaaacgc atccatgaaa atacaccgat gagtccgtta ttccgtctgg
     7801 aagaaaaaga attcgaataa acaaaaaaaa gagggaaaga agttgtgaca gaagtgagca
     7861 gcttcaagga gctgcgacaa gacttttgtc acagctcctt ttttctatgc ccatgaaacg
     7921 tcctctagac aaaaatgagc tcttctttgg tttgttcgct attttatgct agaatgtgcc
     7981 tagcaataat ttggaaggga tgaatgaagt gaaaactatt tatctggatc atgcggcaac
     8041 tacccccatg catccacaag taattgaagc gatgacggca atcatgcaag acacgtttgg
     8101 aaatccttca agcatacatg gatttggtcg ccacgcgcat gaaaaattgg aaacagcaag
     8161 acaaatcatt gcagacagtc tgcaagcaaa acctcacgag atcatcttta acagtggggg
     8221 aacagaaggg gataatacag ctatcttaga aacagcgttc tcgcgtcaaa aagaaggcag
     8281 acatctaatc actacagcta tcgaacatcc tgctgttctt aataccatga aatatttaga
     8341 gacaaagggg tttgacgtta catatctgcc tgtagatgaa aaagggaatc tgtcgatcga
     8401 agaggtcaaa aaagcgttac gagaagatac gatcttggtc tctgtgatgt acgggaacaa
     8461 tgaaatcgga aatttgatgc caatcaaaga aatcggtgaa cttttagcgg atcatccagc
     8521 ttatttccat acagatgcgg tacaagcgta cggaaaccaa acgatccatc cagaagaatt
     8581 aggaatcgat ttattaagta tctctgctca taaaatcaat gggccaaaag gcgtcggttt
     8641 cttatataaa cgagacggag taaatatccc tgcattgctt ttaggcgggg agcaggaaga
     8701 aaagcggcgt gcaggtacag aaaatttagc agggatctgg ggtatggcaa aagcagttga
     8761 actgctgtca cctgaagaac gtgaagcacg taacaacaaa tataaaggat tccagcaatt
     8821 gattcttgat acattagaag caaacgaaat cccttatcaa gtaaatggtg atttaacgaa
     8881 taaactccct catgtgttga atcttcgctt aattggcgaa gcaaatgatt tgctactgat
     8941 gaagcttgat cttaatgggg tcgctatttc tactggttca gcttgtacag caggaaatat
     9001 cgagccttca cacgtattag aagcaatgta tggagaagat acaccgatac taaaagaatc
     9061 catccgtatc agctttggtt tgggaaatac cgaagacgac atccagtatt tccttgatca
     9121 attagttcgt gctgtcaaaa aataactagt gaagtcattc ttatatgaac agatgtctga
     9181 gagaacgcct tcatttttta gggaacttaa ctgaaaaacc tagtaaaaat caggataaac
     9241 ttttataata aagagaaact aattttatcg aggtgaaaat catggcattt gaaacaacag
     9301 caacggtact gggcgcacca attagctatc gtttgcatcc tgatgcaaaa agatatacat
     9361 tgcgcgataa tggatttaca gaaacaaacg gaggcaatta tcaattgatc cgaccattag
     9421 atgccacgcc gcaaagcaaa gaaggattca aattaaaaat cactgtatca aaagatattc
     9481 aaacattgaa aatgtcgatc actacagcaa atggattgaa agcagtcaat attttcaaag
     9541 atccaaaaca gaaaatgagc caagataaat tttattttct gatggatggg atgattagcc
     9601 gtgggttatt cgaaaaagtc gaatgatctg tggatataaa aagacaacta gttttgaaac
     9661 ggcctattgt tagatgacta actttgtaag gacgacgcaa attagttgtc ttttttattt
     9721 ttaaatttaa cacatgttgt tatttttaat attttgctga tttattgttt ctaagtaaag
     9781 aaatttaaat tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc
     9841 gtataatgat tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg
     9901 aaaatgaaca gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg
     9961 gacagttttt gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga
    10021 caagagaaac cggttcggcg acaattttga gttttgctac tttactagga atgattccca
    10081 tggttttact aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt
    10141 tgattgtcac ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga
    10201 tttcagaatc cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac
    10261 aaacattcca aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca
    10321 taacaaggac gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag
    10381 ctctaggtgc ggctttgttt tcagtcgttc ctgtcaatta ccttatttta ttagatgtat
    10441 tgggggcagt ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg
    10501 aaatattgga agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga
    10561 tggataataa agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca
    10621 tgccagcaat cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac
    10681 aggcgggagc agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca
    10741 gttttatagg aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg
    10801 gcctgacgat cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc
    10861 ttatcttgaa tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa
    10921 tgatccagca gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt
    10981 tgatgaacct ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag
    11041 gggtagaaaa aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat
    11101 ttttaacgtc agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac
    11161 accatgagca aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact
    11221 ccatttctga tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa
    11281 ttatctttat ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc
    11341 tctttgcttc atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt
    11401 tttcctttaa gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag
    11461 gtacgctgta atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca
    11521 acatcggata atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat
    11581 cataaaaata aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac
    11641 ggaaaaaagc gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag
    11701 tgaccagttc caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc
    11761 ctagatgata aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt
    11821 ggttcatttc atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc
    11881 gttcatggaa aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt
    11941 ttcttagagt aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt
    12001 gtaaatgaga cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa
    12061 tttataaaat tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt
    12121 tatatcgaat gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa
    12181 taggtccagt agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat
    12241 atgacgtata atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat
    12301 ctaattgaaa tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga
    12361 tgagcggcgg tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg
    12421 tgatcggaat attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca
    12481 cggaagatta taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg
    12541 tgaattttga aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg
    12601 caggacgtac acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct
    12661 tggattatgc aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca
    12721 ctcgcgatga aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa
    12781 cttacttcct tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg
    12841 gcatggaaaa atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga
    12901 aaaaagattc aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca
    12961 actatctgcc agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac
    13021 atgctggctt gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag
    13081 gagattcgca agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg
    13141 tggggcaagg gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc
    13201 actttacgac gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc
    13261 gtcagcaaga cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat
    13321 tcgacgaacc tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg
    13381 aatgtctggg tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg
    13441 tttaagcgat attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt
    13501 tggatagcat agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat
    13561 acttgtgtaa aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa
    13621 gagcagttgc aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag
    13681 ggtgatggaa atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt
    13741 tgtcagttcg tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc
    13801 agctccaggg ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt
    13861 attatcgctg aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta
    13921 cgcggaagct tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc
    13981 tgcgtggctg ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt
    14041 cccgatattc aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg
    14101 gggtctgact ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt
    14161 gacgatctgt aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa
    14221 agtgaatata ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac
    14281 agggcagcaa ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt
    14341 ctttgtaggg atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc
    14401 ccagcaagca tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat
    14461 cttgccatac ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc
    14521 aaatatttta aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat
    14581 tatttcgatc attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt
    14641 gatggctcga gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc
    14701 aacttattcg ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt
    14761 tactgattat gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta
    14821 tctacttgtc gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt
    14881 agtgatcggt gggattgctg tagtttttga attgatgggc atcattttag caggattttc
    14941 ttatgtcctt ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg
    15001 tcgtgaaacc aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt
    15061 ggccgcactg ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa
    15121 gaaacatgaa aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag
    15181 cggtatatga tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct
    15241 tagatgggac tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg
    15301 gctggatcga aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg
    15361 atccggaaaa catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg
    15421 cgttagtgga agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg
    15481 acatccccaa atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt
    15541 ggcagtttga tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt
    15601 tgaatagtgc aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg
    15661 cagaaattgc tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa
    15721 cggataaagg tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt
    15781 ccctatccaa aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg
    15841 agttggcaga agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt
    15901 cagatgatct tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa
    15961 agatcattac agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg
    16021 aacttgtcgc tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg
    16081 accagacatt ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg
    16141 cctttgattt accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa
    16201 agggattatt cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag
    16261 gacagtctgt gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag
    16321 gagagtgccc tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat
    16381 ggatctcttg tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg
    16441 aaagaaagac gccacgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta
    16501 ctacgttacc agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat
    16561 gaggcgaata ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac
    16621 caatcaaaaa acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat
    16681 aaaccagaat cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac
    16741 ctaagcgacc aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga
    16801 ttccgctgat caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga
    16861 tgattggact aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga
    16921 tgacgacgtg tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt
    16981 ttttcactgg gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata
    17041 agatgatccc tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc
    17101 caagaagcgg tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt
    17161 gaccactttt ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac
    17221 cagaaaaaat cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac
    17281 ccatcaaaga aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc
    17341 ctttttggtc ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag
    17401 atgaaaaagt caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat
    17461 ctatgtaaag tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc
    17521 caaaccataa gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct
    17581 ttattcttgc tgccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt
    17641 ataaactaca atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt
    17701 aaactgaaag agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat
    17761 ctgtctcaag agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag
    17821 caaataacct ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac
    17881 catttcaaaa atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt
    17941 aatgaccagc tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat
    18001 gcagaaactt atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt
    18061 gtaccgcata ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt
    18121 ttgcagatat ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa
    18181 cttcgtagta tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa
    18241 ccaacaatcg atccagaaga cagctatttt tggatgatca ataactttat cgctactttg
    18301 ccttaaacaa tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat
    18361 aagagaaaat tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa
    18421 gaaggagcac gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt
    18481 tttttgaaga ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag
    18541 cacgacaagc gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg
    18601 caaaaagtaa ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt
    18661 gtgacgatga tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca
    18721 cagtagaaag cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt
    18781 gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga
    18841 cccaaggtga tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat
    18901 ctgaataagg aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta
    18961 ggaatcaaac cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag
    19021 cgaaaagatg tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt
    19081 gctcgctgga tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct
    19141 tctcaaacag ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat
    19201 gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat
    19261 gtgaccagag ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca
    19321 ccgacactag cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg
    19381 tatttcacga tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa
    19441 tttgctttaa aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa
    19501 ggaactgtca cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat
    19561 ttaacagaga tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta
    19621 agtcttgtac aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc
    19681 aaatatctga caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac
    19741 tttgctcctg aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta
    19801 ttccaagaca gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt
    19861 tatgaaaaat taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta
    19921 ggactgtttg ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa
    19981 gaacgattca tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa
    20041 actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc
    20101 aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg
    20161 gaaaaataca gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa
    20221 tctgaactga tggaacgtac ccctcaagga ttgcaagtat ctcctaaagg taagcaatta
    20281 ttagtattgg taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac
    20341 cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa
    20401 gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg
    20461 attacccaaa agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac
    20521 ggcaaaatct atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa
    20581 gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac
    20641 ggtgcttatt tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag
    20701 cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca
    20761 gaagaaccag aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa
    20821 gatatgaaag aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa
    20881 aattgcttct caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac
    20941 accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa
    21001 aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac
    21061 tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc
    21121 aatatatttc ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat
    21181 cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat
    21241 gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt
    21301 taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa
    21361 ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt
    21421 aaatccgacc cagctaaata atccttttga aaatcggttg tattctgtta attctaggac
    21481 ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt
    21541 atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg
    21601 ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg
    21661 gttgatcatc tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac
    21721 taaatctcct gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct
    21781 ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa
    21841 cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa
    21901 tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac
    21961 aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca
    22021 aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat
    22081 tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag
    22141 aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct
    22201 attgactagg agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg
    22261 cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga
    22321 atcagcgaat tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa
    22381 aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga
    22441 aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg
    22501 ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa
    22561 tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa
    22621 acgcaataga tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa
    22681 attaaaaaca gttgtgattc ggacattgtt ccatgcgaga atcacaactg ttttttcatg
    22741 ttcattctaa actttgatca gccaaataaa tagtaaagcc atcactgttt ggagaagccc
    22801 gacagctaat ccataagata agaagcgtgt accttctttc atgaattttg caaagtctaa
    22861 acgcagacca attgccgcta aagcagtgat ctcaaaccag ctgctgataa aatgtgccga
    22921 ttggtcaata actacaggta aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc
    22981 taatacatac caaggaagcg gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa
    23041 cagagctggt tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa caacgagcat
    23101 cataatacgt agaatcttaa atagcatggc gaattcgaca acagaattgt caagtaagct
    23161 tgccccagcg actacttgac caacagattg gagtgtacca cccaaaagtg cacttttttc
    23221 aatgagattt ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca tcatcacagt
    23281 acccagtaga ttgactagtg tgatgatctg tcctttttct tcctctttcg catggatgga
    23341 aggggcgatt gaagcaatag cagaagaacc acaaacagcg tttcctccag ccatcatcaa
    23401 agaagaagta tcgctgaatt gcagtttttt accaatcaag taagcaccga tgatagtgac
    23461 tgacatttgc agtaacacaa aaacaattcc ttttacacca atttgagcaa tcgtttgagc
    23521 tgtgacagtt gcacctagta aaaaaacaga gaattctaat agtttgcttt cagcaaattt
    23581 tgtgccgatt gctagaacag gctgtttaaa aaaagtattt cctaataaaa tacccaataa
    23641 aatagcaata gtcgctccac ctaactgtgg cagataaata gcaagaagcc tgctgaaggc
    23701 agcgatggca aaagaaagca gtaaaccggg taagatttcg cggaccgctg aatcctttac
    23761 agattttatt ggatgtgaca atgatttcat ttgatcggtt cctttcgtca ataaatacta
    23821 aaagcatact cgaacatagt agcactaaac cagtattttg acaaaagaac aagaggtcgt
    23881 gacaaaagtc ttgtacgacc tcttgtttcg aataaacggt gttccagaag tagcttctac
    23941 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    24001 tcttgaagct gaccacttct tttacgacct cttggtaaac ggtgttcaaa agcttgcatc
    24061 tgcggtaata agcccaacca gtacaaaaac atgaacagct gtttttgttt ggccgttctt
    24121 attacttgat gcagaacagc tttttcacaa cctattgaat cgattattaa gcgatttttt
    24181 tagaaacaaa ttttccatgt ttgctatgtt ctttaattaa gaaacgtacg taatgagcaa
    24241 gaacgtagaa gacaactaca cttgcaaaca gacattccgc aacttttaag aaatccaata
    24301 cgctactata gatacctaaa ctgttgaagt aatgaacagt agtaaatata gctgttgcag
    24361 agataacaag cgggaatgta aaggctgcat agcttggata aaaacgaagc ttgaacaaac
    24421 gtggaagcat agacagcaca acgaaataca atacttgaga taaagcgaac aatgctacga
    24481 caagataaaa attaggttca gcaaagtttt taagataacc tgttaggcaa agagaaccag
    24541 gtgctgcaac gatcgtaatc agcggacgtg tcggatgagc catatttctt acgaaaaaga
    24601 cacgtttgat gatgatcggc aataagaacg catagcagat caatgaaatc caaaagaaaa
    24661 tttttccaat aagtgggaag aagttcccgg cagttacagt gatgacccca aatccaacgt
    24721 aaatgataaa ccagcttgga taaattgctt caattgaaag atcagctttg ataacatggt
    24781 gataattgaa ataaaagaca aggatcactt ggaagataac agcgcacagc caaatgaatt
    24841 tgacaattgg tgcaatactt tcatatgaaa caaaataagt acaaataacc atgagtgaca
    24901 tcgtaaaagt tggtgaaacg gaggcgatga ttggatcatg caacgtttgt ttcgtgtgtt
    24961 cgaataagat aaacaattta acaaaaatga atatcataag aatcatagag ataccgccag
    25021 ttagattgcc taaagcaaca tggtgatagt ctttaaacag atttccaagg gaagctaacc
    25081 caaggatcaa cccgcagatc gggatcggaa taatt
//
LOCUS       DAJSNV010000032        24607 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSNV010000032 DAJSNV010000000
VERSION     DAJSNV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.033"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(177..1025)
                     /locus_tag="ON516_001268"
     CDS             complement(177..1025)
                     /locus_tag="ON516_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3344761.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1249..3189)
                     /gene="thrS"
                     /locus_tag="ON516_001269"
     CDS             complement(1249..3189)
                     /gene="thrS"
                     /locus_tag="ON516_001269"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3344762.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3526..3705)
                     /locus_tag="ON516_001270"
     CDS             complement(3526..3705)
                     /locus_tag="ON516_001270"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3344763.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3752..5575)
                     /gene="cadA"
                     /locus_tag="ON516_001271"
     CDS             complement(3752..5575)
                     /gene="cadA"
                     /locus_tag="ON516_001271"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3344764.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5853..8132
                     /locus_tag="ON516_001272"
     CDS             5853..8132
                     /locus_tag="ON516_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3344765.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8225..8872)
                     /locus_tag="ON516_001273"
     CDS             complement(8225..8872)
                     /locus_tag="ON516_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344766.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9051..9770)
                     /locus_tag="ON516_001274"
     CDS             complement(9051..9770)
                     /locus_tag="ON516_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3344767.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9901..11052)
                     /locus_tag="ON516_001275"
     CDS             complement(9901..11052)
                     /locus_tag="ON516_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3344768.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11269..12009)
                     /locus_tag="ON516_001276"
     CDS             complement(11269..12009)
                     /locus_tag="ON516_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3344769.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12013..12336)
                     /gene="rsfS"
                     /locus_tag="ON516_001277"
     CDS             complement(12013..12336)
                     /gene="rsfS"
                     /locus_tag="ON516_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3344770.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12393..12986)
                     /gene="yqeK"
                     /locus_tag="ON516_001278"
     CDS             complement(12393..12986)
                     /gene="yqeK"
                     /locus_tag="ON516_001278"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3344771.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12970..13620)
                     /locus_tag="ON516_001279"
     CDS             complement(12970..13620)
                     /locus_tag="ON516_001279"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3344772.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13630..13941)
                     /gene="yhbY"
                     /locus_tag="ON516_001280"
     CDS             complement(13630..13941)
                     /gene="yhbY"
                     /locus_tag="ON516_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3344773.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13957..15066)
                     /gene="yqeH"
                     /locus_tag="ON516_001281"
     CDS             complement(13957..15066)
                     /gene="yqeH"
                     /locus_tag="ON516_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3344774.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15063..15590)
                     /locus_tag="ON516_001282"
     CDS             complement(15063..15590)
                     /locus_tag="ON516_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3344775.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15770..16555)
                     /locus_tag="ON516_001283"
     CDS             complement(15770..16555)
                     /locus_tag="ON516_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3344776.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16548..17417)
                     /locus_tag="ON516_001284"
     CDS             complement(16548..17417)
                     /locus_tag="ON516_001284"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3344777.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17418..18797)
                     /locus_tag="ON516_001285"
     CDS             complement(17418..18797)
                     /locus_tag="ON516_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3344778.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18805..19224)
                     /gene="fabZ"
                     /locus_tag="ON516_001286"
     CDS             complement(18805..19224)
                     /gene="fabZ"
                     /locus_tag="ON516_001286"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3344779.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19249..19725)
                     /gene="accB"
                     /locus_tag="ON516_001287"
     CDS             complement(19249..19725)
                     /gene="accB"
                     /locus_tag="ON516_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3344780.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19728..20963)
                     /gene="fabF"
                     /locus_tag="ON516_001288"
     CDS             complement(19728..20963)
                     /gene="fabF"
                     /locus_tag="ON516_001288"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3344781.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20982..21719)
                     /gene="fabG"
                     /locus_tag="ON516_001289"
     CDS             complement(20982..21719)
                     /gene="fabG"
                     /locus_tag="ON516_001289"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3344782.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21731..22648)
                     /gene="fabD"
                     /locus_tag="ON516_001290"
     CDS             complement(21731..22648)
                     /gene="fabD"
                     /locus_tag="ON516_001290"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3344783.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22755..22979)
                     /locus_tag="ON516_001291"
     CDS             complement(22755..22979)
                     /locus_tag="ON516_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3344784.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23032..23994)
                     /locus_tag="ON516_001292"
     CDS             complement(23032..23994)
                     /locus_tag="ON516_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3344785.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24004..24444)
                     /locus_tag="ON516_001293"
     CDS             complement(24004..24444)
                     /locus_tag="ON516_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3344786.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6748 a         5458 c         4269 g         8132 t
ORIGIN      
        1 ttttgtttca cagcaatcac ttaaagttac atatccaact atttttttca ttaaaaaata
       61 aacttatttt tgaaaattat gttttcttaa gtcgtgaaac actttgatgg atcatttatt
      121 tgtggaaaac tacgttttgt tattctaaaa aacttatata aaaaatagta aggtttttat
      181 ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc
      241 ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa
      301 agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt
      361 acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata
      421 ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa
      481 acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata
      541 tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact
      601 actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct
      661 acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt
      721 gcattgttac caagtttgtt tttgagagag taaaaggact catttgcttc tatatcacta
      781 aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga
      841 ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct
      901 tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg
      961 gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg actaattacg
     1021 ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt
     1081 attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat
     1141 cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat
     1201 tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct
     1261 gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc
     1321 tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc
     1381 aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt
     1441 ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc
     1501 ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc
     1561 caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat
     1621 tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc
     1681 aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt
     1741 ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc
     1801 ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc
     1861 ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg
     1921 atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa
     1981 ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc
     2041 attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg
     2101 atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt
     2161 tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc
     2221 tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt
     2281 gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc
     2341 tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa
     2401 tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt
     2461 acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt
     2521 gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca
     2581 ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg
     2641 tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc
     2701 tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc
     2761 ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc
     2821 tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta
     2881 gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg
     2941 catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg
     3001 gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag
     3061 ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc
     3121 aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat
     3181 ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa
     3241 acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac
     3301 ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc
     3361 aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct
     3421 tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt
     3481 ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc
     3541 cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt
     3601 tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg
     3661 ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct
     3721 ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat
     3781 caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca
     3841 cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca
     3901 tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat
     3961 ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat
     4021 ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag
     4081 cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt
     4141 tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat
     4201 ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca
     4261 tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa
     4321 tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg
     4381 tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat
     4441 gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat
     4501 acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct
     4561 gttctgcact agctgcatac agaagtagct cttctttttt tattccattg actggctgaa
     4621 tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt
     4681 ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg
     4741 aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg
     4801 ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac
     4861 cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc
     4921 gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg
     4981 cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa
     5041 tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga
     5101 ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta
     5161 gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga
     5221 atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt
     5281 tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag
     5341 cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc
     5401 catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc
     5461 ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta
     5521 ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt
     5581 tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat
     5641 cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta
     5701 tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta
     5761 ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat
     5821 tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc
     5881 acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac
     5941 aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag
     6001 ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat
     6061 tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca
     6121 tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat
     6181 tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc
     6241 gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg
     6301 aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac
     6361 gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat
     6421 tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa
     6481 atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg
     6541 gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc
     6601 tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga
     6661 ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg
     6721 tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc
     6781 taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg
     6841 atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg
     6901 gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat
     6961 tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc
     7021 tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag
     7081 aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta
     7141 aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga
     7201 attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt
     7261 ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc
     7321 gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga
     7381 tgcgccgaac atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa
     7441 tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt
     7501 tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat
     7561 tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct
     7621 ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa
     7681 caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat
     7741 tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag
     7801 caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt
     7861 tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt
     7921 tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag
     7981 atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg
     8041 cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg
     8101 atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt
     8161 tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg
     8221 tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa
     8281 taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac
     8341 ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa
     8401 ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg
     8461 ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga
     8521 taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca
     8581 tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa
     8641 tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac
     8701 ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg
     8761 cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc
     8821 ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc
     8881 ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct catcatacca
     8941 caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga
     9001 ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct
     9061 aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt
     9121 ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg
     9181 atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg
     9241 aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca
     9301 tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca
     9361 aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca
     9421 tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta
     9481 tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt
     9541 tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta
     9601 taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca
     9661 cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta
     9721 gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt
     9781 cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat
     9841 cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat
     9901 ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata
     9961 gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga
    10021 ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag
    10081 tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa
    10141 gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga
    10201 cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc
    10261 ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg
    10321 caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt
    10381 ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt
    10441 tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg
    10501 gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc
    10561 caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac
    10621 ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc
    10681 tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc
    10741 ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc
    10801 tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc
    10861 cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc
    10921 tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg
    10981 tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc
    11041 gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta
    11101 aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat
    11161 acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt
    11221 tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta
    11281 tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa
    11341 tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata
    11401 ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc
    11461 aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca
    11521 caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac
    11581 ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac
    11641 ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa
    11701 acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa
    11761 ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga
    11821 aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt
    11881 accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc
    11941 taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc
    12001 ataagccata tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag
    12061 tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc
    12121 aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt
    12181 gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt
    12241 tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat
    12301 aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact
    12361 atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat
    12421 gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt
    12481 tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt
    12541 accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg
    12601 agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg
    12661 gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta
    12721 ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca
    12781 tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa
    12841 gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag
    12901 cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta
    12961 tattcattac tatagtccat attcatacaa cccttcattt tgaatataat caattacttt
    13021 atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga
    13081 actgatgtcg atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta
    13141 tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca
    13201 ttttggcaaa tattctacca tatccccacc gataataaag taataatctg tatccggatt
    13261 gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc
    13321 aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg
    13381 atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa
    13441 aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag
    13501 gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg
    13561 aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat
    13621 ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga
    13681 aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt
    13741 ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt
    13801 gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc
    13861 accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt
    13921 ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa
    13981 tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca
    14041 gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag
    14101 ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt
    14161 ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac
    14221 ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa
    14281 gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc
    14341 aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc
    14401 cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt
    14461 tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg
    14521 attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc
    14581 gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt
    14641 taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt
    14701 taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac
    14761 tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat
    14821 atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa
    14881 gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg
    14941 ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc
    15001 taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc
    15061 tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata
    15121 attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt
    15181 tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga
    15241 tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc
    15301 cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct
    15361 cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc
    15421 atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac
    15481 gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta
    15541 atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct
    15601 catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat
    15661 cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa
    15721 tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc
    15781 ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca
    15841 atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc
    15901 cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag
    15961 taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg
    16021 caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg
    16081 ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg
    16141 gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct
    16201 cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat
    16261 tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag
    16321 aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg
    16381 ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata
    16441 aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg
    16501 tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct
    16561 atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg
    16621 cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct
    16681 tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag
    16741 ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg
    16801 gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca
    16861 gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca
    16921 cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg
    16981 gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact
    17041 ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt
    17101 cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct
    17161 ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga
    17221 cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg
    17281 atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga
    17341 acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt
    17401 ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa
    17461 aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc
    17521 aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct
    17581 cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc
    17641 attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt
    17701 cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg
    17761 aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct
    17821 tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg
    17881 attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg
    17941 aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg
    18001 actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc
    18061 actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc
    18121 tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga
    18181 gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct
    18241 tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct
    18301 ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt
    18361 tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt
    18421 gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt
    18481 ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca
    18541 gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt
    18601 acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca
    18661 tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg
    18721 atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc
    18781 aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac
    18841 ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa
    18901 tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt
    18961 gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac
    19021 tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg
    19081 aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc
    19141 tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat
    19201 ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg
    19261 aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt
    19321 gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca
    19381 gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc
    19441 tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc
    19501 cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac
    19561 tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt
    19621 atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga
    19681 ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag
    19741 cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa
    19801 gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt
    19861 ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag
    19921 gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg
    19981 taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca
    20041 ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca
    20101 gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct
    20161 gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca
    20221 ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc
    20281 acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct
    20341 gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt
    20401 cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt
    20461 gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca
    20521 cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa
    20581 actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca
    20641 ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa
    20701 ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt
    20761 cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct
    20821 aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc
    20881 tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag
    20941 ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat
    21001 ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct
    21061 gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt
    21121 tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg
    21181 gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact
    21241 acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca
    21301 ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc
    21361 gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc
    21421 attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat
    21481 ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca
    21541 ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga
    21601 atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc
    21661 gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata
    21721 acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca
    21781 gaactggtat tgttttatcg atttttttca taaatccgct taatactttt ccggggccga
    21841 cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa
    21901 caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg
    21961 ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct
    22021 tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta
    22081 gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt
    22141 tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc
    22201 cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg
    22261 caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta
    22321 ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc
    22381 ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat
    22441 acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat
    22501 tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg
    22561 tctgctttac aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt
    22621 ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt
    22681 gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa
    22741 cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt
    22801 ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt
    22861 tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt
    22921 tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata
    22981 ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc
    23041 catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg
    23101 ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc
    23161 agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac
    23221 ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac
    23281 tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa
    23341 aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg
    23401 atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa
    23461 tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc
    23521 gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc
    23581 accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc
    23641 ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa
    23701 tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac
    23761 taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac
    23821 taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt
    23881 ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat
    23941 aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata
    24001 tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg
    24061 ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg
    24121 atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc
    24181 atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt
    24241 caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta
    24301 catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt
    24361 tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt
    24421 tactgttttt aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc
    24481 ttttgatgtt caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt
    24541 ttactatatg agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt
    24601 ataattt
//
LOCUS       DAJSNV010000033        24575 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSNV010000033 DAJSNV010000000
VERSION     DAJSNV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.034"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(154..552)
                     /locus_tag="ON516_001294"
     CDS             complement(154..552)
                     /locus_tag="ON516_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3344787.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="ON516_001295"
     CDS             complement(549..1322)
                     /locus_tag="ON516_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3344788.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="ON516_001296"
     CDS             complement(1319..2002)
                     /locus_tag="ON516_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3344789.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="ON516_001297"
     CDS             complement(2169..3056)
                     /locus_tag="ON516_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3344790.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="ON516_001298"
     CDS             complement(3053..3922)
                     /locus_tag="ON516_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344791.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="ON516_001299"
     CDS             complement(4059..5345)
                     /locus_tag="ON516_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3344792.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="ON516_001300"
     CDS             5618..6196
                     /locus_tag="ON516_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3344793.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="ON516_001301"
     CDS             6196..7125
                     /locus_tag="ON516_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3344794.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="ON516_001302"
     CDS             7122..8858
                     /locus_tag="ON516_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3344795.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ON516_001303"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ON516_001303"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3344796.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="ON516_001304"
     CDS             complement(9754..10263)
                     /locus_tag="ON516_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3344797.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ON516_001305"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ON516_001305"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3344798.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="ON516_001306"
     CDS             complement(11564..12655)
                     /locus_tag="ON516_001306"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3344799.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ON516_001307"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ON516_001307"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3344800.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ON516_001308"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ON516_001308"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3344801.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ON516_001309"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ON516_001309"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3344802.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="ON516_001310"
     CDS             complement(16618..17592)
                     /locus_tag="ON516_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3344803.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="ON516_001311"
     CDS             complement(17573..19456)
                     /locus_tag="ON516_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3344804.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="ON516_001312"
     CDS             complement(19474..21699)
                     /locus_tag="ON516_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3344805.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="ON516_001313"
     CDS             21814..22848
                     /locus_tag="ON516_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3344806.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="ON516_001314"
     CDS             complement(22915..23928)
                     /locus_tag="ON516_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3344807.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="ON516_001315"
     CDS             complement(24006..24194)
                     /locus_tag="ON516_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3344808.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaac
//
LOCUS       DAJSNV010000034        24541 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSNV010000034 DAJSNV010000000
VERSION     DAJSNV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.035"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            67..444
                     /locus_tag="ON516_001316"
     CDS             67..444
                     /locus_tag="ON516_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344809.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(624..1919)
                     /gene="purB"
                     /locus_tag="ON516_001317"
     CDS             complement(624..1919)
                     /gene="purB"
                     /locus_tag="ON516_001317"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3344810.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1932..3056)
                     /gene="purK"
                     /locus_tag="ON516_001318"
     CDS             complement(1932..3056)
                     /gene="purK"
                     /locus_tag="ON516_001318"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3344811.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3049..3537)
                     /gene="purE"
                     /locus_tag="ON516_001319"
     CDS             complement(3049..3537)
                     /gene="purE"
                     /locus_tag="ON516_001319"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3344812.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3830..5137)
                     /locus_tag="ON516_001320"
     CDS             complement(3830..5137)
                     /locus_tag="ON516_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3344813.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5140..5721)
                     /locus_tag="ON516_001321"
     CDS             complement(5140..5721)
                     /locus_tag="ON516_001321"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3344814.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5837..5934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6293..7585)
                     /locus_tag="ON516_001322"
     CDS             complement(6293..7585)
                     /locus_tag="ON516_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3344815.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7582..8205)
                     /locus_tag="ON516_001323"
     CDS             complement(7582..8205)
                     /locus_tag="ON516_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344816.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8208..9044)
                     /locus_tag="ON516_001324"
     CDS             complement(8208..9044)
                     /locus_tag="ON516_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3344817.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9061..9948)
                     /locus_tag="ON516_001325"
     CDS             complement(9061..9948)
                     /locus_tag="ON516_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3344818.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9956..11191)
                     /locus_tag="ON516_001326"
                     /pseudo
     CDS             complement(9956..11191)
                     /locus_tag="ON516_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11874..12557)
                     /locus_tag="ON516_001327"
     CDS             complement(11874..12557)
                     /locus_tag="ON516_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3344819.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12575..13192)
                     /locus_tag="ON516_001328"
     CDS             complement(12575..13192)
                     /locus_tag="ON516_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3344820.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13202..14026)
                     /locus_tag="ON516_001329"
     CDS             complement(13202..14026)
                     /locus_tag="ON516_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3344821.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14019..14579)
                     /locus_tag="ON516_001330"
     CDS             complement(14019..14579)
                     /locus_tag="ON516_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344822.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14753..15709
                     /locus_tag="ON516_001331"
     CDS             14753..15709
                     /locus_tag="ON516_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3344823.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16014..16970)
                     /locus_tag="ON516_001332"
     CDS             complement(16014..16970)
                     /locus_tag="ON516_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3344824.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17074..17676
                     /locus_tag="ON516_001333"
     CDS             17074..17676
                     /locus_tag="ON516_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3344825.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17764..19263)
                     /locus_tag="ON516_001334"
     CDS             complement(17764..19263)
                     /locus_tag="ON516_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3344826.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19279..19803)
                     /locus_tag="ON516_001335"
     CDS             complement(19279..19803)
                     /locus_tag="ON516_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3344827.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20101..20850
                     /locus_tag="ON516_001336"
     CDS             20101..20850
                     /locus_tag="ON516_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3344828.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20841..21626
                     /locus_tag="ON516_001337"
     CDS             20841..21626
                     /locus_tag="ON516_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3344829.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21595..22311
                     /locus_tag="ON516_001338"
     CDS             21595..22311
                     /locus_tag="ON516_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3344830.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22413..23447)
                     /locus_tag="ON516_001339"
     CDS             complement(22413..23447)
                     /locus_tag="ON516_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3344831.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23451..24509)
                     /locus_tag="ON516_001340"
     CDS             complement(23451..24509)
                     /locus_tag="ON516_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3344832.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7293 a         5266 c         4287 g         7695 t
ORIGIN      
        1 tacttaatga tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac
       61 ttgccgatgg aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa
      121 gaacgttcct gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa
      181 gatactagtt tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc
      241 cgtaaaagga cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt
      301 catgaacaga agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag
      361 atggaatacg cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat
      421 aatagccaag ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa
      481 gaggtagccg cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa
      541 gaagcagaac cgctaacgga tctgcttctt ttttggttat ttttgctatt ctattttaat
      601 tgaattttgc aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg
      661 tttttcaaat gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat
      721 gtgatggttg gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac
      781 gcagtttttg gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat
      841 ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg
      901 ttttcaggaa agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc
      961 aataaaatcg tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt
     1021 tcatgccata atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct
     1081 agaccagcca tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct
     1141 ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt
     1201 atctctgttg caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca
     1261 gcatgtagat cccgagggag aacttgagta gagatttctt gtggacggat ccccaattgt
     1321 ttacatacat actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag
     1381 atttttcctg cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt
     1441 tctgaatacc atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt
     1501 gtccgaccca tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta
     1561 aaacgtttca agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca
     1621 gtatctacga catccgtact tgttagacca taatggaccc attttctttc ttctcccaat
     1681 gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc
     1741 aagatccgat cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg
     1801 atttcaccaa gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg
     1861 tatttgttct cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg
     1921 ttttttctcc tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg
     1981 tcttctgtca agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca
     2041 tagaaatgga attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt
     2101 tctcccaaaa cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc
     2161 caaccacaaa tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa
     2221 taatgtccag aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt
     2281 gttaaaaaca tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg
     2341 gcaatacgct cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca
     2401 tgaagaatat tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga
     2461 ccatttcctg ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca
     2521 caggttcctt ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca
     2581 tactgcccct tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc
     2641 ccgtcaatcg catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg
     2701 atgttattcg tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag
     2761 tctgtccctt gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg
     2821 ttttcgaatt cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg
     2881 tcatcataga cactttcaat gtgccaatct gcaacttgtg cggctggaca atcgctcaca
     2941 ggatccagca cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct
     3001 aattgaccgc caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg
     3061 atcactactt tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc
     3121 caattctaaa tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc
     3181 cgcttttccg attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag
     3241 aagcgaatcc aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt
     3301 tgtttttgat gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat
     3361 tttgattcct ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg
     3421 agcagaaacg accttttttt cataagacac accgaattca tccaatattt gacatgcttc
     3481 tttcattgtt tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt
     3541 ttttcctccc agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa
     3601 atttcgaatg ttaacaaaga aaaacatttt attgttcgtg tttattaaag atgaaaaaaa
     3661 ggaatgtgac aaaagttagg tcacattcct ttttatggat aaacggtgtt caatcagctg
     3721 accctcggta ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc
     3781 tctaacaatc aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga
     3841 aggcttcttc caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga
     3901 tagccgtaat gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca
     3961 tttgactgaa taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa
     4021 caatcaatag atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga
     4081 ctgccaccat cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt
     4141 gtgctaacgc accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat
     4201 agatgggttt tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc
     4261 cagtataagg gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat
     4321 atcccttttt caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat
     4381 atacacctgt tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc
     4441 atacgtcgaa agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat
     4501 ctccaatcgc gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta
     4561 aaccaatcaa taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa
     4621 ttaggagaat cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg
     4681 ttgcattacc tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg
     4741 tgataaccgt tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag
     4801 aaactaacac aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt
     4861 cattggttcc gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag
     4921 gcaagccaat tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca
     4981 taaagatatc aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac
     5041 cgatcaaaag aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta
     5101 aaaccgcagc ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa
     5161 ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat
     5221 ttcttcaagt aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc
     5281 tacttgcgct ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc
     5341 caagaaatcg tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga
     5401 aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc
     5461 catcgtcaaa ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc
     5521 aaataaagct ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc
     5581 tttaaatact tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg
     5641 agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat
     5701 acgttcaact aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac
     5761 cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat
     5821 aagaaaagaa gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc
     5881 tagaaactgt catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg
     5941 taaattcatt ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa
     6001 aatgttgttt caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt
     6061 tacttatttt tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt
     6121 aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga
     6181 caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg
     6241 caataaggca agattttata aactacggga agtaatttcc gtaaatctat tcttattctt
     6301 ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg
     6361 cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat
     6421 cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc
     6481 cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga
     6541 tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact
     6601 tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt
     6661 cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg
     6721 ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat
     6781 atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt
     6841 ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt
     6901 ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata
     6961 ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat
     7021 gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc
     7081 cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac
     7141 tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc
     7201 gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga
     7261 tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat
     7321 catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta
     7381 gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa
     7441 gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac
     7501 aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc
     7561 cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac
     7621 tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca
     7681 ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa
     7741 cagccagata ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat
     7801 atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg
     7861 aatcagatac caagctccaa tctttcccac taataaatac caatcaactg caaaaataat
     7921 gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca
     7981 acataccttt ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa
     8041 aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc
     8101 tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata
     8161 gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga
     8221 ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc
     8281 ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg
     8341 tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc
     8401 aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc
     8461 gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca
     8521 ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa
     8581 atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa
     8641 ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc
     8701 gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg
     8761 taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac
     8821 gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa
     8881 ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc
     8941 gttttaaaag aacctgcgat catccataag accggataaa tcatgactag acctaatagc
     9001 aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat
     9061 tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat
     9121 caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg
     9181 gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat
     9241 aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat
     9301 gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc
     9361 gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat
     9421 cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg
     9481 atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc
     9541 gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact
     9601 aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata
     9661 agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt
     9721 gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc
     9781 gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat
     9841 catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc
     9901 gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg
     9961 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
    10021 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
    10081 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
    10141 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
    10201 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
    10261 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
    10321 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
    10381 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
    10441 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
    10501 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
    10561 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
    10621 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
    10681 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
    10741 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
    10801 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
    10861 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
    10921 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
    10981 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
    11041 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
    11101 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
    11161 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
    11221 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
    11281 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
    11341 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
    11401 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
    11461 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
    11521 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
    11581 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
    11641 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
    11701 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
    11761 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
    11821 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
    11881 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
    11941 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
    12001 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
    12061 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
    12121 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
    12181 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
    12241 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
    12301 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
    12361 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
    12421 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
    12481 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
    12541 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
    12601 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
    12661 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
    12721 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
    12781 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
    12841 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
    12901 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
    12961 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
    13021 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
    13081 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
    13141 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
    13201 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
    13261 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
    13321 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
    13381 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
    13441 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
    13501 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
    13561 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
    13621 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
    13681 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
    13741 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
    13801 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
    13861 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
    13921 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
    13981 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
    14041 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
    14101 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
    14161 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
    14221 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
    14281 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
    14341 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
    14401 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
    14461 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
    14521 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
    14581 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
    14641 ctgctttcat tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt
    14701 ataataatag acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa
    14761 atatcaatgg ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa
    14821 tcaagagacg agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt
    14881 cgctcaccgt tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc
    14941 tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc
    15001 agcattggaa gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga
    15061 actggctgac gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac
    15121 aatcttcaac atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg
    15181 ggctttaaaa atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg
    15241 gttcttcaat ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc
    15301 tttcgctcgt ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt
    15361 tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc
    15421 tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa
    15481 cggttatgtg acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga
    15541 tggtacaaag gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa
    15601 gaatatggta cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga
    15661 tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt
    15721 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta
    15781 tttttcgttt ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag
    15841 aggttgtgag cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct
    15901 catgtttgtt gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag
    15961 aagaggttgt gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt
    16021 tggatttttt gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa
    16081 aactggaaca attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc
    16141 cgatacaata cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct
    16201 gaaaatgttt ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt
    16261 tgctggattc tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc
    16321 atagagaagt aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct
    16381 ctggcatctt cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca
    16441 tcttctttat tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct
    16501 ttttcgatct gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt
    16561 gcaagcaaag caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc
    16621 caagacatgt tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct
    16681 tcggttaatt tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca
    16741 aaaatagtat acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag
    16801 gctgcgattc gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat
    16861 aatgcaatga tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc
    16921 gaaaagaaga cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc
    16981 cttttttatt ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt
    17041 atgatagtaa aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt
    17101 atgcgtacga tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact
    17161 caacgaggaa tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg
    17221 actacaaccg tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta
    17281 gctatcattt atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc
    17341 ttgcttccta ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc
    17401 ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa
    17461 tccgctttgt atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc
    17521 ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat
    17581 ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct
    17641 aagatccaaa aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa
    17701 aatggagaga agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact
    17761 actttattct tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac
    17821 ttggctagta gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt
    17881 tgtcgctttt agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg
    17941 gttcgctcca ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat
    18001 atttcctagc ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact
    18061 caagacagct gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc
    18121 ttctgctttt ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt
    18181 attggcttcc gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac
    18241 ttctttgact ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt
    18301 tttcttcact tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc
    18361 gatttgtttt tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg
    18421 tgctcgggca ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc
    18481 ttgtttgtag gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg
    18541 tcgctgtaat tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc
    18601 ttttagagct tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt
    18661 ccctaaagag tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat
    18721 gatcaacccc atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt
    18781 atcgcgattt tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc
    18841 ttctaatact tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc
    18901 tgctgctgta gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca
    18961 catcaccggt acgccttgtt ccgtatacac ttcaggggtg gagacgtcca atttactcga
    19021 taatagactg atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg
    19081 cacaattttg attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact
    19141 tccactgatg atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat
    19201 cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc
    19261 catttaacac acgctccttt aagcttttat catgctttca taaggcatga cataacagat
    19321 tctttccttg atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg
    19381 gctgtaaaaa caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc
    19441 catacctcgt acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc
    19501 agaagacgac atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa
    19561 aaataccaac actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc
    19621 tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag
    19681 catcggatca agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc
    19741 caatcctgca caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat
    19801 catatacacg cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata
    19861 gcttctttta ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt
    19921 tgttaaattt ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg
    19981 tttccttttt ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt
    20041 ttctccacat tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg
    20101 atgaaccgat ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat
    20161 aaactcatcc aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca
    20221 aatgaaatca tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt
    20281 cgatattctc acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg
    20341 aaaaaagtga ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc
    20401 ttccgctatt tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc
    20461 ttaacaaaca acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca
    20521 ttgattattg gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa
    20581 aaagtactgc ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt
    20641 atcgaaaatg aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg
    20701 atcaatacac ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta
    20761 ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa
    20821 cgaccgttcg gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac
    20881 ctctttttag caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag
    20941 taatgaaatt aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc
    21001 aaacccatga attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt
    21061 atgaagaagt cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc
    21121 aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg
    21181 aagccaaaga acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg
    21241 agatggatga ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa
    21301 aagaagccgt gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg
    21361 acacagaagc tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag
    21421 aaatctcaga aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg
    21481 gattgaatct catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac
    21541 aaacaaatgg cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg
    21601 acaaatgtcc tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg
    21661 gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa
    21721 tatttcagac aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt
    21781 ggtatccatt ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc
    21841 actgctgcag atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat
    21901 ttgatcaaac cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc
    21961 tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac
    22021 cacaccactc ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa
    22081 ctgactttaa aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa
    22141 gaactggctc aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta
    22201 gtagaacaag atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc
    22261 tgttatgaat tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa
    22321 ttatcccata caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt
    22381 taaacatttt attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga
    22441 gatacctcat ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc
    22501 gaaatcatat ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact
    22561 ctagggtaca tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg
    22621 aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa
    22681 tgattgcttt tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta
    22741 ttaatcaaga tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg
    22801 ttttcacctg ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct
    22861 tcaagcgagt ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg
    22921 atcactttgg ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt
    22981 cctatgatcc ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga
    23041 aaatctttga aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca
    23101 aaagtcaaag ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc
    23161 ataccaaaat cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa
    23221 acataacgaa tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt
    23281 cccctcaaca atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt
    23341 tcttcataaa gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg
    23401 ttcggacgaa caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat
    23461 aaacctatgc catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca
    23521 aaaatagttg acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata
    23581 gcataggctg ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt
    23641 gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc
    23701 aatgcaggta caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca
    23761 gcttttttga agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa
    23821 cttaaaccag ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc
    23881 aaaaacgcta cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac
    23941 ccttggaaca gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac
    24001 atcgctttga tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa
    24061 tacaaaaaga ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca
    24121 taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca
    24181 cgcaacgttg tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata
    24241 agaataatat aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct
    24301 gtattgatat cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg
    24361 actttcgata gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac
    24421 cctaataata taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa
    24481 gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca
    24541 c
//
LOCUS       DAJSNV010000035        24436 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSNV010000035 DAJSNV010000000
VERSION     DAJSNV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.036"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(55..789)
                     /locus_tag="ON516_001341"
     CDS             complement(55..789)
                     /locus_tag="ON516_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3344833.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ON516_001342"
     CDS             1116..1526
                     /locus_tag="ON516_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3344834.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ON516_001343"
     CDS             1519..2220
                     /locus_tag="ON516_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3344835.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ON516_001344"
     CDS             2220..3806
                     /locus_tag="ON516_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344836.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ON516_001345"
     CDS             3825..4046
                     /locus_tag="ON516_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3344837.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ON516_001346"
     CDS             4043..4804
                     /locus_tag="ON516_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3344838.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ON516_001347"
     CDS             5184..5693
                     /locus_tag="ON516_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3344839.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ON516_001348"
     CDS             complement(5768..6307)
                     /locus_tag="ON516_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3344840.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ON516_001349"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ON516_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3344841.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ON516_001350"
     CDS             7320..8171
                     /locus_tag="ON516_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3344842.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ON516_001351"
     CDS             8199..8834
                     /locus_tag="ON516_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3344843.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ON516_001352"
     CDS             8854..9627
                     /locus_tag="ON516_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3344844.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ON516_001353"
     CDS             10035..10832
                     /locus_tag="ON516_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3344845.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ON516_001354"
     CDS             10810..11223
                     /locus_tag="ON516_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3344846.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ON516_001355"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ON516_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3344847.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ON516_001356"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ON516_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3344848.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="ON516_001357"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="ON516_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3344849.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ON516_001358"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ON516_001358"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3344850.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ON516_001359"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ON516_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3344851.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ON516_001360"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ON516_001360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3344852.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ON516_001361"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ON516_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3344853.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ON516_001362"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ON516_001362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3344854.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ON516_001363"
     CDS             21907..22485
                     /locus_tag="ON516_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3344855.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ON516_001364"
     CDS             22537..23019
                     /locus_tag="ON516_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3344856.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ON516_001365"
     CDS             23136..23510
                     /locus_tag="ON516_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344857.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24436
                     /locus_tag="ON516_001366"
     CDS             23940..>24436
                     /locus_tag="ON516_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3344858.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         8010 a         4391 c         5216 g         6819 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataagag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga
    24301 tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc
    24361 tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
    24421 ttatggacgc tactta
//
LOCUS       DAJSNV010000036        24412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSNV010000036 DAJSNV010000000
VERSION     DAJSNV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.037"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(66..1328)
                     /locus_tag="ON516_001367"
     CDS             complement(66..1328)
                     /locus_tag="ON516_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344859.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1321..2220)
                     /locus_tag="ON516_001368"
     CDS             complement(1321..2220)
                     /locus_tag="ON516_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344860.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ON516_001369"
     CDS             complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ON516_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3344861.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ON516_001370"
     CDS             complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ON516_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3344862.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4726..7086)
                     /locus_tag="ON516_001371"
     CDS             complement(4726..7086)
                     /locus_tag="ON516_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3344863.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7201..7749)
                     /locus_tag="ON516_001372"
     CDS             complement(7201..7749)
                     /locus_tag="ON516_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3344864.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="ON516_001373"
     CDS             complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="ON516_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3344865.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8446..9363
                     /locus_tag="ON516_001374"
     CDS             8446..9363
                     /locus_tag="ON516_001374"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3344866.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9741..10652)
                     /locus_tag="ON516_001375"
     CDS             complement(9741..10652)
                     /locus_tag="ON516_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3344867.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11053..11631)
                     /locus_tag="ON516_001376"
     CDS             complement(11053..11631)
                     /locus_tag="ON516_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3344868.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11651..12319)
                     /locus_tag="ON516_001377"
     CDS             complement(11651..12319)
                     /locus_tag="ON516_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344869.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12334..13404)
                     /locus_tag="ON516_001378"
     CDS             complement(12334..13404)
                     /locus_tag="ON516_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344870.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13575..14939)
                     /locus_tag="ON516_001379"
     CDS             complement(13575..14939)
                     /locus_tag="ON516_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3344871.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14936..16495)
                     /locus_tag="ON516_001380"
     CDS             complement(14936..16495)
                     /locus_tag="ON516_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344872.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16551..19031)
                     /locus_tag="ON516_001381"
     CDS             complement(16551..19031)
                     /locus_tag="ON516_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344873.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(19015..19350)
                     /locus_tag="ON516_001382"
     CDS             complement(19015..19350)
                     /locus_tag="ON516_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344874.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19364..20398)
                     /locus_tag="ON516_001383"
     CDS             complement(19364..20398)
                     /locus_tag="ON516_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3344875.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20488..21168)
                     /locus_tag="ON516_001384"
     CDS             complement(20488..21168)
                     /locus_tag="ON516_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3344876.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21338..22297)
                     /locus_tag="ON516_001385"
     CDS             complement(21338..22297)
                     /locus_tag="ON516_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3344877.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(22518..23621)
                     /locus_tag="ON516_001386"
     CDS             complement(22518..23621)
                     /locus_tag="ON516_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3344878.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23843..24313)
                     /locus_tag="ON516_001387"
     CDS             complement(23843..24313)
                     /locus_tag="ON516_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3344879.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT         6916 a         5117 c         4168 g         8211 t
ORIGIN      
        1 aggtctatac aaaaagaagc tgggaacaat agatgtccag cttccttctg tattttagga
       61 ttgttttatg ctttttttcg ttcattcctc ataattgaaa tcgattgttt caatgaactc
      121 cataatccgc cttcgctata aacgagtaca ttcgatttgt acatgtttgc agacaagatc
      181 atcaataaga atgttacggc tacaagtacc aacaaggaaa tcaatgctcc acccataccg
      241 actgtctcat ttgctaagcg aatcggcata atatatgaag ataagaatgg aatatacgaa
      301 gtgatccgaa taacgatatt gtttggatct gaagctccta gtatcaatcc aagcatgtat
      361 ccgcctagag atagataagt cactggcaaa attgcttttg ctgtatcttc tgctttgttg
      421 accaaagagc cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa gatcatgaat
      481 agaagagaat accacaagaa tggtccaatc aagttatcca atgagatacc gtctaatata
      541 tttttgacca tttccatatc tttgaattga ctgtatccta taccaaaaat aataccgtat
      601 aatgccattt gcgttaatgc aacaagtaaa acacctgtta attttccgta aaaatgcttt
      661 tgtgcagtcg tactggacag gatcacttcc atgatccgcg tgcctttttc agaagcgatc
      721 tcttgtgcaa tgatctgcgc atacgtcaaa ataataatga acaagacaat cgttgctaca
      781 taacttacga cgtattggac tgtcgagtta tcttctccga ctgtcatttt tccttttgaa
      841 tcaaaattga ctttttgtcg agtcaaactt gcttgttggc tcaagctagc tacttcttct
      901 ggtgaaatgc ctaattgact cgctcgaagc atcgattgca agccagtcag ttgttgttgt
      961 atcagtagtt gtgtactttg tcctaatgaa ttttcgctat aaagcatccc atttatctta
     1021 tcattctcag ttgagactac taaatacgca tcgatttttt cttccgaaag ctctttttgt
     1081 gctttttctt cagaatcaac tgctttgaac tcaaaatcat ctgttttgac ttggctcatt
     1141 tgctgactga tttgctcatt atcagccaat acgccgattt tattgacatc actattttgt
     1201 tgagcaaagt ttcctgctac ataaatgata cccagcatga taaaagggac taatatcatg
     1261 atcaagaaag aaatcgactt aacatttttt aaatagacat ctttggtaat aatccaaaac
     1321 ttacgcatcg atttcacctg ctttcaattt gaaaatctct tccaaagttg gcggttgctg
     1381 gttaaacata ggaatgtagc cgaactgagt cgcacgagta aagatttctt tcccagcttc
     1441 aggatcttct aaggtaatct ctaacgtatt gtcttcatga aggatcactt ttttcacgcc
     1501 ttcgatttct tctacttcct gttggcttaa accagattcc aagaataact tcgtacggcc
     1561 aaatgattca cgaatctcat gtacttttcc attcaaaacc atttttccgt ttcgcaacat
     1621 gatcaaatga tcgcagattt tttctacatt atccatattg tggctagaaa aaatgacaca
     1681 tgaaccatgt tttttcaatt ccaatattcc atcttttagc aattcagcat ttaccggatc
     1741 taacccgctg aatggctcat ccaaaatgat caatttaggt tcatggatta acgtggcgat
     1801 cagctggact ttttgctgat ttcctttaga caaagatttt actttatccg tctttttccc
     1861 ttttacctga aatttttcca tccaaaagtc gatttttggt tcaatctctt ttcttgattt
     1921 ccctctaagt tctgcaaaat aaagcagttg gtcttgaatc gttactttcg gataaagtcc
     1981 ccgttcctcc ggaagataac cgatgacatc ataatctttc tccccaagat gatggccatt
     2041 ccataacact tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa aagtcgttgt
     2101 cttcccagcc ccgttttggc caattagccc catgattttt ccatcgggga tttggaaaga
     2161 gacatgatcg acagctgtgt agttcccaaa agttttaaca agatccttga cttctaacat
     2221 ttcttcactc cttcttcaag aaatcacata tatccttaga caccagacgt aagcgattta
     2281 ttatttacca acaaaacgta aacgctcttt ttgttgtaca taaaatgata ggacacttta
     2341 ctttctttgt aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc
     2401 gacatttttc ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt
     2461 caacgatttg aactctttaa gcagcatttt ttttcgtttt ggtcctaacc cttcaatttc
     2521 atctaatcgg gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa
     2581 tcgatggact tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag
     2641 cggtactaca gccaaatctg taccgaatag caactcattt gttttatgtt tatcgttttt
     2701 taccatgcca gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac
     2761 atctacttgt ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg
     2821 aatcactcgc gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc
     2881 gttgactgtt tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa
     2941 tacgaccatc gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat
     3001 acgtattggg gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg
     3061 ttcctgtttt ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttact
     3121 cgccaattca actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa
     3181 caaagcttct acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa
     3241 atgctcattt tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt
     3301 atagaaagga aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac
     3361 ttggacacac atccatcctt tatcaatcgc ataaccaaat acatctcgat ccagtaaatc
     3421 agtatttgtc attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta
     3481 ttctgcagct ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc
     3541 tgactcgatt ttttcgtgtc ctccgtttaa aaagtttttc acttcatcaa cgattttcgt
     3601 atagactttc ggatctacgt cgaaataata aggacacagg cattgtccca aatgatagta
     3661 aagacaaggc tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag
     3721 gatcttcttt gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt
     3781 gtcttttagt accttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag
     3841 gaaaggatag gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat
     3901 cagattgatc tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc
     3961 ttcgatttca ctaacaagcc gttccgtttt cgtattatgg ctaccagtaa aatatgagcg
     4021 gacacgattt tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt
     4081 catcaagtaa cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt
     4141 catctggtac tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca
     4201 atccagcctg acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac
     4261 cgcaaaaaaa ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa
     4321 gtttttataa atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag
     4381 ctttttctac tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga
     4441 attgttgagg agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt
     4501 catcatattc ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc
     4561 aagttgccca aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat
     4621 ctgtaattac ttgtgacata tcaaagacct ccatttttaa aatctatgac tagtatagca
     4681 tttctatact tggatagcga accatctgct tacctttagt aagctttact taaatttaac
     4741 aattgtcgca ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg
     4801 atggttttta agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac
     4861 aatagtcact tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact
     4921 tagcgcttct tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga
     4981 ttcagatcgt acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt
     5041 cagctcgtct tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc
     5101 gtttttacgt aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg
     5161 tttcaacgtt ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc
     5221 ttgatgtaga tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc
     5281 ttgcccaatc tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc
     5341 cgcctctgac acgacttcat ttgctttttt ctttgctttt tccatttctt tttcacgttc
     5401 ttcaaagaaa tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac
     5461 aaaatgacgc atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt
     5521 gatcatttcg tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac
     5581 ttctgtatca agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc
     5641 aatcagcaaa cgatacgttg gacttaatgt atccacatca aactccatgc tggcattaat
     5701 cgtatttgcc cgattatatc cataaacttt caattcagga taatgcgttg ttgccatcac
     5761 ataagcagac tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg
     5821 cggatctgtt cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga
     5881 taaaatatct acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga
     5941 ctgttcgtct cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc
     6001 tgcaggaatc ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt
     6061 gatcgttttc ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat
     6121 tgtgatgtca ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa
     6181 gacgacatga ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg
     6241 tgcttttgca ttcatcagat ccattttacc aatcacataa gtattgtgga tgatttcttg
     6301 acgatgggga acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc
     6361 agcgatttgg tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa
     6421 taaagtctga cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc
     6481 ttgtttgact gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata
     6541 ctttgcattt ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg
     6601 ccgaatgttc tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc
     6661 ttcatcgatt gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc
     6721 ccacatatac aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt
     6781 caattcgctt gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc
     6841 tgctccgatc tcaatccgtt tcatatgggg acggatattt tcaattttag gtatagggat
     6901 cccgcctcgc agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat
     6961 cgtttgtttc tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac
     7021 tacaaattgt ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt
     7081 attcatttta tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg
     7141 cagccttctt aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc
     7201 ttaattcaaa atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct
     7261 ctcaacgata aaacgagcga aactatgctc gccgaattga ttttggacca catctaatgg
     7321 aacaaaggca agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag
     7381 gatgcctcct gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac
     7441 aaaaaccccc acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt
     7501 tcctgcataa aaagcttcat ctaggtgaaa ggcaaatacc tgatcaaaaa atgctaactt
     7561 tgatgttggg gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt
     7621 gtaaaaatgc tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa
     7681 agcaaacccg cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa
     7741 actcagcatg ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa
     7801 aggatcttaa ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg
     7861 ttgtgcttcc acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc
     7921 ccgagcttcg ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt
     7981 gatcattttt ttctttaatt ctgctgtttc ttcttccagc tccaatattc tttcttgttt
     8041 cttcaattga tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat
     8101 ttgtggtgat aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc
     8161 catatgatgc ttggtttccc gtccgataat agtatatgtt tggttggcaa tcaccgcttt
     8221 gtagcgtgtt ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt
     8281 tagtgtacca tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac
     8341 tattatatga taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt
     8401 cgtgagttgc ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt
     8461 atcaaagcaa caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta
     8521 aataaacaga ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct
     8581 tatacatccg gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg
     8641 ggggaggcac aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat
     8701 atcagtcaat tatccgtttt aggtagtgat gaggtcggaa atggcagcta tttcggtccg
     8761 ctaacagtat gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt
     8821 gttagagatt caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa
     8881 aaaaccatcc cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca
     8941 aaatacaatg ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta
     9001 aaaaaaatcg agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca
     9061 aattttaaaa aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt
     9121 actaaaggag aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc
     9181 tttttggaag agttagacaa agcatcagct gaagctggaa tcacccttcc gtcaggagct
     9241 ggaacgaaat ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt
     9301 gaaaaatatg ccaaacttca ttttgccaat acagaaaaag ctttgaagct actaaaaaaa
     9361 taatggaact gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttcaat
     9421 catctgatcc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     9481 gtttctctaa tactcagaac aaaacgttga tttcacaacc tttttcttaa acggtgttct
     9541 catgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga
     9601 aattgctcct tgtgactcag agttgaacgg caggctcacg acctcttcct taaacggtgt
     9661 ttaaaagctt gcatctgcga taataagccc ggccaaacga aaaatatgag gagctatttt
     9721 tgtttggctg ggcttattta ttactgaaaa taagtggtca atgcttgtgt caaacttgca
     9781 gcaatctgct gctgataact gtctgttact aattccttta aatcactatc attattcata
     9841 taacccatct cgattaaaat agaaggttga agattttctc gaagaacttg atagttccca
     9901 gtggagattc ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat tgtctgtgct
     9961 aatgtttcct cttgtccata gtaataatat gtcgtgattc cagttgcttc attcgcatat
    10021 tccgtagagt ctgcgtgtaa actgataaag acatctgcct ttttttcttc actgtagtcg
    10081 catatatctc caagctgtac taattcatct gtagtatggg tcaaataaac cgttgctcca
    10141 gcatcctcta gcgccttttt tacttttatg gctgtactta atgttatatc tttttcttct
    10201 gtttgttcat cattgcttaa tgccccagta tcatttccgc catgcccagg atctaataca
    10261 atcactgcat cagatagctc ttccggtttt gtttgtatat tctttgtgac tttggttcca
    10321 tcttcgaggg ctaaatatcc agtatagcca tcatttgtct tgatctgata agcatttttt
    10381 gtttcagaaa gaaactccgc cagctctccg ccgtttatag ttgcaagtgg ggtagttagc
    10441 gagtcatctg tatatagtgt acggtcattt tttccgattt tgattaatgt ctcttgttct
    10501 gtatcatcaa atgattcctt tgtctttgta accgtactgt tcgtactgct gaccgtttgt
    10561 ccaactatct ttttttgacc aaaaacatct atttgaaaac agataataat gataatgatt
    10621 gcaaatacaa aagctccaat tatttttttc attcttgact ctcacctcgt agatccagac
    10681 taagggccaa aacttaaaat cagcttaagt aagcaaaaaa ggggctgtaa aagatataac
    10741 cgtagaaaat aacattactg ctaaaatgat ttttttcatt tttggcatat cctgaaatct
    10801 ccttttgact attcgtggtg attcatctac ccaataaata gtttccttca ttctacaaac
    10861 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
    10921 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
    10981 aagaagcaag atataacgat cttcgtcata tcttgcttct tcacctaact attttctccc
    11041 tatataacag ctttacgctt ctcctaatac accattcaaa tataaatcga tcaattgcgc
    11101 cacatttagc ggttcttcca caggtctttt cgaccgagtg atgtaaggtg ctaacactaa
    11161 gaaaaaatgc cttgctgcga tttcttttga aatcccattt cttagcacag attggtttgc
    11221 tgaaaagatt tcttcaaaag gttcacgata atttttcttc catagaaaga aaagatgatc
    11281 tcttgtttcc tttgaaagat tgctttcaag atcgtgaagc atcagaaaca aatcgaccag
    11341 atgtgtctca atcaaaaact gcgccatttt tgtcaaggtt acttgaaatc ccgtttcgct
    11401 tgtttgaacg atttctctca aagcttttcc aacatctttt aagtggtttt ctaaaactgc
    11461 cgtatatagt ttttctttat caccgaaata atgatagaga ttgggctggg tgatcccaac
    11521 tttttgtgcg atttctcttg ttgaagtgcc gagatatccc ttggacataa acaagtcagt
    11581 cgcttctttc aaaatttttt gctgtgtatc tttgttgtat cgatggacca tttttccacc
    11641 tgctttttgg ttattctttt tctttcaata caccatcttc catcataaat attctatcgc
    11701 atttgtcaat cagccgtgta tcgtgagtaa ccatgatcgt tgctttattt ttgtcttttg
    11761 attcttttgc caagatatcg actacttctc gtgccttttc tgaatccaaa ctagcagttg
    11821 gttcatctgc tagaataatg gacggatcat tgtacaatgc acgagcgatt gcgacccttt
    11881 gccgttctcc accagatagt tcttctggat acttgttgat caacttatcc acgccaagct
    11941 gctcaaataa ttcgtttgct tctttttgac gatgttcatg tttaatctta tctactaata
    12001 ataactgctt ttttactgtc agaaaaggaa ccagattgga agcttgcaaa ataaagccaa
    12061 tttctttaaa acgtagttta acgcgttcgc tttctttttg attactgaat tcttgatcat
    12121 tgataaatac agagccactg ctaggcgtct gcaatccgcc tgccaaagtc aaaaaagtac
    12181 ttttacctga accagaagga ccgatgattg caacaaattc ccctttatcg atactcatgg
    12241 ttgtttcttt caatgcttta actacttggt cgccatcttg gaaacttttt tctacttctt
    12301 taaattctat tgctttcatg ttcttcctcc ttcttatcca attgccttca acggatcgat
    12361 ttttacgatt gtccgaacag aaaatagtgc gcctaacaca gctactacta acatcaacaa
    12421 gctaatgacg ccaaaaaaca gccaattgct ttggaatgga accgcatcag gtagaacaag
    12481 ggatgttccc actgttccta atagaccaag aagaatacca acaaatgata aaatgaatgt
    12541 ttgtgccact acagaacgcg caataaatcc tcctgtgatc ccttgtgctt tcattacacc
    12601 aaagatatta atcttttgca tcgtcagtac ataaatgaaa atcccgatca caatagccgc
    12661 aataacgatc aaaaatccga tcataaatcc aaaagtcaat acttgcgcat tatatccagg
    12721 taattcattg atgaatttag aaattttgat ctgttctaaa tcttcaggta aattactgat
    12781 tttacctctg acaactattg cgttaatccg tgcattttca cttgtatctt ctttttcgaa
    12841 gcggatttct tggtaagcat tgatggttgt atagagaact ggtgagacat tgaatttggc
    12901 atgatccgta aagccagtta gtttaaacgt cttgtcacta ccagataact taactgtatc
    12961 tcctatggct agaccatatt cttctttcaa actaatatcg cctactgcct catcatcgtt
    13021 atcaaaagct tttccttcaa caagattggg catgatgaat tgatttttat cgataccgaa
    13081 gaagctaaca ttgatttttc cttcttctgt actgtcttta ctagtcacga ctccaggtgt
    13141 ttgggcaaga tacgccactt tcccaccttc gacttcttct gctatcttct tagtaatcat
    13201 ggacatccct agatttgaat ttgcatcttt cgaaagcaca atcgaatctg cctcccactt
    13261 gtcgactgct gttcgattat cttgagctaa gccataagct aacccagtca aaaaaaatac
    13321 caggtaggcg atcaaaaaca tcacccctgc aactaacgca taacgtaact ttgaatgcat
    13381 aatttcattt aatgctaaaa acatttaact cctcctcctg aaataatcat tttatacaac
    13441 aattttatca cttgataaat ttttggcgaa tattatgctc aaaaaattct tccgctattt
    13501 cattatatga attttttatt ctcgattgat taatatttat tatatcccca cacaataaaa
    13561 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
    13621 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtattcaga cagaaatcca
    13681 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
    13741 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
    13801 tccggatggt agatcagctt tgcatttact gttttgtttt cttcgtcgta ttgtggaaat
    13861 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
    13921 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
    13981 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
    14041 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
    14101 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
    14161 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
    14221 gcagaaaaaa tgaccatatc tgcctttatt gtttgagaca gaagttgtac ggatactact
    14281 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt tatttgctta
    14341 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
    14401 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
    14461 tccaatccaa tgtaaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
    14521 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
    14581 ttatacaagg tgacaggaga ggaacctgtc gctacgatta atttatcata ggttatggtc
    14641 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
    14701 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
    14761 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
    14821 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
    14881 cttagattta gggcagctgt gatgccttca tgggaagcac caatgatcac gattttcatg
    14941 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
    15001 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
    15061 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
    15121 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
    15181 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
    15241 tgatcagcgt ccgtaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
    15301 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
    15361 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
    15421 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
    15481 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
    15541 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
    15601 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
    15661 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
    15721 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
    15781 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
    15841 atttcaatcg tccttcttct gtaatagcaa aactaaactc tttttgcagc agatctaaaa
    15901 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataggga cgtccaaacc
    15961 aaaataattg gaaataaaaa taacgaattt gactttctgg accggtcagc cgcccgcttt
    16021 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
    16081 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
    16141 acagataaag aagtatctga tattttgtag aatagcgcaa gtaacaatga taataaacat
    16201 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
    16261 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
    16321 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aacctctcct ttttcctcta
    16381 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
    16441 ccaagtccac tccttcaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
    16501 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
    16561 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
    16621 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
    16681 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgc
    16741 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
    16801 ttaaataaag aagtcaatgt acctgtcagt tgccaaggag acgtgcttgg aagccgagta
    16861 tcccgcacat caaaagaaaa atcgcctttt gattcataat aacttgtctt caacggtttg
    16921 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
    16981 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
    17041 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaaa
    17101 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
    17161 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
    17221 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
    17281 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
    17341 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
    17401 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
    17461 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
    17521 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
    17581 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
    17641 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
    17701 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
    17761 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
    17821 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
    17881 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
    17941 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
    18001 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
    18061 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
    18121 gttgaatcac ttggtgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
    18181 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
    18241 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
    18301 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
    18361 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
    18421 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
    18481 tctgtcaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
    18541 gctgataata aacttccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
    18601 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
    18661 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
    18721 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
    18781 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
    18841 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
    18901 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
    18961 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
    19021 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
    19081 ccataattct ataccaatga tcaaataaag aaaacggatc ttttgttcat tcgtctccgg
    19141 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
    19201 gccattgact gaaccacttg gatgcggaat tggacttaga tcataaaatg taatcgtacc
    19261 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
    19321 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
    19381 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
    19441 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
    19501 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
    19561 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
    19621 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat gcattagacg
    19681 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
    19741 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
    19801 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
    19861 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
    19921 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
    19981 ttgctgggat tttcacttct ataggtacag ttgtttctgc atgtgctgat acattgattt
    20041 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
    20101 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
    20161 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
    20221 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
    20281 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
    20341 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
    20401 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
    20461 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
    20521 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
    20581 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
    20641 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
    20701 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
    20761 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
    20821 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
    20881 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcgggaac
    20941 tacatccagt gttaaaggac ctgtctggcc agttgctgga ttttccggat ctgctggctc
    21001 tactggtttt gaaggatcag tagggttaag aggctccacg gtatttgttg gttcagtaaa
    21061 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
    21121 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
    21181 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
    21241 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
    21301 acatgaatat tcttcctttt ttattaagtt tcgtttgtca aattaagtta gacaaatcat
    21361 cttttgtgac gtaactcaaa aatacttcca ttggtgtccg atagtgtaat gattttctag
    21421 gaatatgatt tcgtttcgat gccaccgatt ggataaaggt ttcagataca ccattaaaat
    21481 ccatagcttt atacaaacca tctttacgta ataagccatt tgaatgttca ttcaatcctc
    21541 gttgagaagg tgttcctgga tccgcaaaat aaattgaaat atcgttggta ttactaatct
    21601 ttttccaatt tgagaattct tttccacaat caaatgtgat ggatttaaat aagttttttg
    21661 ggacacaacg aaaccattga ttcagcctat tttcaatgtc tttagcctgt ctgccttcgg
    21721 gtttcaaggt aatgatgact ttagatagcc gttcaactaa tgtgatgacc gcgcttttat
    21781 gttttccacc cacgatagtg tcaccttcca ggtgaccaaa ttcatgatca aactgggtat
    21841 agtctgtctt tctgtctcgg attgaccggc gaaacccttg ttttcctctt ttttctttat
    21901 gaccgtttgg ttttcttttt cccttcattg gtaaatcgtg agtgtcaaag gttttatctt
    21961 taaacattcg atacagtgtg cgtaccgaac aagcaatagg aaactctgct cgaccaataa
    22021 ggacatcagg agcccaccct tgaacaacct ttttttgaat gtattcctgt tgctcatcag
    22081 gtaaaacaat cggatgtctt ccgcagttac gtttgttttt cttatattgt ttatagtagc
    22141 tcattgcgct tccaccagat ttaagaaaac gatagacctt ataaatagtt tgtcgagaac
    22201 gttgtaacag attggctact tttgaaacag gttgtgattg attgaaataa gcttctatca
    22261 ttacgagttc agttggtgta agatggttat aggtcatttg tactcactcc ttataattct
    22321 ttcgtcggaa ctattttgag tgtagcacaa atgacttttt ttagttgtct agcttaattt
    22381 tacaatcggc gaagtatgaa aaaatatttc aattttattt tttcgtttca aattattcta
    22441 ttagtgacat ccttgaacta taaaaatagg agtacggcat catctgccgc actcctattt
    22501 ttttcgtcat ttgtttctta aataaaacga ttctcacgca attttatttt tttgatatct
    22561 tctactgttt gtgttccagc taattgcatg accatttcca attctgtttt aaagaaatca
    22621 aacacttgat ggactcctgt acttcctcct aaagacaatc cataaataac gggtctgcca
    22681 atagcaacaa gatctgcacc tgatgcgatt gctttgaaaa catgctgtcc gcgtcgtaca
    22741 ccgctgtcaa acacgattgg tacacgttta tctactgctt ctgccacata ttgcaacgaa
    22801 tcaaacgctg ctggaccacc atctagttga cggccaccat gattagatac ccagattccg
    22861 ccagcaccag attctaatga acgatagaca tcttcttctg actggacacc tttcacatag
    22921 actggcaaat cagaatgagc agcaataaat tctacatctt ttggactaag cttctgtttg
    22981 gaagatttgt acacagcatc catcgtttgg cctactcctg attgataagc ctgaacgatc
    23041 ggcattggca gtgggaaggt aaacccattt ctgcggtctg tttcacggtt tccgccaact
    23101 gttgcatccg ctgtcaatac gattgctttt gctccattac gcttggccac ttcaagaata
    23161 tccaaattga tcccatcatc tttgctcata taaaattgaa accattgcgg tgctttctcc
    23221 ccgcctgctt ctcttatctc ttctaatgta caagaagcgt aagaacttgc tgtatagatc
    23281 gtcccaaaat ctgctacacc ttttgcagaa gctttctccg ccttcacatg agctaagcca
    23341 tgagcagcaa ctggtgccat gatgattggt gcagtcagcg tttctttgtc aaaatgtgta
    23401 gttgtatcgg gtaattctac atctcttaat acatgaggaa taatcagctg atgattgaat
    23461 gcccgttcat tttcttgata agtaaataag tcgccagcac cactgctgat gtaaccataa
    23521 ccaccagctg gaatcacttg tgaagcggct atttctaaat cttccatatt gataaaatcc
    23581 actattcctt catttgtacc tgcttgatac gttttttcca tttcaatgcc ttccctctat
    23641 aaaaaattga atgaataata tgatagcgtt tacacacttt atattactct tccgtattga
    23701 tacaggcaaa taatctgttt acatttcagt cattccagtt gttactactc tgaaagaaat
    23761 gtttagaaca caaatagata aaaaacgatg ggattttgac aaaagtcgtg tcaaagtccc
    23821 ttttacgaaa tactgtggat gcctattgaa ataagtgcgg gttgtttctt tgtatttcaa
    23881 tcgtttcttg ccattcttcg tttaattttg gattaccgtt ttcttccttg aacgcataag
    23941 gtcccaatcc aaaaagctca gttagtattt gattcacgat tccatatagc tcggtgtgat
    24001 cggacacatg attattgaag atcatctcat tatcaccacg tttgaatctc acattcgggc
    24061 tctcatgatg aacctctatg cagttttccc ctttttttaa ctgacggatt tctgaaaaca
    24121 tctcataaat atcttctcca ataaaacctt cagcaagcgc cagactgtcc acaagaccgc
    24181 ctactgtcaa ctcagagatt atcgctttgt tggcaccaag ctttaatgta tagcttgttg
    24241 cttctactac gccgcatgca ttgcaaccac cttctgatag ttcgcttttt ctgatcaatt
    24301 gaacttcttt cattttctgt tcctcctctt agttttctac ttatgtattt tttatcacct
    24361 gacgtaagtg tattttatcg tcacgatcac gtgatttatt cttcagagat tc
//
LOCUS       DAJSNV010000037        23703 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSNV010000037 DAJSNV010000000
VERSION     DAJSNV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.038"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ON516_001388"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ON516_001388"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3344880.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ON516_001389"
     CDS             complement(1173..1868)
                     /locus_tag="ON516_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3344881.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ON516_001390"
     CDS             complement(1881..2597)
                     /locus_tag="ON516_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3344882.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="ON516_001391"
     CDS             2927..3535
                     /locus_tag="ON516_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3344883.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3860..4183
                     /locus_tag="ON516_001392"
     CDS             3860..4183
                     /locus_tag="ON516_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3344884.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4365..5735
                     /locus_tag="ON516_001393"
     CDS             4365..5735
                     /locus_tag="ON516_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3344885.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5865..7166)
                     /locus_tag="ON516_001394"
     CDS             complement(5865..7166)
                     /locus_tag="ON516_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3344886.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7520..9478)
                     /locus_tag="ON516_001395"
     CDS             complement(7520..9478)
                     /locus_tag="ON516_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3344887.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9655..9921)
                     /locus_tag="ON516_001396"
     CDS             complement(9655..9921)
                     /locus_tag="ON516_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3344888.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="ON516_001397"
     CDS             complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="ON516_001397"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3344889.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="ON516_001398"
     CDS             complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="ON516_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3344890.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13265..13828
                     /locus_tag="ON516_001399"
     CDS             13265..13828
                     /locus_tag="ON516_001399"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3344891.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14081..14602)
                     /locus_tag="ON516_001400"
     CDS             complement(14081..14602)
                     /locus_tag="ON516_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3344892.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14605..15447)
                     /locus_tag="ON516_001401"
     CDS             complement(14605..15447)
                     /locus_tag="ON516_001401"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3344893.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15440..16165)
                     /locus_tag="ON516_001402"
     CDS             complement(15440..16165)
                     /locus_tag="ON516_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3344894.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16341..16637
                     /locus_tag="ON516_001403"
     CDS             16341..16637
                     /locus_tag="ON516_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344895.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17205..18797
                     /locus_tag="ON516_001404"
     CDS             17205..18797
                     /locus_tag="ON516_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3344896.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18810..19550
                     /locus_tag="ON516_001405"
     CDS             18810..19550
                     /locus_tag="ON516_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3344897.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19718..21067
                     /locus_tag="ON516_001406"
     CDS             19718..21067
                     /locus_tag="ON516_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3344898.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21195..21785)
                     /locus_tag="ON516_001407"
     CDS             complement(21195..21785)
                     /locus_tag="ON516_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3344899.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21932..23284
                     /gene="brnQ"
                     /locus_tag="ON516_001408"
     CDS             21932..23284
                     /gene="brnQ"
                     /locus_tag="ON516_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3344900.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6797 a         4773 c         4511 g         7622 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcaaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc ttttttattt
     3601 ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca aacgaaaaat
     3661 cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa atttatactt
     3721 tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga atgaaccctc
     3781 gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa cattcgttga
     3841 ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc gttacgcttc
     3901 agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt ttattattga
     3961 cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt tgatcgaaaa
     4021 tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa tggatattga
     4081 tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg atgacggtac
     4141 gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata gataaacgtg
     4201 gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa ttcttggctt
     4261 ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca ggataaacgc
     4321 tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc gatttaacaa
     4381 aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc ggaaatgttt
     4441 ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta ggtaaaaatg
     4501 cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc tttgcccaag
     4561 gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa gattatcggg
     4621 gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact gtgattttaa
     4681 ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact cctcctgaaa
     4741 tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt gcatcagtca
     4801 gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca ccactattct
     4861 ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt atctattttg
     4921 gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct tccagcgtac
     4981 tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa aaagatttca
     5041 aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg gcgtttcaat
     5101 cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt ctagggacag
     5161 atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat cagccgatga
     5221 tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg aaagaatatg
     5281 gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt agtctcttag
     5341 caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt agcccatcag
     5401 aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct ctttactgga
     5461 tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa agcaagatcc
     5521 ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc gttcttacag
     5581 gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc ggttctgttg
     5641 ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg ggtgaagagg
     5701 aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa atcgttgcgg
     5761 ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca ggttttttag
     5821 ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc tgtttttctt
     5881 tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa taccgtgaaa
     5941 cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg gctagaagca
     6001 aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac atgaaaataa
     6061 cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc tgactgttcc
     6121 gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat ttcttctaaa
     6181 atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa gatagcaata
     6241 tgcttaccct tttttcgcat gatcgtcaat aattttttta tagggatcgt ttcagttgtt
     6301 acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata agattgcttg
     6361 atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa gataaccgga
     6421 tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt ttctgaagct
     6481 tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt atcatcaaat
     6541 tcaaacacgt tttccaaata ataatattct tctttggtca actctccttt ttcataagaa
     6601 ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc agatacgaga
     6661 cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca aataaaagga
     6721 aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac cttctccgta
     6781 ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat atacgtaact
     6841 aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa aggtagttta
     6901 ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc gatgatcaaa
     6961 ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg gtgtgtaaca
     7021 tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa tcttgatcgt
     7081 ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc aatcatcaaa
     7141 aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct attgtaccct
     7201 ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa aaggagatgc
     7261 ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt gttgctaaac
     7321 agctttttca tgacctctga taaaaacggt attcaaaagc ttgcatctac ggcattaagc
     7381 ccaaacagat gaaaaacatg agaagctgtt tttatctgct cgttcttact gcttgttgca
     7441 gtacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7501 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7561 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7621 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7681 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7741 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7801 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7861 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7921 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7981 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     8041 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     8101 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     8161 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     8221 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     8281 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     8341 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     8401 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8461 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8521 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8581 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8641 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8701 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8761 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8821 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8881 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8941 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     9001 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     9061 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     9121 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     9181 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     9241 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     9301 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     9361 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9421 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagccattg
     9481 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9541 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9601 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9661 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9721 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9781 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9841 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9901 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9961 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
    10021 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaatcaga
    10081 ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    10141 atattttgcc gttttttgag ctaatatcga gggacagctg attgaacatc gtttattgga
    10201 gaggttgtaa aaaagctgtt ctgcatcaag taataagaac agccaaataa aaacagctcc
    10261 taatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10321 tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc ccttttttcg aataattatt
    10381 gtccccctgt caccttcacg atcacaagta cattattcct atgaattaaa ttatttttct
    10441 tcatttttcg ttgaaccatc ttcgttcaac attgcttttc ttgacagatt tacgcgtcct
    10501 tgtttatcga tttcagttac tttcacaagg atctcatcac ctagtttaac aacatcttcg
    10561 actttattga cacgatcgtt tgctaattgg gaaatatgga caagtccatc ttttcctttg
    10621 atcaagttga cgaatgcacc gaatttttcg atacggacaa ctttccctag ataaacttca
    10681 ccaactttga cttctttggt caaatcttcg atgatcttga tagctttttt gatcatttct
    10741 gcatcagctg aagcaatact tacttttcca tcttgatcga tatcgatttt cacaccagtt
    10801 tcatcgatga ttccgttgat cgtatcgccg cctttaccga taacatcttt gattttagca
    10861 ggttcgatct ggatcatttc gatttttggt gcatattggc ttaattcttc acgtggagca
    10921 gcaatcgttg aagtcaattc ttctaagatt tccatacgtg cttgttttgc ttgagttaat
    10981 gcttcagtta ggatttgttc tgtgattcct tgaatcttga tatccatctg taaagcagtg
    11041 ataccatctt ttgtaccggc aactttaaag tccatatcgc caagatgatc ttccagccct
    11101 tggatatcag ttaggatcgt atagttttca ccatcagaaa caagtcccat cgcaataccg
    11161 gcgactggcg ctttgattgg tacacctgca tccatcaatg ctaatgtgcc agcacagata
    11221 cttgcttggg aagaagaacc atttgattcc aatacttctg ctactaaacg gatcgtataa
    11281 ggaaattctt cttctgaagg gattacttgt gctaacgcac gttcacctaa tgcgccatgt
    11341 ccgatttccc gacgccctgg agagcctgca cgtcctgtag aaccaacaga aaattgaggg
    11401 aaattgtagt ggtggatgaa tcgtttgctg acttccaccc ctaaaccatc aatgatttgg
    11461 tgctctccta gaggtgcgag tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac
    11521 aaacctgatc catgtacacg tggcaataag ctgacctctg atgacaatgg acggatttca
    11581 tccaatttac gcccgtcagg acggatctta tcgatggtga tcaattcacg tacgacatct
    11641 ttttcaagat cttctgtgat ttgtttaact tctttcaaca agtgatcttc atcttcatga
    11701 cctgcaaatt tctcagcata agcttcgcgg atatcgatct tcacctgctc gatattatct
    11761 tcgcgagcaa gtttttcttc agtcattact gctgctttca ttgtagcgta agaagcatca
    11821 aagatttctt ttttcaatac ttcatcaact tgcagtaaag tcacttccat tttttctttc
    11881 cctactgctt gaacgatttc ttcttggaaa gcaactaact ctttgatagc gtcaaaacca
    11941 aacagcaatg caccaagcat gtcttcttct gaaacttctt tcgcgccgct ttcaaccata
    12001 ttgatggctt gtttcgttcc ggcaacagtc aattcgatat ctgtttgttc tgcttgttct
    12061 actgttggat tcaagacgta ctcgccattt acacgaccaa cttctacgcc tgcgattggg
    12121 ccgtcaaatg ggatatctga gattgatagt gctaaagaag aacctagcat tgctgccata
    12181 gctggcgagc agtctgtttc tacactcatc acgatgttcg ttacttggac ttcattacgg
    12241 aagccttctg caaacatcgg gcgaattgga cggtcgatca aacgggcagt caatgttgct
    12301 tccgtacttg gacggccttc tcgtttgatg aaacctccag ggattttccc tgctgcatac
    12361 attttttctt cataattgat cgttaatggg aaaaagtctg tatcttttgc ttctttagag
    12421 gccacagctg cacttaagac tactgtatct ccataacgga ctaaaacggc tccgtttgct
    12481 tgttttgcta gctgaccgac ttcgatttgt aagggacggc cgccccatgt cgttttgaat
    12541 acttgttttt ctgacataac ttctcctttt ccttctcgtg aaagcaagac gctactaaac
    12601 aaatgaaaac tgccgaaagc cgtcttttga gcagctttca tttggttagt aggagtccat
    12661 ctagcttgat cacggaattt tacttaattg atatacgagt aaaaaaagtg agattcatgt
    12721 gaatcccact ctttttgact aaaattaacg acgcaagcct aaacgtttga tcaattcgcg
    12781 gtaacgttga acgtcagttt tacgtaagta agctaacaag ttacggcggt gaccaacttt
    12841 tttcattagg ccgcggtatg aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa
    12901 gtggttgatt tcttcagtta atacagcgat ttgtacttct ggagaaccag tatccccttc
    12961 atggcgagcg tattcgttga tgatttcatt cttgcgttct tttgaaattg ccatttcttt
    13021 cacctctttc gattattccc ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt
    13081 ggcaggtcat gtgcctagag cacacagcta tactttacct gaaaccgggt tgaaaatcaa
    13141 gtagttaatc attaagaaaa ggtagttttc aaacaatctt actactactt ttccattttt
    13201 gtggtaaact ataatgcgaa aatagacttg aaacacagtc ttttaagaaa ggggtacata
    13261 ttccatgatt acaatggatg atatcattcg tgaaggaaat ccgaccttac gcgaagttgc
    13321 aaaagaagtt tctcttccat taagcgaaga agatatctct ttaggaaaag agatgcttga
    13381 gttcttaaaa aacagccaag atccaatcaa agctgaagaa ctacacttac gaggcggtgt
    13441 tggcttagct gctcctcaat tagatatttc taaaagaatc attgctgttc acgtaccaag
    13501 tcctgaccca gaggcagatg gaccttctct aagtactgtt atgtataatc ctaaaatttt
    13561 gagccattct gttcaagatg cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg
    13621 agaagtacca ggatatgttg ttcgccatgc caaaatcact gtgtcttatt atgatatgaa
    13681 tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat
    13741 cgaccatatc aacggtgtga tgttctatga ccacatcaac gatcaaaatc catttgcact
    13801 aaaagaaggc gtattggtga tcgagtagag aattggcagg ttccttcgat actatgctac
    13861 aagaaagctt tataaaaaag gggagtgctc aaaagctgca cttccctttt ttatgtttct
    13921 ccactaatcg aaatttagat agacatctag aaatcgcaat tccaaaaaat tctgtgtatt
    13981 ggatacgcat cttttcttat tttttcttat ttcttccata tgggtgttta tctcagagat
    14041 gatagaatag tttctaggat aaaaagtagc agctacgatg ttatccttcg tagaggattc
    14101 ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg cttttctggg tagcggatcg
    14161 tatgcagatg gatgccgcct gttaattcgg ataacaaagt agcattactt ttttgataac
    14221 gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg caaagttcct gctaactcac
    14281 cgtaaagggg atgttccaca attacatcaa tgatttcacc accattttca acgattagtc
    14341 tcagttcttc ttctgtttga tcttttccat gctggacagc gattttactg atgtgcccag
    14401 tattggtttc ttctgtttcg aggatataac ctcttgctgt tgccacgata cttttacctg
    14461 cagctcgtag taaagcaata tcgcctacaa tgatctgacg gctgactcca aaagttttcg
    14521 caaacttgct agcactcact ggtttttctg tatttgccaa ggtttgcatg atttgttttc
    14581 gccgtgtctc tccttcgatc acacttattc ctccttcatt ttgttttggt attcgcgcga
    14641 gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc ttatatccct tatttttcag
    14701 cagactaaca actttatcca atacttgctg ctctcttttt gcatcataaa ctgcttggtt
    14761 ttcttctttt ttcacttgtc ctattttagt taccgtatcc attctttttt ctaataaagc
    14821 aaccaactct cgatcgatca tatcgatctt atgacgtaat gtttcaagag actcttctgt
    14881 tagttcttcc tctactgtct tgatgatacg agcaggattt cctgcaatca ctacattgtc
    14941 gggaaaagat ttggtcacaa ccgaacctgc gccgactact acgttgtccc ctagagtgac
    15001 acccggcgtg acgataactc ctccgcctaa ccaaacatta ttaccgattt taatgggttt
    15061 tgcgtactcc agaccgctgt tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag
    15121 ctggacattc ggagcgaaca tgcagttatc accgatttca atcgtgctga catctaaaaa
    15181 tgtacaattg aaatttgcat aaaaattctc cccaacaaat atattatatc cgtaatcaaa
    15241 attgataact ggctccatat agattttttt ccctgtacgg ccaaaagtct ctttcaacaa
    15301 ttgggatcgc agttccgctg attctgcttg gttgatgatt tggctttgac ttcttgcaaa
    15361 catacggtcg gcaaccagct caggatcatt ggcaaaataa agctcgcctg caatcatttt
    15421 ttctttttcg gttttgaatt cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa
    15481 caaccgctcc gtcttcttga ataaacgtat aaggctccgc taaataaaca ggcgtttgat
    15541 tgattactaa gattttggct gacggctggc gaaactgtac cagatcacaa aacggatgaa
    15601 cttgaaaact cgttccaaca atgacgatca gctctgcttg gctgacggca taagccgcct
    15661 gctccatgac tccatcttga aacccttcac catataacac gatgtctggg cgtatttgtc
    15721 ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt ccagtctacc gtttgttgac
    15781 attttcgaca atgacattga tacagactcc catgaaaatt gacgagttgc gggcttttag
    15841 cctttgcgtg caaaccgtcg atgttttggg aaacaaccca aacatttttt tctttcgcta
    15901 gatgtgctat ttccaaatga atgatattgg gttttgcttc aggatgataa atctttttga
    15961 caaattgata gaatttatcc ggttcattca ccattgcttg atgactcagc aagtattccg
    16021 gctgctccat cccgtggtag attcctgaca aagatcgata gtctggcact ccagaaggtg
    16081 tggatacccc tgcaccggtc aaaaatgtaa tttgctgata agctaatatc tcttttgctg
    16141 cttcgtctac tgttaattgt ttcatgtttt gtcacctcac tttccattat agcccatact
    16201 cgaacaaatt tattgttgaa aagtcagaaa aatgggaata aaaaagtgtt atgggcattt
    16261 gagcaattta tcacaatgtt cactgacaaa agaatgaaaa cgtgttacaa tactgctgta
    16321 taaaagtgag gtgaatcgat atgttaccat tctttattta ttggggcgtt attctggctt
    16381 gtatcgcctg gttagcgtta agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa
    16441 atggaaatct ttgggctttt gctttattca acgtgattgc cgcaattgtt ttagccatta
    16501 cattagccgt atatcgaaca tggggctggg gaatcaccca atactcaagt ttgatttatc
    16561 taatattagc catttatggc gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa
    16621 agaaagcggc agcttaaatg atcgagatgg aactggacac aggaaatttt ctgttctagt
    16681 tcttttttta ttgtattaca tacatttttg tgtataatta tactatcgtt tgcaatggat
    16741 agatgattca cattttgatt gttttttcgg taaaagtaaa gatttctata tttatcagta
    16801 ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt aaactttttt
    16861 tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc gagaatcatt
    16921 aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc
    16981 acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac tgtcttagca
    17041 gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa ttttagcggc
    17101 ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt accgcatttc
    17161 atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa aaaaatatgg
    17221 tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga tctctggcaa
    17281 atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct gggtatgcac
    17341 ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa ggccaaaata
    17401 gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa
    17461 aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga
    17521 tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa attgatttta
    17581 ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc aaatatgcaa
    17641 aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta aatacattcc
    17701 actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg gataatttct
    17761 cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg
    17821 aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc agcggtgcag
    17881 atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt aaaggcgacc
    17941 cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa cgaaccaaaa
    18001 ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa aaacctggca
    18061 tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta cgaggtgctg
    18121 gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt ggattactga
    18181 ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa
    18241 tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg atgatggtac
    18301 aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg aaccgaacga
    18361 ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag atcgttcgtg
    18421 gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc
    18481 atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc ttgccagcaa
    18541 caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta atcggcgtag
    18601 ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc ttccaaacat
    18661 ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga atcctgcggg
    18721 cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa atcgacaatt
    18781 caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa atcaaacatc
    18841 taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca gtcaataaag
    18901 gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc ttgcgctgta
    18961 tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa aatgtattag
    19021 ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc caatccttca
    19081 acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca actgtcctga
    19141 aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg
    19201 atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa
    19261 ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct acttcggcgt
    19321 tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca catactggct
    19381 tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct gaccgtgtca
    19441 tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc tttgtccatc
    19501 cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aaatgcttag gatcaatagc
    19561 tgattgggac aaagagctgc ggtgtttacc gcagttcttt tttatgaatt actcataaaa
    19621 ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt cagaaaactc tcttaatatg
    19681 gaagtaatta aactttttta gttaggatga tttaaaaatg aatgaagagc aagaactgca
    19741 gcgtggactg aaaaatagac atgtccaact gatttctatt ggcggagcga ttggaacggg
    19801 gttgttttta ggttccggaa agtctatcca gctagctggc ccttcgatat tgctcgccta
    19861 tcttattact ggatgcatct gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc
    19921 aaacaccaat aaccattctt ttctggattt tgttgcagaa tatcttggca aaaaagcggc
    19981 tttcatcact ggctggacgt attggttctg ctgggtatcg attgcaatgg ccgatttaac
    20041 agccatcgga atgtatgtac gctactgggc gcctggtgtt cctcagtggc ttcctgaact
    20101 gattgcatta ggtcttcttc tttgcttgaa tatggtggct gtctcattgt ttggtgagtt
    20161 agagttttgg tttgcattga tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc
    20221 ttatatgatc ctcacccact atacgacaga tattggcaca gccagtatca cgaatctatg
    20281 gtctcatggc ggattctttc caactggtgc aaaaggattt atcttagctt tccaaatggt
    20341 aacctttgct tttgctggga tagaattagt tggattagtc gctggagaaa cagaaaatcc
    20401 tgaaaaagtg ttgccggaag ctatcaataa tattcctatc aggatcatct tattttactt
    20461 aggatctctt tttgtcatta tggcaatcta tccatggaac agcttgaatc cagataacag
    20521 tccatttgta gaagtatttt cggaaattgg gatcacgatt gctgcttcct tgatcaattt
    20581 agtcgtatta agtgcagcag cttctgcctg caacagtgca atatatagta caggacgtat
    20641 gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca
    20701 tcatgtgcca gggaatgctt tgacattttc tactatcgtc attttcattt ctgttatctt
    20761 gaattacgtg atgcctagtg aagtattcac actagtaagc agtattgcca ctacttgttt
    20821 cttgtttatc tggagtatgc ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg
    20881 caaaaaagct cattcattta aaatgccgtt gtacccattt agtaattatc tggtatttct
    20941 ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt
    21001 actaacacct gtgtggttct tgcttctatt actaatctat catatgaaat atgaacgtaa
    21061 gaagtaagaa agtagttgtg acagaagtgg tcagcttcga gaaataagaa ggaatttacg
    21121 aaaattgctt cttaaatttt tgtgaaattt cggcttattt cttggagata aaagcttctg
    21181 tcccagcctc ttttctattt ccccggaaac aaccgctgat aataggcatc tgctttttta
    21241 taggaaaaaa tccaaatacc agatacaaaa aaacgtccga ataacccgat caacggtaca
    21301 aaaaagacac cggcaatcaa gcataagtta atcatcatgg agataagact tcgacggtcc
    21361 tttgacaaaa ctctcacgac atgggaatct ggtttatttg ctttcatgat ttgcctcatc
    21421 aagataccaa aagaaaaact ccacatcgtg tatactaaga cataagccat ctcaggccaa
    21481 aaagcttcag ggttacgccc gacccaacta gtaacagaag gacataaagt cagccaaaag
    21541 agcaaaaaga tattcgacca gaccactcgg taattaattc gttctgccat ctgaaatata
    21601 cgatgatggt tcacccagat caccgcaaca aatataaagc tgatggtata agctaataat
    21661 gtatttcgga tgacccataa agaatgaaag ctagatgtat tagggatctt gatctctaat
    21721 acaagaactc tcaaaacaat ggctaccact ccatcagtaa atgcttctaa tctcgacttt
    21781 gtcacgatga ttcctcctca gatgattcag cgcttttatt ttatcactta cgttattaaa
    21841 taaccatcgt tttatatagg gaattttctg aaaattgttg catatttttc gaaattttgt
    21901 tacttttaaa aaaaccgtaa aggagttatg tatgacaaaa aaattatcat ttaaagacta
    21961 tctttttata ggctccatgc ttttcggtct attttttggt gctggaaatc ttatctttcc
    22021 cgttcatttg ggtcaagaag caggcgctgc taccttttgg gcgaatctag gtttcttggt
    22081 taccggtatc ggtcttccct tcttaggggt gattgccatt ggtgtatcga aaagctcagg
    22141 tgtatatgaa ttagcaaaac ggataaatca aacatatgcg atggttttta ccatcttgct
    22201 ttacttagtt atcggaccgt tttttgcttt acctcgcctt gcaaccacat ctttcgagat
    22261 tggtttagca ccatttatcg atcctagcag acagacgcta tatctagcaa ttttcagtat
    22321 tgtatttttt gtgctggctt ggtggttttc aagaaaaccg acaaagatat tagattatgt
    22381 tggaaagttc ctgaatcctg catttttggt tttattaggt gctttgcttc tccttgcttt
    22441 tttgaatcca ctaggttctg taaatcaagc gcctattcag ccaaattatc aagagcatgc
    22501 ttttttcaca gggtttactc aaggatataa cacattagat gcgcttgctg ctttagcatt
    22561 tgggatcatc attgtcacaa caatccaaaa catgggtgta acaaaacctg ctgaaatcgc
    22621 taaagacacg atcaaatctg gtgcgatcag cattatattg atgggaatca tttatacttt
    22681 gctcgcttat ttaggtgcta tgagcttagg tagctttgct ctaagtgaaa atggcgggat
    22741 cacgctggct cagattgctg atcattattt aggtacttat ggcagtatct tattagcatt
    22801 tatcgttata ttagcttgct tgaagaccgg tattggtttg attacagctt ttgcagaaac
    22861 attcacagat ttgtttccgc aaagaaatta cgcattcttt gttgcacttg ctagtatcct
    22921 tccttgttta gcggcaaacg taggcttgac gaatatcatt caattttcgt tgcctgtttt
    22981 gatgttcatc tatccattag caatgacact gattttacta gtgttagttg gtcctttgtt
    23041 taagcagcgt acggctgttt atcgaatgac tacttatttt acattgattg cttcgatttt
    23101 tgatgggttg aacgcttgcc cagaaactat caaacaaaca cctattgttc aaaatctttt
    23161 atatgttgcg gaaagctact tgccgttttt caaattgggg atggggtgga tcgtaccagc
    23221 agttatcggt tttgttatcg gactgatttg gagtttcgct aagaaagaag aagtagcaga
    23281 ttgataaaag aggttgtgaa agagacgttc agccttgagc aataaaaagt aattttgaaa
    23341 aatggcttct cacatttttg cataaattta acttaatacc gagaaactga cttttgaaca
    23401 ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa gtattaagaa atattttgtg
    23461 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23521 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23581 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23641 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23701 aac
//
LOCUS       DAJSNV010000038        23459 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSNV010000038 DAJSNV010000000
VERSION     DAJSNV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.039"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1838)
                     /locus_tag="ON516_001409"
     CDS             complement(<1..1838)
                     /locus_tag="ON516_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344901.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMDQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVA"
     gene            complement(1907..2209)
                     /locus_tag="ON516_001410"
     CDS             complement(1907..2209)
                     /locus_tag="ON516_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344902.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGIILAAASIVVSLFGMFGQTPQGL"
     gene            complement(2227..2469)
                     /locus_tag="ON516_001411"
     CDS             complement(2227..2469)
                     /locus_tag="ON516_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3344903.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(2466..2921)
                     /locus_tag="ON516_001412"
     CDS             complement(2466..2921)
                     /locus_tag="ON516_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344904.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            3109..3300
                     /locus_tag="ON516_001413"
     CDS             3109..3300
                     /locus_tag="ON516_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344905.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            3444..5501
                     /locus_tag="ON516_001414"
     CDS             3444..5501
                     /locus_tag="ON516_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3344906.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(5537..7627)
                     /locus_tag="ON516_001415"
     CDS             complement(5537..7627)
                     /locus_tag="ON516_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3344907.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YNDGSAQVIDKSTGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(7624..7884)
                     /locus_tag="ON516_001416"
     CDS             complement(7624..7884)
                     /locus_tag="ON516_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344908.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(7897..8658)
                     /locus_tag="ON516_001417"
     CDS             complement(7897..8658)
                     /locus_tag="ON516_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344909.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKNLTTDQIESQLKDRVKKLSGDQLKLVSNGETKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(8674..9426)
                     /locus_tag="ON516_001418"
     CDS             complement(8674..9426)
                     /locus_tag="ON516_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3344910.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(9439..11415)
                     /locus_tag="ON516_001419"
     CDS             complement(9439..11415)
                     /locus_tag="ON516_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3344911.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGDEHSFQVGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSMFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNESAEIANPLTNSVDFVYNNNPFNQEEHHEKSKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            complement(11467..12138)
                     /locus_tag="ON516_001420"
     CDS             complement(11467..12138)
                     /locus_tag="ON516_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3344912.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(12220..13386)
                     /locus_tag="ON516_001421"
     CDS             complement(12220..13386)
                     /locus_tag="ON516_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3344913.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(13392..14081)
                     /locus_tag="ON516_001422"
     CDS             complement(13392..14081)
                     /locus_tag="ON516_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3344914.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(14208..14447)
                     /locus_tag="ON516_001423"
     CDS             complement(14208..14447)
                     /locus_tag="ON516_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344915.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(14514..14753)
                     /locus_tag="ON516_001424"
     CDS             complement(14514..14753)
                     /locus_tag="ON516_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344916.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(14867..15208)
                     /locus_tag="ON516_001425"
     CDS             complement(14867..15208)
                     /locus_tag="ON516_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3344917.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(15195..15524)
                     /locus_tag="ON516_001426"
     CDS             complement(15195..15524)
                     /locus_tag="ON516_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344918.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(16115..16447)
                     /locus_tag="ON516_001427"
     CDS             complement(16115..16447)
                     /locus_tag="ON516_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3344919.1"
                     /translation="MSYTGILSFEDICHYGKRCTATEKITKKLSIGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(17283..18323)
                     /locus_tag="ON516_001428"
     CDS             complement(17283..18323)
                     /locus_tag="ON516_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3344920.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKRKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(18672..18998)
                     /locus_tag="ON516_001429"
     CDS             complement(18672..18998)
                     /locus_tag="ON516_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344921.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(18985..19788)
                     /locus_tag="ON516_001430"
     CDS             complement(18985..19788)
                     /locus_tag="ON516_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3344922.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(20142..20432)
                     /locus_tag="ON516_001431"
     CDS             complement(20142..20432)
                     /locus_tag="ON516_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3344923.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(20568..20747)
                     /locus_tag="ON516_001432"
     CDS             complement(20568..20747)
                     /locus_tag="ON516_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344924.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(20744..21094)
                     /locus_tag="ON516_001433"
     CDS             complement(20744..21094)
                     /locus_tag="ON516_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344925.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANHNLAKEQMTTE
                     EIAEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(21087..22412)
                     /locus_tag="ON516_001434"
     CDS             complement(21087..22412)
                     /locus_tag="ON516_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3344926.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERSLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKEKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLGLDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PVTDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTAIVHASSMLEGARSITRSTLVGGHAGGNGGLKND"
     gene            22862..>23459
                     /locus_tag="ON516_001435"
     CDS             22862..>23459
                     /locus_tag="ON516_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3344927.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDS"
BASE COUNT         6927 a         4631 c         3612 g         8289 t
ORIGIN      
        1 gctacagaat cagcctgtga agattgattg atatgattaa aagcaaatac tgttcctcca
       61 cctagaagca agcttaaact tgcaatacat cccaccatcc atccgttaaa ggaacgctgg
      121 ggtgaaggag ttaaatttgc ttggttgttc actggcgttt gaacagcagc tactggttga
      181 ggatgtgtca gataatttaa ctgtgcttgt cgcaatgtat ccatttgttc ttgacgttcc
      241 ttacgagcag ctgcttcttt ttccttttct tgttctagcc gttgtttttc tatcgccatt
      301 ttttgttccg caagatcctg ttcacgtttt tttagttcta attgttcgcg ctctttttct
      361 tttttccgtt gtttttcttc ttgttctttc aattcttgtt ggtgaatttc atactctttt
      421 ttccattttg gaatttgtgc ttgtaattct tgttggatct gctcgtgttt ttccatcaga
      481 acttcagtcg tagtttgata gagtttattc agttgttcat ttaattcttt gctaatcctt
      541 agcttatcag tacgcaaccc tcggtaaaat tcttcttttc gttcttttat acgttctttt
      601 aataagtttt ctttttcttt cgataacgct tcttgattcg tctgagtaaa attgttgatc
      661 ttttctacac acaaggaata agcattgtcg cgtttctttt cgtaccgctc tttgattttt
      721 tgtacttctt cttcaatcac tcgctgttct tctttattta gttcctcttt tactttctcc
      781 caattagacg actgttccgt cgcatactca ttaatttctt gatctgtttc ttgttgacgt
      841 tcttcaaagg tggctaataa atcttcttgt gcaagctcat gccaatttgt tgattggaca
      901 gactctaact gttctaaaag tgtgccttta ccctgtgaga ttaattcttc tttactctct
      961 aaaaatgctt gataggtttg gggtaattga tatttcgaat aaagctcatt taaaaataat
     1021 ttgttttttt ctttcatcca ttcatgctct ggcggatatt gatccaaaat ataatcaaat
     1081 acagttgcct tttgtatctc tgtttcgttt ttttctactt cttcactgat aagtggttca
     1141 atatattcct gagaatcccc ttcagtccat gttgactctt cattagcttc aactggatca
     1201 acttctactt cggccgtctc ttcatcctcc attgtttctt caaagggatc tacctccaat
     1261 tgatccatca catcgttctc ttcctcaaat atcgatttct gagtcggaat atcagcttgt
     1321 tcgtcaacat agactgtctc tttttctttt gactccttct ttggaaatat tggtcgttcc
     1381 catggtactt cttcatttac ttcgacttca ctcacttgat tcacttttct ttggtcgctc
     1441 tctaactctt cttctaaaac ttgtaaaagt ttttcgtttc gataagactc ttccacactc
     1501 atagtttcat ccgtttcttt aaacacatca aggcggatta aatccatcaa tgtaggataa
     1561 aaaccttctg aaatttgata atccagtggg ccaaaaacgt attctgtgcc agccttttct
     1621 atttttcctt ctttatcatg taacgtgaga gtcaatttaa cagaagattg attctcttgt
     1681 tctaacactt gtcctaacac atgtagctgt tcttccgttt caatgacaga atatttaatt
     1741 gttttcggta aaaaggaagt acgctttccc actttcttcg ttgcttttct tattaactct
     1801 ttatccaatt ttgcatttcc tgaaatcgta aaaaacatga atattctcct ttcataaaaa
     1861 aaggagtaaa aagtagctat gtacttttta ctccttcatt ttgtttttat aatccctgtg
     1921 gggtttgtcc aaacattcca aataacgaaa ccacaataga agcggcagct aaaataattc
     1981 ctacccccat caatacatat cccacatgca tcttcccgtt ttcagcacct cttcgtccaa
     2041 ataagaagaa aagcccccca ataacaatgg aagcaacggc cgcaacgccg gcaaaaatca
     2101 ttaaattgtt ttttaagcta gttgctgcag tttgtaatgc tctaaaaata tcaaccgcag
     2161 ctactacttt cggttgtgtt aaaaaattca ttgtaattga ttcaaacata aaattgttcg
     2221 ctccttttag tgtttaataa tttcatcgaa aacaagcttt tcacgttcac gttgatagat
     2281 atatccttct aagtccttgt cttttccccg taataggtca aattgtgctt tttcaaggac
     2341 ttcttgtttc cactgtgttt cagatacacc gcgacgttct aagattttag acgcggcttt
     2401 ttcaaatggc gttttagctt ttgccactgt gattcctcct tcaggataaa taaaaagact
     2461 agcaatcact cgctagtctt ttgtaacttg tatccagatt tagtactgta ttcttttaat
     2521 atttcacttt tatctcgatg ttctttttcg tttgttggaa taaatttaaa cgtttctaaa
     2581 ccgtctttgt tatagggaac tattaaggct gttttaccta tgaataaatc tactccacct
     2641 gaatcgatta ccttatatct ttctaatcct ttatagtctt ttagcttctc aactgttatt
     2701 tgtacttgtt caggatgcca aacactttcg gaatattccc atgttgtatc atctataatt
     2761 tttaaattat gaacaagtcc gcctacttct tctttccaca ctgttcctgg ttttaacacg
     2821 ccattatctt gactatcttg cttatttttt tcactaccac atccacctaa aataacaatc
     2881 atggcaatca acgaagtaaa taccattatt aactttttca ttctattcct tctttcgttg
     2941 ttttatttta gtatatcaaa taacgaaagt agcaacaaac ctcattttga tacacgggga
     3001 acggatactc ataatttccc acctttctat agaaactttt cctcttgata ttgtgattta
     3061 ttacaaaaaa gagctataat aaagaaaaaa tcaagaggga gcattgcaat gtacgctgat
     3121 aaagagaaaa tcgaatggtt actatttgaa actggcaaga cagcatatga aattagcaaa
     3181 cattcttcag tcgcagaatc aacaattgcg gatttaataa agaaaaaaag tgcgattgaa
     3241 aaaatgcgat ttgataatgc tgccaaacta accaaatacg ccgaatccat ttcttcatag
     3301 catataaaca gccatcctta cactcttcat agggcggctt ttttatttca aaatttccac
     3361 ggtataccgt tgactacctc ggtataccgt ggtatattat gaatgtaaac aaaatattta
     3421 atcatttaat aggagttgaa aacatgaaat tattaaaccc aaaaactgtt atagctattg
     3481 gaacatttgc tgccacagtt gctcttgctg gatctgctga cgctgcttca actaacgtca
     3541 tgtatcgcct ttataatcca aataaccatg aacattttta cacctctagc acaaaagaac
     3601 gtgatcacct agtgaaaatt aagtggggta attatgaagg ccccgcttgg gaagcaccga
     3661 ctagcggtgg ccaccttgtc tatcgtttat acaataaagg ccttcgtgat caccattaca
     3721 cagctagttg ggatgaagtg aaatggttga ccaaaaatta cggttggact tatgaagggc
     3781 ctgcatggcg ttctgccgaa aaaaataata aaccgatcta ccgccttttc cttccaggtg
     3841 ttacctctgg ttcgcatcac tacactgcaa gttggaaaga agtacaatgg ttaaccaaag
     3901 actatggctg gaaatacgaa ggtattggtt ggtatggtgc tgacacctct aaacccaaag
     3961 tgaataaatc agctttagaa gctctttata cgagcgttaa gggaactaaa aaaggtgact
     4021 ataccgacaa tacctggaac gcattccaaa ccgctttaaa caatgctaaa aaagtgttaa
     4081 atgactcaaa agcgactcaa aaacaagtgg atagtgccaa aaacacatta gattctgctt
     4141 acaagggatt aaagaaaaaa ccagctccta ctgtgaacaa aacggaatta caagccttgt
     4201 acaaccaagt aaaatcaact gctaaaggag attttacaga aggatcttgg aataattttc
     4261 agactgcttt atccaatgct aaaaaagtac tagacgattc taaagcttct caaacccaag
     4321 taaataatgc taaaaatagt cttgattctg catttaaagg gttacaacac aaacctaaac
     4381 cttcagaata tgcggtaact gtccgtcata tgaatcgaca aactggaacg ctgctaaaag
     4441 aagaacatgt ctcagtaaaa gctggtacaa gttacactgc aaaagctaga accgacttga
     4501 ctaactttga agcaccacag tatcaagtaa atgggaatga aacacaaaca caaacgatta
     4561 acacaaatac cacatttact ttttactatg atgaagtctt tctagttgaa gtgaaagctc
     4621 gtccaaataa agtctcggat actgatccaa atatccaaga actctataat tacagtaaga
     4681 gttatcccgt cgtttatgga caaagcatca caattgaagc acctatgttc actaattttg
     4741 ttcttgatcc tagagaaagc tctagcaaca ctgtgacttt aaacaacgtt acaagtaatc
     4801 aaggaatcaa ttttgcctat actcatcaat acaacgtcac tgtgaaccat ataaacgttg
     4861 acacaaacgc tgttttatct actgaaacac aaacggttta cgagggagat agtttctcta
     4921 cttcttggaa aaatatgacc gatcaaaact acttcttgtg tagaaatgac gattcatctg
     4981 ttaccgttga taaaaatgga caacgtgtaa tcaataatgt tgaccaaaat cgaacgatca
     5041 ctttcaaata caaaaatatc tcactaagtg atttaaacaa ctatgtgcgt caaaaagaac
     5101 ttgcttggtt aaatagctac cgtcaacaaa atggcgttgc cccaatgcaa ttcaatgata
     5161 tcgttcaaca agctgcagat attcgtgcaa aagaattaca agtaagcttc agtcattacc
     5221 gacctggcgg cggtactttc caagatctct tagaaagctt aggctgctac ggtgctaaag
     5281 gagaaaatat caatagtttc tacctttacg ttgacgagat cctttcagat acaggagcag
     5341 taaatgctat ggttatgtgg aaagatagcg ctgctcataa cgcaaacttg ctttacaaca
     5401 atcaaagtat cgttggttta tcccatgctt atgaagtttc tgcagacgga tcactttctt
     5461 cagacaacgt atttattagt gccaatccca ttttcaaata gaaaaacaag gctgattttc
     5521 agccttgttt ttttgtttaa ttgaacttca gaactgcgta gccaaaagtt gctaacacaa
     5581 gtaatagtaa gctgaccgct aaaatcgtta ctaaatgtgt agaacctgtt ttaggaacaa
     5641 taacttgtgg caagttagga tcaaccccaa ttgtcacaac atttgtttta ttagaaacat
     5701 tatctactgc tactacggct tggttatcaa tatttttttc tgattgtaat ggagttttag
     5761 ctgtaaatat cagctgaatt tctcgtccat ctaaaagatt gttatttaag aagtcagcgg
     5821 aagcttccca cttcagcgtt cttgaattgc tatcataggt tagtgtgcct tcagaagtaa
     5881 tatcttttcc tgtactctta tctattactt gcgcagaacc gtcattatat gtcaaacgag
     5941 cttcaattgg atcttgaata ctaaattgtt tgtagtgatc tgacgcagcg tctacgcctt
     6001 ttgtgtgcca tttttgtgtg actagatatt gtacttcaga tcctgcgttt aaaggagctg
     6061 tcggcgtacc cttcttacca tcatttcgtc cgtttacatc ttttccattt tgttgtactt
     6121 ctttcactgg aattttaggg aaataaacgg taatgatatt tgtatccttt tctactttat
     6181 taatgatcga cgttccctga tttttggcta cactattttg attggccaat tcattgactt
     6241 ttacattgat cactaattcg taagtttcgc cgttatactt cattcgattt tttaatgtat
     6301 cagctttcgc tgcatatttt acctggtgtt ttgttttgtc ataggtcact tcaccagata
     6361 catcgaattt tttatttgta ccactttcaa cgtgtgcatc gacgtaatct acttcttttg
     6421 gtaaattatc gattaactca aatcgatcgt aaaccgctag taaatccact cctaatcgat
     6481 tcaccttctg tttgattcga tattgaatcg tgtctcctac ttgcaacatt ttatcgttca
     6541 cgttatttcc atttttatca acccctgttt tttcaggttg atcaggaaca acactaaaga
     6601 ttgtcgcaga agataaagta aaccaggttt gaacattaga agaaccaatc acaaacgtcg
     6661 gatttgcttc agatatttta aaggatacag taccttgacc aaacttcgga tctcctaatt
     6721 tatcatgcaa attattgaaa tcattatccg cttgtgtcag cttagagctt cctcctgggt
     6781 ggccacctac cacgttcaga ttgttgtaga aagatttaaa ttctgaaaga accgcatctt
     6841 gtctgacata ccaatctgtc gtattcattt tgtcatagtg ggcatattct cctttaagat
     6901 ccgtgctttt tcctcctgca gggttcaaag agttaaaact gataaatgta tctcctgaaa
     6961 attgaacggg gctaccgtcc tcatatgtgg ctactagctg cacattcatt tgtgttgaat
     7021 ggtaaacaat tcctgagaaa agattgtctg agatccaaag tactgttctt tttttagatt
     7081 tactcattgc accattcgta tttttatagc catcgtctcg aaaatgagtt tttgtatagt
     7141 tgttatctag tagattccac ggcaacgtat ccgagtataa gtgaatatta gatagaacta
     7201 gcttgatatt gacttttttc ccgttatacg taccgatttt tccatagtca atattgactt
     7261 tggtgttggt tgttacgcct tctaaaacaa ggtttgtttc accgtattcc ttttggaaag
     7321 aaggatagcg tatatttttg tttccctcca ctaatcctac gtagtcgtct gtaaaaccac
     7381 ctttgttggc atcaacatga tatttgtaaa ctttggacgc tccgtttaca gtaatatttg
     7441 tttttgcatt aggaacatct tttggaataa taaaattatt ctttacgttc tggattggat
     7501 ttgacggatc gacaataact ttttcagcaa agacattggc tgtttttaac ccaaacaatg
     7561 tacctaacat catcattatg ggaaaaacat attttaaccg tctggtccac gagtgtttaa
     7621 aattcatccc ttcatcccct cttttttata aatgactagt gaaattccga caataacgat
     7681 caaattgatc gtagtaaata atggattcct aatgagcttt tccataatat ctagcggcat
     7741 ggaagcagct ggtatgttta aaatcctcat taggtatagt tggtagcttg gcaaaataga
     7801 taattgagaa aataataaac cgcagtacaa aaagactgcg gttaaacaag aaagattgaa
     7861 taataataat ttgatcagtt tcatcttatt tcttccttaa tctctagaaa acaagtgagt
     7921 aacaaaatcc caacaattct caaagaagtt tttctttaca ccagtagaaa cgattttttt
     7981 tacttcttct ttaggttgcg aagcttctga tttaggataa atccacacat cctttaagaa
     8041 ttcaccgttt ttttcagaaa caggtgttaa tagcaaaata tcctcgctat tagaaatgtt
     8101 ttcaggactt gactcattga caatataata agcttgtttc tgatttgcct gtacttctat
     8161 cgagaattca aagacacctg tttgttgatc aattgtcttt gtttctccat tagaaactag
     8221 cttcaattga tcaccggata gttttttcac tcgatctttc aattgggatt caatttgatc
     8281 agtagtgaga ttcttaacat ccatcttttg taaaatgtcc gatacatcat aaacagaaaa
     8341 cttcgcgcca tttactcctt cttgtttttc aagcttttgc ccctggttgt ctaattgttc
     8401 ggaagaaata atcttttgaa cgtgaatggt ctgtttgact atttgatcgt cttcagcaat
     8461 tttcggttct tctgaagaat ccactgttga cgaatcgacg gtttcatcgc tgctttgaat
     8521 cgtgctgcta tctgacgttt ggattgtttg ttctgtgcta tccgtgcttt cttgactgtc
     8581 tgcaaacgtc aactgtgtgt aggcagtgaa agaaaaaaga gcggctgcac aaacaaatag
     8641 ccattttcta tttctcatgt tggttaccct cctttatttt ttctttttat aaacaaatag
     8701 cgaaccaatg atcatgactg aaagaaaaac agtacttcct aaaagccatt tccaagataa
     8761 tttttgtttc ttattttctt catcttttaa ttctgatttt gtaaaggtta ctcgcttccc
     8821 atacactagg aaacggtgtg tgttgatccc cttaggcgta catgtcatta gcgttactcg
     8881 atcttcattg ggatttggtt ctaagtaagt gacaaagttt ctttgtagtt cgtctttttg
     8941 cacttcctca atccgatcga tttgataagc gtgctgctca ccatcaactt tgatataaaa
     9001 cttttctccc tttttaactg aaggcaaatc atcgaaaaga ttatctttat aaagtccact
     9061 gtgccctgcg attaaaggat ttttaccgtt tcctcctgta atatcgccag ttccttccgt
     9121 aatccctaca ccattctcta aaattttgtc ggaagttccg tcataaattg gtagttttaa
     9181 agaaatactt ggaattgtaa gaataccaac aggatcgcct aactcaatct tagaaacatc
     9241 caaagaagaa tccgtttctg ctgccgttcc tgttcgatcg attgcggcaa tctgctgatt
     9301 ttgttggtat ttcgccacgc tcgcttcagc aggcatcaaa agtaagacac caacagctga
     9361 aagtaaccag aaagcaatga tccatttacc catgattcgc tttttcttag ggtgtctgat
     9421 aatcattagc tgatccttct aggctttctt cttaaagtaa acaactgcag aaattgcgcc
     9481 tgccccaagc aacaagcaag agatcaatac gtaagattgg aaaccgttgc taccagtcat
     9541 tggtacggaa gctttttgat tatttttgat cgtgattcgg ttttcttctt tgtatgaatc
     9601 tttgtctact ttgaaagaga tctcttttgt taatttttga tagttttcag gagcttttgt
     9661 ttcttccagt gagtaatctc cttcagttaa accagtgatt tcaaaatgac cctctttatc
     9721 tgattttagg attgctgcat ttgctacatc gtctacccat actgcttctt tgacaccatc
     9781 tttatctgta tcagtaagtc caccgtagta cttaccttct gcattcttag ctgcaaattc
     9841 tgcgccttca agaggagttg ccttaattcc agttccaaat agtccactat cgactttcaa
     9901 gaattttgca ccataagtaa caacatcagc ttttgatttt tcgtggtgtt cttcttgatt
     9961 gaaaggattg ttgttataaa cgaaatcaac gctatttgtt aatggatttg cgatttcagc
    10021 actctcattc acaaacatgt tataagtcat ttttagatct tgaccatgta aattcgcaac
    10081 tgtatcactt acttgtccat tatttacgat aaaatcgatc acaaaaccac tatcagaatt
    10141 ttcgatttta tagtcagtat tttcttttaa aacgactgaa ccatcattat ttgttacttt
    10201 aatatctgct ttttcagcaa cataagaaag tcctttacct gctgtatctc cccatttaaa
    10261 catgctgtat ggagaagttt cactcttcac accatttact gtaatcccat ctgttccacc
    10321 tgcaatatct gttggaatat gaatcgtccc ttgataatta acggcatcac caacttggaa
    10381 agaatgttcg tcccctgttc cgttggttac tgtcttatcg attacaggag ctttgtagtt
    10441 tacgtaggaa gcttttaaat cacttgtaac accgtcattg gtaatttcaa aggttaattt
    10501 attatgggca tcgttgcgag cgtctgcgcc taataaatat tgatcggctg ttggttcttt
    10561 atctgatccc actacgactt ctgaaggaac ttccacaaag tagtattttc ctaacgtcaa
    10621 atcagtcgca gtcagctttc cttgatcatc cgaaacaaga tcgcctagtt tgcttccttt
    10681 tccaggttct cctttataca attcgaattt agcgccttta agaggggttt gtttactttg
    10741 ctctgtacca tcatcaccga atttggttaa ttcaaaactc aacttagaaa caatattttt
    10801 aggatataag tgaatgtctt ttaagaaacc tgaacctgta ttgtcagtca ttggagcaat
    10861 aaccaccatt ggttttgctt tttgagtcac taatcctgct gcggacttac tctcgtaaat
    10921 aacatacaca tttccttttg agttcacgta cgctggttga tcagcaaaag taatctcacc
    10981 attttcatct acagctgaag taattttgtt ggttgcattc ttgacgtact cactgttaga
    11041 tcccttgtct tctacgtctt tcacgatttc gtccactttc gcgttggtta gatcagaatc
    11101 ttcagtcggt aatacttgat ccgtaatatt tcctaacgta aactcaacat caccataatc
    11161 tgccttgttg tatttcgtta cacctgtagg atattgatcg tgcgtataac catcgttttt
    11221 gatcccgtca atatcaacat tcaattgcgt tccttgatcg tacattaatt tatgaaccgt
    11281 gatattgacc ttctctggtg gcgttttttg cgtgacatct tctgcatgtg cttgataccc
    11341 ctgaaagcag cttgcaatga ttcctaacat tccaacaagc attccagctt tttttacata
    11401 tcgatttatt gccattttta ctctcctcat ttcatatttc aacgactcgc gttgattaaa
    11461 accttgttat tgtagctctc cctgtaccac gtatctttct tttccttgtg acgtatagac
    11521 acaagtaaac aacgttaata agggtttctc tcctttcttt ggcaaagaca agaaagagtt
    11581 ctcttcagga acgccatcga ttaatctaaa gtacccaggt acgataaatt tacgcgttac
    11641 cttgtaacga tagacgccat cgttactaac taagtccatc acctccccta atgacaactt
    11701 ttgaatatca gaaaataaaa cacctggcat ttccatgtta tgacctgcta ggacatagtt
    11761 tcctttgccc atttcagcat cttcataaaa ataagtagcc gctccaagac tcaaagtgta
    11821 ttgatttgcc cctttaaaaa tcggcaatgc taaaccaagt gcaggaatct gaatttgtgc
    11881 aaatacttgt ttattgacgc ccatttcgat tgcttctttt tgtatggaca acatttgccc
    11941 caaagacata tcattattga cgttcgttgc atcctcctta taactctttg catgtgcatt
    12001 gatttgatct acgggcatct ttttttctat attttgctta aacacctctc cttcatgttt
    12061 ttggtagcta acatagccaa acacactgca aacgacgatt aaagcgactc ctaaaaaacc
    12121 aaataaccat tttttcatcc atctgcctcc tttcagacaa aacaaaaaac actttttcac
    12181 aacgaaaaag tgttttctct tattactatt ctatttcatt tatctggtgg ttttatattg
    12241 gtgtgtagcc attgctaaca ttaagaatac aattggcacc caactgattg tcgttaatgt
    12301 ttcttccatg aacgattgac acatgatcgc taaaaaagcc cacaagaatg ttagttgtcc
    12361 atttgtcagc tcacgttttc ttacgagcaa ccataagaaa ctaagcaaaa gaataatcga
    12421 tagaacaaaa ccaatcaatc catagtgatt gacgagtgcg tgatacgtat tatgattacc
    12481 tagaatctga tttcctctat ggaaaaagaa aggtttcatc cctacaaata tttgtcctgt
    12541 tttttcgcct aatgtttcat agaatttgcg ccaaatttct tctcgtcctg taaataagtg
    12601 aactttgtct gacattgcag caaaataaga aatcggtaaa gctagaaccg aaactgctgc
    12661 atacatccaa aaggacaagt gtgtatttcg aaaaaccttc ttaggtaaaa tttctaagat
    12721 aatgaagata acaagcgcat ataatgctgc tttcgattgg caaacccaaa tcgttgcccc
    12781 agcaagaatc aacgctggta atgatagcaa tcgcatgtac cattttccaa atgaactagc
    12841 aaatcctgtg ataagaaggg ctaatgtcat taaagaacta cctatcgtat ttgtattgat
    12901 ccaaatatct gataatttgt tcgatggatt ccaaatactt tgtccttctg gtactggtat
    12961 ctcagtaaat actcgataaa ccgttacgag taaaaagaca tagaatacta caagatacaa
    13021 gctacgatca attacagtaa atttcgtcca ttttacaatc aaaataatag caataattgt
    13081 cggaacgatc agatcaccat agaaagtatc tcctcggtag cttctaaaaa aagaaaagaa
    13141 aaggtaaaaa agaatgcctg ctgcaatgaa gaatagatcg tttctcttta ccttgtacag
    13201 attcaaaaga aacaaacagc ctacgaaaaa cgtcactaac tgtgcagcat tatcataaat
    13261 aaataatatc gtaggaggat ttaaccccac cgtccatatt aaatatagaa tggataccaa
    13321 cagtaacaga aaaatggcac tcattttatt gatcaatttt tctacaagtt cttgaatgtg
    13381 actcacttcc cttatttatt gattttttct acattgacta attcacactg gatcacatcg
    13441 cactttttca tattatttgc ttgaaaggct aacagtgtga gtagtggttt tttacctgct
    13501 tttggcaagg ataaaaagga attgttttcc ttagattgaa ctacctggct gtaatcagaa
    13561 atttttcctc ttttggagac ttcatactga tacttgtaat gagcatcgat tagatcaatt
    13621 tcttctcctt cagataaatc agtcatatcg gataataaaa cgcttggtgt atctacgtta
    13681 taaccagcaa gaatataatt tccatttccc atttcagtat ctagataata agtagaagct
    13741 actcctaaac taagcgtgtc gctattcgct ccttgaaaaa cagggagaat aaatcctgta
    13801 gaagaaaaaa caagttttcc agcaatttca cctttgagat ccttttctaa tgcctgtttt
    13861 tgattagtaa tcagttgtct cttagaaata tccgttaaac tggttaaatt atcttgatag
    13921 gttttagcct gttcctgaat atggattgtt tttgttacac catctacttt ttcagcgatt
    13981 ttgtcctttt gataaccttg ataagcaaaa taactaaaaa gaagaaaaac aatgattacg
    14041 cccacgtaag gattaaataa agttttattg tttttattca tctatctacc ttctatatat
    14101 gaattaattt acctacatct aaccaagata aaccatgtta aattttacta ttctttttat
    14161 tcaataacaa tgccacatca taattgatca ttttcatatc ataatcgtca agaaacttct
    14221 aataatagtt ggcaattgga acctgttttt acataagttc gcctcaactg ttcaaagttt
    14281 ttcattgaac ctaacaaaca agctttctct tttcctgtta tcttcatatc attgatatat
    14341 tgcatgacag ataatctttt aaggtgtttt tgtttcatca tttttagcaa cttttcttcc
    14401 atttctttcg acactttttt tctgttttcg atttcttcat aattcatgag ttttcctacc
    14461 tttcttgttt tttgcaaaaa taaaaaagcc tatgatcaca tacgctctac acttcagtct
    14521 tgggttatct ttttctttcg tttaattact ggccgtgctg gtttcaaatc cttgattcca
    14581 ttatgggttc tccaataatc gaattctttt cgtaatcttt ctttttccat atctgttcct
    14641 ttagacaggg atttgaatac atctttaggt gaataaccac gagctaacag ctgtacatac
    14701 cttccttttg tcaaagtatc catatccatc gcctctaaaa ctggtcgcgc catctaattt
    14761 tcctcctaaa taaattcctt actcaatgcc taaggtaata ctaccttttt attaatcgtc
    14821 gtgactcctt ctaagccttt atctgtaaaa tttattcctc cagtttttat tcttctttat
    14881 ccgctacaaa ttcaatgatt tcaacgctga caatataatc actcaaatat gaccaagtat
    14941 cttcaacaga taattcgtct ccattatcat tcgtaagcgt cattttaaac cattcattac
    15001 cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat gtttgatcgt
    15061 ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg cctcttctaa
    15121 acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatcgta tgtgttcccc
    15181 atcggctatt cggatcataa tttttcattt gttgtaacct cctcataatc agttagtact
    15241 ataaaggtat ctttgttttt atccaaaaaa tctaataaat cttttgtaga agatatctct
    15301 tcatcaactt caccataata aataccttcg ataattgttg ctatatctga aaaactaatt
    15361 tttactggac atttatcgtc taccgcattc aataccgaca aaattgcttc tttttctgca
    15421 atctctcgtt tgaatttctt tggagctaat cgcatgacac tcaagtcatt ttcaagcttt
    15481 tcaatccatg cagttttact cattttcgca aatttttgaa tcattcctct accacctttg
    15541 ctatctacta aattagtttt acttcataac gaatatcttt atcgtttttc gttcactcaa
    15601 aaatatataa aataaatcga ctcgtttatg gcgtccgaaa cgagaaaccc tagtagacag
    15661 cggtaaatgc tcgattgatc caataatcaa cgtcctcgca agtatctaca tgtactgcag
    15721 cacacgacac aatttcttgt gcgtcttccc cattacgaga agacctactc cttattccat
    15781 gagtttgcgt caaagcctag atcccttagt agctgctcat gattaatcta ggcgctattc
    15841 taacttgtct aacttctaaa ttccctcttg tttactctgt gcatattatt tacacaaatt
    15901 catttcacca ctatccaaac aagggcattt actcgattga ccaatttttt acgttaccaa
    15961 tcgtaaaaac ctaccccgtc actccccgaa gggaattgtt gatagatcaa gtctactcct
    16021 tatttttcta gtgattctaa gttcctttag aagtctcgac aatattttca agcaagcgaa
    16081 aaacgataaa atacaagtta tgagaatgat caattcagct gattaaacgc cccatccctt
    16141 ttgcggtaca acgcaagact ccattttttc ttcgaccaat cagtacaccg ttttggtcat
    16201 aaacatgttt gattgttgga tatagctctt ttaaagggat aggatctttt aaaattattt
    16261 gcttgcgttt cccagtgtaa taaatcattt cttctgaaac tttgtctttc tggattatat
    16321 atttcttaca ctgcacaaca gctttgcttt gcccaattga tagtttcttc gtgattttct
    16381 ctgtagcggt acaccgttta ccgtaatgac aaatatcttc gaaactcaaa attcctgtat
    16441 atgacataaa gtaatcctct cactttcttt aaatattttt aataagtttt tcttgttatt
    16501 tttcaaaaat agagaattga ttttcgttca tgacaaacaa aaaagaggga acgcatgttt
    16561 gaatgcgttc cctctaattt cagcgttcgt catccggcaa agagccacac ggacattttt
    16621 ataacattat gcttaggcat agattcatta caatatcacg tatttttaac cttaatagag
    16681 ccaaaaaaag tggtatcact tttgaatatc tgttatactg agtccgttgc gggtaatacc
    16741 gtaacactga agggcacgca ttaaggcgtc atcttaatgc tttcctgtag gacactgacg
    16801 gagattacca gtctcccgag ccttacaggg gcctttcttt tttttgtatt ttcaattatc
    16861 acaatttatc gtccaaaagt gcagaacgaa aatcaattca tcacaagcat atcagacatt
    16921 taaatttaaa acaactaaaa atatacaaaa aagtgtaatt gaatattagc agcactgcct
    16981 gaaaagaagt aatatcaggc ttttcagctt ggggaaataa gtgaattacc tctcttcatg
    17041 aattattata actcaaaaaa gcacatatgc aaacactagt tccctatttt tttgaatgaa
    17101 aaataactaa aatatctaac gattatgact ttaaaaccta cgtttgtgat tgttttcata
    17161 aacttttaat ctaaaaactc cggaataata actatttatt tgtaacattt gtaataaaat
    17221 taaaatcata aaaaagttct aatttaaaat tgatcatttt tatagtgatc gctttttatc
    17281 aactactcta gccaattttc cgtactaact gttggtaatt cgttaaatgt ttctctttgt
    17341 tcgtttgcaa gctttgccgc aatagctttt tcgacaaacc aattttttac ataaacgaaa
    17401 attagattct gcaaattgtt tactttttca gttttttgtt tttgataagc tttcagtaaa
    17461 gtcatatata atcctttgtc agcatcaatt ccataagatt caagttcttc ataaacaaca
    17521 atagtttgct ctaattcttc agcttttctc tttgcattat gaatagtttg gacggtttgt
    17581 tgagcttcac tgtaacttga agaaaatgtt tttatcaagt tcaatacatt ttctggtaca
    17641 aatgtattta tactgtcctc tttcatgatg tcgacgaagt tatctagtaa aacttgattt
    17701 tgtaactgat cagtttcagt gtctattatg tgtctattag tgtctataga attagaatag
    17761 aatagattgt ctgcgatttt cgcaattacg gttgagtcct tgatatgact ggattttgcc
    17821 gtatttgcga ttttcgcaat tacggtagta tcattgattt ctcgcggttt tgccgtattt
    17881 gcgattttcg caattacggt ggtatcagta ttgtcttcct cttccatgat tttaaagacg
    17941 tcttgattcg ttacttctgg cttgcctaag tacaataaat taggcataga ttcaatctta
    18001 ccatcacgtt tatttactct tcctctcttc tgtttcagaa gacctgcttt ttctaattcc
    18061 tttttgattt tagatacttt accttcgcgg cattgtaaca atttcattaa ttcttcaact
    18121 gtgaatatga agaatatatt atcatcagaa tctacccagt tatttttgac tgaataatca
    18181 aatcgatctt taagtagcat ataagcaatt tttgcatcat ttgatagatc catgtatttt
    18241 tcgttagtaa aaaatacctt aggcatttga taatattttt cacgataaat ttcttttgat
    18301 ttgtaataat taaaatcatt catagccaat cctctttctg taggatagcg ttttgttcaa
    18361 aactatgtta aaatgaatta aacaaatata tctatccact atatattttt ctactgctga
    18421 taagtcgcca aactttgtta aagcagtagt ctttttttat ctttttttag tttaccttaa
    18481 aaaataaatt aaagcaaacc gctctatttc aaggtttaag ctcatagtaa ctatatcacg
    18541 atattacaat aatattatat cacgatatcg taatattaca atatgaagat aagcgactta
    18601 tcagctttga tactactgat ttttataaaa aaaatatcac gatattatga tatcgtgata
    18661 ttgaatgtaa attaaatttt attttgtttc tttgctagtt tatatccttc aatcaagtat
    18721 tcttcaggta tggactttaa tagcgcatct tctataacgt ctggtttact tttgtctgat
    18781 tcagcaatag tcgtccaata tttgagtgct gacattagaa ttggatttat acgaatgcta
    18841 tctgctgttc ttacactact tccttgatct tttatgactt tacttatatt tttcttggtt
    18901 aattcgattt cttctacttc ttttggtttg tagttctttt tatttttatt tcctagtagt
    18961 ctacttactg cagcttgact gcgattatct tctgccatca aaatcaccaa cttcttttaa
    19021 atattcttct gtaaatgctt tatagtcatc ttcagctgtt gagggtttat taaaattgat
    19081 tagatctgat ccagcagctt gcgcttcgtc aacggttatt gtcctacgaa tataagtttt
    19141 gaaaattttt gcaccaatga ctctagaaat ttcggttgct gcagacgtca tttctttaga
    19201 aatgttataa cgcttattat acttagtaaa tagaactcct cgtacttcaa gcttgtcgtt
    19261 cgtatattct tttaccgttt caatagtttc acttaacttt attagtcctt ttaatgagaa
    19321 atacgcagct tcaactggaa tgataatttc atcagaaaca gtaaatgcgt tgactgtaag
    19381 cattcctaaa cttggtggac aatcaattat aatataatca tagttgtcct tgattggttg
    19441 aagtactctt ttaagcctgt gttcacttcc tacttgagta aattccctct ctgcagaaga
    19501 aagggctatg tcggctggca aaacatcaat tcctttatag tccagaatag catctgtaat
    19561 gtcagcctcc ccttttaata cttcataaat tgtaggttgg tttgttccat ctatatcgaa
    19621 acctacatta tctgtagcat ttccctgagg atcaatgtcc actaaaagta catttttacc
    19681 gtaaacaaat ttcaaacatg ctgctaaatt aacgctagtc gtagttttag aaactccacc
    19741 tttttgattg gctactgaca atattcttgc cattaaaata tcccccattc atcttatatt
    19801 ataatataat tatatcataa tattgtgata ttgcaatatc gtatatgcat tatattgtaa
    19861 tattgtgtga ttattttgtt ttaaagatat atatcggttc ggatataaag attattcata
    19921 tgttattcga aattaaaata atcacttttt attactttaa aattttttat taatgataag
    19981 aaatatgttt tgcgagcagc aactagaata atttaaacat tactaaactg tctatattgt
    20041 tactaataat tcccttctta tagtgcattt tcttagaaaa aaaacaatat tacgatatca
    20101 tgatattaag atatcgtaat attatgcaat tgtgattgat attatatatg tttttttatt
    20161 tttttatcat gtttaatctt gaaggaatca tattcttttt ctaaagacag gagcttttca
    20221 taaacatcag acatgtttaa actagaggcc aggcttaaat cataaacgaa tgaagcaggt
    20281 aatgatttta ttctacgttg tcgggttatc cagcttgcaa ccgttccttg tgggtaggca
    20341 tataactcac aaaattgttc tacagtgatt cctaaattct tgataatgta aacattgatc
    20401 gggtgcgagt aaacaaatgt attgatagac atggtgcgta agcctacttt cttttattat
    20461 tgcgtttgca ataatagttt aacacacgca ttattctagt atctactcct atttgtaaaa
    20521 acaaaaaagg attcatagag ataactctat agaatccgta aaatacctta tatatataac
    20581 gggaagatat aaaacctaaa attatgataa cctgctgcag cttctacata gaaagagaat
    20641 cgctcaacac caacctcatt tatcaaacgt tggccataac cccaaatctc ttctgatagc
    20701 tgatcaaagg ttatatactt tagctcatat tgctggataa tgttcattct aatcgactcc
    20761 atttattatc ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt ttgtctttgc
    20821 caacgtagat ccccagctca tcactaccta atattttgcc aatgatatcg tctggatagt
    20881 atccttcagc atttcgttct tctttttgaa tacatactaa tctatttttt atgactgcag
    20941 cgttcagtac ttccgcaatc tcttcagtcg tcatttgttc ttttgccaaa ttatgatttg
    21001 cacgttgctc attgtccgca tcgatctctt ccgtgtgatc ggacaaatag aatcctgccc
    21061 atttcaaccg tccacgatcg ttataatcaa tcatttttaa gccccccatt gcctcctgca
    21121 tggccaccaa ccaatgtact tctagtaata ctacgagcgc cttctagcat agaagaagca
    21181 tgcacaatcg ctgtaaaacc atatttttgt ctgatcttat caataatctt atccaggttt
    21241 tcttcatcga tttgtttttg cggatcacta aataaatcca gctgaagcga atcggtgtac
    21301 acaagcttgc tataggttat tccgacgtgc cttacttctt gtccttcata atacttatca
    21361 aataagaaaa gacaataatt ctgcaattcc tttgtattgt ctgtgacagg tattttcatt
    21421 tggtgcgaga atcctggtct cgtttctaag atcgacgtgc caatgtgtaa atggacacat
    21481 cctgttttac aattatgctg tctgatacga atggctactt gttccgccat ttcttttact
    21541 actagttcga tttcatttct cctagtatag tctcgtggca aaacttgtga attaccatag
    21601 gatttctctt tggttggttc tggtgggatc gctatatctg ttcgatcgat cccgttagca
    21661 tggaagtaca gctgtaagcc aatcacacct aatctattct taatcgtata aggattccag
    21721 ttagcaaggt ctctaatgct caaaatcccc atttggttga gccttttttt cattcgcgaa
    21781 ccaatgcccc aaaagtccgt catttcagga atattccata ctttttcagg aacgttttcg
    21841 tacgtccatt ccgcaacaaa tccttcatta tgctttgctt cattgtctaa gcctagttta
    21901 gccaaaaggg gattatcgcc gacacctaca gtagcgatca atcctaattc ttctttaata
    21961 cgttcttgga tcatttgagc gagcttcttc cttcgctgac ttcgtgttcc ttcagtcgta
    22021 aaaagattca gtgatcgggt cacttttaag attgattcat cgatcgagta aatcagtaga
    22081 tcttcatcag ccacatatct tctgaaaata ttatttacct gcatatttcg cttgatatat
    22141 aggttcatac gtggtggaac aacgtgtagt gttttaggaa atggttgtgg taagtcacgt
    22201 ggtctactca cattagaaat cccatatttt tcctttgcag ccggtgaaga agctaaaatc
    22261 aatcctgatc ctgtattgtc cgctcgactc atcacgacta attcagtcgt taatggatct
    22321 aaactccttt ctatacattc gacacttgcg taaaagcttt tcatgtcgat taaaaagtaa
    22381 tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt tcacattata
    22441 caaacatatg ttcgatattg taaagaagaa atagctaaat acataaaaaa agaagcatag
    22501 gtatgctatc accctgcttc ttcttttgat ttgtatgatc aatttattct atcactttac
    22561 taattaattt gaaaatctaa taaaaactct cactctaaaa gagtgagaga ctgcaaatta
    22621 gagtacggtc ttttttggag ttccgttctt taatattaac aaatgaataa aatccttaca
    22681 acatttgaga ataagttatc agtagacact tacaaaaaat tctcatacta tttcctccat
    22741 cattagtttt tgtctatata aggtttaccc taaatagaca ctggtataac attgaaaaat
    22801 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
    22861 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccaagttga
    22921 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgctacaat
    22981 tactcatcga cctgttttcc aagaagtatt ggattacgtc cgagatcaag atatttttat
    23041 tgtggaagct attgatcgac ttggaagaaa ttacgacgag atcatcgctt cagtcaatta
    23101 cttgaagaag aaaaatgtgc agctgatcat tacaagtcta ccgatcatgg ccgaagcaat
    23161 tggcaatcct ctcctggata aattcattaa agatttgatc attcaaattt tggccatgat
    23221 cgctgaacaa gaacgtaccg aatctaaacg acgtcaggca caaggcatta agattgcgaa
    23281 agcgaacggt gtttataaag gacgtcctaa gctatacagt gccaatgcaa aagatcccca
    23341 acgtcgtctt gtttataaaa acatcgttga ggatctaaaa aaaggtgttg cgatcgctaa
    23401 gattgcaaaa gactacaatg ttacgagaca aaccgtatat agaataaaaa aagacagca
//
LOCUS       DAJSNV010000039        23408 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSNV010000039 DAJSNV010000000
VERSION     DAJSNV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.040"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..1690
                     /locus_tag="ON516_001436"
     CDS             29..1690
                     /locus_tag="ON516_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3344928.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1760..2107)
                     /locus_tag="ON516_001437"
     CDS             complement(1760..2107)
                     /locus_tag="ON516_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR3344929.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2191..3132
                     /locus_tag="ON516_001438"
     CDS             2191..3132
                     /locus_tag="ON516_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344930.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3133..4176
                     /locus_tag="ON516_001439"
     CDS             3133..4176
                     /locus_tag="ON516_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3344931.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4182..5252
                     /locus_tag="ON516_001440"
     CDS             4182..5252
                     /locus_tag="ON516_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344932.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5242..6195
                     /locus_tag="ON516_001441"
     CDS             5242..6195
                     /locus_tag="ON516_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3344933.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6207..7217
                     /locus_tag="ON516_001442"
     CDS             6207..7217
                     /locus_tag="ON516_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3344934.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7502..8323)
                     /locus_tag="ON516_001443"
     CDS             complement(7502..8323)
                     /locus_tag="ON516_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3344935.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8420..8698)
                     /locus_tag="ON516_001444"
     CDS             complement(8420..8698)
                     /locus_tag="ON516_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344936.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8966..10975
                     /gene="metG"
                     /locus_tag="ON516_001445"
     CDS             8966..10975
                     /gene="metG"
                     /locus_tag="ON516_001445"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3344937.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11454..12041)
                     /locus_tag="ON516_001446"
     CDS             complement(11454..12041)
                     /locus_tag="ON516_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344938.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12216..13538
                     /locus_tag="ON516_001447"
     CDS             12216..13538
                     /locus_tag="ON516_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3344939.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13693..14469
                     /locus_tag="ON516_001448"
     CDS             13693..14469
                     /locus_tag="ON516_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3344940.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14462..15031
                     /gene="rnmV"
                     /locus_tag="ON516_001449"
     CDS             14462..15031
                     /gene="rnmV"
                     /locus_tag="ON516_001449"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3344941.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15024..15908
                     /gene="rsmA"
                     /locus_tag="ON516_001450"
     CDS             15024..15908
                     /gene="rsmA"
                     /locus_tag="ON516_001450"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3344942.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16359..16988
                     /locus_tag="ON516_001451"
     CDS             16359..16988
                     /locus_tag="ON516_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344943.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17112..18221)
                     /gene="ugpC"
                     /locus_tag="ON516_001452"
     CDS             complement(17112..18221)
                     /gene="ugpC"
                     /locus_tag="ON516_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3344944.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKYGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18430..18852
                     /gene="mgsA"
                     /locus_tag="ON516_001453"
     CDS             18430..18852
                     /gene="mgsA"
                     /locus_tag="ON516_001453"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3344945.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19175..19711
                     /locus_tag="ON516_001454"
     CDS             19175..19711
                     /locus_tag="ON516_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3344946.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19751..21514)
                     /locus_tag="ON516_001455"
     CDS             complement(19751..21514)
                     /locus_tag="ON516_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344947.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21517..23232)
                     /locus_tag="ON516_001456"
     CDS             complement(21517..23232)
                     /locus_tag="ON516_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3344948.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7540 a         4288 c         4771 g         6809 t
ORIGIN      
        1 tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc
       61 ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc
      121 agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat
      181 gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt
      241 ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga
      301 gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa
      361 atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt
      421 agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga
      481 cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga
      541 attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc
      601 atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag
      661 tgataaagct gtttataatg gaccattcac attagaagat tttgatggac caggaacaga
      721 tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc
      781 tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg
      841 ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc
      901 ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa
      961 ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc
     1021 tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga
     1081 tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc
     1141 ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga
     1201 ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata
     1261 tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc
     1321 attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg
     1381 ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata
     1441 caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac
     1501 agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc
     1561 tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa
     1621 aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt
     1681 tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca
     1741 acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag
     1801 caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga
     1861 gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct
     1921 gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg
     1981 gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga
     2041 ggttttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt
     2101 ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg
     2161 tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa
     2221 cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg
     2281 caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag
     2341 atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt
     2401 tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca
     2461 catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt
     2521 ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca
     2581 gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct
     2641 gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat
     2701 ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct
     2761 gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca
     2821 aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg
     2881 attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg
     2941 gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca
     3001 aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt
     3061 attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg
     3121 agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg
     3181 gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct
     3241 tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt
     3301 tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct
     3361 catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc
     3421 aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct
     3481 caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat
     3541 gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca
     3601 ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt
     3661 gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc
     3721 atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc
     3781 atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat
     3841 caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa
     3901 catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct
     3961 gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct
     4021 tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta
     4081 tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga
     4141 ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag
     4201 tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg
     4261 tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa
     4321 aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg
     4381 gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa
     4441 tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga
     4501 tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag
     4561 agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac
     4621 gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga
     4681 ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca gcgcttgatg
     4741 tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat
     4801 caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg
     4861 tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc
     4921 aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc
     4981 gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat
     5041 tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg
     5101 aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga
     5161 caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt
     5221 acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag
     5281 tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt
     5341 tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc
     5401 cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat
     5461 ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc
     5521 tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa
     5581 ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat
     5641 ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca
     5701 ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc
     5761 atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg
     5821 ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg
     5881 atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg
     5941 ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct
     6001 gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct
     6061 gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat
     6121 gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt
     6181 ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga
     6241 cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc
     6301 atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca
     6361 cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag
     6421 caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct
     6481 tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc
     6541 gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga
     6601 taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata
     6661 atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag
     6721 ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag
     6781 aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat
     6841 tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt
     6901 atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag
     6961 attatcttga acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat
     7021 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
     7081 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
     7141 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
     7201 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
     7261 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
     7321 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
     7381 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
     7441 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
     7501 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
     7561 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
     7621 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
     7681 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
     7741 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
     7801 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
     7861 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
     7921 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
     7981 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     8041 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     8101 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     8161 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     8221 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gcttcatgtt
     8281 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     8341 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     8401 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     8461 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     8521 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     8581 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     8641 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     8701 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     8761 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     8821 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     8881 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     8941 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     9001 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     9061 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     9121 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     9181 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     9241 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     9301 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     9361 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     9421 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     9481 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     9541 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     9601 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     9661 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     9721 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     9781 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     9841 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     9901 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
     9961 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
    10021 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
    10081 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
    10141 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
    10201 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
    10261 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
    10321 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
    10381 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
    10441 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
    10501 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
    10561 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
    10621 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
    10681 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
    10741 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
    10801 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
    10861 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
    10921 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
    10981 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
    11041 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
    11101 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
    11161 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
    11221 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
    11281 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
    11341 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
    11401 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
    11461 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
    11521 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
    11581 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
    11641 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
    11701 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
    11761 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
    11821 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
    11881 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
    11941 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
    12001 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
    12061 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
    12121 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
    12181 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
    12241 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
    12301 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
    12361 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
    12421 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
    12481 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
    12541 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
    12601 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
    12661 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
    12721 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
    12781 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
    12841 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
    12901 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
    12961 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
    13021 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
    13081 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
    13141 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
    13201 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
    13261 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
    13321 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
    13381 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
    13441 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
    13501 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
    13561 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
    13621 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
    13681 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
    13741 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
    13801 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
    13861 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
    13921 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
    13981 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
    14041 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
    14101 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
    14161 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
    14221 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
    14281 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
    14341 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
    14401 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
    14461 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
    14521 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
    14581 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
    14641 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
    14701 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
    14761 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
    14821 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
    14881 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
    14941 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
    15001 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
    15061 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
    15121 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
    15181 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
    15241 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
    15301 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
    15361 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
    15421 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
    15481 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
    15541 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
    15601 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
    15661 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
    15721 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
    15781 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
    15841 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
    15901 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
    15961 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
    16021 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
    16081 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
    16141 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
    16201 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
    16261 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
    16321 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
    16381 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
    16441 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
    16501 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
    16561 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
    16621 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
    16681 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
    16741 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
    16801 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
    16861 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
    16921 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
    16981 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    17041 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    17101 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    17161 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    17221 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    17281 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    17341 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    17401 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    17461 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    17521 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    17581 ataaatccgt atttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    17641 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    17701 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    17761 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    17821 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    17881 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    17941 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    18001 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    18061 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    18121 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    18181 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
    18241 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
    18301 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
    18361 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
    18421 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
    18481 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
    18541 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
    18601 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
    18661 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
    18721 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
    18781 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
    18841 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
    18901 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
    18961 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
    19021 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
    19081 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
    19141 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
    19201 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
    19261 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
    19321 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
    19381 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
    19441 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
    19501 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
    19561 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
    19621 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
    19681 tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga
    19741 ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa taaccatctt
    19801 tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc atcactaata
    19861 tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg gtttttcctt
    19921 ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg actgagctgg
    19981 tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca atcgtcaaca
    20041 gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta tctaattttt
    20101 ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca ttgatttctg
    20161 cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg tcaaatagcc
    20221 aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg atagagcgga
    20281 tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat
    20341 taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt tgacctggat
    20401 aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa aaagaaacat
    20461 gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca tctggcaaat
    20521 caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg
    20581 tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac tgacttgagt
    20641 agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc aatgccaata
    20701 ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga gaagacgggt
    20761 ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca gcatttagca
    20821 gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt ttctggttac
    20881 cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt ctatcgaaat
    20941 ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt gtagaacaaa
    21001 gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa agaatgatga
    21061 ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa atattgtcca
    21121 tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa taacgaagtg
    21181 gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat aagtaagcta
    21241 accgattgct taacacttgg atgagccatt gactgactac gttgattaaa acgataccac
    21301 cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct ccaatcattt
    21361 gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca ttgatgattc
    21421 ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga atgaatcggc
    21481 ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct gtgatgcgac
    21541 gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct gagcagcaat
    21601 cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga cacgttggga
    21661 gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt
    21721 ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct tggcaatcaa
    21781 agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc cattttccat
    21841 gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct gagcgatttt
    21901 cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa ggttttcacg
    21961 aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct gacggagatg
    22021 atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg aactgacatc
    22081 atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg gaccaatgat
    22141 tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa gaccagaatc
    22201 aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag aaaaatcggg
    22261 agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct ctttcacccg
    22321 atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc
    22381 caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt gcaagctacc
    22441 aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat tcatgatcaa
    22501 ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat tgttcgctag
    22561 atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa atgcgcggat
    22621 gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta gtttttcttg
    22681 cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga ttgagaaaat
    22741 aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag ccatgaccac
    22801 tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg ctaaagctag
    22861 ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt
    22921 caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta actctgtacc
    22981 aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa tcagaccaat
    23041 cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt cattcatttt
    23101 tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga agacagcttc
    23161 aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg cataacgtaa
    23221 taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa aacaagtact
    23281 accacattgt agcttaggaa atttagaatg aaaagagaac aagccttttc ataaatgaaa
    23341 gttttattat gaagaagctt tagaaattga atagactatt ttttttaaaa aaaagaaaat
    23401 gagaaaac
//
LOCUS       DAJSNV010000040        23034 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSNV010000040 DAJSNV010000000
VERSION     DAJSNV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.041"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            61..2502
                     /locus_tag="ON516_001457"
     CDS             61..2502
                     /locus_tag="ON516_001457"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3344949.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2832..3536)
                     /locus_tag="ON516_001458"
     CDS             complement(2832..3536)
                     /locus_tag="ON516_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344950.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3723..3968)
                     /locus_tag="ON516_001459"
     CDS             complement(3723..3968)
                     /locus_tag="ON516_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344951.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3985..4836)
                     /locus_tag="ON516_001460"
     CDS             complement(3985..4836)
                     /locus_tag="ON516_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3344952.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5002..6006
                     /locus_tag="ON516_001461"
     CDS             5002..6006
                     /locus_tag="ON516_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3344953.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6247..6603)
                     /locus_tag="ON516_001462"
     CDS             complement(6247..6603)
                     /locus_tag="ON516_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3344954.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6971..8158
                     /gene="tuf"
                     /locus_tag="ON516_001463"
     CDS             6971..8158
                     /gene="tuf"
                     /locus_tag="ON516_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3344955.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8346..10937)
                     /gene="mprF"
                     /locus_tag="ON516_001464"
     CDS             complement(8346..10937)
                     /gene="mprF"
                     /locus_tag="ON516_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3344956.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10963..11724)
                     /locus_tag="ON516_001465"
     CDS             complement(10963..11724)
                     /locus_tag="ON516_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3344957.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12002..12523
                     /locus_tag="ON516_001466"
     CDS             12002..12523
                     /locus_tag="ON516_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR3344958.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12582..13442
                     /locus_tag="ON516_001467"
     CDS             12582..13442
                     /locus_tag="ON516_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3344959.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASMIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13627..15942
                     /locus_tag="ON516_001468"
     CDS             13627..15942
                     /locus_tag="ON516_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3344960.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16124..17965
                     /locus_tag="ON516_001469"
     CDS             16124..17965
                     /locus_tag="ON516_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3344961.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            17989..19098
                     /gene="rpoD"
                     /locus_tag="ON516_001470"
     CDS             17989..19098
                     /gene="rpoD"
                     /locus_tag="ON516_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3344962.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19318..21417
                     /locus_tag="ON516_001471"
     CDS             19318..21417
                     /locus_tag="ON516_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3344963.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21613..22476
                     /locus_tag="ON516_001472"
     CDS             21613..22476
                     /locus_tag="ON516_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3344964.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22581..>23034
                     /locus_tag="ON516_001473"
     CDS             22581..>23034
                     /locus_tag="ON516_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3344965.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7750 a         4034 c         4805 g         6445 t
ORIGIN      
        1 gaacaagagc tatttgtttt tacagcaaag aatggtatat ttttgtagga ggtggcttac
       61 ttgaaagaag tcgttatgat agatgctgca agaacgccga taggaaagta tcgcggaagt
      121 ttaagtccat ttactgcagt tgaattaggc acacttgtga ctaaaggatt attagataaa
      181 acaaaactga aaaaagataa aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt
      241 aatggtcaaa atgttgccag acagattgct ttgaacagcg gtctccctgt agatgtccca
      301 gcaatgacga tcaatgaagt ttgcggatct ggtatgaaag cagtgatctt agcacgccag
      361 ctgatccaat taggtgaagc agaacttgtg atagccggcg gaacagagag tatgtctcaa
      421 gcacctatgc tgaaacagta tcagtcagaa acaaatgaat atggtgaacc aatttccagt
      481 atggtcaacg acggattgac tgacgcattt tcaaatgcac atatgggatt aaccgcagag
      541 aatgttgcaa cacaattttc tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc
      601 cagttgaaag cagcacatgc tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc
      661 aagatttctg atgaagacgt gttatctgag gatgaagcag ttcgtggaaa tagtacattg
      721 gaaaaactgg gcacgttacg tacagtattc tcagaagaag gaactgtaac agcaggaaat
      781 gcttccccgt tgaatgacgg tgcctctgtg gtgatccttg catccaaaga atacgcagaa
      841 aataataatc tgccttattt agcaaccatc aaagaagtag cggaggtcgg tattgaccca
      901 agtatcatgg gaatcgctcc aatcaaagcc attcaaaaat tgacagatag atcaggaatg
      961 aatttatcaa caatcgatct ttttgagatc aatgaagcat ttgcagcttc ttctatcgta
     1021 gttagccaag aactgcagtt agatgaagaa aaggtcaata tatatggagg agcaatcgca
     1081 ttaggccatc caattggtgc aagcggtgca cgtatcttga ctacattagc ttacggattg
     1141 cttcgtgaac agaagagata cggtattgct tcattatgta ttggtggagg tttaggcttg
     1201 gcagtcttgt tagaagcgaa tatggagcaa acacataagg atgtgcaaaa aaaaaagttt
     1261 tatcagctta cgccatctga acggcgcagt caattaatag aaaaaaatgt attgacacaa
     1321 gaaactgcgt tgatttttca ggaacaaaca ctttctgaag agctctccga tcacatgatc
     1381 gaaaaccaag tgagtgaagt ggaaatccca atggggatcg cgcagaattt tcaaatcaat
     1441 ggcaagaaaa aatggattcc gatggcaaca gaggaaccat ctgtcattgc agctgcaagt
     1501 aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac ctcagcggct gatgcgaggg
     1561 caaatcgttc tatcaggaaa atcggagtat caagcagtga tcaatgcagt taatcatcga
     1621 aaagaagagc tgattctttg tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc
     1681 ggtgttcaag atatctctac tcgggaattc atggggagtt tccatgcata cttatctatt
     1741 gattttttag tggatgtaaa agatgcaatg ggcgctaaca tgatcaactc gattcttgag
     1801 agtgtggcaa acaagttacg ggagtggttc cctgaagaag agattctatt cagtatttta
     1861 agtaattttg ctacagaatc attggcttct gcctgttgtg aaatcccatt cgaacgctta
     1921 gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat
     1981 gcgaagcttg acccttaccg tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa
     2041 gctgtagtgg cagctacagg aaatgataca cgtgcggtta gcgcttcgat ccacgcctac
     2101 gcggcaagaa atggattata ccaaggatta acagactggc agatcaaagg ggataaacta
     2161 gttggaaaac taacggtgcc tttggctgtt gccacagtag gcggagcttc caatatcctg
     2221 ccaaaagcaa aagcatcttt agctatgtta gatattgatt cagccaagga actagcacaa
     2281 gtaattgctg cagtagggtt agctcaaaac ctggctgctt tgcgtgcatt agttacagaa
     2341 ggaatccaaa aaggccacat gggacttcaa gctcgttcat tagcgatctc aattggggct
     2401 attggtgagg aaatcgagca agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag
     2461 cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga
     2521 agaataaagc aagatttaca gttattttat ttactgcaga tcttgctttt ttctctatca
     2581 gaaaatatgt ttattattag aattttttat agttaaggtt aggcatctta agaaatcagg
     2641 caactccaag atatcttcag gtagaagaga agtcgcctaa cattgtagga tcataggtga
     2701 tttgttggac aggtcaagct gatatttgag cctgatgtta gaaggaattt ctttttactt
     2761 gctgtagaaa ttattatatg actaaaaagg ctgggatttt aaatcccagc cttttatgac
     2821 aaatagaatt tttatcgatc agtaatcgct gtcgctgcaa attgttcgat caaaggaatc
     2881 aaagtcaatt tgactttgta tttccgttcg agtgcttttt cgataacgaa ctcattttca
     2941 gtagtcatag aaatagttgc taatttacga ggttcatcag catttttttc attaataact
     3001 acttcatgtt cattttgttc tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa
     3061 tcagcaaaac ttaaagcggc attcgtatca gttgagcgtg tattttcagt tgtgtccgct
     3121 tttggttttt ccgttttttc ttctggcaca ctgactgttt cttctgtaac tggtgtcgca
     3181 acagcttgcg cagttggttc atcgtttttc aatcgttgat cattccagta agacacgatt
     3241 tcaataagaa gattgtcacg gaatcgttcg aaaatcccgt tttctcctga acgaattctc
     3301 tttaatacag tattgaagtt gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt
     3361 ttcccagcta atccgatcgt taagatttga cggtttttgg gatcaagcat gacttgtaac
     3421 ggcaaactat aacgtcgcaa gctctcttct tctagagaac ggaataattg tcctcttaca
     3481 ccatccaatg aaggacggaa ttgatttaat aatttttgtt tctgtttttt cttcatatgt
     3541 tctctccttc gctcataccc tttcgattat aagaaaaaaa cagccaaatg aaaaggagta
     3601 atgcaaaaaa acttgcgtat acgcaaatta caacagaaaa atttgcgtat tttcccgttt
     3661 tttgaaagaa aaataaaaaa tggaacggat tatgtatata tacataatcc gttccattct
     3721 ttctattttt tcggtatcaa ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt
     3781 tcgtgccagg gtttgaccat tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt
     3841 tgatcatgtg tccagtttct tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag
     3901 acaaaatcgg aatgaaaagt ctgggtaact tcttccgaga gagaattcaa gcgttcgatt
     3961 ggatccataa aatcaccttt cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc
     4021 gactacacca tctaactgat cgatttgttt catttcttct tctgttaatt caaaatcaaa
     4081 taattcagta ttttctttga ttctgcttgg tgtcactgac ttaggaagag gaaggaattc
     4141 gtgttgcagc gaccaacgca aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat
     4201 ttgtttcatt tcaggcacat cgaagatttt accagtgcct aatggactat atgcttcgag
     4261 gatcatatca tgtttttttg catactcaac tacagttgtc tgtaattcgc ctggtgcaag
     4321 gaaaacttga ttgaccatcg gcataatttt tgctgttttt gctagtgtat ctagatggtg
     4381 gggtaagaaa ttgctcacac cgattgcttt gattttacct gcttcataca gttcttcaaa
     4441 tgctcgccat gtatctgcat ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg
     4501 ccagtggatc aaaaacagat ctaaataatc tgtttggagc ttcttcaatg attcttcaaa
     4561 agaagacatc acaagttcat agctgtggtt tgcattccat aattttgttg tcaagaatat
     4621 ctcttctcta ggaattccac tttccttaat cgcttggccg acgctttctt cattcttgta
     4681 tccttgggca gtatcgatat ggcgataacc tgcagccagc gcttctttaa cagaggaaac
     4741 agcgacatcc ccatcaggtg tttgccatgt gccgaatcct actaccggga tttcgtaacc
     4801 atttgataga cgataagtgc ttgttaatga attcatactt tcagctcctt ttttattcgt
     4861 tcaactataa gtgtagcgca tttttcgaaa aaaagaatcc attttgtttc tactcttgaa
     4921 atttttgttt aaaagaaaat gattttgatt ctggtatttt tgattttttt tccgtaaact
     4981 aagacagaag ggaatgagaa aatggatatc tatttaaaaa aagcaattct gcatattatt
     5041 gacagagaaa ctggttctcc agtcttttca caaaaagagc tggatctgac aaaagaatat
     5101 attcgtgatt ttcttcaaaa gaagatccaa aaaatctctt ctgctcaaac aaaaactgga
     5161 caactagcag aagaaagcat gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg
     5221 gaagcaagtg aagcaatcgt tcagcggtgg ttcgatatct accaagaaag tgaagaagca
     5281 cctagtgcag atgtatttgt tgtcttgtat gaattagata cagtgatgta cttatcttta
     5341 ttgaaagtca attatcgtga ggcttacact cactttgttg aagcagacga agcagggatc
     5401 gacaaccgct tgattttgaa tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa
     5461 gcattcgctg tgaatttgac tgattggacc tacgaattaa ttgaaaaaaa atatgagttt
     5521 tctggcaaaa aagagcttta tttttctgga agagtgatcg aaagtgttcc tgcaccttct
     5581 ttagaagaaa atgtaaaagt gatccaaaaa gtagccaagc atcttggaaa aaaatttgag
     5641 acagaagaat ttgatatcat ggctgatttg aaagaagctg tctatgatac gatagaagaa
     5701 aaaggtcggt tggatcatga gatgattgcg gaaaaagtct tcaaggaaaa catcacggca
     5761 aaaatggctt ttaaagaaga agtacaagag caaggatttg tcccagaagc acctcctgta
     5821 aaagaagtac aagaaatttc tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac
     5881 gggatcgaac tgatcgtacc agtagatgta tatcgcaacc cagatttgat tgaatttgtg
     5941 aataatccgg atggtacgat atccgtgatg atcaaaaatg tggatgaagt attgaatcgt
     6001 ttataaaaat gactagaata ttaggtgttc aagtaccagt gatcagttaa atgaattgtg
     6061 tcaagagaag gatcggaaaa gacgaatata acaaaaaaag agcgtgagac aaaagtaaag
     6121 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttct
     6181 tatttcttgg agttaaacgc ttctgtccca gcctcttttt atctaataaa attttaagac
     6241 catttactaa ttatcgattt tttctgccgc atttttaggc acttcagaag cctttatttc
     6301 ttcccagctt aatacacctt tacgaggatt gactttgagt ttaagatacg cattcatctt
     6361 taatggatgt tctctttctt cgcgaagcgt cacctgtttt ttttcaccat ctttattata
     6421 agccgcttgc ttgtaggtat aagtcgtgaa tttctctccg ttatctgctt gggtagctga
     6481 catttcgcca ttcgtcgtga tttttgtata ataagcttcg ccgccgtaaa aataatgata
     6541 agctgcaaaa cttccgccga gaaatatgcc taaaacgaca agtacgccaa ttaatttttt
     6601 catgtacttc acctctttcc ttattattaa cgaaatagaa gatttcgtct attgaaagtt
     6661 gttggcaaca gctgaatttt aaacttttcc tcattttgtt cttattgctt caaaagaaag
     6721 ttaaggacta tcttgaagtt tttctcaaaa aacacatttt tttatttata aaaggtactt
     6781 ttttcgcgta taagacagac gagtgctctt agttgacttt gaccaatatt gacgtataat
     6841 aagcttagtt cgaatcttac aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa
     6901 aaagactata ataaaaaggt gagagctgaa atttttcagt gatctatatt atttaggagg
     6961 tttgtaaatt atggcaaaag aacattatga cagaagtaaa ccacacgtta acattggtac
     7021 gattggacac gttgaccatg ggaaaactac tttaactgct gcgatcacta cagtattagg
     7081 caaaaaaggc ctagcaaatc ctcaagacta tgctagtatt gatgcagctc cagaagaacg
     7141 tgaacgtggg attacgatca ataccgcaca cgtagaatat gaaacagaaa aacgccacta
     7201 cgcgcatatt gatgcaccag gacacgcgga ctatgtgaaa aacatgatta caggggcagc
     7261 acaaatggat ggtgcaatct tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg
     7321 tgaacacatt ttattgtcac gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa
     7381 agttgattta gtcgatgatg aagaattgat cgatttggta gaaatggaag ttcgcgagtt
     7441 attgagcgaa tatggtttcc caggcgatga cactcctgtg atcaaaggtt ccgcattaaa
     7501 agcattgcaa ggcgatccag atgctgaagc tgctattatg gaattgatgg atacagtaga
     7561 tgaatatatc ccaacaccag aacgtgatac agataaacca ttactattgc cagtggaaga
     7621 cgtcttctca attacaggtc gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt
     7681 tcgtgtcggt gatgaggtag agatcgtagg gatcaaacct gaaacacaaa aagcagttgt
     7741 aacaggtgta gaaatgttcc gtaaaacgtt agattacggg gaagctgggg ataacgtagg
     7801 cgtgttgtta cgggggatcc aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc
     7861 aggttccatt actccacata caaaattcaa agcagaagtg tacgtgttga caaaagaaga
     7921 aggtggacgt catactccat tcttcaacaa ctatcgtcca cagttctact tccgcacaac
     7981 tgatgttaca ggaacaatca cattgccaga agatacagaa atggtcatgc ctggtgacaa
     8041 cgtgacgatt gatgttgact tgatccatcc aatcgctgtt gaaaacggaa cgacattctc
     8101 aattcgtgaa ggcggacgga cagtaggttc aggtatcgtt acagagatcg aagcataaga
     8161 aacgcgactc gaatattttg gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag
     8221 gatttctgtt tggaccgctc tttgttcttc aattgtataa gaagagattg tagaagcatg
     8281 aactttcgtt ttacttcagt ctcttttatt catcatgaga gtgttcatct atcggtatgc
     8341 agtttttaat actttcttcg tatccatttt ttcaaaccac ttattctacc ttgtgtctgc
     8401 tcaactggcg cattatcaat aatcagcaaa gcaatcataa catatgcaat ccaactatct
     8461 ctagaatata gagtataacg tgagacccag tttgtagcgt acttgctttt atattctctt
     8521 aatccatgga aagaataaaa atgagaacca aactcgtaaa ccaagtatgc aatcctttcc
     8581 tgtaaaaaac tctttctgga agttccgaca tttgaaagag gggccatccc taaattgaac
     8641 cattggatat gttcttcttt catataggca aacaaatgca agaacaagaa atccatactg
     8701 ccagaaggtg cttttttaga atcatatcgc atcagatcaa tcgttcctac tttattgttc
     8761 gtgtaagtag gcatgatcgt tgcaaaagcg acaatttctt cttgggtatt tttgacaact
     8821 gcaataggcg ctctttgtag ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc
     8881 tttcgtgaac ccaaccaatt atcagagata tttttgaaag tactcatttg ctcagtagaa
     8941 aatggtggtt gtaagacatc aaatgtaaat ccttctcgtt cgatccgatt caagacagca
     9001 cgagtgccct tcattttttt ccctgaagtt gtaaaactat tcaggtctac ataagcttct
     9061 tcccccatct taatgaaatc gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt
     9121 gtttcgtaga aaactggtaa atagcatagt cgatctgttt cttcgataaa agcttcgatt
     9181 gcttctggaa aatcttcttt cttaccagaa ggatcaccca ttacaataca tttattattg
     9241 aagcaagcga attggagaag aacagttggt tcaccgtctt tttcataggc aaacatccgc
     9301 ttatctttta agaagatcaa ttgactatcg gagtttcctc cataagttgt aaggatcttc
     9361 agggcctttt cttcattaaa ggcttcgcca atctgtttct tttctccttg aaggaaatga
     9421 acaaatagaa cgatcatgaa actaacagcg atgatggcca acaatcccga aaaccaaatc
     9481 ttttcagaag gaaataagaa aaaggaaata aaatggtgac gccgatgagg gaaatccggc
     9541 agattgtata ccccaataac gatgtaaagc aaggacagtg caccaataat gattccatcg
     9601 atcgtacgcc actcccatga atagatcaat tgttcccgaa ataattcgtt tttagaagca
     9661 agaatgatca gaagcagaat agacaagaaa accaccggtg tgaaactaaa atcacttaat
     9721 aaaacataaa gtaaagctag aacaatcaag aagattgtag gcaaatatgc tcgtttcaca
     9781 cgtgctgcga tccctctgcc cataatgatt aaaagaaatc ccaatagtat actaggaaat
     9841 tggataataa aatgaaattt caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa
     9901 gcttgcggga tagtggcaga aagtactagc atgattccag aaaagtataa taagacaacc
     9961 acgattttat gcgcaatttc tgttgctaat tgtttaggga taccagaata acgctgatca
    10021 aaagtggaac cgatattttt gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag
    10081 tagaacaggc gatagagcaa gatccacaca acgactattt ctcttggcac accaatagcg
    10141 gacaacccga taatcatcat tacatcgaaa ctcccaattt ctcctggaat catagacacg
    10201 attcctatca ctgaagcagc aatgaataat ggaattgttt gccacagatc aatccggatt
    10261 tccattaaat aaccaacaga aaggaaacta aacaggacac cggtccattc caacaatgaa
    10321 actagcaata gctgtccttg agttttaagt gaggtaccac caaggaatcc tttctttttg
    10381 aaagtagtaa ataaaaagac gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa
    10441 tattgcttca aaaaaggagc aactggcgtg aagactacaa gtaaaaaaga aagcagactg
    10501 taaatagaaa gtccagccat caaaaacaag aaaactttcg ataatgcttg gatcacgttt
    10561 ctgctctctt tcttttgtcc atatagttca gagcgaagcc ccacactaac aaacccgccg
    10621 aaaccggcaa tattgttgat cgtattgatc agccagcttg tctcaaacag gtaacgagga
    10681 ttctgctttt gttcaagcat ccgattcaaa ataatatcat aaccaaccat aggtaacaca
    10741 gagataagac caatcaatag cattagtccg cttttccata caggtatagt ggcaaacgtt
    10801 tctcctaatt gctggataga tagagtttta ccgatggaca tcaattcacc gacaataatg
    10861 acaatgacag aaatcaaaaa gattgttttg aacagtccta ggtggttttt cagccattgt
    10921 atggtatttt ttaacaaata aatcaccttc tattcctaat agttatgttt cagaaagaaa
    10981 ttgaagaact tctcgattga agcgttcagg atcttttcta gctaattcat gtccttgttc
    11041 tttcacaagc acaaaggaag cttttggtat agttttagct atatacaaag aatgttttag
    11101 cttgattaca tctttttttc ccacgatgat caaagtgggg gagttgattt tttttaaatc
    11161 attttctgta agcccgatgt catgcagcaa aagtttgata actaaaagat ttttgcgaag
    11221 acttgggcga aataaagaga gtaaccatac ccatgcataa tgaatattac tgatgactct
    11281 ggcagaaaaa cgaacgcctt taacaagcgt gtttccagaa tttaaaatca atcgatgaac
    11341 ttttttaggg aagctgctgg caaataccag tgctagattt gctccatcac taaatccaag
    11401 aaaatctgcc tgatcgattt tttccagcaa catgatcgta ttcagatcct cggccattaa
    11461 ctgaaaattc aacatagatg cttcgtttgt cgaacgacca tgtcctcggc tatctactag
    11521 atacacggta tagtagcgtt cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc
    11581 gtcattgcca tgcagtaaaa agagagggaa gccttgaccg ctcttttcat agtaaatttc
    11641 gctcccatct ggcatttttg catatttctt ttcttttttc acataatcac ctactgtgta
    11701 aagtataacg attatatcag tcattgggca attgttttta ctgaaaaaca acgatcattt
    11761 tcgtaagatg tcaaaaaata caggaaaatt cattttttct taaggcgtac acagaaatca
    11821 aggaagaaat agccaagaag ataaagaaat agtaagataa aaaaagatgt caaaataact
    11881 aggtgatgaa atgaagcgaa aacgattatc tattatccgt attctttttt acatgatcat
    11941 tctgacggtt attggtgcgg tgttgttttt atgaggaaga acttatcgtt cggtaaaacg
    12001 agtggaaagc tatcaaatag aaataacaac agctgcaaaa aattatcata tggaaaacta
    12061 cgagaatttg atcaaggcga taatattgac cgaatcaaaa ggaaaaggga cagatctcat
    12121 gcagagctcg gaaagtctgc atggagaagc tgagatcatt gataatccag ctgacagtat
    12181 tgatcaaggt gtcaaatatt tggcggaaat gatagtagaa gcaaagaaac aaggatgcga
    12241 tttatggaca gctgtccaag catataattt tgggaaagat tatattgcct atgtaaaaaa
    12301 tcatggcgga gaaaacacag tagaattagc agaagcgtat tctaaagatg tgctgagccc
    12361 acttttagga aatgaggaag ggaaacaata ccgatattgg cggatccagt ccttgcttta
    12421 caatggcggc tatctctatc ataatggagg taacatgttt tatcctgata ttgtcaaaat
    12481 gaatgaaaaa ataatagaga cctatgaaaa agtctttaag taaaatagca ggaaatgttc
    12541 aatcatgaat aaaattaaat gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa
    12601 tggaaaatca agaagtgggg tacacaacca aagactggat gttacggttt gtgaagggaa
    12661 tgtttatcgg ttcaggattt atcttgccag gagtcagcgg tggtgcatta gcagctgtct
    12721 ttggcatgta tgagcggctg atttcttttt tagcccacct tacaaaaaat ttcaaagaaa
    12781 atgttttgtt ttttctgcct gttggtctag gcggagtgac aggcatcttt ctactttctt
    12841 ttttagtcag ttttttatta ggtgcgtatg aaacaacgat attatggttt tttgtcggat
    12901 gtatcgctgg aactgttcct gcgttatgga aagaagcagg gaaaaaagga agagatactt
    12961 ctgatgtgat catcatggtt gttacttttg ttctggctta tttgttttta ttatacggcg
    13021 ctcaattatt tgacgggcaa gtcgatcaga attttggaac atggctgatt gccggcggat
    13081 tgattggttt gggtatgatc gtcccaggat tgagcccttc taattttttg gtgtatatgg
    13141 gattgtataa agcgatggct gacggattta aagaagtacg acttgaagtg attgtgccga
    13201 tcggaattgg tgggattctt tgtgttcttg gattgtcaaa aatcatggat tacattttct
    13261 caaaggcata tagcaagcta ttccatttca ttttaggtgt agtttttgct tcaatgatta
    13321 tgatcattcc taccgattac gcgggattgg gagttgctgg ggtactagct tgtcctgttc
    13381 tttttatagc cggtacagca ctaggttggt ggatgagccg tttggaggaa caatataagt
    13441 aaagatagat tgataataaa aacagcacac gggtgattca aacaaaaagg ttggcatctt
    13501 ttttgtaaaa tcattcgtgt gtttttctat aaattttata aaaacaagtt gtttttaatc
    13561 ttggaacacg ctagacttaa acacgtgatt atttttgaaa gttggcggaa aatggagaaa
    13621 aagaaaatga atggacttac acagcaagag gtagttcaga aaatggcttc tggccagcaa
    13681 aatgactacc aggaagatgc atcaaaaagt acaaaacaaa tattcagcga taatatattg
    13741 acgttattca attttctgaa ctttggtatt gggatttgtc tgttatttgt aggtgcgtat
    13801 tccaacatgg cgtatttagc aattattgtc gtcaatatcg ttattggaat ttatcaagaa
    13861 atacatgcaa gaaatcttgt acggaaatta tcgattgttt cccaatcaga agtactagtg
    13921 atccgggacg gtaaagaaca atcgatttct actaaagagc tggtattagg agatttggta
    13981 aaaatcggag caggagaaca gataccagcc gatttacagg ttatatctgg acgggcagaa
    14041 gcaaatgaag ctcttttgac aggcgaatct gatttgattg tcaaagaaac tggagctgaa
    14101 ttgctttctg gaagcttttt gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt
    14161 gcagataatt atgctgtccg cttgactagc gaggcaaaga ctcacaaagc tattcgttct
    14221 gaacttgtag attcgattcg caaagtatcc aaattcacta gtttcgtgat tattcctttg
    14281 gggatcatac tatttttgga agcactgttc atccgcgatg cttctatcaa aatttctgtt
    14341 attgcatctg cagcagcttt gttaggcatg cttccaaagg gtttggtatt gctaataagt
    14401 attgcattaa cgacaggggt aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg
    14461 tattcaattg aaacgctggc ccatgtcgat acattatgct tggataaaac agggaccatt
    14521 actgaaggga aaatgagtat ccagcgggta gaagcactac atccgctgta cgagaaagcc
    14581 atcccagaaa ttatggggac gtatctggca gaaagcagtg ataacaacat cactatgcgg
    14641 gctatgagag attattttga gctttctaat cggtatgatg tgacaaacaa agtcgcattt
    14701 tcttctgatc gcaaatgggg cgcgatggaa tttccagaac tgggagtcgt gtacgtagga
    14761 gcgcctgaac gtttggtgga agaagatcgg cttccggaca gtctaaaaac agcccaagcg
    14821 aatggctatc gcgtacttat gttggggatc gctccaaatc agcaattgaa cgaagaaaaa
    14881 ccagcatttg ttgagccggt tgctctgttt gaaatcgatg atccaatccg aaaaaacgct
    14941 caagaaacac tagcttatct tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac
    15001 aatcctttta ctgtctccaa tatcgctaga cgtgcaggat taccgaatta tgatgcgtat
    15061 gtcgatcttt ctcaaattac tgaagaaatg gaagtgcgag aagctgctca tcgctatacg
    15121 gtttttggac gagtgagtcc gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc
    15181 ggcagaacgg ttgcaatgac aggcgacggt gtgaatgatg ttttggcgtt aagagaagca
    15241 gattgcagca ttgctatggc agaaggtgat ggagcaacac gtcagattgc gaatctggta
    15301 ttgttggact ctgattttac tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc
    15361 aacaatgtga cgaaagtctc aggcatcttt tttatcaaaa cgatttattc atttatttta
    15421 tcgattatat gtgccgttac agcaattgga tttccgttta tccctatcca aatcacgctg
    15481 attgatttag cgattgaagg gtatccttcc ttctttttat cttttgaaca agataaaaga
    15541 aaaatcactt accgttattt gcctacagca cttattaatg ctttaccaaa tgccttgctt
    15601 gtagtagtga atatcattgc ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct
    15661 gaaactacta ctttgatgta ttacttgctg attgggatca gttgcatggc agttgtaaaa
    15721 gcttgttatc ccttcaaccc attgcgcatc ttcctagcag taacgacgat cgtagggata
    15781 tatgtagctg caatgctgtt ccatggaatt ttggaagtaa acttagggtt gggagagagc
    15841 gtaggatact tctttgtatt catgctcgta aatattgttc ttcgagtatt gtactggaag
    15901 gctgatattc aaaatcagtt actgaaaaga gtagcaaatt gatgcttaat gagatagaaa
    15961 tgtgttatac ttgaaaaagc aatgaaatat gatacaatca aacatgtata attgtgtcct
    16021 ttaaaatcgc actgatttca gttagtgcga ttttttaccg gttattctcc tgaaatatct
    16081 tctgttaaac ccaaaagata tcaatagagc agggggtgaa aaaatggctc aacggattcc
    16141 tcaagaggtt atcgaaacag tacgcagtca aacgaatatc gttgaggtga ttggacaata
    16201 tgtccagttg aaaaagtctg gtaaaaatta cttggggctg tgtccttttc atgaagaacg
    16261 tacaccgtct ttttctgttg cagaagacaa gcagattttt cattgctttg gttgtggtaa
    16321 aggcggaaac gtgtttactt ttcttcaaga actagaaggt ctaagttttc cagaagcagt
    16381 ggtaaaagtg gcagacttcg aacagatacc gttagaagat caatggcgtc agcgtactat
    16441 agctgtccaa aatcctgaat ctgctacggg gaaactgatt gctgctcatg aaaaagcagc
    16501 ggaaatctat catcacatgc tattgcatac aaaagctgga cagccagctt tagaatattt
    16561 gcaaagtcgt gggttgtctt tagaattaat tgaagaattc aacataggtt ttgcacctaa
    16621 tgagcgttct tttcttcagc aagtattcaa gaatgaatca atttcaaatg aattgttaga
    16681 acgctctgga ttgtttgtca cgcatgatga tggccgtatg tctgatcgtt tttatcaaag
    16741 agtgatgttt ccacttcgta attctcaagg ccagacgatc ggtttttcag gacgctggct
    16801 tgcacctgaa aaagggcagg aaaaagatca gcccaaatac ttgaatagtc cagaaacaga
    16861 actgttcaat aaaagacaag ttttatttaa tcttgataaa gcaagaagcg atatccggaa
    16921 aaacggagaa attttgttgt tcgaagggtt tatggatgtg attgctgcat ggcaggctgg
    16981 tatcaaaact ggattagctt ctatggggac aagtctaaca gctcaacaga tccaacaaat
    17041 ggaaaagctg acgaaagaat tggtcttttg ctatgacgga gacaacgcag gttttgaagc
    17101 aaccaatcga gggattgaat tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt
    17161 cgttccagaa aagcttgatc ctgatgaata tctccgtaag tatggagaag catctttcca
    17221 agaattagtg gctcatgggc gcgaaacagt ctttactttt aaatcacgtt atttaaaaca
    17281 agggaaaaat ttagataatg aaaaagacaa gatcgaatac ctcgaagaat tactagttga
    17341 acttagccga gttgtttcac caatcgaaca agatatgtat ctatctcaac tgtcaacaga
    17401 gtttcagatc ggcagagaga cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca
    17461 gcaaaatcgt caagaacctc aggaaagtca acacactcag gttacgagac aagaaactgc
    17521 acggaaaaag agacctttga cgcaagtaga aaaggcagag cagattctat tatatcgtgc
    17581 atttaaagaa gtttctgtcc gtaatatttt aaaagaacga gaatttcagt ttattcatga
    17641 tgcatatgca gaagtttatt ttttattcga tgcatatgtc tcacaacatt atgaattcaa
    17701 ccttgcagag tttttagact ttttgcaaga tgaaaattta aggcggttgg tggtggaaat
    17761 cgaatattta ccggtggcag aagaaagttc gccacgagaa atccaagact tgctagatgt
    17821 gatcagcaaa tctggcttag ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc
    17881 cagacgaact ggaaacaaac agcttgagct tgaattgacg attgaaatca tcaatttaac
    17941 gaaacagtta aaacaagcca aatagctact caaggggggc cttcttttat ggcaaatgga
    18001 acagataaaa aaacgtatga agcagcagta gcagctttca tcaaagaaaa caaacccaaa
    18061 cgacaagttg tatatgatga tttatccaat cagcttgcta caccattcac tttgaatgca
    18121 gatgaaatgg acaaattgat ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa
    18181 aatggtgatc catcaattca cagcttgaag agtgctgaga aaaaagctga acaagcaaaa
    18241 acagaagatc tttctgcgcc aaccggtgta aaaatcaatg acccggttcg tatgtatctg
    18301 aaagaaattg gccgtgtctc ccttttaact gcggaagaag aagtagcgtt agcattgaaa
    18361 atcgaagaag gcgaccaaga agcgaaacag cgtctggcag aagcaaacct tcgtttagta
    18421 gtaagtatcg ctaaacgtta tgtaggacga ggcatgcagt tccttgactt gatccaagaa
    18481 ggaaacatgg gactgatgaa ggcagttgaa aaatttgact accgcaaagg atttaagttt
    18541 tcaacctatg ctacatggtg gattcgtcaa gccatcactc gagcaatcgc tgaccaagca
    18601 cgtacgatcc gtattccagt tcatatggtt gaaacaatta ataaattgat acgtatccaa
    18661 cgtcaattgc tgcaagacct tggaagagaa cctacgccag aagaaatcgg tgcggaaatg
    18721 gatcttccaa cagaaaaagt ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt
    18781 gaaacgccaa tcggtgaaga agatgactct cacttaggtg atttcataga agaccaggaa
    18841 gcaacaagtc cagcagaaca cgcagcttat gagttactaa aagaacaatt ggaagatgta
    18901 ctagatacat tgactgatcg ggaagagaat gtccttcgct tacgttttgg tttagatgat
    18961 ggccggacga gaacattgga agaagtaggg aaagtatttg gtgttactcg ggaacgtatt
    19021 cgtcagatcg aggctaaagc attaagaaaa ttgcgccatc cttctcgttc aaaacaatta
    19081 aaagatttct tagaataagc gtggactatt gaccagaaga gaaatcagta aaactgtttg
    19141 gtttatttta aaagaaaaaa agcttgagac tacgcagtct caagcttttt tttctatagg
    19201 aaggaaattc attcattatt tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat
    19261 agaatatgat agattaaatg gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg
    19321 aaaaaatgtc caaattgcgg atcgaaaaaa atggagcaga atactatctg tccagactgc
    19381 ggtcatcaaa taactgcaga agatcagcaa ataaaagaat caattgaaga aacaaataca
    19441 gatgcagcta tgcaggataa atcaactgaa gatgcagtct ttcctgatac agaattaaac
    19501 gatccaatcg aatggtcgga attgaaagat ttacctctag aatctgtaat ggaattattc
    19561 gaaaaagcag aaccagaaga cgaagaacca cctgctcacg atcaagtaac taatatagag
    19621 aaaaatgaac agaagaaagc aactgttcat gagacttcgg aagaatcagg aaagaatcaa
    19681 gaagaaacgc ttgaatcaaa acaaaaaaga agagtagctg aactgaaaga aatcgtcgat
    19741 aatgaggaag aaaattcgat actatccgct tatatcaaag cacatcgtga agatacaaaa
    19801 gaagagcacg cgaaagagct gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa
    19861 gtagagccag aaaaggaaca agaaactgaa aaagactctg atagagaaaa cgccacaggt
    19921 aaagaaacag taaaaataaa agacgtaaaa acggatatca cagaaaaaac tgaagtattt
    19981 actgaaaaac aattagaaaa ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa
    20041 atactcgaaa acacagacaa aaagtcaaca gagcccgatg aaataaatag tgaatccgat
    20101 gctgaaaatc aatctgtaag caaagagggt actgatcttg aagcaaaaat agaaaaagaa
    20161 gagaaccagc ctaagctgtt tgaagcagaa gcgaaatcaa taacaatcgt gccagatact
    20221 gaaaaaacac atcaagaatc aaacttagaa aaaacagatc cagataaagc gcaaaagaaa
    20281 aaatcaaaaa aagtacctta tctcgtttta gcagcagtac ttctactcgg tggaggaagc
    20341 tgggcttatt atgaccatca tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga
    20401 cagcaacaaa tggaagaact tcatgagaaa cttgctgcct tttatacaga tgatcaccat
    20461 caatttattc gtgcagatat gattcatcaa gatttatcaa aactaaaaaa tgatttatcc
    20521 agtcttaaga atgaaaaagg atattcagag ttagaaacaa cttaccaaga tatccaatct
    20581 aagatccgag atatccaaaa agtgaatgag tggttcatag cacctgccat cgtcgatgat
    20641 catctagcag atgacatcaa gttgaaagca gataaagaga tccaagcaat cgaaacggat
    20701 aacacagatt ttggaaaatt gattcaacaa gcacaaacag aagctaaaaa tcaatatgct
    20761 gaattgcaaa cagctaaaga aaaaacagct atagtcttta aagacggaaa agtagtggat
    20821 tcagcaacac gcgaccaata caagacggca aaagaagcag tagaaaaagt gaaaaataca
    20881 gatctatctg ctcagttgaa tgaacaattg aaacaagtag ataagatgct cactgataaa
    20941 gagaaaaaag aagaagagga aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa
    21001 caagcaaaac aagccgctca agcagcagct gcagctcaag ccgctcaaca agctgcggtc
    21061 gcacaagcag cacagcaaga agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat
    21121 cagccaatca aatctgccag agcaagtgat attgccgatg catctaatcc agcatggacg
    21181 tgggcaccag gtgtccaaag caaagtgatt gctacatgta tccaacgtgg atatatcgtt
    21241 gaaggaggtt acagattaga aaaagccaga atcgaaaatg gggaaggcta ttataatcta
    21301 tatgccactt caacaaaatc tgcattgatg aaaggcattg gagaaagtgc tttgccattt
    21361 tacatcgtaa ccatcaactg taaaactgga tggttcggag gaaatgggag caattaattt
    21421 taggcaaatg aaagaggctg tgagcctgcc gtttaacacc gtttattcga aaaaggggag
    21481 tatgacacaa ctgttgtcat actccttttt tcacctttcc aaaccaaggc tcagctattc
    21541 aagtagataa cattatgtta tgatagcaaa gaaatgtatt aagaaaacct ttcactatag
    21601 agggagaaaa aaatgaacga tttattagca actatattta caggtattat tattgatgaa
    21661 aatgaaaaaa actactttgt ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa
    21721 ggtgaacaca aaatcggtga agcagttgaa ggattcggtt accaaaatca aaaacatgaa
    21781 aatcggatca ctacacaaat tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt
    21841 actgctacac gaaaagattt aggtgcgttt gtcgatatcg gtcttccaga taaagacttt
    21901 gtcgtatctc ttgatgaatt gccgacaatg acagagctat ggcctaaaaa aggggatcgc
    21961 ttgatgattt cccttagagt tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa
    22021 aaaatattca aatctttaag caaatatgga acagaagaaa tgaaaaacaa agatgtgata
    22081 ggaacagcat accgcttgaa attaacaggt acgtacgttt taacggaaga tttctatctt
    22141 gggtttatcc atccttctga acgatataat gaacctcgtc taggcgagca gataaaaggt
    22201 cgagtgattg gtgtccgacc agacggtgta ctgaatcttt cattgaagcc aagagcatat
    22261 gaagctatat cagatgatgc acaaatgatt ttaaccattt tagaacgttc tgcagatcag
    22321 caaatcaact ttacagataa atcggatcca gaagaaatca tgaaagcttt tggtatcagc
    22381 aaaggagcat ttaagcgcgc attaggtaat cttttgaaac aaggattgat tacacaagaa
    22441 aacggtgtga caaaactagt caaaaaatct aattgagaat cccttgcatg tttttcttaa
    22501 ctagcttata ataattttaa ttaacatagt gttatatctt tttttatttg taattattat
    22561 aaattgttgg ggttgagtaa atggtcatga atgctatttc ggcgatgaaa aaaacaaaac
    22621 agcagctaca tgaagcgggg tttaagctga caccgcaaag agaagctact ctgcttgtgc
    22681 ttttggaaaa tgagaaagag catttatctg cagaagaaat cttcttttta gtgaaaaaaa
    22741 agagtccgga aattggactt gctactgttt atcgaaccct tgaaatcttg acagaattga
    22801 aaattgtcga taaagtaagt tttaatgatg gattagcccg ttatgatttg agaaaagaag
    22861 gcgccaaaca tttccatcat catttactat gcttgaactg cggcacgatc gaggaagtag
    22921 aagaggatct tttacttgaa gtggagcaag tagtagaaga acgttatcat tttttagtaa
    22981 aagatcatcg gctaacgttc catggaatct gtcaggaatg ctatgaaaaa gaaa
//
LOCUS       DAJSNV010000041        22992 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSNV010000041 DAJSNV010000000
VERSION     DAJSNV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.042"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(110..565)
                     /locus_tag="ON516_001474"
     CDS             complement(110..565)
                     /locus_tag="ON516_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344966.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ON516_001475"
     CDS             complement(719..1927)
                     /locus_tag="ON516_001475"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3344967.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ON516_001476"
     CDS             complement(2039..2371)
                     /locus_tag="ON516_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3344968.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ON516_001477"
     CDS             2519..3394
                     /locus_tag="ON516_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3344969.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ON516_001478"
     CDS             complement(3476..4057)
                     /locus_tag="ON516_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3344970.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ON516_001479"
     CDS             complement(4063..5322)
                     /locus_tag="ON516_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3344971.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ON516_001480"
     CDS             complement(5324..6370)
                     /locus_tag="ON516_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3344972.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ON516_001481"
     CDS             complement(6605..6880)
                     /locus_tag="ON516_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3344973.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ON516_001482"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ON516_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3344974.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ON516_001483"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ON516_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3344975.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="ON516_001484"
     CDS             complement(9979..10659)
                     /locus_tag="ON516_001484"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3344976.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ON516_001485"
     CDS             complement(10708..11343)
                     /locus_tag="ON516_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3344977.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ON516_001486"
     CDS             complement(11409..12812)
                     /locus_tag="ON516_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3344978.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ON516_001487"
     CDS             complement(12799..13830)
                     /locus_tag="ON516_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3344979.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ON516_001488"
     CDS             13861..14082
                     /locus_tag="ON516_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3344980.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ON516_001489"
     CDS             complement(14247..14984)
                     /locus_tag="ON516_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3344981.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ON516_001490"
     CDS             complement(15137..16477)
                     /locus_tag="ON516_001490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3344982.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ON516_001491"
     CDS             complement(16714..18237)
                     /locus_tag="ON516_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3344983.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="ON516_001492"
     CDS             complement(18615..19226)
                     /locus_tag="ON516_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3344984.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="ON516_001493"
     CDS             complement(19556..20272)
                     /locus_tag="ON516_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3344985.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ON516_001494"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ON516_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3344986.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="ON516_001495"
     CDS             complement(20831..21628)
                     /locus_tag="ON516_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3344987.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="ON516_001496"
     CDS             complement(21598..21969)
                     /locus_tag="ON516_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3344988.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ON516_001497"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ON516_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3344989.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6447 a         4913 c         3899 g         7733 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tt
//
LOCUS       DAJSNV010000042        22259 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSNV010000042 DAJSNV010000000
VERSION     DAJSNV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.043"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            154..396
                     /gene="yaaA"
                     /locus_tag="ON516_001498"
     CDS             154..396
                     /gene="yaaA"
                     /locus_tag="ON516_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3344990.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            383..1507
                     /gene="recF"
                     /locus_tag="ON516_001499"
     CDS             383..1507
                     /gene="recF"
                     /locus_tag="ON516_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3344991.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1504..3450
                     /gene="gyrB"
                     /locus_tag="ON516_001500"
     CDS             1504..3450
                     /gene="gyrB"
                     /locus_tag="ON516_001500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3344992.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3470..5941
                     /gene="gyrA"
                     /locus_tag="ON516_001501"
     CDS             3470..5941
                     /gene="gyrA"
                     /locus_tag="ON516_001501"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3344993.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6177..6476
                     /locus_tag="ON516_001502"
     CDS             6177..6476
                     /locus_tag="ON516_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3344994.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6526..7038
                     /gene="ssb"
                     /locus_tag="ON516_001503"
     CDS             6526..7038
                     /gene="ssb"
                     /locus_tag="ON516_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3344995.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7064..7300
                     /locus_tag="ON516_001504"
     CDS             7064..7300
                     /locus_tag="ON516_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3344996.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7443..9416
                     /locus_tag="ON516_001505"
     CDS             7443..9416
                     /locus_tag="ON516_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3344997.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9422..9874
                     /gene="rplI"
                     /locus_tag="ON516_001506"
     CDS             9422..9874
                     /gene="rplI"
                     /locus_tag="ON516_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3344998.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10148..11515
                     /gene="dnaB"
                     /locus_tag="ON516_001507"
     CDS             10148..11515
                     /gene="dnaB"
                     /locus_tag="ON516_001507"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3344999.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12295..13587
                     /locus_tag="ON516_001508"
     CDS             12295..13587
                     /locus_tag="ON516_001508"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3345000.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14374..15237
                     /locus_tag="ON516_001509"
     CDS             14374..15237
                     /locus_tag="ON516_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3345001.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15366..16988)
                     /locus_tag="ON516_001510"
     CDS             complement(15366..16988)
                     /locus_tag="ON516_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3345002.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17237..17758
                     /locus_tag="ON516_001511"
     CDS             17237..17758
                     /locus_tag="ON516_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3345003.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17944..19674)
                     /gene="spxB"
                     /locus_tag="ON516_001512"
     CDS             complement(17944..19674)
                     /gene="spxB"
                     /locus_tag="ON516_001512"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3345004.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19839..20657
                     /locus_tag="ON516_001513"
     CDS             19839..20657
                     /locus_tag="ON516_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3345005.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20760..20957)
                     /locus_tag="ON516_001514"
     CDS             complement(20760..20957)
                     /locus_tag="ON516_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345006.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21126..21704
                     /locus_tag="ON516_001515"
     CDS             21126..21704
                     /locus_tag="ON516_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3345007.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21799..21936
                     /locus_tag="ON516_001516"
     CDS             21799..21936
                     /locus_tag="ON516_001516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345008.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7143 a         4006 c         4689 g         6421 t
ORIGIN      
        1 aatctctttt aaggctttct gtttattttt gtttaaatca ccttttttag attaaatcgt
       61 cttatatgga aaaatatatg gataaatttg ttttattgcg aaaaaaaagg tataatagag
      121 tatactttta ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg
      181 gaattcatga cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa
      241 gcaaaatggt atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt
      301 ggacgcaaac tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg
      361 gtaaaaaaag gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa
      421 ttacgaagag ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc
      481 gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg
      541 gacaagtagt gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga
      601 gatcacaaaa ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa
      661 aaccaagatc aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt
      721 cattttattt gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa
      781 gtttttagat atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata
      841 ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac
      901 tgatttgctt tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt
      961 gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca
     1021 aatcacccat caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga
     1081 aacacatacg caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa
     1141 gaaagacctt tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt
     1201 tattaatcag aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact
     1261 aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct
     1321 tttgttagat gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac
     1381 aatcgaagga aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa
     1441 aatgactgtt gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac
     1501 tgaatgacag aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt
     1561 caagtacttg aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact
     1621 agttctgagg gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta
     1681 ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt
     1741 attgacgacg gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt
     1801 gaaacggtct ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt
     1861 tctggtggac ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat
     1921 gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg
     1981 gacgacttaa agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca
     2041 gaccctgaaa tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc
     2101 cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa
     2161 gaaccagtca tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg
     2221 aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat
     2281 atcgcagttg aagtctctat gcaatataca gacggctacc attccaatat catgagtttt
     2341 gccaacaata tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta
     2401 actcgtgtga taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac
     2461 ttgaccggtg aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac
     2521 cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact
     2581 gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg
     2641 caaattgttg aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt
     2701 gaagtaacga gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac
     2761 tgctctagca acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc
     2821 ggatcagcta agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa
     2881 atcttgaatg ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct
     2941 ttatttactg ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac
     3001 cacaaattag ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg
     3061 ctgactcttt tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct
     3121 cagccacctt tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa
     3181 aatgcagaag aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc
     3241 gttcagcgtt ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg
     3301 gacccaagca atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag
     3361 atatttgaaa tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat
     3421 gcacactatg tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga
     3481 aatcagagaa aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga
     3541 ttatgcgatg agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc
     3601 cgttcatcgc cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca
     3661 taaaaaatct gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat
     3721 tgcgatctat gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt
     3781 ggatggtcat ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac
     3841 cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt
     3901 tgatttccaa agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt
     3961 cccgaatctt ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc
     4021 acctcataac ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt
     4081 gacgaccaat gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt
     4141 tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg
     4201 tgcaaaagtt gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt
     4261 gccttatatg gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa
     4321 gcgtatcgaa gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt
     4381 cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac
     4441 atccttgcaa aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt
     4501 tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg
     4561 acggacggtc tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg
     4621 tattgctttg gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga
     4681 cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat
     4741 tttagatatg cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata
     4801 ccaagaactc ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt
     4861 tacagaaatc atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg
     4921 cacagaatta ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga
     4981 agaaatcgtt attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt
     5041 cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt
     5101 cgtcaaaaat ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg
     5161 aaaagtgtat cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat
     5221 cccaatcatc aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt
     5281 gacgggagaa gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa
     5341 acgtacttct gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt
     5401 gaaagaagac gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat
     5461 tggaacacat aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg
     5521 tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc
     5581 cttaatgaag gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac
     5641 aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat
     5701 cactgaaaaa aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat
     5761 gctgattaca gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg
     5821 acgttcaaca ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat
     5881 ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata
     5941 accatcaaga ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc
     6001 tgctggcttt tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat
     6061 tgcttttgac caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata
     6121 actctccttg ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg
     6181 aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa
     6241 ctgctttaat cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat
     6301 caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct
     6361 atcacatcgt taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc
     6421 ttgctaaaat caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt
     6481 tgtttcatgt gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt
     6541 gtattagttg gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca
     6601 gtcgcaacat ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag
     6661 gctgatttta tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct
     6721 cgtaaaggaa cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag
     6781 caaggacaac gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt
     6841 cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct
     6901 ggcggatttg gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct
     6961 gatttcgacc gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac
     7021 gatgatttac cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag
     7081 aggcggacgt aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga
     7141 ttataaagat atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg
     7201 tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc
     7261 tcgtatcatg ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg
     7321 actgatcctt ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt
     7381 ttagtcgctc gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa
     7441 ttatggaaaa taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat
     7501 taatcgaagt attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc
     7561 tattgatgaa tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa
     7621 ataaacgtga aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct
     7681 cagaagtcat gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc
     7741 tcaatccatt agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag
     7801 atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat
     7861 ttcagtttga tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc
     7921 agttgttaaa actccatcag acgaacagtc agccggtgat tggtatttta tctattgata
     7981 actatgcaga tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg
     8041 tcactacact agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg
     8101 cagaacggtt tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat
     8161 ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta
     8221 gtatggggat tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata
     8281 atttagatgt tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag
     8341 gagcaaaacc tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc
     8401 gttctcgagc gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta
     8461 tcatggggca caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc
     8521 tggcacgctt caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag
     8581 atgtagaacg gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct
     8641 ctccagaacg tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc
     8701 ataaaccttc tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca
     8761 ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat
     8821 cttcagcttc ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa
     8881 caaatcgctt agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa
     8941 atagtttcaa tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt
     9001 gtggagcaga cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg
     9061 agatcagcaa tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag
     9121 ggacagacga aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt
     9181 cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga
     9241 tcagtgccag aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg
     9301 gaggacactt cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac
     9361 agaagctatt tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca
     9421 aatgaaagta atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga
     9481 agttccagca ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac
     9541 gaaaagcagt atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga
     9601 aatcttggaa gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga
     9661 attgaaggcg aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat
     9721 tgctgaaggg ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga
     9781 aaatccgatc cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac
     9841 ggctacgatc aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg
     9901 gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc
     9961 aatgaaagca aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag
    10021 caaaatagca taaagaaaac gagtataata aaagagaact atttttttat tacactttcg
    10081 gtacgaatga tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc
    10141 gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca
    10201 agcggtttta ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat
    10261 agagccaaaa gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt
    10321 aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa
    10381 tttgttagaa gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac
    10441 agcagcaaat atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt
    10501 gatccagaca gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac
    10561 aattttagat gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg
    10621 ctttttatct attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca
    10681 aaacaatgaa gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc
    10741 aggtttccag cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc
    10801 gtttgcattg aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt
    10861 tagtttggaa atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat
    10921 tgaggccagc catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt
    10981 tgcgatgggg agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat
    11041 ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat
    11101 attgatcgat tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt
    11161 atcagatatc tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc
    11221 tctttcacag ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga
    11281 tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga
    11341 tgattattat gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa
    11401 cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact
    11461 gtttatcaaa gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag
    11521 aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca
    11581 ggaacgacta gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt
    11641 cacagtccct ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc
    11701 aaacgtcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    11761 ctgatcttac gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
    11821 cacaatatca tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta
    11881 aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
    11941 cgcccaatca aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
    12001 gcaggacagc tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa
    12061 ataagtcgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt
    12121 gaagctgacc acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc
    12181 gttttttaag cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt
    12241 atctattatt gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca
    12301 tctgtagtag tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt
    12361 ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca
    12421 attaagttta acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa
    12481 gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa
    12541 ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg
    12601 cacgtaatct tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa
    12661 aataagattg gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt
    12721 gtaggtatcc gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg
    12781 aatctagatg acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt
    12841 gacgatattt ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat
    12901 acttccgtta tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca
    12961 caaggtgtaa tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca
    13021 gtagccggtg gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc
    13081 ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt
    13141 gatgaaacag gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt
    13201 ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt
    13261 attaccaacc tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt
    13321 tgtacggctt atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag
    13381 ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga
    13441 tgcaaaacac ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag
    13501 cttgttggtg tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc
    13561 ttagaaagcg tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt
    13621 accagagcga gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag
    13681 agacgtttag ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg
    13741 actgtgacaa aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc
    13801 atctacggca ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt
    13861 cttactgctt gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc
    13921 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
    13981 ttccttgtga ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc
    14041 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt
    14101 ttttctctaa tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag
    14161 aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    14221 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac
    14281 ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata
    14341 agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt
    14401 tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc
    14461 caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac
    14521 atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat
    14581 ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc
    14641 agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa
    14701 tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt
    14761 ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag
    14821 tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat
    14881 ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta
    14941 aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga
    15001 agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt
    15061 gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg
    15121 cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc
    15181 gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag
    15241 cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa
    15301 aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata
    15361 ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca
    15421 tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt
    15481 gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc
    15541 atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct
    15601 aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct
    15661 ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct
    15721 aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag
    15781 tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta
    15841 cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc catgataact
    15901 ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg
    15961 ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt
    16021 tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa
    16081 cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa
    16141 gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct
    16201 ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta
    16261 gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc
    16321 gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta
    16381 ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt
    16441 gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact
    16501 tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg
    16561 ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca
    16621 aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg
    16681 taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt
    16741 tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca
    16801 tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg
    16861 aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat
    16921 ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca
    16981 gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat
    17041 ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag
    17101 aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg
    17161 caacagcata atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat
    17221 gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg
    17281 aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc
    17341 aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg
    17401 gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat
    17461 cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc
    17521 ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt
    17581 ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat
    17641 tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt
    17701 agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt
    17761 aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc
    17821 cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta
    17881 tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag
    17941 ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt
    18001 ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc
    18061 tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt
    18121 tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc
    18181 cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt
    18241 ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac
    18301 attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa
    18361 cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat
    18421 tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt
    18481 accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa
    18541 aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg
    18601 ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg
    18661 atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa
    18721 agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga
    18781 cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg
    18841 gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt
    18901 agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt
    18961 tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg
    19021 aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata
    19081 aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg
    19141 agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac
    19201 gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag
    19261 actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc
    19321 atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc
    19381 tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc
    19441 aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc
    19501 tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg
    19561 atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat
    19621 accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt
    19681 tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat
    19741 cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct
    19801 agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac
    19861 ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg
    19921 gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag
    19981 gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta
    20041 caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag
    20101 gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac
    20161 atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta
    20221 cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg
    20281 cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc
    20341 aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta
    20401 aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg
    20461 gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag
    20521 gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca
    20581 gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg
    20641 atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt
    20701 atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat
    20761 taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt
    20821 gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa
    20881 agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac
    20941 atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat
    21001 aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa
    21061 tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga
    21121 atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga
    21181 ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat
    21241 taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca
    21301 atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca
    21361 gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca
    21421 tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt
    21481 attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg
    21541 cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg
    21601 tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg
    21661 cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa
    21721 aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt
    21781 attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt
    21841 gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta
    21901 ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc
    21961 tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt
    22021 tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag
    22081 tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct
    22141 tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa
    22201 acggtgtggt ttttggaata aaaaaataac atatctcatt ttacactttt gttatttta
//
LOCUS       DAJSNV010000043        22220 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSNV010000043 DAJSNV010000000
VERSION     DAJSNV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.044"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            53..1951
                     /locus_tag="ON516_001517"
     CDS             53..1951
                     /locus_tag="ON516_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3345009.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2281..3000)
                     /locus_tag="ON516_001518"
     CDS             complement(2281..3000)
                     /locus_tag="ON516_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3345010.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3337..3669
                     /locus_tag="ON516_001519"
     CDS             3337..3669
                     /locus_tag="ON516_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345011.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3656..5659
                     /locus_tag="ON516_001520"
     CDS             3656..5659
                     /locus_tag="ON516_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3345012.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5678..6148
                     /locus_tag="ON516_001521"
     CDS             5678..6148
                     /locus_tag="ON516_001521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3345013.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6169..6753
                     /locus_tag="ON516_001522"
     CDS             6169..6753
                     /locus_tag="ON516_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3345014.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6768..7754
                     /locus_tag="ON516_001523"
     CDS             6768..7754
                     /locus_tag="ON516_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3345015.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7744..8055
                     /locus_tag="ON516_001524"
     CDS             7744..8055
                     /locus_tag="ON516_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3345016.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8077..9858
                     /locus_tag="ON516_001525"
     CDS             8077..9858
                     /locus_tag="ON516_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3345017.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9851..11227
                     /locus_tag="ON516_001526"
     CDS             9851..11227
                     /locus_tag="ON516_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3345018.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11234..11869
                     /locus_tag="ON516_001527"
     CDS             11234..11869
                     /locus_tag="ON516_001527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3345019.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11899..13254
                     /locus_tag="ON516_001528"
     CDS             11899..13254
                     /locus_tag="ON516_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3345020.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13446..14840)
                     /locus_tag="ON516_001529"
     CDS             complement(13446..14840)
                     /locus_tag="ON516_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3345021.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14932..15903
                     /locus_tag="ON516_001530"
     CDS             14932..15903
                     /locus_tag="ON516_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3345022.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15900..16682
                     /locus_tag="ON516_001531"
     CDS             15900..16682
                     /locus_tag="ON516_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3345023.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16851..17222)
                     /locus_tag="ON516_001532"
     CDS             complement(16851..17222)
                     /locus_tag="ON516_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3345024.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17356..17799
                     /locus_tag="ON516_001533"
     CDS             17356..17799
                     /locus_tag="ON516_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3345025.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="ON516_001534"
     CDS             complement(17842..18651)
                     /gene="yidA"
                     /locus_tag="ON516_001534"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3345026.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18670..20037)
                     /locus_tag="ON516_001535"
     CDS             complement(18670..20037)
                     /locus_tag="ON516_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3345027.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20021..20851)
                     /locus_tag="ON516_001536"
     CDS             complement(20021..20851)
                     /locus_tag="ON516_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3345028.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21014..21451
                     /locus_tag="ON516_001537"
     CDS             21014..21451
                     /locus_tag="ON516_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3345029.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21527..22147
                     /locus_tag="ON516_001538"
     CDS             21527..22147
                     /locus_tag="ON516_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3345030.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7125 a         3837 c         4790 g         6468 t
ORIGIN      
        1 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
       61 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
      121 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
      181 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
      241 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
      301 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
      361 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
      421 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
      481 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
      541 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
      601 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
      661 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
      721 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
      781 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
      841 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
      901 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
      961 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
     1021 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
     1081 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
     1141 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
     1201 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
     1261 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
     1321 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
     1381 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
     1441 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
     1501 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
     1561 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
     1621 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
     1681 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
     1741 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
     1801 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
     1861 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
     1921 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
     1981 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
     2041 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
     2101 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
     2161 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
     2221 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
     2281 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
     2341 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
     2401 ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
     2461 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
     2521 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
     2581 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
     2641 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
     2701 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
     2761 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
     2821 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
     2881 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
     2941 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
     3001 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
     3061 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
     3121 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
     3181 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
     3241 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
     3301 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
     3361 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
     3421 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
     3481 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
     3541 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
     3601 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
     3661 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
     3721 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
     3781 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
     3841 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
     3901 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
     3961 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
     4021 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
     4081 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
     4141 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
     4201 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
     4261 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
     4321 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
     4381 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
     4441 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
     4501 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
     4561 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
     4621 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
     4681 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
     4741 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
     4801 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
     4861 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
     4921 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
     4981 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
     5041 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
     5101 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
     5161 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
     5221 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
     5281 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
     5341 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
     5401 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
     5461 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
     5521 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
     5581 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
     5641 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
     5701 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
     5761 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
     5821 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc aaggtttgta
     5881 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
     5941 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
     6001 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
     6061 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
     6121 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
     6181 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
     6241 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
     6301 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
     6361 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
     6421 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
     6481 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
     6541 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
     6601 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
     6661 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
     6721 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
     6781 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
     6841 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
     6901 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
     6961 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
     7021 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
     7081 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
     7141 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
     7201 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
     7261 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
     7321 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
     7381 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
     7441 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
     7501 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
     7561 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
     7621 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
     7681 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
     7741 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
     7801 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
     7861 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
     7921 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
     7981 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
     8041 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
     8101 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
     8161 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
     8221 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
     8281 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
     8341 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
     8401 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
     8461 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
     8521 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
     8581 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
     8641 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
     8701 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
     8761 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
     8821 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
     8881 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
     8941 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
     9001 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
     9061 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
     9121 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
     9181 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
     9241 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
     9301 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
     9361 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
     9421 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
     9481 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
     9541 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
     9601 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
     9661 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
     9721 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
     9781 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
     9841 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
     9901 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
     9961 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    10021 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    10081 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    10141 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    10201 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    10261 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    10321 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    10381 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    10441 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    10501 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    10561 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    10621 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    10681 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    10741 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    10801 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    10861 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    10921 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    10981 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    11041 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    11101 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    11161 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    11221 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    11281 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    11341 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    11401 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    11461 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    11521 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    11581 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    11641 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    11701 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    11761 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    11821 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    11881 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    11941 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    12001 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    12061 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    12121 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    12181 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    12241 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    12301 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    12361 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    12421 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    12481 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    12541 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    12601 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    12661 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    12721 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    12781 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    12841 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    12901 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    12961 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    13021 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    13081 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    13141 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    13201 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    13261 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    13321 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    13381 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    13441 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    13501 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgcacgtgta
    13561 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    13621 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    13681 ttttctactt tgaaaagaga atcaaaaagc tgattggatc tttttgtaag acttaattta
    13741 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    13801 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    13861 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    13921 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    13981 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    14041 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    14101 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    14161 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    14221 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    14281 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    14341 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    14401 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    14461 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    14521 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    14581 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    14641 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    14701 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    14761 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    14821 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    14881 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    14941 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    15001 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    15061 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    15121 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    15181 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    15241 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    15301 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    15361 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    15421 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    15481 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    15541 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    15601 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    15661 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    15721 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    15781 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    15841 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    15901 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    15961 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    16021 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    16081 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    16141 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    16201 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    16261 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    16321 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    16381 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    16441 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    16501 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    16561 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    16621 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    16681 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    16741 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    16801 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    16861 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    16921 ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt
    16981 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    17041 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    17101 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    17161 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    17221 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    17281 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    17341 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    17401 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    17461 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    17521 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    17581 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    17641 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    17701 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    17761 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    17821 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    17881 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    17941 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    18001 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    18061 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    18121 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    18181 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    18241 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    18301 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccattac
    18361 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    18421 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    18481 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    18541 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    18601 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    18661 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    18721 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    18781 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    18841 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    18901 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    18961 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    19021 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    19081 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    19141 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    19201 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    19261 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    19321 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    19381 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    19441 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    19501 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    19561 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    19621 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    19681 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    19741 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    19801 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    19861 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    19921 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    19981 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    20041 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    20101 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    20161 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    20221 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    20281 cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt tcctgattac
    20341 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    20401 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    20461 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    20521 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    20581 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    20641 aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    20701 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    20761 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    20821 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    20881 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    20941 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    21001 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    21061 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    21121 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    21181 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    21241 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    21301 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    21361 cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    21421 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    21481 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    21541 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    21601 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    21661 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    21721 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    21781 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    21841 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    21901 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    21961 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    22021 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    22081 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    22141 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    22201 ggaggtgtga cacgattttt
//
LOCUS       DAJSNV010000044        22183 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSNV010000044 DAJSNV010000000
VERSION     DAJSNV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.045"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(104..622)
                     /locus_tag="ON516_001539"
     CDS             complement(104..622)
                     /locus_tag="ON516_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3345031.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(624..1241)
                     /locus_tag="ON516_001540"
     CDS             complement(624..1241)
                     /locus_tag="ON516_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3345032.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1238..2092)
                     /locus_tag="ON516_001541"
     CDS             complement(1238..2092)
                     /locus_tag="ON516_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3345033.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2089..3000)
                     /locus_tag="ON516_001542"
     CDS             complement(2089..3000)
                     /locus_tag="ON516_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3345034.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3041..4375)
                     /locus_tag="ON516_001543"
     CDS             complement(3041..4375)
                     /locus_tag="ON516_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3345035.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4583..5413
                     /locus_tag="ON516_001544"
     CDS             4583..5413
                     /locus_tag="ON516_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3345036.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5657..5975)
                     /locus_tag="ON516_001545"
                     /pseudo
     CDS             complement(5657..5975)
                     /locus_tag="ON516_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..6291)
                     /locus_tag="ON516_001546"
     CDS             complement(6097..6291)
                     /locus_tag="ON516_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345037.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6796..6981)
                     /locus_tag="ON516_001547"
     CDS             complement(6796..6981)
                     /locus_tag="ON516_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345038.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7831..8172)
                     /locus_tag="ON516_001548"
     CDS             complement(7831..8172)
                     /locus_tag="ON516_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3345039.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8172..8402)
                     /locus_tag="ON516_001549"
     CDS             complement(8172..8402)
                     /locus_tag="ON516_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3345040.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8689..9027
                     /locus_tag="ON516_001550"
     CDS             8689..9027
                     /locus_tag="ON516_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345041.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9232..9807)
                     /locus_tag="ON516_001551"
     CDS             complement(9232..9807)
                     /locus_tag="ON516_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3345042.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(10416..10997)
                     /locus_tag="ON516_001552"
     CDS             complement(10416..10997)
                     /locus_tag="ON516_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3345043.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11180..11806)
                     /locus_tag="ON516_001553"
     CDS             complement(11180..11806)
                     /locus_tag="ON516_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3345044.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11828..13159)
                     /locus_tag="ON516_001554"
     CDS             complement(11828..13159)
                     /locus_tag="ON516_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3345045.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13314..13715)
                     /locus_tag="ON516_001555"
     CDS             complement(13314..13715)
                     /locus_tag="ON516_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3345046.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13801..14007)
                     /locus_tag="ON516_001556"
     CDS             complement(13801..14007)
                     /locus_tag="ON516_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345047.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14102..15604)
                     /locus_tag="ON516_001557"
     CDS             complement(14102..15604)
                     /locus_tag="ON516_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3345048.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15622..16077)
                     /gene="argR"
                     /locus_tag="ON516_001558"
     CDS             complement(15622..16077)
                     /gene="argR"
                     /locus_tag="ON516_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3345049.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16175..16792)
                     /locus_tag="ON516_001559"
     CDS             complement(16175..16792)
                     /locus_tag="ON516_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3345050.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17013..18386
                     /locus_tag="ON516_001560"
     CDS             17013..18386
                     /locus_tag="ON516_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3345051.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18461..19573
                     /locus_tag="ON516_001561"
     CDS             18461..19573
                     /locus_tag="ON516_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3345052.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(19645..21283)
                     /locus_tag="ON516_001562"
                     /pseudo
     CDS             complement(19645..21283)
                     /locus_tag="ON516_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
     gene            complement(21451..22059)
                     /locus_tag="ON516_001563"
     CDS             complement(21451..22059)
                     /locus_tag="ON516_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3345053.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6475 a         4511 c         3867 g         7330 t
ORIGIN      
        1 taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa aaataaagca
       61 caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata acggcagcgc
      121 ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta ggataatgta
      181 aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct aatattcctg
      241 caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct gtatgtcctg
      301 aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga tttttttcat
      361 aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa aaatagatca
      421 aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag actaaaacag
      481 cagcaaggag taaccaaata aaaccaccat tagagatatt actgataaat ttcaaaaaat
      541 tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata ctatcatcga
      601 ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc ttgcgtacga
      661 tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa agcaaaatag
      721 attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa atagaaaagc
      781 gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc ttctttttgt
      841 tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag caaaaacaac
      901 gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat ctgataatcg
      961 atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata aagagagaag
     1021 ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt ttttttgtaa
     1081 tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac aggcaagacg
     1141 cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat gatttctaag
     1201 aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa cctcctgtca
     1261 aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc gtcgggatca
     1321 agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg ttctgaaaaa
     1381 cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac agaggaccta
     1441 agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag gcaggtttcg
     1501 tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg atttcagcag
     1561 cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat atattgaacg
     1621 cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag ccgatcttgg
     1681 agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc gtagaaagaa
     1741 ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca taagcaataa
     1801 aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat gtatttcttg
     1861 catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat acgatttttt
     1921 ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat gaagtaaaca
     1981 ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg acataactta
     2041 gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc atttgccgtc
     2101 acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg tcagaaccaa
     2161 cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa aagctacgaa
     2221 cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc cgcctgttcc
     2281 atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg tagaggtgat
     2341 cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga actttcccca
     2401 aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat tttgtaatgt
     2461 agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa tcaacgcagg
     2521 tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc agcccagaaa
     2581 tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt agactgctac
     2641 tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc ctttgatttt
     2701 ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta cattgaagat
     2761 gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa cgaacattct
     2821 tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat cggtgaaact
     2881 accaataact gaaaaaagca taggccctag cataaatacc aagaacccga taataaaagg
     2941 cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata aacgcttcaa
     3001 attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt tgcttgacta
     3061 agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt gttcctaaaa
     3121 gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct aaaggaactg
     3181 gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt tttcctttat
     3241 ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc atgatatcaa
     3301 aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg atgaattcat
     3361 aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg ccaccccacg
     3421 accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa tcaaaatcat
     3481 ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc atggctacta
     3541 atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat ttattgatcg
     3601 tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc actttatttt
     3661 tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta tctgcgttta
     3721 gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt gtaaattctt
     3781 ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa gttgtcggta
     3841 gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa atgacttgtg
     3901 tatatgtatt gaatgggatc gcataaggat caccttcata ttctactgta tcccaaagct
     3961 gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta gttgcttgct
     4021 gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg ggtgcatttc
     4081 cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga tacttataat
     4141 ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg atagcttctt
     4201 ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca tcacttgttt
     4261 tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat ccgcatcctc
     4321 ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt ttcatatttt
     4381 acctccaaat tttaatgtaa ccgatgtcgg ttttaatata acgatatcat tttgttctga
     4441 aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa tactttatat
     4501 aattcgcaag cgttttcttt gaatttttta atctttcgta gcagaatgag aatgtattaa
     4561 aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt ttgaagcaaa
     4621 accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg gcggccaata
     4681 tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc gaggagaaga
     4741 aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg tggaaatgtt
     4801 ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag aagcagcgat
     4861 gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc acgcgatcgt
     4921 agctggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg gtgcctatat
     4981 cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg aatcacaagg
     5041 aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa aagatatgct
     5101 gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg cagtgaaaga
     5161 attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg acaaagaacg
     5221 aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta gtgaaggttc
     5281 ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga aagaacctaa
     5341 gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg ggacacctcc
     5401 attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca cgctttgtga
     5461 gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt ttctctagag
     5521 ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt ttatgttcaa
     5581 gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact actccttagc
     5641 ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta tcatgttttt
     5701 cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt gtcacatagt
     5761 atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca caatctcttg
     5821 atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct atactttggt
     5881 gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt tccctttatt
     5941 gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc aattaggtct
     6001 aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt tttcagaaaa
     6061 aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt ttttataaat
     6121 ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaaccagaac ctatgaaatc
     6181 tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata tatttatcag
     6241 aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca tagactctcc
     6301 tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat taaaggggcg
     6361 cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg tgtttaataa
     6421 ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg acctccaatt
     6481 tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc acttatatat
     6541 cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc tacttgcatc
     6601 taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact attaattttt
     6661 aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact ttctgtctca
     6721 tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc tggaatagtt
     6781 gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa atacaatgaa
     6841 aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca actcattact
     6901 gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga tggttaggaa
     6961 tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat tatagaaggt
     7021 attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt acattatact
     7081 ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc gtgtaatcgg
     7141 taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa
     7201 ggtactttta cattcataat tgtcgctttg agattctact ttggttcact atctgaccca
     7261 attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag
     7321 cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt
     7381 ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat
     7441 agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag
     7501 tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt
     7561 tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg
     7621 tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat
     7681 cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc
     7741 acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata
     7801 taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat
     7861 tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc
     7921 aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga
     7981 gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt
     8041 tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt
     8101 tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct
     8161 ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc
     8221 tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta
     8281 attgttccat catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg
     8341 ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc
     8401 attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc
     8461 tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa
     8521 ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac
     8581 ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca
     8641 gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat
     8701 aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa
     8761 ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca
     8821 ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct
     8881 atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa
     8941 cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt
     9001 agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac
     9061 tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta
     9121 tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt tgggatctgt
     9181 gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt
     9241 ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt
     9301 tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc
     9361 gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg
     9421 gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc
     9481 tttttcttca ttccactcat aaaagccact cattgggaaa acacaacgat tttgcaaaaa
     9541 tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg
     9601 tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc
     9661 ctcttttctt aacgtcacaa caagatttga cggaaaaact tccccagctg ctaattctgc
     9721 tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc
     9781 tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac
     9841 actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt
     9901 gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt
     9961 cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg
    10021 aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agcttgcatc tgcggtaata
    10081 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
    10141 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
    10201 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
    10261 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
    10321 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    10381 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
    10441 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
    10501 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
    10561 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
    10621 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
    10681 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
    10741 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
    10801 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
    10861 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
    10921 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
    10981 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
    11041 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
    11101 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
    11161 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
    11221 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
    11281 caattgcatc tgtcgctacc acaacattga atccactctc tatcaattca cgcatatggg
    11341 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
    11401 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
    11461 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
    11521 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
    11581 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
    11641 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
    11701 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
    11761 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
    11821 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
    11881 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
    11941 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
    12001 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
    12061 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
    12121 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
    12181 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
    12241 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
    12301 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
    12361 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
    12421 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
    12481 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
    12541 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
    12601 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
    12661 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
    12721 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
    12781 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
    12841 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
    12901 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
    12961 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
    13021 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
    13081 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
    13141 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
    13201 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
    13261 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
    13321 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
    13381 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
    13441 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
    13501 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
    13561 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
    13621 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
    13681 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
    13741 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
    13801 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
    13861 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
    13921 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
    13981 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
    14041 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
    14101 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
    14161 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
    14221 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
    14281 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
    14341 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
    14401 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
    14461 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
    14521 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
    14581 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
    14641 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
    14701 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
    14761 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
    14821 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
    14881 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
    14941 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
    15001 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
    15061 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
    15121 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
    15181 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
    15241 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
    15301 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
    15361 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
    15421 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
    15481 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
    15541 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
    15601 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
    15661 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
    15721 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
    15781 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
    15841 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
    15901 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
    15961 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
    16021 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
    16081 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
    16141 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
    16201 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
    16261 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
    16321 ttgcaggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
    16381 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
    16441 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
    16501 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
    16561 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
    16621 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
    16681 tcgggtcgcc gatccctctg atgctaaaga atggtttttt aggaacctct agtatttgcg
    16741 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
    16801 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
    16861 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
    16921 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
    16981 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
    17041 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
    17101 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
    17161 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
    17221 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
    17281 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
    17341 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
    17401 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
    17461 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
    17521 ttcttgtgcc cgttttgatt ctttctttga ttatcggctg gtttgctatt cccgagatgg
    17581 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
    17641 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
    17701 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
    17761 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
    17821 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
    17881 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
    17941 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
    18001 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
    18061 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
    18121 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
    18181 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
    18241 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
    18301 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    18361 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    18421 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    18481 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    18541 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    18601 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    18661 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    18721 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    18781 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    18841 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    18901 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    18961 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga
    19021 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    19081 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    19141 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    19201 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    19261 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    19321 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    19381 agatttttcc ccattttttg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    19441 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    19501 aaatactggt cattggtcta cggaagatta tcaaaaggac atctccacct atattacgaa
    19561 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    19621 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    19681 ttttgcttca tctagcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    19741 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    19801 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    19861 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    19921 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    19981 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    20041 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    20101 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    20161 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    20221 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    20281 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    20341 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    20401 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    20461 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    20521 tacagccatc gtataggtct tggtcattac tagcatattg tttcatccca ccgtggcagg
    20581 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    20641 ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga
    20701 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    20761 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    20821 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    20881 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    20941 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    21001 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    21061 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    21121 tcgttcctgg actccctttt gctagttgat aagctgtctt cctgtcaaaa acttcatgaa
    21181 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    21241 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    21301 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    21361 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    21421 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    21481 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    21541 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    21601 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    21661 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    21721 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    21781 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    21841 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    21901 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    21961 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    22021 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    22081 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    22141 aacatgacat ttaatgacaa aataacgaat tatttaatat ttg
//
LOCUS       DAJSNV010000045        21637 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSNV010000045 DAJSNV010000000
VERSION     DAJSNV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.046"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..398)
                     /locus_tag="ON516_001564"
     CDS             complement(<1..398)
                     /locus_tag="ON516_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCR3345054.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGA"
     gene            complement(485..1189)
                     /locus_tag="ON516_001565"
     CDS             complement(485..1189)
                     /locus_tag="ON516_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR3345055.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1389..1655
                     /locus_tag="ON516_001566"
     CDS             1389..1655
                     /locus_tag="ON516_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345056.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1668..1826
                     /locus_tag="ON516_001567"
     CDS             1668..1826
                     /locus_tag="ON516_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345057.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1823..2053)
                     /locus_tag="ON516_001568"
     CDS             complement(1823..2053)
                     /locus_tag="ON516_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3345058.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2125..2280
                     /locus_tag="ON516_001569"
     CDS             2125..2280
                     /locus_tag="ON516_001569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345059.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            2437..2592
                     /locus_tag="ON516_001570"
     CDS             2437..2592
                     /locus_tag="ON516_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345060.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2589..2813)
                     /locus_tag="ON516_001571"
     CDS             complement(2589..2813)
                     /locus_tag="ON516_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3345061.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3080..3415
                     /locus_tag="ON516_001572"
     CDS             3080..3415
                     /locus_tag="ON516_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345062.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3648..4589
                     /locus_tag="ON516_001573"
     CDS             3648..4589
                     /locus_tag="ON516_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCR3345063.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4591..5481
                     /locus_tag="ON516_001574"
     CDS             4591..5481
                     /locus_tag="ON516_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCR3345064.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5524..6324
                     /locus_tag="ON516_001575"
     CDS             5524..6324
                     /locus_tag="ON516_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3345065.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6339..7190
                     /locus_tag="ON516_001576"
     CDS             6339..7190
                     /locus_tag="ON516_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3345066.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7187..7504
                     /locus_tag="ON516_001577"
     CDS             7187..7504
                     /locus_tag="ON516_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345067.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7497..7679
                     /locus_tag="ON516_001578"
     CDS             7497..7679
                     /locus_tag="ON516_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345068.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7711..7800
                     /locus_tag="ON516_001579"
                     /pseudo
     CDS             7711..7800
                     /locus_tag="ON516_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7875..8342
                     /locus_tag="ON516_001580"
     CDS             7875..8342
                     /locus_tag="ON516_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCR3345069.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            8870..8942
                     /locus_tag="ON516_001581"
     tRNA            8870..8942
                     /locus_tag="ON516_001581"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8903..8905,aa:Lys,seq:ttt)
     gene            9328..9588
                     /locus_tag="ON516_001582"
     CDS             9328..9588
                     /locus_tag="ON516_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345070.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            9631..9804
                     /locus_tag="ON516_001583"
     CDS             9631..9804
                     /locus_tag="ON516_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345071.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            10193..11026
                     /locus_tag="ON516_001584"
     CDS             10193..11026
                     /locus_tag="ON516_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HCR3345072.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11019..12434
                     /locus_tag="ON516_001585"
     CDS             11019..12434
                     /locus_tag="ON516_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR3345073.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12446..13975
                     /locus_tag="ON516_001586"
     CDS             12446..13975
                     /locus_tag="ON516_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3345074.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            13893..14945
                     /locus_tag="ON516_001587"
     CDS             13893..14945
                     /locus_tag="ON516_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR3345075.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14947..15264
                     /locus_tag="ON516_001588"
     CDS             14947..15264
                     /locus_tag="ON516_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345076.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15391..16014
                     /locus_tag="ON516_001589"
     CDS             15391..16014
                     /locus_tag="ON516_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCR3345077.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16029..16919
                     /locus_tag="ON516_001590"
     CDS             16029..16919
                     /locus_tag="ON516_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HCR3345078.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16942..17157
                     /locus_tag="ON516_001591"
     CDS             16942..17157
                     /locus_tag="ON516_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345079.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17169..17510
                     /locus_tag="ON516_001592"
     CDS             17169..17510
                     /locus_tag="ON516_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345080.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17510..17881
                     /locus_tag="ON516_001593"
     CDS             17510..17881
                     /locus_tag="ON516_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345081.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17874..18269
                     /locus_tag="ON516_001594"
     CDS             17874..18269
                     /locus_tag="ON516_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR3345082.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18271..18648
                     /locus_tag="ON516_001595"
     CDS             18271..18648
                     /locus_tag="ON516_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345083.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18649..19257
                     /locus_tag="ON516_001596"
     CDS             18649..19257
                     /locus_tag="ON516_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345084.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19257..19598
                     /locus_tag="ON516_001597"
     CDS             19257..19598
                     /locus_tag="ON516_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345085.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19840..>21637
                     /locus_tag="ON516_001598"
     CDS             19840..>21637
                     /locus_tag="ON516_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR3345086.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGVGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESA"
BASE COUNT         7741 a         3234 c         4460 g         6202 t
ORIGIN      
        1 gcaccgtcta aaataaaatc cttttcttta atctttaata aagaaagttt gttttttatt
       61 tcggatgaag aagtttcatc tatattcgga ataattactg atttatagat agtgaccaga
      121 gatgtgtcat atttttgatt aatttcatcg tctaaccgct ggctttcagc attaagaata
      181 tcaattttgt tttctagcgt tgacaagttt ttattcaatt tacttatgtt tttctctttt
      241 tcagatataa acgtttccct agcatttaat tcttgagaat attcctttac cttactctct
      301 aaggcagaag ttttatcttt taatttttta gcttcagata atttgctact acccataatt
      361 attaaaacta ttccaactaa aaacaatagt actcccattc aaaacgcctc gcttctttta
      421 taaaataatt gtcaaaatca tataaaccct taccgaagtt gacggctccg gcatgggaat
      481 ttttttaaaa tacaactttt ccgataattc taatctgttc tggcgataca attctatctt
      541 cgtataagtc gtttatagaa cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct
      601 ctttacatgt aactccgtca ccacctaatt ccacaatagc aatttcccca tcttctacgt
      661 ccggttgttc tttaacgaat acgaattgtc cattcctgat aacaggttcc attgaatcgc
      721 ctataatagg tactgcgaag tcagcagcac ttggaactct ctcaaattct tcctcttcga
      781 caaccgtatc accgtaagta agttctgttg gattggcagc cgaagccgaa ttgtgaacag
      841 ttgggatttt ttctttttta ggaaaatcta ggacattttc ttgtttttgt tcttctaatc
      901 gattacttgc aaattcatag gtagcaactt tattttcgtc gttaagctga ttatatatag
      961 gaaggatatt atatatctga ttagatatag ataaatcaat attcatcaaa tcatctatat
     1021 caacattgaa tatcttagct atctcattta aggtttttaa ttttggtgta tattttcctt
     1081 tttcccattc gcttattgag gatgaacttt ttcttcctag tttttcggct agttcttgtt
     1141 gatcaatatt atacttttct ctaagaaact ttagattttc agcgaacata gtaacacctc
     1201 cttgagttaa ttgtaacacc gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt
     1261 agtgttttta aagaagtatt tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg
     1321 cacttgactt cggaaaaacc gaagtatata atttagtcag aaagttaaag agaggaggcg
     1381 aataaaatat ggataccgcg aagaaagaaa aatatacatt gagacaatta agagctttaa
     1441 agggttattc aaaagaagaa ctgtcaagga aaagcaaagt gacatcaaga acaatattta
     1501 tttacgaaaa cgatgtagat aaaatgagga acggaaagta cgccactcta gaaaaaattg
     1561 ctaaggcatt gggtgttaga gtttccgata tttttttaga ccctgattcg gaaaaaccga
     1621 aatcaataac aggtgaaaca aaacaagtca gctaggaggt gtgaatagtg gaagagaaga
     1681 atataaaagt accgactctt gaagaatata caaaaattgt tgtcgaaaca gacgagcaga
     1741 acccgaaaac aatcgcagtt attacggcga atgatattga atcatgcgaa ggttttcgag
     1801 ttcgaatgac ccctagatat gattaatgtt ctttatcacg tggaggtata ggatcgtttc
     1861 caaaactatc ttttgattgt atttgtccat tctttttttg gacaataaac tctgattgct
     1921 gttttttaga aatcttttta ccatactcaa cagcatctga tttattatca aatagttttg
     1981 tatcttcgga attaccttca ccacgtacag cccatttgcc atttctagga gatacccatt
     2041 gatttttacc cataacaatt cacctcgctt tcaagttaat tctattaaga ggtgaaaaga
     2101 aaacaaactg atggaggtga gattgtgagt aacaagccaa aaatcacttt gaatggcaca
     2161 cctaatcttc aaagattagc agatgaaatg gctagaatac gttctagaca ttggggagtt
     2221 actgtggtgg caaccataat tgaacaaaaa gaaaagccca cacagccggc aagcaagtag
     2281 tgggcaccat agaacaataa tattatatca cacactcgcc acttccgaaa agaggagttg
     2341 ctgctctcct ttcaagtttg ccaaagtcca cgtttcttca ttttggcaac tcctcttctt
     2401 ggaggtggtg acggcgagag acgaaggagg gatgacatga aggaagaatc aaaaataggg
     2461 attttgaatg agaacaatta cacaagatta attattgaaa cagaagaagg ggaaaaagta
     2521 gcagaagtta cattgaccga tgcaactcct gctgacggtt atcgtattcg attaactcct
     2581 aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt gactatcacg gctttgaatt
     2641 tttccatctc tgccatgaat aaatagctca gatccttgat ttttagaaat ctctctagcg
     2701 atttttactg cttctgattt tgtattagta tgtgcagtcg ctttagaatt tccagcacct
     2761 ttgacattcc aaccaccatt ggaattaggt acaacatgtt gattttttcc catagttttc
     2821 acctccattt ctatagaaat attttatcaa atagaaatgg agaaattcaa taaacgaaag
     2881 gagggatcaa atgacattac aagagcatca acacttgatg cgaaaactaa atcaggaata
     2941 ccacgaggaa atgaaaccta gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg
     3001 gttagaaata aaaaagtgac tcagccgtcc aaagcaatga gtcacaaacc aaaaattata
     3061 cctaaggaga ttttatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg
     3121 tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa
     3181 aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca
     3241 tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta
     3301 cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa
     3361 caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat
     3421 gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt
     3481 tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc
     3541 agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga
     3601 ttatgctaaa ccaccatatg aagcgccaat tgatgaggag gatgaagatg agtaaatcta
     3661 ccttagaaat gagccatcaa gaatggctcg aagaccgcaa aagaggtatt ggtggctcag
     3721 atgttgcaac cgtacttgga ttaaacaaat acaaatctcc ttatcaattg tggcttgaga
     3781 aaacgggtca aattgaattg aaagatttag aaagcgaacc agcttattgg ggtaatgttt
     3841 tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa
     3901 accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag
     3961 tgggagaaaa tgccattctg gaatgtaaaa cagccaatca atttctcgga aaagagtggg
     4021 aaggtgaaga ggtaccgctt agctatctct gtcaagttca gcattacatg aacgttttaa
     4081 acaaagacta ttgttatatc gctgttttga tcggtggaca aaaattcatt tggaagcgga
     4141 ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa
     4201 cgaacgttct tggaggtatc gagcctgtaa ttgacggaag tggagcgact gctgacttct
     4261 taaaagaaaa atatgcagat gtagaagaaa atcaaacagc tctaccatca cgttttgatg
     4321 aacttatcga gcaaaaaaga gaactcaagc ggacgaaaaa agaaattgaa tcatctattc
     4381 gtcaagtaga caacgagatc atcagtgagc taggtaaacg tgaagccagt atcggtatca
     4441 cacaaagaaa cattatcagc tggaaacttg ttcgtacaag acggatgaac acgaagaaac
     4501 tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa
     4561 gaaggctaac cgaaaaggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa
     4621 ttggcagaaa agccacagaa acaagttgca ccaggacagt tagggcttaa agctctaatg
     4681 aatacaccaa caatgagaaa gaaatttgaa gaagtacttc atgacaatgc taatgctttt
     4741 atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg
     4801 tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta
     4861 ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag
     4921 aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa
     4981 tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg
     5041 acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt
     5101 ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc
     5161 gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg
     5221 gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg
     5281 ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt
     5341 caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa
     5401 gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct
     5461 aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata
     5521 cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc
     5581 aatccttata tgtataaatt atggagcttg tgtttgatga aagcgagtca tgagaatcgc
     5641 aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc
     5701 gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt
     5761 tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact
     5821 acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa
     5881 atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat
     5941 tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac
     6001 gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat
     6061 caggaaatca aaaagccgaa tctgcaaagt tgggcagata acattcggaa aatgatcgag
     6121 atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg
     6181 ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca
     6241 gcgcaagcaa atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt
     6301 agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca
     6361 tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc
     6421 ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta
     6481 tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca
     6541 acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc
     6601 aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag
     6661 cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg
     6721 ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag
     6781 tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg
     6841 atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga
     6901 ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa
     6961 aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac
     7021 gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt
     7081 acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt
     7141 ctcaaacagc agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa
     7201 acaagaaaaa agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga
     7261 cgaattatct caacaagaga gtcgaatggt tcggaagata agtaagctaa tcggtgcaga
     7321 tacgatggat cgatccaagc cattacccaa actgaatttt gaatcattta cgtttgagaa
     7381 atatagccat ctgagagagg taggctatag tgtaaatgct attcgaaaag cacttggggt
     7441 aggaagtagt gtatgggcaa aatggcggaa agaaaatgta aatgaggtaa atcaaaatga
     7501 ttaagctaat aggcaaagag acgctcaagg tttactatgt agcggaaaca aaggcagaag
     7561 tcagccgttg gctattgaaa atgtttaata agactagagc caatgcttca gtaagtcaga
     7621 aagcaagtgt atcagaaaca ggcatgcctg aagcaatgtg gatttcaagg aggaactgag
     7681 atggcaatga taccgaagtt tagagcgtgg gatagagaaa aagacagaat gaaggcagta
     7741 gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg tacaaaataa
     7801 aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta tatcataatt
     7861 gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca agcgcgaaag
     7921 atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca tatctatcag
     7981 ctagatgtag agtagggcaa cctcgagaac caaaagtgac agcatcttat tccttggttc
     8041 caccttctac agctgatcat atatttgaag cagaaagaat gctgatcgat aaagaagaag
     8101 cacaggaaga atttgagtat ctgcataaat tgtttgttcg aggctattca gcgatacagc
     8161 atccgcataa gcctgatgtg actgaaaggc gcaagaagat attctatgat cgttatatca
     8221 atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa tcggttaaag
     8281 tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt gctttcaagt
     8341 agccagtttt tacacttttt ataccctttt attaccaatt tggtttccat tttatacctt
     8401 ttttatacca atcacttacc tattcaacgt tgtattatga tagtgtcgaa agattaggaa
     8461 acaggatcga caaaataaaa tgtaagggag gaaatctccc tcatcgttta attaagcttc
     8521 gataggcagc agcggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt
     8581 tcttattatc tatcatccgt tgctgtctat tgttattatg tcactgtggc ggaaagggta
     8641 gacgcttaaa aataaggtca atacgtcgag ggatagcctt aacgttttat gatttgacca
     8701 tgcaaggttc gattccttgc cagcgactta gacgaaaggt agcttaaata ttaaccacat
     8761 caacatcgtc agcgagttga taaaggtaac tagcagactc taggatttaa gccagtctta
     8821 ccgattagtc tgttatagta gcaagcttgc tagaggtagc tcctctggtt gacgtgcagc
     8881 tcagttggta gagcgcctga cttttaatca ggaggtcgcc ggttcgaatc cggtcacgtt
     8941 aataaagaac ctacggaaac aatcaatctt atcggatgcc gattggttgg ctgaattaag
     9001 cagtgtgtct gtgaatcagt taataggcat aaaatactag cacagacgtg tgccactctc
     9061 aggtgtaggt taggagagaa acattagcaa ccgagggttg gaaatgggcg ctcaaagtac
     9121 acgagcaagg cgaggtcgat agtaatcgat ggaatcggtg taggttgcta ttacataatt
     9181 gattgggtta gattgagttt tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac
     9241 aggatcggaa acgtccctgc ctgtgcatta catattagat cactcgttga gtggtctttt
     9301 tattttagaa aagagaggat tttgaaaatg aatcatgaga agtttatcga aaaatgcaag
     9361 gctattgtac gtgaaagaat tgagaatgag attgctgacc taagtggagc agtacctgag
     9421 tttagtgttt tcatagtctg gtcatgcaaa acactacaga atagcaaagc attagtcagc
     9481 gctagcttaa aaggagcacc gtattttgaa attacgttga acggggacaa aggtgaaatc
     9541 tatgtagata cttatctcaa aaaatcaaat gaatgtatca aagtctagca ggtgctaggc
     9601 tttttcttta cataaaggag gaatgatctc atgaaacaat ataccgctaa agatttcgag
     9661 gaaatgaagc gattaaagaa ggactatgaa gaagttgata tggagctaac tgttggagtc
     9721 attcaacgaa gactgcgggt cggattagag acagcaaagg ctatttacaa tgatctaaat
     9781 gctattgaag agaagaatgg ctaatgagga actactggta tatatcgcta actaatgaat
     9841 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga
     9901 agtactctat tgtcgaaatg gggagagaag cagaaccatg tgagattgat gcgtgcaagc
     9961 tcgtttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga
    10021 atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt
    10081 agacaaagtt tccgatttaa agatattaat tgaagcagat ctaatgttga aagatattga
    10141 aaattagaaa acaaaactca acctaagaag attgcgaggt ggtgtgtatt gaatggcaag
    10201 acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa
    10261 gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa
    10321 atcgcaagac aaatggagcg ctgaattgaa aagtaacgtt accaatggca aaagtaacgt
    10381 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag
    10441 ccgagcttct ccgccagtgg gtaataaaaa cgctttgaaa acaggcgagt atgaaaccat
    10501 attttttgag acactaagtg atgaagagaa ggacatctat tctagtctga atgataatcc
    10561 ttcttttgtt ttgtctgaag aaatacgtct acttaagata agacaatttc gtatgatgaa
    10621 gagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
    10681 aagaaagatt aaaaatccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
    10741 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
    10801 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
    10861 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
    10921 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
    10981 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
    11041 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
    11101 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
    11161 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
    11221 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
    11281 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
    11341 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
    11401 ggtttgctac ggctagaaca gcgactaaac cagaaaatat gcaaggtttt cacgaggacc
    11461 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
    11521 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaacccc aataatattg
    11581 aaggtgtttt ttacgattcc cacaattcag accgtgataa atacagagtt cataaagtat
    11641 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
    11701 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttcccaaa ggtgcgttgg
    11761 attcatttat cagtcttgaa acggttgaat tggctacaga aaaacaaatt agtgattctt
    11821 tagtcaataa aacaacggtt gctcatattg gtgttgacgt agctcgatat ggtgatgatt
    11881 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
    11941 gtagcaccat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
    12001 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
    12061 ccgaccgcct agaagaactt atagaagaca aacattatcc ttttgaggtg tttggagtga
    12121 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
    12181 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
    12241 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
    12301 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
    12361 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
    12421 ttattcaatt ttaggaggtg agcttttgat tagtacagta ttatctttag agagatataa
    12481 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
    12541 tatagaagat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
    12601 cgaaaagaat gaaatagata atagaccaaa gcctaacact gatattgtaa aagttaataa
    12661 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
    12721 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
    12781 aaaagataaa ttcttaaaaa atgatataga acttccgaca gttccagaag cttttaacaa
    12841 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
    12901 tcaaggagct tgtggagtag ctttccgatt gttagagcct gtggaagaag atgatggttg
    12961 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
    13021 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
    13081 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
    13141 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
    13201 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
    13261 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
    13321 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
    13381 tatcaactta aaagacccta ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga
    13441 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
    13501 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
    13561 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
    13621 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
    13681 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
    13741 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
    13801 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
    13861 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
    13921 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
    13981 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
    14041 acttacacaa tatcacttat ccagaaatga tggagtttta tagtacaagt aatcagaaaa
    14101 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
    14161 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
    14221 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
    14281 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
    14341 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
    14401 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
    14461 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
    14521 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
    14581 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
    14641 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
    14701 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
    14761 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
    14821 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
    14881 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
    14941 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
    15001 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
    15061 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
    15121 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
    15181 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
    15241 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
    15301 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
    15361 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
    15421 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
    15481 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
    15541 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
    15601 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
    15661 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
    15721 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
    15781 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
    15841 tttacaagtg tcttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
    15901 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
    15961 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
    16021 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
    16081 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttatttggt gctggaaaaa
    16141 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
    16201 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
    16261 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
    16321 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
    16381 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
    16441 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
    16501 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
    16561 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
    16621 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
    16681 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
    16741 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
    16801 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
    16861 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
    16921 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
    16981 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
    17041 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
    17101 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
    17161 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
    17221 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
    17281 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
    17341 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
    17401 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
    17461 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
    17521 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
    17581 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
    17641 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
    17701 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
    17761 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
    17821 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
    17881 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
    17941 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
    18001 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
    18061 ctagaaaact atcaagcggt cgaattgttg gagcagttat aaacaatgaa cattatttta
    18121 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
    18181 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
    18241 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
    18301 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
    18361 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
    18421 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa acctaataga
    18481 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
    18541 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
    18601 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
    18661 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
    18721 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
    18781 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
    18841 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
    18901 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
    18961 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
    19021 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
    19081 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
    19141 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
    19201 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
    19261 caaaatatca aacaacgatt aaattaacga aaaaagatga agaaggtaaa tatgagcaag
    19321 tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta
    19381 tggaagaaat gcaaacagcg actgataaac aatcagttaa aaaagcttta agtcgtgctt
    19441 attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag
    19501 gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag
    19561 gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc
    19621 atttaagtat tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg
    19681 ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt
    19741 tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta
    19801 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
    19861 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
    19921 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
    19981 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
    20041 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
    20101 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
    20161 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
    20221 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
    20281 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
    20341 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    20401 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    20461 ttgtctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    20521 aaaggtgtcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    20581 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    20641 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    20701 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    20761 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    20821 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    20881 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    20941 tcattaaaga ttatgggggc aatagctgct gctaattcat ttgttttaat tattggtgca
    21001 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gtttagaaac
    21061 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    21121 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
    21181 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    21241 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    21301 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    21361 aaagattcag ccagagaaaa atggaatgaa attaaaggtt ctattatgtc ggttgctggt
    21421 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    21481 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    21541 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    21601 atgattgctg tttggaataa tattaaagaa agtgcca
//
LOCUS       DAJSNV010000046        21285 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSNV010000046 DAJSNV010000000
VERSION     DAJSNV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.047"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            269..475
                     /locus_tag="ON516_001599"
     CDS             269..475
                     /locus_tag="ON516_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345087.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            671..838
                     /locus_tag="ON516_001600"
     CDS             671..838
                     /locus_tag="ON516_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3345088.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            864..1208
                     /locus_tag="ON516_001601"
     CDS             864..1208
                     /locus_tag="ON516_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3345089.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1213..1494
                     /locus_tag="ON516_001602"
     CDS             1213..1494
                     /locus_tag="ON516_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345090.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1597..1911
                     /locus_tag="ON516_001603"
     CDS             1597..1911
                     /locus_tag="ON516_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3345091.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1889..3583
                     /locus_tag="ON516_001604"
     CDS             1889..3583
                     /locus_tag="ON516_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3345092.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3603..4781
                     /locus_tag="ON516_001605"
     CDS             3603..4781
                     /locus_tag="ON516_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3345093.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4744..5430
                     /locus_tag="ON516_001606"
     CDS             4744..5430
                     /locus_tag="ON516_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3345094.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5430..6590
                     /locus_tag="ON516_001607"
     CDS             5430..6590
                     /locus_tag="ON516_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3345095.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6600..7475
                     /locus_tag="ON516_001608"
     CDS             6600..7475
                     /locus_tag="ON516_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345096.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7472..7783
                     /locus_tag="ON516_001609"
     CDS             7472..7783
                     /locus_tag="ON516_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345097.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7773..8126
                     /locus_tag="ON516_001610"
     CDS             7773..8126
                     /locus_tag="ON516_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3345098.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8116..8517
                     /locus_tag="ON516_001611"
     CDS             8116..8517
                     /locus_tag="ON516_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345099.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8510..8914
                     /locus_tag="ON516_001612"
     CDS             8510..8914
                     /locus_tag="ON516_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345100.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8926..9531
                     /locus_tag="ON516_001613"
     CDS             8926..9531
                     /locus_tag="ON516_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345101.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9551..9913
                     /locus_tag="ON516_001614"
     CDS             9551..9913
                     /locus_tag="ON516_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345102.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9916..10098
                     /locus_tag="ON516_001615"
     CDS             9916..10098
                     /locus_tag="ON516_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345103.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10115..13546
                     /locus_tag="ON516_001616"
     CDS             10115..13546
                     /locus_tag="ON516_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3345104.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13597..14334
                     /locus_tag="ON516_001617"
     CDS             13597..14334
                     /locus_tag="ON516_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345105.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14344..16635
                     /locus_tag="ON516_001618"
     CDS             14344..16635
                     /locus_tag="ON516_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345106.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16659..18785
                     /locus_tag="ON516_001619"
     CDS             16659..18785
                     /locus_tag="ON516_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345107.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18802..18951
                     /locus_tag="ON516_001620"
     CDS             18802..18951
                     /locus_tag="ON516_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345108.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            18948..19394
                     /locus_tag="ON516_001621"
     CDS             18948..19394
                     /locus_tag="ON516_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345109.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19396..19533
                     /locus_tag="ON516_001622"
     CDS             19396..19533
                     /locus_tag="ON516_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3345110.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19571..19864
                     /locus_tag="ON516_001623"
     CDS             19571..19864
                     /locus_tag="ON516_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345111.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19861..20085
                     /locus_tag="ON516_001624"
     CDS             19861..20085
                     /locus_tag="ON516_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3345112.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20082..21107
                     /locus_tag="ON516_001625"
     CDS             20082..21107
                     /locus_tag="ON516_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3345113.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         7503 a         3220 c         4463 g         6099 t
ORIGIN      
        1 ttaaaaataa ttgcaagaaa agaaatttat agtggtttat accaaattat caaagcaatg
       61 ttattttgtt gctgtctatt gttttttaat tattcacacg ataactaaag gtgggtgaag
      121 agaaatgatt ccattaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
      181 atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat agtgtcactg
      241 tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca accatgcaag
      301 gttcgattcc ttgccagcga cattaaatgc ctatgacggt tacgactacc gaaaaaagat
      361 cgttaagaag ctatacggtg ctacgtacgg caatgtagta agtgtgctat ctgtacacca
      421 ccaagcttcg gtcactgtgg cggaagtaga agacgcagcg gtaaatggcg agtagcctcg
      481 tgagagcctg gtaagttctc gtgagtggtg caatcccact ccagcgactt taagcaaccg
      541 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
      601 caagttacta ttacatatta gatcactctt tgagtggtct ttttattttt gcacaaagga
      661 ggaaacaaca atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgttca
      721 taaaggtaat ggcgaaaaag tatatgagta tagaagacca ataaagagcg atacatataa
      781 acgaaaggaa aacaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
      841 aagattgcga aaggagacgg aacatgaccg aggaattcta tagatggcta ttacagttga
      901 caagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga
      961 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
     1021 gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
     1081 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
     1141 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
     1201 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
     1261 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
     1321 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
     1381 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
     1441 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
     1501 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
     1561 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
     1621 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
     1681 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
     1741 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
     1801 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
     1861 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
     1921 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
     1981 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
     2041 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
     2101 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
     2161 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
     2221 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
     2281 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
     2341 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
     2401 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
     2461 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
     2521 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
     2581 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
     2641 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
     2701 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
     2761 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
     2821 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
     2881 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
     2941 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
     3001 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
     3061 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
     3121 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
     3181 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
     3241 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
     3301 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
     3361 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
     3421 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
     3481 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
     3541 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
     3601 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
     3661 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
     3721 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
     3781 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
     3841 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
     3901 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
     3961 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
     4021 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
     4081 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
     4141 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
     4201 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
     4261 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
     4321 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
     4381 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
     4441 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
     4501 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
     4561 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
     4621 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
     4681 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
     4741 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
     4801 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
     4861 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
     4921 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
     4981 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
     5041 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
     5101 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
     5161 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
     5221 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
     5281 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
     5341 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
     5401 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
     5461 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
     5521 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
     5581 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
     5641 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
     5701 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
     5761 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
     5821 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
     5881 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
     5941 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
     6001 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
     6061 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
     6121 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
     6181 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
     6241 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
     6301 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
     6361 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
     6421 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
     6481 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
     6541 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
     6601 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
     6661 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
     6721 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
     6781 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
     6841 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
     6901 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
     6961 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
     7021 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
     7081 caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa aaggtttatc
     7141 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga
     7201 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
     7261 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
     7321 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
     7381 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
     7441 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
     7501 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
     7561 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
     7621 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
     7681 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
     7741 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
     7801 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
     7861 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
     7921 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
     7981 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
     8041 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
     8101 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
     8161 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
     8221 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
     8281 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
     8341 gaaaatagag aagatttaac aggagattca ctagatatta tttcattgat tcaaaatcgt
     8401 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
     8461 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
     8521 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
     8581 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
     8641 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
     8701 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
     8761 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
     8821 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
     8881 tctgattcgt caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca
     8941 acagtaacaa cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca
     9001 acaaatgcaa tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga
     9061 attgtaaaaa aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg
     9121 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa
     9181 gatgaacgta ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc
     9241 tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta
     9301 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
     9361 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
     9421 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
     9481 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
     9541 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
     9601 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
     9661 cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc
     9721 aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt cattcgatga
     9781 atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat
     9841 gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa
     9901 gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
     9961 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    10021 attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac
    10081 caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga
    10141 agtaacattc atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga
    10201 attaactaaa aatagagcag aattaaaact agaacaagct caattacaac agactggttc
    10261 tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag
    10321 tcaaaaagtt gaagtaacta gccaacgttt agccaatgcc aaaaaatatt atggagaaaa
    10381 ttccaccgaa gttcagaaac ttgagagaga actgattaac caacaaacag cgcaacaacg
    10441 tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca
    10501 gacgtacgag tctacaatgc aacagttgga tagtgaacaa aaaaatgttc aagctagtgc
    10561 ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga
    10621 atccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcagagaa
    10681 aacgatagat atcctgagac gacagttaga agctacacag tctgagtttg gcgctacatc
    10741 cacagaagca atgcagatgg aggcgaagct taatgatgct gaacgtgaat ttgaagagtt
    10801 aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga
    10861 tatgaataat ctaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga
    10921 attagggaaa caagcagtgg actctgctaa tagtgtaggt agttcccaga gtaaaataca
    10981 agctaatttt ggtttgacta aacaagaggc tgaagaatta acgaatgtag ccagagacat
    11041 ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa
    11101 gcgtaattta ggcgatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt
    11161 cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat
    11221 ggtcaatttc ggcttgagcg ctcaagatgc aatggattta atggtttcgg gtactcaaaa
    11281 cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag
    11341 tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg
    11401 tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga
    11461 tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga
    11521 gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag
    11581 caatatggat ggtcaatacg accaattaaa taaagcttct acaatttggt ctgcacttgg
    11641 cgaagataac gcgatgaaag ttgtccaatc tttgactgat gttaaccata catttgatga
    11701 tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttac aagaattaaa
    11761 cggaaaaata gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc
    11821 actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca
    11881 accagtacag gatttcattg ttgctatagg tggtcttaca gcagcatttg gcatactgtt
    11941 accggcaata ctagccgtgt catatttatt tggtccgatg atgcttataa ttggaggaat
    12001 tatagctgtt atagcaggcg ttattgtggc aattaagaac tggggtgcaa ttactgactg
    12061 gtttagtggt ttatggaaaa aatttactga ttggttgggt gatacttggg aaagtataaa
    12121 agacggagcc tcatcagttt gggatggggt taaagaaacc tggtctggat ttgtagattg
    12181 ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt
    12241 tgaaggagct tccaacatct ggcaaggagt ccaagaaact tggcaaacat tcgttgattg
    12301 ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt
    12361 aggaattgtt caaataccat ggacattaat aacgtcattg attcaagctg gtattaatat
    12421 tatcgtgggt atttttgatg tagctggaca gttattgggc gcagcttggc aagctgtttg
    12481 gacacctatt tctgatttcc ttaaaaatac ttgggatact atgacacaat ggataagtat
    12541 cgcttggaat gggattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
    12601 gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc
    12661 aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc
    12721 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
    12781 ttcaatcagc tctacagcat caaatatctg gaatagcgtg actagtagta taagcaatgc
    12841 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggattag
    12901 cggaatttgg aacggtatca aaaatactgc tttgaatctt tggaatggaa ttacaagcac
    12961 tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac
    13021 aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca tatgggaagg
    13081 catcaaaaag actgttagcg ctccaattga ttggattaga gacaaaatca gtggcatttt
    13141 tgataatttg aatatttcta taccacatat tccgttaccg gaatttatca tggaaggcag
    13201 ctttaacccg ctaaaaggtc aaatccccca tttgcgtgta aaatggcatg ctaaaggagg
    13261 tatctttact aaaccaactt tactaggtgg aatgaacggt gtcggtgaag caggacctga
    13321 agcagtttta cctttgaaaa gatctgtttt gcaagaaatt ggtgatcgta tcttgagtag
    13381 cacatcagtt tcatctaggg cacaaacgat tcaacctgtg aataactacg aattcaattt
    13441 cacaattgat ggtaacgcag atgaggttac tatgaagcaa acaactcaac aaatcattga
    13501 tagcattaca aaaattcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt
    13561 ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga ctgattgtat acattctata
    13621 atcgatggat ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct
    13681 tctccaaaaa gacagagagt tgaaacttct gttttaggta ggttaggtgg cttagtacaa
    13741 gattactcgt ttgaagatat gtcgtttaca ttgcactata actatttaga ggatgtggaa
    13801 gaccatcaag cgttcaagca atcgttttat atcatgcgtc attggttaaa ctatgcaaag
    13861 aaattagaat tctctgacga tcccaacgtc tattacgtta tccagactat cgatattggg
    13921 gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatattac tgcgaaacca
    13981 ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg
    14041 ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc agctacttca
    14101 tgccagttca tcttaaatga ttatgtgttt agtttcgaag gcttagtagc aggaactgat
    14161 gtagtcattg atagtgattt gatgctttgc tacgaagagc aatcggacgg agatatttta
    14221 gatcggtcca acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat
    14281 tttaattgca ctggtttgag caaaatacaa atttatcgta acgggttaag gtaggtgaaa
    14341 tagatgatcg ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa
    14401 catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc tactgttagc
    14461 aataaactaa acggagccga gatatttcag ggtacttatt caataaacgg aactaatgca
    14521 gatttgattg tagacggacg tattattcag tgttatgtag atgaaaatcg agcaaaacag
    14581 cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga agtaaaagct
    14641 gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag cggaacagaa
    14701 aagatcactg ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc agctatccct
    14761 tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaggcg
    14821 aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggtgag
    14881 tttctaaaag ataataacac attacgtcat gaaaaaagtc taggtacgga tcataaaatc
    14941 aaagcgattt atactaaaaa cttaactggt cttgacttag agattgatgc tcaaagtgtt
    15001 ttagttggag tttatccatt cattagcagc tcttcagaag gagaagacga gatcactcta
    15061 ccagaagaag ttattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt
    15121 gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg
    15181 ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca
    15241 ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac
    15301 ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat
    15361 acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac
    15421 ttcttagaaa acacagagaa taatgtcagc aatttgatta atgatgccat tgatcaattg
    15481 aaaaacggtg gcgaaatcag tgatttactc aatgatattg tagatcatca aactgatatg
    15541 attactggtc aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt
    15601 ttgattatgg acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct
    15661 ggtattggtt tttctaaaac tggcattaat ggaacatatg aaacggcatg gacgttagat
    15721 ggcggattca atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt
    15781 actacattaa ttagtgatgg tgctgattat agaacaagta ttgctaatgg caaaatgact
    15841 tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt
    15901 gctgatgccg gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat
    15961 ccacagggta acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct
    16021 tttggtgcgc ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt
    16081 tctttaagca ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact
    16141 atgaaattta ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg ttcatgggaa
    16201 gatgggaaac ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt
    16261 agaggtacta aaaactcaac tgtaccaaca gaacattatg gacaacgact attgaacgct
    16321 tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg tgacaatggt
    16381 aaagttcgtg ttgatattga ccccatgttt gctgaaacag taaatctaag tcggtatatg
    16441 acacatgtga cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat
    16501 ttcatcattg aaactagtaa gccaaacgta ttagttagat ggaatttagt ggcacaccgt
    16561 ctagggtatg aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac
    16621 caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt
    16681 tggctttaga tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcaaggtg
    16741 ataaagggat tacggtgacg gttaaaccgt ttctaaatgg cttgcagatg gatacgagtg
    16801 gcggaacatt tactttaaaa ggaacaacac catctaaccg ttacgtagat aatgttgcaa
    16861 ctagtgtaac tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag
    16921 gatattataa acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag
    16981 atatcatttt tttctcacta ggagtgtctg acatttcgca aggccaagcc gatgaatatg
    17041 tttcgcaatt agaagagttg attcgaaagt acaacgaaac ttttgatgct tttatggctg
    17101 aaatcaaagg tagagtggat agcttaaatc aacagattac tgatttaact ggtcaagcta
    17161 aaacgctaca agacaagtta gatgctctga aagaagaaat ttctaagtta ggtaacttac
    17221 aagtgatgta cagtaacagc atcgacttcg gggactatga ttattcggag aacccgaatt
    17281 taatgccata tattacagaa ccttgggttg gacctttgtt aggtaatgga catacagtta
    17341 aagatagtgt taaaagggtt ataactcata ctaaaactag aacagctaat tccggcgaca
    17401 tattatcgct tggcttaggt attccgtgta cagcggaagc taataatagg tatcttataa
    17461 ccactttacg accatctacg acatacacat taagcgttac tatgagtgtc ggttcagatt
    17521 ggactggaga gaccaatact ataggggtta ggcttagata tttaaatgaa caaggtggta
    17581 tagaactgcc cattaatgca ttaataccag caaatgtaga acgcgataaa atggtcactc
    17641 atacatttac tggtataaca aaagataatg taactagcat aacaaactgt tatgttgaaa
    17701 tattctcact taatagtgaa tataaaggaa cggttagtgt cagctatgac gttaagttaa
    17761 aagcacacta ccctaactta ctagatggcc cttactggtt aggtaaagtt cctttaggcg
    17821 agaatattgc tgaccctaca gttgtctttc cacacaaaac tagtgaatat atggtttatg
    17881 gtagacgcaa tacagaaaac tatatcgctg accaaacata taccatatca atgaaagcaa
    17941 ctaaactaac agttcaatcg tttgccgtgt atattgctgc tggccgcgtt aaagtaggag
    18001 acatgaagcc tactgagggg ttagctaata catgggagtt aacttttact gtaactaagc
    18061 aacatataga tagtggggta actaattatc tggaaatata ccaatacccg tcagctacaa
    18121 aaggagctgt tcaaattgag tggctcaaac tagaaaaagg caatactcga accccgaata
    18181 ttagtgagta taaatatcgt ggtactggta tgcgtgattc aaacaatcca aaagattatg
    18241 tttgggatct agcaccagaa tatgtcgaag ataatttggc cacagatatt aaaatttctg
    18301 aaattactgg taaagcaaac aattataccg atgggaaagt atcggagatt aattcgcagt
    18361 tgactgcttc aattaatgaa gtagacacca cagctaagga tgctcaaaca aaagcgaatg
    18421 ctaatgcgac tgctatagat gaattagaca ataagatcga tgaacgcatt aatgatacag
    18481 ctactaccac attaacagtt acaaacggga ataccggatc agcaaagctt tatcgtgaag
    18541 gaaaaacagt ttctatatat tttgtggctt taaacggaaa acgcagtggt ggaaatgatt
    18601 caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag caactggttg
    18661 gctcgataga ccgttctact ttgaacagtg ctcagttatc tattggtgca gatggagcca
    18721 ttaaatggcg aagaaactca agttatggat cggattatac ctttgcaatt acttacacga
    18781 tttagaaaag cgtgaatcga tatgaaggca gcatatagac caattgaacc ttacggattc
    18841 gagcaaatca ttgtgaatga tgaagaacat ttaccggaag aatgcacaga agtcgaacca
    18901 ccgattccaa attggaaacc gaaattcaat tactgggagg gaaataaatg aaaaacattt
    18961 ggaaatatgg acgtactggc ggagagtacg caggaaaagt attggacgac atgcttgtat
    19021 ccgttcctta cacagatcag ccaccgcttg aaggaattcg tgctgatggc gaaccgttaa
    19081 caatcgctga tcaaatgttt gatcctaaat tgaatcaatg gattatttta gcgaacgcac
    19141 tagatcacaa cgatttaaac aatctcaaag caatgtatga gtcgttagaa aatgagaacg
    19201 gcgatttaaa acagatcaat gccaaactca tgctaagcga tgtagcaatt aaacaggaaa
    19261 atactgcatt gaaagaaaaa gcggatagtt tagcacaaat caattcaaaa atgatgcttg
    19321 cttcgttaca aaatagcaaa gacatttcag aaattaaaga gcaactaaat ccagcttcaa
    19381 agggaggtga gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt
    19441 ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga aaaagcagat
    19501 aaaatcatta gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag
    19561 gagttgtcac atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat
    19621 cgtttctttg attttattag taataaaacc aattactgca tctttctcga agattactga
    19681 gactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa
    19741 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
    19801 tgcagaaaaa ttggtagaac acacgcaaca aattaaaact ttatttaggg agagaagaaa
    19861 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
    19921 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
    19981 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
    20041 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
    20101 ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg tttgcttata
    20161 ctattaatga cgagtataat ttagcgccga atcaaggaga ctccagatta gcaattccta
    20221 acaaaattat tttgcacgaa actggaatag atgcaccagc aagaaacgta gccgccaaca
    20281 tgaaaaataa ttataacgga agcaattctt atactacaga tgttattggt gacggtggga
    20341 ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg agctggtaac gctaatcctt
    20401 atgcgcctgt acagattgaa ttacagcgca catatgataa agcattgttt gaaaaaaact
    20461 atcgagctta tattgaatat acaagagata gtgcaaaaaa atatggaatt ccattgactc
    20521 ttgatcaagg aacttcttta tttacaaaag gaatcatttc tcatttgtgg gtgacaaatt
    20581 atgtttgggg gaaccacaca gatccatatg gttacttatc gcaaatggga gttagcaaag
    20641 aaaagcttgc ttatgattta gctcatggat ttaccgatga aaatccaaca acttctgaaa
    20701 acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg acagatggaa
    20761 caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg catgtggctg
    20821 gatggcacat tgctaactat aaatacgagt atattttcat tatggactac aatactggga
    20881 aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac caagcttata
    20941 atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt cctagtaaga
    21001 aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc
    21061 aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg
    21121 ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa aatagtgatg
    21181 ttatcgcata tcttcactat cacccataaa tagtcacact ccaagctatg cgataacagg
    21241 tttgttgcca cacattctac tggttgattg tttatggctt tatgt
//
LOCUS       DAJSNV010000047        20604 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSNV010000047 DAJSNV010000000
VERSION     DAJSNV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.048"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            352..1041
                     /locus_tag="ON516_001626"
     CDS             352..1041
                     /locus_tag="ON516_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345114.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ON516_001627"
     CDS             1028..2236
                     /locus_tag="ON516_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3345115.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ON516_001628"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ON516_001628"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3345116.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ON516_001629"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ON516_001629"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3345117.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ON516_001630"
     CDS             complement(6339..7034)
                     /locus_tag="ON516_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3345118.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ON516_001631"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ON516_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3345119.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ON516_001632"
     CDS             complement(8233..8424)
                     /locus_tag="ON516_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3345120.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ON516_001633"
     CDS             complement(8445..9335)
                     /locus_tag="ON516_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3345121.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ON516_001634"
     CDS             complement(9322..10089)
                     /locus_tag="ON516_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3345122.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ON516_001635"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ON516_001635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3345123.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ON516_001636"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ON516_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3345124.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ON516_001637"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ON516_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3345125.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ON516_001638"
     CDS             14660..15043
                     /locus_tag="ON516_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345126.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="ON516_001639"
     CDS             complement(15211..15969)
                     /locus_tag="ON516_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3345127.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ON516_001640"
     CDS             complement(15987..16805)
                     /locus_tag="ON516_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3345128.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ON516_001641"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ON516_001641"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3345129.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ON516_001642"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ON516_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3345130.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ON516_001643"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ON516_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3345131.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ON516_001644"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ON516_001644"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3345132.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5888 a         4210 c         3789 g         6717 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attt
//
LOCUS       DAJSNV010000048        20129 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSNV010000048 DAJSNV010000000
VERSION     DAJSNV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.049"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            79..1506
                     /locus_tag="ON516_001645"
     CDS             79..1506
                     /locus_tag="ON516_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345133.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1598..1963)
                     /locus_tag="ON516_001646"
     CDS             complement(1598..1963)
                     /locus_tag="ON516_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3345134.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1960..2325)
                     /locus_tag="ON516_001647"
     CDS             complement(1960..2325)
                     /locus_tag="ON516_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3345135.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2517..2765)
                     /locus_tag="ON516_001648"
     CDS             complement(2517..2765)
                     /locus_tag="ON516_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345136.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2764..2892
                     /locus_tag="ON516_001649"
     CDS             2764..2892
                     /locus_tag="ON516_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR3345137.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2949..4643
                     /locus_tag="ON516_001650"
     CDS             2949..4643
                     /locus_tag="ON516_001650"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR3345138.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4647..4766
                     /locus_tag="ON516_001651"
                     /pseudo
     CDS             4647..4766
                     /locus_tag="ON516_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4743..4916
                     /locus_tag="ON516_001652"
     CDS             4743..4916
                     /locus_tag="ON516_001652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345139.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5294..6427
                     /locus_tag="ON516_001653"
     CDS             5294..6427
                     /locus_tag="ON516_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR3345140.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6458..6778
                     /locus_tag="ON516_001654"
     CDS             6458..6778
                     /locus_tag="ON516_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR3345141.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6807..7271)
                     /locus_tag="ON516_001655"
     CDS             complement(6807..7271)
                     /locus_tag="ON516_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345142.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7403..8656)
                     /locus_tag="ON516_001656"
     CDS             complement(7403..8656)
                     /locus_tag="ON516_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345143.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8685..9002)
                     /locus_tag="ON516_001657"
     CDS             complement(8685..9002)
                     /locus_tag="ON516_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3345144.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9028..9348)
                     /locus_tag="ON516_001658"
     CDS             complement(9028..9348)
                     /locus_tag="ON516_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345145.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9812..11227)
                     /locus_tag="ON516_001659"
     CDS             complement(9812..11227)
                     /locus_tag="ON516_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3345146.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11244..12560)
                     /locus_tag="ON516_001660"
     CDS             complement(11244..12560)
                     /locus_tag="ON516_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345147.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12710..13408)
                     /locus_tag="ON516_001661"
     CDS             complement(12710..13408)
                     /locus_tag="ON516_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3345148.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13697..14653)
                     /locus_tag="ON516_001662"
     CDS             complement(13697..14653)
                     /locus_tag="ON516_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3345149.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14766..15599)
                     /gene="msrA"
                     /locus_tag="ON516_001663"
     CDS             complement(14766..15599)
                     /gene="msrA"
                     /locus_tag="ON516_001663"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3345150.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15774..17042)
                     /locus_tag="ON516_001664"
                     /pseudo
     CDS             complement(15774..17042)
                     /locus_tag="ON516_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17280..17516
                     /locus_tag="ON516_001665"
     CDS             17280..17516
                     /locus_tag="ON516_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345151.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            17523..19475
                     /locus_tag="ON516_001666"
     CDS             17523..19475
                     /locus_tag="ON516_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3345152.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(19547..20044)
                     /gene="dfrG"
                     /locus_tag="ON516_001667"
     CDS             complement(19547..20044)
                     /gene="dfrG"
                     /locus_tag="ON516_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3345153.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         6437 a         3916 c         3438 g         6338 t
ORIGIN      
        1 taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata
       61 caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg
      121 tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc
      181 ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa
      241 gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat
      301 atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt
      361 ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc
      421 tctgtcagca aagcttattc attaaaaaaa caagtattag cttactttaa tgactgcggg
      481 atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt
      541 aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa
      601 cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa
      661 aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct
      721 gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc
      781 gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt
      841 attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa
      901 gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt
      961 gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg
     1021 ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt
     1081 tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc
     1141 tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg
     1201 atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca
     1261 aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat
     1321 tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag
     1381 tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa
     1441 aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac
     1501 aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt
     1561 ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg acatctaggg
     1621 gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta
     1681 gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa
     1741 gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc
     1801 ttactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg
     1861 atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt
     1921 tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct
     1981 ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat
     2041 aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat
     2101 atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt
     2161 atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag
     2221 tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat
     2281 agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt
     2341 tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa
     2401 aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc
     2461 cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag
     2521 tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta
     2581 aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca
     2641 ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt
     2701 tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt
     2761 ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct
     2821 gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc
     2881 gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa gacattggag
     2941 gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag
     3001 cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat
     3061 tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga
     3121 ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta
     3181 attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc
     3241 cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca
     3301 aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata
     3361 ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac
     3421 tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact
     3481 tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc
     3541 gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca
     3601 atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag
     3661 aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac
     3721 cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag
     3781 aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag
     3841 attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag
     3901 cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat
     3961 tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt
     4021 tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat
     4081 ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg
     4141 tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt
     4201 tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa
     4261 aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata
     4321 ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc
     4381 cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta
     4441 aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct
     4501 ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc
     4561 ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg
     4621 ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa
     4681 taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc
     4741 agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag
     4801 taataagagc agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac
     4861 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc
     4921 gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa
     4981 taccgagaaa ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga
     5041 aaacaactat tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa
     5101 ctatttcaat gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata
     5161 cataagacta tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga
     5221 tctcctttaa aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa
     5281 aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa
     5341 cgtagatgat ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc
     5401 catttgcggc tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta
     5461 tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt
     5521 gaaaaaaggg caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg
     5581 cctgaatcac atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct
     5641 tttcggattt ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt
     5701 tccttatgca gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt
     5761 tttatttcta tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa
     5821 aaaaggcgat acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt
     5881 cgctgttatg gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga
     5941 taaagcggct aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg
     6001 aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat
     6061 ggacggatta gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac
     6121 aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac
     6181 tggtgtttat atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat
     6241 cgctgtcaag catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat
     6301 tgcagaagga ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc
     6361 agcacaagca tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa
     6421 accttgaatt cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac
     6481 ccatgagatc gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt
     6541 ctatttccaa aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt
     6601 ctatattttt ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt
     6661 ttatttggct ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat
     6721 ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga
     6781 atttttcaaa tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg
     6841 gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac
     6901 gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg
     6961 atttccgcta atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg
     7021 gcacaaagat ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct
     7081 cctccgacga tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta
     7141 ttagcgattc cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat
     7201 gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta
     7261 tatgtaacca atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc
     7321 tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac
     7381 ttgttctccg ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg
     7441 tagctacctt gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta
     7501 gtacaccacc tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc
     7561 atggaggcat aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac
     7621 taactaagac cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag
     7681 gtaacccaaa catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg
     7741 ttacaaccat atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta
     7801 aagttgcacc tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa
     7861 acggtgcgct atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga
     7921 ttgtttgcat caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg
     7981 gtgtgataaa tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt
     8041 cttggaccac tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg
     8101 cagcaactaa agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt
     8161 ttgcaactgc cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta
     8221 aacgtacaaa gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg
     8281 agctcatacc taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg
     8341 aaacaccgtc ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga
     8401 cagaaagaat ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac
     8461 caccaaaaat tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta
     8521 ctgctaatgc tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa
     8581 ctaaatgccg ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg
     8641 caacaaattt atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga
     8701 cgatacagat caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga
     8761 tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc
     8821 agcatctctg tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt
     8881 tcatctgcta aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt
     8941 tttgcatttc cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc
     9001 atttattgta tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag
     9061 tactttttca ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc
     9121 tttaggtgct agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag
     9181 taaaacgtct acatttttag attctagttg attatccgca tctgaagcag atacagcaaa
     9241 gatgtctgct tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact
     9301 tgtactcatt ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt
     9361 cctttctata cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa
     9421 aaagttgata tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag
     9481 cgcacactaa acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa
     9541 agttgtgaca caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat
     9601 ctgcggtaat aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct
     9661 tattacttga tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg
     9721 accctgggta ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta
     9781 aatacaatga acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca
     9841 attctctctt atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc
     9901 gttcatcgtt caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg
     9961 acacacagtc agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg
    10021 ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga
    10081 ttcctttctc atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca
    10141 gccaagggat attatgataa ttttctttta gatacatggc tatatcatac atgattttcg
    10201 gatagatttc ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat
    10261 aatatcgttc aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc
    10321 gtttaggata atattgattg attccaagga aatctactgt atgatgctga atcgtttcta
    10381 aatcctctgt tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt
    10441 ctgtgtagta tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca
    10501 tataagcagc tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta
    10561 aaatacagcc aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg
    10621 ttgctaaaac tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc
    10681 agcgaacagc atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc
    10741 agttttttac cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat
    10801 caactacttt tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat
    10861 aatgttccaa acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac
    10921 ttatatgctg agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag
    10981 cccaattgat agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct
    11041 gctggtattg ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa
    11101 aatgttcatt tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc
    11161 aaccttccgt ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa
    11221 ttttcatgat ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt
    11281 acaaatggca gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca
    11341 ccgatatttc ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc
    11401 attacaactg ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg
    11461 ataagaggcg ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca
    11521 aaaataactg gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt
    11581 ttcatatctt cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca
    11641 gacccgccca tactcatata catatcccaa aatggcatag taataatgtt cggtacttct
    11701 tttccagctt caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt
    11761 atcggcttca ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac
    11821 atcaatagca aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca
    11881 ttgtaaatga tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc
    11941 gcgatacccg atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc
    12001 gttggcacgg tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa
    12061 agttcaatag aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat
    12121 tgtttagcca atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca
    12181 gttggattaa cagataaata agacatcact gcgatgatac cagggaatac accatccact
    12241 ttattcagct tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt
    12301 agtgtgacat agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa
    12361 cttatctgtg ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa
    12421 gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac
    12481 cttaaattat tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa
    12541 ctcattccag atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt
    12601 agtcagcgct tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa
    12661 aacaaaaatt catctgatgt atttatcata aaatacatca gatgaactgt caattagttt
    12721 ctttttctcc tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg
    12781 catcaaacgc agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat
    12841 cgatcgtctc gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt
    12901 cattaatgat tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg
    12961 tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa
    13021 tcttttgcgt taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca
    13081 aacctgccat cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat
    13141 cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga
    13201 caaatgtccc cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag
    13261 cttctcgcac cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca
    13321 gtttatctcc tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta
    13381 cttctactag ggttttattt tcttccaaac cacatacctc cttttcatct gatgtatttt
    13441 ttcgtaatta ttataacacg tttgaatcat ctgatgattt tttgtataca aaattgatta
    13501 atccgttcat agacatacaa acgaagaaga tatttaccat aaaaaagact aggataaaat
    13561 tcactcctag ccttcgatca tttttatttt gaacgacaaa aaatatgatg ttcaataaca
    13621 gacgatcttc attcgattct tgatgtcatc ttttgttttt tttaatatat ggataaaaag
    13681 tttgattaca tcgtttctat tctagaattt ggtcgattct caattcttct tctggtgaga
    13741 aatctaaatt atccatcatt cgaacacttt cttgtaactg acttaaccga cttgctccga
    13801 ccagtacact tgtaacagat ttttgtcgca aattccatgc aagggccatt tgcgctaatg
    13861 attgtcctcg tgatacggcg attgtttgca gtgctttgat tttttccaga gttgatccaa
    13921 cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc tctagaatct tcaggaatac
    13981 catgtagata acgatttgtc agcaaccctt gagcaagcgg actgaacgtg attgccccta
    14041 gcccttcttc ttctaatact ggagatagat cgtcttctag tagtgcacga gaaaacatat
    14101 tgtagcgcat ctgatgaata atgaacggtg cttcctttct ttttaaaatt tcagtcattt
    14161 tttttgtatc ttctccatta tagttggata taccaatata taatgctttt ccttgtctta
    14221 caagttgcat caaagcttct gctgtttctt ccaaaggtgt atcagggtct gggcgatgat
    14281 gatagaagat gtcaacataa tctagcccca ttctttggag actttggtca caacttgaaa
    14341 tgatggattt acgagagccc cattccccat atggcccttc ccacatatga taacctgctt
    14401 tgctagaaat aatcagttca tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa
    14461 agtttttctc tgcagctcca gcagggcctc cataattatt cgcaaggtcg aaatgggtaa
    14521 ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt atggatagga tcataatcac
    14581 caaaattttg ccataatccg agagataaaa tcggtaattt caaacctgaa tgaccggatt
    14641 tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt tctcctcctc
    14701 atcaaatctt tttaaaacgc tttctttttt ctatttttag tttactacat tttaaagaaa
    14761 aggagttaat caaaatgatt ttttcgtata tttttttgcc atttgttttt tactcgttga
    14821 tagatcagaa attgattacg tgcttgctgg atttttttat aacgtttcgg ttgttttttg
    14881 taaaattgct gatgatagtt ttctgctggc caaaacgtac cggctggttc gatttttgtg
    14941 acgattggtt ggtggaaagt tcctgaactg tctagtttct gttttgattg ttccgctaat
    15001 tctttttgtc gttcgacttg ataaaaaatc actggacgat attgcttccc tctatcttga
    15061 aattgtccaa atgtatctgt gggatcagta atttgccaat agattgtcag caactcgcta
    15121 taacttattt ctcttgtgtc ataaatgatc tccacagctt ccacatgacc tgtgtaacca
    15181 ccacttactt gatcataatt tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca
    15241 gataggatcc cttttttcga ttcaaacggc tcgaccatac accagaaaca tccccctgca
    15301 aatactgcac gttcttgata gtcagcatct tgcatggcat gcttggaaaa gtcatatttt
    15361 tgctccttat caaatccttc cgtaatcaat aaataaaagt ccattacatc aggcgtcaga
    15421 ttcattcgga tagctaatgg acgtagtgtt gcttctagtt ttgataactg ttctttaacg
    15481 gatatatttt ctcttatgtc attctttgtc tgaatcaata cttttcgttc ccaatctcgt
    15541 gtattcggat tcaaaatcag attataaagt gtttgaatga atatttgttc ctcatccata
    15601 gattctcctc ctttccgaag aagacaagct agtgacttta gctgtaattt atcataaagt
    15661 gaggaaataa gcgattactt catcccccta cagatttttc gtataaaaaa gagagaatcc
    15721 ttatcgacag attctccttt ttaatcaatt tattttataa atgaaaagtt aatttacctt
    15781 ttatggaaaa ctattttaaa aaataaacat aaatatgcag ttaatgtaac aagataagaa
    15841 ataatagtcg ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg
    15901 cttatttgta ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct
    15961 acattcgttc ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt
    16021 actctatata tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca
    16081 atctttgctt cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa
    16141 tcatataaaa aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca
    16201 acaatatgtc ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt
    16261 aaagtgatta ataacaaaac agtcataaat aaataaaata gttctcttgt cttattgtat
    16321 cttctatttt tgaaaaatat catcgttaat atcagagaag agaatattat ttgcaagctt
    16381 aagatccgcc aaggaaattg taacaatttg gctggcgtat tttgcagaag ataccatggc
    16441 accaaactag ttgtagctag ccaagtcaaa cacgtaccat aataaatctt tttttctatg
    16501 tttgttaatt ttttaaataa aatcagggga atacataaag ataacagacc aactagtccc
    16561 attgagtaag tagttaagtc gttgttcata gcttcagtca gactatcgaa cacattagat
    16621 gcatgagcat ataaatttgt cacgctaggt ttgttgattt tttggtagag catttgttca
    16681 aacatcggat accaaaaagc actcgtcaga accaatgtca ttcctgccgc actaaataat
    16741 tttttaaata tagcaataaa atctgcattc ttgaaacctt tagaaaccaa acgaataaaa
    16801 ataaaaatga taattaatgc tgtactcatt aatgttgata gtacatggct gtacactagc
    16861 aaactcatac tcaatgctag ctttacagac ggatactttt cttcttttcc agagatgatt
    16921 tgataagcat aaaataatac agggataaca agtgttattg cgatagcttc ggcaattgcg
    16981 gaacgatagt agatgtctac tgtccgataa gcagagaaaa tatagagaca actaaataaa
    17041 aataagtagt tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa
    17101 ttgcaactga atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca
    17161 ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa
    17221 tgcaaattgg tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta
    17281 tgcaatttct tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc
    17341 aagtaagggt gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag
    17401 tggatgaatt cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca
    17461 agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga
    17521 caatgaccaa gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc
    17581 cgatttcaaa cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac
    17641 gatttgcaac aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt
    17701 ctgaatatcc cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag
    17761 aacagcacaa attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt
    17821 ccaaagataa aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat
    17881 cgcaaaactg tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc
    17941 ttatagaaat caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct
    18001 gctcggaaga agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa
    18061 gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac
    18121 caaaaagaga agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg
    18181 ctaagatggt tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag
    18241 ttggaaccta ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac
    18301 gatatgagac acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca
    18361 agatgcacta ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg
    18421 ggtttgaaga ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat
    18481 atgtgttccg ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag
    18541 atacaagaaa attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag
    18601 attggtctta ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc
    18661 ttcacgaatt agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg
    18721 ctccttatac tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat
    18781 ggcgaatgat tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga
    18841 aagaattaag gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca
    18901 gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata
    18961 tcagaggatt caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac
    19021 taacaagtca tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat
    19081 atgccgataa ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg
    19141 attgtagaac ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg
    19201 ttaatgacca ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg
    19261 aaagaccatt aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc
    19321 cgaagtatgc tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt
    19381 ttaaatcagc ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac
    19441 aatttgattg gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa
    19501 gctcttgtgc tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt
    19561 tttttctttc atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt
    19621 tttgggcaaa tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt
    19681 cgaattcatg atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat
    19741 aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat
    19801 cttctattga atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa
    19861 taatatttct tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata
    19921 ttatcggatg tccctttgta gtatttttaa catattccca gtccttggga atcctccaag
    19981 gaatgtcatt ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt
    20041 tcatttataa cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa
    20101 accttttctc attatcctgc ccgttagtt
//
LOCUS       DAJSNV010000049        19848 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSNV010000049 DAJSNV010000000
VERSION     DAJSNV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.050"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(10..216)
                     /locus_tag="ON516_001668"
     CDS             complement(10..216)
                     /locus_tag="ON516_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3345154.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(349..1308)
                     /locus_tag="ON516_001669"
     CDS             complement(349..1308)
                     /locus_tag="ON516_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3345155.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1574..1918
                     /locus_tag="ON516_001670"
     CDS             1574..1918
                     /locus_tag="ON516_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3345156.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1919..2338
                     /locus_tag="ON516_001671"
     CDS             1919..2338
                     /locus_tag="ON516_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR3345157.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2430..2780)
                     /locus_tag="ON516_001672"
     CDS             complement(2430..2780)
                     /locus_tag="ON516_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345158.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2746..4077)
                     /locus_tag="ON516_001673"
     CDS             complement(2746..4077)
                     /locus_tag="ON516_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3345159.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            4333..4899
                     /locus_tag="ON516_001674"
     CDS             4333..4899
                     /locus_tag="ON516_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3345160.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            5044..5131
                     /locus_tag="ON516_001675"
     tRNA            5044..5131
                     /locus_tag="ON516_001675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5076..5078,aa:Ser,seq:cga)
     gene            5465..6439
                     /gene="bsh"
                     /locus_tag="ON516_001676"
     CDS             5465..6439
                     /gene="bsh"
                     /locus_tag="ON516_001676"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3345161.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6764..8659
                     /gene="asnB"
                     /locus_tag="ON516_001677"
     CDS             6764..8659
                     /gene="asnB"
                     /locus_tag="ON516_001677"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3345162.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8854..9300)
                     /locus_tag="ON516_001678"
     CDS             complement(8854..9300)
                     /locus_tag="ON516_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3345163.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            9528..9677
                     /gene="rpmG"
                     /locus_tag="ON516_001679"
     CDS             9528..9677
                     /gene="rpmG"
                     /locus_tag="ON516_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3345164.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9765..10283
                     /locus_tag="ON516_001680"
     CDS             9765..10283
                     /locus_tag="ON516_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3345165.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10360..11316)
                     /locus_tag="ON516_001681"
     CDS             complement(10360..11316)
                     /locus_tag="ON516_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3345166.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVSSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            11482..12288
                     /locus_tag="ON516_001682"
     CDS             11482..12288
                     /locus_tag="ON516_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345167.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12285..13274
                     /locus_tag="ON516_001683"
     CDS             12285..13274
                     /locus_tag="ON516_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3345168.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            13271..14275
                     /locus_tag="ON516_001684"
     CDS             13271..14275
                     /locus_tag="ON516_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3345169.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            14407..14949
                     /locus_tag="ON516_001685"
     CDS             14407..14949
                     /locus_tag="ON516_001685"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3345170.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14969..15667
                     /locus_tag="ON516_001686"
     CDS             14969..15667
                     /locus_tag="ON516_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3345171.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15688..15900
                     /locus_tag="ON516_001687"
     CDS             15688..15900
                     /locus_tag="ON516_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3345172.1"
                     /translation="MESIYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15900..16862
                     /locus_tag="ON516_001688"
     CDS             15900..16862
                     /locus_tag="ON516_001688"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3345173.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16880..17275
                     /locus_tag="ON516_001689"
     CDS             16880..17275
                     /locus_tag="ON516_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3345174.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            17438..17803
                     /locus_tag="ON516_001690"
     CDS             17438..17803
                     /locus_tag="ON516_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3345175.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17800..19611
                     /locus_tag="ON516_001691"
     CDS             17800..19611
                     /locus_tag="ON516_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3345176.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19673..19840)
                     /locus_tag="ON516_001692"
     CDS             complement(19673..19840)
                     /locus_tag="ON516_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR3345177.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6337 a         3404 c         4033 g         6074 t
ORIGIN      
        1 tctatcctcc tatttatctg atgaaatttt cttatacgtt cgatttgata cacctaaaat
       61 actccctaga aatgctccaa aacctgtaat aatcatcacc gttatatctg tgtaccgcca
      121 gttaacacct tttccgacaa gaccgacaaa agtcgctaaa gcggggataa ctactatagc
      181 gatccatttc aaaatatcaa aggttctatt gttcatacta tccacctccc ttcattggtt
      241 gggctacttt ctttttcaat cgcttgtttt cttctatcaa tatttctctt tctgaaagca
      301 aggcatcaat ttgcccgtcc agttgttcaa ttttatcttt cttaattgtc aaattaagtt
      361 agacagatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataatccag
      421 tgattttcta ggaatattat ttcgtttaga tgcgacagat tgaataaaaa attcatctac
      481 tgaattgaaa tccatagatt tcaataaacc atctctacgt aacaatccgt tagaattctc
      541 gtttaggcct ctttgagacg gcgttcctgg atcagcgaaa taaatggcaa tatcattgac
      601 attactgatc tgtttccaat ttgaaaattc ctttccacaa tcaaaagtga tggatttgaa
      661 taggtttttc ggtacagatt caaaccattg atttaatttt ttttcaatat caatcgcttg
      721 tctaccacac ggtttcaatg tgatgataac ttttgataat cgttcaacta aggtaattac
      781 agcactttta tgtttcagac ctacgatagt gtcaccttca aggtgaccaa actcatttga
      841 gaattggcta taatcctttt cacgttcatg aatagagcgg cggaaagttt gttttccacg
      901 tctttcttga tgtccattcg gtttacgctt gcctttcatc ggtaagtcag aagaatcaaa
      961 tagccccttt ttgaacatac gataaatggt acgagcagaa caactaatag gaaacgctgc
     1021 acgaccaaca atcacatcgg gtgtccatcc ttgaacaacc tttctttgaa tatattctga
     1081 ttgttcctcg ggtaaaacaa gcggacgtct accacagttt gatttgtttt tcttatactg
     1141 ttgataatat tctaaggctg attttccttg cttgaagaac agatatactt tatggatcgt
     1201 ttgtcttgaa cgattcaagc aatgggcagt tttagcaaca gattgattca ttttgaaata
     1261 agactctatt attacaagtt catccgttgt aagatgggta taggtcattt gtactcactc
     1321 cttataattc tttggtcgga actattttga gtgtagcaca aatgactttt ttagttgtct
     1381 agcttaattt tacaatcggc gaataaaaaa agcgttagca actatgctta aaaagcatat
     1441 cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg aaatgcgaag
     1501 aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca gtcgtgaagg
     1561 gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag gagattttgc
     1621 accaaaattt gcagaattga atgatgatgt tttatttgga caagtatggt ccagagaaga
     1681 attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag gaggaaattt
     1741 tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta gtgaagaaat
     1801 agcagaaatc attacccatt tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt
     1861 tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag aggcgtaaat
     1921 gacgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga
     1981 agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga atgatcccga
     2041 agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc actggcatgt
     2101 ccataaagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt atcaggaaga
     2161 aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca aagggattaa
     2221 acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca ctgctggtaa
     2281 ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa atcaataatg
     2341 tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc tgcttttttg
     2401 attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc acgtcgaaag
     2461 atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat caaactacct
     2521 aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg ataatagatt
     2581 tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa atcttctggc
     2641 tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa ttctactcca
     2701 atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc gatgattctt
     2761 tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa agttatctag
     2821 tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat atagatttag
     2881 tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagtacac cagctattac
     2941 accgtaaaaa ctgccgtaaa aaccgcttat gggcgcgaga cctgtcccaa acacagctgc
     3001 aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag caataaatat
     3061 tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga aacccaccgc
     3121 agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca aaacataaga
     3181 agacagcatc agaccaacta agcctatgtt gatcaaagta gcaccaatac cagaattagc
     3241 aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg
     3301 aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa aaataatcca
     3361 aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg aatagctgaa
     3421 taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc caatattata
     3481 aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa gaagtagacc
     3541 aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa aacgcatacc
     3601 aaaagtgata taactaacga ccggtgataa tgcgctacca aacagtccga tcatgatgta
     3661 ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa tgattgagat
     3721 agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa
     3781 taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa
     3841 aatccctaca tttatgaatg cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag
     3901 attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag gagtattaga
     3961 aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat actttctttt
     4021 ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt tttccattac
     4081 acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg acaactatca
     4141 tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt tttttaattt
     4201 tgcaaaaaaa agttaaagaa gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat
     4261 gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca gtaaagaagt
     4321 gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa agctgcttat
     4381 gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc taaaaagatg
     4441 gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg
     4501 ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga acgtactggt
     4561 gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca tcatagtctt
     4621 tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga tttttcatac
     4681 aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc ggatgaatat
     4741 gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc attgatgtca
     4801 tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg gacaattgac
     4861 tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag tatgaatttg
     4921 atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa aagcgatttc
     4981 tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa aaatagtact
     5041 tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac cggctaatac
     5101 cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt tgtgaagaag
     5161 ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta ttttttcgtt
     5221 tgattgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg aataaggggc
     5281 tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca atattttttg
     5341 attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt ggcctttttt
     5401 tgataaaaaa aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggacggga
     5461 aacgatgtgt accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga
     5521 ttatgagatg tcttataatg aagtggtcac tattactcca agaaattatc gtttcgattt
     5581 tagaaaggta aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc
     5641 caactatcca ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa
     5701 ttttcctggc aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt
     5761 tgagtttatt ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt
     5821 ggctactatc aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca
     5881 ctggttatta gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca
     5941 tttatatgat aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt
     6001 taatctgaac aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca
     6061 gatcagcttg aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc
     6121 ttctgtttct cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc
     6181 agaatctgaa agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg
     6241 agtatgcgat actggagaaa gaaaatacga atatacgatt tattcttctt gctgtaacgt
     6301 tgataaaggg atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat
     6361 gaataaagaa gacttggatc gtcatgaatt ggtcagttat ccagtcataa aagagcaaca
     6421 gataaactat gtcaattaat taatgaatag atccgtttgg tatcatattt tctgatacag
     6481 tcataattag acacgatctt taggtcgtgt ctttttttgc tggttttttt ggaaatagca
     6541 tccactaata tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta
     6601 aattagatat tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg
     6661 tgacgaaatt gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat
     6721 gaatgaaata aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg
     6781 ttttgttaac gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt
     6841 tcaccgtgga cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag
     6901 aagattaagt attattgact tagagggcgg tactcagcct atttacaatg aagatcggac
     6961 aaagatcatt atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat
     7021 tgcagctggc cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga
     7081 agaatgggga acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga
     7141 taatgaaaag aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta
     7201 tgcagaaatg aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc
     7261 tgattttaac aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc
     7321 tccattaaac ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta
     7381 tacgtataaa gatggaaaaa tagacattca gcaatattgg gatgcagatt ttgaaacaaa
     7441 agaaacgcat tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat
     7501 tgaagcacat acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc
     7561 cagttatgta acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa
     7621 aacctataat gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac
     7681 agcagcagtg atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt
     7741 agatgaacct gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc
     7801 acaaagtgtg cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca
     7861 aacgtatgga ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa
     7921 attaccaaaa ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaatttcca
     7981 tggacggatc catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc
     8041 tcgagtgttc gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc
     8101 atctgtggat gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt
     8161 caataagatg caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc
     8221 agataaactt tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt
     8281 catgaaattg gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga
     8341 tattttcaga caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt
     8401 aggattccct gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa
     8461 agcattattc gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt
     8521 gctagatgac catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag
     8581 tttcttgaca tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat
     8641 cgactatgtg actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg
     8701 tttaacactg agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt
     8761 atgacttatg gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg
     8821 acacgatttt cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt
     8881 cccatctttt ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt
     8941 ttcgattgct tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt
     9001 cacgatccat tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc
     9061 acgaggattc gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg
     9121 tttttctgat tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat
     9181 cgcacggtta tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt
     9241 tcttgtcaat ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat
     9301 attagtatcc ccctttatta aaaaacataa tctatataaa caagtgtaag ggtatttata
     9361 caaaaagaaa aagattttgt ttgagaagta aaaccgttgt atttatcgtc aagtagtttt
     9421 acttaacaaa atcgtgaaaa acactagcaa tctagaaaaa atcatggtaa gataatcaag
     9481 tctgagagtc atcttagagg attcttgtaa aaatgggagg gaaaaacatg cgcgtaaaca
     9541 tcactttaga gtgtacttct tgtaaagaac gtaactatct aacaagcaaa aacaaacgta
     9601 acaatcctga tcgtttggaa aaacaaaaat attgcccacg cgaacgcaaa gttactttgc
     9661 accgtgaaac aaaataatgt ttttaaagtt ctcaattcct tgctggtcaa agaattgaga
     9721 ctttttttat ctcaaaacat ctaaaatcat aaggagcgga taatatgttt atttccatgt
     9781 gggcacaaga taaaaatggt ctgatcggca aaaatggttt gttaccatgg cgattgccaa
     9841 atgatatgcg tttctttcgc gaacacacaa tggatcgaat tttagtgatg ggaagaaaga
     9901 cctatgaagg aatgggggat ctttcacttc cttatcgcca tatcatcgtg ttgacaacgc
     9961 aaccaaattt tcgcacgaaa gaaaatgcgg aagtaatgca ttcgatcgat gaacttttgt
    10021 cgtatagtca aacgatctca gaagatatct atgtttcggg tggtagtcgg atttttcaag
    10081 aacttcttcc ttacactggt cggatctggc gaacattgat cgatagcacg tttgaaggcg
    10141 atacgtatat tgggaatatt gatttttcag acttcgccct tatagaggag tatgagggga
    10201 taacggatca agaaaatcta tatgcccatc gttttcaaaa atgggaaaga gtaaaaaata
    10261 gggttgcttc aaaagaagaa taaaatctat gcgtctagtt tgttaataag aaaaaaagca
    10321 tctcacgact aatcgtgaag atgctttttt tagttaaaat caatttagtt tttctttgat
    10381 atcattgacc atttcctgac tggcaatagg tccgttatat agccaactag attcaggtcc
    10441 gaattgcagt acatgattgt ctgtcactgc ttttagattc ttccagcttg gttcattgaa
    10501 catggcagca tctttgtcac tgctcacaag gatgatgtag tcagcatcta attcagctag
    10561 cttttcagag gaaacagcag accagtcaga agtggctttt ttggagacct cttccactag
    10621 atcaggaaca ccaaattcca gatcgtcgta tacgattctt ccacttgacc gatttttgct
    10681 gaccataaag gctgagttgt tggtcaccca caacactgca gctgtcttgt tctcgatttt
    10741 atctgctagt tcttctcgag ttttcttgat ggatttttga taatcggaaa tgattttttc
    10801 tgcctctttt tcttttgcca aagctttgcc gacatctttt agttgttctt cccatgtgac
    10861 atctgttccg ttttttacaa cataagttgg cgcgattttg ctatattgtt cgtatttccc
    10921 gccttcgaca gtagcggaag aagaaatcaa cagcaaatca ggctcaaagc ttaacacttt
    10981 ctcataagga aggtcataag aaatcgttgg gacatccttt aaatcatctt gcagataatc
    11041 ttgaatggaa ccactgccaa cagtccactg ggcgataggt ttttccccta gtgcaatcag
    11101 atagtcttct aagtaactgc cgatgatccg tttgggcgcg tttggaatct ctactttatg
    11161 tccaagtgta tctgtgactg tatgagtact attggatgaa gtcgtcgaat tggcagttgt
    11221 agctgtttct ttatctgaag agggggagca agcgctcagt actccagcca gcagcaatat
    11281 gctagataat gtgaatactt ttgtttttaa tttcatttct ttcctcctaa cataattgag
    11341 aatctttctc attggttatt attcctgata tttcgaacgc tgtcaaccgc ttcatttggc
    11401 tatagatatg aaagtagcgc ttgacgaaat gttgaaacag cgtcataatt gagaatgatt
    11461 ttcagcgaaa ggatgggtga aatgacgaga ttaagagcag ctgacattgc agccggctac
    11521 gaaaacaaaa aaatcattga aaacatgtct gttacgattc ctgaagggaa aatcactagt
    11581 ttgattgggc caaatggaag tggaaaatct actttattga aaagttttac ccgtatacta
    11641 cctgtgaaat caggaaagat cattgtcaat aactctgata tctctacata cagttcaaaa
    11701 gaacttgcaa aaaaaattgc attattagca caaactagtg aacaccctac aggtatgaca
    11761 gtagaagaag tagtatctta cggacgatac ccttatcaaa aactgttcag tggcatggac
    11821 acgcaagatc atgaagcaat tgagtgggcg ttggatgcta caggtctagc tggatttcgt
    11881 atgcgggata tcgcaacgct gtctggtgga caaacccagc gtgtctggat cgctatggcg
    11941 cttgctcagg aaacagatat cttgatcttg gatgaaccga caacattttt agatccggca
    12001 caccaattag agatcctaca gttattgact gaaatcaatc aaatgaaaca aacaacgatc
    12061 ttgatgtcga ttcatgacat taatcatgct tcacgttttt cagattattt attagcaatg
    12121 aaaaacggta agttgatcac agaaggtaca cctgaagaag tgatcacaaa agagtgtatg
    12181 aaaaaaatat ttgagattga tgcaacgatt gtcacgcttc caagaacaaa taaaccagtt
    12241 tttctaacct atgatttgtt gccaaaagag caggaggatc aattatgagg actatgaaga
    12301 aaaatagact cctgcttttg gtctgttttg ttcttttgat tatttttttt gttgctcttt
    12361 caaatggcgc agcgactgtg ggaaaagcag atttttggca ggcgttgttt gattttgata
    12421 gatcaaaaca aagtcaccaa atcatacgaa atattcgttt gcctcgtgtg gcgggagcat
    12481 ttcttgtagg gagttgtttt gcattgagcg gtgcattgat gcaaggagtg acaagaaatc
    12541 ctttagcaga ttcaggctta ctaggaatca acgcgggtgc ttcgttggca atggccctta
    12601 gttttgcttt ttggcctaaa gcttctgatt tttctgtgtt tctaatgtca ttggcaggtt
    12661 cttttgctgt aactttgctc gtatttggtt ttttgcagtt ttcgaaaatg ggcatgaatc
    12721 ctttacagct gattttagct ggcgtagcta ttagttcttt ttttactgct atcagccaag
    12781 cacttaccca attctttaat ctgcagcagg atcttgcttt ttggtttgta ggcggagctg
    12841 ccaatatcac atggaaacaa ttagcagtat tgtttccttt atatatactt gctgttttgg
    12901 tttctttttc attaggaaaa gcaatatctt tgattcattt gagtgaaaca catgcattag
    12961 ctcttggaag tcatcctaaa cgaacgcggt tgattgtttt ctgtctagtt gcagtgttag
    13021 cagccattgc cgtttcgtta gtcggacctg tttcatttat tgggctgttg gttccgccag
    13081 ttgttcgcgg aatggttgga acggactatc gttatgttct acctgtctct tttttagttg
    13141 gcggtggatt ggttatgttg gcggatttta cagcgaggat gatcaatccc ccttttgaaa
    13201 caccttttgg attagtcatt gcattgattg gcgttccgtt tttactgata caagtaagga
    13261 gggaacaacg gtgaaaaaaa tatattggac gttctatttc ttgctttttg tacttgtcat
    13321 cctgtctata ggtagtttaa tgattggtac gccttttatt tacctggatc agctttttgc
    13381 cacgttcata gggaaagaat catcttcttt ggcgctgatt gttagagaat ttcgagttcc
    13441 tcgattactg atcagtttat tagcaggagc ttgtcttggc gtttctggtt atatcttgca
    13501 aggagtaaca agaaatgaat tggcagattc tagtatattg ggcattaatg ccggagcagg
    13561 atttttagtg atgctttatt taggtttctt ttcgcactat tcgctgccgt ttctattgct
    13621 agttgtagcc tttctaggtg gtgttttagc tgcttgctgt gtctatcttg cggcctatga
    13681 tcgaaatggc tttttgggta tgaatcgaat cttattgtcc ggtattgccg ttaatgccgg
    13741 cctttctgca ttaacattgc tatgcacgat ccaattatct aaggaaaatt atggatttgt
    13801 gaatgcatgg ttagcgggat caatctgggg tgcgagctgg ccttatgttt gggcattgct
    13861 tccatgggcg gttatcttaa ttccgctagg aggactttat gcacagagga ttgtattgct
    13921 atcatttggt caagaacgcg cgcaaggatt aggccttaac gtggctaaaa gtcaaaaaat
    13981 ccttttgttg ttagctgttg ctttggcaag cggtagtgta gcggtgacag gtagtttatc
    14041 ttttgtagga ctactagctc ctcatgctgc gaagctagtc gtacgtaaag aaaataactg
    14101 gacctttgtt ctgagcggtt tgttcggttc catttttctg cttagtgcag atatggttgc
    14161 tagagtgatt ctactaagtg gggaagtacc tacaggaatt ttgattgccc tttttggtgc
    14221 gccatatttt ctgtatgcac tttttatcag acaaaaacac gcgctgaaca gttgaaattc
    14281 attaaaaaag gaaggccaat catttctagg tcttcttttt attctattta tgcatttatt
    14341 tgaaagttcg attgctattt ttcttttttt cctataccat tttatgagag aaggaagggt
    14401 gagtttttgg accaattgaa agtaagatta cgccggaaag gaatcgaaaa gctgaaaaat
    14461 ctggccaaaa gtggcaagaa aaaggaaaaa gaacagcgaa tctataaaca gctattcgcc
    14521 agcgagtact ggaagtcagc aaaagtgata gggacgactt tatcgaatga atttgaactg
    14581 gatacacagc cggttatcca acgagcagaa aaagaacaaa agaaaatagt tgtacctaaa
    14641 actttgccga aacgtcagat ggcgttttat gaaatggatg aacagtccgt tcttgtacgt
    14701 tcgagttttg gtgttcttga gccacaatca gagaagctct atttgccgga gatgatcgat
    14761 ctcctcattg taccaggtat tatctacaat agagccggtt atcgaatcgg ttttggcggt
    14821 ggttattacg accggtattt aaataatttt cataaaaaaa catgtagcct tgtttttcgt
    14881 gaacaattga atgaacaatg gcaaccggag agctttgatc agaagatcca acaattgttt
    14941 attgactaaa atgaaatggg aggttttcat gaattatcaa cagcaaataa aattgcgtgc
    15001 atggttaaga cgaccgttat ggacctatgc ttttttaggg atacagacag cggtcttcct
    15061 catgatggaa ttatttccgc gacttgagat cccttattat gccggaatgt atggtcctta
    15121 tcttgttcac ttcaatgaat ggtggcgttt agtgacacca atcttcatcc attttggact
    15181 gatgcatttc gtaatgaact cactgatact gtattttatg gggcagcaaa tcgaggcaat
    15241 ctatggccat tggcgttttt tcttgattta tatgttcagc gggataatgg gaaatacagc
    15301 aagctttgct tttaacgaag cgaatgttct gtctggtggt gcaagtactt cgatattcgg
    15361 tttgttcggt gcgttgttta tcttagggtt tcattttaaa cataacccag cgatccagca
    15421 gctagtcaga cattttcttt tatttattgt gttgacattt gtttttggtc ttctggatac
    15481 ctctgtagat gtttggggac atgtcggtgg gctaattggc ggattagtgt tagggaatgt
    15541 tttgggtcta ccgaaacaac agacaagcta ttcgatacac caaaggattc tttctacact
    15601 agtttttgta tttctttttg taatatgtat tttactgggt ttaaaaaaat atggtttact
    15661 tgtataatat gaaggaagtg cttaaagatg gagagcatat atgatgtaca gcagctgttc
    15721 aaaaaatttg gcatctttat ctatgtgggt gcacgcatct atgacattga attaatgact
    15781 atcgaattgc gtaatctgta tgatagccgt ttgattgatc atgatacata catgacagcg
    15841 tggcgcatat taaaaagaga gcaccgaatt gaagaaagcc gaaagaaagg aaatttgtaa
    15901 tggacaagaa gattattggg attgatttag gtggaacaac agcaaaattt gcaattttaa
    15961 caccagaagg agagattcag caaaaatgga gcatcgatac caatattttg gatgatggta
    16021 agcatatcgt tcctgaaatc attgaatcaa tcaatcatcg tttgaattta tataatatga
    16081 aagccgaaga ctttatcgga atcggcatgg gtaccccagg aagcgtggac agcgaagctg
    16141 gaacggttat tggtgcgtat aacttaaact ggacagaagt tcaatttgtg aaaaaactga
    16201 tcgaggcagg aacaggaatc aaattcgcta tcgataatga cgcgaatgtt gctgctttag
    16261 gtgaacgatg gaaaggtgct ggagaaaacg atccagacgt tgtattcgtt actctaggaa
    16321 caggtgttgg cggaggtatc gttgctggcg gaaatttgat tcacggcgtg agcggagctg
    16381 gaggagaaat cggacatatc actgtggatc ctgaagggtt cgaatgtact tgcggtaaac
    16441 gcggatgttt agaaactgta tctagcgcta caggtgtggt acgcctaggt cgtttcttgg
    16501 cagaagaata cgcaggggat tcaaaactga aagcaatgct agacaacgga gaagaagtaa
    16561 caagtaaaga tatctttgaa atggctcaag aagacgatcc gtttgcattg atggttgttg
    16621 atcgcgtatg cttctactta ggtttagctt gcggaaactt ggggaataca ttaaatccgt
    16681 caagtatcgt gttaggtgga ggagtatcgg cagcaggaga atttttacgt agccgtgtag
    16741 agaaatattt taaagaattt actttcccgc aagtaagaga atcaacaaaa atcaaattag
    16801 cagagcttgg taatgaagct ggtgtcattg gtgctgcctc attagccttg aaatttgctt
    16861 aaaaaaacgg aggcattaca tggttttatg ggtaattaat atcattttgc tattgattgt
    16921 gcttgcaatc atcctttggg aagtctatct acgagtaatg gccaaacgtt cagcaacgac
    16981 tttgacagaa gaagaatttc gagaaggaat gagaaaagca caagtaatcg atgttcgcga
    17041 aaaagatgtg tttgatgctg ggcatatttt aggtgcaaga aacatccctt acactgtttt
    17101 gaaagattca ttaggatcta ttcgaaaaga ccagcctgtc tatatttacg atcaaaagaa
    17161 aagtttgagc atccgtacag caaataaatt acgtaaagca ggttatcaag agatttattt
    17221 acttaagggt ggatatgacg gatggtctgg aaaaatcaaa agtaaaaaag tataaaacag
    17281 atgattgtat gacagcttta aaaaccgaat caatcattag attttgacca agatcacatg
    17341 aacggcgcaa tggatagttg tgccgttcat tttttttatt ttgtgctatt atctacatat
    17401 ataagctaag cgaaaaggta gaaagaagga aacaactatg atcaaattaa aacgtgttta
    17461 ccttgatgct gaaaaagagg atggctatag aatactcgtg gatagattct ggccacgagg
    17521 aatttcaaaa gaaaaagcgc agttagatct gtggttgaaa gaaattgcac ctagtaatga
    17581 agcacgcaaa acttttgacc atgtaccaga acgatttgct tcatttgcaa agaaatacca
    17641 gcaagaattg attttcgaag aagaaaagaa gcaagctatt tttaaactca gtcaacttat
    17701 gaaagaacat ccagtcatta cttttgttta tggtgcaaaa aatgaacatc agaaccatgc
    17761 tgttttattg aaaaaattta tcgaaatgga ggcatgcaaa tgaaaatagg gatcattggt
    17821 gccggaccta gaggattaag tgtagcagaa aggattctcc ataataatca ggacagacat
    17881 tctcttgaac tttttctttt cgatcccaat ggtcctggag gtcgagtttg gcggttgaat
    17941 cagccaccgg agttattaat gaattctgtt tctcaacaag tgacgctttt tacagatgaa
    18001 acattgtcca gtggaggatt ggtaacacag ggacccaact tgtttcaatg gagtaaagga
    18061 cctgctacag attatatcga acagacggta accaaaaata agcaactaat tatagaagaa
    18121 atcaatcgtc tggaaaaaga tcagcaatct actcgatgtt tatacggact ctatcaacgc
    18181 tggtttttct caaagttgca agaagaattt ccagcttcta ttcagtttgt ttattcttta
    18241 gtaaaaaaag taagcaaaag aaaagatggt tatcttttac agacagatca gcaaagctat
    18301 tctatcgatc agcttgtttt agcaagcggt cactgggaaa atgatgagac agaagaagaa
    18361 agagtgtggg tagactacgc aaagaaaaat gttttactgt atcagcttcc ttctaatcct
    18421 gcagatgtag atctttcgaa gataccgcct aaagaaaatg tctttttaag aggtctaggc
    18481 ttaagttttt ttgattatgt cggattgttt acagtatcaa gaggagggcg cttcgagaac
    18541 gaaaagggaa aactggtcta tcatccatca ggcaaggaac cgattgtata cagtggatca
    18601 agaagggggc tcccttatta ccctcgagga agaaatcaaa agcaaggcgg agcaatggca
    18661 tggccacggt tactaacaaa agaaaacctg gagcaatggc atcagtcggg atttttgact
    18721 ggcgaaacat tttttgacta tctgaaaaaa gacgttgagt tgttttatta caagaaaatc
    18781 atcgaagaat atcacttgcc gattccaaag aaaaattttg agcatgattt tctctccctc
    18841 tcttcagagg agtgtctcgg aaagcatcca gcactaatcc cttatagatg ggattggtcg
    18901 tatttagaaa ctcctcttgc ctatcagaat gagccctttt cagaagctag ccgaatattt
    18961 attgaagggc agataaaaga agcggaaaaa ggcaattgca caggagcatt tgcttcaaca
    19021 tttgatgcat taaaagactg gcgtgatcca atccggcagg ctattgaatg gcacgttttt
    19081 tctgctaagg aatacaaaga aatactatgg ggatggttta ctcatctaaa tagcttttta
    19141 acgatcggtc cgccaatcat tcggacgaaa gaattagcag cactgataga tgcgggtatt
    19201 tttcatttga tcgaaccgcc atttgatatt cagcaaaagc acaaccgcta tgttatttct
    19261 ctacaagata aagaaatcag cagtcgctat ttgattgaag ctcgattgcc acaaacaagc
    19321 ttttctcgtt caagtaatcc tgtcataaaa aatttgaagg aaaacaaact tgttcgccct
    19381 ttttcatttt cagataatac aggaagttat cattcagaag ctatagatgt tgagcgaggt
    19441 acgaatcgcg tgattgatgc ttcaggggaa atccagtctg acttttattg ctatggaata
    19501 cctgttgaag gtgtggattg gctaactgca gcagtagcta gaccttatac agatccatgg
    19561 aatctgagac aggcagataa aatagcacaa cttatattga ctgacaaata aaaatacgca
    19621 tgatgactta tctgtttttt agaaagtctt catgcgtatt tttaaataat tattaacgag
    19681 aaacgcgttt acgcattgct tttttacggt tttcttcgat cattgcttct ttttcgtcaa
    19741 ttggatcgat cactgttttt ttcacagaag caaccaaacc agcaacggca gcggctgttg
    19801 ccagacttcc tactaatatt ccagatacaa attttttcat tgaatcca
//
LOCUS       DAJSNV010000050        19108 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSNV010000050 DAJSNV010000000
VERSION     DAJSNV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.051"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(182..346)
                     /locus_tag="ON516_001693"
     CDS             complement(182..346)
                     /locus_tag="ON516_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345178.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            641..1969
                     /locus_tag="ON516_001694"
     CDS             641..1969
                     /locus_tag="ON516_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3345179.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2171..3004
                     /locus_tag="ON516_001695"
     CDS             2171..3004
                     /locus_tag="ON516_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3345180.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3020..3757
                     /locus_tag="ON516_001696"
     CDS             3020..3757
                     /locus_tag="ON516_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3345181.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3912..4880)
                     /locus_tag="ON516_001697"
     CDS             complement(3912..4880)
                     /locus_tag="ON516_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3345182.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5112..5945
                     /locus_tag="ON516_001698"
     CDS             5112..5945
                     /locus_tag="ON516_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3345183.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5979..6818
                     /locus_tag="ON516_001699"
     CDS             5979..6818
                     /locus_tag="ON516_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3345184.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6844..7404
                     /locus_tag="ON516_001700"
     CDS             6844..7404
                     /locus_tag="ON516_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3345185.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7603..9324
                     /locus_tag="ON516_001701"
     CDS             7603..9324
                     /locus_tag="ON516_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3345186.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9489..10631
                     /locus_tag="ON516_001702"
     CDS             9489..10631
                     /locus_tag="ON516_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3345187.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10647..11858
                     /gene="thiI"
                     /locus_tag="ON516_001703"
     CDS             10647..11858
                     /gene="thiI"
                     /locus_tag="ON516_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3345188.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12431..13078
                     /locus_tag="ON516_001704"
     CDS             12431..13078
                     /locus_tag="ON516_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3345189.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13207..13423
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13471..16116
                     /locus_tag="ON516_001705"
     CDS             13471..16116
                     /locus_tag="ON516_001705"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3345190.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16426..17739
                     /locus_tag="ON516_001706"
     CDS             16426..17739
                     /locus_tag="ON516_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3345191.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17729..18382
                     /locus_tag="ON516_001707"
     CDS             17729..18382
                     /locus_tag="ON516_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3345192.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18437..19108
                     /gene="radC"
                     /locus_tag="ON516_001708"
     CDS             18437..19108
                     /gene="radC"
                     /locus_tag="ON516_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3345193.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         6426 a         3228 c         4031 g         5423 t
ORIGIN      
        1 gactgttaat catgatgtcg taggttcgag tcctactgcc ggagtaaata aagcttatgc
       61 aaagaaaaag aaatccaatc acttgggttt ctttttcttt tattattatg atgaatcaag
      121 ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag agtagagttg gctactcata
      181 atcaaccata cttcccatca tcgctaaata cgaaataaat cagcattcct acaccaatca
      241 aagcacaagc tgcaccaaga aaagggatga cattcccaaa gttatctgta ttcataagta
      301 tgccaccacc cacaaacaaa aagccgccag caatcaggtt tttcatttta ttcatctcct
      361 ttcttacgtt atagtatttc cttctttcaa tgagtatttt gaaagctatc ttattgtatg
      421 atgttcttct aggcctcttc tttattatac aggccatttt cgttatctca tagaaatatt
      481 cctttttatc tgaaaactat tgtataccgt atgacatttt tcttcatgaa gttgtaagta
      541 tattctcatt caaatgaaaa tttcatcata gaagagattg tatttctaaa ttcttaagag
      601 taaaatacga agtataactt ggatttggaa ggatgattgg atgaaagcta ttacgcctga
      661 tatgacaaca aaatttaaca aacagtttca agcaaatatc aaacagcagg cattgcagcg
      721 ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat gtttcagcac atgtggagaa
      781 tacaccggta ttttctatag atttggctac ggggaaagtg gctaatcaaa tgcaatcggg
      841 gcgttgctgg atgtttgctg cactgaacac attccgtcat aagatgctga atcaattcca
      901 tttaaaagag tttgaattat cacaaaatta cacttttttc tgggataaat atgaaaaggc
      961 aaattatttc tatgaaaaca tcattgcaac aggaaaagag gcaatcgata gccggaaagt
     1021 agctttctta ctcgctactc ctcaacaaga tggcggccaa tgggatatga tcgtttcatt
     1081 attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa
     1141 ttctcgagat ttgaataact acttgaacaa attactgcgt aaagatgcag tagtgttacg
     1201 gaaaatggtt gctcaaggag aaacattaga cgctattgaa gaaaagaaag aagaaatgtt
     1261 agaaagcatt tataacttct tagctatctc attaggaaca cctccaaaag aattcgattt
     1321 tgagtaccgc gatgaagaaa agaactatca tcttgaccga ggactgacac cacaaatttt
     1381 ttatgataaa tacatcggtg taaaattaga tgattatgtt agcgtgatca atgcaccaac
     1441 aaaagacaaa ccattcaatc gttcttatac agtagagatg ctaggaaatg tcgttggcgg
     1501 gaaagaagtc aaatatctga atgttgatat ggaaactttc aaaaaattag cgattgctca
     1561 gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta ggtcaatctt ctactagaac
     1621 tagtggcatc atggccttag atgcttattc aatggatgat ctatttgata cggattttac
     1681 aatgactaaa gctgaacgtt tagattttgg agaaagtctg atgacccatg cgatggtatt
     1741 gaccggtgtt gacttagtag acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg
     1801 cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat gcctggatgg acgaatatac
     1861 gtaccaaatc gttgtaagaa aagatttatt gacgaaagag caattaaatg cgttcaatga
     1921 agaaccaatt gttctatctc cgtgggatcc aatgggtgct ttagcataga cgagtatttc
     1981 aatgaagagt atgtagtaaa gataaaaaga gctggcttaa gccagctctt tttatcttta
     2041 aagtccattc cctgtttttt atggtccctg tgctataatt ctaaataaag gataaggatt
     2101 ggaaataatc cttttatcat taaaaatatg atgacgaagt agaataaaat tgggagtagg
     2161 ggggaaagtc atggatatcc aaactgtttt atctgatctg aaattaaatg gtaaagtgat
     2221 tccagttgtt ggtggcgatg ttaatcagac ctatcgtata aaaaccgaac atcgtgctta
     2281 ttttttaaaa atccatccga atgtaaaaaa aggatttttt gaagcagaag tagacggatt
     2341 aaaagaactg tcagcattcg tacgtgtacc ggatacgtat atgctaggtg aaacaagtga
     2401 aggcgcttat ttgttaatgg aatggatcga gccgggaaaa ggggatcaac gagatcttgc
     2461 tgcagcttta gctaatctcc atcagcaaac agcgccacaa tttggttttc gtaaagataa
     2521 ctatttgggt acacttgttc agaaaaattc ttttgaagaa gattggtgga cttttttctt
     2581 taaagatcgg cttgaatccc agatttcttt ggcagaagaa acgaatcgat ggaatgtaca
     2641 gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa
     2701 gatcactcca cgcctcttgc acggagacct gtggagtggt aatgtgttct ttgaccagca
     2761 agggcatcca gtttttgtcg acccagctgt ttcttatggg aatcgggaac aagatattgc
     2821 aatgagccag ttattcggtg gtttccgacc ggaattttta gatgcttatc aaactatatt
     2881 tccattagaa aagggatgga aagaccgact gccgatttac cagttatatt atcttttggc
     2941 acatttgaat atgtttggag aaagttacgg ttcccaagta gatcaattac tagaaaattt
     3001 ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa aagcatgaag tttcatatta
     3061 tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg ttaggagtag ttatcaaaac
     3121 gtctgaagat caaagcgacc agttgggaag aggcagtgaa ttcgtcaata gctttggact
     3181 tacttgggtc atcacgaatt attccatgga gattaatcgt ctccctagag tcggtgaagt
     3241 aatctcagta acgactcaag ccatggagta caataagttt ttctgttatc ggaacttctg
     3301 gatccatgat gaagatggga aagaattagt caaaatagaa tctgttttcg tattgatgga
     3361 ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc attgctcctt ttgagagtga
     3421 gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt
     3481 gccatatcgt gttcgttttt atgatatcga tagtaatcag catgttaata atgcgatgta
     3541 ttttaattgg atcattgacg tattaggata tgactttttg actacgcatg ttccagaaaa
     3601 agtaattatt cgatttgata aagaagtaga gtacgggaat gagatcgaaa gccattttga
     3661 acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct
     3721 gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa agaggttgtg acagaagggg
     3781 gcaacttcaa gaaataagaa gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt
     3841 cgacttagtt ttcgaagaag ctatttttgt cacagcctct ctttttaagt ttagctatta
     3901 gataatgatg tttagttttg gataaacgaa atcaattctt cacccgacaa accatagttt
     3961 acagcaatca atagttttaa acgtgctttt tgactattaa tactgttaca gaaaataatc
     4021 cccatttttt ttagttcttt tcccccgcct tcataatcat aaacaggctc tgcgattcca
     4081 ttgaaacaac gagaaacaag tactacaggg attttttgga caagaagctt ttccaaagca
     4141 gctaaggctt gtggagggag attccccgca cctaaagctt cgattaccaa accgtctact
     4201 ttcgtatggg cgatggcttc caataagtcc ccttgcatcc cagcatatgc tttaacgatc
     4261 gggatagtcc catctacagc ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag
     4321 aataaaattc gattcttagt tactaagccg atgggaccca atgtaggtgt tcggaaagta
     4381 gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag aatggatctc atcgttcatg
     4441 accaccaaaa cccccttatc tagcgcttct tcacaacttg cgacacgaat cgcactttca
     4501 aaattataaa ggccgtcact tccaagttca ttacttgaac gcattgcccc agtcaaaaca
     4561 attggtaatt tgttgccaat cgttgtatca agaaaaaaag cagtttcttc taatgtatct
     4621 gtaccgtgtg tgatcaccac tccggatata cctgatgcat aagcggacaa gatacgttct
     4681 tttaatacca acatgtgaga aggcatgatg tgtggtgagg gaagattgaa gatatcttca
     4741 actaataatt ctacatgctt ggggattgtg atttctgcat ctaataatgg gtttgccaca
     4801 tcaggactaa caccgcccgt gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt
     4861 agtacaagta tttttttcat tttacagact tccttacgaa taattttgca tagccgttaa
     4921 atcttattgt atactatata aaagagtttt gctatcagtg aagaataacg aatagcctaa
     4981 cttatcataa aaaagctgac cttattaaaa tggaaaagac tcaaccttga cctcctaata
     5041 caatggagta tctggttgag tttcttttac aagaaagcag ccaaggaaat caatcacgaa
     5101 ggtggccgga aatgatcaga atcggattaa catcgtttaa agaacatgac aaattaactg
     5161 gtaaaaagaa taacactctt tatgaatatg cagggtttct acccttagtg gaattagata
     5221 ctgcctatta tggaattccc cgaaagtcct cagttgagaa ttggataagt gaagtacctg
     5281 aatcatttcg ctttgtagtc aaagtctatc aaggaatcag cggtcaagga gactggaagc
     5341 cttattatca gaccgaagaa gagatgattg cgcatttttt agatgttatg tcgccaatgg
     5401 tagatgctgg aaagatgttt tgctttttag tccaattccc ctcatcattc aaatgtacga
     5461 aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt tgagggttat cctgtttcta
     5521 tagaattgcg ggattattct tggtacagcg atgaatttct tgaacgtaca agaaacttta
     5581 tgtctagagc cgctttttct ttagccatca tcgatgagcc acagttgctg aatacaacga
     5641 ttccttttga tccatttgtc accaatccga actttgctct ttttcgattc catggtagga
     5701 atgcgcaagg atggcaagca aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt
     5761 attcagaaaa agaactgcaa gaactagcac atgaagtcaa agtagttgat tctaagacaa
     5821 aagaggtagg tgtgattttt aataataatt ctggtggtga tgcggctgaa aatgcattag
     5881 cactgcaaaa aatattaaat atcaagccag atggactcaa tccaacccaa atggatctgt
     5941 tttaagggct tattttattc aagaaagggg cgaagtctat gatcaaagct attttttttg
     6001 atattgacgg aacattggtc accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa
     6061 ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc aacggggcgc agcccagtaa
     6121 agatcaaaga aatcattgat gaattggaat tagatatgta tgtcgtgtat aatggtcaac
     6181 tggtttttac tgcagatcgc acgattatcg accacccttt tgaacaaaaa gtattggaac
     6241 atattgttga atttgcagat gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt
     6301 tagatggatc aacgacgatg ctattaggac aatcgatttt tatcaagcga ctagtcagct
     6361 ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat cctccaggtt tttagccctc
     6421 accgacaaaa ggatcgttat gaaacgttag agatattaaa agaaccaatt tatcaatgta
     6481 tattgctcag tccggagtca gaacagaaaa agttagaaca tcgctttccc gagtgtacgt
     6541 ttcagcgttc aaatacgtat actgtagata tcatcccaaa aggtggttca aaattactag
     6601 gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg
     6661 atcattacaa tgatatcgag atgctgaaag gtgtaggaat tggagtggcc atggggaatg
     6721 ctcagatcga agtaaaacat gctgcagact ttgtgactca atcaaatgaa gaagatggga
     6781 tatacgaagg actgaaacac ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc
     6841 agtatggaag cagagacacc ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca
     6901 agaagaccag agatacccac ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc
     6961 aaaatagaag aacttgttga atttgttgca gctacttcac ctaataaaga acaactgcat
     7021 gaagctgtta tgctgctgca cgaagcatta gataaagcag aagaaaaaat acttggtaaa
     7081 acacagtggg gaagtgatcc gctggttgaa caagtagatg cactgacaga tatcctttac
     7141 tttacttatg gttctttttc attactagga atagtgcctg atcgaatatt cgaaatcgtc
     7201 catcaggcaa atatggggaa attatttcca gatggaaaac ctcattatga ccctgtaaca
     7261 aataaagtgt tgaaacctga aaactggcag atggaatatg ctccagaacc taaaatcgcg
     7321 gcagaaatcc aacgtcagat tcaagaagcg attgctaaga agaaatccgc tgatataggc
     7381 atgtgcacaa atgaaaaaga gtgatcagag tttcactatt tttgttatct ttatacgatg
     7441 gaaaaacaaa agattttcgt ttaagttagt gaatgcatga aaatagtatg ctataatcta
     7501 gaatagatgt cttttgcacg agagacgtat tatttggatt tatgccaagc agaacgccat
     7561 ctgttgcata aaagatgcat actgaaaacg gaggaaccat aaatgggaaa taatgttatc
     7621 atcggaatcg tcattgcaat cattatagtc gccattgtcg tctttttggt tagttacttg
     7681 atgaagaaaa aaaaccaaga tcgattggat gccttagaaa aacgcaagga agaattattt
     7741 gacttgccag tgattgaaga agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc
     7801 caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct
     7861 gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa
     7921 ggaaaaaaag cagttgttga agcagaagct acaatggacc aaatggagaa acaagtgacg
     7981 gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag aacgcaactc actagctgtt
     8041 cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa
     8101 tacggaccag ctctttttga attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg
     8161 cagtttgtga ctttaaacac ttctggagac cctgttgaag cccgcgaggt tttggataaa
     8221 gcagaaaaac atacgtatga agtggaagct ctgatgaaaa aaattccacc gctgtatgag
     8281 gagctccata ccactttccc tgaacaactt gaagaaatca gtgatactta cgagaaattg
     8341 atggaagaac aatatgtatt cccagaagaa gatcttgctg aagatatcgc aaaagtaagt
     8401 cgaagaattg aaaattcatt agccaatctt gaaaaaacag aagttgaaac ggtcgaattt
     8461 gaaaaccgtg aaacagctga tttgattgat tcgttatatg atatcttaga acgggaaatg
     8521 gaagctcaga gatatgtaaa aactaaccaa tcaacgattg cagagtatat caaacataca
     8581 acaaagaata accgtcaatt attgattgag ctggatcaca catctcaaag ctatacattg
     8641 aaccacaatg aaattggccg tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt
     8701 caaaatgaac agatgattcc acagatccgt caacacgaaa ttccttattc agaagtgcgc
     8761 acgttctata aaacagtctt caaagtctta gaagatatcg aaaagcaaca agtagagatc
     8821 gacgactcat tacatgaatt gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca
     8881 ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg
     8941 ccgaatgatt acctagaatt cttttttgtt gctactgacc gtattgaaga actaagtgtt
     9001 gtcttgaata aaattcgagt aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa
     9061 gaagatctag aattattaga taagaagaca catgatttag tagatgcagc agcattaacg
     9121 gagcaaatgc tccaatatgc aaatcgttat cgtcatacac acgaagaagt gagagaagca
     9181 attgataaaa gctactattt attcaataaa gaatatcact atcaagaagc attagacgaa
     9241 atcggtacag cattagagcg agtagaacct ggtgctttca aacgcatcga agatttttat
     9301 tttaatcatc cagacttagt ataaagcaaa aaagttagta aaccagcaag agcaagacgg
     9361 attattgtct tgttcttgtc tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc
     9421 acctgctctt gaaagcacct tggacaaccg ttataataga aaagaatttc aaaaaagagg
     9481 ggtttcttat gatatatttt gataacagtg caacaacccc aatctttccg caaagcttag
     9541 atgcatatgt aaaaaccagt cagcggatta ttgggaatcc atcaagcttg catgatttag
     9601 gcaatcaagc taaccggttg ttgcagcaag caagaaagca gatcgctgat ttgatccatg
     9661 tagaaccaga agaaatctat tttacttcag gcggaacaga aggagataac tgggtattaa
     9721 aaggaacgat gatcgaaaaa agagaatatg ggaatcatat gatcatttca ggagtcgaac
     9781 atccggccgt cagcgagaca gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg
     9841 ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag
     9901 agacagtatt agtctctgtt atggctgtga ataatgaagt aggatcgatt cagcccatcc
     9961 aggaaatcag tgatcttttg gatgattttc ctaaaattca tttccatgta gatgctgttc
    10021 aggcgattgg aaaagtacca ttctcgcaat ggttgacaga aagagttgat tttgcaacat
    10081 tttcagctca taaattccat ggcccacggg gaactggttt catttattgg aaaaagggtc
    10141 gtcgtttagc accattactg acaggtggag ggcaagaaag aaatcagcga agtgggacag
    10201 aaaatgttcc aggtattgtg gctatggtta aagctttacg tttatcatta gagataaagg
    10261 aacaaaaacc tgagcagaca caacgcttaa aagtagctct aatcaatgcg ttgaagacgt
    10321 atgagaaagt gacgattttt tcagaaggaa ctgactatgc accgcatatt ttatgttttg
    10381 ctctaaaagg aattcgcggc gaagttctcg tccatgcctt agaagaaaaa caaatatttg
    10441 tttctactac tagtgcctgc tcctcacgta aacatatggc aagcagtaca ttgcacgcaa
    10501 tgcatgtacc aaatgatctg gctacatctg ctgttcgaat cagcttagat gaaaacaatt
    10561 cgcttgcaga agtcgagcaa tttatgatta ttttcaagca gctttataag aaattttctg
    10621 caatcaatta aaagaggagt gttcttgtga aatacagtga aattatggtc cgttatgggg
    10681 aactttcgac aaaaggaaag aatcgaaaaa gctttatcat gcaactagca caaaatgtca
    10741 gacaagcttt gaaagacttt ccagaaatca aaatccatgc tgatcgggac cggatgcatc
    10801 ttttattgaa tggcgcagac gggcagctag ttattccaaa actagcaaaa atatttggga
    10861 tccaaaattt ctctccaagt attcgtgtag aaaaagatgt cgaagttttg aaacaagctg
    10921 ttcaagacat catgaaagaa atttatactg ggaaagaaac atttaaaatt atcgctaaaa
    10981 gaagcgatca ccaatttgaa ttgacaagta atgaattgaa tcaaacatta ggtaatgctg
    11041 tttttgatat tttcccgcat atccaagttc aaatgcgtca accagatatt ccattacgtg
    11101 tagagattcg gcgtgatggc gcttatctat cgtatgaaac gatcaaggga gctggaggat
    11161 tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc tggggggatt gattctcctg
    11221 tagcagggta ctttgccatg aagcggggag tggaaattga agctgttcat tttgctagtc
    11281 cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga tctaacagcc aaactagcac
    11341 cttatgtggg aacgatccat tttatcgaag tgccatttac cgagattcaa gaagaaatca
    11401 aaaagtcagt gccacaagga tattggatga caattactcg tcgaatgatg ttgcgtttga
    11461 ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc
    11521 aagtggcttc acaaactttg caaagtatgg tggctatcaa tgaagtaacg aatacgccaa
    11581 tcattcggcc tgtagcgaca atggataaac ttgaaatcat tgaagtagca gaacagatcg
    11641 atacatttaa tctttctatc cagccttttg aagattgctg tacgattttt gctcctccac
    11701 agccaaaaac acgccctcaa ctggaaaagg tccatcaata tgaagagcgt ttagcaatag
    11761 atgaaatgat ccaacgagca ctagctggct tgaagatgga agagatacaa gcagaatcgt
    11821 ctaatgaaaa aactgaagaa ttttcagaat tgctataaga ggttatgaaa atagtgtttt
    11881 gaccagagta ttagagaaac aaaacgaaaa aatagctcca tgtattttac cgtttttgaa
    11941 ctaatgtcga agattagctg attgaatacc atttatcaag aagttatgga aaagccggtt
    12001 cttttttact aactgaaaaa taaaatttta tttagagatc aagctacagt tgataaatga
    12061 tataaaaaag agaatttccc atgaaatagc gaggttgtga cagaagtggg cagcttcaag
    12121 aaataagaag gaatttacga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt
    12181 tcgaagaagt tacttctgga acaccgtgta tcagaggttg cgaaaaagct gccctgcatc
    12241 aagtaataag aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat
    12301 taccgcagat gcaagctttt gaacaccgtt ttatttgtct cacacccttt tccttcctac
    12361 ttgtaacctt gctcccgtgt tagaaacatg ttacactaaa tatgtgaaat tattaacaag
    12421 gagcgtcgtt atgcgagaaa aaacagatac actgccaaaa gcaactgcga aaagattacc
    12481 tttatattta cgctacttga agatgctgga tgactcaggt ataagtagaa tcaaatcaaa
    12541 tgagttcagc gaaatcaccc aaattccttc tgcaacgatc cgcagagact tctcgcaatt
    12601 aggcgagcta ggcagaagcg gatatggcta tgatgtccct tttcttatcg atgtattcaa
    12661 taatatcctt aatactaaag aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg
    12721 caaagcattg aaacacaaca attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt
    12781 tgataatgat ccagcactga tcaatcgagt aattgatgga gaaatgatct atccgattga
    12841 tcgtttcgcg gagatagcca aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag
    12901 taaatattca caatctgcta ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa
    12961 ttttgcgcct gacagagtaa ctgtcccagc ttatgtcaat gttcaataca ttgatttgac
    13021 aaccgagttg cagacgctta tttactttga tgagaattac tcagaagttt tttcttagga
    13081 aaatttgagt aggcttattg atcttgcgag aactcgtcgt aattctttca aaaatttttt
    13141 agtactaaat gaagtggttt tttcacgacc acttgttgac taaaaaaaaa aaccattgta
    13201 gaatgaagca taacgctgaa caagaggagt aactggcaca ttcattctta gagagagaac
    13261 tatctgctga aaggttctca atgacaacca gtgaaggtag cttgggagtt tctgtcttga
    13321 atggagtaga gatagacgag tcgtgatcgt taactcactt gagaggcaat aatgattgat
    13381 tattgcaatt taggtggtac cgcgtaaatc atacgtccta gaatacgaaa gtattttagg
    13441 acgtttttta gtttaaagga ggaaaacagc atgacggaag aaaataacct atcaacaaaa
    13501 tataatccgc aagaagttga agctggacgt tatcagcatt ggttagaaca agatttgttt
    13561 aaaccgagcg gagataaaaa agcaaaacct tattcaatcg taatcccgcc cccaaatgtt
    13621 actggaaaac ttcatttagg gcatgcttgg gatacgacct tgcaagatat gctgattcgt
    13681 caaaagagaa tgcaaggata tgatacttta tggctgcctg gtatggacca tgcaggaatc
    13741 gcgacacaag caaaagtaga agcgaagctt agagagcaag ggatttctcg ttatgatcta
    13801 ggtcgtgaaa agttcatcga tcaagtgtgg gaatggaaag aagagtatgc gagccacatc
    13861 cgcgaacagt gggctaaaat gggcttatct ttagattaca gtagggaacg ttttacatta
    13921 gatgatggat tatctgaagc agtacgtaaa gtttttgtta ccttatacga aaaaggtttg
    13981 atttatcgtg gtgaatatat catcaactgg gatcctcaag cacgaacagc tctatctgat
    14041 atcgaagtta ttcataaaga tatcgaaggc gccttttatc atatgagcta tgaactagca
    14101 gatggatcag gcgtagtaga aattgcgaca acccgtccag aaacgatgtt gggagatacg
    14161 gcgattgctg ttcatccaga agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt
    14221 ctacctttag tcaataaaca aatcccaatc attgcagatg aatatgtaga tatggaattc
    14281 gggacaggtg tagtaaaaat cactccagca catgatccaa acgactttga ggtaggtaat
    14341 cgtcatgatc ttccacgcgt aaatgttatg gatgatgatg gtacgatgaa tgaactagca
    14401 ggaaaatatg ctggaatgga acgttttgct gctcgtaaag caatcgtgca agatctgaaa
    14461 gaaatcggtc gattgattaa aatcgatacg atgatccaca gtgtgggtca ttccgagcgg
    14521 acaggtgtcg ttgttgaacc tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta
    14581 gcgaaaaaag ccattgataa ccaagaaaca gatcaagcag tggaattctt ccctccaaga
    14641 ttcaatcaaa cgtttttacg ttggatggaa aatgtccatg attgggtaat ctcacgacaa
    14701 ttatggtggg gacatcaaat tcctgcatgg tatcacaaag aaacaggtga aatgtatgta
    14761 gggatggaag caccagcgga tagtgaaaac tgggtacaag atcctgatgt tttagacact
    14821 tggtttagtt cagctttgtg gcctttctca acaatgggct ggccagatac ggaaagcgaa
    14881 gattataagc gatacttccc tactagcact ttagtaaccg gttatgacat cattttcttc
    14941 tgggtaagca gaatgatttt ccaaagcttg gaatttactg gcgaacgtcc atttgagaat
    15001 gtcttgattc atggattgat tcgtgacgaa caagggcgga agatgagtaa atcattaggt
    15061 aatggaatcg atccgatgga agtaatcgaa aagtatggag cagatgctct ccgctggttc
    15121 ttatcgaatg gttctgcacc aggacaagat gtccgcttca gctatgaaaa aatggatgct
    15181 tcttggaact ttatcaacaa aatctggaat gcttcacgtt tcgttatcat gaatatcgaa
    15241 ggaatgaccg tagaagagat cgatttatct ggagaaaaat cagtggctga ccggtggatc
    15301 cttactcgtt tgaatgaaac gatcgaacga gtaactgagc tatttgatcg ttttgaattt
    15361 ggtgaagcag gtcgacaact gtataacttt atctgggatg atttttgtga ctggtacatc
    15421 gaaatgagta aagaagtact ttatggagat gatgctgttt caaaacaaac cacccgtagc
    15481 atcttagtcc atacacttga tcagatccta cgtttattgc atccaatcat gccattcgtt
    15541 acagaagaaa tatgggaaca tattcctcat cagggaaatt ctctagtagt ggcagaatat
    15601 cctgttgtac gtccagagtt tgatgatgaa acagcttcta aagggatgga agtattgaaa
    15661 gaattgattc gagccgttcg aaatattcgt tcagaagtga atacaccatt gtctaaacca
    15721 attacgttgc taattaaaac aaacgatgct aaaatcgatc aatttttagt tgaaaataca
    15781 agctatatcg aacgcttctg taatcccgaa gagttgacga tatcaagtga gatcactgcg
    15841 ccagatctag cgatgtcagc tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga
    15901 ttgatcaata ttgaagaaga aatcaaacga ttagaaaaag aactggaaaa gtggacaagc
    15961 gaagtaaaac gtgtccaagg aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa
    16021 gaagtagtag aggcagagcg tgcaaaagaa aaagattatt tagagaagca aacggctgtc
    16081 aaagaacgaa tcgaaagttt gcgtactatt caataagaat agaaaaagag agtgtgaaat
    16141 cagctagcta ttactaaaag ctagctgatt gaacatcgtt acaggaaaga gtttgagcct
    16201 cactcttagc ttcgattcat aaggagatgt gacataaatt ttgtcgcatc tcttttttag
    16261 tctactctta ttatgtagga gaaggaggga ggtggatcat gtatcaagag tcaataaagt
    16321 agttgataaa atcgttcctc tctctgtttc ttttctcttt gtcatacttt tatttttctt
    16381 atgtgttcta tttgttataa taaaagagaa atgggggaat tgttgatgac gattgaagaa
    16441 gcaatagaat ggatccatag tcgattacca tttggttcac gtcctggcct tgaccgagtc
    16501 gaagctttat tagaagtggt agggcatcca gagagaaaag taaaaacgat ccatatcgct
    16561 ggaacaaacg gaaaaggttc tactgtagct tatttgagaa gcctattaga agaggctggg
    16621 ctgacagtag gtacatttac ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc
    16681 aatggcaaaa gcattccaga caatgatttg atccagcttg taaaaaaata tcagccactg
    16741 atcaaacaac ttgatcaatt tgaagcagta gctggtatta ctgaattcga gacattaaca
    16801 gcaatggcat ttgattattt tttgatgaag aaagtggacg tagcgattat cgaagttggc
    16861 ttaggcggct tattggattc taccaatgta gctgtaccaa tgctgacggg aataacaacg
    16921 attggacttg atcataccga aatacttgga gatacaattg aagaaatcgc tgcacaaaaa
    16981 gctggaatca tcaaacaagg cgttccagta gtaacaggaa atattgcccc agaagctttt
    17041 actgtcatcc aacaaacagc taaagcaaaa aatgcaccgc tttatgtata cggggaaaat
    17101 tataaaataa actatctgca tcctgatgag atgtggggag aagtatttga attttttaat
    17161 gaaataggaa agatccctca attaaccact ccgcttttag ggcaacatca agctgaaaat
    17221 gcaggagtag ccatcgaatt gtattttgtt tactgtcaat tggaaaagct gcctttccga
    17281 gaaaaagatg ttttgaaggg attgaagaaa tctcgatggc caggaagaat ggagcgtttg
    17341 agtaacgagc cattagttgt tttagatggt gcccataata cgcatgcaat gaaccggctt
    17401 gttgagaatt tgaaaaaaga attcaaagga tatcaaatca acattttgtt ttccgcctta
    17461 cgaactaaaa atgttagaga aatgctagaa cgattgcagc aagtaccaaa tgtccatatt
    17521 tatttgacga cctttgaaca tccaaaagcc atcgaattaa gtgaagtatc agatttagca
    17581 agtgagagag tatcaatcgt ttcattgtgg caattcggat tagcagatat attggaaaag
    17641 atgacaaatg aagacgtatt agttgtgacc ggttcacttt atttcatttc agaagttcgc
    17701 catttattaa tacaattagg aggagatcat gaagtataat gcagtgattt ttgacatgga
    17761 tggtttgctg ttcgacacag agttagtgta ttacgaagcc acacaagtag tagcagacaa
    17821 gatgggtttt ccttatgata aagacctgta tttaaaattc ttaggtgttt cagatgaaga
    17881 agtatgggcg aattatcata tgattttctc ggaatttggc aaaacaagtg tacaaagatt
    17941 catagatgat tcataccagg aaacgcttga acgtttttcg caaggagcgg tacaaatgaa
    18001 gcccggtgtg ttggaactcc tctctttttt agaagaacat cagattccca aagttgtcgc
    18061 ttccagtaac caacgagcag tcattgaact tttattgaag aagaacaatc tgtttgaacg
    18121 atttgagacg attgtttctg cagaaaatgt tcaacgagca aaaccagacc cagaaatttt
    18181 cttgttagct aatcagaagc taggtacagc gaagaaagaa acattgattt tagaagattc
    18241 acaaaatggt attttagcag cttctgcggc tgagatcccg gtagtcatgg taccagatct
    18301 gcttccacca tcccaagaat tgatcaaaaa aacagctggg gttgtttctt ctttacatga
    18361 aatccctgca ttgttaaaat aaaaatccat ctcttttcgt atcttatttt agataagata
    18421 gaaaggagat gtttttttgg gaaaacgatt gatcaaagaa gtacctacta gctctttacc
    18481 aagggaacgt atggaaattt atggcgcgga agctttatcg gatcaggagc ttttggcaat
    18541 cttactgcgt accggtcaac acccctatag tgtgatgtcg attgctggaa atctgttaaa
    18601 aacgtttgga ggactggcct ctttacgaca ggctactttg catgagttag aagagattcg
    18661 agggatagga cgcgtgaaag caatcgaaat caaagccttg atcgaattag gtcgacgaat
    18721 tcagacggat catgaaagag cggctccacc ggttcgtacc agctacgaat tggcccagca
    18781 attgattctg gaaatgaaag atcacaaaca agaacatttg gtttgtattt atttggacac
    18841 gaagaatcaa gtgatcttaa aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc
    18901 tcatcctaga gaaattttcc attatgctgt acgttattgt gcagcaagga tcgttttagc
    18961 gcataatcat ccttccggaa tatataagtt agtacacata aattatataa ggaaatttac
    19021 tgttatattg ccatctggaa atactattat gttatcgata attgaacgcc ataatgcatt
    19081 tttttcttct tccgaagatt ctttataa
//
LOCUS       DAJSNV010000051        19101 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSNV010000051 DAJSNV010000000
VERSION     DAJSNV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.052"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            27..581
                     /locus_tag="ON516_001709"
     CDS             27..581
                     /locus_tag="ON516_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345194.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(754..1227)
                     /locus_tag="ON516_001710"
     CDS             complement(754..1227)
                     /locus_tag="ON516_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345195.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1239..1775
                     /locus_tag="ON516_001711"
     CDS             1239..1775
                     /locus_tag="ON516_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3345196.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1815..2186
                     /locus_tag="ON516_001712"
     CDS             1815..2186
                     /locus_tag="ON516_001712"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3345197.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2470..3741
                     /locus_tag="ON516_001713"
     CDS             2470..3741
                     /locus_tag="ON516_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3345198.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3986..5011
                     /locus_tag="ON516_001714"
     CDS             3986..5011
                     /locus_tag="ON516_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3345199.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5305..6414
                     /locus_tag="ON516_001715"
     CDS             5305..6414
                     /locus_tag="ON516_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3345200.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6497..7393
                     /locus_tag="ON516_001716"
                     /pseudo
     CDS             6497..7393
                     /locus_tag="ON516_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7409..8233
                     /locus_tag="ON516_001717"
     CDS             7409..8233
                     /locus_tag="ON516_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3345201.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8265..9575
                     /locus_tag="ON516_001718"
     CDS             8265..9575
                     /locus_tag="ON516_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR3345202.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9723..10982
                     /locus_tag="ON516_001719"
     CDS             9723..10982
                     /locus_tag="ON516_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3345203.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10992..11864
                     /locus_tag="ON516_001720"
     CDS             10992..11864
                     /locus_tag="ON516_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3345204.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11876..12706
                     /locus_tag="ON516_001721"
     CDS             11876..12706
                     /locus_tag="ON516_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3345205.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12746..14929
                     /locus_tag="ON516_001722"
     CDS             12746..14929
                     /locus_tag="ON516_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3345206.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14990..16210
                     /locus_tag="ON516_001723"
     CDS             14990..16210
                     /locus_tag="ON516_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR3345207.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16207..17667
                     /gene="gtfA"
                     /locus_tag="ON516_001724"
     CDS             16207..17667
                     /gene="gtfA"
                     /locus_tag="ON516_001724"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR3345208.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17744..18850
                     /locus_tag="ON516_001725"
                     /pseudo
     CDS             17744..18850
                     /locus_tag="ON516_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6487 a         2909 c         3725 g         5980 t
ORIGIN      
        1 gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta aggacaaatg
       61 aagagataca agattttcag ttttggctaa gaagaacaag taatccggaa agagatagtt
      121 ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata ggattaaaag
      181 ttggagatat acgaggcaaa agtaagccta ttattgttga acataaaaca ggtaagcgaa
      241 aaccgatttt tatagataat ttgagagaag aaattttatt atatactgaa ggtaaagaag
      301 aaaatgattg gttatttccg agtcggcaac aaggtcgaca tataacaagg gatcgtgtct
      361 atcagatata tgcagatata gcagaaaaat taggacgaga tgatatagga acacatacat
      421 taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct accttaatgt
      481 ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata actgaagatg
      541 aaataggagc atcacttcgt ggatttaaac taggtgtata atttaaaaat ttttataaac
      601 agtaaagaca aagattaaaa gctattttta tctagaacga ttttatttca taaagttcgc
      661 ggaataaaag ctggggttac tggtacttgg tataggttcg tgttatataa actaagtaat
      721 taaataacat ttttaggttg gaaaaggatg aaattaaaaa aagagaatca atacggtctc
      781 aaaaacgcaa acgctgcgga ataagaaaaa tactgataac tcaacgttta gcagactatc
      841 taatagggat agtctgcttt tttcgttttt gaccatactt ttgaccttct gcaaacttcc
      901 ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga attgccgttt cctctgtctg
      961 ctttttcgcg atatgggtgt atatatcatc catagtggtt ttaatatctg tatgtccaag
     1021 tcgaacatac acttctttga cagacgcacc agattcaaag agcagactac aatgggtatg
     1081 acgaaaacca tgaagtgtga tatgttttaa gttattctct ttaatgatga ttttcaaatt
     1141 gtgatttaca tagtctaaat aaaggggctt attttcttct gatgaaaaca aaagttgttc
     1201 accatctttt ataggtttga ttctcatttt tagatattat gcccgcacgt attcatcaca
     1261 tttctattgt gaatcgcttt attcgaccga catttgactt ttatcataat atactcggtt
     1321 taaaattact aatgaaaaca attaatcaag acgatcatac catgtatcac ctgttttttt
     1381 cagataatca ccaccgtgtg ggaactgaac tgactttttt tgaagtacaa gaagggaaca
     1441 accagttttt tggaaccaat acgattgaac gaacgatact aaaagttcct tccgaagcat
     1501 cattgcattt ttgggaaata tattttgaaa cacaaggcgt ttgccattat ggaatcgaat
     1561 cattttctgg acgacctatc ttacgttttg aagggccaga tgcaacacga ttagctttag
     1621 ttcccttacg cgaatttgaa gatattgatg attatttccc gtatgtcaca gaccagatcc
     1681 ctacagagca tgccatttta ggaattgatg ccattcaact acgcgtacaa tattccgatg
     1741 cgactgaacg agagttattg tcggtaggct ggtagcacaa agaatacgtc agtttctttt
     1801 cgggtcatca acgagtgaca atcttagaaa atcaagacac tcagttctat caagaagttc
     1861 agattattca agatcgtgag aatccagttg ccattgaagg gattggtagc gtccatcatg
     1921 ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat cgacaaacaa ttacaagagc
     1981 gcaattttat aaattcagga ataaaagatc gtgaattttt tgtatcactt tattatcgtg
     2041 acccaaatca attattgatt gaaattgcta caggggaagg aaacctagat gcaaaagcgt
     2101 atgaaaatca aagtccacgc tttgaagaaa ttcctttatt tttaccgcag tacttaaatt
     2161 tcattcgtga acaagtggag caataattag ctaatcaaat ccatgacgaa gagtaagagg
     2221 ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat ccattctaaa gaatgttgat
     2281 ttgatgcggt tcctattttt ataaactatc attttcgact aataaaacta cttatgagtc
     2341 agcctctctt tttttcttga tttttatacc tctttctagg taaactaaac atctgccaaa
     2401 aataccgtgt ctcattgaaa cattctcaac tatttcaaaa taaaggggag taccaaatga
     2461 acgagtatat tgaaattatc cacgcaagag aaacaactta aaaatgtcag tctcaaaatt
     2521 cccaaaaata aaattagtat ttttaccagc gtatctggtt caggaaaatc atcgattgtt
     2581 tttgatacta ttacccaaga agctggtcgt caactgaatg acacctatga tagttttacc
     2641 cgtttatttt tacctaaata cagtcgtcca gaggttgatg agattgataa tctttctacg
     2701 gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc gctcaacgtt agggacgatt
     2761 actaacatca atgcgttatt tcgtgtgttg ttttcatgtt ttgcaacacc tagtttagga
     2821 tatgcaaatg cttattcatt taatgatcct agtggaatgt gttctgtctg tcagggaatc
     2881 ggtaagacga ttgcattttt aataggattg ctttattttt cggctattga taatgctgat
     2941 tttgtatttt ctgatccatt taccgcagaa gttttgtctg gaattgagga acaggcaaag
     3001 aatcatggtt attttgtatt ggtgcataat gttaattcag cagaagatgt taaaactatt
     3061 cagagaaatt ggcgttttga ggggttcatt gttgttggtg tacgtgtaaa agaatttttg
     3121 gaattaaata acgtttttga tggtcctgtt gtttatattg atacgcatct ttcaaaagag
     3181 acattaagag aacttgaaca acaaatgaat cgtttatttg taaatacaaa tgattaccaa
     3241 gctagtacat tggcagtgga acatttgatt cattatggtc atgagaaaat agcgtttctt
     3301 tcttttgact acattgtagg tgaaccaagt gttattcaag aacgacatca agcttatatc
     3361 gatacattaa agaaatatca tttaaccttt aaatcgcatt ggatatataa caactctgaa
     3421 tttgagcgaa tttatcaaga attagctgaa tttactgcaa ttgttgttac tggagattat
     3481 ttagcggcta aattagtaaa atttttaaaa aataagaata aacgagtgat ggaacaatta
     3541 tcgattattg gatttgatga tattactttt gcagaattaa tggatcctgc gttgacaaca
     3601 atacgactaa atccaggtaa caagggggcg attgcttttc accaactgat tggactggcc
     3661 aaacaagaag aaataaaaaa tcaagtacta ctattagagg gagaattgat ttctcgagac
     3721 tctgttcaca aaatcaaata aaaaagctct gtgaaataag tatagttaac aataaaatat
     3781 ccgaacgata gagagaatga actgtgatct agcagttcct cgtcgcaaat tcactagaat
     3841 tagcattatt tactctaaat tgttcggata ttttttcttg tttttaatta tgattacagt
     3901 gcctattttt aacgaaattt gcttattttc aaaatgtatg attatatgtt agtaaacact
     3961 ttcaaatatg aaaagaggag ctattatggt aggaattagg gatgttgcaa aacgggcggg
     4021 tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat ccgacacttt cagtaaccga
     4081 tgaaaccaaa cgaaagattg cagaatcggt taatttttat cattatgtca aaaaaagtcc
     4141 cgttgcaaaa aatttaacaa atattgcatt aataacgaca gtttctgaag tagatgagtt
     4201 ggaagatccc tattttcgaa cgattcgcag agggattcaa gtcgaagcag aacaaagcaa
     4261 agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg ttggagcaag acgatttgct
     4321 aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct tctgtgattg aagaagtatc
     4381 agcaattaat tcgaatgtcg tagtgattga tgaccctagt attgaagctg aagtagatgc
     4441 agtttatact gatttaacaa aagcaacgaa tcagcattta gatcgattgt ataaaaaagg
     4501 acaccgaaac attgcgttta tcggaggagg acgtgttcgt ttagatagcc aaggaaataa
     4561 acacgtgtca gatgatgatt atcgaaaaat agcctatgag acgtggatga aacaaaaagg
     4621 attagaaaaa tatagtcaaa tttatttaga aggctggacc acacttgacg gaatgcaggc
     4681 agcagattta cttttgaaaa aacatgctaa agataagttg ccgactgcta tcatggtagg
     4741 aaatgatcct attgctgtgg gggtttatcg ttcacttcaa aaaaatggtc tacatattcc
     4801 agaagatatt tcgattgtga gttttgataa tattgaagtt gctgaatttt taacacctgc
     4861 attaaatacg gtgaatgtga atacagaaga aattggaaaa ctagcagttc gcatggttaa
     4921 tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg attgtttcta ataatattat
     4981 tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa catccaaaaa agggtgttta
     5041 cttttttgtt tactaatatt tttagtaaaa aatctcgaca aaacattaag taaacagtta
     5101 tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag gaattaagat gaaacatttc
     5161 aaaaaaatag ctttaacagg gatagctgtt ttaactggga cattactcgc agcatgtggt
     5221 gggggagata gtaattctaa agacgggcaa gtaactttaa gctatgcgag ttgagatact
     5281 gaccaagctc caggactaag aaaaatgtta gatgaatttg aaaaacaaaa tcctgatatc
     5341 aaagtagaaa tggagacaac gccttgggat caatattggg ttaaattaga agctgcaaca
     5401 acgggaggta atatgccaga tgtagtaacc atgcactcta gcgaatccta taaatatatg
     5461 tcacagaatg ctttgatggg acttgatgat gttatcaaag aaaatgatat tgacatggga
     5521 aactttactg aaggaattgc tgatttctat acgcttgaag acgaactata cgcaatccct
     5581 aaagatgctt ctgtagtggg gctatggtac aacaaagaat tatttgataa tgcaggtgtc
     5641 gattatccag atgaaacatg gacatgggaa acactcagac aagctgcaat tgatttaaca
     5701 gatgaatcaa aaggcatcta tggttttgca tcacatattg gaacagagac tggctactgg
     5761 ccatttattt atcaaaatgg gggagaagta tttgccgatg acaatacaaa atcaggtttg
     5821 aataccccag aagcacaaga agctttagct ttttatcgag atattgcact tgttgatcat
     5881 gcttcaccaa caattacaga attacaagat cctgataagt taagtcgttt tcaatcaggt
     5941 agattagcaa tgatgattga aggaaactgg tttgcaccgg gatttttgaa aaatgactat
     6001 ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa aagttaatgc aacaattaca
     6061 aatggacttg gttgggctgc cagtgcgagt acaaaatacc cagaagaagt gaaaaaacta
     6121 atggcatttt tagctagtga ggacgccaat aaaatccaag cggaaacggg ggcatcaatc
     6181 ccagcactaa aaggctttga agacgagtgg gctgcttctt cagatacaat tgatttgagt
     6241 gcttttggaa aaatgttgga gtatggagtc cctcgtccat acaataaaga tggattacga
     6301 gctgaacaag cagaagttca aattattaac cgtattctgt ctggtgaagt aactgttgaa
     6361 gatggaaccg ctcaaattga aaaagaagtc aatgcgatat tgagcggaaa ttaaattagt
     6421 taattataca atatacaggc gaaaggattc tatttcgtct gtatattaat ggatgaatac
     6481 caaaaagggg gatattatga aaaatcgaat ttttggaaaa tatcgtaaag atcaagtgat
     6541 atggggctat atttttattt ttccaacggc attaggatta attttattaa atatatggcc
     6601 agcaattcaa acagcaattt taagttttca aaaaacggta gggttcggtg atactgaatg
     6661 ggtcggatta agaaattacc aaaaaatgtt tgcagacggt gaagtttttc agtctttatt
     6721 aaacacatta atttatgcag tggtttcgat acctagtatt gttgtattat ccttgttggt
     6781 ggcagtttta atgaatagaa aagttaaagg attgtcgatt tatcggacaa tttattttct
     6841 accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg cgctggatgt tcaataatga
     6901 ttatggactg attaataaca tgttggctaa aataggtctc gatggaccag catggttaac
     6961 ggatccatcg attgcgattt attctattat cattgtagga gtttggagtg ctattggtta
     7021 taacatggtg ttattactag ccggcttata agaaattcct aaagattatt atgaagcagc
     7081 ggaaattgat ggtgctagtc caatccagca gtttttcaac attacgttac cattagttac
     7141 accaacatta ttttttgtag ttgtaacaac agttatcaat gctttccaag tatttgatgt
     7201 tatttttatg atgattactc ctaatagtac cgccatgttt aaatcacaat cattagccta
     7261 tttattctat aaacattcat ttattttaaa tgacaaggga tatggttcgg caatcgttat
     7321 gttcctttta gtgattatat taattattac tgcaattcaa atgaagttgc agaaaaagtg
     7381 ggtaaactat taaggaggct atcaaattat gaaaaataaa tttttttcaa atgcgccaat
     7441 ccatatcatg cttattttag gtggtattgc aatggcatta ccttttattt ggatgatttt
     7501 aacttcaggg aaaacgttgg ttgaatctac ccaaattcca cctacaattt tccctgagaa
     7561 atttcagcta agtaactatc aacaagtact tgatttatta ccattcatgg ctttttatat
     7621 caatacattt ttaatggttg ttggacgagt gattggctcg gttttatttt cagcaatggc
     7681 tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat atccttttca gcttagtact
     7741 tattcaaatg atgattcccg gttccatttt tattactcca cagtatttat tagcacaaaa
     7801 attgggtatt ttaaatacaa ttccagcatt aatattacca ggggtagtta gtgcgtttgg
     7861 tacgttttta ttaagacaat ttttcttagg tttacctgat gaattggagg aagcggccta
     7921 tttagaaggt gccaatattt ggacgatctt tttcaaaatt atgttgccgc ttgctaaatc
     7981 aggattggtt gcacttggaa tattcacagc gttgtttgca tttaaagatt taatgtggcc
     8041 attgattgtc aatacttcaa ttgataagat gcctttagct ccgggattag caaacttaca
     8101 aggacaatat gccacgaact tcccacaatt aatggcaggt tcgttattag caatttggcc
     8161 aatagttgtg atattcatcg ttttccaaag aaaattcatt gaaggaattg ctacaacagg
     8221 tagtaaatta taaaactatt aattagtgat tggagagtaa gtacatgaca aaaattacat
     8281 ttattggtgc aggaagtact atttttgcaa aaaacgtatt aggcgatgcg atgttaactc
     8341 caagtctaca agatgcacat attgcattgt acgatattga tgaaaaacga ctaaaagaat
     8401 caaatttaat gcttaaaaat attaataaaa attcgaatga aaatcgtgct gatattgttt
     8461 cttattatga tcgcaaagaa gcgttaagag aggctgattt tgttatcaat gctattcaag
     8521 tgggtggtta taaaccttca acagtgattg attttgatat tcctaaaaaa tatgggttgc
     8581 gacaaactat tggtgacacc atggggattg gtgggatttt tagaacccta cggacactac
     8641 cagtaatgtt agactttgct aaagaaatgg aagaagtttg tccaaatgct tggttattga
     8701 actatacaaa tccgatggcg atgttaacca tgggtgtttt aaaagcgacg agtattaaga
     8761 ctgttggttt atgtcatagc gttcaagtat gcgtaccaga attgtttgag catttaggaa
     8821 tagccgatca atatgactta aatgactttc aatggaaaat tgcaggcatt aatcatatgg
     8881 cattcttgtt ggaaattaca aaaaatgggg aagatttcta tccaacaatt cgtcagttag
     8941 ctgcagaaaa accaaatcca catccagatt ctgtacgttt tgaattgttg aaacgttttg
     9001 gttactatat tacagaatca agtgaacaca atgcagaata ccatccatac tttattaaat
     9061 caaaataccc agaattgatt gacgaattag ggattccgtt ggatgaatac ttacgtcgtt
     9121 gtgaacaaca aattaatgat tgggaaagca tgcgagagga cattgtaaat aatggggagt
     9181 taacacatac gagaagccgt gaatatgctt catatatcat ggattctatt acgacaggaa
     9241 ctccgtcagt gattgcagga aatgtcttaa ataaaggctt aatcacaaac ttaccgacag
     9301 atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg cgtgcaacca acctatgtcg
     9361 gtgaattgcc aacacaatta gctgctttaa atcggacgaa tatcaatgtt caagaactga
     9421 cagtagaagc cgctttaaca ctagataaat caaaaattta tcaagcagcg tatttagatc
     9481 cacatctatc atctgaattg tctttaacag atatcaaaaa tttagtggat gatttaatcg
     9541 aagcacatgg ggactattta cctgaatata attaaaaaga agctgaaaga gtaaggaatt
     9601 ttcaattcct tactcttttc ttagttaaat ttagtaaaaa tattgacaaa acacttagta
     9661 aacagttaca ttagtgttaa caaaaagaaa acgcattcaa attgtataag gaggagcata
     9721 aaatgaagtg gtctaaaaaa attattttag cttgtatagc actaggagca acgatctctt
     9781 tgactgcttg tggaaaaaat caggaagatg caagcggtaa ggtagaaata gagtttttta
     9841 atcaaaaaaa agaaatgact caaacgattc aggagattgc taaagatttt gaagcaaaaa
     9901 accctgatat tcatgtaaaa gttgtggatg taccgaatgc tggtgaagta attaaaacac
     9961 gtatgcttgc aggcgatgta ccagatgtca ttaatcttta tcctcaaagt atagaattga
    10021 aagaatgggc gaaagctggt tatttagagg acttaaccga agaaccgtat ttagagaata
    10081 tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg tgtatatagc ataccattaa
    10141 ccgctaatgt ctatggtttt tactataaca aaactgcttt tgaggaaatg gggattcaag
    10201 caccagaaac ttgggcagaa tttgaaaaaa tagtagctga gattaaagat caaaacaaag
    10261 taccgtttgc gattgcagga gcagaaggct ggactttaaa tggttatcac caactagcta
    10321 tagcgactgc tgctggtggt gaagaggaag cgaacaatgt ttggcgtttc tcagaagtta
    10381 atggaattaa tgctgaaagc aaagaaatgc aaaaagattt taatcgatta gatttgttaa
    10441 gagaaccaaa agctttgcaa aacaactggc aaggtgccgg ttataacgat acagtagtta
    10501 cttttacaaa aggtgatgcg ttgattatgc cgaatggttc atgggcaatg ccgatgattc
    10561 attcacaaaa tccagatttt gaagtaggaa cttttccttt tccagcagat gaagcaggtc
    10621 aaagtctaac gattggcgca ggagacctag ccttatcagt ttctgcaact tctcaacata
    10681 aagaagaagc caaacgtttt gtagaatata tgacaacacc tgaagcgatg cagaaatatt
    10741 atgatgtaga tggtgcaccg tgtgctgttg agggcgttat cgagaatact caggattcac
    10801 cattaagtgg tttaactgaa cttgctttta ctgatcgtca tttagtttgg ttagccaaag
    10861 attggaatag tgaaaatgat ttttatacct taacaacaaa ttatttacac aatggcaatc
    10921 aaaaagccat gattaacgca ttaaatgctt tctttaatcc aatgaaagct gacggaaggt
    10981 aggaaaataa aatgaaaata aaagaaattt taaatcgtta ctgggcaatc atttttgtag
    11041 caattccaat tattttgcag atgatttttt tctactttcc aatggttcaa ggtgcttttt
    11101 acagtttaac taattggact ggtctgactt ataattttga ttttgtgggt ttgaacaatt
    11161 ataagattct tttttctgat ccgaagttta tcaaatcgat tggcttcact ttaattgtga
    11221 cgctttgcat gattgctgga caaattggtt ttggtatttt gattgcgcgg gcgttgaacg
    11281 caaatattaa agggaaaaca ttctttagag catggttctt ttttccagcg gtcttatcgg
    11341 gcgtggttgt ttctttagta tttaaacaaa tttttaacta tggtttacca aaaattggta
    11401 gtatgttagg cattgaattt ttacaaacca gtcttttagg aacaacatgg ggagcgattt
    11461 ttgcagtaat ttttgtttta ctttggcaag gaattgccat gccagtcatc attttcttag
    11521 cgggttttca aagtattcca caagaaatta ttgaagctgc tagaatggat ggggcgaatg
    11581 ataagcaaat tttttggaat attgaaatca cctacttatt accaagtatt tcaatggtat
    11641 ttattatggc attaaaaggt ggcttaacag catttgatca aatttttgct ttaactggtg
    11701 gtggaccaag cgatgccaca acatctttgg ggttacttgt ttataattat gcttttaaaa
    11761 acaatcagtt tggttatgcg aatgctattg ctttagtctt atttatctta attgggattg
    11821 tttcactggc acaattaaaa ttatcgaaaa aatttgaaat ctaggaggtt tcccaatgaa
    11881 aacatcaaaa gaaaaaaata tttctaaata cttgctttta ttattagggg gcttcatgat
    11941 tttagttccc ttagtttcaa cgctattcgg ttcatttaaa acaacacccg atattatgaa
    12001 taatttcttt cgttttccta atccagcgac tttggaaaat ttccgaagat tgttagcgga
    12061 tggaattggt cattactttt ggaattcggc aatcatcaca atcattgcag tagttgctgt
    12121 gacagcaatt attcctatgg ctgcttattc actagcaaga aaaatgactc gtgggaaagc
    12181 atacggattg atgtatttat ttttaatttt aggaattttt gttcctttcc aagtaattat
    12241 gattccgatt accgttatga tgagtaaaat tggtttagca aatatgtggg gattaatctt
    12301 actctatctc acttatgccg taccacagac actattttta tatgttggtt ttattaaact
    12361 aaatgtgcct atcagtctgg atgaggctgc tgaaattgat ggggctagtc actttacaac
    12421 ttatttcaaa attgttttcc caatgttaaa accaatgcat gcgacgacta ttattcttaa
    12481 tgctctatgg ttttggaatg atttcatgct accattatta attttaaatc gtgattctaa
    12541 tatgtggaca ctaccattgt ttcaatataa ttatacggga caatatttta acgactatgg
    12601 tccaagtttt gcttcctata cagtaggaat tgtcacgatt acactagttt atcttgcttt
    12661 ccaaagaagt attatagctg gaatgagcaa tggagcagtg aaataaccaa taaattattc
    12721 tcattataaa ggagttcata aacttatgat tatatttgac aatcacacac aaacatttca
    12781 tttacaaaat aaaaaaatta gctatctatt aggaatcgaa gaaaataatt atttaacgca
    12841 tctttatttt ggaaaaaaga ttacgcacta ttctggtggg tatcgctacc cccgaacaga
    12901 tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt ttattatctt tagacacatt
    12961 accattagaa tttccaggat atggacatgg tgattttaga gaaccggcgc ataatattaa
    13021 attaggaaac ggtagtcgtg taaatgattt tcgttatgat agctacgaaa ttcaaaaagg
    13081 aaagcccagc ttagcgggat tgccagcctt atatacggaa gatgatgatg aagctgaaac
    13141 gctgattatt caattgaaag acgcgattag tggattgact ttacagttgt tctacacaat
    13201 ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta gtaaataata gtgatgaaac
    13261 agtggagatt aaccgcttag ctagtcaatc aattgatttt ccaaatcgtc cactcgagtt
    13321 aattcattta aatggtacct gggcaagaga acgtcaaatg actagagaaa aaattcatac
    13381 agggataaaa gtattagata gtaaacgcgg atcaagcagc catcaacaaa atccgtttgt
    13441 tgctttagta gatgccaaaa cagatgaatt tcaaggagag gcatatggat ttaatttagt
    13501 ttatagcggt aatcacgaaa cggtgattca aaaagatcca tttgaccaaa cacgggtcat
    13561 tatgggaatt aattcattta attttggttg gcaactaaaa actggcgact catttcaaac
    13621 gccagaagtc gtgatggttt atagtaatga aggtctgaat acgttatcac agacgtatca
    13681 tgaattgtat aatcaccgtt taattcgtgg aaactatcgt ttaaaagaac gtcccacgtt
    13741 aatcaacaac tgggaagcaa cgtattttga ttttaacgaa gaaaaaatca tgaatattgt
    13801 cgatgatgcg aagaacttgg gcattgaaat gtttgtttta gatgatggtt ggtttggcgg
    13861 tcgtgaaaat gacttcactt cattaggtga ttgggttgaa acagaaggca aacttgcttg
    13921 tggtttggaa aatttagcca agcaagtgca tgataacggg atgaaatttg ggatttggtt
    13981 tgaaccggaa atgatttctg aagatagtga actatttcga gcacatccag attgggcatt
    14041 gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa tatgttcttg atttttcaag
    14101 acaagatgtt cgtgataata tctatcaaca aatgacggcg atttttgatc gtgtaccgat
    14161 tgattatgtg aagtgggata tgaatcgaaa tatgacagaa gtctattctg tcttgttgga
    14221 ctcaacgatg caaggtgaag tggctcatcg ttatatgtta gggttatatg aatttttaga
    14281 aaagttaaca acgaattatc caactatttt atttgaaagt tgttcgggtg gaggcggtcg
    14341 ttttgatgca ggtttcttgt attacatgcc acaaacttgg acgagcgaca ataccgatgc
    14401 cgtggctcgt ctgaaaattc aatatggaac cagcttagtg tatccaattt caagtatggg
    14461 tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga gaaacgagtt taactattcg
    14521 tggcaatgct gcgatggctg gtgtttttgg ttatgagcta gatctaacgc aattaacttc
    14581 tgaagaaaaa gcagaaatta aagaacaagt tgccttttat aaaaagcatc gccaactttt
    14641 gcaatatgga actttttatc gcttggaaag tccgtttgaa ggcaatgata ctgcatggat
    14701 gtttgtctca aaagaccaaa cagaagccat ggtcttttat ttccgagtat tagcagaagc
    14761 tgcgcatcca ttagtcacat tgaaattagc aggattagca gaagaagcaa cttatcaaat
    14821 ggaagatgta acatttgctg gcgatgaatt gatgaatctc ggtttttata ttgactcaga
    14881 actacatggt gattatgcga cacaacgctt ttatttgaaa cagatatagt agcttgaatt
    14941 tacaatataa gtgcaacacc aaaagagact cattccaaac gaagaaaata tgttttattt
    15001 acacgcattt actaaaaagc agcctgattt gaattgggaa aatgaagaag tacgtgaaga
    15061 aatctatgca atgattaact attggttaga taaaggacta ggcggattta gaatcgatgc
    15121 gatattaaac atcaaaaaaa aactagagta tggcatcttt gaatctgatg gagaagatgg
    15181 gttagccttc attgggccct ggattcttaa tcagtcgggc attgaagtgt ggttaaaaga
    15241 aatgagagag cggacattta aaccacataa tagtatgacg gtagcagaag ccgatgttcc
    15301 taatgaacgt ttagatgaat atattggtga agatggctat tattcgatgg tttttgattt
    15361 tagttatacg gatattgatg tacctgaaac tggtgaatgg tttaaagata gccaatggac
    15421 atggacggat atgcgtacca atatttttac gaatcaatta gttacgcaag ataaaggttg
    15481 gggcgcctta tatttagaaa accacgatca accacgttca attaataaat atattccaga
    15541 agaatggatc aatgattata gtaaaaaaat gttggcgacg ctttttatgt tactaagagg
    15601 tacccctttt atttatcaag gacaagaact aggaatgacc aacatagaaa tggcatcatt
    15661 agaagatttt gatgatgtag ctacgcattc tcagtataaa cgggcattag aatatggatt
    15721 ggcaccagag caagcattga aagctgtcaa taaacgaagt cgggataatt ctcgcacacc
    15781 tatgcaatgg aatacgcaaa aaaatgccag attctcaact tctgataatg tatggttaaa
    15841 agtaaatcct aattatccag aactcaatgc agaagctcag ttaaccaatc aacagtcggt
    15901 attcaattat tatcatcaat taattaaatt aagaaaaaat agtgtataca aagacgtgct
    15961 aatctacgga caatttctac cagtgaaaga taaaaatgaa catattttgc tttatgaacg
    16021 ccaattagaa gataaaatcg tattagttat cttaaacgtt acagcaacag aacaagaata
    16081 ttatgtagat gaacagtacc aacaagtatt aatcaataat tatgaagatg ttttattaga
    16141 tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta attatggagg
    16201 acaattatga aaattaaaaa cgaagcaatg ctcatcacct atgcagatag tttaggaaaa
    16261 aacatgggcg aattaaaaca agtcttagac aaacatttac aaggtgttat tggtggtgta
    16321 catctgttgc ctttttttcc atcgacaggt gatcgaggct ttgcgccgag tgattatacg
    16381 acagttgatc cagctcttgg aacatgggaa gatgtggaag cattaggtga agattattat
    16441 ttgatgtttg attttatgat taatcacatt tctcgtgaat caaaattttt ccaagatttc
    16501 aaaaaaaacc atgagcagtc accatacaaa gaaatgttta tccgtattca tgaatttttc
    16561 ccagaaaatc gaccaacaca agaagatatt gatttaatct acaagcgtaa agacaaagcg
    16621 ccgtttcaaa cggtagaatt tgcggatggt acgacagaag aggtttggaa tacctttggg
    16681 gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga aagaatttat tcgagaaacc
    16741 atcaaagata tggcgaacca tggctgttct ttaattcgat tagatgcgtt tgcttatgcg
    16801 attaaaaaat tagataccaa tgatttcttc gtggaaccag atatttggga acttttagac
    16861 gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat taccagaaat ccatgagcat
    16921 tattccattc aaatgaaaat tgccaatcat gattattata tctatgactt cgccttgcca
    16981 atggtcactc tgtattcact ttatagtgga aaatcagaac gtttagccaa ttggttgaaa
    17041 atgagtccga tgaagcagtt tacaacattg gatacgcatg atggaattgg tgttgtggac
    17101 gctcgtgatt tattaacaga tgaagaattg gattacacct ctgaggaatt gtataaagtc
    17161 ggggcgaatg tgaagaaggt ctattcaagt gccaattaca ataatttaga tatttatcaa
    17221 attaacagca cctattatag tgcattaggc gataatgacc gtagctatct gttagcacgt
    17281 gcgattcaag tatttgcgcc agggattcca caaatttatt acgttgggtt attagcagga
    17341 aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc gtaatattaa tcgtcattac
    17401 tatgatttag ccgaaattga agaagaagtg caacggccag tgattcaaca attatttgca
    17461 cttttaaaat ttagaaatga atctgcggcg tttgatttgg atggaacgat tgaagtagca
    17521 acgccatcag aatcagaaat agtgattaca cgtaaaaata aaaacggtag tgatagtgca
    17581 acattaacgg ctaacttgga tacgaaagaa tttatcattg agtctaaaga tataaaaatt
    17641 ttcactagtg ttgcattaaa attttaacct attccataga acaaaaaatc ctttatacta
    17701 gcacttggat gacttatcca agaccaataa aaaggactca tacatggata agtataaaac
    17761 aaatacagca tttaataaat ggttttctgc cataaaatta ggaaaacttt ctgtcaactc
    17821 ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa ctgtcttttg aagctgtgct
    17881 caagctcttt ctctacgcag ttaatgaaga aaaagaaagt ttgcgtgatt tgagtacagc
    17941 tcttatcgac cgatcgctcc aactagaaac cgatgtgtca tcgattagtc atacccaatt
    18001 gtctcgagca tttaatgcac ttgaacctaa agtgttagaa gaaatttttc aacaactgtt
    18061 agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat catctgtatc tgattgattc
    18121 cagtacgttt tcgcttagtt taaaacgtca taagtgggcg actttccgtc aaacaaaatc
    18181 aggtgtcaag ttacacttaa actattgtta tatggacgat actacgatgt atccaactga
    18241 ttttacctta acaaatgccc atgaacatga tctcaatcaa ctaccagtat tggtgaatca
    18301 accagaggca acttacgtgt ttgatcgtgg gtattaggac tttgaaaaga tggatcagat
    18361 gcattgggat ggctatttct tcgtcactcg gattaaaaaa aacaccaaag ttcacgtgat
    18421 agatactctg gaaacatcgc cagaaacaga aattctacga gacgagctgg ttcgtttagg
    18481 atctaaaacc tatctaaccg ccaatttccg actagtcact gttcaagata agaacggacg
    18541 tgtgtttcaa tttatcacca atcgaatgga cgtctcttca aaagaaatct ctgatatgta
    18601 tcacgctcgt tggcaaatcg aattgttttt caagcacatc aaacaacaca tgaccattaa
    18661 aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa ttgattttaa cgatgatttc
    18721 cgctctcctc acgtttctca tcaaattgga gacgaaaacg gaaaaatcag tgttccaaat
    18781 caaacgattc tttcgttatt tactgtttca gccctttgaa tgttgtctag aaaaattaat
    18841 tcccacatga gaaaaaagaa atttggctcg ttacttttgg ttttcagttg aataacagat
    18901 aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca gttttttttg acttattttc
    18961 agaggaaata gttttcagaa tattctaaaa tgttttatgc aacactagtg atatttttta
    19021 gaattagatt tctaatgtgc attttttgag tattaaggag caagtataca aagatttata
    19081 aagctgatta aatttgaata a
//
LOCUS       DAJSNV010000052        18064 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSNV010000052 DAJSNV010000000
VERSION     DAJSNV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.053"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            317..676
                     /locus_tag="ON516_001726"
                     /pseudo
     CDS             317..676
                     /locus_tag="ON516_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(971..2104)
                     /locus_tag="ON516_001727"
     CDS             complement(971..2104)
                     /locus_tag="ON516_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3345209.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2095..2763)
                     /locus_tag="ON516_001728"
     CDS             complement(2095..2763)
                     /locus_tag="ON516_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345210.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3219..4205)
                     /gene="lacD"
                     /locus_tag="ON516_001729"
     CDS             complement(3219..4205)
                     /gene="lacD"
                     /locus_tag="ON516_001729"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3345211.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4218..5144)
                     /gene="lacC"
                     /locus_tag="ON516_001730"
     CDS             complement(4218..5144)
                     /gene="lacC"
                     /locus_tag="ON516_001730"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCR3345212.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5157..5672)
                     /gene="lacB"
                     /locus_tag="ON516_001731"
     CDS             complement(5157..5672)
                     /gene="lacB"
                     /locus_tag="ON516_001731"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCR3345213.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5691..6119)
                     /gene="lacA"
                     /locus_tag="ON516_001732"
     CDS             complement(5691..6119)
                     /gene="lacA"
                     /locus_tag="ON516_001732"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCR3345214.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6139..6912)
                     /locus_tag="ON516_001733"
     CDS             complement(6139..6912)
                     /locus_tag="ON516_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3345215.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7077..7556
                     /locus_tag="ON516_001734"
     CDS             7077..7556
                     /locus_tag="ON516_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3345216.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7613..7915
                     /locus_tag="ON516_001735"
     CDS             7613..7915
                     /locus_tag="ON516_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR3345217.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7937..9394
                     /locus_tag="ON516_001736"
     CDS             7937..9394
                     /locus_tag="ON516_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3345218.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9987..10739)
                     /locus_tag="ON516_001737"
     CDS             complement(9987..10739)
                     /locus_tag="ON516_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3345219.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10954..11190)
                     /locus_tag="ON516_001738"
                     /pseudo
     CDS             complement(10954..11190)
                     /locus_tag="ON516_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11200..12164)
                     /locus_tag="ON516_001739"
                     /pseudo
     CDS             complement(11200..12164)
                     /locus_tag="ON516_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12370..14052)
                     /locus_tag="ON516_001740"
     CDS             complement(12370..14052)
                     /locus_tag="ON516_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3345220.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(14063..14386)
                     /locus_tag="ON516_001741"
     CDS             complement(14063..14386)
                     /locus_tag="ON516_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3345221.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(14409..15815)
                     /gene="lacG"
                     /locus_tag="ON516_001742"
     CDS             complement(14409..15815)
                     /gene="lacG"
                     /locus_tag="ON516_001742"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3345222.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            16282..17289
                     /locus_tag="ON516_001743"
     CDS             16282..17289
                     /locus_tag="ON516_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3345223.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17683..>18064)
                     /locus_tag="ON516_001744"
     CDS             complement(17683..>18064)
                     /locus_tag="ON516_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HCR3345224.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5431 a         3813 c         2981 g         5839 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgcccta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggattatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg tcgtatcaaa tgaaagctta
      421 tagaaacaaa ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt
      481 agataattca cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa
      541 agtttataac tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa
      601 taatgaacgg ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac
      661 cgttacggtt gtgtaaaaat atcattttag agcatgaaaa aaagagtgga tttctccact
      721 ccaaaaaagt ctaacttttg ggggtcacta catttttgat cctttttatt tgattaatta
      781 tatccagctg gataataatt ttgggggcaa catttctatc ttactattat cttcctaaag
      841 tagtagactt tcttattggg aaaaattaca acaaaaagga gtggcttagt tgacaactcc
      901 tttttgttta caaatcaatt ggatatctat aaattgtatt ttctaacgaa ttttatagac
      961 atctgatttg tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt
     1021 ccaatggtgt tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg
     1081 attgaataaa ggtttcatcc gtgtccctaa agtccattgt tttaggtaag ccatcctgct
     1141 tgagcccatg cactcggtac tgatgagaaa aaattgatgc ctgtttctcc tgaggaatgc
     1201 caattccctg atcttttact tcaataatca cgctaccctt ttggtttgaa atggtgacaa
     1261 ttaccagttt tccttcacca tatttgattg cattgtcaac taaattcgaa accgcccgat
     1321 aaatccaacg atctcgtcca tgaatcaggg ttggttgatc ttcagtaaac gcaaacaaaa
     1381 gattcggata attcattttg taggcatcac aaacttccgc acagatcagt gaaacgtcta
     1441 cgacttccgt gctaagatcc tctgagttat tggataatgt caaaatatcg tcaatactgt
     1501 cggtcatccg atctaaaagc attaaatttc cttgatcggc atgttgccgt tctatttcca
     1561 gattcgtacg caaaatagcg atggcatttt tctgctcgtg ggtcagataa ctatttaatc
     1621 gtcgataatc ctccaaatga ccatcgaact tatcctttag cttattaaaa gccgcattta
     1681 atgcctcgtc cttcacagta tttttaggaa cttgatcaat aaacgttaaa tcacgcgtaa
     1741 tcgctaagat ttgattactg taaattttgc gtaaaaccaa ccaaagaata aacgttaata
     1801 acatgacggc agaacaaata accagcacca tcgctggtaa atatttaaat agttcgtcct
     1861 ggtaagcttt atctatcatc gaaatagtca taaattgatt gttggaagat ttcgaagctt
     1921 ccatctgcgt ctctgcttga accgtcttac cctcttccat aaaataaaca tcttcaccta
     1981 aggtagatgc gatactgatt ccctccaaat tatttctcag aaagaagaaa ctcactaaaa
     2041 tcacgcctgt caccaacagt gaaaaagcca gcatcgttaa cgtcatttta aaatttaata
     2101 gcatagctgg tacccctttg ctcgaatggt ttttaagacc tctttttcag ctgcttcagc
     2161 cagtttcttt tttagacgag ccagatgcac tcgcagaacc gacgaaaaag gatcaaaatc
     2221 ttcatcgtag acatgttcag aaatttcctc agacgaaaca accgctggat gacgctgagc
     2281 gatgcacagt aagatatcaa attccttggg tttcaggacc acctctgtct tttgatagct
     2341 gacccttcga cctaatggat caatcttcaa agcaccaatt gaaatctgcg gcagcgcacg
     2401 tccatgataa cgacgaataa ccgcgcgtat tcgtgctttt aattctagta actcaaaggg
     2461 tttgactaaa taatcatctg ctccaaaatc caagcctttt gctcgttctt caatctcatc
     2521 acgtgcagta ataatgataa ctggactttc gacttcttca gcacggagac tttgtaaaac
     2581 agataatcca tcttttttag gcagatttaa atctagtagt atcccatcgt atgaattgat
     2641 gaaggccttc tcttctcctg actcgccatc ataagctaca tctacagaaa tattctcctg
     2701 ctctaggccc ttttttacgg ataaaaccag ctcacgattg tcctcaacca ccagtacacg
     2761 catgtatatt cactcctcat acggatttag taattacact tatgcttttt agcatagtac
     2821 acgctcttct tcaaagtcca cgttcttttt gtgaagattt gaagaaattc atacattctg
     2881 ttaaaaaaca cataattaag aatgatttta ctgtttgaaa atacctagaa tagaaaaaga
     2941 ctggaacctt tcctccagtc tcactacttt ctatttgctt ggttttcttg tatatacttt
     3001 tcaaagttcg ttggtttatg accaataagc ctcgttagaa tactgaaaat tgccatcaag
     3061 tcattaagta ccacatgtaa gaaaatctct ctaaagaaaa accccttcgt tttaactcac
     3121 ttactatttt agaattcgtt cccctaataa tgcagcatga ttttacgctt tatgtataaa
     3181 aaaagactgc aagcaatttt gcttcacagt ctgatttctt atgatagtga gcttgttaac
     3241 acttccaccg gtgtagcagt ttctttcaat acggcattta attcttcaat atttttacgc
     3301 ccttgcgtat tcatccactc catcgctgct tcttttccat caacgatata gctttctaca
     3361 ccatctgccc aagtcgcacg gccgcaaagt acaccattga aggttgagcc ggcatttttc
     3421 gcgaagcgca gtgtttcttg gaacaactct gcttttacac cggcgcttag gaaaataaat
     3481 ggcaggttag tggcttcagc ctgttccttg aagaagttag ccgcttgtgc ctttgtgtac
     3541 acgacttcgt ctgtcccata gccttccaca taattcatat tgacgggtac ttccaccttt
     3601 aacacatcta cgccataacg tggatcagaa aattccttca tcatatcgtt gactttatga
     3661 ggtttcttaa tggcgtattc tttcgaagtc gcatcagaga tgttcgcgtc ataagaaaca
     3721 agctccaaga agaagggtaa gttttcagcc tgacattctg agccgattcg ttcaataaag
     3781 gcatgttttt gattattgat ttcttctgct tcatcgacat catagtaaag taggaactta
     3841 caagcatcgg cgcctgcttc ttttaaacgt ttaactgacc agatcgataa taaatctggt
     3901 aaacggccgg gagttgacgc atcgtaacca gttttttcat aagctaacag caagccggca
     3961 ttttttgctc taacgtcagc tgctggcaag ccatattcgg gatctaataa aatcgatgag
     4021 gcatatttcg ttaattgtga agaaacaatt tctttaaatt caacgatttc tgattcttct
     4081 gcttcgctgc ccttgaattt tgtgatcatc ttcttcaaag ccccgcgttg atcaattgct
     4141 aaggccccaa tcactccatc ttcattgcat agttgcttga tatattgacg tttttcttcg
     4201 ttcatttccg tatcctccta cactttctcc actaagattt gatcatatag tttttgataa
     4261 ttggctaatt ggacatagcc agtcgtcgct tcctgagcat tcagcattcc taaaacatta
     4321 ccctgtttta acacggcttc atccgaaaga ccttgttgaa tcgcagcggt caatccggca
     4381 acggtcgcat caccagagcc aactggattg acgacatcaa tctttggaat tgtcacgcaa
     4441 taaaaagtct cgtgatgttt ggcaaaggct ccgtcgcccc ccattgaaac cactatccat
     4501 tcaatttcat caaacatcga atctgacaag gcggctttta aatctgataa ttctgttgag
     4561 atttctcgtc ctagcagacc agccagctct tcggtattgg gttttattag cttaggcttt
     4621 acactgcctc gcaacacttc ccttaatgct gtacctgaac aatctaaaac gacaggcttt
     4681 ggctgacaaa cttgcagcat ttgtcgataa aaatcagcag gcaatccttc tggcaagctt
     4741 cctgaaaaag aaatgatttc ggctttaaca gctaattttt cgaaatgttg caagaacgcc
     4801 cttgcttctt gttcagtaat aacaggtccg ttttctagga tttccgtctg ttttccgtca
     4861 tgtaaaacag cgatgcaatt acgtgtctct ccgctgatag gtaaaaagtc atgagcaatc
     4921 tcattttgag tcaggtcctt tttaatctct tcacctaaaa aaccgccgat taaaccacta
     4981 gcaatcacct ccgtatctaa ttgcttcaag atacgtgtca catttaggcc cttaccgcca
     5041 gctgttttgc gaacatttct tactcgattg actgtgtcga gcttgaaggt ttctaatgga
     5101 taagaaatat caatcgatgg attcatcgtt attgttaaaa tcatttgtcc acccgcttaa
     5161 tcgtggtatt cgccacgatc ccatttctcg ataaattcat cgaagaaatg cggatcagac
     5221 tgcttgtcac tggctttttc gacgtgatcg attttggcga tcaatttttt gttttcttca
     5281 gtttcttgat aatcggcatt gatgaaggcc tctaatatgt cacagatcaa aaattctccc
     5341 gtaatccgag cgccaaagcc gatcacattg gcatttagtt gttccttcgc atagatagca
     5401 gtcgtcatat cgcgaaccag cgcactgcgg ataccaggta ctttattgac agcgttgtta
     5461 atcccaacac ctgttccaca caaacaaact cctagatctg cttctccgct agtcacggcc
     5521 tctccgactt tttttccgta gatcggataa tgggttctgg taaagtcata ggtccccata
     5581 tccaacactt catgtccttt tgattttaag aaatctgata ctgccatttt tgtatctgtg
     5641 acgatgtggt cacagccaat tgcgattttc atttcattca tccttcctaa ttacgccatt
     5701 ttatttaaca tgtcgacacg tacttgatga cgaccaccat cataatttgc ttgtagaaat
     5761 tccttaacaa tattcaacgc caactcatcg ccaacaattt ttgatccaag tgtgatcatc
     5821 cgagaattat tatgtttacg ggtcatatac gcactgcgct catccgaaac ttctgctgct
     5881 accattcctt taacctttgt tgccgtgata aagctgccag cgccgtagcc atcaaccgcg
     5941 attcctaaac taccttcgtt ttcattcaga tcatttgcga tcgccaaagt tgattccaca
     6001 aaatcttctg aagccgtttc actcttatct actactgtaa taccttcttt gatcaatttt
     6061 ttcttgatta attcctttaa ttccatgccg tcactgtctg aaccaattac tacacgcatt
     6121 tttgtttcct cctatttttt actgaatgat tttagtgaat tgctgataat aactcttcag
     6181 ctcggctgaa agagtagaat ccgtaaataa tttgtcgatc ttgcgcaaat catagaaatt
     6241 ataaaaatcg ctatggtcga atttgtttgc atctgaaacc aaacattttt ctgccgcatt
     6301 atctaaaaca attttttgca aggtaccttc ttcaatactg aaattcgaaa cctccgaacc
     6361 cttcaatcca ttcactccga caaaagcctt cttaataccg attttttgaa cagcttcatt
     6421 ggcaattgaa ccgacaaagg ctccagtctt ttcccgatat agcccaccga tcaaatacag
     6481 ttcaaatctt tttatttcac ttaatagatt gaaaaccggc agactattgg taacgattcg
     6541 gagagatgac ggcttgatgt atcgtgtcat taattcgatc gttgtacccg atccaagaaa
     6601 aatcgtgtcg tcttcttgat tgatatgagc tgccgcattt ttagcgatgt attccttctc
     6661 ttttgtatgc agcttctttt tttcagtatg agaaagctca tttgcttcta tagaagtcga
     6721 catttccaga taatcgattt tttgtgcacc gccatgaacg cgtaaaagct ttttcttctg
     6781 cgcgagttcc tctagatctc ggcggatcgt catatctgaa acattcagta cttcctgaat
     6841 aaatttgact gttatcgtcc catagcgatc acacatcgct aagatcgaac gctgtcgttc
     6901 tgttttcagc atattctgta cctccacgac cttataataa aacatttacg aacatttttc
     6961 aacacatatt gttcgtttat tttcgtttat gcgttgacaa tgattttgta agcggttata
     7021 ctcaacttgt aaacatataa ataaacataa acgaactaat aaaggagctg ttttgtgtga
     7081 atgaattctt tttcaaagac actgtctatg tttctgacaa gcagtcgcag gaagaagtgt
     7141 tcgaggaagt ctatcaagat ctgttaaaga aagaattggt aaccgaagat tttttggaga
     7201 accttttaga acgggagcgt aattatccga cgggattaga tctctcacct gtctctaaaa
     7261 tattgccaga tatcgctatc cctcataccg aaagtgagtt tgtgcgaacc acccgtatca
     7321 ttccaatcaa agtaaaaaat gaaatccctt tctacaacat gatctcgcca agcgatcaga
     7381 tttccgcttc atttcttttc atgatcttga acgaaaatgg tgaagcacag gctggcatgt
     7441 tggctaatat catggatttc attaattctg ttgacgaaga aaaactgttt tcgtttttca
     7501 aattagagga cacagaagcg atttatcatt ttttagaaga acatttttat atatagaagt
     7561 ctctgcctaa ccgcaggata aaaaataaac ttattttaag gggagaatta ttatgatcaa
     7621 aattttagca gcatgtggag caggcgttaa ttcaagccat caaatcaaaa gcgctttgga
     7681 aactgaattg aaaaatcgtg gctatcaagt cacctgtgac gccgttatga tcaaagatat
     7741 caatgaagaa atgctgtctc attatgacat tttcgcacaa attgccaaca ccgacttggg
     7801 ttttgacatt aacatccctg tcgttgatgc aggtgctatt ctttatcgta ttcctgctat
     7861 ggctgagcct gtttaccaaa gtatggaaag cgcaatccaa tcattaaaca aataggacta
     7921 aagaaaggaa aagcatatga atacaattat tgatattgcc aataaagtat ttacccccct
     7981 aattaattta ggtgcggcac cgttaatgac catcgtatta actgttattg ctttattttt
     8041 taaagtaaaa ccgtcacgcg ctcttgaagg cgggctcaaa ttagggatcg ccctgactgg
     8101 gatcggagct attattggaa ttttaactac tgctttttca gacgctatga cagcttttgt
     8161 tgaacgcaca ggtatttcat taaacattac agatgtcggt tgggccccac tagcaaccat
     8221 cacatggggg tcgccataca cgctttattt tttactaatt atggtgatcg tcaatattgt
     8281 catgttagca atgaaaaaaa ccaacacatt agatgtggat attttcgata tttggcattt
     8341 agcaattgtt ggtttgttcg cgatcttctg cggtgctaat ctgtttattg cttctgcatt
     8401 agttattttc attggtgtat taaaaatttg gaactccgac ttaatgaaac caaccttcaa
     8461 tgacttgtta aatgcgccag acagcaaccc gatgaccacg actcatatga actacatgat
     8521 gaatccgatt attatggttt tcgataaaat tttcgacaaa atcttccctt ggttggataa
     8581 atatgatttc gatgctgcca aattgaatag taaaattggc ttctggggat caaaattcgc
     8641 gattggaatt tatttgggga tttttgttgg attactagca ggacaaacac caacgcaaat
     8701 tttctcgcta gcctttaccg ctgccgtctg tttggaattg ttctctttga taggcgcttg
     8761 gttcatcgca gccgtagaac cgttatcaca agggattacc gattttgcca ataaacgact
     8821 aaaaggacgt accatcaaca taggtttaga ctggcctttc atagcaggcc gcgctgaaat
     8881 ctgggctgcc gctaacgtat tagcaccaat catgttgcta gaagccatca tcttaccagg
     8941 aaataaatta ttgccactag gcggaatcat cgcaatgggt gttaccccag cactattggt
     9001 cgtaactcgt ggaaaattaa tccgtatgat cgtaatcggc gcgatcgaat taccattatt
     9061 cttatggtcc ggcaccttga tcgccccgtt cgttactcaa acagctaaag cagtcggagc
     9121 ctttccagcc ggcttgaacg ggtccgccat gatctctcat acaacgatgg aaggcccgat
     9181 tgaaaaattc ctaggttatt tagtcggtaa tgcttctcaa ggacaaatcg aatttgtgat
     9241 atacgctgcc ttagcattag gtgcatacct tttaatcttc atttggtacg ctcgtcaaat
     9301 gaaaaaacgc aacgctgcat acgcagctga aaaagaagag aatgcatctc cggcaacggc
     9361 gcataatatg acttatgcag ccgaagctaa ataagctaaa aacaccccga atgtctttag
     9421 acttcggggt attattttac ctataaaaaa taaatagatg taaaatccat ttcaaaaagc
     9481 atagcccatc tcttgccaca cccaataaag aaattatcaa cgtttatgct ttttttaact
     9541 cctttaagct tgcctttgtt ggtattcctc aacgattctt tttgtgttct tgtttgtttt
     9601 gtgctttttg actttttgtt gtttaattgc gaattccttg atttcttttt tcctgaatcg
     9661 cttcgcatcc cgcggttatg cgtgcgattt catcattgct ttttctgatc taactcgttt
     9721 catgtcaaag agcttcagct gacttagtgt ccatggttgt tttgatatcc cttttcctcc
     9781 ttaataaagc ggtgattgat actgctattt tttccttcac ataaacataa catcccaaaa
     9841 aaatcgacta tcttttttca aatggtgtat tcaagtattc ggaggaatta gtaatgatgc
     9901 accctaaaaa taacataatt cgcttaaatt caaggctacc aaaaaactac caagttctaa
     9961 ttatctagag tttggcagtt tctcatttac acaacatatt tttcttcttt ttcgattttt
    10021 tcatagtgct tttgttcatc atcatccgtt ataactactg ttacatcatt cagattataa
    10081 tatactgaaa aatctctttt cccaattttc gaatgatcaa tcaataaaaa gcgttcaaca
    10141 gaattattca gagcgatctg ttgagtttgg ccctcttcaa aagtcgaagt cataattttc
    10201 tgttcattta aagcattaca gctgaaaaat gctttatgaa aattcatatc ctgtagcgat
    10261 ttattcgtga tttcaccaac aaagcattga gtcgtttccc gcatctcgcc acctaataga
    10321 tagatttttc gattcccttt tcgcttattt aattcttcaa aaacaggcaa acagtttgta
    10381 acaatccgca gtgaatcccc ttgaatgatt tttgacatga gctcgattgt agttcctggt
    10441 ccaagaaata tcgtatcacc atcattgatc atttcattcg ccttttccac aatccgctgc
    10501 ttttcttctt gcagcataat ctgtttttct ctatgagaaa gctccacatg ccgataaaaa
    10561 tccttcaatt tcgctccgcc atggacgcgt tctaataatc cttgtttctc catttcgtcc
    10621 aaatcacgtc ggactgtcat atccgaaacc gaaaacttct ccataatatc gctgactcgt
    10681 actgtaccgt taacctctaa taacgacatg atcgcttgga atcgatccgt ttttaacatt
    10741 tcatcgcctc ttttcttttt aagactatca aattcttaag aaaagtgcta gaacaagttt
    10801 tcttaagaag ccttctgcag gaaggaaagc aaaatttttc agaatttgca agctgctatt
    10861 ctaaaaaata aaaccgaacg agcatgactg tcatagtgac agccttgttc attcgacttt
    10921 tatctttaat aaatcatttt atttaatctg aagttattgt gcaagttcaa aaacaaagta
    10981 gttaatgtct cctcgatatt tcgaaatcga atactcaacg ggcagacgtt ctagcgaaaa
    11041 accctgtgta tggaaataga acattggatc tccttcttcc acatctaaca aatcgctgat
    11101 cgtatcatcc gctttgatta cttctagttt tctggtgacc gttttaattg gataacccat
    11161 ttcttcaaaa acttgataca ttttttcttt tctgatttgt caaattaagc tagacaatat
    11221 gtctttatct atgcagctca aaaatacttc caatggtgtt cgataattta atgattttct
    11281 tggaatatgg tttcttttcg aggcaacgga ttgaataaag gtttcatcca tgtccctaaa
    11341 gtccattgtt ttaggtaagc catctcgtct aagcaagccg tttgagtgct cgtgctcatt
    11401 tagccctctt tgcgaaggtg ttcctggatc tgcgaaaaag atggcaatat cgttcgagtt
    11461 acttatagat cgccagttgg aaaattcttt tccacaatca aatgtcatgg atttaaacaa
    11521 gtgtttaggc atggatttga accaatcatt taacctgttt tcgatatctt cggctcgtct
    11581 gcctgtcggt ttcaaagtaa taataacctt ggataaacac tcaaccaatg taataacagc
    11641 gctcttgtgt ttaactccca caatggtgtc gccttcgaga tgaccaaatt ctttagtcag
    11701 gtgtggatag agactgttcc gttcatgaat cgttctgcga aaggcctgtt ttccacgctt
    11761 ttcttgatga ttattcggtt tacgtttccc cttcataggt aacgactgtg tgtcaaaaag
    11821 gcctttttta aacatccgat agagtgtacg aacagaacaa tcaattggaa actcgccacg
    11881 gccgataatc acatcgggtg accacccttg agctaccttt tcttgaacat atgttgattg
    11941 ttcatcagat aaaacggttg aacgtcgtcc acaacgagat ttattttgtt tgtattgacg
    12001 gtaataatct agcacttttt tccttgtttg agaaacgaat aaacattata aatggtttgt
    12061 ctagagcgat ttaaactttg tgcgacaaga gcgacaggtt tctcgatatt gaaataagac
    12121 tctatgatta ctagttcgtc tgttgtaaga tgggtgtagg tcatttgtac tcactcctta
    12181 ttaattcttt ggtcggaact attttgagtg tagcacaaat gatcttttta gttgtctagc
    12241 ttaattttac aatcgacgcc tcaaaaagaa tacccgaaat ttaaacagca aaaaaagata
    12301 gcgcttacca ttgtgtttaa gataaaacgc tagctaagtt ggctagcgct ttattaatct
    12361 aagcaagctc taaaattgct tcaatacaaa atcaactgct cctttaggat cacgtgttaa
    12421 attgatatat tctgcacctt tagttgcaat tacttttatc cctaatttgt ctgcatcttt
    12481 acggagatca tccaaatagg aattcacttg aggtgctaga acgattaaat caaaattttt
    12541 catcatatca taatgagaac cataagctcc tgctttagca gaaagattaa cacctgtctc
    12601 ttttgcgccc tcttctaatg cattagccaa cattgcactc gttccggcac cagcacaaag
    12661 tactaaaacg cgctttcctt caccaaccga tgcttttgct tcatcaacta taactgccgc
    12721 agttgcacca acttcttgga tttcttcgac caattcttct tttacttctt ctgcagcaat
    12781 gttttttaac tcttgttcta ctaattcaga atcataggcc ttaacaaaag gtaaataaat
    12841 aatcgcatcg acaacaatca atactaccgc taatacaatc gccaacacac taaacccggt
    12901 tcctaaaacc aagccaatcg gtgcgggcgt cgcccaaggc aatacggata taaagctatt
    12961 catccccaag acatcgacaa aaagtttgaa tatccatacg ttagcgattg gtgctaataa
    13021 aaatggaatt agaaagtacg gattcaaaat aataggagta gcaaatagta acggttcatt
    13081 taccgcaaac gaaactggaa taaatgaagc tttaccaact gctttcaatt gttttgattt
    13141 agcgaacaac atgaacagaa aaggaacaac tagtgtcgct ccagtaccac ccatcgttcc
    13201 aacaaagttt cctagaccaa ctgttaatac ttttgaggct tgtgcaccgt ttttaaataa
    13261 ttcaaagtta gtttctacgt tcgcataaat aattgcagca atcgctggtt caacaatcga
    13321 tggtccatga acaccaacaa accagaacat tgccatggct ccccaaatga ttgcaatacc
    13381 gacatatcca tcagcagcac taaataatgg ctgcaataat tgaattacac ctgctgcaaa
    13441 tgatgttcca aaaaattgac gaactcccgt atcaatcaaa acggcaataa atactgcaat
    13501 tgaaaagggg ataatatctc taaacatctg tgtgattgtt ccgggaactt cttttggcat
    13561 acggatcgtc aaatctcttt taacacagaa tttatacaca tttacagtaa caaacgcaga
    13621 aacgaaagct gctaataatc cctttgtccc taaataatca gtagctatcc caccatcaat
    13681 ttgatcagct gccatcatta caaaaccaca aattgacgct aacataacag aaacttcatt
    13741 aatctgctta cctgaatcag ataattgacg gttcattgaa ttagctaaac atcgagctgt
    13801 tgtagcaaca accaaaagac caactaatcc cattgaataa ccataaactt tccaaagcca
    13861 cgtcgagaat gcctctggta acgtcacgtt aaatacttct ggaacagccg caatcataat
    13921 aaaaatactt gaaaatagaa tagctggcat agcagttaaa aagccatcgc gaattgcccc
    13981 taaataaata ttacgtgaga ttttttcaaa aaacggtttt cccttctcaa tttgcttaat
    14041 aatcgtattc atttttcttc tcctatgctt tagcaaattt ttctttgtac aattcaacaa
    14101 aatcattgat aagttctctt aatagaatcg ttgtcattaa atggtcttgc ccatgaataa
    14161 aaattacgcc tacttccatt tcgttccctg acgcttcttc agctaaacat ttcgtttgtg
    14221 agttatgcgc taaggttaaa ttttcatcag catccgctaa taatcgttca acattatcaa
    14281 aattaccttc tctcacttcc ttcaacactg ttaataatga acttctggca tctcctgcat
    14341 aagcaacaat ttcaaatcct aataattgca attctttcct atccatttcc ttgccctcct
    14401 aactattttt atattatcgt ttttgtttcg ctaacaaatt tataccaata tgcagactct
    14461 ttcggatatc ttttttgtgt atcgaaatct acatagaata gtccataacg cttagtgtat
    14521 ccattaaccc aagagaaaac atccattaat gaccaaatga aatacccctt tacattaact
    14581 cccattttga tagcatcgga aatcgcatta aaatgctggc gtaaataatc gattcgatca
    14641 tcatctagaa taatcccatc ttcaaaatta tccttatacc ctagcccgtt ttcagtaatg
    14701 tatattttat tataatttgg atattcgtct tggattcgta caagtaaatc atataaccct
    14761 tttggataaa tgatccaatc ccaatcggtt ttcggaagat cctcacgcac gattctctcg
    14821 ccaatccctt taatgcaata gacttggcta cctttttctc cggtagtgtt gtagtgaagt
    14881 gtgctttctc ctttgtactc tttaacccaa tggctaccat agttattgat accaagaaaa
    14941 tcatttcgat ttgctgcttt tttcatctct ctaaaatcat tagaatcaat atctgcagat
    15001 tgcccatttg ctgctaaaat ctcattaata tgtgccattg tttcatctga gtaataccct
    15061 aaataagtag catctaataa gaacttaatt gataacgcat catctaattt cgcagcatgc
    15121 ttatcttctt ctttgtcact tgctggatat tttgtctcta gcgagtgaac aacaccaatt
    15181 tcccctttat atccaccatc tttaaaaatg tttactgtct ttgcgtgcgc atacatcatg
    15241 ttgtgtaaac aagttacagt cttctctata tcaaattgaa cttctggagg aaacgatccg
    15301 atcaaatatt gatttgaagc aactggataa atctcattaa atgtactcca ataatgaacc
    15361 tcagaaaatt catcgaaaca gaactgtgca tagtttacga aatgttcaac attttctcga
    15421 tttaaaaaat ctccattatc aaatagttct ttaggggtat caaaatgatg aagagttaca
    15481 aaaggaataa taccatgctt attcaattca gcaaatacct tatgataata ttccactcct
    15541 gtttggttca cttccccata gccttttgga aaaatacggc tccatgcaat agacatccta
    15601 atactatcaa ttttaaattt tttgcataat tcagcatcaa ctggatattg attataaaaa
    15661 tcactagctg gatcagcagt atagcgtccc ttttctacaa ggaaagtatc ccacgctact
    15721 tgcccttttc caccttcttt tgtagagcct tctgcttgat aagctgccgt agctcctccc
    15781 ataataaagt cttctggtaa agttttataa ttcattttaa tctctccttg aaaattagtt
    15841 tgatcaatat gccaataatc cataaatcac ctcagcataa ttttaacatg tataagtaaa
    15901 tttttttaca caacgaaaaa ccgacctcga atatctattt tattgcgata tacaagcact
    15961 tatatccctt gatgtaaaat aaatatacca ttcaaaaacc gttttgtaaa cgtttttttg
    16021 taaaataaat ttatcttttt actaatttaa tttacacgaa acaagaaaac ggactgattc
    16081 ccactaaaat caatcccacc tactaatcat tctccaagtg gctatccatt atcgattcaa
    16141 tcttctctgt taatggatta ggctctctgc tgatatcaga gtttcgtttc ctgctagaaa
    16201 aagtaattta agttactaaa agagcagttt cgatttactt taacaaagaa aaggattatt
    16261 atatccctag gaggatgaac aatggttact attagagata ttgcaaaaaa atctggttac
    16321 tcaacggcta ctatatcaag actttttaaa ggtgatgagt cactctctat tacatctgat
    16381 actaaaaaca aaataatcgt tacagccaaa tctttaggat atgatctgac aaaaattaag
    16441 actactctgt acaagattgc cattcttttt tggatctcag aggaacagtt aaaaaaggat
    16501 gtctactatc aacatgtcaa agatgatttg aaaaattata gtaaaaaagc taatctagat
    16561 ttgattttca tctctaaaaa cgatggtgta aaaagcattc ctgacgatat tactggattt
    16621 atcgcaatag gacaattgac aaaaaaggaa ttgtactact taaatcaaaa agggtataag
    16681 ggggtaatcc gaggaataaa cccattacct gaacacttcg atactgtaga acctgatttc
    16741 cacgaaatgg ccagacaagc aattaactac tttattaatg aaggtttcac aaaaattgga
    16801 tttattggag gaaatttctt taacctagaa acgggtaaag aagagattga tagccgtgaa
    16861 tatgccttcc gatatcattt agaaaagagc ggactactca atgaaaagta tattttctcc
    16921 caaggatcat ttactgttga cgaagggtat caattaactc ttaaaatgaa agctgagtta
    16981 aatgaagata tgcccgaggc atgtttcata ccatctgaca ctatagctgc cggcgttctt
    17041 cagggctgcc atgaattggg tattaacatc cctaaagata tggcagtaat aagtattaat
    17101 gatgacgaca tcgcaaaatt cgttgcacca ccacttacaa cttttaagat agatatcgaa
    17161 gagatagcta aaacctgtgt agatttatta agtgatcaac tggtatattc gcgggacata
    17221 tcaaagaaaa cattaatcgg cgcaaaattg accgttagga aaagttttat ccctaaagaa
    17281 gaggtgtaaa ataagtaatt gaaacttatc tttaaaacta taattgtgtc cacatagtag
    17341 ccgagcaatc gggtttatgc ctattatgtt ttccataaat aatttgatgg tgatattttg
    17401 acgtaaatac aatatgattt tttcatcgcc aaattgtgcg cgctaatttt tgtttatcat
    17461 tcagaacatt tcctcctttt tatctaagta tgaggttgga taaacgcttc atcttagtat
    17521 cttgggggat tttttatctc gttgggaata tatcaagcat cagagcgcgc aaaaagaact
    17581 ggtcgaaaaa tcaatctacc agttcaatct cattcactgt gatactatgt tttatacaga
    17641 taacgccata ttataaaaca agttgtattc cgcaaatggg ttctattcaa ctcttaaact
    17701 atctataaaa gattcagcca actccattag cctctcgtaa tttagttggt gaacagcact
    17761 tttttcaaaa aattttgaac caattcctac ataagaggct ccattgttca aatagttttg
    17821 tgcattagtc tgatctaccc ctcctacagc catcaaaggt aaacttccta aaggggcttg
    17881 cacttgattg aaatattccg gtccaagaca gttggcgggg aacaccttaa caatatcagc
    17941 acccatctca aacattttga atatctctgt gggcgacatg gccccaggaa ttgttagaag
    18001 gttctgttgc gaagtttgaa aatcaaacgc gccctctctg aactccaaat atcttaggct
    18061 gtta
//
LOCUS       DAJSNV010000053        17725 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSNV010000053 DAJSNV010000000
VERSION     DAJSNV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.055"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            199..1044
                     /locus_tag="ON516_001745"
     CDS             199..1044
                     /locus_tag="ON516_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3345225.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1235..2422
                     /locus_tag="ON516_001746"
     CDS             1235..2422
                     /locus_tag="ON516_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3345226.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2428..2784
                     /locus_tag="ON516_001747"
     CDS             2428..2784
                     /locus_tag="ON516_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3345227.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2786..3124
                     /locus_tag="ON516_001748"
     CDS             2786..3124
                     /locus_tag="ON516_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3345228.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3200..3456
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3519..4553
                     /locus_tag="ON516_001749"
     CDS             3519..4553
                     /locus_tag="ON516_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3345229.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4546..5232
                     /locus_tag="ON516_001750"
     CDS             4546..5232
                     /locus_tag="ON516_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3345230.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5256..6104
                     /locus_tag="ON516_001751"
     CDS             5256..6104
                     /locus_tag="ON516_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3345231.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6301..7074
                     /gene="sufC"
                     /locus_tag="ON516_001752"
     CDS             6301..7074
                     /gene="sufC"
                     /locus_tag="ON516_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3345232.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7087..8373
                     /gene="sufD"
                     /locus_tag="ON516_001753"
     CDS             7087..8373
                     /gene="sufD"
                     /locus_tag="ON516_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3345233.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8370..9605
                     /locus_tag="ON516_001754"
     CDS             8370..9605
                     /locus_tag="ON516_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3345234.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9592..10062
                     /locus_tag="ON516_001755"
     CDS             9592..10062
                     /locus_tag="ON516_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3345235.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10067..11458
                     /gene="sufB"
                     /locus_tag="ON516_001756"
     CDS             10067..11458
                     /gene="sufB"
                     /locus_tag="ON516_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3345236.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11553..12695)
                     /locus_tag="ON516_001757"
     CDS             complement(11553..12695)
                     /locus_tag="ON516_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345237.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12734..13468)
                     /locus_tag="ON516_001758"
     CDS             complement(12734..13468)
                     /locus_tag="ON516_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345238.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            13700..13975
                     /locus_tag="ON516_001759"
     CDS             13700..13975
                     /locus_tag="ON516_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345239.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            14073..16070
                     /locus_tag="ON516_001760"
     CDS             14073..16070
                     /locus_tag="ON516_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345240.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16146..16481)
                     /locus_tag="ON516_001761"
     CDS             complement(16146..16481)
                     /locus_tag="ON516_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345241.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            16608..16823
                     /locus_tag="ON516_001762"
     CDS             16608..16823
                     /locus_tag="ON516_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345242.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            16860..17222
                     /locus_tag="ON516_001763"
     CDS             16860..17222
                     /locus_tag="ON516_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3345243.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            17268..17594
                     /locus_tag="ON516_001764"
     CDS             17268..17594
                     /locus_tag="ON516_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345244.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         5828 a         3071 c         3720 g         5106 t
ORIGIN      
        1 taccaagatg aacgaataca ttgtaaaatg aataggaatg tatgattacg agcaagcaga
       61 cagctgattg tcttcatcta agttactagt tggatcagca actgttttgt tgtacatttc
      121 tattatagat ggttttttgt atatttaaat caaatagttt ttatagtaat tatcataaaa
      181 agttatggaa gtggtgccat gtttaagtta ttaacgactt ttcgagcagt atatgaaaca
      241 agaaattttt ccagagcagc agaagtactg tttttatctc aaccagcagt ctcgaatcaa
      301 atcaaacagt tggagcggga attgaatatc gatctttttc aacgtaacgg aagaaaagag
      361 atgcttccaa ctaagcaagc cgacttatta tatgaacgga cactggtttt gttagaagaa
      421 tgggaagaaa ctcagcaaaa attgctcaat gaacaaaatg aagaagaagt atgtcggatc
      481 gttgcttccc acacctttgc tgtctatctt ttgcctcagt tgatgaaaca tctgatcaaa
      541 aggtttccta aagtgcattt tgagatcgaa atcacgaatt ctcatgaatc attagaacaa
      601 gtagcgaaac atgaatccga ttttggtttt atcgaaaagc cattagagac ttccggcgta
      661 caaagatact ctttgatgga agaccagcta gtcatcgctg gggacccaaa aagcaaattg
      721 tggcttgtaa gagaagcaaa ctctggtgtt tttcattaca cgcagcgtta ttttgaagag
      781 atgaatatcc aagataagaa gatgatcgtg aaaagtaacg atgtaattgt tgcgttgctg
      841 aaggaaggaa tcggtcgctc gattttgtct aagcgctctg ttccagaaaa tgtgccaatg
      901 actccgttga atttccatcg atatttttat ttcatccaac gaaccaatct ggtttctgat
      961 cagctgctag agattgccga agagatcctt cgatacaatg aaagcacaga aacttctcac
     1021 agcgaggaga atctcagctg gtagataatt gcatgtatca aaaaaagttc tatgatacac
     1081 taaagataag cagtaggctt ttgtactgca tttgctcaag caactgcaca aacggaaagt
     1141 taatttttcc caaatgtcta tggaaagact ttccattatc taactggacc gctataataa
     1201 tcagcagtat ggtttttaag aaaagggatg aaacatggaa aaaaaattaa aagcttcgaa
     1261 cgataatcgt attgattatg gcgtgatttt accagtcttt ctgttatgtt tgattggtct
     1321 gctctcatta tatgtagcac ttagccatga tccgaacaat ccagcagtct tgaaaggcgt
     1381 agggatgcaa gttgtctggt atttagttgg agcggcagca atcgttgtca tcatgcattt
     1441 gaattccaaa tggatctgga aactgactcc ttacctttat ggtttggggt tagtggtaat
     1501 gggactcttg ctcaaattct atgatcagaa tttagctgct tcaacagggt ctaaaaactg
     1561 gtttcgattc ggtgcgttta ctttccagcc agcagagttg atgaagattg cgtacatctt
     1621 gatgatggca ctgatcgtaa ctaaacataa tacgcaagta aaagaacgga cgatgaaatc
     1681 ggacttttgg ctgattggga agctcttgat cgtgacaata ccggttttag cattgatcat
     1741 ggcccaagat gactttggaa cgatgcttgt ctttcttgcg atctttggcg ggattttcct
     1801 gatgtctgga atctcttggc gcatcatcgc acctgtagta atcttggcag tagtcgttgg
     1861 agcaggaacg atcttcttgg ttacgactga aggcggaaga gacttgcttt ataaagttgg
     1921 attcaaatct tatcagtttg cacgtattga ttcatggctg gaccctttcc atgatacgtc
     1981 aggaatgagt tatcagccgg cccaaggact tttggcaatt ggtaccggtg gcttgtttgg
     2041 taaaggattc aacgtaagca atatctatgt gcctgttcgt gagtccgata tgatttttac
     2101 cgttatcggt gaaaacttcg gctttatcgg tggtgcattc gtcattttct tgtattttat
     2161 tttaatctat cgtatgattc gggtgtgttt cgatacgaat aatgaatttt acgcctatat
     2221 cgcaagcggt ttgatcatga tgctgctgtt ccatgtcttt gagaatattg gagcaaatat
     2281 cggattactt cctttaacag ggatcccatt gcctttcatt agtcagggag gatcctcgat
     2341 tttagggaac atgatcggta tcgggttgat attatcgatg agataccaaa atgaagcacc
     2401 tgcaagatct tcaaggaggt aagaaaattg tacacctttt actggtaccc gaaatgttca
     2461 acatgcaaaa aagccaaagc gtggttagac cagcatggcg ttagttatca aaccgttgat
     2521 atgatcaaag aagtaccgaa ggcctcaacg attgagactt ggctagaaac gagtcaagta
     2581 ccaatccgtc gcctgttcaa cacaagcggg atgaaatatc gggaacttgg actgaaagat
     2641 caagtaggcg agtttacggc ttcaaaagcg agccaagtct tatcgacaga tggtatgctg
     2701 atcaagcgac cactgatctt acgagaggac cagcttgttg caattggatt caatgaaaaa
     2761 atttatgaag gagaactgat ataaaatggc aggcaagcat ttgaagaaaa aggacaacct
     2821 ttggatctta tttaatggaa aagaatactg tgttggtttg acaaatgaag cgcaagaaga
     2881 acttggagac atcacgtttg ccaatctgcc aaaagcagga caatcgctaa aaaaagatga
     2941 acctctgatc gaagttgaag cggaaaaagc ggtcaatgaa tttgccagcc cgctaacggg
     3001 tacagtttct tctgttaatg ataaaatcgg tgaagatatc aatatcttga atgattcaga
     3061 tgaaatgaat gcgtggatct taagtttcaa agatgttgat ccaaaagaat ttgacgaatt
     3121 ataaaatcag aaaatcactt gaaaaaagtt gacactgatt cttccagttg ctataatcat
     3181 aagcaatcct atatgaaacg agaaagcttt gaaaagaaga gtacatgtca cggacgtttg
     3241 aagagagccc tgtttgctga aaatgggtaa cgaaaatgag atggaagatg gtcttagagc
     3301 agagtaagcg agcgtaagca aacttactac gggagtgccc gttacagcgc gacagtatac
     3361 ggaaagaagc taagtccaag tactggaaga ggctgttatt gcgaagtaac ggtaaatcaa
     3421 ggtggtaaca cggaagttca agctttcgtc cttttgctta aggcaagagg gcgaaggctt
     3481 ttttgtttga aaaatcataa gagaaagatg tgaatggcat gtcattaatc caactaacga
     3541 atgtcaaaaa gacattcaat gggaaaaacg gaaacatcca agccgtcaat gacgtttcac
     3601 taaacgttga aaaaggggat atttatggaa tcgtcggcta ttctggcgca ggaaaaagta
     3661 cattagtccg tctcctgaat ggacttgagc ttcccacgag cggcgaagtg atcgtaaacg
     3721 atcaagatat tacaaaatta aaaaacaaag aattacgtac attccgaaag aaaatcggta
     3781 tgatcttcca gcatttcaac ctgttatggt caagaactgt acttgaaaat atccagctgc
     3841 cgctggaatt agctggcgta ccaaagagca aacgtaaaga acgggcagaa gaactgttgc
     3901 gtttagtcgg gttagaagga cgcggacaag catatccttc acaactttcc ggcggacaga
     3961 aacaacgcgt cggtatcgca cgagcattgg caaatgatcc tgagatcctg ctttgtgatg
     4021 aggctactag cgcattggac ccgcaaacaa cagaagaagt attagactta ttgttagcga
     4081 tcaataagaa attgaatttg accatcgtct tgatcacaca tgaaatgaat gtgatccgca
     4141 aaatctgcaa caaagtcgct gtgatggagt tggggaaagt cgtagaagaa ggagacgtat
     4201 tgactgtctt cagacaaccg aaaaaagaag taaccaaacg ctttgtacaa caagacatcg
     4261 aaccagaagc tgattcgaca gaactattag ctgaactcat caaagaaaat ccaagcggcc
     4321 gtttggcaac tttgaccttt agcgaaagaa atgccaacga gccagtcatt tcccaagcga
     4381 ttcgacgcta taacatcgat gtcaacgttg tgtatggaaa gatcaaacaa gtgaaagaag
     4441 gttcctttgg ttcattgact gtcttgatga ctggtgaaac caaagaatta gatcaagcag
     4501 aagtgttcat caaagaacaa ggagtaggaa ttgaggtgat ccatcgtgga taaaagcttt
     4561 tcagaaacat atctagattt taaacaaatc aacccggaaa cgatgaatca ggctatcctt
     4621 gatacactat atatgacatt aatctcaatg gtcttagtga cgatcatcgg attagttttg
     4681 ggattgatcc tttactctat tggacgcaaa aacaaaccgt atgctcgaat catttatgga
     4741 atcgtatcga tcatcagtaa tattttccgg tcaacgccat ttatgatcct gatggttttg
     4801 atcattccat ttactaaagc aatcgtaggc actatgctag gtgcgcaagc agccattcca
     4861 gctcttgttt tatccgcagc accattttat gcccgtctag tagagattgc ttttcgtgaa
     4921 gtcagccctg gtgtactgga agcagcagat gcaatgggag caagttactg ggaaatcatc
     4981 tggaaggtct tgatacctga aagtattccc gcattgattt ctggattaac agttacaacg
     5041 atctcgatga tcggatttac tgcgatggcc ggcgcgatcg gcgcaggtgg acttggaggt
     5101 cttgcttggc aggaaggcta ccagcgtggt aatctaacag tgacattcgt tgcgacttta
     5161 attattttac taatcgtatt tatcgttcaa ggaatcggtg atttcttaac taagaaaaca
     5221 gacaaaagat aataggatat agggggatgt aggatatgaa aaagaaaatt tttggatttg
     5281 cagcagcgct tttgctgact gtaggattag cagcatgcgg taacaacagc gacagcaaag
     5341 attcagcaaa taaagcagac gacacgacat tgaaagtcgg cgcttcaccg acaccgcacg
     5401 cagagatttt ggaacatgtg aaaccattat tgaaagatga aggaatcgat cttgaaatcg
     5461 tcaaattcga tgactatgta ttaccgaacc aatctttgga agaaggcgac attgatgcca
     5521 attacttcca gcatattcct tatttgaaca aagaaatcaa agaaaaagga tatgactttg
     5581 tcaatgccgg tgctgtccac atcgaaccaa tgggcttgta ctctaaaaaa gtcaaagatg
     5641 tttccgaatt aaaagatggt gcaacaatca ttacttcaaa ttctgaatca gactggggac
     5701 gcatcatcac aattctacaa gatgctggct tagtcaaagt caaagatgga gtagatctgg
     5761 aaacagcaac attcgatgat attgaagaaa accctaagaa cttgaagttc aaccatacga
     5821 tcgacccatc acttttagct tctacgtacc aaaatgatga aggtgaccta gttgcaatca
     5881 acgcaaactt cgcttacggc gcagggttga atccattaga agatgccgta ttgcttgaaa
     5941 aggacaactc tccttacgtg aatattattg cgactcgtaa aggtgatgaa gacagtgaca
     6001 aaatcaaaaa actgattgaa gtgcttcacc gcaaagaagt ccaagaatgg atcacagaca
     6061 aatggggcgg ctctgttaaa ccagtaaagg ctgatgcaaa ataaggtatg gaacaaaaaa
     6121 gatgagaaat aagtctgtaa tggcttattt ctcatctttt ttatacctat tctaatttag
     6181 aaatcaaaat tgtttaataa taaataaaag atatttgatt ttagtaagga aggtcattgc
     6241 gcttcattct caattagtct aaaatgagtg agaacgaaag agaatttggg ggtataatgc
     6301 atgtctgtct tagaaataaa aaatctacat gtgtcgatcg aggaaaaaga aatccttaaa
     6361 ggcgtaaacc taacaatgaa aaccggagaa atccatgcca tcatgggacc taacggcaca
     6421 ggaaaatcta ccttatctgc cgctatcatg ggcaatccaa actatgaagt aacagaaggc
     6481 gaaattttgt ttgacgggaa aaatgtttta gacttggaag tagatgaacg tgcgcgactt
     6541 ggcttatttt tagctatgca atatccaagt gagataccag gtatcacgaa tgcggaattc
     6601 atgcgtgcag cgatcaatgc gaaacgtgat gaaaacgaca aaatttctgt tatggcattt
     6661 ttgaaaaagt tagatgaaaa aatggcttta ctaaatatgc cagaagaaat ggctgaacgt
     6721 tatctgaacg aaggtttttc tggcggagaa aagaaacgga atgagatcct gcaattgttg
     6781 atgttagaac ctacatttgc aattttagac gaaatcgatt caggtctgga tatcgatgct
     6841 ttgaaagtcg ttgctaaagg agtaaatgaa atgcgcggag acaatttcgg tgcattgatc
     6901 atcacccact atcaacgatt attgaactat atcacacctg atgtcgttca catcatgatg
     6961 gaaggccgag tagtcatgac agggaatgct gacctagcta aacgtctgga agcagaaggc
     7021 tacgctggta tcagtaaaga attaggaatc gactataaag aagaagaagc gtaaggggga
     7081 gcaagaatga aagaaaaaaa ctggctcgat tatcttgacg cggtaaatga tttttcttta
     7141 agtaaaggcg aacctgattg gatgagaacc ttccgtcaag acgcactagc gaaagcagat
     7201 gaactgccat tgccgcatat cgatcgggtg aaattccatc gctggtcgtt atttgatgtg
     7261 aaagaaacac aaaccatcag tgaaacaggc acgattccgg catttgatgc aatgaaagac
     7321 aatcctgtgc ttgtccaaca aggttcatgg acgatttttg aacaactgcc tgtggaatta
     7381 gcagaaaaag gagtcatttt taccgacttg ttcactgcaa tgatcgaata tccagaacta
     7441 gtccaagaat attatatgaa aaaagcagta aacatgaacg aagaccaatt gactgcactc
     7501 catgtagctt ttatgaacag cggtattttc ctgtatgtgc ctaaaaatgt cgtgatcgat
     7561 gaaccactcg agtccttatt cattcaagat ggcgcatccg atgagcactt cttcaagcat
     7621 gtgttgatcg tggcagatga acatagtgaa ttttcttatc tggaacgttt ccagacaacc
     7681 aaagaacaag tagcaaaatc atcaggtaat atcatcgtgg aagtcatcgc taaagctggt
     7741 tcaaaaatca aatattcagc cgtagatcaa ttaggagaaa atattacttc ttatatgaat
     7801 cgccgtgggc acattttacg agatgcttct gtcgactggg caattggtgt catgaatgac
     7861 ggtcatgtga tcgctgactt tgactcagat ttagcaggag aaggtgcaca cgcagaagtc
     7921 aaaatcgtgg caatcagctc gggcagacaa atccaaggaa tcgatacacg agtaaccaat
     7981 aaagcccctc atacgatcgg gcatattttg caacacggag tgatccgaga aaaaggaaca
     8041 ttgaccttta atggtatcgg ccacatcttg aaaggggcaa aaggtgcaga tgcccaacaa
     8101 gaaagtcgtg tcttgatgct ttctgataaa gcacgtggtg atgcgaatcc tattcttttg
     8161 atcgatgaaa acgaagtaac tgctggacat gctgccagtg taggacgagt tgatccagaa
     8221 gaaatgtact atctgatgag tagaggattg cataaagaag aagcagagcg cttagtgatt
     8281 cgaggattcc tcggttctgt cttaacagca atccctgtag aacaagttcg gaaagaatta
     8341 gtcgaagtaa tcgaagggaa gttgaatgga tgatcgatgc taaaaagcta cgcaatgatt
     8401 ttcctatctt atttcaagac gtcaatgatg aacctttagt ttatctggat aatgcagcga
     8461 cgacccaaaa gccaaaacaa gtactccagg cattagcgta ctattacgaa cacgataacg
     8521 ccaatgttca tcgcggtgtc catacgttag cagaacgcgc gacccatgat tatgaacaag
     8581 caagagaaaa agtacgggcg ttcatccatg caaaagaaac agcagaagtg ctgtttactc
     8641 gtggtacgac gacaagcttg aattgggtag ccagaacctt cggcgaccgc tatatcaaag
     8701 caggagacga gatagtgctc tcttacatgg aacatcattc taatgttatc ccgtggcagc
     8761 agttggcaaa acgaacaggt gctgtcttga aatatatcga actaacagaa gaaggcttcc
     8821 tggatatgga ggatgcagcg aaaaagatta cggacaaaac caaaatcgtc tccattgctc
     8881 atgtatccaa tgtcttaggt gtcttcaatc ctgttcaaga attagctgaa cttgtccatg
     8941 aaaacggcgg ggtcatcgta gtagatggtg ctcaatctgc cccgcacatg gccattgatg
     9001 tccaagcact agactgtgat ttctttgctt tcagcggaca caaaatgtgc ggaccaacag
     9061 gtattggtgt tttatatggt aaacgaaaat ggttagaaga aatggaacct gttgaatttg
     9121 gtggcgaaat gatcgatttt gtcgaattat acgacagtac atggaaagaa ctaccttgga
     9181 aattcgaagc agggacacca aatatcgccg gcgctatcgc attaggccat gcaatcgatt
     9241 atttgcaaaa actcggtatg gacaatatcc atcgatatga agaagaacta gccgcttatg
     9301 tgttgccaaa attacaagca attgatggat tgactgttta tggtccacaa gacccgaatt
     9361 ttcggacagg agtcgttgca ttcaatttgg atgggttaca tccccatgat gtcgcgacag
     9421 ctttagacat ggaaggggta gccgtcagag caggtcatca ttgtgcgcaa ccgctattga
     9481 agtaccttga agtatcagcg actgctcgcg cgagctttta tttctacaac acaacgcaag
     9541 acgccgaccg tttagttgaa gcaatcatgg caacaaagga gtttttccaa catgggacta
     9601 tctaaattag acaatttata ccgtcaagtg atcttggatc attcttccca tccgcatcat
     9661 cgtggaagct tgactgactc gacaagcaag atcgaattga acaaccccac atgtggagat
     9721 gtgatcgaat tgcaagtcga tgtaaaagac ggaatcatca aaaatatcgc ctttgacggc
     9781 agtggctgct ccatcagtac ggcaagtgcc tcgatgatga cagacgccgt catcggaaaa
     9841 tcaatagaag aagcagaaca tttaactgta gactttctac agcttgtcca aggaaaagaa
     9901 gtcaaagatc ccgaccaatt aggcgatgct gctatgcttg gtggtgtagc taaatttccc
     9961 gctcggatca aatgtgcgac acttgcctgg aaagcactag gaaaagcaat ggaaaacgat
    10021 ggtaccgcag ctttccatca ttaccacaag gaagaggagt gagaacatgg gcgtaccaga
    10081 attagaagaa tataaattcg ggtttcacga tgatgtcgaa ccggttttca gtacaggaga
    10141 aggcttgaca gaagaagtga tccgtgagat gtcaagaatc aaaggcgaac cagaatggat
    10201 gcttgaattc cgtctgaaat ctctagaaac atttaacaaa atgccaatgc aaacatgggg
    10261 ccccgacctt tcagacatcg atttcgatgc catcaaatat taccaaaaac caagtgacaa
    10321 acccgctcgt gactgggaag acgtaccaga aaaaatcaaa gatacatttg aaaagattgg
    10381 tataccagaa gctgaacgag cttatctagc cggtgcttcc gcacaatatg aatcagaagt
    10441 cgtgtaccat aatatgaaag atgaattcga aaagttagga atcgtcttta ctgacactga
    10501 ctctgctttg aaagaatacc ctgaattatt caaagagtac ttttcaaaac ttgtaccgcc
    10561 aaccgacaac aaattagcag cgctgaattc agccgtatgg tcaggtggga cctttattta
    10621 cgtgccaaaa ggcgtcaaag tcgatgttcc attacaaacg tacttccgga tcaatgcgga
    10681 aaacacgggg caattcgaac gtacgctgat cattgttgac gaaggagcaa gcatccacta
    10741 tgtcgaaggt tgtacggcac ctacttattc aagtaacagc ctgcatgccg ccatcgtaga
    10801 aatctttaca agaaaagatg cgtattgccg ttacacaacg atccaaaact ggtcagacaa
    10861 tgtgtacaac ttagtaacca aacgagcaaa agcatacgaa ggcgcaaccg ttgaatggat
    10921 cgacggaaac ctaggtgcaa aaacaacgat gaaataccca agtgtttact tagatggaaa
    10981 aggtgcacga ggcaccatgc tctccatcgc ctttgcagga gcaaaccaaa tccaagatac
    11041 gggtgcaaaa atgatccaca atgcaccaaa tacatctagc tctatcgtct ctaaatcaat
    11101 ttcaaaagac ggcggagaag taaactatcg aggacaagta acctttgcca aaaacagtgc
    11161 gggttcgatc tctcatatcg aatgtgacac gatcatcatg gacgaatggt cgaaatccga
    11221 tacgatccca ttcaatgaga tccacaacag ccaagtctct ttagaacacg aagccaaagt
    11281 ctcaaaaatc tcagaagaac aactctacta tctgatgagc cgaggcctga ccgaatctga
    11341 agcaactgaa atgatcgtca tgggctttgt tgaaccattc accaaagaac ttccaatgga
    11401 atatgcagtc gaactcaatc gattgatcag ttatgagatg gaaggcagtg ttgggtaaac
    11461 ctgttatagg agctagaacc cttgatatat aagggttcta gcttttttgt tttgcctgta
    11521 tactagaaat gaccagattt tgaccagctc ttttactgat gagccatcat atcagcaaag
    11581 tcttgacctg ttttcttagg tgcattattt gtaatatgtg catagatgtc taatgtcact
    11641 ttactatttt catgccctaa tcgttgttga actaccttga cagattctcc tgcttctaat
    11701 aataaactag catgtgtatg tctaaaccca tgaggtgtaa tacgaggcaa gttatatttt
    11761 ttcaaaatat atcttaacca atcattaaca gtgtttggaa tgtatagagt gtttttaact
    11821 gttgtaaaaa catgttgctc tttagagcta gtattgtatc caagttttaa atactcttct
    11881 ttttgctgta atttccagtt gttgaggata gaaagagtct cagtatctaa agagataact
    11941 cgtctagaag ctcttgtttt cggagattga attataactt taccaaattc atctacagca
    12001 agagttttat tgacatgtag ttctttatta aacgtgtcta tgtctttcca ctgtaaagcg
    12061 agtacttcac tctttctcat acctgtgtat gccaataatc tgaagaaggc gtattgtttc
    12121 atgttaccaa aatctttaaa agcatccagt aaattattaa gctcctcttt tgagtaaaat
    12181 ttacgatttt tctcatactc tttttttcgt ggaagaagtg ttttttccat aggattattg
    12241 tcaatatatt gcattgcaac cccgtagcgt aaaatttgtg cagtctgttt tctcatataa
    12301 tcgtaacttt catatttact gtgccattca tctaccactt tttgacaata tgggacagag
    12361 atattcgata gtttaagctt accaaatgct ggaagtattt gattatttgc gtatcttact
    12421 gagattgcga ttgttgaagc tttaacactg gtacgctgat gagttatcca ttcattatac
    12481 aggtcttcaa atgtcatatc aggtgctttc tttttaacac cgtataatat ttgatgtctg
    12541 tactcgtcat aatctttttg agcttcttgt ttgctactaa aacctcttct cgtagtattt
    12601 attttttcac cagttcgtgg atcggtggca atgtgtcctt tatacatcca cgccacttga
    12661 ccgtttttct ttttgtactt tgatattttt gccattaaat catctcctct tagtttacaa
    12721 ggagtactta ccactagatg gtatcacctc ccttcatttt tttcttagtg tattttttcc
    12781 caattgatga aatcgtttgt tctagcttgt ttatattatc agtaatttct ctcacagctt
    12841 cttcgggata agacatgtct ttcaattgaa taggtagcca ttcatctacc attctttcta
    12901 gtagttcttc tatactgcta gtgaggaaaa gaatgttatt gtcaaattct tctacaatag
    12961 cattgatgaa taattcttct atttttaaga tattgaaaag actaattttt tcatttttaa
    13021 ctttattaca agtcttgtta gcaataaatt cttgcatctc tggttctaat ttggaatatc
    13081 tatccagcca tgtatctaaa ttagagtgta ctggtgggaa ctgttgacga agatgctgga
    13141 acaggttttt atctggaaat gttataccat ctagtacaat gctatctcta taaagaataa
    13201 ttcccaaaat atattcttct acattgccac aaactaactc ctctatactc atatttccca
    13261 aatcagcaat tcgggataat cgctctggta ttggtaaata ggttcccttc tcccattcgt
    13321 agatagtttt tttgtcaatt aattgaccgt tgttaatcat aagaccaaat gctggttgtg
    13381 ttaatttttt actctttctt atagctttta tttttgttcc gagtatcttt tttcgttcta
    13441 aataatttat tggtggacgt tttatcatat tgaccacacc tcctaaaatt agtatacctc
    13501 aaaaaagggg tgttgtaaat aaattttaca aagctgttga cacccctttt ttatcgaggt
    13561 attatgtgta tgtcgatgaa ctggtaatca ttttttgttt gattaatcgg ttaataacaa
    13621 aatactttta ttttgagcaa aaaatacgaa gataattctt agaaaatttg ttcaagcaat
    13681 cacgaaagga gggggaaata tgtctctaca aatcattgat agcattgttt ctatacaagt
    13741 tgaacaacaa gtgaaagaga taattacagt gctaaagcct atgctagaag acattatttc
    13801 tggagtcact ctagagtata tggactatca gcaggcaagt gaatatctag gtgtatcagt
    13861 aggaacgatt agaaaatatg tttctcaata cgggttacct gttataaaaa ttgatacggt
    13921 cattagattt aaaaaatctg atattgatag tttcatggaa cggtataaag gttgagtaaa
    13981 caaataagac taaaacatat agtaataaag agtttgtttt tattatctgt atgttttagt
    14041 ctttaacaga attttataag gaggaaacac aaatggaaac atcaatgatt aatattccaa
    14101 tttatgtaga tcaagaaaaa ttattagaga agccagttac aaaaaaagaa aaaactgatg
    14161 taagagatag gtgtttagat aactatagag aagttacaag agatgagttt gtagagatac
    14221 taaattcatc acaatcattt attccaagca agacaaagaa taaaggcaat gacaaggaga
    14281 gttttgtgga aaccagagta attattctgg atgtcgataa cactgttaaa gatgagaagg
    14341 ggaaggtagt agatttgagt aaagatgata gtagatactt aagcatagaa aaggttttat
    14401 ctatcaattt agttcataat tctgcgtttg cgatacaaaa aagtattcgg tactctgaaa
    14461 atctagaaaa atacaagatt gtctttctat taaaagaagc aattactgac tatggtaaaa
    14521 tggttgctgt ttacgaatac ttgaaaaaga acatccccgg gtgtgatgac aatgtaaatg
    14581 cttcgaatag aatgtttttt ggaggatata agtcagatgc agtgataata attaatgaag
    14641 acaacatgtt agatattact gagcttccga ttgattttac catgacatgt tctaaaaatt
    14701 gtcaaagtga ttcagggaga gagtatgaac tagtcaatga aaccgatttt gttaagttga
    14761 taaaaaatga cgacaaagaa gaaatgaaac agtggtttaa agactgcctg tttgattcct
    14821 ccgacatgtc ttttaatgag atttatgaaa gattgttaac cattgatatg aataaattac
    14881 ttaagtcaag taaaaactta agatgtctgt ttcacaacga tcataatccc tctgctagta
    14941 tttttaccag taaaactggg cattccattt tttattgtca ttccagtaat tgtgatatca
    15001 gtgaaaattt tattggagtt gtgatgctaa ttttaaagaa agacagtcgg gtagaaacat
    15061 ttaattggct attagagaac ctagacttgt atcctacaca ttttcgaaaa ttgaaagagg
    15121 aatcaaaagt actttttgac actcttgaat caatgaaaga caaacttttc aggacaataa
    15181 ggaattatct agaggaaatg cgacgtgtct atgatgtctt gctttctgat gttaattttt
    15241 ttgatgatag taaggaaagt gtgacttgta ttttgtcggg ggaacaatta gctaaaaaaa
    15301 tgagttctta ctatggtaaa acttatgaca tagataaatg taataagtta ctatcactta
    15361 tgacttttat aggattgata aaaaaattag atgatgaaga tgttcctttg aagatgctcg
    15421 aatacctaaa taggttaaag gaagagaaag cgttaggaaa ctttaatcat aagagaagca
    15481 atgtctatcg attagacgtt tttaatgcaa cttctgtgat tcaaaaattg aatgaagata
    15541 tccttccact tctggaaaaa attcatttta catatcaata tttttcttat gaatgggtta
    15601 agatatgttt taacgaaaaa gaggctaaaa gagtatttcc tcaaaattcc cgatcagaac
    15661 tcagcaagga aaaacaattg attttagatg aagcacatga agttttacag ggcttatcgt
    15721 ggcaagggac atatctaatg acagagaaag aacttattaa cactgtttgt gaacgcctac
    15781 gtaatgttgg ggataacagc gtgaaaaatg ctttaataaa aaacagaggc tactttgtta
    15841 aacaaggatt cactcttgaa cgagcttcta aggatattaa gaaactatac aatgtgaaag
    15901 caagtagtag ttttttaata tatttagcga aaggggcagg gatagatgaa acaataaaag
    15961 aattgatatt atcaggagct gggatagttc ctctgtatca atttacaagt gtaacaagag
    16021 atgatggacg gagagtaagg aagagaaaag acccaattgt attaaactag acaggttatc
    16081 catagctctt aatcttattt catctcaata cacccaccgt taaagttcgg tgggttattt
    16141 atttttcaat ctttcaatag ctgattgcgt tcattttctt gcctttttaa tagcttcata
    16201 tagtcaattg ttcgaactgc atcttcagtg ctaagagaac gatagagagc caaaaactct
    16261 tcctcataat ctacgacaac attgtctaga aaatggctta caggggttcc tagggctcta
    16321 gaaagagctg ttaagctatc gaggttaggc tctgttacag aattttccca tcttgctact
    16381 gcctgttgtg tagagaagat tttttcagct aattcttttt gtgtccaccc gagagctttt
    16441 cgcttttctc taatgacctt tgcatattgt ttctgtttca ttaatacacc cccatctcag
    16501 tatagtacaa caagttgttg tatttgtaat ggtatttgaa gttttttata ttttatgagt
    16561 tgaaatacat catattgttg tatataataa aacaggaggg gagaatcatg aaaaaagtaa
    16621 gtaatctcag ccgatacaga aaagaaaaag gactttctca aacggagtta gcaaaaaaaa
    16681 tgaatgttac acagcaatgt gtaagttcgt ggcaaacagg gagaacgatt cctaaaccct
    16741 atcaaatgaa gatgctttct gagatattaa gtgtaccaat gaatgagctg ttttctgatg
    16801 tatttaataa agtaaacagt taactaataa gcaattgaat gattttagga ggagaagaca
    16861 tgtattgtag aaaatgtggc aaagaaataa cagatgaaag taagtattgc cagttttgtg
    16921 gagcactaca agaaacagat gagacaaccg gaacagacac agtaaagaaa agtactgagc
    16981 agacaattaa tgaaatgaag gaacaaatcg tacatggaca agagacaggg aagctgtatt
    17041 tgattattgg ttggatttct atggtagtat cgttagcatt tattcctgtt gtatttggtg
    17101 ctgttgctgt tattatgggc tacttatatc gagaaaaaga tgagaaaatg ggtacgattt
    17161 tgatgatagc aggggtagct ggagcaatct ttggtgtact tttagggatg tcaatgtact
    17221 gataagaggg ataagaacaa attattttgg agataggaga taaaaagttg aataaagtaa
    17281 atatgaacaa gaagtttgtc attataggta ttgttgtctt ggctattata ggtattattt
    17341 tttcaaaaat gacaggtaca tctggattag agaagtcatc aaaaccattg gtaacacaga
    17401 ttttacaaga acagtacaac ttaaatcgat catgtgatga tgtgacgatt actcatgaaa
    17461 atggagataa cacataccgg gctaaagcta tattagacaa tggaagtgct atcaacataa
    17521 atattgagta ctatccaaag aaagaccgta tatatgtgga gataccatat gctgaggtgt
    17581 taatgttaaa ttgatttaaa tgtattatgc ataccgtcca aacaggaact cccaaaggtg
    17641 taaattcact agggagtttt tatagtttcc tataaattaa atactaatac aggtgacaaa
    17701 aaaatattac aatgattgtc gggaa
//
LOCUS       DAJSNV010000054        17369 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSNV010000054 DAJSNV010000000
VERSION     DAJSNV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.056"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(68..613)
                     /locus_tag="ON516_001765"
     CDS             complement(68..613)
                     /locus_tag="ON516_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3345245.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(625..1317)
                     /locus_tag="ON516_001766"
     CDS             complement(625..1317)
                     /locus_tag="ON516_001766"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3345246.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1336..1620)
                     /locus_tag="ON516_001767"
     CDS             complement(1336..1620)
                     /locus_tag="ON516_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345247.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1633..2196)
                     /locus_tag="ON516_001768"
     CDS             complement(1633..2196)
                     /locus_tag="ON516_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3345248.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2424..3086
                     /locus_tag="ON516_001769"
     CDS             2424..3086
                     /locus_tag="ON516_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3345249.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3210..4406
                     /locus_tag="ON516_001770"
     CDS             3210..4406
                     /locus_tag="ON516_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3345250.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4507..5883)
                     /locus_tag="ON516_001771"
     CDS             complement(4507..5883)
                     /locus_tag="ON516_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3345251.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5920..6786)
                     /locus_tag="ON516_001772"
     CDS             complement(5920..6786)
                     /locus_tag="ON516_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3345252.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6875..7900)
                     /locus_tag="ON516_001773"
     CDS             complement(6875..7900)
                     /locus_tag="ON516_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3345253.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7918..8592)
                     /locus_tag="ON516_001774"
     CDS             complement(7918..8592)
                     /locus_tag="ON516_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345254.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8776..9669)
                     /locus_tag="ON516_001775"
     CDS             complement(8776..9669)
                     /locus_tag="ON516_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3345255.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9743..11440)
                     /locus_tag="ON516_001776"
     CDS             complement(9743..11440)
                     /locus_tag="ON516_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3345256.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11433..12746)
                     /locus_tag="ON516_001777"
     CDS             complement(11433..12746)
                     /locus_tag="ON516_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3345257.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12746..17254)
                     /locus_tag="ON516_001778"
     CDS             complement(12746..17254)
                     /locus_tag="ON516_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3345258.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
BASE COUNT         4860 a         3901 c         3015 g         5593 t
ORIGIN      
        1 agtcgtttgt tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt
       61 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
      121 gtccaacggg atcgaaactt gccacggcat ctttgaaaat atggagtttg tatcctagat
      181 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
      241 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
      301 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
      361 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
      421 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
      481 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
      541 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
      601 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
      661 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
      721 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
      781 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
      841 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
      901 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
      961 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
     1021 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     1081 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     1141 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     1201 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     1261 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     1321 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     1381 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     1441 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     1501 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     1561 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     1621 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     1681 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     1741 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     1801 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     1861 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     1921 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     1981 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     2041 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     2101 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     2161 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     2221 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     2281 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     2341 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     2401 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     2461 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     2521 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     2581 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     2641 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     2701 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     2761 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     2821 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     2881 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     2941 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     3001 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     3061 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     3121 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     3181 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     3241 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     3301 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     3361 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     3421 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     3481 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     3541 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     3601 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     3661 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     3721 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     3781 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     3841 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     3901 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     3961 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     4021 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     4081 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     4141 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     4201 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     4261 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     4321 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     4381 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     4441 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     4501 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     4561 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     4621 tacaatacaa gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     4681 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     4741 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     4801 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     4861 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     4921 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     4981 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     5041 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     5101 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     5161 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     5221 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     5281 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     5341 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     5401 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     5461 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     5521 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     5581 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     5641 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     5701 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     5761 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     5821 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     5881 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     5941 taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt
     6001 tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat
     6061 ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat
     6121 gtttcctaag ccccataata atccacagat catgtttttc cacacaaact gatcgacttt
     6181 aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat
     6241 aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat
     6301 cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc
     6361 tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa
     6421 agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc
     6481 cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc
     6541 catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa
     6601 aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa
     6661 agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat
     6721 tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat
     6781 ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac
     6841 aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta
     6901 agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat
     6961 ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg
     7021 ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt
     7081 ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt
     7141 ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt
     7201 ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga
     7261 tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa
     7321 tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg
     7381 cataatacaa aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg
     7441 tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa
     7501 gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac
     7561 tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta
     7621 ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt
     7681 gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa
     7741 aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga
     7801 tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga
     7861 tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca
     7921 gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc
     7981 gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc
     8041 gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat
     8101 caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc
     8161 catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa
     8221 ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat
     8281 ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat
     8341 attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg
     8401 acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc
     8461 ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg
     8521 ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat
     8581 gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc
     8641 cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg
     8701 gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc
     8761 ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg
     8821 ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa
     8881 tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg
     8941 aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat
     9001 aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac
     9061 aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg
     9121 gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg
     9181 ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac
     9241 tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt
     9301 gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt
     9361 ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac
     9421 tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt
     9481 ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata
     9541 attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat
     9601 cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt
     9661 ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat
     9721 tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc
     9781 tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac
     9841 cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta
     9901 ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg
     9961 ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg
    10021 ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg
    10081 cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg
    10141 actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg
    10201 catctttaag caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg
    10261 tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt
    10321 caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat
    10381 tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt
    10441 tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta
    10501 ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat
    10561 cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc
    10621 ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag
    10681 ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa
    10741 taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga
    10801 tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat
    10861 cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc attggagacg
    10921 cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag
    10981 aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg
    11041 ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt
    11101 tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag
    11161 gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc
    11221 ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg
    11281 gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga
    11341 cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta
    11401 acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt
    11461 tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt
    11521 gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca
    11581 ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt
    11641 gcggattcat aggcagagac tggaactttc ccggattcta attcatccat ctcctgtccg
    11701 ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc
    11761 tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa
    11821 taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa
    11881 atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga
    11941 tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc tatcatctga
    12001 tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt
    12061 ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc
    12121 tcttgcagtt cctcgcttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg
    12181 atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa
    12241 atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg
    12301 tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc
    12361 ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg
    12421 aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg
    12481 taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc
    12541 cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa
    12601 tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct
    12661 tctgccgaca tcgcctgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga
    12721 agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa
    12781 acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata
    12841 atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct
    12901 gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg
    12961 tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc
    13021 ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa
    13081 agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc
    13141 cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc
    13201 cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt
    13261 caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt
    13321 catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag
    13381 cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa
    13441 actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat
    13501 attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc
    13561 ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta
    13621 acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat
    13681 cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc
    13741 gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg
    13801 tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag
    13861 cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg
    13921 ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat
    13981 aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat
    14041 atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt
    14101 tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt
    14161 cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga
    14221 tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat
    14281 actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat
    14341 aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat
    14401 ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga
    14461 attgatacgt ctcatatact aactgataac gtggtggtcc agcgatctct ctgatccaaa
    14521 atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg
    14581 ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat
    14641 tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc
    14701 tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc
    14761 gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca
    14821 ctgtgtaaaa atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga
    14881 ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga
    14941 catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc
    15001 atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa
    15061 tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg
    15121 tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt
    15181 cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa
    15241 tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat
    15301 cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc
    15361 aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat
    15421 tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt
    15481 ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg
    15541 cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat
    15601 ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc
    15661 aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg
    15721 tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt
    15781 tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg
    15841 tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa
    15901 agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca
    15961 ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta
    16021 ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt aattcactca
    16081 tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc
    16141 ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag
    16201 atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat
    16261 ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa
    16321 cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc
    16381 agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg
    16441 agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat
    16501 agtcattcgt tgtaaatttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat
    16561 agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga
    16621 ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct
    16681 tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat
    16741 tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttccc
    16801 cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt
    16861 ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt
    16921 ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc
    16981 cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt
    17041 caaaagccag atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg
    17101 catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat
    17161 ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta
    17221 ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt
    17281 tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt
    17341 taattttaaa tcaaatgata tccttttta
//
LOCUS       DAJSNV010000055        16529 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSNV010000055 DAJSNV010000000
VERSION     DAJSNV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.057"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(36..401)
                     /locus_tag="ON516_001779"
     CDS             complement(36..401)
                     /locus_tag="ON516_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345259.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            609..1091
                     /locus_tag="ON516_001780"
     CDS             609..1091
                     /locus_tag="ON516_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345260.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1288..2178
                     /locus_tag="ON516_001781"
     CDS             1288..2178
                     /locus_tag="ON516_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3345261.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2503..4974
                     /locus_tag="ON516_001782"
     CDS             2503..4974
                     /locus_tag="ON516_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3345262.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5077..6813)
                     /locus_tag="ON516_001783"
     CDS             complement(5077..6813)
                     /locus_tag="ON516_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3345263.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6810..7514)
                     /locus_tag="ON516_001784"
     CDS             complement(6810..7514)
                     /locus_tag="ON516_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345264.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7645..8148
                     /locus_tag="ON516_001785"
     CDS             7645..8148
                     /locus_tag="ON516_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3345265.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8108..8446
                     /locus_tag="ON516_001786"
     CDS             8108..8446
                     /locus_tag="ON516_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3345266.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8467..9885
                     /gene="ffh"
                     /locus_tag="ON516_001787"
     CDS             8467..9885
                     /gene="ffh"
                     /locus_tag="ON516_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3345267.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9997..10575
                     /locus_tag="ON516_001788"
     CDS             9997..10575
                     /locus_tag="ON516_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3345268.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10579..11100
                     /locus_tag="ON516_001789"
     CDS             10579..11100
                     /locus_tag="ON516_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3345269.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11179..11733)
                     /locus_tag="ON516_001790"
     CDS             complement(11179..11733)
                     /locus_tag="ON516_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3345270.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11986..12261
                     /gene="rpsP"
                     /locus_tag="ON516_001791"
     CDS             11986..12261
                     /gene="rpsP"
                     /locus_tag="ON516_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3345271.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12273..12524
                     /locus_tag="ON516_001792"
     CDS             12273..12524
                     /locus_tag="ON516_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3345272.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12649..13440
                     /locus_tag="ON516_001793"
     CDS             12649..13440
                     /locus_tag="ON516_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345273.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13610..14131
                     /gene="rimM"
                     /locus_tag="ON516_001794"
     CDS             13610..14131
                     /gene="rimM"
                     /locus_tag="ON516_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3345274.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14121..14882
                     /gene="trmD"
                     /locus_tag="ON516_001795"
     CDS             14121..14882
                     /gene="trmD"
                     /locus_tag="ON516_001795"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3345275.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15018..15365
                     /gene="rplS"
                     /locus_tag="ON516_001796"
     CDS             15018..15365
                     /gene="rplS"
                     /locus_tag="ON516_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3345276.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15623..15916
                     /locus_tag="ON516_001797"
     CDS             15623..15916
                     /locus_tag="ON516_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3345277.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5536 a         2955 c         3369 g         4669 t
ORIGIN      
        1 aaagtagctt ctctttgtac atatatggtt tgtttttatg gatgccgagc ttttttataa
       61 gataaggcta ccgcccataa cgacccaagg atttgtggaa ctaagtaaat acctgcaaga
      121 agcaagatca gctgggtcgg ttttaaagga tgatagaagt aacgccattc agtaaaattg
      181 gcgcaaaata aataggaagt gacagtagat ataatattgg caatgaataa agatataata
      241 cgcctgttcc tttgaaaata atatccagca aaaagagata aaaatatcaa tgtgatgaac
      301 ccgcttacgg aatgataacg aaaatcaagc agtaaggcga aatgcacata aggaacaaaa
      361 gataaaagca acggaaggtt tttccagcaa gtttttatca tctttcatca ccacctaaca
      421 agtgttatta ttttgttagt tttattgtaa attgaatcct gcaataatca attacttttt
      481 cattatttct agattaaatg aataaactct catgccatta tctctaaaaa aaaagttttt
      541 ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag tagtttgggg
      601 gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt tggttttttc
      661 ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg gagatattag
      721 gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg actgttttct
      781 atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac tacaattggc
      841 agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg ctgcctcgct
      901 tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggattcagct tattcctcca
      961 tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt attcttgcaa
     1021 cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta tctataaaaa
     1081 ataactattg atggtggatc atcagtaaaa aagcatatga gctgcgtaaa atgcatgttt
     1141 ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat gatttttaca
     1201 taacttaact atatttttac agcaaccata cgagggattt acagtcgact gataaacttt
     1261 agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct aggtatgatg
     1321 ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc tgccacacaa
     1381 aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc gacagcatta
     1441 cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc
     1501 tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg agcagttact
     1561 atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc aaaattagtg
     1621 gatcataaag tagcagtggt cgggatggcg ccaatcgtca ataaagatac agatgtcaaa
     1681 gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa ctggaaggaa
     1741 gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc
     1801 gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca agaacaagat
     1861 tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag ctatttagct
     1921 ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga accaaaagaa
     1981 gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta cacgaacgga
     2041 aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga aatccaagaa
     2101 ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga acgtgatcac
     2161 gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac tattaaggaa
     2221 aaatcaacta tgtttcccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     2281 attttgccgt tttttgagct aataccgagg gtcagctgat taaacaccgt ttattcgaaa
     2341 aaaagagcta cgacatagaa ctgtcgtagc tctttttttg ctatatgaca ggaatgaaag
     2401 gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg gataggagaa
     2461 gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa taaatggata
     2521 aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat ccgtcttttt
     2581 ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga aaaaggtgca
     2641 gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt ggaaaacata
     2701 tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt agtagaatcc
     2761 tataagaact tgactttttt accagttatc atagccaata caggaatcgc catttttcag
     2821 gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc aaaagtcaat
     2881 gtcatacggg acgggataga acaaacaata gatatagaag accttgtaat agatgacatt
     2941 gttattttag agactggaca tcaaattcca gcagatgcag aggtcgtgga aggaaaactg
     3001 caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga aataggcgat
     3061 ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt agacaaagtt
     3121 ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg agaaggagaa
     3181 cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat catcattatt
     3241 cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac gatcaaagaa
     3301 agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact gtacttgctg
     3361 acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt tcttttacac
     3421 aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga taaaacagga
     3481 acgatcacag aaaataaaat gtctgttcaa aaagtgatcg tctctaaaaa acaagaaata
     3541 atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc gatggcagga
     3601 gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga tcaatcctat
     3661 caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag
     3721 gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa
     3781 gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg
     3841 attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat tctgattgct
     3901 aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca gcaagtagcc
     3961 atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt ccaagcaggg
     4021 atttcacatg ctgatcagta cgttgatgca tctaaattat cagaagaaga atatgaagca
     4081 gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt
     4141 caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta
     4201 gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag
     4261 gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa
     4321 ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt
     4381 ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca
     4441 tcacaaatca cattgattag tttgtttact atcggacttc catctttctt gcttgctcta
     4501 gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg
     4561 ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg
     4621 aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc
     4681 ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc
     4741 tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt
     4801 atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt
     4861 aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag
     4921 ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa
     4981 gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt
     5041 catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt
     5101 gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa ggagttgcac
     5161 gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt
     5221 gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc
     5281 aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct
     5341 gtcgcttttt gaatattgga cagcattttc aatcagattt ttgataattg gataaaggat
     5401 atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt
     5461 ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt
     5521 ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt
     5581 atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc
     5641 atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt
     5701 taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt
     5761 cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg
     5821 caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg
     5881 atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt
     5941 gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag
     6001 cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag
     6061 taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac tcatttgttc
     6121 gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac
     6181 ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg
     6241 ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga agaaagacag
     6301 aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg
     6361 ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata
     6421 taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt
     6481 caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt
     6541 gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac
     6601 gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg
     6661 gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg
     6721 aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa
     6781 gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa cttatagcca
     6841 aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat tttttctcgc
     6901 aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg
     6961 cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt
     7021 aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg
     7081 gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat
     7141 tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac ttcgcgagga
     7201 ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact
     7261 tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc
     7321 tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat
     7381 gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag
     7441 ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact
     7501 agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata
     7561 gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg
     7621 ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc
     7681 tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga
     7741 gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct
     7801 gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca
     7861 agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg
     7921 caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat
     7981 tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct
     8041 tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt
     8101 gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac
     8161 cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt
     8221 aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg
     8281 gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt
     8341 tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa
     8401 aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa
     8461 gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga
     8521 aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc
     8581 ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa
     8641 cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc
     8701 gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca
     8761 aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact
     8821 ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg
     8881 gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt
     8941 cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag
     9001 ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc
     9061 gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt
     9121 ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac
     9181 cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg
     9241 gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa
     9301 aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg
     9361 ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa
     9421 gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt
     9481 gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat
     9541 caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg
     9601 atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt
     9661 cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa
     9721 caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca
     9781 ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat
     9841 cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc
     9901 ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt
     9961 actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa
    10021 acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa
    10081 aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt
    10141 ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca
    10201 aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga
    10261 agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc
    10321 ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat
    10381 cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta
    10441 aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc
    10501 gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc
    10561 agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc
    10621 tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa
    10681 actacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat
    10741 caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt
    10801 ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt
    10861 gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt
    10921 cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc
    10981 cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt
    11041 gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag
    11101 aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt
    11161 ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa
    11221 aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat caattagatt
    11281 cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag
    11341 tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac
    11401 gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa
    11461 agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc
    11521 gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt
    11581 ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc
    11641 ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa
    11701 actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta
    11761 taataatctt acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta
    11821 catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa
    11881 aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg
    11941 ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc
    12001 cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca
    12061 cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac
    12121 cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa
    12181 ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa
    12241 gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa
    12301 caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga
    12361 aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa
    12421 aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac
    12481 cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact
    12541 cgaattttcg agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca
    12601 aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa
    12661 atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca
    12721 gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt
    12781 accaaaacgg cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact
    12841 gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc
    12901 tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat
    12961 tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt
    13021 gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag
    13081 ccatttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat
    13141 cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga
    13201 atccgtggga ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct
    13261 tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg
    13321 ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg
    13381 atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga
    13441 aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat
    13501 agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact agaattgcct
    13561 cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata
    13621 cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc
    13681 aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga
    13741 cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt
    13801 attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt
    13861 aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat
    13921 catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc
    13981 accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc
    14041 atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat
    14101 tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg
    14161 aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca
    14221 atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat gattatccat
    14281 acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca
    14341 ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac
    14401 gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg
    14461 gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag
    14521 gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc
    14581 ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg
    14641 gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag
    14701 aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa
    14761 gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga
    14821 cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt
    14881 gactttttct aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt
    14941 gcaaaagcac ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga
    15001 ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt
    15061 ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag
    15121 gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa
    15181 tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc
    15241 cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg
    15301 caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc
    15361 gttaatcatt gttatcagac tgagaaagaa cccttggtat tcaagggttc ttttttctta
    15421 gcatgtaatt tgaacaccct gatccagttt tttctttaat tattggtagg ggagaataat
    15481 ggattagaat aaatctacct ataagtagta gtaaaaaaaa gagcactatg ataatatctt
    15541 tcttaagagt aaccgcttta ataaaaaatc cagaatattc taacataaat ggagtattct
    15601 tagaatcaaa aggagaaaga agatggttta tacttatacg gagcaagaaa agatgaaagt
    15661 tttagacaca ttcatgaatg atggaaaagt aaaacaagta ccaagttcaa caaaaaagaa
    15721 atacatccta ttgaaagaag tcttgaaacg ctttgaccat ggagtgactt atacagaaac
    15781 agaggttaat tcgatactgc tgaacgtatt ttctagtggc gattatgtcg aacaaagacg
    15841 ttatctaatc acatttggct ttttcaaaag aagttcagac ggtcgggcat atcagatgat
    15901 gggaattgaa aattagaagt tttaatttat actcattgaa aaaagttcaa gtatgcgata
    15961 ttggttctac accgcatact tgaacttttt ttgtcatctg tatctataat acaaagccaa
    16021 gaacgttatt caaaatatgg atagctatga taaaagctaa tgagcgattc gcagttaaat
    16081 aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa aaaatcaatt ggatgcgtta
    16141 catgaataaa gataattctc ttttatttgt acgaataaat accaaaacgt acctcctaaa
    16201 attagatttt ggacagatgc cctttaaaaa gataacaaga tttcgttctt gaaacaatat
    16261 atatgctttt attccaatga ggttgtgaaa acagtatttt gtcctgagcg ttagaaaatt
    16321 gaaaatgggg aatagcaact cgtactttac cattttttaa gtgaatacaa gatcagctaa
    16381 ttgaacaccg tttattcgtg atgagggaca caacactgca gtcgacacat ttctctttta
    16441 tgttcaaaga taacaaatat ttaaaacaaa gaaaaatagt agataatccc gtcctaactc
    16501 ttttattctt ccctcatagc ttccaaatt
//
LOCUS       DAJSNV010000056        16354 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSNV010000056 DAJSNV010000000
VERSION     DAJSNV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.058"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..258)
                     /locus_tag="ON516_001798"
     CDS             complement(<1..258)
                     /locus_tag="ON516_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345278.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ON516_001799"
     CDS             complement(274..924)
                     /locus_tag="ON516_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345279.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ON516_001800"
     CDS             complement(935..2107)
                     /locus_tag="ON516_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR3345280.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ON516_001801"
     CDS             complement(2126..2998)
                     /locus_tag="ON516_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3345281.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ON516_001802"
     CDS             complement(3020..3940)
                     /locus_tag="ON516_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345282.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ON516_001803"
     CDS             complement(3940..4209)
                     /locus_tag="ON516_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345283.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ON516_001804"
     CDS             complement(4206..4586)
                     /locus_tag="ON516_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3345284.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ON516_001805"
     CDS             complement(5053..6516)
                     /locus_tag="ON516_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3345285.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ON516_001806"
     CDS             complement(6580..9597)
                     /locus_tag="ON516_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3345286.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ON516_001807"
     CDS             complement(9748..10587)
                     /locus_tag="ON516_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3345287.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ON516_001808"
     CDS             10732..10977
                     /locus_tag="ON516_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345288.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ON516_001809"
     CDS             11040..11462
                     /locus_tag="ON516_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345289.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ON516_001810"
     CDS             complement(11556..11873)
                     /locus_tag="ON516_001810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345290.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ON516_001811"
     CDS             complement(11900..12106)
                     /locus_tag="ON516_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345291.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ON516_001812"
     CDS             complement(12087..12269)
                     /locus_tag="ON516_001812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345292.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ON516_001813"
     CDS             complement(12283..12633)
                     /locus_tag="ON516_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345293.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ON516_001814"
     CDS             complement(12659..12961)
                     /locus_tag="ON516_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345294.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ON516_001815"
     CDS             complement(12964..13197)
                     /locus_tag="ON516_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345295.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="ON516_001816"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="ON516_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="ON516_001817"
     CDS             complement(14628..15743)
                     /locus_tag="ON516_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3345296.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ON516_001818"
     CDS             complement(15749..16147)
                     /locus_tag="ON516_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3345297.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4331 a         3422 c         2564 g         6037 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttctttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctat
//
LOCUS       DAJSNV010000057        16190 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSNV010000057 DAJSNV010000000
VERSION     DAJSNV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.059"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(238..1965)
                     /locus_tag="ON516_001819"
     CDS             complement(238..1965)
                     /locus_tag="ON516_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3345298.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1958..3151)
                     /locus_tag="ON516_001820"
     CDS             complement(1958..3151)
                     /locus_tag="ON516_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3345299.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3338..3982)
                     /locus_tag="ON516_001821"
     CDS             complement(3338..3982)
                     /locus_tag="ON516_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3345300.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4076..4210)
                     /locus_tag="ON516_001822"
     CDS             complement(4076..4210)
                     /locus_tag="ON516_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3345301.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4212..6344)
                     /gene="feoB"
                     /locus_tag="ON516_001823"
     CDS             complement(4212..6344)
                     /gene="feoB"
                     /locus_tag="ON516_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3345302.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6341..6814)
                     /locus_tag="ON516_001824"
     CDS             complement(6341..6814)
                     /locus_tag="ON516_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3345303.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7215..7439
                     /gene="nrdH"
                     /locus_tag="ON516_001825"
     CDS             7215..7439
                     /gene="nrdH"
                     /locus_tag="ON516_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3345304.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7598..9757
                     /gene="nrdE"
                     /locus_tag="ON516_001826"
     CDS             7598..9757
                     /gene="nrdE"
                     /locus_tag="ON516_001826"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3345305.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9807..10772
                     /gene="nrdF"
                     /locus_tag="ON516_001827"
     CDS             9807..10772
                     /gene="nrdF"
                     /locus_tag="ON516_001827"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3345306.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10861..12000)
                     /locus_tag="ON516_001828"
     CDS             complement(10861..12000)
                     /locus_tag="ON516_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3345307.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12309..13022)
                     /locus_tag="ON516_001829"
     CDS             complement(12309..13022)
                     /locus_tag="ON516_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3345308.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13276..13977)
                     /gene="nagB"
                     /locus_tag="ON516_001830"
     CDS             complement(13276..13977)
                     /gene="nagB"
                     /locus_tag="ON516_001830"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3345309.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14211..15743
                     /locus_tag="ON516_001831"
     CDS             14211..15743
                     /locus_tag="ON516_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3345310.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15934..16113)
                     /locus_tag="ON516_001832"
                     /pseudo
     CDS             complement(15934..16113)
                     /locus_tag="ON516_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5009 a         3352 c         2839 g         4990 t
ORIGIN      
        1 tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg
       61 atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg aacgtttcga
      121 agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt
      181 ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta
      241 ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg
      301 tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg
      361 gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat
      421 cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc
      481 atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt
      541 gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc
      601 tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg
      661 ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc
      721 catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc
      781 gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac
      841 catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt
      901 catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac
      961 ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa
     1021 actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg
     1081 acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa
     1141 atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa
     1201 tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc
     1261 accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc
     1321 tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt
     1381 gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt
     1441 cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat
     1501 cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat
     1561 aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc
     1621 taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt
     1681 catcaaatca ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa
     1741 cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa
     1801 caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat
     1861 cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc
     1921 tacccagttt gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc
     1981 ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt aattttccat
     2041 cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca
     2101 ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt
     2161 gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt
     2221 caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc
     2281 catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt
     2341 tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata
     2401 tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc
     2461 caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg
     2521 cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga
     2581 acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc
     2641 gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag
     2701 aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt
     2761 ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga
     2821 aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata
     2881 catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac
     2941 ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt
     3001 ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg
     3061 ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct
     3121 ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat
     3181 ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa
     3241 tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg
     3301 tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg
     3361 ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt
     3421 cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa
     3481 ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca
     3541 gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta
     3601 ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt
     3661 gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt
     3721 gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat
     3781 gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag
     3841 gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat
     3901 atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga
     3961 cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta
     4021 ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc
     4081 gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg
     4141 attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga
     4201 atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat
     4261 tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc
     4321 cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact
     4381 aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt
     4441 tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac
     4501 aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca
     4561 ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta
     4621 gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga
     4681 aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt
     4741 tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac aaagataatc
     4801 gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac
     4861 cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac
     4921 aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag
     4981 gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc
     5041 ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc
     5101 tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc
     5161 ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa
     5221 gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc
     5281 aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc
     5341 tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac
     5401 catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata
     5461 gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc
     5521 gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca
     5581 cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta
     5641 tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa
     5701 actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac
     5761 cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct
     5821 aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga
     5881 atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt
     5941 acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca
     6001 tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa
     6061 taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc
     6121 tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg
     6181 ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct
     6241 actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag
     6301 ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt
     6361 gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca
     6421 gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca
     6481 atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc
     6541 cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt
     6601 tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca
     6661 gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc
     6721 cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa
     6781 cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat
     6841 catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga
     6901 gaatcattct cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt
     6961 atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt
     7021 ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt
     7081 ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt
     7141 tgtttgactt ttttactaag agttttatac tatctattat agataataag aatgtgttag
     7201 aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga
     7261 tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac
     7321 agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt
     7381 ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac
     7441 atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt
     7501 ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca
     7561 agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga
     7621 tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt
     7681 gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat
     7741 gacttttcca tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc
     7801 agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga
     7861 gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt
     7921 gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc
     7981 gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca
     8041 tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga
     8101 attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat
     8161 caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct
     8221 tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt
     8281 catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc
     8341 tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa
     8401 agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga
     8461 taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt
     8521 cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct
     8581 agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat
     8641 ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg
     8701 cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt
     8761 tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cagatatctc
     8821 atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc
     8881 tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt
     8941 accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt
     9001 acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac
     9061 aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg
     9121 tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt
     9181 cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga
     9241 aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga
     9301 tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa
     9361 cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa
     9421 aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac
     9481 ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaacgg cacaacaaca
     9541 cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta
     9601 tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc
     9661 tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga
     9721 aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt
     9781 catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga
     9841 atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc
     9901 tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg
     9961 aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg
    10021 aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga
    10081 acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac
    10141 tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc
    10201 aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa
    10261 ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt
    10321 atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg
    10381 aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg
    10441 aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa
    10501 acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga
    10561 cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc
    10621 cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca
    10681 accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga
    10741 aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg
    10801 aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc
    10861 ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat
    10921 tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac
    10981 aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa
    11041 gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt
    11101 tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga
    11161 gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat
    11221 aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct
    11281 cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata
    11341 ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt
    11401 cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa
    11461 aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga
    11521 gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc
    11581 tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt
    11641 taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg
    11701 ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa
    11761 aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat
    11821 tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa ttgccgaaag
    11881 cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat
    11941 cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat
    12001 acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct
    12061 gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag
    12121 tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc
    12181 aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata
    12241 aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct
    12301 cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac
    12361 aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt
    12421 ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata
    12481 tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg
    12541 atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata
    12601 gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct
    12661 aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg
    12721 acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt
    12781 gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca
    12841 ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata
    12901 tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca
    12961 aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc
    13021 atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa
    13081 tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa
    13141 taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag
    13201 ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta
    13261 gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt
    13321 gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc
    13381 tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat
    13441 aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa
    13501 tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa
    13561 tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat
    13621 atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc
    13681 tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc
    13741 catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt
    13801 tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac
    13861 tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat
    13921 gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt
    13981 gattgctcct ttatattttg gtatagccca attatagcat tctttttttc tttttacaat
    14041 aattagtata gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt
    14101 taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat
    14161 tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac
    14221 tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc
    14281 tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta
    14341 cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa
    14401 aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg
    14461 aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc
    14521 tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc
    14581 aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca
    14641 gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac
    14701 aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag
    14761 atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg
    14821 attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca
    14881 cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag
    14941 cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa
    15001 aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga
    15061 tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag
    15121 caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa
    15181 caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc
    15241 gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa
    15301 aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa
    15361 aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat
    15421 atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag
    15481 cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa
    15541 ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac
    15601 cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc
    15661 caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag
    15721 tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac
    15781 aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt
    15841 attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt
    15901 gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt
    15961 tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac
    16021 cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc
    16081 acctccaata aagtacataa acataaatac agacccgtgg tgctagttaa ttttaatatc
    16141 ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
//
LOCUS       DAJSNV010000058        16061 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSNV010000058 DAJSNV010000000
VERSION     DAJSNV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.060"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(119..421)
                     /locus_tag="ON516_001833"
     CDS             complement(119..421)
                     /locus_tag="ON516_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345311.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(641..1007)
                     /gene="ssrA"
                     /locus_tag="ON516_001834"
     tmRNA           complement(641..1007)
                     /gene="ssrA"
                     /locus_tag="ON516_001834"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1178..1951)
                     /locus_tag="ON516_001835"
     CDS             complement(1178..1951)
                     /locus_tag="ON516_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3345312.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2095..2445
                     /locus_tag="ON516_001836"
     CDS             2095..2445
                     /locus_tag="ON516_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3345313.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2426..3142
                     /locus_tag="ON516_001837"
     CDS             2426..3142
                     /locus_tag="ON516_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345314.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3142..4590
                     /locus_tag="ON516_001838"
     CDS             3142..4590
                     /locus_tag="ON516_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3345315.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4660..5169)
                     /locus_tag="ON516_001839"
     CDS             complement(4660..5169)
                     /locus_tag="ON516_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345316.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5159..5884)
                     /locus_tag="ON516_001840"
     CDS             complement(5159..5884)
                     /locus_tag="ON516_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345317.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6107..8227)
                     /locus_tag="ON516_001841"
     CDS             complement(6107..8227)
                     /locus_tag="ON516_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3345318.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8457..9635
                     /locus_tag="ON516_001842"
     CDS             8457..9635
                     /locus_tag="ON516_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3345319.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9651..10409
                     /gene="aroD"
                     /locus_tag="ON516_001843"
     CDS             9651..10409
                     /gene="aroD"
                     /locus_tag="ON516_001843"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3345320.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10432..10917
                     /gene="ybaK"
                     /locus_tag="ON516_001844"
     CDS             10432..10917
                     /gene="ybaK"
                     /locus_tag="ON516_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3345321.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10988..12241)
                     /locus_tag="ON516_001845"
     CDS             complement(10988..12241)
                     /locus_tag="ON516_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3345322.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12358..13707
                     /gene="gdhA"
                     /locus_tag="ON516_001846"
     CDS             12358..13707
                     /gene="gdhA"
                     /locus_tag="ON516_001846"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3345323.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13819..15165
                     /locus_tag="ON516_001847"
     CDS             13819..15165
                     /locus_tag="ON516_001847"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3345324.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15473..15859)
                     /locus_tag="ON516_001848"
     CDS             complement(15473..15859)
                     /locus_tag="ON516_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3345325.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15908..>16061)
                     /locus_tag="ON516_001849"
     CDS             complement(15908..>16061)
                     /locus_tag="ON516_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3345326.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT         5259 a         2875 c         3207 g         4720 t
ORIGIN      
        1 atactataaa atatagatat catcatatta cacaatctta atactaactt aaaaaatatc
       61 ccttttataa gtatggtgat aaaatccgtt ccgggctacc ttttttaggt agcctacttt
      121 aatctttata cctttctgga tcaacgaaag tatacttcaa atagtcataa cgccgatgat
      181 cgctccgtgc gtctggcaca tcagtcacga tatcaaacaa aaaatacaca tctttcttca
      241 ttctggtttt tgttgccgga attttaaagt agttcttatt agagtaatag agattgatta
      301 ataagctatc ttcgattgct aaaaagaaaa cttctgaatc ccacacctta taaaaatctt
      361 tgacaaatct attcgaagga tcaaatttaa accataattg tgtttttcct tccatcaaca
      421 taatgttcac ctcaaaaaga gtatacgaac taatgttctt tttgtaaagt aggaatatca
      481 ttgataatat ggatggaaca tatatattaa aaagtgttct ttttgaatgc tattttgaat
      541 gccattttca ttcatttaac gaaaatctgc gaaaataaaa aatgctcaaa cccttataaa
      601 ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac ggcgggagtc
      661 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      721 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      781 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      841 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      901 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      961 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
     1021 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
     1081 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
     1141 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
     1201 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
     1261 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
     1321 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
     1381 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
     1441 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
     1501 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
     1561 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1621 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1681 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1741 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1801 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1861 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1921 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1981 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     2041 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     2101 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     2161 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     2221 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     2281 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     2341 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     2401 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     2461 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     2521 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     2581 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2641 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2701 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2761 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagtagt
     2821 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2881 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2941 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     3001 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     3061 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     3121 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttctgcgga
     3181 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     3241 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     3301 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     3361 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     3421 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     3481 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     3541 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3601 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3661 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3721 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3781 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3841 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3901 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3961 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     4021 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     4081 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     4141 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     4201 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     4261 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     4321 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     4381 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     4441 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     4501 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     4561 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4621 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4681 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4741 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4801 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4861 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4921 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4981 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     5041 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     5101 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     5161 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     5221 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     5281 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     5341 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     5401 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     5461 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     5521 taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc aattcctgct
     5581 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5641 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5701 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5761 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5821 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5881 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5941 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     6001 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     6061 gagcagcgac aataattttg tcgctgctcc ttttaaattt attttatcat tcggaagaac
     6121 ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt
     6181 gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt
     6241 ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac
     6301 tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt
     6361 tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa
     6421 tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa
     6481 caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc
     6541 ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat
     6601 caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca
     6661 tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc
     6721 caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa
     6781 ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta
     6841 cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat
     6901 tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata
     6961 cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt
     7021 ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat
     7081 aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga
     7141 atgtaccagc gttcccatgg aataccgcac taatgtaacc ttgtgtttga cttaaaatat
     7201 caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca
     7261 agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt
     7321 gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag
     7381 gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct
     7441 ggaagctttc taaatggata taaatcacat tttttcctct agcgagcccg aacaaatcgt
     7501 catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac
     7561 ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag
     7621 cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg
     7681 gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga
     7741 tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca
     7801 gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc
     7861 gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg
     7921 taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa
     7981 gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa
     8041 ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc
     8101 cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta
     8161 aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac
     8221 atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt
     8281 acacagctca cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc
     8341 agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag
     8401 acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga
     8461 aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa
     8521 aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc
     8581 aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat
     8641 tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa
     8701 aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg
     8761 gagaaggaga cgggatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt
     8821 cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa
     8881 tctacccgga aatcatagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg
     8941 agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag
     9001 taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag
     9061 aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta
     9121 gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg
     9181 tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg
     9241 atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa
     9301 gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga
     9361 aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt
     9421 tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat
     9481 tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt
     9541 tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga
     9601 aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggttcggg
     9661 taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg
     9721 tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg
     9781 aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg
     9841 ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg
     9901 aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac
     9961 acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa
    10021 gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta
    10081 aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag
    10141 ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg
    10201 agttgactag acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa
    10261 atcttggtaa aatttctcgt ctggttggcg gaacgacggg ttccgttatg acatttggtt
    10321 ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa
    10381 ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag
    10441 aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca
    10501 gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt
    10561 ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct
    10621 cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca
    10681 gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata
    10741 cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt
    10801 gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta
    10861 gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc
    10921 atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt
    10981 ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg
    11041 ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca
    11101 gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc
    11161 cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt
    11221 tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat
    11281 ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag
    11341 cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt
    11401 gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta
    11461 attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg
    11521 tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta
    11581 aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg
    11641 atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga
    11701 tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga
    11761 taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt
    11821 tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac
    11881 gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat
    11941 gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat
    12001 tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat
    12061 catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt
    12121 ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga
    12181 tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca
    12241 ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt
    12301 gagccaaaag agaaaatgag ctaaaatgag ggaagataag caaaggagcg tggaacgatg
    12361 acaaacgcaa cagaatatgt tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg
    12421 caagaagaat ttttacaagc aatcgatgaa ttcatgccca cggttatgtc ttttttggac
    12481 actcatcccg aatatatcga aaagaatatc ttagaacttt taacggaacc agaaagagtt
    12541 attcaatttc gagtgccttg gcaagatgat gaaggaaact ggcgagtaaa tagagggtac
    12601 cgtatccaat acaactcagc tatcggccct tataaaggtg gattgcgctt ccatcctagt
    12661 gtcaatctaa gtatcttgaa atttttggct tttgaacaga ttttcaagaa tagtttaacc
    12721 ggtctgccaa tcggtggagg aaaaggcgga agtgacttcg atcctaaagg aaaatctgat
    12781 aatgagatca tgcgtttttg tcaaagtttc atgctggaat tgtcaaaaca tatcggccca
    12841 tcgattgatg ttcctgcggg agatatcggt gtaggagcta gagaaatcgg ctatttgttc
    12901 ggcgaatata aacgattgaa aaaatatgat actggtgtgt tgacaggtaa acctttggat
    12961 ttttggggaa gtaagatacg tacggaagct actggttatg gattagtgta ttatgtcaaa
    13021 catctattga acgaagaaaa agattcattt aatcaaaaaa cagtttttgt ttccggaagc
    13081 gggaatgtag ccatttatgc aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca
    13141 tgttcagatt ctagcgggta catctatgat ccagaaggaa tagatgtgaa actattgaaa
    13201 gaagtcaaag aaatcaatag acaacgtttg acggaatacg caaaccaacg tccttctgct
    13261 atttatcatg ctggagaatc tgtttggaca ttgaaagaaa aagcggatat cgctttgcca
    13321 tgtgctactc aaaatgaaat agacgaaagc ttggccgaaa tcttagtaga aaatgggatc
    13381 aaaatcgtag cagaaggcgc taatatgcca tgtacagtaa aagctgtttc tgttcttcat
    13441 gaagcaggcg tgtggtattg tccaggaaaa gctgctaatg ctggaggagt agcagtatct
    13501 gctttagaaa tgagtcagaa cgtccagcga ttgacttggg aaagagagca agtggatgag
    13561 cagctggatc aaatcatgga aacgatttac catacgtgtc gccaaacagc agccgagtac
    13621 ggcgacgaaa aaaatctgct tcttggagca aatgttgctg gatttgaaaa agttgccaaa
    13681 gcaatggcag cagaaggttt agtttgaaaa atgaaacttt cttggaaata ttgtcagcat
    13741 tttgtttatt tgcttgaaaa ataataaaaa aaaacgtaaa gtattacctg tagagtaaac
    13801 ctataaggag gagaacaaat ggcacacatt cattttgact attcaaaagt agcaccgttt
    13861 gtcaacgaac aagagttagg gtacatgcaa agccaagtaa cagcagccca caacgaatta
    13921 cgggaaggta ctggcgcagg aagcgacttc cgcggctgga tcgatttacc aacgaactat
    13981 gacaaagaag aatttgcacg tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa
    14041 gtattagttg ttatcggtat cggtggttct tatttaggcg ctcgtgcagc gatcgacttc
    14101 ttgaatcaca cattcttcaa tttattagat agcaacacaa gaaaagcacc acaaatcttc
    14161 tttgccggaa attcaatcag ctcaacatat attgctgact tgatcgaagt aatcggtgat
    14221 cgcgactttt cagtaaacgt tatttcaaaa tctggaacaa ctactgaacc agcgattgcg
    14281 ttccgtgtat tcaaagagct tcttgttaag aaatatggac aagaagaagc aaacaaacgt
    14341 atctacgcta caacagataa agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt
    14401 tgggaaactt tcgtgattcc tgatgacatc ggcggacgtt tctctgtatt aactgctgtt
    14461 ggtttgttac caatcgctgt tagtggtgca gatatcgatg cattgatgca aggtgccgct
    14521 gatgcaagca aagcatactc aagcgacaaa ctagaagaaa acgaagctta ccaatacgca
    14581 gcaatgcgca acattcttta ccgtaaaggc aaagtaactg agttgttgat caactatgaa
    14641 ccaggaatgc aatacttctc agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa
    14701 gaccaaaaag gcatctatcc ttcaagcgca aacttctcaa ctgatttgca ctctttagga
    14761 caattcatcc aagaaggacg tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct
    14821 cgtaaatcaa tcacaattcc tgaacaagca gaagatttag acggtctagg ctacttgcaa
    14881 ggaaaagaag tggacttcgt aaatacaaaa gcatttgaag gaacattgct tgctcataca
    14941 gacggtgacg tgcctaactt gctagtcaaa attccagaaa tggatgctta tacattgggt
    15001 tatacaatgt acttctttga aattgctgta ggtatttcag gttacttgaa tggaatcaac
    15061 ccattcgacc aaccaggtgt agaagcttac aagaaaaaca tgttcgcact tctaggcaaa
    15121 ccaggctttg aagatcttgc taaagaattg aacgaacgcc tgtaagataa gggtttttcg
    15181 ctagatttaa ataatcagtg atagaaacat gttcgcttta aaatgaaaac tggtacccat
    15241 ttaggtactc aaacatactg catcgtttat tcagaataag gggctgtgac aagacgtttg
    15301 tcacagtccc ttgtttgtat agaaatcgat tgattgcctt gctctttagt ttgttttagg
    15361 actttaagaa cagtgaggct gacttcgaag gttaattctg tttttttgtt ttctagaatc
    15421 agtaagcaaa aaagcactca cttcgagtgc ttttttgcga tatattctta gcttaagata
    15481 ggcctaattt ttcttttaca gatgagggta ccttatcgct ggtaattact tttttctcta
    15541 tgatccgctt ttcgcttgct ttcaactgca agatcgttcc ttcggggtaa gcagaaccat
    15601 tggcagagtc accagaagta atgacatcga aatctaactg tcttttttca ccgttttcag
    15661 cataagcatc tacgttgtat ttaaaacctc ttccaattac ttccccatta tctgctttga
    15721 tatcagtttc tgcaggcatt ggagctttga ctaccgcata ataatcttta cctacataag
    15781 tagactgata gtagtcccaa cctttccatc ctataaaagc aatcaatatg ataacgatca
    15841 accctaaaat ttttttcatg aaagatctcc ttttttattt ttattatacc cgtggtgcta
    15901 gttaatttta atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac
    15961 ttaatagaac actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag
    16021 aacgtgagtt gaaattttga caacctaatt tttctaagga a
//
LOCUS       DAJSNV010000059        16013 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSNV010000059 DAJSNV010000000
VERSION     DAJSNV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.061"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            226..894
                     /locus_tag="ON516_001850"
     CDS             226..894
                     /locus_tag="ON516_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3345327.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ON516_001851"
     CDS             complement(1094..1480)
                     /locus_tag="ON516_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345328.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ON516_001852"
     CDS             complement(1452..2834)
                     /locus_tag="ON516_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3345329.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ON516_001853"
     CDS             complement(2990..4177)
                     /locus_tag="ON516_001853"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3345330.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ON516_001854"
     CDS             complement(4184..5215)
                     /locus_tag="ON516_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3345331.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ON516_001855"
     CDS             complement(5231..6685)
                     /locus_tag="ON516_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3345332.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ON516_001856"
     CDS             6860..7765
                     /locus_tag="ON516_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3345333.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ON516_001857"
     CDS             7856..8533
                     /locus_tag="ON516_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3345334.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ON516_001858"
     CDS             8530..8700
                     /locus_tag="ON516_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345335.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ON516_001859"
     CDS             8814..9710
                     /locus_tag="ON516_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3345336.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ON516_001860"
     CDS             complement(9765..11135)
                     /locus_tag="ON516_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3345337.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ON516_001861"
     CDS             11373..12119
                     /locus_tag="ON516_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3345338.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ON516_001862"
     CDS             12116..12955
                     /locus_tag="ON516_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3345339.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ON516_001863"
     CDS             12956..13906
                     /locus_tag="ON516_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3345340.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ON516_001864"
     CDS             14047..14799
                     /locus_tag="ON516_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345341.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ON516_001865"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ON516_001865"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3345342.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5194 a         3003 c         3226 g         4590 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa ctttttttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagtgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttg
//
LOCUS       DAJSNV010000060        15290 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSNV010000060 DAJSNV010000000
VERSION     DAJSNV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.062"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..252)
                     /locus_tag="ON516_001866"
     CDS             complement(22..252)
                     /locus_tag="ON516_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345343.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="ON516_001867"
     CDS             complement(688..1254)
                     /locus_tag="ON516_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3345344.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="ON516_001868"
     CDS             complement(1254..2210)
                     /locus_tag="ON516_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3345345.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="ON516_001869"
     CDS             complement(2210..2866)
                     /locus_tag="ON516_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3345346.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="ON516_001870"
     CDS             3236..5254
                     /locus_tag="ON516_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3345347.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /locus_tag="ON516_001871"
     CDS             5681..8095
                     /locus_tag="ON516_001871"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3345348.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="ON516_001872"
     CDS             8443..9996
                     /locus_tag="ON516_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3345349.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="ON516_001873"
     CDS             complement(10377..12161)
                     /locus_tag="ON516_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345350.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="ON516_001874"
     CDS             complement(12176..13897)
                     /locus_tag="ON516_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345351.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="ON516_001875"
     CDS             13997..14758
                     /locus_tag="ON516_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3345352.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4974 a         2797 c         3000 g         4519 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc
//
LOCUS       DAJSNV010000061        15216 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSNV010000061 DAJSNV010000000
VERSION     DAJSNV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.063"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ON516_001876"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ON516_001876"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3345353.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ON516_001877"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ON516_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3345354.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ON516_001878"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ON516_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3345355.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ON516_001879"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ON516_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3345356.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ON516_001880"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ON516_001880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3345357.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ON516_001881"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ON516_001881"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3345358.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ON516_001882"
     CDS             complement(8054..8524)
                     /locus_tag="ON516_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3345359.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ON516_001883"
     CDS             complement(8570..8995)
                     /locus_tag="ON516_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3345360.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ON516_001884"
     CDS             complement(9062..9331)
                     /locus_tag="ON516_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3345361.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ON516_001885"
     CDS             complement(9342..10937)
                     /locus_tag="ON516_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3345362.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ON516_001886"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ON516_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3345363.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="ON516_001887"
     CDS             complement(14490..15050)
                     /locus_tag="ON516_001887"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3345364.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3942 a         3437 c         2698 g         5139 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaata aattaa
//
LOCUS       DAJSNV010000062        14729 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSNV010000062 DAJSNV010000000
VERSION     DAJSNV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.065"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            63..1802
                     /gene="yfcC"
                     /locus_tag="ON516_001888"
     CDS             63..1802
                     /gene="yfcC"
                     /locus_tag="ON516_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3345365.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1959..3143
                     /gene="iadA"
                     /locus_tag="ON516_001889"
     CDS             1959..3143
                     /gene="iadA"
                     /locus_tag="ON516_001889"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3345366.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3254..3457
                     /locus_tag="ON516_001890"
     CDS             3254..3457
                     /locus_tag="ON516_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3345367.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3558..4220)
                     /locus_tag="ON516_001891"
     CDS             complement(3558..4220)
                     /locus_tag="ON516_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345368.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4233..5735)
                     /locus_tag="ON516_001892"
     CDS             complement(4233..5735)
                     /locus_tag="ON516_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3345369.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6283..7962
                     /locus_tag="ON516_001893"
     CDS             6283..7962
                     /locus_tag="ON516_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3345370.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8089..8568
                     /locus_tag="ON516_001894"
     CDS             8089..8568
                     /locus_tag="ON516_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3345371.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8787..9467
                     /locus_tag="ON516_001895"
     CDS             8787..9467
                     /locus_tag="ON516_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3345372.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9475..10806
                     /locus_tag="ON516_001896"
     CDS             9475..10806
                     /locus_tag="ON516_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3345373.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10821..11783
                     /locus_tag="ON516_001897"
     CDS             10821..11783
                     /locus_tag="ON516_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3345374.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11894..12232
                     /locus_tag="ON516_001898"
     CDS             11894..12232
                     /locus_tag="ON516_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3345375.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12311..13018
                     /locus_tag="ON516_001899"
     CDS             12311..13018
                     /locus_tag="ON516_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3345376.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13121..14602)
                     /locus_tag="ON516_001900"
     CDS             complement(13121..14602)
                     /locus_tag="ON516_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345377.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4668 a         2540 c         3161 g         4360 t
ORIGIN      
        1 ttcatttgat tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa
       61 atttgaagaa aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac
      121 cccatacgta tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg
      181 ttccagcagg aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa
      241 gtactcgtac tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt
      301 atgtgtttga tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg
      361 ttccggaaaa aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa
      421 aactagatga aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct
      481 atgatacttc taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg
      541 gctttggttt cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg
      601 ctgtagggat cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta
      661 ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt
      721 tagctttacc gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag
      781 aagaggtaat tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact
      841 cgatcgtagc agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga
      901 tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg
      961 cgacattccg attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga
     1021 tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt
     1081 atttccgttc tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata
     1141 aattgatctt attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa
     1201 aaggctatta cattccagaa atcgcttctc aattctttgt gatgggatta gttgctggaa
     1261 tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc
     1321 aatctggtgc agctgattta gctggtacag caatcgtagt aggtatggcc aaagggattc
     1381 ttcttgtctt gggaggatct gatgcatctg ttccttcagc tttgaatacg attttgcatg
     1441 ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg tgcttgggcg atgtacatct
     1501 tccaaagttt gttcaacttg gttgttacat caaattcagg acaagcagct ttgactatgc
     1561 caatcatggc tccactggca gatttagtag gtgtatccag acaagtagca gttcttgctt
     1621 atcagcttgg ttcaggtttt atggacgctt ttacacccgt atcagccagc ttgattggtg
     1681 tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag attccaaatc aagatgcaag
     1741 gattcctgtt tgttttagga actattttca tcatgattgc cattgcaata ggtctgcagt
     1801 agtactggag acctgtaaat aaaaaagtaa tccaaacagt agataaggac tccaagaacc
     1861 gtcattttac ggtaaaatca aaggagtaaa gatcctatcg tttggattat tttttaagat
     1921 cctatcatca gttatactta aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg
     1981 aagtgtttgc accagattac ttaggaaaaa tggatgtact gattgccggt gggaagattt
     2041 tggctgtaga agaacagcta gaaggcggct acgaaggagt ggaagtagaa gaactttcag
     2101 gtgagggaaa agtactaacc ccgggtttca ttgactgtca tttccatatt ttaggtggag
     2161 gaggagaagg cggttatcaa aatcgaacgc cagaagtcac attgagccaa ttgactacag
     2221 ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg agaaggtaga gatatgactg
     2281 cgttgatcag caaagcgaaa ggtctggaag cagaaggaat ctccacttat gtatacgaag
     2341 gttcgtaccg attgccggta aaaacagtaa cagattctat catcaaagac tttttgacaa
     2401 ttgataaaat tatcggaatc ggagaaatcg cagtatccga tcaccgttct tctcagccaa
     2461 gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt aggaggcatg ttatccggaa
     2521 aagctgggat catcaatgtc cacttaggcg gaggaaaaag aaagctagag ctattgacac
     2581 gaatcgtaga agaaacagaa attccgatca cacaattttt gcctactcat gcaaatcgca
     2641 caccagaatt atttgaagct tgcgtggcat ttgccaaacg tggtggcacg attgacttca
     2701 ctgctagcga agatcctgat ttttgggaga agacagatgg tgaagttcgc ttcagcaaag
     2761 cactgaaacg attgatcgag gaagatgttt ctttagataa tttcacgatg acctcagatg
     2821 gacaaggaag cttgccttat tttgatgaaa acaatcattt cctagggtta ggagtaggaa
     2881 gtgccaaggc attgctagta gggatcaaag aagctgtgca aaaagaaagt atccctttgg
     2941 aaatcgcctt acgagctata accagtaatc cagcacgtat attgaaattg gataaaaaag
     3001 gaaaaatcga aattggtgct gatgcagatc tgtgtatctt ggataaagaa acgcttgata
     3061 ttgatacagt tatcgctaaa ggagaaataa tggtccaaga aaaagaagtc aaagtatggg
     3121 ggactttcga aaaaagtttt taatcaacag ctctaactat cttttttaga aaacgagcgt
     3181 tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt agatcaataa acaaactaag
     3241 aggaggaagc acaatggcag atttaaaagg acgttctaaa gatatgaaag ataaagtagt
     3301 cggtgaagga aaagaagcat acggcaaagc aacagacgac aagagcaaag aaacagaagg
     3361 gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt
     3421 caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa ccacttatta ctaattgaat
     3481 caagcaaata aaaaatccga accatatctg cgagacagat atggttcgga ttttttattt
     3541 gatcattatc ctgtttttta aacttcgatt ttttgaaatt ttttattttt tagttcaaag
     3601 acttcatcac agctttttgc tacttcaatc tcatgagtca cgatgataat acagcgattt
     3661 tgttcatggg cgatttcttg gaaaagtttg acgatatctt gtgaagttgt ttcatctaga
     3721 tttccagtag gctcatccgc tacgattagt tgatgttcac agcagatagc acgaacgata
     3781 gcgactcgct gctgctgacc tcccgaaagt ttggtcacct gtttttttgc taatatttca
     3841 tcaattccta cacgagccag tcgatctaaa gcaacttgtc gataattctc ttgcttggtc
     3901 ttagtgattt ccattgctgc tatgacgttg tctagagccg ataaataagg aagcaagttg
     3961 taagcttgga aaataatcga gacatcattt ttacgataac ttcttaaccc gattttgttc
     4021 agtgaacgat cttgatagta tacctttcct tcttttggtg catctagtcc agaaataagc
     4081 gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa tagcgtacat ttttccagct
     4141 tcgaaattca gttctacatc tttataaagc gcatcttgcg gatcttgata ccaaaatccc
     4201 aattgttctg tccgtaacat caaattcctc ctttagttta ataggatttt tcttgggttt
     4261 aatcttaata tccctataga agctaaaaag acagaaaaca gactgattcc aaatgctact
     4321 ccggctaata gtcccaattg tgcaggtttg acattgattt ccaattcgct gacttgatca
     4381 gaaacagcaa atggattatt cgtggttcct tgttgaccat cgcgttgacc gccttgttct
     4441 tgtctgttac ctggcatttg tccaccgcca ggagcttgct ggttttgtgt cgtggtttct
     4501 gtctgttggt caagtagctg ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa
     4561 gcaactccta atgcaagaat catacaaata gccacttcgg taaacaattg gagaatgatt
     4621 ttacttctgg attctcctaa agatagcaag acgcctaact catgccgacg ctcccgaatg
     4681 gtaatcataa tgatcagagt aagtatgata ataccggcta cagccactag taaaacaaca
     4741 tttttagaaa aacttgccac gttgtttaaa ggttccaaca ttgactgata catggaatca
     4801 tttgattgta agctaaatgt atctgtgtcg atcagcttct cagcttcttt gagaaactca
     4861 tccattttat ctggatcaga tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat
     4921 gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg
     4981 aagttgaatt tcatacccat actgccgctt gtttcgctgg attcataaat tcctttgatc
     5041 gtcatttcat aagtggtatc ttcatcttca gtactcgtga ttgtgaaagt atctccgact
     5101 gccaggtcat ttgtttctgc tagagtagaa tcaatgacta cattgtttgt gcctttgtcg
     5161 ctgtcatcta ttccttcacc atccgtgatt tttgctgttc catcagaaaa attactattt
     5221 tgtgcagttg ctgacacacc actgatttga aagtctgctt gagtcatttg cggattcccg
     5281 cccattcctc cagcaggttc accgcctgca tcagtagttt ctgaactgtc ttcacttgaa
     5341 tcagaactag tgatggcagt tattccatct tttgccatag ctgaggcgga actttcaaag
     5401 gaataactct ttacattatc taattcagct attttttccg cgtccgttaa ggaaactggt
     5461 gtaagagaaa aactgcctgg atctggacgt tcttcagtgg tagaaccgct tgaagaagtt
     5521 tgttcctgtt ttttaaatga agcttctcga ttggcactta aagtcactgt tgctccaact
     5581 tctttttgtg cttgattcgc tgcttcttct gcagcgcttc gaatcgtgag gccagctagc
     5641 acaaaaataa gaatggctgt gaacacggca aacaataaca atgaacgtcc ccattttact
     5701 tttgtacttt tccatgcacg tttcataaaa ttcatatttt gcatccctcc tagtagatgc
     5761 atttgataat agacaaccta tcttaaagct agcttaaatc ttatgctgtt tgcacaccat
     5821 caacatgagg ttctgtatat ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa
     5881 tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc
     5941 ctagatgcta cagagggatt tttacgtgca tagttgttct aacaattgct gatactagcg
     6001 atttagttta tttgttgtat aataatgggg aacagctgaa tacttatata agtattctgg
     6061 ctatagcgtt tcgcaaaaga tccattttgt taatgaagta tatccaaaat aaaatgccca
     6121 tccaactgtc agaacgcgga tgtgctggta attttcctgc tgattctcca taagttgaga
     6181 aatagtgatt ttgcatatgc ttcattggaa tggctcatat atttagataa agaagaaaat
     6241 cgacatcttt aaacttggat gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt
     6301 atcccattag gcggcgttcg tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag
     6361 gatattttta ttttagattg tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac
     6421 gtcgtaatcc ctgattttac gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt
     6481 ttaacacatg gacatgcaga tgcgatcggg gcacttcctt acttgttgtc aaaaatttct
     6541 gtaccagtat tcgggacaga gttgacgatc gagttagcaa aattgaatgt cagtcgtaat
     6601 gaagcagcca aaggatttaa ggatttccat gtggtagatg agcatacaga aatagacttt
     6661 ggtgaaacag tagtgagctt tttccgtacc actcatacga taccagattc agtggggatc
     6721 aatctgaaaa caaaagaagg tagtatcgtt tatacgggag attttaaatt tgaccaaacg
     6781 gctatcccaa tgtatcaaac agattatgca cgattggcgg aaatcggcaa agaaggagtc
     6841 ttagccttac taagtgaatc ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc
     6901 caaatagcag atgaagtatt tgatacgatc aaatattggg aagggcgtat catcgtcgct
     6961 tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg atgcagcaga aaaagcacaa
     7021 cgaaaagttg tcttgactgg tcaagatttc gaacgaatta ttcgcacagc gatgcgttta
     7081 gaaaaacttc aattaccaag tgaagatctg cttgttacgt tgaaagacat gaaaaactat
     7141 gatccagaag aactgatcat tttagaaact ggccgaatgg gtgaacccat caagtctctg
     7201 caaaaaatgg caaacggtac acaccgtaat gtcaagattg aagaaggaga tttggtttat
     7261 atcactacta cgccaagcat cgcgatggaa accatcgtgg cgaaaacgga agatatcatt
     7321 tatcgagctg gtggaacagt gaagttgatc tctgagaata tgcgcgtttc tggccatgcc
     7381 aatcctaatg acttgcaatt aatgatcaat ttgatgaaac caacatattt tgtgccagtt
     7441 caaggagagt atcgtgaatt agctgctcat gcagatttgg ctcatgcagt agggatgcca
     7501 tacaaaaata tctatattac tggacgcgga gacgtgctgg aatataagaa caagcgtatg
     7561 agtgttgcag gaactacaag tgcagaaaat gtaatgattg atggattagg cataggtgat
     7621 atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg
     7681 gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa aacctcaaat tacttcacgg
     7741 ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa
     7801 acaatcgttg agaaacattt agaaagcaat gattttgagt ggagcaaatt aaagcaagac
     7861 atccgagatc aattaggccg ttacttattt gaacaaacaa aacgccgccc agtgatcttg
     7921 ccagtcatta tggaagctac tcaacgtcga aataaaaaat aaacaaaaag agtaagatga
     7981 acctctctat agagtgctcg tcttgctctt tttttgcgaa aaacaaataa tttttgaata
     8041 cagcatccct cgctttataa taaacgatag aataaaggga gtgaatcaat gatgagtcaa
     8101 acaaaagtcg aacgttattt agaagaacaa aagatcagct atcaaccact tgattttagt
     8161 catgtggtag aagaatcttt cacagaagaa ctacaaaaaa gagggattga tccagctttg
     8221 atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg tgatcatcgc attgattcct
     8281 ttgaacgatc acttagatta caaaaaaacg agacaagtga cgaaagatcg taaagttggt
     8341 tttcctggaa tggattttgt attaatgcat acaggatatc cccacggagc aaacacccct
     8401 attggaataa aactcgcaca tcctgattat tgttttttag cagacagttc tttaaaagaa
     8461 aaagaagaag taattgtgtc aagcggtgaa atcgggagaa gccttcggat aaaagtagcc
     8521 gatttagaaa agatcgtcca tcctatttac gctgatttaa ttagttaagg gacgagaaga
     8581 gaagctaagc agagcagaga cctcaaactt gagattatat ctgcttggta cttggtgcat
     8641 cctgtattat ctgttacaat tgctttgtac atactgtact atcaatccaa tgaaaatata
     8701 tttagcagct atattttttc attgatgtgc aagtgatgaa atcgttttta ataaaaaaga
     8761 tacagagaaa acgcgaggtg taaaatatga aaattacgta tcatggacat tcctgtttaa
     8821 caattgaaat ggaaaatggc caacgcgtga tgattgaccc gtttattaca ggaaatccat
     8881 tgatagactt aactgcagaa gaagtaaaga cagattggtt gctgattact catggtcata
     8941 gtgaccacat aggagatatg attcctattg caaaagaaaa tcaagccaca attatcagta
     9001 ttgttgagat tgctgattat gcgaacagca gaggagtaga ttcattcggt atgaatatcg
     9061 gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt acatgcacag catagttcaa
     9121 gctatgaagt agacggtatc gttcagtata tgggcgagcc tagcggaatt attattcaag
     9181 cagaaggaaa aacgatttat catgcagggg atactgctta tttcagtgat ctaggattgc
     9241 ttgcagaaga attcgatatt gatgtcgctt tcttgccgat tggcgataac tatacgatgg
     9301 ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc caagaaagta gttccaatcc
     9361 attacaatac tttccctgtc attgaacaag atcccgaatc ttttgtagag ttactggaag
     9421 atagtgaagg aaaaataatg agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg
     9481 acaaaacatg atttgatttt agaatatatt gaaagtttac ctgttggcaa tcgaatttct
     9541 gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa
     9601 gatgcagaaa atgtcggact agtttcaaca attcaacgag tagggaccat ccgtatcgaa
     9661 cgcaaattaa aaaaacatat tgaacgtttg acatttggcg aggtcgtcag gatcattgaa
     9721 ggggacgttt taggcggggc ggcaggatta gacaaggtct tgaataaatt cgtcatcggc
     9781 gcgatgacta aacatgccat ggaacgctat attacgccag gatcactaat gatcgtcggc
     9841 aaccgggcgg aagtccaaaa attagcattg gaagatggcg ccgcagtatt gatcacagga
     9901 ggattcaata cgactgctga gattgcaaag ctagctgatg atcttgaaat gccgatctta
     9961 cgcacctctt atgatacatt tacagtcgca accatgatca accgagcgtt aagcgaccaa
    10021 ttgatcaaaa aagatatcat gcttgtcagt gatatctata tgccagctga aaagactcat
    10081 tatttgcagc agacagatac gatttctgat tatcagcgtt tgtcagaaga aacgcagcat
    10141 tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg ggattgttac ggcaaaagat
    10201 gttttaggga aatcgccaaa ccagatcatc gatcgggtga tgacgaaaga accaatcagt
    10261 gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa tgatctggga tgggctagaa
    10321 gtcatgccgg tcgtttccga tgacttgaca ttggaaggct tgattacccg gcaagatgta
    10381 atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg cagatacgat ttcggatcag
    10441 atatctggaa atatccatac gattgatact gaccgtgaag gaaatcgttt ggaaaaccct
    10501 aagttcaaat tcgtagtagc accacaaatg gtcaatggcg ttggaaccat ttcttttggc
    10561 gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga
    10621 aatatcttga ttgaacaagt gaacctacat tatttgcggc tgatccaatt ggaaagcgaa
    10681 ttagacattc gtccacgtgt attggaaatc ggcagacgtt ccgctaaact ggatatcgaa
    10741 gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt
    10801 tcttagaaag caggagtgaa atgacagtac agcaagaaat agtagataca ataaaaaaat
    10861 acgataaaat cattatccac aggcatttgc gccctgatcc tgatgcatta ggatcacaag
    10921 taggattagc cgaactatta cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg
    10981 aaacgattga agggcttcgc tttttagcag aaatgcaaga agttgaagat gtcttctacc
    11041 aaggagcgct agtcatcgtg actgatacag ctaattcacc cagaatcagt gatcaaagat
    11101 atcagttggc agaaaaactg atcaaaatcg accatcatcc caatgatgac ccttatggag
    11161 atcttgtgtg ggtaaataca aaagcaagta gctgtagtga gatgattgca gaatttgctt
    11221 tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc acggttactt tatgcaggaa
    11281 tcgtcggaga tacagggcgc tttttgtatc cctccacgac gtcccgtaca ttagagattg
    11341 cttcgatatt gagaaattat ccatttgatg caagtgcttt gaacagagag attgaacaaa
    11401 tgccgatgaa agtcgctaaa ctttccggtt atctttatca aaatatccag gtggatgaaa
    11461 atggtgcagg aagaataatc cttccgcaag aaattcttga taaatatgga atcgatgatt
    11521 cagagacagc ttcgatcgtt tcacttcctg gagtgattga cgaagtgctg gcttggggga
    11581 tttttgttca gcagccttca gggtattacc gcgtacgttt acgttctaag gggccgatca
    11641 tcaatgaatt ggctaaacgc catcatggag gaggccatcc attagcaagt ggtgcgaatg
    11701 caaaagattt agcagaagta gaggaaatct ataaagagat ccaagatata tgccaatcgt
    11761 ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc
    11821 acaaatgtat cttttcaaga tcttcttttt ccgttacaat agtgctcact aaagatttta
    11881 aaggagaaag aagatgacat taccaaattg cccagaatgt ggctcagagt acacatatga
    11941 agatcgaggc ttgttgattt gcccagaatg cgggaacgaa tggaacaaag aagaacaaga
    12001 aactgtcata gaagacggat tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg
    12061 cgatagcgtg acagtgatta aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca
    12121 aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat cataatatcg attgcaaggt
    12181 agaaggattt ggaccgatga aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt
    12241 caaggtcttt tcacattctt ttcctactac taatgataaa ataaagtaaa cgaaaaggag
    12301 tgaaacgaaa atgtatggtt atggaatgta tggtttttta gatccgacat ttcttttggt
    12361 cattcttgga ttagttattt ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa
    12421 atacgatcaa gtgagaagca aaaacaatgt gactggtaca caagcagcac aatatatatt
    12481 acaaagtcaa cagataaata atgtcggtgt tcaacaaatt gccggagatc tcacagataa
    12541 ctacaattca ggtaataaga tgttgagttt gtctcaagca actgcacagt ctacttcagt
    12601 tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg gtacaagatg cttcgggtta
    12661 tgtcccattg aagattcgtg cagcacttgt tccagcagct aactttggtt cgatgatttc
    12721 gttccccttg attatggtag gtgtattgct tagctggaac tctacgttaa tcaatatcgg
    12781 gattttcgct ttttctttag cattactttt ccaattggtg acattaccag tcgaatttaa
    12841 cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga ctactgacgg aagaagaagt
    12901 accgatggca agacacgtat tatttgcagc agcactgact tatgttgcag cagcgctttc
    12961 gacattcctg cagctgttgc gcttgatctt attgttcggc ggcaaccgaa gagattaaaa
    13021 taaagaagtt gtgaaatcag cgctttgatc tgagctaata ccgagagtta gctgattgaa
    13081 taccatttat ttggaataag aggatgtgac aagaattttg tcacatcctc tttgttttgt
    13141 tctcataacc ttttttcata tacagttcat gaaccgcttt tattaacttg ccaaataaca
    13201 ctgtattgtg cgttgtatcc agatagaaag tctccgtttc atctgaaatt ttctcatgct
    13261 gatctgtaat gatcaaatca gcatgttctg aatatgcggt aattttaaca gcatctttgc
    13321 gaaataactg ctgcaaatgg ctttgaagat gataatttcc tgtaaactgt ttggttatct
    13381 gcaaatggat gagtaactgc tctttcgttt ctgtttgtag taagtgttgg atcaattgac
    13441 taaggtattg actgatttgg tttctttccc cattttttga aaaaattgta tcgatttttt
    13501 ctcttaattt cttttcctgt ataaaagaac tagaatgatt ggacgtggct tctggaaagc
    13561 aaagtgtaga aaaactactg ctggattcac ccaacagttc gaaaaaaaca actgccaaca
    13621 caagaaaata aaaataaatt ggtattttat tttctaaatg gttgagagga aactgttgag
    13681 ttaagtaagt catcaattga ccgacttgtc gacaaataag gtttttgctt gtggaaaata
    13741 aaaaaccaat ctcctgtttt tgtcgaaaag agatttgatt tggaaaaaaa caatagaaaa
    13801 aataattaaa gtaggctctc tctgtttctg tctcttcaca agggcgactc actgaaaaag
    13861 aagtaaagta ttctgaataa tcatgtgtat gctgcaataa gtccatcaat tcagtgattt
    13921 cagaagaagt ttttggaaaa tatgcctgtt gtttgtttcg taatatgaat aaagttaaca
    13981 gcaagtagat atttttttct tgttttgaaa ttttttctat ttgctcatct gtactgttca
    14041 tttttagtgc atttaattca agtgaggaaa atggccaatc gttacctcca aatgtatctg
    14101 aaagaaaaaa gtaaagatag agacggatag ataactcatt tccttcaaga gtacagcaat
    14161 tttttacaga taggatccgt aaatggaatt tttttaagga atgattgagc tgttttgtca
    14221 gtttttcgag ataggagggc gatatataaa gttgttcaag taaatatttt tttggtacag
    14281 cagtttgtgg ataattgatt agtaacatca aatagttaaa acgattggat tcttttaggt
    14341 agcataattt taacttagaa aaaacagcta actttttagt ttgatcggtt tctttggttt
    14401 ggatctctcc tttatgatta gaataaataa attcatcaat caataaaaat tgcttgagtg
    14461 atacatttat ttttcggatc agttctttga ttgtccgttt tggaatatgt aagtcattgg
    14521 ataattcttg taaagaacaa ttattttttt gaatgaccat ttgtagaaca agtaactctt
    14581 tttttcttgc ttcaattagc ataaatacct cctttttatt ctgtttttgt atatagttag
    14641 acaaaaactg tgagtggaaa gtcatttcag tctgtgttat tctcacagag tgtcgccttc
    14701 ttttttatat aacgttattt aattttttt
//
LOCUS       DAJSNV010000063        14622 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSNV010000063 DAJSNV010000000
VERSION     DAJSNV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.066"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..978
                     /locus_tag="ON516_001901"
     CDS             82..978
                     /locus_tag="ON516_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3345378.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1128..2069)
                     /locus_tag="ON516_001902"
     CDS             complement(1128..2069)
                     /locus_tag="ON516_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3345379.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2195..3316
                     /locus_tag="ON516_001903"
     CDS             2195..3316
                     /locus_tag="ON516_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3345380.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3574..5076
                     /gene="eat(A)"
                     /locus_tag="ON516_001904"
     CDS             3574..5076
                     /gene="eat(A)"
                     /locus_tag="ON516_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3345381.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5104..5670
                     /locus_tag="ON516_001905"
     CDS             5104..5670
                     /locus_tag="ON516_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3345382.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5881..6942
                     /locus_tag="ON516_001906"
     CDS             5881..6942
                     /locus_tag="ON516_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3345383.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6945..7841
                     /gene="murQ"
                     /locus_tag="ON516_001907"
     CDS             6945..7841
                     /gene="murQ"
                     /locus_tag="ON516_001907"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3345384.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7855..9837
                     /locus_tag="ON516_001908"
     CDS             7855..9837
                     /locus_tag="ON516_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3345385.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9902..10348)
                     /gene="nrdI"
                     /locus_tag="ON516_001909"
     CDS             complement(9902..10348)
                     /gene="nrdI"
                     /locus_tag="ON516_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3345386.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10559..11983
                     /locus_tag="ON516_001910"
     CDS             10559..11983
                     /locus_tag="ON516_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3345387.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12164..12937)
                     /locus_tag="ON516_001911"
     CDS             complement(12164..12937)
                     /locus_tag="ON516_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3345388.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12934..13839)
                     /locus_tag="ON516_001912"
     CDS             complement(12934..13839)
                     /locus_tag="ON516_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3345389.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13993..14313)
                     /locus_tag="ON516_001913"
     CDS             complement(13993..14313)
                     /locus_tag="ON516_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3345390.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4668 a         2608 c         3099 g         4247 t
ORIGIN      
        1 tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac
       61 agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa
      121 tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa agatttacta
      181 cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct gatcgttttt
      241 ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac caatgcaaaa
      301 attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca
      361 caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt
      421 gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca
      481 aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat
      541 aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg
      601 aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc
      661 gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc
      721 atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat
      781 cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg
      841 catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg
      901 gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc
      961 tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag
     1021 cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa
     1081 aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca
     1141 aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata
     1201 tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga
     1261 tggtttttga tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca
     1321 taagtagaag caatatgttg gtacacttct tcttgatcta gtatgattcc ttcttttgct
     1381 gcgatagaag caaattcaga tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg
     1441 cctagttcag caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca
     1501 caagcttttc gccaaatgga ataacgaacg ttatggctat agatcggatt taaatttgct
     1561 tcttgaaaga cttctacaac tttttttgta aatgcttcgc cttctggttc caagttctct
     1621 agttcgattt ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc
     1681 cacatcgtga tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca
     1741 tgacctaatc catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc
     1801 gaacaaaaca tatcatctaa ttgattggct tttgttaatg ctacgatcaa gtccacgtgt
     1861 tcgttgcttt cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta
     1921 ccgttaaagt cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga
     1981 tcgatcaaga ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt
     2041 gctccggccc cagctattgc gattttcatg ttttttctct cctttattca atgagttccc
     2101 ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa
     2161 agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc
     2221 atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga
     2281 tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc
     2341 aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg
     2401 tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga
     2461 atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact
     2521 tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca
     2581 aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc
     2641 cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact
     2701 tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt
     2761 taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg
     2821 aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa
     2881 agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg
     2941 tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat
     3001 cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat
     3061 cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata
     3121 tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga
     3181 ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga
     3241 acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac
     3301 catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag
     3361 gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga
     3421 ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc
     3481 gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa
     3541 taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg
     3601 acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac
     3661 acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat
     3721 attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat
     3781 ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg
     3841 gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc
     3901 ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt
     3961 ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc
     4021 tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc
     4081 agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt
     4141 caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat
     4201 gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca
     4261 gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc
     4321 ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca
     4381 cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa
     4441 gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct
     4501 caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc
     4561 gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt
     4621 gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga
     4681 gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat
     4741 cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac
     4801 aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt
     4861 atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat
     4921 acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg
     4981 atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa
     5041 gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa
     5101 ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga
     5161 cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg
     5221 actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt
     5281 ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt
     5341 gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg
     5401 gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct
     5461 gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg
     5521 aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata
     5581 agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta
     5641 gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat
     5701 ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa
     5761 tataaactca acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt
     5821 ttctttagat atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat
     5881 atgtttggct tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc
     5941 cgccaaatgg catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat
     6001 gatgtttcgg catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa
     6061 ctagaattga tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa
     6121 gatctaacgc ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc
     6181 aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc
     6241 acgcctaaag atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct
     6301 tggcataatt attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa
     6361 aatcaatggc tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag
     6421 ttgagaggcc cactatatca aggattgcca actttggaag aacatcgagg gatccatcct
     6481 cttgcaggcg ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca
     6541 ggtctgtcag aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg
     6601 ctccatacgg aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat
     6661 cggcaagacg atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg
     6721 cctatcccag caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa
     6781 aagtatttgc gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa
     6841 aaagtgaatg tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa
     6901 gctggtatga actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt
     6961 tagagaacct aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta
     7021 tttctcaagc cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa
     7081 aagaactgac aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg
     7141 gacgtctgat ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg
     7201 aatgcgtacc gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag
     7261 aagaagcaat gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag
     7321 atctaatcaa gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc
     7381 gtacaccata tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa
     7441 cgatttcttg caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg
     7501 atgcaggtcc agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac
     7561 ttatcttgaa tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact
     7621 tgatggtcga tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca
     7681 tgcaagcgac agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa
     7741 atgttaagct ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa
     7801 aattagctga agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca
     7861 aaaaacgaac tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg
     7921 ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat
     7981 gatcttgtaa atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat
     8041 gaacagcttc aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt
     8101 gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat
     8161 tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa
     8221 aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt
     8281 gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta
     8341 gcaaacttag tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg
     8401 atgaacatct tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct
     8461 gcacaagttt ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg
     8521 acagggatga atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct
     8581 ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa
     8641 aaactgcata aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg
     8701 attgccatcg gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat
     8761 ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt
     8821 ctaggcacat tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc
     8881 catgtgcaaa tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct
     8941 ggtgcaggac aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa
     9001 ttaacagaaa tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg
     9061 atttacggag taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt
     9121 atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca
     9181 ggagccgctt tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta
     9241 ttggcagctt atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt
     9301 ttagaaaaag aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta
     9361 gtacctcaaa cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt
     9421 gtttctttag ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac
     9481 tttgtagaac cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc
     9541 ttcccaacga aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat
     9601 atgggaatca acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg
     9661 cagcaagtga cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca
     9721 ggcaaagaaa catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc
     9781 ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat
     9841 gatagaaaca atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt
     9901 attatttatt gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc
     9961 gtaattcata atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta
    10021 gacaatactg attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat
    10081 gattgtcttc atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt
    10141 tatctaaacc atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt
    10201 ctttcgtaaa atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat
    10261 cttctgcatg cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc
    10321 ctgaaatcga tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtata
    10381 gcatcttttt taagtttttt ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta
    10441 taatgagaaa aaagccggta gaattatatt tctattaaag tttttattaa ttaagtaaaa
    10501 ataaaaaagg tttgttatac tttttgataa aagcggtttt attttggagg atgcttacat
    10561 ggatacagag caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg
    10621 ttcagcgatt ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg
    10681 cccagctatt ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat
    10741 caataatatc aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga
    10801 aggttttggc cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt
    10861 aggcaacgta gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt
    10921 tgaaggtggg caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc
    10981 atggctagtg aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg
    11041 taaattagtg ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca
    11101 attcattgat aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt
    11161 ttccagtcaa gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg
    11221 agcctctgtc gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct
    11281 agggatattc ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat
    11341 gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat
    11401 gatggttggt aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg
    11461 tgtttggctc tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg
    11521 acttttgcct aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt
    11581 attgacgacg ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta
    11641 taactttgct tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt
    11701 gtaccaaatg aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt
    11761 agcaagtgcc tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt
    11821 tcttgttctt ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga
    11881 gcctgtacaa catatccact tagataagat tttacttata ggtgtgacga tggtcggagt
    11941 gattgcaatg gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat
    12001 aaagagaggt tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac
    12061 aaatgttgtc acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa
    12121 taaggctgaa cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct
    12181 taaatacgag atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca
    12241 tttgattagt agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt
    12301 gcagattcaa catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa
    12361 taaggatacc agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg
    12421 cgccggaact attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac
    12481 cgatataact acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa
    12541 ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa
    12601 tagctaaaag gtataaataa acagaataaa taactaatgt gatccatttg cttactaata
    12661 tttctcctcg agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca
    12721 tcgaaatgat cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa
    12781 aaagctgagc agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg
    12841 ctgttcctat gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga
    12901 tcaatttaaa taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga
    12961 atccagaatt tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac
    13021 atggatattt tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt
    13081 taaaagcttg attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg
    13141 agcagctaga tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc
    13201 agccattgta cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat
    13261 tttttcaagc tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa
    13321 gtcggtaatg atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc
    13381 atctaaaata acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt
    13441 cattcccaac gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt
    13501 cacgatagtg tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata
    13561 gcctgttaag aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc
    13621 tttatgacta gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg
    13681 gatcagtcct aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa
    13741 tccaacgatt ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc
    13801 aaagttcttg gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat
    13861 agatagtaca agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct
    13921 tattttttca taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact
    13981 tattccaagt gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat
    14041 ccccacaaag aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat
    14101 gaccgaacca actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact
    14161 tttgattgcc tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga
    14221 ataattgatt ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa
    14281 aattcctgct atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa
    14341 aatacacaaa aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta
    14401 tcctacccgg tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc
    14461 ggcaatctca tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa
    14521 aagaagatga atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa
    14581 aaaacagtaa ctagacctta tttctatata ttatcgggca at
//
LOCUS       DAJSNV010000064        14353 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSNV010000064 DAJSNV010000000
VERSION     DAJSNV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.067"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(324..893)
                     /locus_tag="ON516_001914"
     CDS             complement(324..893)
                     /locus_tag="ON516_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3345391.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ON516_001915"
     CDS             complement(967..2256)
                     /locus_tag="ON516_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345392.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="ON516_001916"
     CDS             2610..3458
                     /locus_tag="ON516_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3345393.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3603..4544
                     /locus_tag="ON516_001917"
     CDS             3603..4544
                     /locus_tag="ON516_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3345394.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            4534..6705
                     /locus_tag="ON516_001918"
     CDS             4534..6705
                     /locus_tag="ON516_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCR3345395.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6817..8973)
                     /gene="copB"
                     /locus_tag="ON516_001919"
     CDS             complement(6817..8973)
                     /gene="copB"
                     /locus_tag="ON516_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3345396.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9116..11302)
                     /locus_tag="ON516_001920"
     CDS             complement(9116..11302)
                     /locus_tag="ON516_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3345397.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11302..11511)
                     /gene="copZ"
                     /locus_tag="ON516_001921"
     CDS             complement(11302..11511)
                     /gene="copZ"
                     /locus_tag="ON516_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3345398.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11524..11964)
                     /locus_tag="ON516_001922"
     CDS             complement(11524..11964)
                     /locus_tag="ON516_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3345399.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12038..12511)
                     /locus_tag="ON516_001923"
     CDS             complement(12038..12511)
                     /locus_tag="ON516_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HCR3345400.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12821..14287)
                     /locus_tag="ON516_001924"
     CDS             complement(12821..14287)
                     /locus_tag="ON516_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3345401.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4089 a         3009 c         2543 g         4712 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt tggcagccaa
     3841 aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat agtctaggac
     3901 ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg cctgcttttt
     3961 gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt
     4021 accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat tctttcaacc
     4081 gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa tacgattatc
     4141 aagaagaaga atgttctcct cttttttgct tttggggttc gcacagtcca aaatttgaac
     4201 ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag ccggaacttc
     4261 tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat tttgcttctt
     4321 tggaagctgg cgatgagaaa gcttttttcg cacacctaaa aaaatatgca aaaaaacaaa
     4381 tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa acattgtcaa
     4441 aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc caagaaaaag
     4501 acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaatggga aaaatggctg
     4561 tgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt agtagctaat
     4621 caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct tattgtctgc
     4681 tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat aggttgtttg
     4741 tttttgtatt ttccttcgaa gacattttcc ttattatgga tcttacagtt tttttcgtca
     4801 gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc agaaaagaca
     4861 gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt ggattacgac
     4921 tatttgtacg caccctttct ttctttgatg gcttacttca tgatgcttac cgtttttcgg
     4981 gaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt gtatctggca
     5041 gcaaggcaat ttttctccat gcggttgttg tcgggaaaca ctcgattcca gtcattgact
     5101 acagttctgc ttatttgttt cttaactatt agcgctatct tgcctttata tcttccgaaa
     5161 gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg
     5221 ctttatgata cactccatga gtatcaatta accggaagtg caagaacagg cttcagtgag
     5281 aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt tacagcgaac
     5341 caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg ttctggatgg
     5401 atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga catcacgagc
     5461 taccaaaccg ttatggaaga caaagaaaac attacattga ccctaaaaaa acaagctttc
     5521 ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag tggacaaacg
     5581 ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc gacacgttct
     5641 gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag cagtcagtta
     5701 aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa
     5761 cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat tcgtaaagtc
     5821 gaagctgtcg aacagtattt aaaaacaagc ggaaatttcc gctattccaa aacggatacc
     5881 ggacatactc cgaaagaaca cgattatgtg gattatttcc tctttgattc aaaagtcggc
     5941 tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg tatcccagct
     6001 cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca agggaatgag
     6061 gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt tcctggattt
     6121 ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc acaaccagtt
     6181 gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag cgaaaatatg
     6241 gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac aacactcaca
     6301 ccagcttata aaatcgtctt attcagcctt ggggtgtgtt tatacgtctt gttgttcttt
     6361 ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct tcattttttt
     6421 gcttctacta atacgattgc atatcaaaaa cttcttaacc atttggaaac attggtaccg
     6481 cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga attcaagcta
     6541 gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg acgacaagca
     6601 gatccggtaa atttcgataa aaaaacctat ttagaaatta cgcatataat aagaaaaacg
     6661 gtatacaaaa aagttctgac aaaagggagc cgcttcaaaa aataagataa aatttctgga
     6721 gaatctactt ctggaatgcc gagaaccagc tgcttgagta tcgattattc gaaaaaaagg
     6781 ttgtgacacg ttttttgtca caaccttttt tcattattat ttgattttca atgtcaaagc
     6841 atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa
     6901 tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc
     6961 tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa
     7021 atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat
     7081 atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt
     7141 gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc
     7201 ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac
     7261 tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt
     7321 caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc
     7381 agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt
     7441 caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt
     7501 cacttgttgg tttttgactg ttgcctctag cccaactcct gaaagatttt ttagattttc
     7561 ggcttgataa ggcttgattt catgttcttt caaataattc ataataccaa cagccaatgg
     7621 atgattggca ttcgcttcca atgctcctaa atattgaagg atttcatttt tattgaacgc
     7681 atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt
     7741 gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag
     7801 taatccgttt ttagctgcta ttgatgtaga gcgagcaaca actaatggga tagctaaacc
     7861 tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag
     7921 atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa
     7981 cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt
     8041 tcgcaccatt tccataacct tcgctagata accattttca ccagttccag ttacttcgat
     8101 ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt
     8161 gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat
     8221 tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac
     8281 ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttta ctgattctgg
     8341 cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat tcatttcaat
     8401 ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac
     8461 gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac
     8521 tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt
     8581 agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac
     8641 gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga
     8701 aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt tgaaatttcc
     8761 catgtgcatg gaatgatcca tgccgccatg atcgtgatcc atgtgcatcg aatggtccat
     8821 ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat ggtccatctg
     8881 gctatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac
     8941 tacttctttt ttatgttcat gtcccatgtg catggaatgg tccatctggc tatggtcatg
     9001 tttcatctcc atctgttttt catgagattc tgtactttct ttgtttacta cttctttttt
     9061 atgttcatgt tttttctcca tatcgttccc attcattttt actccatccc ttctcttact
     9121 ttttcatgtg ccgatttaag ctcagggagt tcaacaatac actgacggaa ctaaacgcca
     9181 tcgctcctcc tgcaatgatt ggattcaaaa agcctagtgc ggcaaaagga ataccaatcg
     9241 tattatagac aaaagcccag aaaagatttt gtttgatttt tctcaatgtc accctagaca
     9301 actcgattgt ttgtgcaatg gatgccaaac tgctactcat cagcgtcaca tcggctgttt
     9361 ccatcgcgat atccgttcca cttcccattg ccataccaac atcagctaat gctaaagccg
     9421 gtgcatcatt gattccatcg cctgccatac cgactttttt gccatctttc tgaagttttt
     9481 ctacgtaggc tgctttatcc tctggaagta cttcagcaaa gatatgcttt ggatcaatac
     9541 caacttgttt accaatcgtt tcggctgcca gtttgttgtc tcctgtcagc ataaaaacat
     9601 ctaatccttt attctggagc tgcttgattg cttgtttggc ttctagtttg atttgatcag
     9661 ccacggcaat caacccgatc acttgctttt catcagctaa aaacatgact gtttttccct
     9721 gctgttctaa ttctaacgcg tgttcttggt attcatcaaa tgataggttc aattcaatta
     9781 atcgtttacg tgtacccgca aaatagcgta ctccgttaat cgtaccgctg attccagcac
     9841 ctgggtgagc tgtaaaatca atcatatcgt atgttgctgc ttcttgcagt tttccgtatt
     9901 ccacgattgc tttacctagt ggatgttcag aagcttgttc taaagtataa aaaatagata
     9961 ggacttcttt agaaccgact agatctgtaa ctttgggctt tccttctgta atcgtcccag
    10021 ttttatccaa aacaatgctg tctaagtgag cagctgcttc taatgcttct ccgcctttaa
    10081 ttaaaattcc atttcgtgca ccaacacccg ttcctaccat aatagctgtt ggagtcgcta
    10141 aacctaatgc acatggacac gcaatgacta atacagagac actatggatc aatgcgagtt
    10201 gccaatcacc agtgatcaaa cctgtagcta tgagcgtgat aaacgcgatg actaatacga
    10261 tcggaacaaa aatccctgag atccgatctg cgattttttg gattggggct ttgctccctt
    10321 gagcatcttc caccatttga atgatttgcg ctaatactgt ctgcttgccg acttgtgtga
    10381 cttccatgtg tagcagacca ttcgtattga ttgtcccgcc aaaaagcgtg tcatccggat
    10441 tcttttctac aggaagattt tcaccagtaa gcatactttc atctatcgca ctgcttcctg
    10501 aaataattcg accatctgct ggaatctgct caccaggacg gatgactagt tgatcaccaa
    10561 cgactacttc ttcaatcgct aacgtttctt ctttcccgtt tcttatcact tgggcggttt
    10621 ttgtttgaag agacatcaat tgtttgattg cattacctgt tttcgttttt gctgtatgtt
    10681 ccaaatattt gccgagcaaa atcagcgtga tgatcatact gctgctttca aaataaagat
    10741 cttgggggtg tccacggaaa aatccgttgt aaatgctcaa tgcaaaggca gccgatgttc
    10801 caattgctac caatacatcc atatttggtg ctttcgactt gattgcatgg tacgcacctt
    10861 tataaaaacg agcaccgaca taaaattgga caggagcaga cagcactaac tggacgattg
    10921 gaaaatgaaa aaagtgaaca atcgccgcat ggcttcctaa catcatagca atcattgaca
    10981 acatcaacgg caaagtcaaa accgtactca atataagatc gaatagcatt tttttcaaat
    11041 acgcttgctt ttcttctgca attttttgtt tgtgggcttc atcgtacaaa attgcaccat
    11101 agccgatgtt ttctacgctt tggattaatt tttcagtggt cgtcccttca tatttgacac
    11161 ttgctttttc agtggccaag ttgactgtgg cattgaccac accaggttgt ccgttcagct
    11221 ctttttcgat cctagcagaa caatttgcac aagtcatacc tgtaatgacg aacgtatcca
    11281 ttttggtttc tgctgttgcc atcaaatcac ctctgcttta taacccaatc cattgattgc
    11341 ttgacaaatc ttttcggatt gaacttttgt ttcatcaaac ttgaccgtac cgtttgcttt
    11401 tttcaaattt actttcactt tttgcacgcc gtcaagtgcg cttactgttt cttctactct
    11461 tgctgcacaa tgattgcagc tcataccttc gattgaaaat tgttgtttca ttttttcttc
    11521 ctcctattga tgttgtttac attcacattg acctgggata cagttacatt cgatcgtatc
    11581 tactggttgc ttttgttcca gttgtttcaa aatctcattg atatcttctt gagtcaaagt
    11641 ggcttcttct actagatccg caatcgtctc tccaatcctt tttgcacaga tatgggaaaa
    11701 aagattttcc gtcgcactgc gaactgtttc catctccgag acagctggat gataaatgaa
    11761 tttttttcct tcttgttctg tccagatcac ttcttttttt accaaacgac ccagcaaagt
    11821 tttgatcgtt gctggtttcc aatccatcga atcagctaag atctgcgtga tttcttgggc
    11881 tgtcgtctgt ccaagtgtcc aaataacacg catgatctcc cactctgaat cactaatctt
    11941 tactggcgtt ttgacttttt ccatttattt cacacctttc gtttacaaat gtaatcatta
    12001 acttatgaat acagattaca cttgtaaaca aaaactgtca attatttttg ctctctattt
    12061 tcaatttagt taaaaattct tgtttctctc gtcttgtttt aaggatgatc agtttatttc
    12121 ttgcattagc tacttggatc agctgctgta tttttggttc attgtttcct tcaaaatgcc
    12181 agataaaaga taaaaattct aagtatttcc tgtttagttt ctccgaaaat gattcaggca
    12241 tatctggccg agattttttg tttatctttc tttttagaga acgccaaact actcggtaga
    12301 tggcgagata acgcggcact ttaagccaaa tgatcttatc tgcttcttta atcctttcct
    12361 ctaacgtaga agtataattt ccttcaacga tccatgacgg attagatacc ataaacgatt
    12421 gctgttcttg caaaaaccac ttttttgcct gaatgcggaa agccggtgat ttctttgatc
    12481 ttccttgcga gggacgattt tcccgaccca ctttgtccaa tcacgatgat cttcattctt
    12541 ctccctcact tctctttgga atttggttcg atcataccac ttataacgcc gcaaaaaaac
    12601 aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaataaatgg tgttcaatca
    12661 gctgaccttc agcattagct caaaaaacgg caaaatatga ggagctattt ttgtttggct
    12721 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccagaa
    12781 gtagcttctg tcacaacctc ttgtaacaag tatttctatt ttaaggctga accactgttt
    12841 ctggttcagt aatttctttg tttttgtctc gacgagcaat tgctggcaag atcataccta
    12901 ataagatcaa gacaattggt gtgatgatat tcgtgaataa ttggaaccac catgctttag
    12961 gatcagcagt ataatctact tttggcacca ttcctaggat acatgcaaac gcagtgaagg
    13021 caaagcacca agcaccaaag ataaaaccga tttttggatt tttaacaaat ttatattcag
    13081 aattgaattt cttataagct ttattcaaca tcatatatgc caagaacacc cataggtaac
    13141 gcattggcat gacaactgag ttcaagttcg tcaaccattt tactaattca ttcatatctt
    13201 tgataccaaa gatcggcaac aaaataatga ttcctactag tatacctgtc agaagataac
    13261 cattcaccaa tgtaccttta cgattacgtt tacgtagcca gtttggtaca aattctggat
    13321 ccgcatctgc taacaagatt tttagtggtg catcaattga aaaggctaat gcggagattt
    13381 gtccaatcat gtttgtcaat gcgtaaatga tcatcaatga gttaccgata ccatagtaat
    13441 tacctaaaat ttgaaaagca ctgtatgccc catttgccat caagtcttta gggatattat
    13501 cgctagcaaa cagcataccc attgcgactg agcctaatac agcacaaatc cctaccattg
    13561 ctgctaagaa gatcatccct ttaggaaatt cctttgcagg atttcttgta gagttgacat
    13621 aaggggatat tttttccgcc cctccaactg caaatactag catcgaaacg gttgtgaaat
    13681 aactgaaatc aaatgttgga atatatgttt tcacttgatt catatgtggt gtggcaaatt
    13741 ccatcgttgg attgatgaac ggtgccgtta cagctaaaag aataaataag atcgacatga
    13801 cgaacatcgc tgttccggcc agtccgccaa tcactttcaa agtagaaagt ccttttgttg
    13861 ataaatataa aaaggcaaga aatataataa gtgaaataac tgctaccatt tgtactgaca
    13921 tcgtatttac gatatttcca tttccttgga ctgcccaacc accagcaatc agaattgctt
    13981 gaggcttttg tgccaaataa ggaatatgga cgacccagta agtccaagcg gcatagtatg
    14041 ccagtcgttt attagatgtc gtattttcga cccatgaact gacgcctcct ttactatcct
    14101 tgaaggctga tcctaattgt ccaacgatca atgcgtatgg aataaaataa atggccaaaa
    14161 tcaatagcca agaggtgaca actgatactc cttgctgagc atagttatta actacattcc
    14221 ccatgcccca aaccatgtta aatgcaatta acgcaacagt aaaccagcgt aattgtttgt
    14281 tgttcattcg ttctctcctt tatgataaaa atttgttatc cagatgtaat catattcaat
    14341 ttttaacaaa ctt
//
LOCUS       DAJSNV010000065        14221 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSNV010000065 DAJSNV010000000
VERSION     DAJSNV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.068"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(183..2180)
                     /gene="tkt"
                     /locus_tag="ON516_001925"
     CDS             complement(183..2180)
                     /gene="tkt"
                     /locus_tag="ON516_001925"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3345402.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2281..2532)
                     /locus_tag="ON516_001926"
     CDS             complement(2281..2532)
                     /locus_tag="ON516_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3345403.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2678..3301
                     /gene="lexA"
                     /locus_tag="ON516_001927"
     CDS             2678..3301
                     /gene="lexA"
                     /locus_tag="ON516_001927"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3345404.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3481..3699
                     /locus_tag="ON516_001928"
     CDS             3481..3699
                     /locus_tag="ON516_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345405.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3988..4500)
                     /locus_tag="ON516_001929"
     CDS             complement(3988..4500)
                     /locus_tag="ON516_001929"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3345406.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4514..6811)
                     /gene="recJ"
                     /locus_tag="ON516_001930"
     CDS             complement(4514..6811)
                     /gene="recJ"
                     /locus_tag="ON516_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3345407.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6953..7396)
                     /locus_tag="ON516_001931"
     CDS             complement(6953..7396)
                     /locus_tag="ON516_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3345408.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7397..8185)
                     /locus_tag="ON516_001932"
     CDS             complement(7397..8185)
                     /locus_tag="ON516_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3345409.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8182..9123)
                     /locus_tag="ON516_001933"
     CDS             complement(8182..9123)
                     /locus_tag="ON516_001933"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3345410.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9300..9887)
                     /locus_tag="ON516_001934"
     CDS             complement(9300..9887)
                     /locus_tag="ON516_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3345411.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10108..11415)
                     /gene="obgE"
                     /locus_tag="ON516_001935"
     CDS             complement(10108..11415)
                     /gene="obgE"
                     /locus_tag="ON516_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3345412.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11709..12719
                     /gene="gap"
                     /locus_tag="ON516_001936"
     CDS             11709..12719
                     /gene="gap"
                     /locus_tag="ON516_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3345413.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12804..13739)
                     /locus_tag="ON516_001937"
     CDS             complement(12804..13739)
                     /locus_tag="ON516_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3345414.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13925..>14221
                     /locus_tag="ON516_001938"
     CDS             13925..>14221
                     /locus_tag="ON516_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345415.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3974 a         3162 c         2510 g         4575 t
ORIGIN      
        1 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
       61 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      121 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      181 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      241 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      301 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      361 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      421 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      481 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      541 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      601 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      661 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      721 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      781 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      841 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
      901 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
      961 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1021 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1081 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1141 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1201 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1261 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1321 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1381 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1441 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1501 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1561 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1621 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1681 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1741 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1801 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1861 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     1921 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     1981 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2041 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2101 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2161 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt tacctttatt
     2221 tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca gtatttttta
     2281 ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt ttaatttttc
     2341 cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat gatgacgcat
     2401 accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt gttcttcggc
     2461 ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct ctattttttc
     2521 aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa aactgaaaaa
     2581 aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc tttttatatg
     2641 gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag
     2701 acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat acccgccgac
     2761 tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc acggtcatct
     2821 tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc gtgcaatcga
     2881 gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc cagtacttgg
     2941 cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact tctttccaat
     3001 ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc gtggagatag
     3061 tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc aagaaactgc
     3121 tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt
     3181 ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg aaccaatcat
     3241 cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc atatctatta
     3301 attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt ctcaaactct
     3361 tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta caaaccgaac
     3421 aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag gtgagaattc
     3481 atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg tattattatt
     3541 ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac tttgtggttc
     3601 atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca tgaagatcat
     3661 ttctatcatg agccctacta cagatccagg caatcataat aaggattgac tgctaattat
     3721 tcaaaagaca agaagttaaa cttttacggg ggtgtgacaa aaatcggtca catccttttt
     3781 ttcgaataaa cggtgttcta aagccagttt ttcggtatta agtcaaattg cggaaaaagt
     3841 gattttttaa acttctactg gtctttgctt gtttccattt ataaaaaaat agcgaccctc
     3901 aaaagtcaga tagagaatct gacttttgag ggtcgcttta aaatcgcttc tttaagaata
     3961 acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga tgtcatatcc
     4021 tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc agcctacgac
     4081 tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc cagtagctag
     4141 caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat gcaatgtcaa
     4201 tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag gtaacttccc
     4261 ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac atcctacgat
     4321 gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg cataatttac
     4381 gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg aaatatctcg
     4441 aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt ttaaatccac
     4501 tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg ctcaaaagca
     4561 aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc tcagtcaaag
     4621 gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta acaaatttca
     4681 attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc aaatattgag
     4741 ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc aatttaaaca
     4801 attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc
     4861 ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga tccgctaaat
     4921 catctggaca atccataaca actagagagt ttggttttct tttttcaatt attgccttta
     4981 cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc acgaatttca
     5041 acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc tgttcacgga
     5101 aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca gataccatca
     5161 gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc acatctacct
     5221 gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca tcaagctgac
     5281 tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt cttttgatat
     5341 tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta ccaaatggcg
     5401 ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc aatacttcat
     5461 caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata tattggttca
     5521 tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact gctgcatgat
     5581 gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa agattcaagg
     5641 cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta aggacaagcg
     5701 ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg ccttcatgcc
     5761 atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct aatgcttctt
     5821 gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat ttttttgctt
     5881 gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga cgtggatcgc
     5941 ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt gtttcgtctg
     6001 cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc cgttctgaat
     6061 gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta
     6121 aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt tcttccaata
     6181 atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat ggatagtgcc
     6241 ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca ggtagttcgt
     6301 gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc gcatcatttc
     6361 ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc ttttcttggt
     6421 ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat acttctactt
     6481 ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc ccatctgcgt
     6541 cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg attcggctga
     6601 ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc tttggatgta
     6661 aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac ttaccgacaa
     6721 atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc tctgtctcgc
     6781 ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag ttattatagc
     6841 aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga tccgctagac
     6901 agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc tattagtcaa
     6961 aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct ttcaattgac
     7021 tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac tccgcacgtt
     7081 cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt tgaagttctg
     7141 tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc tgccatagtg
     7201 ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg atgatcacta
     7261 agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt ttattgttca
     7321 atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca ttgctttgct
     7381 ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa tcaccaatcg
     7441 ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga ttgatttccc
     7501 gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt tctaaaacga
     7561 tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag aaatttgtct
     7621 cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat tctaatcgca
     7681 aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt gattttgcag
     7741 ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt ctttctgcca
     7801 tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact tcaaacatat
     7861 cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt
     7921 ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat tcgtccacgc
     7981 tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc aagcgtctgc
     8041 actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata acgattgctc
     8101 cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta cctgttacta
     8161 aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct
     8221 atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt tttcaagctc
     8281 taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc gttttgcctg
     8341 cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat
     8401 tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat tttccgcaag
     8461 cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc gtcctttttt
     8521 atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt ggatcgtttc
     8581 accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt ttagacgatc
     8641 cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac tgtcgatccc
     8701 atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc
     8761 atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac ctaaagagac
     8821 ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat ccccaccttg
     8881 aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt
     8941 aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt
     9001 gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt tcaatgctat
     9061 acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa attgtaattc
     9121 cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa gaagggataa
     9181 tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt
     9241 ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc tgttttttat
     9301 taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt gtccttccaa
     9361 gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg cacatctttt
     9421 tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat taccaattga
     9481 ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc tccttcaccg
     9541 attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt ggcatcttgt
     9601 gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg accacctaat
     9661 acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc ggtaaagtcg
     9721 ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac aactgccatc
     9781 tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa tgcctgatat
     9841 gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa aaacgcccct
     9901 ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag aaaagcactc
     9961 tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt tttccgaata
    10021 aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg aatctatatt
    10081 ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa actcaccgat
    10141 acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc
    10201 acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg tcatttggaa
    10261 taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac gatcgatttg
    10321 gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca gttcttcttc
    10381 gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca ataacgcgtc
    10441 caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag caaattcatc
    10501 ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg catccggcat
    10561 atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt tataagtaga
    10621 taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca tcccactcat
    10681 atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag
    10741 cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg caaaacttct
    10801 gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc
    10861 gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga cagatggaaa
    10921 acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc gctcttgtcc
    10981 tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa aacggatatt
    11041 ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt caattaaatc
    11101 tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta ctttaacata
    11161 ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac ctggctgcgc
    11221 tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat cgacgatcaa
    11281 gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag gaacgtattt
    11341 ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat cgatcgttac
    11401 ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt atttgactgc
    11461 ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct gattttgtct
    11521 atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt aaggtttcca
    11581 accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt agcagaataa
    11641 cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa
    11701 cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt
    11761 tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa
    11821 gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca
    11881 cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag
    11941 aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat
    12001 gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac
    12061 gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg
    12121 atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag
    12181 cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag
    12241 ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc
    12301 gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa
    12361 tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag
    12421 tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc
    12481 aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc
    12541 gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc
    12601 aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca
    12661 atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat
    12721 ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt
    12781 tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact
    12841 tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca
    12901 tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca
    12961 aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc
    13021 actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact
    13081 tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca
    13141 tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct
    13201 ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc
    13261 gattttccat tgttttctag cactttgttt atccactgta catagtcttt acgctctggc
    13321 catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg
    13381 acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc
    13441 atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc
    13501 gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa
    13561 ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg
    13621 tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac
    13681 actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata
    13741 aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct
    13801 ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta gaaaaataaa
    13861 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
    13921 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
    13981 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
    14041 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
    14101 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
    14161 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
    14221 g
//
LOCUS       DAJSNV010000066        14059 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSNV010000066 DAJSNV010000000
VERSION     DAJSNV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.069"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..990
                     /locus_tag="ON516_001939"
     CDS             <1..990
                     /locus_tag="ON516_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3345416.1"
                     /translation="AMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHNRI
                     YKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELIST
                     NVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKW
                     SAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQ
                     QDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSN
                     ASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1564..2133
                     /locus_tag="ON516_001940"
     CDS             1564..2133
                     /locus_tag="ON516_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3345417.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2120..2980
                     /locus_tag="ON516_001941"
     CDS             2120..2980
                     /locus_tag="ON516_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3345418.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3170..3874
                     /locus_tag="ON516_001942"
     CDS             3170..3874
                     /locus_tag="ON516_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345419.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3879..5714
                     /gene="walK"
                     /locus_tag="ON516_001943"
     CDS             3879..5714
                     /gene="walK"
                     /locus_tag="ON516_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3345420.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5711..7027
                     /locus_tag="ON516_001944"
     CDS             5711..7027
                     /locus_tag="ON516_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345421.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7028..7897
                     /locus_tag="ON516_001945"
     CDS             7028..7897
                     /locus_tag="ON516_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3345422.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7952..8761
                     /locus_tag="ON516_001946"
     CDS             7952..8761
                     /locus_tag="ON516_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3345423.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8864..9493)
                     /locus_tag="ON516_001947"
     CDS             complement(8864..9493)
                     /locus_tag="ON516_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3345424.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9663..10955
                     /locus_tag="ON516_001948"
     CDS             9663..10955
                     /locus_tag="ON516_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345425.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11031..11693)
                     /locus_tag="ON516_001949"
     CDS             complement(11031..11693)
                     /locus_tag="ON516_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3345426.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11918..12202
                     /gene="groES"
                     /locus_tag="ON516_001950"
     CDS             11918..12202
                     /gene="groES"
                     /locus_tag="ON516_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3345427.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12253..13878
                     /gene="groL"
                     /locus_tag="ON516_001951"
     CDS             12253..13878
                     /gene="groL"
                     /locus_tag="ON516_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3345428.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4732 a         2374 c         2984 g         3969 t
ORIGIN      
        1 gctatggaat gtgaatgtgc taatccaaat ttattgtttt atgactattt gcttaattat
       61 ttggaagtat caaaaaaaca agtagcattt aacacatatg ttgggtataa gcattacgtc
      121 cataatcgaa tttacaagtt tttttatcct aaaaaaatta aactaaatag gttaaagcct
      181 tatcatttac aagagtttta tcaatatatg ttaaatgata actgtacagc aaatacggtt
      241 attcattatc acgcatttat acgcaaagct cttcaagagg ctgttattac agaattgata
      301 agtacaaatg tagcagataa agtaagaaag ccaaaaaaga aacaatttgt ttctcaagtt
      361 tataatcaac aagagatctt gaaattactt gacattttgt cagaagaaaa actctatttg
      421 gtagttcttt taacagcatt ttatggattg cgaagaagtg aagttttagg attaaaatgg
      481 tctgctattg attttttaaa caaaaaaatc atgataaacc atgtaatcat agaaaatcca
      541 gagaatatgt cacagttaat aaagaaagat caaacaaaga actcttccag ctatcgtaca
      601 ttacctttgg ttgaaacgat tgaacgagca ttagtaaatc aagctaaatg gcaacaagat
      661 aatcgtattc ttttaggaga tgattatatt ttagaagata atgagtacgt atttactatg
      721 gaagatggac ggttgatgaa gcctcaatat gtaacgcatc gtttgagtaa gttgattaag
      781 aaaaatggat tgaaaaaaat tcgctttcat gacttgcgtc attctaatgc ttcaataatg
      841 ttagatagtg gccagaatat gaaatccatt caagaatggc tgggacatgc atcgtattcg
      901 acaactgcta atttatacac gcatttaacc gcaggagtaa aagatcgtgc agcagaagca
      961 ttagaaaacg catttgggtt taaagaataa aaaaacaaaa aacaatatct ttggggtatc
     1021 gcttcttgtt tttagaattt ttttgttggc aatttgttgg cagtatgttg gcaatgaaaa
     1081 aattcacaaa ctaaaaacct atttataaag ctttataaaa cttttttcac aaatagagca
     1141 tttggttaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
     1201 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
     1261 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
     1321 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
     1381 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
     1441 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
     1501 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
     1561 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1621 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1681 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1741 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1801 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1861 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1921 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1981 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     2041 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     2101 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     2161 ggatgtgcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     2221 ttataaagat caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg
     2281 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     2341 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     2401 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     2461 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     2521 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     2581 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2641 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2701 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2761 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2821 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2881 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2941 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     3001 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     3061 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     3121 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     3181 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     3241 aggctatgat gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     3301 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     3361 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     3421 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     3481 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     3541 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3601 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3661 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3721 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3781 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3841 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3901 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3961 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     4021 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     4081 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     4141 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     4201 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     4261 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     4321 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     4381 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     4441 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     4501 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     4561 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4621 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4681 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4741 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4801 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4861 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4921 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4981 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     5041 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     5101 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     5161 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     5221 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     5281 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     5341 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     5401 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     5461 acgtggtttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     5521 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     5581 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5641 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5701 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5761 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5821 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5881 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5941 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     6001 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     6061 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     6121 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     6181 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     6241 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     6301 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     6361 actgaaaaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     6421 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     6481 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     6541 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6601 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6661 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6721 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6781 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6841 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6901 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6961 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     7021 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     7081 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     7141 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     7201 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     7261 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     7321 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     7381 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     7441 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     7501 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     7561 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7621 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7681 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7741 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7801 cgaaacaagc gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt
     7861 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7921 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7981 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     8041 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     8101 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     8161 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     8221 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     8281 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     8341 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     8401 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     8461 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     8521 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     8581 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8641 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8701 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8761 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8821 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8881 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8941 ctgccatgct gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca
     9001 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     9061 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     9121 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     9181 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     9241 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     9301 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     9361 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     9421 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     9481 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     9541 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9601 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9661 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9721 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9781 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9841 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9901 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9961 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
    10021 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
    10081 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
    10141 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
    10201 cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat
    10261 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
    10321 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
    10381 ggttggttag tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag
    10441 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
    10501 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
    10561 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10621 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10681 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10741 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10801 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10861 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10921 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10981 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    11041 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    11101 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    11161 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    11221 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    11281 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    11341 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    11401 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    11461 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    11521 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    11581 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11641 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11701 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11761 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11821 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11881 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11941 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    12001 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    12061 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    12121 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    12181 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    12241 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    12301 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    12361 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    12421 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    12481 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    12541 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12601 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12661 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12721 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12781 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12841 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12901 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12961 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    13021 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    13081 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    13141 acagtaatta ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga
    13201 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    13261 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    13321 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    13381 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    13441 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    13501 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    13561 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13621 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13681 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13741 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13801 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13861 atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag
    13921 acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg
    13981 gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
    14041 ccgctgttta tacgtgttt
//
LOCUS       DAJSNV010000067        13687 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSNV010000067 DAJSNV010000000
VERSION     DAJSNV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.070"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..447
                     /locus_tag="ON516_001952"
     CDS             <1..447
                     /locus_tag="ON516_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345429.1"
                     /translation="AVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVHEDKV
                     KDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLPYQVA
                     DDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            469..699
                     /locus_tag="ON516_001953"
     CDS             469..699
                     /locus_tag="ON516_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345430.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            1167..1475
                     /locus_tag="ON516_001954"
     CDS             1167..1475
                     /locus_tag="ON516_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345431.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            1477..1899
                     /locus_tag="ON516_001955"
     CDS             1477..1899
                     /locus_tag="ON516_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345432.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            1919..3508
                     /locus_tag="ON516_001956"
     CDS             1919..3508
                     /locus_tag="ON516_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345433.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLNEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            3520..3855
                     /locus_tag="ON516_001957"
                     /pseudo
     CDS             3520..3855
                     /locus_tag="ON516_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4003..4197
                     /locus_tag="ON516_001958"
     CDS             4003..4197
                     /locus_tag="ON516_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345434.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEVESERTFVVTDKEAKAYQPGDL
                     LTLDLTTKKFVDAAENYPFI"
     gene            4606..6993
                     /locus_tag="ON516_001959"
     CDS             4606..6993
                     /locus_tag="ON516_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345435.1"
                     /translation="MPEEVKGKSSLTLNEEEITGNSRLAQAQRKLIRLRNEYDATFER
                     LAEHQKLTNGQPMNDKRNGSSWFSQREKIENKINQLQEEIQKQENYVEKLTRQSELRK
                     MGYNKYGGLDLTIENIPRIKEEIEKFRNGKSQFSVKTIEKYQKKLVELEQLKEKSEKG
                     IANILPEAQAIIESGRVSQWKRNPTIYFVKGYRKVALELNEQGEFEVSKRYQPKTEEE
                     KRIVSELLENKMDEYGVQKQEPNHYYRVEFNENDGTMRNYQGEIVTQSLLEQIKALDT
                     HYIGKTSYHKFYFEEVKEGEVVNQLRLDVGDGLQLNASIYQKLEELCDQTLETKTEKV
                     IKNEIGSHDKKVETKKPSYQKRSTEEIKQEVKLLSQQALDAVKKYTHSPEDVKELLNF
                     MSRFPERSFRNQLLIEKQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYD
                     KGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRV
                     FDFQLDKKGQAELQAGIQAVANTIGIQIRDMTESEVYQRELGQARGAYVHNEFTGQEE
                     IVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEK
                     AIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPA
                     EVKQEHKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            7054..8514
                     /locus_tag="ON516_001960"
     CDS             7054..8514
                     /locus_tag="ON516_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3345436.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRKKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYILKDESKVERVEN
                     LHFETQEYKHHPMDYQPLTEKGRTYLKEQRKFPEWLIEYGDQQGLILEMKPRSERKNY
                     IVKDDRLDYAIVFPWKDRHTDQVLGATYQGTIIDYERFGDRGTYKHIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYVALNRETVKDTWLISMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHIFWDKVHRLGVSNPFTGKAVYFEPDLPNDWQVPRKYQTI
                     YEEVGKEMNVAPEAIMAIHKIENNLTENNQIVLFGEIHGVFAKKLASKEKVQIIDVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEEIQISLKAKHYFEGYKNHDHEFVSE
                     VKKDWNDQWKHEIQQQEIRKQKRAMLFQQSRQQERE"
     gene            8525..10729
                     /locus_tag="ON516_001961"
     CDS             8525..10729
                     /locus_tag="ON516_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR3345437.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIHDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWSIIQQAGADQGKKFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKTQVTAQNGTFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            10750..10899
                     /locus_tag="ON516_001962"
     CDS             10750..10899
                     /locus_tag="ON516_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345438.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            10975..11568
                     /locus_tag="ON516_001963"
     CDS             10975..11568
                     /locus_tag="ON516_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3345439.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            11584..12483
                     /locus_tag="ON516_001964"
     CDS             11584..12483
                     /locus_tag="ON516_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3345440.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            12486..12977
                     /gene="ssb"
                     /locus_tag="ON516_001965"
     CDS             12486..12977
                     /gene="ssb"
                     /locus_tag="ON516_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3345441.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRTHTDQSSTSTSPSTTAFEQHEKLPTNNNGFNDSQNPFGGQSIDISDDD
                     LPF"
     gene            complement(13118..13321)
                     /locus_tag="ON516_001966"
     CDS             complement(13118..13321)
                     /locus_tag="ON516_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3345442.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            13396..13656
                     /locus_tag="ON516_001967"
     CDS             13396..13656
                     /locus_tag="ON516_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345443.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         5299 a         2142 c         2779 g         3467 t
ORIGIN      
        1 gcagtgaagc taacgcatgt accagaagaa tatatcgcca tgcgtttaac cattattcct
       61 gaaaaaattg atccaaaagt ggatatgcaa gaaccacaag atctgattta ttacgtgcac
      121 gaagataagg ttaaagatca agtgaaagat gaagattatg aacaacacgc gatcaattac
      181 aaagtgaaag gatacaagaa agaacaaaaa gcagctcaaa aacaaatgga atctttccaa
      241 gcaaccattg atttgaatga agaattagtg aagaagctaa aagatcaatt gccttatcaa
      301 gtcgcagatg atcaggaaaa tactgaaaac aagattaata gctaccagca agaaattgaa
      361 acatcgaaaa cgcaactgaa agatcaagaa gagatcattc aaaaacttca agaaaaaatt
      421 gatcgtatga caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat gaagcgaaca
      481 aacttggctg acaaagatac tttcgctgag caaaaagtat tgattaaagc ctatctcaaa
      541 gttgtaaaag ctaaagaaat atgtcgagaa gtgaatttat ctaacacaac aatatctgag
      601 ttgaagaatg gtcgaaagaa tttagatact ttaaaattca gatcgtttga agctctatat
      661 gtagctgcta aaaaatattt tacggctgaa aggaaataaa aacaggagga ttttatgtga
      721 aaaaagtata tcaaaaatgt ttatggatca gctatatcac cgtaacacaa acatcaagca
      781 cataaaacat ttaacactcc tcctttctgg ctgaagattt caaccaaaca ggaagacagc
      841 gtatcaatta tagcatacac tttttcttaa attcattttt catatgacaa aatatttcct
      901 tctaatcgac aaagttagaa ggatttttta ttgtctaaat acacaaataa ccttaatttt
      961 taaagagcat aaggaaacgt gaccccactt ttaaaaaaag tggttggata ggctgtgcct
     1021 atcacgtttc tgaaaaaaat aaatgacccc aaaataccaa tatataggat agtacaagga
     1081 tagcaaagga tagtaaaagg atagaggtag gatagtgaaa aggataggct ccttttcact
     1141 atcctatatt atattggagg atatttatga atgttaaaat tagagattta gatccaaaat
     1201 tcatttctga aattgatcga agatgcgttg aactaagtaa tcgtacagga aataaatgga
     1261 gtcgtaatga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct ttgatggaat
     1321 ataaacaaga taaatttgat caattattaa atcaattaat agataaattt tcttttgttc
     1381 aaagtcataa tacaaaagta ttagaagaat atatttgtca gaataatcaa attatcgaac
     1441 ttttaattga acaaagtaga ggaggagaag tttaacatgg ccaaagttat agacaagcat
     1501 atttattttt atgaaacaca tatagatgtt ctagatgaaa ttattgcaac tcatgcagag
     1561 gttaaaaact atagtgaagc cgttcggttt ctatgtatga attatgatcc agaagtggag
     1621 aaaaaagtaa attccaatca agcaaagttg aacggaatga gtaaagaaat ttctatttta
     1681 accgaaatgg tttcagagtt tgcggatctc catttgaaag atacagggat tttaattggt
     1741 acaaattcaa ctgtttacca agaagcaaaa gagcgtgtgg aaggcaagat aaaagcaaat
     1801 caaacgaaga aatattctcc taagataaag aaaacaataa gcgaatcagt gactacaaaa
     1861 agtgaagtat ttgatccaac aaggttctta aaacgataaa aattagaagg agaatgtgat
     1921 gagtaaatca cctgcagtca ttctcacttg tcaatttacc ttgcccaata aaaaatcttt
     1981 ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg aacgacgtac
     2041 gcccgaggaa gaacaggaac tgcgtctaat caagaatgct ttagaggatt tttatattcc
     2101 tgaaggagaa actttttcgg agagtaagac ggataaagaa ttcagcgaaa cgtcgaatga
     2161 agctcaatct ttgatgggaa atggaatcaa ctttgacaag ttagaggaac aagattttac
     2221 caaatatctt tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca
     2281 aagtgaacaa aaagaatacc aacgcttgaa ccaagcaata aaaaaatatc aaacagagcc
     2341 acacaaaaaa gcttcaggaa accttcctgg agttttttct atggcttcag acgaagtgaa
     2401 aggaagtgat ctgaaggaga ttaagcaaat cttccaaaga ggacaacaaa aaggctcaat
     2461 catttatcaa gatgttgtct ctcatgataa ttcgtatctt gaaaaaatag gattgtacga
     2521 tcccaaaaca gatactttag atgaagaagg attaaaagca gctggaagaa aaatgatgga
     2581 gacattattc gagaaagagc aactgaatga aacaggctac tggatggcaa cgattcaccg
     2641 aaataccaaa catatccata ttcattttgc agttgttgaa aaaaaaaata cgcgtaaacc
     2701 atacactgag attgaaaatg gtgtgagtta tatcgtaccg aaagggaaga gaaggcaagc
     2761 aacattagac gaaatgaaga ctaattatgt acatgagtta gaacgttttt cttatgaaaa
     2821 aaatcgaatg ggtatggaga aacgaaattt attgacacgg aaaagtgatt tgcgtaacac
     2881 cttaaccaaa gtagtgaaag agccagtacg ttatgatcga cacgcaatga gcttattaaa
     2941 tgaagtatat aaaaacttac ctaaaaagcg ttcggaatgg cattacgggg atgaaaatcg
     3001 aaccaaactt tcaccataca caagagagaa actcgatgag ttaacccaat atgtgttaac
     3061 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
     3121 aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga
     3181 tttgaagaag cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat
     3241 aaaaccttta atagaaagga tggatcagat gcaatctatt tacaatgaac gatcctttcg
     3301 aacacttaaa cagttgccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
     3361 caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
     3421 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
     3481 aattcaaatg gaacaagaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa
     3541 tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta
     3601 taaaaagagc atttgtttca gataagagaa atgttgagaa acgaacagtt tgatccaaat
     3661 caatttttag tcgttgagta cccagctact ggtgtgtaat gttgtaatca tgtgaagggt
     3721 gaaaaatatt ttatcattca aaaatatgag ggcaagttag cgccttatta tactacttgg
     3781 gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca
     3841 ctcacggaat gttaaataaa tagagaaata aaagagtcct gaacttgatt gtccagggct
     3901 ctttttaagt gaattttttt ccgtcaattc actgagtggt gcgattcaat tttattatat
     3961 caaaagaaag tgagattcca aagagaaggg ggggaagaag acatgactca gacaacgatt
     4021 ggagttgttg ttgaaaaaag cagagacaat ctagtgtttg tgacagaagt cgaatcagaa
     4081 agaacatttg ttgtcacaga caaagaagca aaagcgtatc aaccagggga tctcttaaca
     4141 ctagatctca ctacaaaaaa atttgtcgat gctgcagaaa attatccttt tatttaagaa
     4201 aagatggtga acaaaatgga acaatttcaa caagtaaatc ctgaagtaca agaacaactg
     4261 aagaaatggg aatcactttc acgtgaagca caaaatgaaa ttatagtgat cttttctcaa
     4321 aatcctgtta atttcagaat tgctttaatg aaatatgatt taaaggatac tgaaaatatt
     4381 tttacaggat tgggtacgcc tatagggaag gcttgggaag aacgatacaa agagttagaa
     4441 aaagaagtta gtaccttcaa agcaacccgt agagaatggt tagatcaata tgaagatagt
     4501 ggggttatgt atattccaga aagtttatgg gagacagctg aagagttagg aataatgaaa
     4561 aactacccca aagtagttcc aactagcgaa ctcactgcac cagttttgcc agaagaggta
     4621 aagggaaaaa gtagtctcac tttaaacgaa gaagaaatca ctggaaatag tcggcttgca
     4681 caagcacaaa gaaagcttat ccgattaaga aatgagtatg acgcgacatt tgaaaggctg
     4741 gctgaacacc agaaattaac aaatggtcag ccaatgaatg ataaaagaaa cggatctagc
     4801 tggtttagtc aaagagaaaa aatcgaaaat aaaattaatc aattgcaaga agagattcaa
     4861 aaacaagaaa attatgttga gaaattgact agacaatctg agcttagaaa aatgggatat
     4921 aacaaatatg gtgggctcga tcttacaatc gagaatattc cacgaatcaa agaggagatc
     4981 gaaaaattta ggaatggaaa atctcaattt tcagtaaaaa caatcgaaaa gtatcaaaaa
     5041 aaattagttg aattggaaca gttgaaagaa aaatcagaga aaggaatagc caatattttg
     5101 cctgaagcgc aggcaattat tgaaagtggc cgtgttagtc agtggaaaag aaatccaact
     5161 atatactttg ttaaagggta tcgtaaagtg gcattagagt tgaatgaaca aggcgaattt
     5221 gaagtatcaa aaagatacca accaaagact gaagaagaaa aaagaattgt gagtgagtta
     5281 cttgaaaata agatggacga atatggtgtt caaaagcaag aacccaatca ttattatcgt
     5341 gtagaattca atgaaaatga tggaacaatg cggaattatc aaggtgaaat cgtgacacaa
     5401 tcactacttg aacaaatcaa agcattagac acacactata tagggaaaac aagctaccac
     5461 aaattttatt ttgaagaagt aaaagaaggt gaagttgtta atcagttacg actagacgtt
     5521 ggagacgggt tacaattgaa tgcttctatt tatcaaaagc tagaggagtt atgtgaccaa
     5581 acattagaaa cgaaaactga aaaagtaata aaaaatgaaa taggtagcca cgataaaaag
     5641 gtagagacaa agaaaccaag ttatcaaaaa cggtcaacag aagagattaa gcaagaagtc
     5701 aaattgcttt cccaacaggc actagatgcg gtgaaaaaat atacccattc tcctgaagac
     5761 gtcaaagaac tcttgaactt tatgtcgcgt tttccagaac gatcttttag aaatcagtta
     5821 ttgatcgaaa aacaatttcc aggagcaacg gcgtgtttag gtcgtgctgc gttgaaaaaa
     5881 gaaggcattt atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     5941 aaaggattct atgataaagg acggggcttt gttcgagaaa cagaggctac accagaagat
     6001 aaaaaacgaa tcgaaagtgg ccaaatcgaa gtgatcaaaa aaccttacta cacgattgaa
     6061 aaagtgtttg atatcacaca aacacagttg aaacctgaag actatccgaa aatcttcccg
     6121 aaccgtgtgt ttgattttca attggataaa aaaggacaag cagaactaca agctggaatt
     6181 caagcggtag ccaacactat tgggatacaa attcgagata tgacagagag tgaagtctat
     6241 caaagagaat taggacaagc acgtggggcg tatgttcata acgaatttac tggtcaagaa
     6301 gaaattgtca tgaatactag aaatacaccg acgcagcatt tagctacaag tattcacgaa
     6361 ttagcacatg ccagaatgca caaattttca gaattagaca ctgcgaccaa agaactgcag
     6421 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     6481 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     6541 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     6601 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     6661 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     6721 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     6781 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     6841 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     6901 ttccgatttt cacttgagac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     6961 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc aagaaagact atctgaaaaa
     7021 tggatagtct tttttctttt gggaggtttg aaaatgacag agaaaaaaat gagtcttatt
     7081 gatcgatgca agcaaatgga tattgtagat tttgctcgaa acaatggttt agcggtagtc
     7141 aataaaggac gagactatcg tttagaagac catgattctt ttgttttcga tcgaaaaaaa
     7201 cagcgatttt attggaacag tcaagggatt ggtggagata ttattgaatt gactcaattg
     7261 ttttttattg acgattctat tcaaaaccca aagcaacgtt ttaaagcggc gctagattat
     7321 attttaaagg acgaaagcaa ggtagaacgg gtggaaaatc ttcattttga aacacaagaa
     7381 tacaagcacc atccaatgga ttatcaacca ctcacagaaa aaggacgaac ttacttaaaa
     7441 gaacaacgaa aatttcctga atggttgatt gaatatggag atcaacaagg gttgatcttg
     7501 gaaatgaagc cacgaagtga acgaaaaaac tatattgtga aagatgatcg tttagactac
     7561 gcgattgttt ttccttggaa agaccgacat acagatcaag tattaggagc aacttatcaa
     7621 ggaacgatta ttgattacga acgttttggt gatcggggaa cgtacaaaca tatcgatatc
     7681 aatcctactc ctaatcacgg atttaatgtc cttcttggtt ctcctaaaaa tttaaaattt
     7741 tttgagagtc ctattgattt attgagttac gttgctttaa atcgtgagac agtcaaagat
     7801 acatggctca tttctatgga cggactgaag gatgcagtag taggacacta tgttcgagaa
     7861 gcggtaacgg aattagcgcc gaaagaggaa tttccagaat cgatcgagat ttgtgtggat
     7921 aatgatcgag ctggacacat attttgggat aaggtacacc gactgggagt gtcgaatcct
     7981 tttacaggaa aagcggtgta ttttgaacct gatcttccca atgattggca agtaccgaga
     8041 aaataccaaa ctatctacga agaagtaggg aaagaaatga atgttgcacc agaagcgatt
     8101 atggctattc ataaaatcga aaacaatcta acggagaata accagattgt attattcggt
     8161 gaaattcatg gagtatttgc caaaaagctt gcgtcaaaag agaaagttca gatcattgat
     8221 gtgaaagaga aatgtataga agccgctcag cagttaaaat cgtgtgaaaa atctgatgga
     8281 acgtacgatt ttgatcgctt ttatcgtgga aaagggaaaa ttaatgaaga aattcaaatt
     8341 tcattgaagg ccaaacatta ttttgaaggt tataaaaatc atgatcatga atttgtttct
     8401 gaagtgaaga aggattggaa tgatcaatgg aaacatgaga tccagcaaca agaaattaga
     8461 aagcaaaaac gtgccatgtt gtttcaacaa agcagacaac aagaaaggga gtgaaagaag
     8521 cacgatgaaa actgtcatat tagcagaaaa gccgtcacaa gcgaaggctt atgccgatag
     8581 cttctctaaa gccacccgaa aagacgggta ttttgagatt catgatcgat tgtttcctgg
     8641 tgaaactgtg atcacctatg gttttggtca tttggtcgag ttagattcgc cagatatgta
     8701 cgatgaaaat tggaaacaat ggtcattaga acatttgcct atttttccta cacattatcg
     8761 ctatcatgtt cctaaagata agaaaaagca atttaacgta gtgaagcagc aacttcaatc
     8821 ggctgatacc attattattg cgacagatag cgatcgtgaa ggagaattga ttgcttggtc
     8881 gatcattcaa caagcaggcg cagatcaggg aaaaaaattt aaaagacttt ggatcaattc
     8941 attagaaaaa gaagcgattt atcaaggatt tcagcagtta cgagacgctg gagagaccta
     9001 tccaaaattt gaggaagctc aagcacgtca aatcgctgat tggttgatcg gtatgaacgg
     9061 tagtccacta tatagtttac tcttgcagca aaaaggcata ccaggaagtt tctccttagg
     9121 cagagttcag actccaacgc tttatatgat ttatcagttg caagaaaaga ttagaaactt
     9181 caagaaagaa ccctactttg aaggaaaaac ccaagtaacc gctcaaaatg gcacatttga
     9241 tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa gcatttgaag cttatttaaa
     9301 agaaaaaggc gttcaagtag gaaaacagcc aggcacgatt cttgaagtcg aaacagagaa
     9361 aaaaagcgcg gcaagtccgc gtcttttttc tctatcgagc cttcagtcta agatgaatca
     9421 actcatgaaa gctagtgcca aagacacatt ggaagccatg caaggattgt atgaaggaaa
     9481 gtacctaagc tatccacgaa cggatacccc ttatattaca gaaggagagc atgcgtatct
     9541 gttggatcat ttagatgaat acaagcattt tttaaaggct gaatcattcc ctactcctat
     9601 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     9661 tcctacaaaa acagttatga cagaagcaac gtatgaaaag ctgacacctt tacaacaagc
     9721 gatttatgat caagttttaa agacgacggt ggccatgttt gcggaaaaat atacctatga
     9781 agaaactacg attttgacac aagtccagca gcttcagcta aaggcagtcg gaaaagtacc
     9841 agtagatcta ggttggaaaa agctatttgg caaagagagc gacagaaaag agaaggaaga
     9901 agaaccgcta cttcctaaag taacaaaagg agaaacggtg acggtagatc tacaaatctt
     9961 tgaaaaagaa accaagccac cacaacctta cacggaaggt acactaatca ccgccatgaa
    10021 gacagctggt aaaaccgtgg atagtgaaga agcacaatcc atattgaaag aagtggaggg
    10081 aattggtaca gaagcgacac gagcaaatat tattgaaacc ttgaagcaaa aggaatatat
    10141 caaggtagag aaaaataaac ttgtggtgac gaataaagga attttgcttt gtcaggctgt
    10201 tgaaaaagaa ccgctgttaa caagtgccga aatgaccgcc aaatgggaaa gctatcttac
    10261 aaagattggt gagcaaaaag ggacacaagc tacttttcta gcaaatatcc aaaaatttgt
    10321 ttcccacttg ttggaagtcg tcccaggaca aatccaagcc actgattttg gagcaacgtt
    10381 acaagaggtg aaagtagcca gtgaaaagca agaagcggcg cgtcatttag gatcttgtcc
    10441 aaagtgccag gaaaaagaag tcatacttta tcagaaggtg gcgacttgca cgtctgaagc
    10501 ctgtgatttt aaattgtggg taacgattgc caaaaagaaa ctcacagcga cacaactgaa
    10561 agagatcatt cagaacggcc gaacaagtca accagtcaga ggactaaaag gtcagaaagg
    10621 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggatttg agttttctga
    10681 aaagaagaag accacctaca aaagaaaagt gagacgtaaa acgagttaga attgacggag
    10741 ggaaaaacga tgagaaatac aacgcataaa gaacaagaaa tcgaaaatca aaaagccaaa
    10801 atacgttcag agcaggagaa aatgcgtgcc agcaaacgaa gactacagca attgaagaat
    10861 caacaacgga ttcaaaaaga acttgaatta gatcgataaa tcctcctgtt gttaatatta
    10921 aactaacatg ttatagttat gttagtttaa tattaacatt cgagagggaa agcgatgaaa
    10981 gataaagtca agtgcatttt tgagaaatac aatggtaatc tatccttgaa ggatgctaga
    11041 aatgaagggt tatcacctat gacgttagat cgtttagccg aaaaggacaa aatagaaaaa
    11101 atagcatcag gtttttatac cttaaaagat agttttgtgg atgaattata tctcgctcaa
    11161 agtatctata ctagaggaat cttctctcac gagacagcgc ttgatctttt tcaagtagga
    11221 acatatatcc ctaaaaaaat taatatgatg tttcctaaag gatacaatgt ttcgaaagag
    11281 aatttagatc agtatgccat tcaaccgcat tacacgaata aagaaaactt tgaactggga
    11341 atcacagagg cagaaagctt ttacggcaat aaaattttcg tctatgattt agagcgaacg
    11401 ttatgtgata tgtggaatcc cagatataaa gcaagcgtag aagtaaaaca agaagcatta
    11461 aaagaatata tgacaagtac cagcagaaat acaaataagc taagaagata catgaatatt
    11521 ttaaaatcac caaaagaaat gactttgtat atgttgccac tttattgaga ggagtaaagg
    11581 tgaatgacac cgacacaatt taaagcgaaa acaagaaatg ttgcagctga gaaacattta
    11641 aacccacaaa ttgttcaaag atattatatg atggaaaaat tattagaatg tattgttgaa
    11701 tcgccttatc atgatgattt tgtattaaaa gggggatttc tgatcggatc aaaatacggg
    11761 ttagaaaacc gttcaaccgt tgatattgat acgacttatc gaaattcgaa actaacaaaa
    11821 gaaaaattag aaactgtttt ttctgaatta acagctgctc ctacaaaaga tggcattcgg
    11881 tttgaattaa agggcttaac agagacgagg gaagccgatt attacccagg ttttcaagca
    11941 aagtttattg cttttcttga aaatgcacgt gtacctttta aattagatat tacttcaggt
    12001 gattctatta ttccagcagc attgatatat gaacataaat tgatgtttga agataaaaca
    12061 attcatattc cagcttatcc tacggaacaa attttagctg aaaaattaca tgccacgttt
    12121 agtttcggaa tagataattc acgtatgaaa gatttctatg atctgtatat gatccctaaa
    12181 ctagaacgta tcgatgagat aaatttgtat aagagtgttc aaggaacatt taaggaaaga
    12241 aataatcaat cagtgtttaa gaatttgtat aatcaggaaa tgcctcaatt gatgaagaat
    12301 gagggattga gggagaaatg gaaaagatat cagcaagaaa actattttgc caaagatatt
    12361 tctttcgaat ctaccttaac ttctgttgat tcgctgatgc aatcaattgt cgagcaagag
    12421 gaagtagaac gaaaacaaaa ttttaagaga tcacaacaaa tgaatcaaga aatggagaga
    12481 taaagatgat caataatgta actttagtag gtcgattaac caaagatccc gatttacgtt
    12541 acaccgcaag tggcactgca gtgaccactt ttaccttagc cgttaaccgt aattttacta
    12601 atcaaagtgg caatcgagaa gctgacttta tcaattgtgt gatttggcgc aaaccagctg
    12661 aaacgatagc gacattagct aaaaaaggca gcttgatcgg cgtcgtcgga cggattcaaa
    12721 cgcggactta tgacaaccag caaggacagc gtgtctatgt gactgaagtt gtggccgata
    12781 atttccaact attagaaagt aaagcagcga cagaaagccg aacgcatact gatcaatcgt
    12841 ccaccagcac cagtccaagc accaccgctt ttgaacaaca tgaaaaacta ccaactaaca
    12901 acaatggatt taatgattca cagaatccat ttggtggaca atcaatcgat attagtgatg
    12961 atgatttacc attctgattg taaaagatca ataaagttgt tataatggat acgaaaagag
    13021 gagttgcgct aacaaccccc ctaacagcac cgtttaagac ggtgaccagc ttaatattat
    13081 ttttgaaaaa taatcgtcga ccggctaaag ctgacggtta ttttttttga ttgttgttta
    13141 caactgctaa gattccaaaa atgattgttg cgatcaaaga accgaaacta atcattaatc
    13201 ctagcgccgt ttcaacaggc aaaaggcttc tcctttctgt aagattttgg aagtacgcac
    13261 atacgcacca cctcactttc aatcggatag ccaccgtcac aaactttact gtcttgatca
    13321 ttatagcata tctaaaatcg aataaaaatt ctcaatttta gctgtttttg ctataatttt
    13381 agcgaggtga tcgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa
    13441 acacaaccat tattggttcg ctttacttta caaacaattc atgaatcgat taattgtgta
    13501 gttgcagata ttgaaattat cgataaactg ctaattatgg atgatggaaa atacaatatt
    13561 gccgtcacag gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga
    13621 aatcccgatc attttacgcg atcgatgggc atataaataa aagaagagtt gtagtcctaa
    13681 tgtagga
//
LOCUS       DAJSNV010000068        13242 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSNV010000068 DAJSNV010000000
VERSION     DAJSNV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.071"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..310
                     /locus_tag="ON516_001968"
     CDS             11..310
                     /locus_tag="ON516_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345444.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(407..841)
                     /locus_tag="ON516_001969"
     CDS             complement(407..841)
                     /locus_tag="ON516_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345445.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1073..1783)
                     /locus_tag="ON516_001970"
     CDS             complement(1073..1783)
                     /locus_tag="ON516_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3345446.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1773..2633)
                     /locus_tag="ON516_001971"
     CDS             complement(1773..2633)
                     /locus_tag="ON516_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3345447.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2638..3276)
                     /locus_tag="ON516_001972"
     CDS             complement(2638..3276)
                     /locus_tag="ON516_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3345448.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3291..3590)
                     /locus_tag="ON516_001973"
     CDS             complement(3291..3590)
                     /locus_tag="ON516_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345449.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3633..5117)
                     /locus_tag="ON516_001974"
     CDS             complement(3633..5117)
                     /locus_tag="ON516_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3345450.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5137..5598)
                     /locus_tag="ON516_001975"
     CDS             complement(5137..5598)
                     /locus_tag="ON516_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3345451.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5616..6683)
                     /gene="ulaG"
                     /locus_tag="ON516_001976"
     CDS             complement(5616..6683)
                     /gene="ulaG"
                     /locus_tag="ON516_001976"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3345452.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6901..7671
                     /locus_tag="ON516_001977"
     CDS             6901..7671
                     /locus_tag="ON516_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3345453.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7944..8771)
                     /locus_tag="ON516_001978"
     CDS             complement(7944..8771)
                     /locus_tag="ON516_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345454.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9339..10574
                     /locus_tag="ON516_001979"
     CDS             9339..10574
                     /locus_tag="ON516_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3345455.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11573..11833)
                     /locus_tag="ON516_001980"
     CDS             complement(11573..11833)
                     /locus_tag="ON516_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3345456.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11860..13161)
                     /locus_tag="ON516_001981"
     CDS             complement(11860..13161)
                     /locus_tag="ON516_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345457.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4203 a         2759 c         2082 g         4198 t
ORIGIN      
        1 gaaataaaac atggattata ctgttgaatt tgacagtgaa ctacttaagc aatttccaca
       61 agaagaacat ttcatgtata aagaagcaat ttcccacaaa gtgacagagt atttggagca
      121 taattttttt cgtataaagc cagtgcgtac tcgttcggca aaaggaatat acgagatgaa
      181 agtcaaaatt aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa atcatgtgtt
      241 cattttttat attagttcga atttgcaaaa aattgtattt gataaagagg tgagtaaaaa
      301 aataaaatag ctgagcattg gaggatagat tgagtgatac taagtctatc ctcttgtttt
      361 tctaaagaac taagctagaa ttttttcttt tgtacgttat tatatgttat ttgtcattac
      421 caaaaagcac cttttttctt ctagtatcta tgacataaga aataccaccc atttccttta
      481 cttgaacaaa tcctactgat tcataaaatt tttttgtttt ctcagtatca tctgtcaata
      541 attcgatttg gactggtcca taactagtga tcttctccaa agcttcattc aaaagcactt
      601 tgcctattcc ttggcgctga tacctcggat gaatgaggat atcttgaata aatgcgatcg
      661 tcgcaaaatc agtgatccaa cgaacgattc cgataatctt cccttcccca ttacgcttta
      721 tcagagattc agattgttca aatgctctag ctaaccttgc agtgtctttt gtgtaattcg
      781 tccaaccaac gctcttatat aattcagaaa tctcatcgat cataagggaa tcaatattca
      841 tttttttctt cctttcttat acaattctcc ctctaataat acagttcttt gactatttaa
      901 tctatcacat ataactctcc tgtaaagatt ctctcgattc cttaaaattc ctctttcctt
      961 ttagaatgca tactttttga taaataaaaa ggttgtgata aattttcttc acaacctctg
     1021 tttctctcta tcaagtgtta tcttttaatt ttctaccctt accctagcat ctctattgcc
     1081 cgtagtatgc atactttcca tgtttgcgaa aataatgttt atctagtaaa tattgcggga
     1141 tttcacatat attggggttg atcagctcgt tttctttggc catcagacaa atttcatcta
     1201 agatcaaact gttttctact gctttcatag gtgaatcccc ccatgtgaac ggtccatgtc
     1261 catagacaag tactccaggg acttcgtttg gatctaattt tctttcctta aacgtctcga
     1321 caatcacatt ccctgtatga acttcgtaag cttctcttac ttcttctttc gttaattggc
     1381 gtgtacaagg aaccttacca taaaaagcat cagcatgagt cgtgccataa gctggcaaat
     1441 cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg agtatgagta atagcagtta
     1501 tatcttcaaa agtttgatac aaaacaacat gtgtggggag atctgaagat ggttttaagc
     1561 tatcttcttc tattatattt cccgacaaat ctgttaccac catttgatct gcttgcatgc
     1621 attcatatct gacaccgctt ggcttaatga cgatgactcc tagctcacgg tccacttcac
     1681 tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga tttgcttcaa
     1741 ggacacgttg cttcattgct gcaatttttt cttcacaaga catcatagcc agcctccttc
     1801 aatttagaac ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc ttggcttttt
     1861 tcactccaca tttcaatcag aaatgttcca tcataatcta gacgtttcaa atgtcttaat
     1921 aatccaagaa aatcaacaca accgctacca aaaggaacat cacgaaactg gcctgaatat
     1981 tcttttgtta cagggtaagt atcttttaag tggatagcag tgatataatc gatccctttc
     2041 tcaagttctc gagccggatt attttccggc catgcggaca aattgcctag gtctggatat
     2101 acttgtagaa aaggtgaatg aatctgttct ttgatttcta aaaattttga aatggagttc
     2161 ataaatggat catccatgat ttccacggaa agaatgatgc catatttaga agcttccttt
     2221 acgctttctt ttattccttc gataaagtct tctctggaat tcattgattt ctcttcataa
     2281 taaacatcgt atccagctat ttggattact ttgacgctta gattatgcgc caaatggatt
     2341 gcttttttca ttagttgttt cgctgctttt ttcttttctg gatcagcaga accaaacgga
     2401 aatcgccgat gcgcacttaa acaaatcgaa tggatacgta caccggagct gaacatctct
     2461 tctctcagct cagctatctt ttcttcagac caatctaatc gtgctaaccg ctcatccgtc
     2521 tcatcaatcg acatctcgat aaaatcgaag tccatttctt ttacaaggga aaaacgttct
     2581 ttccatgaaa tatctttggg taacgctttt tcataaatcc caatttgagc catttcctta
     2641 cccccagata cgaatgatct catctttgaa gttttgtgcg gccttcatcg gatcatttgc
     2701 tgctgtaata ccacgaccag tgataaaggt atacacatcg atcccttcaa ataagtgtaa
     2761 tgtgtgtgga tttaatccac cagttacaga aacattaaag cctagttcaa tcagtttttt
     2821 gatcttcgat aaatcttttt caccccaagt ttcaccagat aacagcgcat cacggctttg
     2881 atggtagatt acctgccgga taccgatgtt atgccaatcc atggcttgtt caaaagtcca
     2941 attgccgtag agttctactt gcaattcacc tatttctttt tgtgctgcct ccatagttgg
     3001 aatcgttgca caacaaatga ctgtcatcca gtctgctcct gcttttgcaa cattactcgc
     3061 cactgttcct ccagcgtctg cacatttcgt atcagcgact agttttttat ctgggaacat
     3121 actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg tgcccacttc
     3181 aataatatct actacatcac caactttcat aacatctcct aatgcatgtt ctaagtcatt
     3241 gtgatctaac gcaatttgca aattcggcct gctcatctta gttccttcct ttacttgttt
     3301 ttttggtacc actttgtttc agcaaatttt tcactgattt ctttgactga catcacattt
     3361 ttgataccga taactgtaac tccttttttc tctgcctgag taaacatatt caaaaaattg
     3421 actggcgtaa aaactacatc gtattggcca gctgaacttt ttccctctga aatggcacaa
     3481 tgatggattt tggtaattgg tacgtttagt tctttcattg ctttttccac acttctcatc
     3541 atcatcaagc ttgttcctga tccatttgca cacgatacta aaattctcat cttaatccct
     3601 ccattatgag tgttgtctaa tcagattttc ttttatactc tctgcttttc tgcatatgtg
     3661 tcatagtctt ctacaatcaa aaaataacca tcaggatttt tccgatattg taactgcgga
     3721 atagccaata aaccaatgac aactacggca attccagcat aacctaggac tttcatgatt
     3781 actgtaaaca atggccaagc tgttgcccag tcgaacatcc cgatataacc accgtatgtt
     3841 gacatcccaa tccatgtagc gaataatgct gaaccgccta cttgtatgat tcctgaaaga
     3901 aacgggaaca ggcaagcggc tttgaatcca cctcggttat tggcataaac tgctataacg
     3961 gcattatcaa agaacaacgg aatgaaaccg gctatgacga tcgttggtga tttcaataaa
     4021 atcagtaaac caatcatcaa aaactgtccc aaagcaccaa acaagaaccc aatcgttacc
     4081 gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac agctcctggt
     4141 aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt catcgacgaa tgtccgaaca
     4201 cctaattgca agatcgccag atataccgca aaatttaaag aagtcgtcat aatgtagaat
     4261 aagaaactct gcccttcttg catgaattgc gcttgaatca aataatcttt gcctagtaca
     4321 atcagaatga tcccaaagaa aaatagcatg agtaacgaag tagcaaccat attctcattg
     4381 aaaatcgaca agaatcctgg taatttgata tcttcaattt ttttgtcttg cttgattgat
     4441 ctatttttct tttcatcccg ctttttcatc cactcagcta attttgcaaa tataaagatt
     4501 ccgaacattt gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc ctgcgtgatt
     4561 cctactgtta agttagaagc tactgtccaa taacaaccta aaatcagtcc cataaaaatc
     4621 aatgcttgta tcctacctag atcaggaaag caaaacagta aaatccaaaa agcggtagct
     4681 gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt atatttctga
     4741 aaacggacta ataagatatt taaaacaaat gcaatcagta ataaaatcat cacatctcca
     4801 aaggtacggc caaatgtctc cattaatcca gcatccacag cattttgtcc aaaataagga
     4861 tctgtaacca tggcatccaa attgaaccgt tcctttagtc caaccaagat tggccgaaaa
     4921 ttattgacca aaccaccaga gccaacagtc aaaataaagt aaccgaccgt tgcctttaag
     4981 aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa taacacgata
     5041 aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt ccaaatgctt
     5101 aacaaaaatt cccccatctt ttttctcctc tatcaattaa ttatattctg ccatcacaac
     5161 ttggtaatca tcgatggagt tcgtagagat gagacgctca accaatccat ctgtcattaa
     5221 taattcggat aatttttgga tattttctag atgttcttct ggatcttttg ctgcgagtgt
     5281 aaaaaacaaa ctagccgttt tctcctcatt ctccccttca ctttcaaaac ggattggctg
     5341 tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca tcactttttt ctgaagaatg
     5401 cggcattgct acttgaggtg cgatcacaat ataaggaccg tatttttcta cacaatggat
     5461 gatctcctcc acatacgttt catcaataat ttccttatcg atcaatacaa gacagctttc
     5521 ggcgatagct tcacgccaat ccgtgatttc tttatctggg taatgaatca attgatggtc
     5581 aaaaaagtat ttcagcaaga aactcccctt ctttcttata aaaacgacgg aaacggtgta
     5641 tcattttcaa tcgagaagac atcttcaaat cctcgataaa aattaaactc aagatcatct
     5701 ttgtttgtcg gatagatgaa tttcccgcct acttgccaga tataaggttt aaatccatac
     5761 ttcaggcgat cctttttgaa tttccaaatt tcagtgatct cttttggatc agccataaaa
     5821 ttagaccaaa tatcgtagtg gacaggaatc actacttttg cattcaatga ctccgccatg
     5881 cgcagcatat caacagaagt aactttgtct gttatccctc gtgggttttc accataggcc
     5941 cctaaacaga catcgatttt gtgttcatta ccatgtttgg caaataaatt ggagtaatgt
     6001 gaatctcctg catgataaag attccctcct gatgtttcga ataaatagtt tactgcaatt
     6061 tgatccatct cctgaggcat ttttcctttt aatacctctt ctggatcttc acaagtgacc
     6121 aacgctgtcc gatcaaacgc ttctaaagct actacattga tgtctttgat tttgactgca
     6181 tcacctggct ttactgtaat cgttttttct tcaggaattc cccatttttg ccacgtgcga
     6241 accacttctt ttggcccaat gaatgttgct tcaggacaat tttgatgaac tgctgcggct
     6301 gtatggatat ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc tacatttttt
     6361 atcgcaaaag gatcgattac gaaaggctga gtacgtaaat taggctgcat attttgaacg
     6421 ccactcatac gcatcatctg atggcctttt ttcatcatgc cattcccatg actacgtttt
     6481 cccgtcccac accatagatc acataaaata ttcgttcctt catgactttt cagccaaatt
     6541 cccgtacagc ccagccacca catagcaaaa gttccaggat ccactacttc tgtttcgatt
     6601 tcttcattca agtatgtgcc ccactcagga aatgtttcta ctaaccactt ttcttttgtt
     6661 acttcattga cagttttact catttgcttg ctccccccat atcgttgttc caacttacaa
     6721 gtaaatatta atgtgtatca gcatccatga aaaccctttt atatgaccgt ttgtttttta
     6781 tatgattttc tttcagtaat caatgattgt ttcagtataa taaagacaga tatgaatgtt
     6841 taaatgaaca actagtcaaa taaatccatg gatcaagatg tccctcagga ggcaaaagtc
     6901 atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga actgcttcag
     6961 cagactgaac aactatcaac tttcgaatta gctgaatccc ttgatgtatc tattagtacg
     7021 atacgaagag atttaaattt attagaagaa aaaaacgaga tcatacgcaa ttacggttat
     7081 tgttcttata attacaaaaa ccagactgat tttgatcaat ctggtcctga aaggattaaa
     7141 caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct gtttatcaat
     7201 tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt gacagtcatt
     7261 acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta tattttaact
     7321 ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt aaatacgatt
     7381 tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt agtacatggg
     7441 gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa caagacaatc
     7501 aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa gttcaagatt
     7561 gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga aaaagtatta
     7621 gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta agaaccagga
     7681 cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga taaatgaaaa
     7741 acacgctacg ctcaaattga taaaggaatc ccaagatgta tccatccaat cgataccatc
     7801 ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc aaacaatatc
     7861 gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg tattcaaatc
     7921 agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt taccgcgtaa
     7981 tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa ataatcatct
     8041 tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa tgcataagtc
     8101 gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc tttatcataa
     8161 tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac catcagatta
     8221 tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt cgcaaaatct
     8281 aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa gtgatggata
     8341 tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc atctggtttt
     8401 ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa aagatcagaa
     8461 ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg gtattttgat
     8521 acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc ttctgtttct
     8581 actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa gatattgatg
     8641 cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca aatgccttct
     8701 gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa tgctccatct
     8761 actttaatca aaacaatttc cttcccttta aaaaattatt tctcctttat atcatacaca
     8821 aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat aaaaatttta attttaaaat
     8881 aatattcaaa ttaaacaact ggcgtatctg ctccttgatt tctttttgtg ttatctgaat
     8941 aattatcgtt tacagttata acacatagtg aaaattgtca tggcgagaaa gtctaagaat
     9001 atttttaaac ataactattt ctttcttacg tgtgtataat gacgacagca cacctatcaa
     9061 ttaatataat tattttattt ttttatgttc tcatgtttat ttttaatgac acaacttctc
     9121 gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt ttttgaaaat
     9181 tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag cgagagacag
     9241 tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta tactagtaag
     9301 aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt ctttttgctc
     9361 ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa gctatgaaag
     9421 accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg atcacagaaa
     9481 gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa gaacaaatca
     9541 taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg gttgcctctc
     9601 gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt gtgacaggtt
     9661 cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta tatgttaaac
     9721 cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc cacgagttac
     9781 gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt atagtaaata
     9841 cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt cctttaccaa
     9901 aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct aaactatttt
     9961 tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt gctaagctca
    10021 gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa gaaagaaaac
    10081 gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc aaaattttgt
    10141 tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat ctgcttgatg
    10201 acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt catcttttgc
    10261 atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa actgcctgtg
    10321 aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt aatattgact
    10381 attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa agaaaactgc
    10441 agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc acgctgcaag
    10501 ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc ttcctgagca
    10561 accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat cagtaatttc
    10621 gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt gaaaagttag
    10681 agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa aagatctgac
    10741 gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt ctttgacacc
    10801 tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata attaggcaaa
    10861 tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga gatttgtgcc
    10921 gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat ctttgataat
    10981 caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc ctatataaac
    11041 tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc gtcagcaacg
    11101 atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat tatttctata
    11161 cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat acaccatact
    11221 gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat cactttggta
    11281 gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc tccttctttc
    11341 tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga caaaagcctt
    11401 gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg gtaataagtt
    11461 caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt ttcgtaaatt
    11521 tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac atttaaagct
    11581 tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa attccacaaa
    11641 gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc tttggaagaa
    11701 tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata tgatatgtag
    11761 ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca aaaaataaaa
    11821 tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct ggacttcgtt
    11881 ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta caaatggata
    11941 atagataata aatgctatca atgtgttgat catgatcaag ataacaacac gccagtcatt
    12001 tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca aaacagtaaa
    12061 tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa ctaaaggaac
    12121 taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa aaattatggg
    12181 ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt tcaagaattt
    12241 tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg caccaccaat
    12301 tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg tggcagctgt
    12361 accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg gacgaacaac
    12421 agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga tcaaaaacat
    12481 tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct cgaataacga
    12541 taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag taattgttaa
    12601 aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg gcacttgttc
    12661 tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca ctgcaaaaat
    12721 catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag gtaagaccca
    12781 gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca taaagaacgt
    12841 tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag ctagatccga
    12901 acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa agggtaaaac
    12961 aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta aattgataaa
    13021 gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc cattacgaat
    13081 tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt tctcttcgac
    13141 ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga accattcatt
    13201 gcatgacaac gcaaagaatg ttcataacta attatataat ta
//
LOCUS       DAJSNV010000069        13183 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSNV010000069 DAJSNV010000000
VERSION     DAJSNV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.072"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..186)
                     /locus_tag="ON516_001982"
     CDS             complement(<1..186)
                     /locus_tag="ON516_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345458.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGL"
     gene            complement(202..816)
                     /locus_tag="ON516_001983"
     CDS             complement(202..816)
                     /locus_tag="ON516_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3345459.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(963..1646)
                     /locus_tag="ON516_001984"
     CDS             complement(963..1646)
                     /locus_tag="ON516_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3345460.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1755..2213)
                     /locus_tag="ON516_001985"
     CDS             complement(1755..2213)
                     /locus_tag="ON516_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3345461.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2297..4060)
                     /locus_tag="ON516_001986"
     CDS             complement(2297..4060)
                     /locus_tag="ON516_001986"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3345462.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4281..5741)
                     /locus_tag="ON516_001987"
     CDS             complement(4281..5741)
                     /locus_tag="ON516_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345463.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5949..6875)
                     /gene="glsA"
                     /locus_tag="ON516_001988"
     CDS             complement(5949..6875)
                     /gene="glsA"
                     /locus_tag="ON516_001988"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3345464.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6898..8325)
                     /locus_tag="ON516_001989"
     CDS             complement(6898..8325)
                     /locus_tag="ON516_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3345465.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8356..9570)
                     /locus_tag="ON516_001990"
     CDS             complement(8356..9570)
                     /locus_tag="ON516_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3345466.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            9920..10999
                     /locus_tag="ON516_001991"
     CDS             9920..10999
                     /locus_tag="ON516_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3345467.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(11300..11542)
                     /locus_tag="ON516_001992"
     CDS             complement(11300..11542)
                     /locus_tag="ON516_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3345468.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11574..12131)
                     /locus_tag="ON516_001993"
     CDS             complement(11574..12131)
                     /locus_tag="ON516_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3345469.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12144..12332)
                     /locus_tag="ON516_001994"
     CDS             complement(12144..12332)
                     /locus_tag="ON516_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3345470.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12345..12911)
                     /gene="amaP"
                     /locus_tag="ON516_001995"
     CDS             complement(12345..12911)
                     /gene="amaP"
                     /locus_tag="ON516_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3345471.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3838 a         2809 c         2310 g         4226 t
ORIGIN      
        1 caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag taacagtagt
       61 aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca gaaaatcatt
      121 cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat agcttatttt
      181 taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt ctcctattgc
      241 tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat cgatccttcc
      301 tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta gcaaaaataa
      361 atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg atgtttcaaa
      421 acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag ttaaagtcaa
      481 gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt tatttacttc
      541 ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa gaatattctt
      601 tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga tgggtcgatt
      661 cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt ttgctgattt
      721 agtcgttact actgctactg gtctagggat gactgaacca atcagcaact tgtaattttc
      781 acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt tctataaaat
      841 tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca tcttcttaga
      901 atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc cgtgcgtgtt
      961 ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa ttgcggacgg
     1021 gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt ctctacttgt
     1081 tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc tatgatttga
     1141 ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata gcctgctaaa
     1201 gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc aaaagtcgaa
     1261 atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc agcagttgtt
     1321 ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc ttgcgtctcc
     1381 aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc atcgtaatct
     1441 cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt gatatcttct
     1501 gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc cattctcccc
     1561 ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac gacgactccg
     1621 cctttgattt gttccagtgt atccacaata tctcctccag taatgttata taaaaataca
     1681 gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct atttctttca
     1741 gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac cagcttctct
     1801 tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc agcatggact
     1861 ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg ctctttcgtg
     1921 acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac tggttcattt
     1981 tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt ctcttgttta
     2041 tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag ttcgctcaac
     2101 tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc tgattgataa
     2161 ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata cataacatcc
     2221 acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca attatctgga
     2281 tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca acgatttgtt
     2341 ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc gtgaattctt
     2401 tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga ctgatatttt
     2461 cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg tgtgtttctt
     2521 ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg atcaacgttg
     2581 cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca agaaaaccgc
     2641 ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct ggaatactat
     2701 aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg attgctacag
     2761 gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct tttgttgctg
     2821 gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat gttccttggt
     2881 gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact ggattctcgt
     2941 tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta cttgattgga
     3001 agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata gagtgctgtt
     3061 cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt ttcagtccaa
     3121 aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt tgcattttct
     3181 tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact tgctttgcct
     3241 ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta tcgattgccg
     3301 gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga tcaatatctt
     3361 ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc acgtaacgat
     3421 aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt tgacgaagct
     3481 cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca ttatcgttta
     3541 ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca aaagctaagc
     3601 caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct ttcttcagat
     3661 ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc cccactgtaa
     3721 aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg taatgtcctt
     3781 tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga tgggaaacat
     3841 cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc gttaattcta
     3901 ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc aagatttttt
     3961 ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg acatctgcgg
     4021 ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct ctcctccgta
     4081 ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta cttagagttg
     4141 actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat aagtagatct
     4201 tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc tttgtagaag
     4261 gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag ttcctgagca
     4321 atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt tgtcttttca
     4381 gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt tttcgcacat
     4441 agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat ttcagcgatg
     4501 ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac tgaactttct
     4561 gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat caaagaaaga
     4621 cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg atttttcttt
     4681 ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt ttctgataga
     4741 agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg gacaaattgt
     4801 aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat actaataatc
     4861 tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata aacaacttcg
     4921 aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt gaacaaagaa
     4981 aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc ttccaatcct
     5041 tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc ttctttcagt
     5101 ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat aaaggacttt
     5161 tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat caactcgtta
     5221 caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac ataacgtact
     5281 aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt atttttcaaa
     5341 taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac tttgctcaaa
     5401 gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa ataataactt
     5461 aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga aagagcaata
     5521 ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt tatacaatat
     5581 tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt cacccttaga
     5641 gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc acacagatac
     5701 acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac acgcctccag
     5761 tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc ctttttattt
     5821 agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca aacaagctca
     5881 caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc agcctctttc
     5941 aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt caacaattgg
     6001 ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc tatgccgtaa
     6061 cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg tacaccaaca
     6121 tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat tgatttgacg
     6181 attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat tcctttattt
     6241 gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga gcattgtctg
     6301 aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc tttcatatag
     6361 taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat ttcttcattc
     6421 aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg ctcaaatttt
     6481 tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa
     6541 ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt gaaggcaaaa
     6601 ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc tttgattgct
     6661 aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct
     6721 ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg tttgggatcc
     6781 acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata gtggaaattt
     6841 tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt tcatttttta
     6901 tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa taactaagac
     6961 gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag ctgcgatact
     7021 agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc
     7081 aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa gactgtcatc
     7141 tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca ggtagattac
     7201 aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc ttaatgtaaa
     7261 gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag caggaacacc
     7321 gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg ctacgtgaag
     7381 tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta aacaaacaag
     7441 aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta ataggaattc
     7501 taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg tagtagcgac
     7561 ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa acattgctaa
     7621 tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg tttctacgcc
     7681 catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg aaaaatcagg
     7741 aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga cataatggat
     7801 ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac cgatccgagc
     7861 aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc agaaaataat
     7921 caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac ccgttgcata
     7981 ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta tctcaaacca
     8041 ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga cccaagtata
     8101 gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca atgccacagg
     8161 gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc ctgaggtcgc
     8221 gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca aaaagccaaa
     8281 gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc ttcctctttt
     8341 ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca atccttcttc
     8401 ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta aaatcactgt
     8461 tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa tccctaagaa
     8521 gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc ctatcagaat
     8581 cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat catcccattg
     8641 tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga tcccaatcag
     8701 catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca atccagcaac
     8761 ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg caatgatcag
     8821 ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat tgactgcttg
     8881 ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc aaagcagtcc
     8941 tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta aaggaatctt
     9001 tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg tgtggatgac
     9061 tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac cccatatcca
     9121 gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa tatagccttt
     9181 taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca cgataaacat
     9241 cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca tgttggggaa
     9301 gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac cgccgatatc
     9361 ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta tcccaacgga
     9421 gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc cgccgtaaaa
     9481 aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca tgaatgctgt
     9541 atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac gattacgagt
     9601 ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact tgcatttata
     9661 aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt tcacacaaat
     9721 aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg tcttttttta
     9781 tttttaaaga tattttttca taaggaataa agaggcggat tttataatta acttagccat
     9841 ttggaaataa aaaaacgcca cgtgaattcc atgttatatt gaagttacca caactctcaa
     9901 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
     9961 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
    10021 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
    10081 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
    10141 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
    10201 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
    10261 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
    10321 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
    10381 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
    10441 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
    10501 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
    10561 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
    10621 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
    10681 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
    10741 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
    10801 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
    10861 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
    10921 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
    10981 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
    11041 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
    11101 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat
    11161 gggtggctaa cttaaacttg aaatttacgt aaggaataaa gaatacttca tataaaatat
    11221 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
    11281 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
    11341 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    11401 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    11461 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    11521 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    11581 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    11641 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    11701 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    11761 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    11821 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    11881 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    11941 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    12001 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    12061 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    12121 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    12181 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    12241 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    12301 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    12361 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    12421 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    12481 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    12541 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    12601 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    12661 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    12721 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    12781 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    12841 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    12901 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    12961 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    13021 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    13081 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    13141 tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DAJSNV010000070        13111 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSNV010000070 DAJSNV010000000
VERSION     DAJSNV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.073"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            628..1161
                     /locus_tag="ON516_001996"
     CDS             628..1161
                     /locus_tag="ON516_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3345472.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1651..2175)
                     /locus_tag="ON516_001997"
     CDS             complement(1651..2175)
                     /locus_tag="ON516_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345473.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="ON516_001998"
     CDS             complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="ON516_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3345474.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2764..3744)
                     /gene="pta"
                     /locus_tag="ON516_001999"
     CDS             complement(2764..3744)
                     /gene="pta"
                     /locus_tag="ON516_001999"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3345475.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3897..4577)
                     /locus_tag="ON516_002000"
     CDS             complement(3897..4577)
                     /locus_tag="ON516_002000"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3345476.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4703..5572
                     /locus_tag="ON516_002001"
     CDS             4703..5572
                     /locus_tag="ON516_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3345477.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5598..6113
                     /locus_tag="ON516_002002"
     CDS             5598..6113
                     /locus_tag="ON516_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345478.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6160..6753)
                     /locus_tag="ON516_002003"
     CDS             complement(6160..6753)
                     /locus_tag="ON516_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345479.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6778..6957)
                     /locus_tag="ON516_002004"
     CDS             complement(6778..6957)
                     /locus_tag="ON516_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345480.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(7034..7543)
                     /locus_tag="ON516_002005"
     CDS             complement(7034..7543)
                     /locus_tag="ON516_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345481.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7639..8019)
                     /locus_tag="ON516_002006"
     CDS             complement(7639..8019)
                     /locus_tag="ON516_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3345482.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8349..9098
                     /locus_tag="ON516_002007"
     CDS             8349..9098
                     /locus_tag="ON516_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3345483.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9282..10559)
                     /locus_tag="ON516_002008"
     CDS             complement(9282..10559)
                     /locus_tag="ON516_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345484.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="ON516_002009"
     CDS             complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="ON516_002009"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3345485.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11481..12425)
                     /locus_tag="ON516_002010"
     CDS             complement(11481..12425)
                     /locus_tag="ON516_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3345486.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12427..13083)
                     /locus_tag="ON516_002011"
     CDS             complement(12427..13083)
                     /locus_tag="ON516_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3345487.1"
                     /translation="MIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFV
                     NNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNV
                     SFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGG
                     AGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ
                     "
BASE COUNT         3786 a         2737 c         2336 g         4252 t
ORIGIN      
        1 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
       61 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
      181 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
      241 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
      301 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
      361 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
      421 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
      481 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
      541 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
      601 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
      661 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
      721 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
      781 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
      841 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
      901 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
      961 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     1021 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     1081 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     1141 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     1201 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     1261 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     1321 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     1381 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     1441 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     1501 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     1561 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     1621 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     1681 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     1741 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     1801 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     1861 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     1921 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     1981 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     2041 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     2101 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     2161 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     2221 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     2281 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     2341 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     2401 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     2461 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     2521 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     2581 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     2641 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     2701 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     2761 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     2821 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     2881 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     2941 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     3001 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     3061 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     3121 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     3181 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     3241 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     3301 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     3361 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     3421 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     3481 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     3541 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     3601 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     3661 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     3721 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     3781 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     3841 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     3901 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     3961 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     4021 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     4081 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     4141 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     4201 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     4261 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     4321 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     4381 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     4441 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     4501 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     4561 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     4621 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     4681 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     4741 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     4801 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     4861 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     4921 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     4981 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     5041 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     5101 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     5161 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     5221 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     5281 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     5341 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     5401 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     5461 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     5521 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     5581 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     5641 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     5701 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     5761 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     5821 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     5881 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     5941 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     6001 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     6061 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     6121 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     6181 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     6241 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     6301 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     6361 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     6421 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     6481 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     6541 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     6601 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     6661 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     6721 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     6781 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
     6841 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     6901 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     6961 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     7021 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     7081 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     7141 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     7201 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     7261 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     7321 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     7381 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     7441 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     7501 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     7561 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     7621 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     7681 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
     7741 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     7801 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     7861 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     7921 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     7981 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
     8041 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     8101 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
     8161 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
     8221 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
     8281 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
     8341 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
     8401 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
     8461 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
     8521 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
     8581 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
     8641 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
     8701 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
     8761 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
     8821 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
     8881 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
     8941 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
     9001 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
     9061 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
     9121 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
     9181 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     9241 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
     9301 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
     9361 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
     9421 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
     9481 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
     9541 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
     9601 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
     9661 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
     9721 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
     9781 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
     9841 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
     9901 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
     9961 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    10021 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    10081 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    10141 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    10201 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    10261 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    10321 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    10381 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    10441 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    10501 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    10561 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    10621 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    10681 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    10741 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    10801 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    10861 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    10921 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    10981 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    11041 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    11101 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    11161 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    11221 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    11281 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    11341 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    11401 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    11461 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    11521 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    11581 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    11641 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    11701 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    11761 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    11821 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    11881 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    11941 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    12001 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    12061 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    12121 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    12181 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    12241 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    12301 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    12361 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    12421 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    12481 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    12541 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    12601 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    12661 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    12721 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    12781 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    12841 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    12901 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    12961 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    13021 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    13081 caagacgcct aaaaggctaa gataattttc c
//
LOCUS       DAJSNV010000071        13056 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSNV010000071 DAJSNV010000000
VERSION     DAJSNV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.074"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            157..1080
                     /locus_tag="ON516_002012"
     CDS             157..1080
                     /locus_tag="ON516_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3345488.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1086..2339
                     /locus_tag="ON516_002013"
     CDS             1086..2339
                     /locus_tag="ON516_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3345489.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2473..3819
                     /gene="gor"
                     /locus_tag="ON516_002014"
     CDS             2473..3819
                     /gene="gor"
                     /locus_tag="ON516_002014"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3345490.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4633..5586)
                     /gene="menA"
                     /locus_tag="ON516_002015"
     CDS             complement(4633..5586)
                     /gene="menA"
                     /locus_tag="ON516_002015"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3345491.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5599..6702)
                     /locus_tag="ON516_002016"
     CDS             complement(5599..6702)
                     /locus_tag="ON516_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3345492.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6792..7748)
                     /locus_tag="ON516_002017"
     CDS             complement(6792..7748)
                     /locus_tag="ON516_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3345493.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8029..9963
                     /locus_tag="ON516_002018"
     CDS             8029..9963
                     /locus_tag="ON516_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3345494.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10420..10836
                     /locus_tag="ON516_002019"
     CDS             10420..10836
                     /locus_tag="ON516_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3345495.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10850..11416
                     /locus_tag="ON516_002020"
     CDS             10850..11416
                     /locus_tag="ON516_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3345496.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11439..12425
                     /locus_tag="ON516_002021"
     CDS             11439..12425
                     /locus_tag="ON516_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3345497.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="ON516_002022"
     CDS             complement(12580..>13056)
                     /gene="scm"
                     /locus_tag="ON516_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3345498.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4064 a         2369 c         2697 g         3926 t
ORIGIN      
        1 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
       61 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
      121 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
      181 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
      241 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
      301 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
      361 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
      421 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
      481 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
      541 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
      601 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
      661 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
      721 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
      781 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
      841 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
      901 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
      961 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     1021 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     1081 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     1141 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     1201 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     1261 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     1321 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     1381 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     1441 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     1501 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     1561 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     1621 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     1681 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     1741 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     1801 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     1861 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     1921 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     1981 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     2041 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     2101 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     2161 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     2221 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     2281 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     2341 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     2401 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     2461 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     2521 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     2581 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     2641 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     2701 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     2761 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     2821 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     2881 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     2941 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     3001 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     3061 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     3121 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     3181 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     3241 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     3301 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     3361 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     3421 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     3481 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     3541 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     3601 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     3661 gaagaaagaa tcgtcggctt acatgctatt ggtgttggtg tcgatgagat gctgcaaggt
     3721 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     3781 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     3841 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     3901 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     3961 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     4021 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     4081 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     4141 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     4201 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     4261 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     4321 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt tacaacctct
     4381 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
     4441 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     4501 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
     4561 taaggagcta tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac
     4621 ctcttcatgc tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact
     4681 ggcattgaat aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt
     4741 ttgcttgaat tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat
     4801 cggccattca tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat
     4861 caatagctgg aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg
     4921 atgattttca atatcacggt caagatcccg caaattatta gccaacatga tattggcaat
     4981 cgtaaacacc aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac
     5041 caaagcaaat tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt
     5101 attgacataa acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc
     5161 ccctaaaggc attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca
     5221 acagataaat cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat
     5281 aacaaaaaca aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt
     5341 tctgcggcca atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc
     5401 catgtaatta ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa
     5461 aaaagtgttt agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc
     5521 aaaaggaaat acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag
     5581 tgtcatggtt cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc
     5641 ctccgataat ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt
     5701 cacgaagatc gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt
     5761 cacacccatt gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat
     5821 gatcgtgaca cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg
     5881 atggtatttt ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt
     5941 tttgttccgt tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat
     6001 gccgactgtc cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc
     6061 gcccaagtca acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc
     6121 cgtaatgtaa cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata
     6181 aacagttttt tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg
     6241 atcgatttct tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt
     6301 gattgcacca atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg
     6361 cttgactaac gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt
     6421 cacttcatcg atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac
     6481 tcgatcgaag gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac
     6541 gtaagtccct aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc
     6601 tgaactttca gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga
     6661 gatgcccagt gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt
     6721 ttgaaaggtg agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta
     6781 tgattatgtg attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct
     6841 ttttcagctg cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta
     6901 gcacctgtaa caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg
     6961 aattcgtcgt tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata
     7021 ttttcattat attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag
     7081 tttgattcag ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta
     7141 gagtagttct tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt
     7201 tcgattgtat ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg
     7261 aagctttcag atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct
     7321 tttaagaatt ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct
     7381 tccatgtttt tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta
     7441 tcatattttg attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa
     7501 ccatttgaat aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct
     7561 acaacttttg cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat
     7621 tcagaagaag agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct
     7681 gaaaatgcta atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac
     7741 cattccattt ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag
     7801 cctataagtc aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag
     7861 caaatttcgt gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac
     7921 aaaagaacag aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga
     7981 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
     8041 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
     8101 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
     8161 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
     8221 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
     8281 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
     8341 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
     8401 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
     8461 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
     8521 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
     8581 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
     8641 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
     8701 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
     8761 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
     8821 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
     8881 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
     8941 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     9001 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     9061 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     9121 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     9181 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     9241 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     9301 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     9361 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     9421 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     9481 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     9541 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     9601 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     9661 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     9721 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     9781 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     9841 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     9901 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     9961 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
    10021 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
    10081 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
    10141 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
    10201 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
    10261 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
    10321 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
    10381 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
    10441 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
    10501 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
    10561 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
    10621 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
    10681 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
    10741 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
    10801 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
    10861 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
    10921 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
    10981 aatcacgatc gttgccattt ttaccatgaa aaaacgagac agcttcttac tactttggct
    11041 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
    11101 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
    11161 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
    11221 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
    11281 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
    11341 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
    11401 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
    11461 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
    11521 tccgcctgaa aaacaaacct gtcgaacaag caatcaaaga aacgatccat gccggcggaa
    11581 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
    11641 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
    11701 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
    11761 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
    11821 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
    11881 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
    11941 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
    12001 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
    12061 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
    12121 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
    12181 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
    12241 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
    12301 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
    12361 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
    12421 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
    12481 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt
    12541 ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata
    12601 actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca
    12661 attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt
    12721 gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga
    12781 gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt
    12841 tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga
    12901 cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt
    12961 agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg tggtagatgt
    13021 atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DAJSNV010000072        12750 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSNV010000072 DAJSNV010000000
VERSION     DAJSNV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.075"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON516_002023"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON516_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3345499.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     DCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ON516_002024"
     CDS             complement(322..891)
                     /locus_tag="ON516_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3345500.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ON516_002025"
     CDS             1077..1979
                     /locus_tag="ON516_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3345501.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON516_002026"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON516_002026"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3345502.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON516_002027"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON516_002027"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3345503.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON516_002028"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON516_002028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3345504.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ON516_002029"
     CDS             8547..8732
                     /locus_tag="ON516_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3345505.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ON516_002030"
     CDS             complement(8794..9990)
                     /locus_tag="ON516_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3345506.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ON516_002031"
     CDS             complement(10160..11596)
                     /locus_tag="ON516_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3345507.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ON516_002032"
     CDS             11889..12698
                     /locus_tag="ON516_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3345508.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3806 a         2678 c         2251 g         4015 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tctgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct
//
LOCUS       DAJSNV010000073        12715 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSNV010000073 DAJSNV010000000
VERSION     DAJSNV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.076"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            214..423
                     /locus_tag="ON516_002033"
     CDS             214..423
                     /locus_tag="ON516_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345509.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            483..1154
                     /locus_tag="ON516_002034"
     CDS             483..1154
                     /locus_tag="ON516_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3345510.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1207..3183
                     /locus_tag="ON516_002035"
     CDS             1207..3183
                     /locus_tag="ON516_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3345511.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3196..3951
                     /locus_tag="ON516_002036"
     CDS             3196..3951
                     /locus_tag="ON516_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3345512.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            3948..4772
                     /locus_tag="ON516_002037"
     CDS             3948..4772
                     /locus_tag="ON516_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345513.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5073..7172
                     /locus_tag="ON516_002038"
     CDS             5073..7172
                     /locus_tag="ON516_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3345514.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7210..9267)
                     /locus_tag="ON516_002039"
     CDS             complement(7210..9267)
                     /locus_tag="ON516_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3345515.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9381..9593)
                     /locus_tag="ON516_002040"
     CDS             complement(9381..9593)
                     /locus_tag="ON516_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345516.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9780..10247
                     /locus_tag="ON516_002041"
     CDS             9780..10247
                     /locus_tag="ON516_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345517.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10244..10486
                     /locus_tag="ON516_002042"
     CDS             10244..10486
                     /locus_tag="ON516_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3345518.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10504..10806
                     /locus_tag="ON516_002043"
     CDS             10504..10806
                     /locus_tag="ON516_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345519.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10878..>12715
                     /locus_tag="ON516_002044"
     CDS             10878..>12715
                     /locus_tag="ON516_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345520.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVA"
BASE COUNT         4547 a         1956 c         2541 g         3671 t
ORIGIN      
        1 gtattcaaag ccttatctaa aggaacagat atggaaaaag aaagattgcg aaaagaattc
       61 gattattgga gaacccataa tggaatcaag gatttgaaac cagcacggcc agtaattaaa
      121 cgaaagaaaa agataaccca agactgaagt gtagagcgta tgtgatcata ggctttttta
      181 tttttgcaaa aaacaaggaa ggtaggaaaa ctcatgaatt atgaagaaat cgaaaacaga
      241 aaaaaagtgt cgaaagaaat ggaagaaaag ttactaaaaa cgatgaagca aaaacacctt
      301 aaaagattat ctgtcgcgca atatatcaat gatatgcagt taacaggaaa agagaaagct
      361 tgtttgttag gttcaatgaa aaactttgaa cagttgagac gaacttatag aattcatttt
      421 taaaactttt tctatggaaa tgagaaaaag tttttttgtt ttgtctgaaa ggaggcagat
      481 ggatgaaaaa atggttattt ggttttttag gagtcacttt aatcgtcttt tgcagtgtgt
      541 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
      601 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
      661 tcaataatga tatgtctttg gggcaaatgt tgtccataca aaaagaagca atcgaaatgg
      721 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
      781 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
      841 atgccgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
      901 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
      961 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgggtac tttaaattaa
     1021 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1081 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1141 gagagctaca ataacaaggt tttaatcaac gcgagtcgtt gaaatatgaa atgaggagag
     1201 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttgttgg aatgttagga
     1261 atcattgcag gctgctttca ggggtatcaa gtacatgcag aagatgtcac gcaaaaaaca
     1321 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1381 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1441 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     1501 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     1561 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     1621 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     1681 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     1741 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     1801 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     1861 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     1921 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     1981 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2041 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2101 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2161 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2221 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2281 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagc
     2341 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggtaaagg actttcttat
     2401 gttgctgaaa aagcagatat taaagtaaca aataatgatg gttcagtcgt attaaaagaa
     2461 aatactgact ataaaatcga aaattctgat agtggttttg tgatcgattt tatcgtaaat
     2521 aatggacaag taagtgatac agttgcgaat ttacatggtc aagatctaaa aatgacttat
     2581 aacatgtttg tgaatgatag tgctgaaatc gcaaatccat taacaaatag cgtggatttc
     2641 gtttataaca ataatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     2701 gtgacttatg gcgcaaaatt cttaaaagtc gatagcggcc tatttggaac aggtatcaaa
     2761 gcaacaccgc ttgaaggcgc agaatttgca gctaagaatg cagaaggtaa gtactacggt
     2821 ggacttactg atacagataa agatggtgtc aaagaagcag tttgggtaga cgatgtagca
     2881 aatgcagcaa tcctaaaatc agataaagag ggtcattttg aaatcactgg tttaactgaa
     2941 ggagattact cactggaaga aacaaaagct cctgaaaact atcaaaaatt aacaaaagag
     3001 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaat
     3061 aatcaaaaag cttccgtacc aatgactggt agcaacggtt tccaatctta cgtattgatc
     3121 tcttgcttgt tgctaggagc agggggactt tctgcagttg tttactttaa gaagaaagcc
     3181 tagaaggatc agctaatgat tattcgtaag cccccaaaaa gaagagtcat gcacaaactg
     3241 atgatatttt tttggttgct gtcggcagta gcagtctatt taattccagt tgtggaaaca
     3301 aatgtagcga aatttcaaca acagcaaaga attttagcca ttgaaaatct tgaaaaaaca
     3361 gattcttctt tggatgttgc gaaaatagag ttaggcgatc ctgttggtgt tcttacaatt
     3421 ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat
     3481 ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta
     3541 atcgcggggc acagtggact ttataaagat aatcttttcg atgatttgcc ttcagtaaaa
     3601 aaagggcaaa aattctttat taaggtaaat gaagaacaac atgcctatca aatttatcga
     3661 attgaagaag ttaaagcaga agaattacaa aaaaattatt tagattatct tatgcctgaa
     3721 aaagaccaag ataggatcac gttaatgaca tgcacaccaa aaggtgtcaa ctcacaccgc
     3781 tttttagtgt atggtgaacg agtttccttc agtaaagaag atttaaaact acagaaaaaa
     3841 gcaaaaaata ggaaatcaat ttcattggat tggcgattag cactgctatt cgcttttttt
     3901 tgcgttccta ttatttttat ttataaaaaa ataaggagga gatcggaatg aataagagaa
     3961 aatggctgtt tgtttgtgta gcagctctct tttctactgt agcttattcg caactgtctt
     4021 tcgctgacag cagtgatagt agtaccactc aaacaataga taattcaatt actgcaccaa
     4081 gcgaaacgga cagttcggag aatactacag aagaaacggc agattcaaat ataagcttta
     4141 ctgaatcaac gaatccaacg tcagaaagtt cttcagagac ttcagaaaag ccgcaagacg
     4201 tagaagatac gcaagtgatc aagcaagcca ttcatattca aaaaattatt tcttcgaatc
     4261 aaatcaacaa tcaaggcagt caagtagccc agcaagaagg tatcaatggc gcgaagttta
     4321 ttgtctacga tatcacgaat atcttagaag aaatgtcaca agatgataat gagaaaaatc
     4381 aatctgtaga aataattgag tcaaaattga aagatcgagc aaaaaaactt tcatacgacc
     4441 agttgaaaaa agtggctgaa ggagaaacca aaacgattga tgatcaggaa ggcgtgattg
     4501 aattttccat tgaagtacct gcaaacaaaa aacaagcata ctatatcgtt aatgaatcaa
     4561 gccctgaaaa catttcaaac agtgaagata ttgttttatt gacacctgtt tcaaatgaaa
     4621 aaggtgaatt tttgacggat gtttggttat accctaaatc agagaaatca gagccgaaag
     4681 aagaaattaa aaaagttgtg tcaactggtg taaagaagaa cttttttgaa aattgttggg
     4741 aattttttgc tcaattattt tttgataatt aggatgaaat gatgaaaata ataagaatat
     4801 tagtttttaa tctatccttt ttagccgcaa caatattgtg ttgcggttta attttttctc
     4861 aattatcaat ttatccaaga tatcaacgat tgttcattca ttttctaaac attcaatgtg
     4921 cttccatgcc attagaggtg attaaaaagc tcttgatcaa tccaattttt atggttagtt
     4981 tggttcttct tacaggattg gtaacgtggg tattaggaaa aaaagaatga aaagcttacg
     5041 atcgtttaaa aaaatattag atggagggca ttatggaaaa taaaattcat tgcactacgg
     5101 gaagagtaat tactttagtg ttgtttttgt tccttcttct ttttgcgagg ggaactgcag
     5161 tccaggcctt agaaaaagtt gatccgagtc aaatcaaaga aaataataat ctggtgcttt
     5221 atcatgatag tgattcattg agaaatatcg aagtaactgg gatcaatgga tttaagatac
     5281 tagatgagta ttcaactaaa aaacaaaaag tacttgcttg gccaaaagtc aaagatttga
     5341 aaagtacgtc aaaaatcgtt gcccattatc aaaaagtagg tacttatcta ggaaaagaaa
     5401 ttgattgtga cgtgactttt tctaattttg tagcgcacgc tggggttaac caagacggat
     5461 tagctaaaac aattaatggc aaaaagtatt ataattattt gctctttgat aaaaatattt
     5521 atcgaggatt tttcatgcag tcaatcaatg tcatgaatgc aaaactagaa tttaagtatg
     5581 caggaacaaa aacaaaagta gctttgagtg aaccacagcc agaaattcaa tcaaaaatga
     5641 gatcgatgag agcagctggt attgatttaa acgatacatt tctatccatt tacagtttaa
     5701 atggacagta tcagcaacgt gtgtcttggt ataatgacaa taatgaatct gtcggtgaac
     5761 gtaatattga tatgagagaa tttgttgggt atgaaaaaat gggtagctta ccttattttg
     5821 tcacaacaga tacagcatgt gcagaataca aagctcctgc gttatcaaca aacaatttaa
     5881 cttcaaaagt agtgggagga cgtgcagaaa aggcttatag ctcgaatgat catttcaccg
     5941 atgttgtagg agctgatact tatcacagaa gtggtgtaac gtatacgctt caaggcgctt
     6001 ccccaacatt catgattggc gcaaatacga atagtatgat gtttagcttt gatactgcat
     6061 tgctatggac accacaacca tcgaagccta caaaagaagt gtttaacaaa gctaatactg
     6121 aagaggcagc acacaatatt gacaaaaaag tgattccaca aggatcagat gtttactatc
     6181 atattcacca aaagtttgat gcattaacag tcaacacaat gaacaaatac aaatcattta
     6241 aaatcactga tacctttgac agcaaaaatt ttgatatggt atcggatggg aaaaactatg
     6301 atggcgcatt gtggtatgtt gaagcgaatg gtacttggaa aaaagctgat acaggaggaa
     6361 agttaactgt atcaggtaat acagtcactt atactgcttc tgacagcttt ttagctaacg
     6421 ctgcaaatta cggtagggat tatattttgc agctccatat gagagcaaaa aatgatgcaa
     6481 aaggaattgc accaaatgtg gctactgtaa cgtttaacaa tgatccgaac aaaggaacac
     6541 aaaaaacaaa tacagtagaa aactatttct ctcttaaacc aaataaaaaa gtggaacaag
     6601 atgggaaaga tgttgatggt cgaaatgatg gtaaaaaagg aacgcctaca gctcctttaa
     6661 atgcaggatc tgaagtacaa tatctagtca cacaaaaatg gcatacaaaa ggtgtagaca
     6721 ttgcctcaga tcattacaag caatttagta ttcaagatcc agtggaagat cgtttgacgt
     6781 ataaagaagg ttctgcgcga gtgatagata tgagtacagg aaaagatatt acttcagaag
     6841 gtacactaac ctatgatagc aattcaagaa cgctgaagtg ggaagcttcc gctgatttct
     6901 taagtaaaaa tcttttagat ggacgagaaa ttcagctgat atttacagct aaaactcctt
     6961 tgaatgaaga attggatatc gacaaccaag caaaagtttc agtagaaagt attgagaaag
     7021 caactaatgt agtaacgatt ggggtaaaac ctaacttacc tcagatcatt gtgccaaaaa
     7081 ctggatcgaa tcatttagtg ctaactgtta ttggaagtat attatttctt actttagcat
     7141 cattgggcta tgtagtttta aaactcaaat aaatataaaa acaaggctga gaatcagcct
     7201 tgttttcttt tatttgaaga ttggattcgc actaataaat acattatcta cgccaattct
     7261 accagcagag tcaactacaa aattatgacc taatccgaga atactttggt tattataaag
     7321 taaatttgca ttatgggcag ggctattttt ccatcctacc atggcatcga tagcaccatc
     7381 gtctgataaa aggtcatcaa tatacagccc agtttgactt atgttttctc ccttagcacc
     7441 gtagcagcct aagctttcca ataaatcttg gaaagtacca ccaccagggc gatagtgact
     7501 gaagctgact tgtaattctt tagctcgaat atctgcagcc tgttgaacaa tatcattgaa
     7561 ttgcattggg gcaacaccat tttgttggcg ataatcattt aaccaagcaa gttctttttg
     7621 acgcataaag ttacgtaatt cgtctaatga aatcttttta tatttgaatg tgattgtttt
     7681 attttgatcg acgttattaa tggatcgttg gccgttttta tccacggtaa cggtaggatc
     7741 gtcatttctg cacaagaaat aattttgatc agtcatattt ttccaaggag tagagaagct
     7801 atccccttcg tatactgttt ttgattcact agagagaaca gcgttggtat cagcatttac
     7861 atgattaaca gtcacattaa atttacgagt gtagttgaaa atataatttt gactttcagt
     7921 cacattgttt aaggttactg aatttgttgg ttctacccga tcgtctaaaa catatccttg
     7981 aatagaagga gcagttaaag tgacactttc accgtgagtg acgttgatta tctgttcttt
     8041 caaaatatca actcgcccct catttttgta gttgttattg cacaacaaat tgatttgatg
     8101 cacttgatta taattgaatg tgattgttgt attacctgtg attgttttgc tttgagtatc
     8161 attaccaatc actttgtagc taaaattatc tttagcacct tcgctatatt taaatgcttt
     8221 tgccttagca gtgtaagtag aaccagaagt cacttttgct tgatctgtag caatcacttg
     8281 acctgtttga ttgtcaatat ggttaatggt aaccatatat tcttttggcg tttcaggttt
     8341 gtgttgcaac cctttaaatg cagcatcaag actattttta gcattgttta cttgtgtttg
     8401 agaaacctta gaatcgtcta gtactttttt agcattggat aaagcagtct gaaaattatt
     8461 ccaagattct tctgtaaaat ctcctttagc agttgatttt actcggttgt ataaagcttg
     8521 taattcagct ttattcacag taggaactgg ttttttcttt aatcctttgt aagcagaatc
     8581 taatttattt ttggcactat ccacttgctt ttgagtcgct tttgagtcat ttaacacttt
     8641 cttagcattg tttaaagcgg tttggaatgc gttccaggta ttgtcggtat agtcaccttt
     8701 tttcgttccc ttaacgctcg tataaagagc ttctagagcc gatttattca ctttgggttt
     8761 agaggtgtca gcgccatacc aaccgatacc ttcgtatttc cagccatagt ctttggttaa
     8821 ccattgtact tctttccaac ttgcagtgta gtgatgtgag ccagatttta caccaggaag
     8881 gaaaaggcgg tagatcggtt tattattttt ttcggcagaa cgccaggcag gcccttcata
     8941 agtccagccg taatttttag tcaaccattt cacttcatcc caactagctg tgtaatggtg
     9001 atcacgaaga cctttattgt acaaacgata gacaagatgg ccaccgctag tcggtgcttc
     9061 ccaggcgggg ccttcataat ttccccattt tatctttact agatggtcac gttctttttt
     9121 gctagaggtg taaaaatgtt cattattatt tggattgtaa aggcgataca tgacgttagt
     9181 tgaagcagcg tcaacagatc cagcaagact aagtgaggca gcaaatgttc caatagctaa
     9241 aacagttttt ggattaaata atttcatttt ttcaactcct aaatttaatt aaaaatgttt
     9301 acatgattat tatatatcgg ctaaccgata tagtcaataa aaaataagaa aagccttata
     9361 taattttggc ttttcttatt ttatttgtta gttgaagtat taatatcaac gatttcttca
     9421 gccaatttat ttaatttcat agcatttatg aatctcatgt tgtctatact agagcgtttg
     9481 ttggcaaggt cacttactgt agttaagggt acgccagatt ctatggatat agaacgaact
     9541 gatttaccac tatcaaacag caaccattcg attttctttt tatcagcgta catttcaatg
     9601 ctccttcttg ttttttcttt attatagctc ttttttgtaa tgaatcacaa tatcaagaga
     9661 aaaattttct atagaaaggt ggatttttat gagtattcgt tctccgtgta tccaaatgag
     9721 gtttgttgct actttcgtta tttgatatac taaaaataaa caacgaaaga aggaataaaa
     9781 tgaagaaaaa gcaactatgg tttgtctcta tgtttgcaat attttttatt attttaagtg
     9841 gatgttcaag taataagaaa tcggaagcta aagaagataa tagtttattg caaccaggaa
     9901 ctgtttggaa agaagaagta ggtggtctta ttcataattt aaaaattata gatgatacta
     9961 cctgggaata ttctgaaagt gtatggcatc cagatcctgt acaaataaca gttaagaggc
    10021 aaaaagacta tgaaggttta gaaagataca aaatagtaga ttctgcaggc attagggaat
    10081 ttataaacaa acctgattca ttatttattg ttgttcccta tgagaaggat ggcgtaaaaa
    10141 aaataatttt tcttggatct agcaaggatg agaagcgaac gaaagagaaa ttaattcatg
    10201 atggatcaga gttaaataaa tacaaattac aaaagactag tgagtgattg ctagtctttt
    10261 tatttatcct gaaggaggaa tcacagtggc aaaagttaaa acaccattcg aaaaagccgc
    10321 gtctaaaatc ttagaacgcc gtggtgtttc tgaaatacag tggaaaaaag aagttcttga
    10381 aaaagcacaa tttgacctat tacgaggaaa agataaggac ttagaagaat atatctatca
    10441 acgtgaacgt gaaaaacttg tattcgatga aattattaaa cattaaaagg agcgaataat
    10501 cttatgtttg aatcaattac aatgaatttt ttaactcaac cgaaagtagt agctgcagtt
    10561 gatattttta gagcattaca aactgccgca actagtttga aaaataatct gatgattttt
    10621 gctgctgttg cggcagtggc ttccattgct cttggaggta ttttcttctt ctttgggcga
    10681 agaggagctg aaaacgggaa gacacacgta ggttatgtat tgttgggggt aggagttatt
    10741 ttagctgccg cttctattgt agtttcaatg ttctcaatgt ttggacaaac cccacaagga
    10801 ctataaaaaa aacaagtagg aggagtaaaa agtacgtggc tactttttac tccttttttt
    10861 atgaaaggag aatattcatg ttttttacga tttcaggaaa tgcaaaattg gataaagagt
    10921 taataagaaa agcaacgaag aaagtgggaa agcgtacttc ctttttaccg aaaacaatta
    10981 aatattcggt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg ttagaacaag
    11041 agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga aaaatagaaa
    11101 aggctggcac agaatacgtt tttggcccac tggattatca aatttcagaa ggtttttatc
    11161 ctacattgat agatttaatc cgccttgatg tgtttaaaga tacggatgaa actatgagtg
    11221 tggaagagtc ttatcgaaac gaaaaacttt tacaagtttt agaagaagag ttagagagcg
    11281 accaaagaaa agtgaatcaa gtgagtgaag tcgaagtaaa tgaagaagta ccatgggaac
    11341 gaccaatatt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag
    11401 ctgatattcc gactcagaaa tcgatatttg aggaagagaa cgatgtgatg ggtcaattgg
    11461 aggtagatcc ctttgaagaa acaatggagg atgaagagac ggacgaagta gaagttgatc
    11521 cagttgaagc taatgaagag tcaacatgga ctgaagggga ttttcaggaa tatattgaac
    11581 cacttatcag tgaagaagta gaaaaaaacg aaacagagat acaaaaggca actgtatttg
    11641 attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aacaaattat
    11701 ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga
    11761 gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta gagtctgtcc
    11821 aatcaacaaa ttggcatgag cttggacaag aagatttatt agccaccttt gaagaacgtc
    11881 aacaagaaac agatcaagaa attaatgagt atgcgacgga acagtcgtct aattgggaga
    11941 aagtaaaaga ggaactaaat aaagaagaac agcgagtgat tgaagaagaa gtacaaaaag
    12001 tcaaagagcg gtacgaaaag aaacgcgaca atgcttattc cttgtgtgta gaaaagatca
    12061 acaattttac taagacgaat caagaagcgt tatcgaaaga aaaagaaaac ttgttaaaag
    12121 aacgtattaa agaacgaaaa gaagagttct accgcggttt acgtacagat aagctaaaga
    12181 ttagcaaaga attaaatgaa caactgaata agctatatca aacaacgact gaagttctta
    12241 tggaaaaaca cgaacagatc cagcaagaat tacaagtaca aattccaaaa tggaaaaaag
    12301 agtatgaaat tcaacaacaa gaattgaaag aacaagaaga aaaacaacgg caaaaagaaa
    12361 aagagcgtga acaattagaa ataagaaaac gggaacagga tcttgcggaa caaaaaatgg
    12421 cgatagaaaa acaacggtta gagcaagaaa aggaaaaaga agcagctgct cgtaaggaac
    12481 gtcaagaaca aatggataca ttgcgtcaag cacagttgaa ttatctagca caacctcaac
    12541 cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa tttaactcct tcaccccagc
    12601 gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag
    12661 gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct gtagc
//
LOCUS       DAJSNV010000074        12647 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSNV010000074 DAJSNV010000000
VERSION     DAJSNV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.077"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..287
                     /locus_tag="ON516_002045"
     CDS             <1..287
                     /locus_tag="ON516_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345521.1"
                     /translation="NSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPP
                     GINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(432..1172)
                     /gene="truA"
                     /locus_tag="ON516_002046"
     CDS             complement(432..1172)
                     /gene="truA"
                     /locus_tag="ON516_002046"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3345522.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1363..1998)
                     /locus_tag="ON516_002047"
     CDS             complement(1363..1998)
                     /locus_tag="ON516_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3345523.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2145..3386)
                     /gene="sstT"
                     /locus_tag="ON516_002048"
     CDS             complement(2145..3386)
                     /gene="sstT"
                     /locus_tag="ON516_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3345524.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3584..4582
                     /locus_tag="ON516_002049"
     CDS             3584..4582
                     /locus_tag="ON516_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345525.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4595..6613
                     /locus_tag="ON516_002050"
     CDS             4595..6613
                     /locus_tag="ON516_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3345526.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6673..7389)
                     /locus_tag="ON516_002051"
     CDS             complement(6673..7389)
                     /locus_tag="ON516_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3345527.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7616..8158)
                     /locus_tag="ON516_002052"
     CDS             complement(7616..8158)
                     /locus_tag="ON516_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3345528.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLVQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8163..8390)
                     /locus_tag="ON516_002053"
     CDS             complement(8163..8390)
                     /locus_tag="ON516_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3345529.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8469..10349)
                     /locus_tag="ON516_002054"
     CDS             complement(8469..10349)
                     /locus_tag="ON516_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3345530.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10538..11152)
                     /locus_tag="ON516_002055"
     CDS             complement(10538..11152)
                     /locus_tag="ON516_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345531.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11294..11854
                     /locus_tag="ON516_002056"
     CDS             11294..11854
                     /locus_tag="ON516_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3345532.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12159..12473)
                     /locus_tag="ON516_002057"
                     /pseudo
     CDS             complement(12159..12473)
                     /locus_tag="ON516_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         3955 a         2507 c         2007 g         4178 t
ORIGIN      
        1 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
       61 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
      121 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
      181 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
      241 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
      301 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
      361 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
      421 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
      481 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
      541 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
      601 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
      661 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
      721 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
      781 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
      841 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
      901 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
      961 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     1021 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     1081 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     1141 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     1201 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     1261 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     1321 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     1381 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     1441 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     1501 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     1561 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     1621 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     1681 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     1741 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     1801 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     1861 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     1921 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     1981 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     2041 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     2101 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     2161 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     2221 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     2281 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     2341 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     2401 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     2461 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     2521 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     2581 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     2641 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     2701 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     2761 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     2821 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     2881 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     2941 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     3001 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     3061 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     3121 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     3181 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     3241 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     3301 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     3361 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     3421 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     3481 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     3541 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     3601 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     3661 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     3721 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     3781 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     3841 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     3901 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     3961 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     4021 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     4081 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     4141 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     4201 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     4261 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     4321 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     4381 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     4441 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     4501 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     4561 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
     4621 ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat
     4681 tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta
     4741 ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc
     4801 aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc
     4861 acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc
     4921 caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc
     4981 gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct
     5041 gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt
     5101 gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta
     5161 ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt
     5221 ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt
     5281 taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat
     5341 tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga
     5401 aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag
     5461 aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc
     5521 tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat
     5581 aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat
     5641 ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc
     5701 ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga
     5761 aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa
     5821 atttggtttt gacaggttct attatttaga cagtccggat aaattaactt atacggataa
     5881 aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt
     5941 acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc
     6001 ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa
     6061 tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa
     6121 aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga
     6181 ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca
     6241 gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa
     6301 agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt
     6361 aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga
     6421 tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag
     6481 tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg
     6541 tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa
     6601 aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc
     6661 ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc
     6721 attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt
     6781 caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata
     6841 tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc
     6901 actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt
     6961 caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact
     7021 tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga
     7081 tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga
     7141 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
     7201 tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct
     7261 agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga
     7321 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
     7381 ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
     7441 atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga
     7501 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
     7561 gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
     7621 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
     7681 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt
     7741 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
     7801 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
     7861 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
     7921 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
     7981 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg actaatttca
     8041 tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat
     8101 gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg
     8161 atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca
     8221 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
     8281 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc
     8341 atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt
     8401 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
     8461 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
     8521 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
     8581 aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt
     8641 tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa
     8701 ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata
     8761 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa
     8821 gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat
     8881 aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga
     8941 ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt
     9001 tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg
     9061 attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc
     9121 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
     9181 tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct
     9241 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
     9301 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
     9361 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag
     9421 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
     9481 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
     9541 ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca
     9601 acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat
     9661 ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag
     9721 gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt
     9781 ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg
     9841 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
     9901 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt
     9961 ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac
    10021 tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta
    10081 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    10141 agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat cataataggt
    10201 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta
    10261 aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    10321 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga
    10381 gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa
    10441 ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa
    10501 gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca
    10561 gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta
    10621 gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga
    10681 atatttcttt tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt
    10741 ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct
    10801 ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag
    10861 caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat
    10921 gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat
    10981 acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa
    11041 gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat
    11101 agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc
    11161 ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg tctactatct
    11221 ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata
    11281 aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc
    11341 tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact
    11401 aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg atatctatga
    11461 tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc tctcttatga
    11521 tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt acgcaaatac
    11581 ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga agaaagttaa
    11641 acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga aaacgattat
    11701 cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat ccgttctcaa
    11761 tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac tcttacagaa
    11821 actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag cttagttaag
    11881 aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg tttactgaag
    11941 attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt agtgctgctt
    12001 ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc attaataaat
    12061 tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt tatagtcata
    12121 ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc attttcaagg
    12181 ggaatcagct catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
    12241 aatggtttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
    12301 gctttcatat taggaattgg atcttcacct ggcatatatt gcagattaga agcatgtaat
    12361 tttttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc acttacttgt
    12421 gtaaaatagc gacgtttaat gacaaagata tcttcattgc tatctagtgt accttcattt
    12481 ctttgtgctt aaattcaaag ataattttct caataaagat ttttgattcc agtaagattc
    12541 ctttactcct tttacaacat agcgttgtcc tttgttttga aacatcttct tcccctcttt
    12601 gccattaaaa atagcccaat tctctataaa gaagattaag cttcatt
//
LOCUS       DAJSNV010000075        12572 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSNV010000075 DAJSNV010000000
VERSION     DAJSNV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.078"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..701)
                     /gene="plsY"
                     /locus_tag="ON516_002058"
     CDS             complement(63..701)
                     /gene="plsY"
                     /locus_tag="ON516_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3345533.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            828..1262
                     /locus_tag="ON516_002059"
     CDS             828..1262
                     /locus_tag="ON516_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3345534.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1428..3473
                     /gene="parE"
                     /locus_tag="ON516_002060"
     CDS             1428..3473
                     /gene="parE"
                     /locus_tag="ON516_002060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3345535.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3485..5935
                     /gene="parC"
                     /locus_tag="ON516_002061"
     CDS             3485..5935
                     /gene="parC"
                     /locus_tag="ON516_002061"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3345536.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6212..8443
                     /gene="pflB"
                     /locus_tag="ON516_002062"
     CDS             6212..8443
                     /gene="pflB"
                     /locus_tag="ON516_002062"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3345537.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8687..9448
                     /gene="pflA"
                     /locus_tag="ON516_002063"
     CDS             8687..9448
                     /gene="pflA"
                     /locus_tag="ON516_002063"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3345538.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9533..10459
                     /locus_tag="ON516_002064"
     CDS             9533..10459
                     /locus_tag="ON516_002064"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3345539.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10641..11534
                     /locus_tag="ON516_002065"
     CDS             10641..11534
                     /locus_tag="ON516_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3345540.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11668..11793
                     /locus_tag="ON516_002066"
     CDS             11668..11793
                     /locus_tag="ON516_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3345541.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11927..12559)
                     /locus_tag="ON516_002067"
     CDS             complement(11927..12559)
                     /locus_tag="ON516_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345542.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         4514 a         2130 c         2639 g         3289 t
ORIGIN      
        1 aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg aatgaaaaag
       61 tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc agtatggttt
      121 ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac taaaaaggca
      181 atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata aggaaggacg
      241 atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac catacttgta
      301 caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg attaaatcct
      361 aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc aaaaatcgga
      421 aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc agaaaggtgg
      481 aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag caggacaaca
      541 atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt accgctcccg
      601 tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga aggaatagaa
      661 cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc tctttcaagt
      721 tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa aaatgaaaga
      781 tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa
      841 atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag
      901 gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg caagacaatg
      961 gatataaaat cataccagta aatccattat tggctggtga agaaattcta ggcgagaaag
     1021 tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta tttagacgga
     1081 gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg
     1141 cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca ggacgtacag
     1201 atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa aaccaaaaat
     1261 aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag cggttaggag
     1321 ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc tagtatgata
     1381 atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag
     1441 taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag gcagttagaa
     1501 aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat ttagtctatg
     1561 aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag attgatgtga
     1621 ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg cctgtaggaa
     1681 tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat gctggcggaa
     1741 agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga gccagtgtgg
     1801 taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta gagtatcagc
     1861 agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga aaaacaaaaa
     1921 aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt acaacgaaat
     1981 tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta aaaggcgtta
     2041 aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt cattacgaag
     2101 aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg acaccagttg
     2161 tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg
     2221 gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat gggggaaccc
     2281 atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg agaaaagttg
     2341 gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa gggttagctg
     2401 ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa
     2461 aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa caaatgggct
     2521 tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca atcaaagcca
     2581 gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta
     2641 aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga aaccctaaga
     2701 aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa caaggaagag
     2761 acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc gaaaaagcta
     2821 aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg attggtgcag
     2881 gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc atcatgaccg
     2941 atgcggatac agacggcgcc catattcaag tattactgct tactttcttt tatcgttata
     3001 tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta tataaagtat
     3061 ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac gaattggcag
     3121 caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga cttggagaga
     3181 tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg ttgatccgcg
     3241 tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg ggtgacaaag
     3301 tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca
     3361 gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat gatttattag
     3421 atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa taggaggtca
     3481 cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga tgggtgaccg
     3541 cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata tccgtgatgg
     3601 attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca atacgtatga
     3661 caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt atcatcctca
     3721 tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga agttgcggga
     3781 agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac ctgctgcgat
     3841 gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag atatcgagaa
     3901 agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa cagtccttcc
     3961 agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg gatatgcaac
     4021 agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata tgatcgatca
     4081 tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact ttccaacagg
     4141 agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt
     4201 tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc aaatcgtgat
     4261 cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg atgagatccg
     4321 tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata gaacaggtct
     4381 gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga attatttgtt
     4441 caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg accatatgac
     4501 tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc gaaagcaagt
     4561 cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc atatcgtaga
     4621 aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa
     4681 agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag aagaacaagc
     4741 agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca cagagcttca
     4801 aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc tttcaaatga
     4861 caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc
     4921 cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata cgcaagtctt
     4981 agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag
     5041 ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag gcgactactt
     5101 gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa ataaagcaaa
     5161 tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg ctggtgagca
     5221 catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg ttttcccata
     5281 tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat tgatcaagca
     5341 aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc tcagctgtat
     5401 gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag atcagccagg
     5461 cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt taacagatgt
     5521 tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag atggagatag
     5581 gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa tcgttactca
     5641 gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac gtgcgaagcg
     5701 gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct atatgtcgga
     5761 aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc
     5821 aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg atgagaaaaa
     5881 aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt
     5941 gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc tttttcattc
     6001 atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat
     6061 gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa
     6121 aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt
     6181 acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac
     6241 caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg
     6301 cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt
     6361 gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat
     6421 cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt
     6481 cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat
     6541 ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt
     6601 cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg
     6661 tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat
     6721 catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa
     6781 aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga
     6841 agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg
     6901 ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg
     6961 atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc
     7021 attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc
     7081 acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc
     7141 tgaatataac caattattct caggattccc tatctgggct actttatcca ttgcaggtat
     7201 gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac
     7261 aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga
     7321 aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa
     7381 tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc
     7441 aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac
     7501 actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga
     7561 atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa
     7621 tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc attatatgca
     7681 tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac
     7741 atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata
     7801 tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg
     7861 cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt
     7921 ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac
     7981 ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga
     8041 tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca
     8101 aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga
     8161 tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag
     8221 aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa
     8281 tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc
     8341 gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca
     8401 acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag
     8461 tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag
     8521 tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa
     8581 ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca
     8641 gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac
     8701 aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt
     8761 tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg
     8821 gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt
     8881 cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca
     8941 gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt
     9001 ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt
     9061 gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg aagagcataa
     9121 aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat
     9181 caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata
     9241 tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct
     9301 tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga
     9361 agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga
     9421 agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct
     9481 ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa
     9541 aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt
     9601 ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa
     9661 tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc
     9721 agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat
     9781 ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt
     9841 tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat
     9901 tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct
     9961 ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga
    10021 agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga
    10081 aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga
    10141 tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga
    10201 ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa
    10261 agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga
    10321 attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac
    10381 caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt
    10441 gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg
    10501 attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag
    10561 tcaattatga ctcttgttct tcctcctttt tacgtaaaag ccttttaagt aaaataaaac
    10621 ggatagaaaa gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa
    10681 aactgaccga tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac
    10741 aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc
    10801 ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact
    10861 ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag
    10921 ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt
    10981 gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc
    11041 gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt
    11101 cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc
    11161 ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt
    11221 acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc
    11281 gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat
    11341 cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat
    11401 tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc
    11461 tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga acagttttag
    11521 tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt
    11581 tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc acctattaga
    11641 ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca
    11701 aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag
    11761 tatggcattt tatgggaaga actagctgat tagattagga agtccatttg ataatccaga
    11821 atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta
    11881 gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat
    11941 gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga
    12001 gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag
    12061 caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga
    12121 agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata
    12181 aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa
    12241 aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta
    12301 acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc
    12361 aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat
    12421 ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac
    12481 atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg
    12541 ttctacgttg aatttccata atatctccaa at
//
LOCUS       DAJSNV010000076        12457 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSNV010000076 DAJSNV010000000
VERSION     DAJSNV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.079"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..154
                     /locus_tag="ON516_002068"
     CDS             <1..154
                     /locus_tag="ON516_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3345543.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="ON516_002069"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="ON516_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="ON516_002070"
     CDS             complement(1613..2455)
                     /locus_tag="ON516_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3345544.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="ON516_002071"
     CDS             2780..3196
                     /locus_tag="ON516_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3345545.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ON516_002072"
     CDS             3193..3663
                     /locus_tag="ON516_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345546.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ON516_002073"
     CDS             3677..4450
                     /locus_tag="ON516_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345547.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ON516_002074"
     CDS             4464..5276
                     /locus_tag="ON516_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3345548.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ON516_002075"
     CDS             5330..6295
                     /locus_tag="ON516_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3345549.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="ON516_002076"
     CDS             6432..9155
                     /locus_tag="ON516_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3345550.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="ON516_002077"
     CDS             9168..9800
                     /locus_tag="ON516_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3345551.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="ON516_002078"
     CDS             10005..10232
                     /locus_tag="ON516_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR3345552.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="ON516_002079"
     CDS             10301..10567
                     /locus_tag="ON516_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3345553.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="ON516_002080"
     CDS             10557..10826
                     /locus_tag="ON516_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3345554.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="ON516_002081"
     CDS             complement(11005..12306)
                     /locus_tag="ON516_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3345555.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4065 a         2015 c         2444 g         3933 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg gagcttctgt
    12361 atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc agtcctagaa
    12421 attatagagc cttttttatt tggtcttgaa gaagaaa
//
LOCUS       DAJSNV010000077        12331 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSNV010000077 DAJSNV010000000
VERSION     DAJSNV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.080"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..325)
                     /locus_tag="ON516_002082"
     CDS             complement(<1..325)
                     /locus_tag="ON516_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3345556.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIP"
     gene            959..1930
                     /locus_tag="ON516_002083"
     CDS             959..1930
                     /locus_tag="ON516_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3345557.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2008..2244)
                     /locus_tag="ON516_002084"
     CDS             complement(2008..2244)
                     /locus_tag="ON516_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3345558.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2213..2344)
                     /locus_tag="ON516_002085"
     CDS             complement(2213..2344)
                     /locus_tag="ON516_002085"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3345559.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2420..2803)
                     /locus_tag="ON516_002086"
     CDS             complement(2420..2803)
                     /locus_tag="ON516_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3345560.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2836..3621)
                     /locus_tag="ON516_002087"
     CDS             complement(2836..3621)
                     /locus_tag="ON516_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3345561.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3799..4899
                     /locus_tag="ON516_002088"
     CDS             3799..4899
                     /locus_tag="ON516_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3345562.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4889..5713
                     /locus_tag="ON516_002089"
     CDS             4889..5713
                     /locus_tag="ON516_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3345563.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5710..6525
                     /locus_tag="ON516_002090"
     CDS             5710..6525
                     /locus_tag="ON516_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3345564.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6522..7352
                     /locus_tag="ON516_002091"
     CDS             6522..7352
                     /locus_tag="ON516_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3345565.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7359..8390
                     /locus_tag="ON516_002092"
     CDS             7359..8390
                     /locus_tag="ON516_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345566.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8497..9770)
                     /locus_tag="ON516_002093"
                     /pseudo
     CDS             complement(8497..9770)
                     /locus_tag="ON516_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9997..10182
                     /locus_tag="ON516_002094"
     CDS             9997..10182
                     /locus_tag="ON516_002094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345567.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10306..11022
                     /locus_tag="ON516_002095"
     CDS             10306..11022
                     /locus_tag="ON516_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3345568.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10924..11444)
                     /locus_tag="ON516_002096"
                     /pseudo
     CDS             complement(10924..11444)
                     /locus_tag="ON516_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11732..>12331
                     /locus_tag="ON516_002097"
     CDS             11732..>12331
                     /locus_tag="ON516_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3345569.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQ"
BASE COUNT         3566 a         2278 c         2800 g         3687 t
ORIGIN      
        1 gggggatttt ctcctcgaag gagtagccta aaaaccagcg gtaagcgaca ttggtatcga
       61 cttccttaat ggtttgtcgc atggaacgga taccgaataa atactgaata aacaccaatt
      121 tgatcagaat cactggatcg atacttgggc gacctaaatc ggagtaagtg gcttcgacga
      181 ttggataaat aaattcaaaa tcaagcgcgg catcaatctt tcgaacgaga tgatcttgag
      241 gaactagatc atcaatcgta agcatcgcaa attgatttct accattcgta ttgtttttag
      301 acatcatagc tatcactcct tgcattcgtt ttcataaatc tattataaaa gaaaagactg
      361 tagacatcat ccataaaatg gactttgtct acagtctgga taacgtgatt ttttgctgta
      421 atgacaaggt tttagctgtt tcagatacca tgtatccaaa aatagtttat tgagaaaata
      481 ggagagtatt tgatactaac tagtaggaaa aaatgagata aaaagtaaat ggaatcattt
      541 aaggtaatct gaaataaaaa atatacagcc ctgtggtact ttacctacaa ggctgtatat
      601 attttttact ctcagcgttt ttgactccgt gtctgactcc gttttattga ttttgaatgt
      661 tattcaatga ccatttaaca ggtcaaattt gatgtttttg attccgtatg acgccgtttg
      721 atatctagtg atatagtgat ttgagtctac atcgaataag ggaataaacc aataaaaaat
      781 gatttagaat attaaaataa caaaaaagag gactacctgt attagtcctc ttttttgacc
      841 agattttgac cagtctattt tacatcctag tatatttata tgagctaatt ttatgattaa
      901 atggtaagtt tttccttgtg tgttttagtt taaactagtg tgtaattcaa aaatctatat
      961 ggaaggaaca gtattaagaa tagaacaggg ttctctacat gatggtgctg gtttacggac
     1021 cgttgtttat ttaaagggct gcccattacg ctgtgcctgg tgttcgattc ccgaatcaca
     1081 aagcaagcag attgaaaaag gatttggcca gaccatgacc gctgaagagg taatggatga
     1141 gattgagaag gatgctgttt tttactttca ctcggatggc ggagtgacca ttagcggagg
     1201 cgaggcactg gttcaggcgg attttgctaa agaaattttg caaaaatcga aatacattgg
     1261 aatcaatacc gtactggaaa ccagcttttg tggtgcttat aatgaaatcc aaaaggtagc
     1321 tccctatgtg gatacattgt ttgtggatgt aaaaatgttt accagcaagc tccacaaaca
     1381 atggaccggc ttagataacc aacaaatttt gaagaatatt cgtcgctttt taattgagta
     1441 tcctaactgc gaagtgcgaa ttcgagttcc tgtggttcca ggtatcaata tgaacctgac
     1501 cgagctgctg acgattgctt gttttgtggc agatcttgat cggttcgtac cgttagaatt
     1561 actaccgtac cactgctatg gtatgcacgg ttaccaagca ttgggactgg agtatccgtt
     1621 ggcagacacg ccagcgcctt cacctaaaga aatgtttgca ttggcagatc aactggctcg
     1681 cacggttcct catttgctgg tcacagcttt aaccaaaaca tttatttatt tattgaatcc
     1741 aagctttatc ttatggataa atgaaagaca ctcactagct gcagccagct caagaataga
     1801 gcttttgctg aagtcagtga gtgtcttttt acgatgtttt ttcgaagtta atttgtcgaa
     1861 tgaacactgg ctcaatcggt acatcttcac tgtttgtcgg ctgagctgcg atcgctgcta
     1921 cgatttctaa cccttggtat acctgtccaa aaacagtata acgaccatcc agctccggta
     1981 gtcctccaag ctccaagtat gcttgcttta cattagcagg tcagcctgcg cctttgtgtt
     2041 ttgcacaata aaaaactggt tgccgttggt gtgggggccg tggtccccta aggccaaggc
     2101 gccattaaaa tgataaagtc cgcgatgcac ttcatcagcg aaaaagccct catagctgga
     2161 aacagcttca tcaccagaac catccaaagc gccaccttga ataacaaatt ccttaatgac
     2221 acgagccgaa aggtgtgttg tcataaacgc cttgctcagc tagggagcgc cagttggcta
     2281 cagccagggg agcttggtca ggaaataagc ggagacgaat ttcaccagca tcggtgacca
     2341 gcacacaaac ttcttcctgc tgcggttttt ctaattgaaa gagaggaagg tttgcaactg
     2401 aaacgccttg atcctgccat caaatatcga aaatccgcca ctcgaccttc gcctgatcac
     2461 caaggcgagc cattcccaat aaaacggtat aggcggtttc atccgcttga aaggtcaatt
     2521 gcagctgtcc tcgtaaatcg ttgtagtcat ctggggcggc tacttgataa aaaagatgaa
     2581 actgcgcatg caaatattcc aaaggctgat ctttaaaggc tgcaaaataa gcctgaactt
     2641 tttcccgctg caagctgtca gccaccaaaa aattttccag ttgttgtttt ggaacggctg
     2701 ctaacaagga tcggcaaacc gcctcaattt tcaacgcatc ccaatgtcgc gcaattaaat
     2761 aatggatggg tgcctcttga ttgtagtccc acaggttgct catttttttc accactttct
     2821 tctattatat aggaatcaca aaatcggcag cggcgtcagg cggcgttctt ttccttgaat
     2881 gactagcaac ttttctacct gacagtctgc caacagctga ctcatttttt taaagcccag
     2941 ctgataatct tcggcaacat gagcatccga gcctaaggta aagagctttc cgccgacact
     3001 ttgatacaaa ggaacggcat aacggtaaag gtcttcgttg ccgtatttca agaagctctt
     3061 ggcatttaac tccatcgcca attctcgttc aacaaccttt ttgaaaatcg ttagcagctg
     3121 agcctcaaaa tgctccgcga attcttccgc tgtgaaggtg aagcggcgta atccataatc
     3181 aaagtgagtc aaaatatctc cttctggaaa atccgtcaag acctgcaaca tctgatcaaa
     3241 atattccttg gtagtttgaa tgggatcttt ggttaaaacc acatcgtcca tataatcaaa
     3301 gcggccattt tgatgaatac ttaccagctt taaatcataa ggatgctgag cgaggtaatc
     3361 cacgatccgc tcttcttgtc ctggaacaac gccaatctcg atacctcgca gaaatttggt
     3421 ggaataggtt tcttttaaag agtccagttt gtttatatag gctggataat ccggaatgtc
     3481 atcttttccg gtagatggat tgtttaaatc aaagtgatcc gtagaaacga aaaactccgg
     3541 ctgataggct aaatagtttt caaatttttc ctctgaatca aaggaaaaat acgtgtggag
     3601 atgctgatca taatactgca tagggagcct ccttttttct actatcatac cgcaaaagct
     3661 ttagaaggtt aacgggaatt cgtcgaaatt tacaaaccat atacaacctt tacattgttc
     3721 atgaaacctt ttcaagaaac tgattctctc attacaaagc tttgttagaa tggaattatc
     3781 gaaaggggag aagaatgaat gaaaatgaat gaaaaaattc gtaacatgac tatcggttta
     3841 ttttgtgcag caacagtaag tatcctgctg acaggctgcg gctcaggtga agcaacgaag
     3901 caatcggctg cggcagacga ctctttagat acgattatag aaaaagccaa ggaagaagga
     3961 gagattgcca gcttgggaat gccggatact tgggcaaatt gggtaggcac ttggaacgac
     4021 cttgaaaaag aatacggctt aagtcacgtg gatacggata tgtccagtgc agaagaattg
     4081 gctaagtttg aatcagaagg aaaaaatgga accgccgata ttggtgatgt cggtatcagc
     4141 ttcggtccgt tggctgaaag caaagagtta accttacctt ataagaccaa ctattgggag
     4201 gaaattccgg attgggcaaa ggatgatgat ggggattggt tgttaagcta tacgggaacc
     4261 atttccttta tcaccgataa agagaatgtg aagcaagcac cgactagctg gaaggagctg
     4321 caagagggtg actatcaagt tagcgtgggg gatgtgacga ttgccaacca agcacaattt
     4381 gctgtattgg ctgcggcgat tgccaacgga ggtgacgaaa aggatattca gccaggtctg
     4441 aattactaca aaaaaatggc agaaaaaggc cgtttgtcca ccgttgatgc cagcgtagca
     4501 aatcttgaaa aaggtgaaat cgatgtggca gttgtttggg acttcaatgg actaaattat
     4561 cgtgaccaaa ttgactccga gcgttttgag gtaactgtgc caacagacgg ctccatcatt
     4621 agcgggtata caaccttaat caataaaaat gcccctcatc ccaatgcggc aaagctagca
     4681 cgggaatata ttttatctga tgaagggcaa atcaatttgg caaagggcta tgcgcgacct
     4741 attcgcaagg tagagctgcc taaagaagta gctgaaaaat tactgccgga ggatgcttat
     4801 gaagcggcgg tgccagttga agacaatgaa gcttggaaca agacctctgt agagcttcca
     4861 gagctttggc aggcggaggt actgacctat gcaaagtaga cttgacaaac tgattgtagt
     4921 tatactagac ggctgtcgct acgacacagc cgtcgcccag cttgggttca tgaatcattt
     4981 agttgagcac cagcaagcaa gcttattgaa ggtaaaaagt gagctgccca gcaattcacg
     5041 tccgttatac gaagtactga tgaccggcgt tccagcctat gaaaatggca tctatagtaa
     5101 ttaccatgct cagcgatcca aagagtggtc gctgtttgaa ttagttaagg aagctggcgg
     5161 aaaaacagga gccgccgctt accactggta cagcgagttg tacaatcaag ccccctataa
     5221 tcgtttggtt gatcgcatcc agcatcagga ggagcgactg attcagcatg gcatctttta
     5281 tagtgaggac acttaccctg attcgcattt atttgcggat gcccattatt tagtgaatca
     5341 gtatcagcca gactttttgg tcgtacattc gatgaatatt gatgatgtcg gtcacaaatt
     5401 taccgccgat tctcatgaat atgcagcagc ggttaatcag gcggacagat tattggctat
     5461 ttaccttcct gaatggctgc aaaaaggtta tcaagtcgtg gtgactgcgg atcacgggat
     5521 ggatgcgcac ggactgcatg gcggcagctt ggctgcacat cgtgaagttc ctttttattt
     5581 gatttcaaat aaacgccaaa agctgaagga acaaactgta ccacagctgc tggcggctcc
     5641 attgttttgc tggttattag gaattgcacc gtcagagaaa atgcaggaca ttcaagaatt
     5701 gatgagggga tgaaagagat gaaaaaacgc ttggttctgg ttcctgcctc ctttatcttc
     5761 tgtgcatttt tactgctgcc gctgcttttt atggttctgt gcagcttgca cagtgattta
     5821 actaaccagt ggacattggc caattacagt gatattttca gcaacaaata ttacacacag
     5881 gcctttgtta acagcgtact gattgctgtc agctccagtg tgattgggtt aatcgggacg
     5941 tttattttat gtttatgctt aatgaatcta tcgactaaat tgcaggagaa aatcaccttt
     6001 gtttccaatt tggcagctaa ttttgctggg attccgctgg cattctcttt tattattttg
     6061 ttggggaatg taggtgtgct gaaaatgatt ttgccatttc tagcggactt caatttgtat
     6121 tcttggtggg gattgaccat cacgtatacc tatttccaaa ttccgttagg ggtcttgttt
     6181 ttgtatccca gcatgaagga agtgaaaaag gaatggctgg agtccgcaca attgctgggc
     6241 gcttcatcag cgtatgcttg gttcaaggtg gctttgcctt ttttgcgacc atcgattgcc
     6301 agcacctttg tcatcttatt tgctaatggc atggggacct atgaaacagc gtatgccctg
     6361 accagcagta atgtaaatct tttaacgatt cgaattgcgg ctttagtgtc gggagatgtg
     6421 ttcgccaagc ctaatttagg cagtgcgttg gcagtcgctt taggtgtcct gctggtaacg
     6481 gcaatgttga ttattcaaaa gaaaggcaag gtggtgcaga cgtgaaaaag ttaataaaac
     6541 cgattttgtt ctgctttgtc ctgtattact tgctgccgat tattggaacg attttgtatg
     6601 ccagctccac taaatggtca aaatcgcttt tacctagtga ttttaccttg caatggttcc
     6661 aacagctgct gacggacagg gaatttattg ctgcggtggg tcgctcactt ttattagcag
     6721 gtgtggtctt ggtgacgatt ctgttgttga tgattcccac cattatttgg attcatttgt
     6781 atttccctcg tttgaatcgt tggctggaaa agctgctcct gctgccgtat gctttaccag
     6841 gagtgatttt ggtaacggct ttgcttcgaa cgtatgctga aactggtatc ccaatgtttg
     6901 tagtgttggt aggtgcattg tttattacgg cgttgccgat tgtctatctt agtcttaaca
     6961 accagatgcg cttaattaat ctgaaggaat tagtggatgc agcagaaacc ttgggcgccc
     7021 cgatgtctac gattattatc caggtgctgt ttccaaacat ccgtatcggt gtgaccctag
     7081 tttctctgat gattttttct tcggtttttg gggagtacat gctgacgaac ctgttgattg
     7141 gcggtcgatt tcagacggta cgcatctata tgctgcggcg gatgaacgaa aacggccatt
     7201 tggctagcgc tgtgatggtt ttgtatttgc tgtttatggt gggaattgct ttaactacct
     7261 ttatgctgac ttatcgacaa aagcaacagc tgtcccttaa gcagacgagg gaagagaagc
     7321 agtcgaaaaa gaagccctta aaggagttgt gacggaaaat gtttttagaa atacatgatc
     7381 tcaatctgtt ttttgatgaa aaacagattt tagctattga tgaaattcag attgaaaagg
     7441 gtgagctggt tactttacta ggtcccagcg ggtgcggaaa gtctacgttg ctgcgctgta
     7501 tcacgggatt ggaacaacca aagagcggtg agattgtgct ggacaatgag aatattgccg
     7561 ataagcctac gaaggatcgc aatatcggct ttgtttttca acagtatgca ttgtttccaa
     7621 ctatgactgt ttttgaaaat gtagcctttg gtttgaaggt aaagaagctg agtcctgagg
     7681 tgattcagga aaaggttttt gagatgctat cattggtgga tatgacggag caggcagata
     7741 aaaatgtcca gtttctttct ggcggtcaac gacaacgggt ggcactggct cgctcattgg
     7801 tcactgaacc gaaagtcctg ctgctggatg agccattaag tgctttggat gcccgaatta
     7861 gaaaacagct gcagcgggat ttgcgcgcta tccagcaatc gctagggatg acgatgattt
     7921 ttgtgactca tgaccaagaa gaggcgatgc gaatcagtga tcggattttt gtgatggaag
     7981 ctgggcgggt ggctcaggtt tccacctcga aggaattgta tcagaatcct cagagccgat
     8041 ttgttgcgga atttatcggc aactataatc gtttcgaatg gtatgagctg aatcagtata
     8101 cccacgagtt ttccgctaag ggcaatcgct gcatttatta tttaagaccg gagctgattc
     8161 aatttgaacc ggttcctcag ggggttaaga ttccggttct atggaaggaa tcatttattt
     8221 taggcaacat tcaacggtat gtttttcaaa cgctggaggg caaagaaatt ttagtggatc
     8281 ggttgaacga tcgggaagag aaaattagcg atgccttata cattcagctg aaagatattc
     8341 tagcaatccc tgtggaaatt gatactcagc attttgcaca acaaagctag tgtatagtcg
     8401 gcaaacattt ctggctcttt gtcaaatagg actgatagag tgatataaga acatctaagg
     8461 tagaattgat tttctgcttt agatgttttt ttatgtttaa taagtgatca gatgattgat
     8521 taagaaaatt ctggttctca tcctaacccg taagctattc gtttcaatgc tttgatgtgg
     8581 gtatgcagat tttctatttt gccatttgag taaggcaact ctaaggctaa acgaatgccg
     8641 gattcatagt ttataaacgc tttttttata tgatgcagtt ctcttggaac cgacttgggc
     8701 attgttttta caagctgaaa gaagatgtcc gcttctttgt cataaaaagc ttgaagaatt
     8761 tcttgatagt aagcataagc tattcgtagt tcatcagaag tagaaagtag acgttctacg
     8821 acttcacttt cggttaaata ggcccaacca aaattacgcc attttgtcca tttatcataa
     8881 acaagcgtgt cctgtcgttt gatcagaagt cgagataaag ccttcacttg tcgatagtgt
     8941 ttggcttgta ttggatcgtt tcggtcgaag cttttcatga ctcatagtca aatatcctta
     9001 aaactgcgaa tgaggtgctt gacgatgtga aagcgatcca caacgagttt ggcatgagga
     9061 aagcactctt ttatcagcaa agcataagag gcgttcatat ctgttacgag tatttttacg
     9121 tttaaacgtg cacgtcgttc aaattgcata aaataagaaa tgagatcttt cttcttacgt
     9181 gaaggaagaa tatccagtaa tttcccagta tcgccatcca tacaagaaaa agaataggga
     9241 ccgacttgat ttttcaatga acgaaattca tctaccaatg aacagcgagg caaccaattt
     9301 attttcacgt ttgtcatggg gagcagagaa gcgagtgttc gcgaaacggt tgaccaagaa
     9361 acgccgcaat gtttagaaat cgtcgtcatt gcttgttttt cagtcaattc ctccaatatt
     9421 ttataatgta cttgtttggc gataaaacag tttttttcaa caatgtaggt attcgccgta
     9481 aaataggtgt gacagccacg acagaggtac cgttgctttg ccaaagcaag ataagtccgt
     9541 tgattcgcac acttattgag aaggaccagc gattttttct ttccgttctt aacgatcagc
     9601 ccatgttgat tcacctgttt acactgagga cagggctgaa tcgttttaat gagcgtggca
     9661 gaaacaacga tactgttgat tgaatgtatt tttttcttct cgacatgagt gatttgaatg
     9721 tttccatctt tgattcctag taatagtttg ataagatgaa gatcaggcat acagaacgct
     9781 cctttgattg tgttgtggtg attcaattct aacggagctt ctgtatgttt tgttttttta
     9841 tgctcaaaag gactgataga atgatttact atcagtccta gaaattatag cgccacattt
     9901 ctttcacctt tgacttttca gacggcagtt tttatgctga tactgtagag agagcaggac
     9961 acagcttttg cttgttttgg gtaaggggga aagaagatga ccaaagcact tatttttgat
    10021 tttgatggga cgatgattga cagtgaatgg atgttttacg agagtattca tcactattta
    10081 ctgaagccta tttactggag cagttcggct ataaaattcc ttttgcagac tatcaacaag
    10141 gtgttgggac ggcagcagaa ctgtcctttt atgaagggat aagtcaagcg gttgggagac
    10201 cagtggatgc tgaacatttt gagcagcgga tcagcagcac tggaaaatcg gagtagtctc
    10261 aattctgaac tgcgggagct agactatttt tttgaagcac atccgatgat taaggaaaaa
    10321 attgattcct ttgtgacgat cgatgatctt caggtcggca aaccagatcc agaaggttat
    10381 ttgttgtgct tgaaacggct gagggttgaa caggctgttg tagttgagga tagtccagtc
    10441 ggtgcccaag cagcaccgca tatgacagtt gatgtacaag cgttggatgc agattttttc
    10501 gcctttagcg ggcataaaat gtgcgggccg acaggaatcg gtgtattata tggcaaacgt
    10561 caattgttag aacaaatgga gccagtcgaa ttcggcggtg aaatgataga ttttgtcaat
    10621 ctttacgata gttcatggaa agagctgcca tggaaatttg aagctggtac tccgaacatt
    10681 gcaggagcaa tcgattactt gaatgagatt ggtatggaga atattcatcg ttatgagcaa
    10741 gaactggttg cctacgtttt acctaaatta caagcgatcg gcggtatcac catttacgga
    10801 ccgcaagatc ctgaacatca tactggcgtg gttgctttta atttggatgg catccatccc
    10861 cgtgatgtgg cgactgctct ggatatggaa ggtgtcgctg tacgttcagg acaccattgt
    10921 gcacaacggt tcatcgacga tttctgtttg actaattggt cgtttaaaca gtcgatgata
    10981 atgccgtttg agcggctcca atgtcgtgga atctttgagt aagagtttcc aatatcgctt
    11041 gagccgacgg tacttcttct gattttcaga ttacgtgatt gattttcgta cagttctatg
    11101 agaatcgcct gcttcagttc ctttgttagt tgatgatgtt catcaactaa gtccgttttc
    11161 gcagaaaagg tctgcccaca atcatagcat agaaaacggg tacgttttaa ttctaaaata
    11221 gtcaactttt ctttgacttt caacatctga gtacgtgtcg tataggaacc gttgcggata
    11281 atacgaatcg atccacagtg ttcacaatgg gaaggtgagt catccaatct ccctttaata
    11341 aactgcgctt gaacgccttt atattctttt tcaatcagcc attcttcatc aaaaaataaa
    11401 tgtttatctg ttaatccgag taattttcta gtatgattat ccatagagcg actcctttgg
    11461 tagggtgatg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt gagttgaaac
    11521 gaagaaaaag tgttagtaga aaatcgcctt cgattttcca acaacacaaa atattataga
    11581 agcctttttt ctaaaggatt tcaaggtgtc tgtttgcatt tattaaaaaa aagaaaaagt
    11641 attggcaaca gaaggcgtca ccaacactag atattataga gccataatat gctgtataat
    11701 atctagtgta taggcttaag tggaggtata tatgaaaata ttaactgttg aagatgataa
    11761 tatgataaga gagggaataa gtgaatatct ttcagaattt ggatatactg ttattcaagc
    11821 taaagatgga agagaagcct tgtcaaaatt taatagcgat ataaatttgg ttatcttaga
    11881 cattcagata ccttttataa atggtttaga agtgttgaaa gaaattagaa agaaaagcaa
    11941 tttaccaatt ctaatcttga ctgcatttag tgatgaagaa tataaaattg atgcatttac
    12001 taatttagta gacggatatg tagaaaagcc attttcattg ccggtcttaa aagctagaat
    12061 agattcttta attaagaaga attatggaca tttagagaag tttgaatata aggatctatc
    12121 ggttaatttt aatagctata cagctaaaat aaatggtgaa gaaatagatg taaatgcaaa
    12181 agaactagaa gtattaaaat gtttattgga taataatgga caagtgttaa caagaatgca
    12241 aattattgat tatgtatgga aagatagcga agaaactcct tatgatcgag taatagatgt
    12301 atatataaaa gaacttagaa aaaaattaca a
//
LOCUS       DAJSNV010000078        12246 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSNV010000078 DAJSNV010000000
VERSION     DAJSNV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.081"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON516_002098"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON516_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3345570.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON516_002099"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON516_002099"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3345571.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ON516_002100"
     CDS             complement(2169..2906)
                     /locus_tag="ON516_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3345572.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ON516_002101"
     CDS             complement(2997..3524)
                     /locus_tag="ON516_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345573.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ON516_002102"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ON516_002102"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3345574.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ON516_002103"
     CDS             complement(4401..4886)
                     /locus_tag="ON516_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3345575.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ON516_002104"
     CDS             complement(5039..5236)
                     /locus_tag="ON516_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345576.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON516_002105"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON516_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3345577.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON516_002106"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON516_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3345578.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ON516_002107"
     CDS             9211..9423
                     /locus_tag="ON516_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3345579.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ON516_002108"
     CDS             9425..11104
                     /locus_tag="ON516_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3345580.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ON516_002109"
     CDS             complement(11358..11558)
                     /locus_tag="ON516_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3345581.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="ON516_002110"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="ON516_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3575 a         2635 c         2117 g         3919 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaat
//
LOCUS       DAJSNV010000079        12163 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSNV010000079 DAJSNV010000000
VERSION     DAJSNV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.082"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            258..533
                     /locus_tag="ON516_002111"
     CDS             258..533
                     /locus_tag="ON516_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345582.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2618
                     /locus_tag="ON516_002112"
     CDS             546..2618
                     /locus_tag="ON516_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3345583.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2783..4669
                     /locus_tag="ON516_002113"
     CDS             2783..4669
                     /locus_tag="ON516_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3345584.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="ON516_002114"
     CDS             4681..5628
                     /locus_tag="ON516_002114"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3345585.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="ON516_002115"
     CDS             5648..6175
                     /locus_tag="ON516_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3345586.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="ON516_002116"
     CDS             complement(6238..6891)
                     /locus_tag="ON516_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3345587.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="ON516_002117"
     CDS             7023..7865
                     /locus_tag="ON516_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3345588.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="ON516_002118"
     CDS             8023..8919
                     /locus_tag="ON516_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3345589.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="ON516_002119"
     CDS             8922..9635
                     /locus_tag="ON516_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3345590.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="ON516_002120"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="ON516_002120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3345591.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="ON516_002121"
     CDS             10170..10397
                     /locus_tag="ON516_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3345592.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="ON516_002122"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="ON516_002122"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3345593.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="ON516_002123"
     CDS             complement(11183..11683)
                     /locus_tag="ON516_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3345594.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..>12163)
                     /locus_tag="ON516_002124"
     CDS             complement(11962..>12163)
                     /locus_tag="ON516_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3345595.1"
                     /translation="DNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEE
                     EMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4229 a         2072 c         2458 g         3404 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcgttttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tca
//
LOCUS       DAJSNV010000080        12024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSNV010000080 DAJSNV010000000
VERSION     DAJSNV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.083"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(49..2133)
                     /gene="cadA"
                     /locus_tag="ON516_002125"
     CDS             complement(49..2133)
                     /gene="cadA"
                     /locus_tag="ON516_002125"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3345596.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(2657..3016)
                     /locus_tag="ON516_002126"
     CDS             complement(2657..3016)
                     /locus_tag="ON516_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3345597.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3046..3387)
                     /locus_tag="ON516_002127"
     CDS             complement(3046..3387)
                     /locus_tag="ON516_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345598.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3384..4058)
                     /locus_tag="ON516_002128"
     CDS             complement(3384..4058)
                     /locus_tag="ON516_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3345599.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(4854..6152)
                     /gene="asnS"
                     /locus_tag="ON516_002129"
     CDS             complement(4854..6152)
                     /gene="asnS"
                     /locus_tag="ON516_002129"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3345600.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6192..7382)
                     /locus_tag="ON516_002130"
     CDS             complement(6192..7382)
                     /locus_tag="ON516_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3345601.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7403..7930)
                     /locus_tag="ON516_002131"
     CDS             complement(7403..7930)
                     /locus_tag="ON516_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3345602.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(7977..10766)
                     /locus_tag="ON516_002132"
     CDS             complement(7977..10766)
                     /locus_tag="ON516_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3345603.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(10913..11113)
                     /locus_tag="ON516_002133"
     CDS             complement(10913..11113)
                     /locus_tag="ON516_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3345604.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11565..11885)
                     /locus_tag="ON516_002134"
     CDS             complement(11565..11885)
                     /locus_tag="ON516_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345605.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         3323 a         2592 c         2108 g         4001 t
ORIGIN      
        1 agaaaaggat ataaaaaaaa gaggacgttc attcgtcctc ttcattgatc aacgtttatt
       61 ttttacatgc aagcttcgca tggcattcaa aacagctaat actgtcaccc caacatctgc
      121 aaatactgca tctcccattg atgcaaaacc aaaagcacct aaggaaagta ccaaaatttt
      181 tatcccaata gcaaagacaa tgttctgttt gacgattttc aatgttttct ttgctaaatg
      241 gattgcttct gcaatcttcg atggttgatc attcatgatg actacatctg ctgcttcaat
      301 tgcagcatcg cttcccaatc cacccattgc gattcctaca tctgctcttg ctagcaccgg
      361 tgcatcgttg attccatctc cgacaaatgc ggtcttttta ttgttacttt gtaagatttc
      421 ctctaaccgc tgcactttat cttctggaag caattcgcta tatatcttgt ccacgcctac
      481 ttgcttcccg atatgatcag caattttttt cgagtcgccc gtcaacatga ctgtgttttt
      541 cacaccagcc tgttttaatt gaaccaatgc gtctacggca tcttctttca aggtatctgc
      601 aataacaaga taaccactgt aactttgatc gatcgctaga taaagcaatg taccgatttc
      661 ttgacttgaa ggaaaagaga tttcaaatct ttccatcaat ttgtgattac ctactaatac
      721 ttgtttgcca tcaacttcag ctaagatccc ttcacctgca aattcctgga tattagctgt
      781 tggtaaaagc ggacctgcgt agccttctaa aacagattga gcaatcggat gtgtagaaaa
      841 ctgttctgca cttgcaacat attgcataaa tttctttgga tcaattcctg ttggatcgat
      901 cgtctggatc gaaaagtcac ctttcgttaa tgtgccagtt ttatcgaata ccactgtctc
      961 agtttgtgca agcaactcca agtaatttga accttttact aatacaccga ttttacttgc
     1021 tcctccgata ccgccaaaga aactaagtgg cacggaaatc actaatgcac atggacatga
     1081 gatcactaaa aatgtcaatg ctcgatagat ccattcagaa aacgccgctc tagaaacaac
     1141 aagtggaggt aaaacagcta ataaaacggc caatcctaca acgactggtg tgtaatagcg
     1201 cgcgaattta gtaatgaaat tttcagctgg tgctttttta ctgctggcat tttctactaa
     1261 ttctaataat ttgctgatag tagaattttc ataagatttt tctacttgaa ctaaaaatgg
     1321 tttattttta ttgataaatc cgcttaatac tgtttcacca acatatacag atttagggac
     1381 tgattctcct gtaagtgctg acgtatcgac taatgattct ccttcgatga tcgttccatc
     1441 taacggtaca cgttcaccag gcttcacaag tacaaactga ccgatcagca cgtcttcagg
     1501 gttcacggtc atcaatcctt ccgccgtctg cacgtgagct gtttctggac ggatcgccat
     1561 caattcgcga atcgattttc ttgaatgact gactgccaat ccttggaaat actcgccgat
     1621 ttgataaaat agcatcactg ctacggcttc tggatattca ccgatcaaca tagcgccaat
     1681 cgtagcaaca gacataagaa aattctcatc aaagattttt cctttgacga tattcatcac
     1741 tgccttcttc actacatcat atcctatcag cagataaatc aataaatacc ctatcaagcg
     1801 aattggttca ttaaaatcaa ataatagcaa taaagcgata cctgcaaggg aaaataaaat
     1861 ccggaacagc gtatttctct ttttttcatt tccatgttca gatacttttt ccttatctat
     1921 ttctgttact ttgacatctg gttctaattc cttcactact ttttttgttt cttgttcaat
     1981 cgtagccaaa tgatcttcct ctgcgtcaat cgttagtttt ccagaagtga agtttaccgt
     2041 tgcttccttg actccattga ttttctgaac tcctttttcg attttcattg cacagttcgc
     2101 acaatctaaa ccttctaatc tgtaactttt cattttaatg acctcctcgc ctcaacatat
     2161 gaatatctct tcataactaa ttataaatga atggttattc atatgtcaag gtatccgaaa
     2221 gaaaaaatga aaaacatctg catcggaaaa tttcttccat acagatgttt tggcttattc
     2281 tttctttttc tcttcgatca ttgccactgc tcttatccaa cccgcacttg ccttcttgat
     2341 cttgacagga tagcaactaa gtttgaagcc tgttgctggc aatttgtcta aattcattaa
     2401 tttttcgata ggacaatatc ttttttcaat ccctgcaaaa tgtccttccc agatatacga
     2461 agctccacta cgctttcaaa aatatagaaa gagttcttta atgaaaatta tttcaacttt
     2521 ccataaacag taaccccata taaataaaag acgtaaaagt cacatagaag aacttataca
     2581 ggaaaacgca tagagtcagg tgaaatacat gtatcccacc tggctctatg ctttatctaa
     2641 cctttattat tctcttttat acaatttctg caccgaatgg catcaacgcc aatcgagcaa
     2701 gtttgaaaga ttgcttggca aaaggtatcc ctatgatcgt gatacataga aggatgccgc
     2761 tgacgacatg tgcgagagca attggtaggc ctgaaacgat cagccagatg atattaacga
     2821 tcaatgacat gccagactgg tgataaatta cttctttgcc aaaaggcgca aaagaaaggg
     2881 aggcgatctt aaaacattgc ataccgattg ggatacctat gatcgtgata caccaaagta
     2941 tccccgctgc taaccagcta agaccaccga ttagtccgcc gaatataaac cagatgacat
     3001 ttcctaaaca actcattgta tttctccttc tattactgtt tttgattaaa actgggcaaa
     3061 aattgatttt attcttcttt ttttagcttc cagcatcaag tattctgcaa attttttttg
     3121 gatttcttcc gtattttctt ttgttactaa tgtgtgttgg attagttttc ctgatggtcg
     3181 ctgatccaat cgttcttctg actgttgcac gtccgcttgc gcaaatccga taatccgttt
     3241 ttcttttgtt acagcaacga gcaatagttt attcttatca tttaggtcag ctgccgtttt
     3301 tgcttttacg aatgattctg gatgatcagt aatcagctga ttgatttgag gtaaatcttc
     3361 ttttactgcc caccttattt ccatcatttt tcacttttct ttcgctcatt tttatctcca
     3421 ctaaaaaaat cgactgcgac aattgcatct atccagcttt gtgcaatgat ctcatgtccg
     3481 ccaatcgttg ggtggacccc atcttctctt gtatatttcg aaaaaccata agcaatccct
     3541 aaagcattga atataccatc taaaggaacc agcaatgcgc catattcctt agcaagttta
     3601 cgaacaattt ggattttagg gtctaagtct ttacgccagc catgacggtc gtcggttata
     3661 ggaagtacaa acggctccat caagatgatg ttgcagattt ccgctgtttg aagttcattt
     3721 agtaattttc gataaacgga ttcaaattgc tgagcagatt tctcagttcc gaatatagcc
     3781 tgatcgctag tattatgcca ggtatcattg attccgatca aaatcgatac atagtcaggt
     3841 tgtaaagcta aacaatcttc ttgccagcgt acttgcagat ctgcaatttt gtctcctcct
     3901 atcccacggt tcatgcattc aaattccatc gttggatatt gggagattag ttttccggca
     3961 atcaaaagtg gataaccttt acctagatca gttgcttctg ctcggtatct tcctgcgtct
     4021 gtaatgctat caccgataaa taatatacgt tttttcattt gtcaaaaact cctttttgaa
     4081 taaaacgatg attttcttcc ttatattatc ccataagccc tatcaagacg aaagagaaaa
     4141 tatatgtata aaaaatgaca ccagcctagt tccttcaaac aattgaatga acgctcatca
     4201 agtgtctagt tttttatgct atttttttct taacgtgccg gattcccctt gattccttcg
     4261 ttgtttggtc cagagagatc gatcacaccc aagcttccgt caatcagacc actgatgacg
     4321 ccatcgaaac atatatttat aggctcatct aagacttcta gtaaatgtcc ctttactaat
     4381 atctcttcta gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa
     4441 gggtgaattg aacttacata tggattattg catgctgaaa atacggacct actccatctt
     4501 cgaatgttac tattagtttg ttttcaggtg ctagatgctg ctttatttca acagcagaag
     4561 catcatcaaa tcagatttcc atttgaatta ccttctttgt aaatgtttga ttgattgcga
     4621 tttcgttata gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc
     4681 tggaaacttc cattatcccg tatttaaaaa aaaagaactg cgagagttcg cagttctttt
     4741 ttttaaataa acgttgctct ccggccacaa gtcacaatag tcatcaaaca tgaaaaactg
     4801 ttttttgaaa ttactccttg tgactcggag ctgaacggca ggcgcacaac ctcttatgga
     4861 taaattcggt tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca
     4921 atccaagtga ctgttcgttc aagacctaga ccaaaaccag agtgcggaac agaaccgtat
     4981 ttacgtagat caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt
     5041 tctagcaaga aatcatagtc cgttgcccgt tcgcttccgc caataatttc accgtaacct
     5101 tctggtgcga tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg
     5161 tagaatggct tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc
     5221 gcaatgaacg tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca
     5281 tttttcttca gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta
     5341 taagttttta agacttcttt gtctcgtcct agtacatcta atgcgtattc gcaatgatct
     5401 aaaacatttt gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga
     5461 tgagtgaaag ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt
     5521 gatttttcag cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca
     5581 gcttccatgt ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt
     5641 tcgaatagat ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg
     5701 acaaaatgat gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc
     5761 ataatcgcat gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct
     5821 gttccgtgtt cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt
     5881 gtaattccga tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact
     5941 aacacggatg tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg
     6001 ctttttacaa ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgtgatt
     6061 tttccgcttg atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg
     6121 tttttcgaat cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa
     6181 tttcttcagg attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct
     6241 tcttctaacg tagcgcgatc agtagcataa ctcatgcgga cattttctgg tgccccaaag
     6301 ccttcgccag tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg
     6361 acattttcat atttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct
     6421 ccttgcggct tttcaagaga aacacctggc agttctgcca caagcggata taacgtattc
     6481 aggcgttctt caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct
     6541 tcaacagctg catattgact gactgctgtc ggattactgg ttgactggga agcaacagca
     6601 atcattccat tgatgatctc agaattcccc accgcataac caatgcgcca gcctgtcatc
     6661 gcatatgttt tagacacgcc attgatgatg atggtctgat ttttgattgc ttctgaaatc
     6721 gttgcaattg gagtaaactc attgccatta taaactaaac gaccataaat atcatcagca
     6781 actatcaaaa tatcattttt tactgcccat tcaccgattg cctgaagttc attcttgcta
     6841 taaatcattc cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa
     6901 cgtgcttgtt ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca
     6961 aagacaggaa gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca
     7021 ggaatgatga cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat
     7081 ttcgctccgt ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc
     7141 agataatcaa cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta
     7201 gctttcccat tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa
     7261 tccggttctc ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc
     7321 tttgccgcag ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc
     7381 atcccattca ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt
     7441 caaagccaat caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca
     7501 caggctgatc atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg
     7561 cagcaattgt ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta
     7621 ctttttcacc agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg
     7681 tcagtgaaaa gtatgtcttc tttcgagtaa accaataaaa atgatcggct tcctcaagat
     7741 ttgcatattt ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg
     7801 aacgcaagta aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa
     7861 gtaacacgcg attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct
     7921 ttatcttcat tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa
     7981 cttccttctt catttttctt taagaattca tggatgtcct gatagatttc ctccatagct
     8041 agctctttga tcggtacttt tttcggtaat gcatggatga tccgtgcacc atattttgca
     8101 gtgactagtc ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag
     8161 cgacctaatg cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga
     8221 ttgatcccct ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta
     8281 tcgaagggta aacgtgtgac tacaacgatc tgcaaagcat ccccaggcaa atctacacct
     8341 tcccaaaagc tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa
     8401 agcttctccc ggcttccgcc aaacccttga gctagtattt ctcgtccttt ttctagcata
     8461 gacagatgca ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt
     8521 attggccgat cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat
     8581 gcttgattgc tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt
     8641 ttctcataat cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagccgc
     8701 ttagagagat agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga
     8761 tcatatcgag gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga
     8821 aaaatcatct gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag
     8881 tcttccagcc attgatccat cagcaagtac tgcttgtcta attgatcaaa taagctatat
     8941 aaacttgcta aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc
     9001 agacgcattt gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg
     9061 gctgtcccag attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct
     9121 ggatactgtc cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa
     9181 atctgtgctt cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct
     9241 tcatgttctt tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat
     9301 tgctggattt gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg
     9361 atttccggca aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt
     9421 tcttttcgct gtgtttcttg tattaaaaac gcatgattga caatcaaaaa attactttgc
     9481 ctgattttct tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct
     9541 aaaaatccaa tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa
     9601 tgtaattcat ccaaatcacc ggtcttcgtt tgcgtcagcc aaactaagac cgccatttga
     9661 tacaaagcat attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgtaaa
     9721 tcaatatagt ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga
     9781 ttcagcaatg gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg
     9841 atgacagcgg gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt
     9901 ttacccattc ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg
     9961 tcgtagaccg cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac
    10021 attttttctt ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg
    10081 aatagttcca cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca
    10141 agtggctctg gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc
    10201 atacctgtgt gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc
    10261 atccgttctt cgatcagcaa aagaagttga cccgtcactt cggcatcact atctgcctga
    10321 tgaggattct catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt
    10381 tccgtcggca agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta
    10441 ggaagtccgc atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc
    10501 gcgacaaata tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa
    10561 ggcgcttttt ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc
    10621 ttccgccctg gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg
    10681 acgcagccaa attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca
    10741 acgactgcat aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt
    10801 ttgaaattat actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca
    10861 aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt
    10921 tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt
    10981 ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat
    11041 cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg
    11101 tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga
    11161 atggtgtata tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa
    11221 ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat
    11281 taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt taaaataaac
    11341 gacatattgt tcagttggtg tttttttgct ctgatgttct tctttaaact aaaaaaatta
    11401 ttctttctga ctacttatcg gttctgtttc aacggtcgag gctggggaag attctttagt
    11461 aggtctgatg ttctcatggt atttctgata gcctatgaca gttaccagaa gaacaaggca
    11521 aaccaatacg atcaacgaga ttagtttttc ttttttgctc atatctatac ctcataaata
    11581 acaggatgat gtccatcata tttctgagta tgagtaaaag caaaagctgc agctgtagaa
    11641 tgtaagaaat gccagcaact ttcataatca caggtcggac agtaccaaaa gtaaccaaat
    11701 cttggctgca aagaccgttc ttctgtttca ggaatcgcag aatcaagatc tgttgtctgt
    11761 acataaacat ttttgtacag acgtgtgcct gtaataggag accgctgtgt ttcttcagca
    11821 aaaactggct gacttgttaa acttagggat agaaacagca aacaccataa cagtcttttt
    11881 ttcataaaga cctccttatc gataagtttt tcccaattaa aatcatatct caagaacgcc
    11941 cgttattcac attttgttca taaatgatgt tttttgtatc ataatcgtca aaaagaggtc
    12001 gtgaccacgt cacaacctct tttt
//
LOCUS       DAJSNV010000081        11789 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSNV010000081 DAJSNV010000000
VERSION     DAJSNV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.084"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            36..458
                     /locus_tag="ON516_002135"
     CDS             36..458
                     /locus_tag="ON516_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345606.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            477..2069
                     /locus_tag="ON516_002136"
     CDS             477..2069
                     /locus_tag="ON516_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345607.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2081..2416
                     /locus_tag="ON516_002137"
     CDS             2081..2416
                     /locus_tag="ON516_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345608.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2563..2757
                     /locus_tag="ON516_002138"
     CDS             2563..2757
                     /locus_tag="ON516_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345609.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2789..5161
                     /locus_tag="ON516_002139"
     CDS             2789..5161
                     /locus_tag="ON516_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345610.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5222..6682
                     /locus_tag="ON516_002140"
     CDS             5222..6682
                     /locus_tag="ON516_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3345611.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            6693..8897
                     /gene="topB"
                     /locus_tag="ON516_002141"
     CDS             6693..8897
                     /gene="topB"
                     /locus_tag="ON516_002141"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3345612.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            8913..9062
                     /locus_tag="ON516_002142"
     CDS             8913..9062
                     /locus_tag="ON516_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345613.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            9136..9729
                     /locus_tag="ON516_002143"
     CDS             9136..9729
                     /locus_tag="ON516_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3345614.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            9745..10644
                     /locus_tag="ON516_002144"
     CDS             9745..10644
                     /locus_tag="ON516_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3345615.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            10647..11132
                     /gene="ssb"
                     /locus_tag="ON516_002145"
     CDS             10647..11132
                     /gene="ssb"
                     /locus_tag="ON516_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3345616.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            11498..11758
                     /locus_tag="ON516_002146"
     CDS             11498..11758
                     /locus_tag="ON516_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345617.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         4516 a         1929 c         2433 g         2911 t
ORIGIN      
        1 tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca agaaaacatg
       61 tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact catgaagata
      121 tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag
      181 aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt tctattttaa
      241 ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata ctaattggta
      301 caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt aaatccaatc
      361 aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt cataccagag
      421 tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag aacgtgatga
      481 ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa aaatcttttt
      541 caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa cgacgtacgc
      601 ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg
      661 aaggagaaac tttttcggaa agtaagacgg ataaagaatt cagtgaaacg tcgaatgaag
      721 ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt agaggaacaa gattttacca
      781 aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa
      841 gggaacaaaa agaataccaa cggttgaacc aagcaatgaa aaaatatcaa acagagccac
      901 ataaaaaagc ttcaggaaat cttcctggag ttttttctat ggcttcagac gaagtgaaag
      961 gaagtgatct gaaggagatc aaacaaatct tccaaagagg acaacaaaaa ggctcaatca
     1021 tttatcaaga tgttatctct cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc
     1081 ccaaaacaga taccttagat gaagaaggtt tgaaagcagc tggaagaaaa atgatggaga
     1141 cattattcga gaaagaacaa ttgaatgaaa caggttactg gatggcaacg attcaccgta
     1201 acactaagca tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaaccgt
     1261 atactgagat tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga agacaagcaa
     1321 cattagacgc aatgaaaacc aattatgtac atgagttaga acgtttctct tatgaaaagg
     1381 atcgaatgat cggtatggag aaacgaaatt tattgacacg aaaaagtgat ttgcgtaaca
     1441 ccttaacaaa agtagtaaaa gaaccagcac gttacgatcg acacgccatg aacttattaa
     1501 atgaagtata taaaacccta cctgaaaaac gttcggaatg gaattatgga gatgaaaatc
     1561 gaaccaagct ttcaccattt acaaggggaa aacttgatga gttaaccaaa tatgttttaa
     1621 caaaagaacc tggatatcaa gaatatatac agatttcaaa agcattgaaa gaagaagcca
     1681 aaagccttta tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac gccctctttg
     1741 atttaaaaaa gcgcacagga aatgcgattt taacagaact gaagagacag gatagtcaaa
     1801 taaaaccatt aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa cgatcctttc
     1861 gaacactcaa acagttgcca acaaatgaat acaagtatag ttatcaagaa ttcgtaaagc
     1921 gtaggaaaca attccaacaa agagttgtca cgcagcgaag cttgcgtcaa ttaaatcgaa
     1981 caattaatgc acatgaagaa aaataccacg caagtaaagc atatgaagag caacagcgaa
     2041 gaatccaaat ggaacaagaa agacaatagg aggaagagaa atgaaacaat atttagatca
     2101 atggaaagtc atagaaggat cactaaaaga agaacgaatc gaacaacttc ctgattgttt
     2161 agaaaaagag catttgtttc agataagcga aatgttgaga aacgaacagt ttgatccaaa
     2221 tcaattttta gttgttgagt acccagctac tggtgtgtat tgttgtaatc atgtgaaggg
     2281 tgaaaaatat tttattattc aagaatacga aggtaagtta gcgccttatt atactacttg
     2341 ggaaataaat gaagagggaa tcaataattt cccatgtgaa agcattgaag agtctatttc
     2401 tctcacggaa tgttaaataa atagagacgt aaaagagtcc tgaacgtgat tgtccaggac
     2461 tctttttcaa tgaattgttt tccgccaatt cactgagtgg tgcgattcaa ttttattata
     2521 tcaaaagaaa gtgagattcc aaagtgaagg gaggaagaag aaatgactca gacaacgatt
     2581 ggtgttgtgg ttgaaaaaag cagggataat ctcgtttttg tgacagaaat tcaaacaggt
     2641 agagcttttg tagtcacaga caaagctgca aaagcgtatc aaaatggcaa tattttaacg
     2701 ttaaatctga ctacgaagat gttcgttgat gcagtagaaa attatccatt tgtttagaca
     2761 aacgatattt tacaaggagg aaacggacat ggcagaagaa aaagaaccac tagataatag
     2821 tcggttggga aagtccaaaa gaaaattagt acggttgcaa aacgaattaa ataatcaatt
     2881 tcaaaagcta tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg acaaacgaaa
     2941 tggtcgttca tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa gtttgagaga
     3001 agagatcaag caacaagaaa aacaggtaga aaaacttgaa agacaagaag agctaaagga
     3061 aatgggctat aacaagtacg gcggactgga tatgacgatc gataatattc caagaatcaa
     3121 agaagaaatc gaacgctttg aaaaaggtga atcgactttt tcagccgcca cgatccgaaa
     3181 atatcaacgg aagttggaaa cattagaaca gctgaaagaa aaatcagaaa aaggaaaaga
     3241 aaatatcctc cctgaagtac aagcgatcat tgacagcgga cgagtgacgc aatggaaaaa
     3301 aaatccaacc atttattttt tgaaaggcta tcgaaaagtt gctttagaac tgaatgaaaa
     3361 cgggggattt gaggaatcaa aaaaatacca agccacgtcg gaagaggaaa aagcagctgt
     3421 tcgtgaatta cttgaagagg ctgcaccaac tgattctctg aaggagaaag aaaaagatca
     3481 tttctatcga gtagaattca acgaaaatga tccagaaaaa ggattgaaaa gttatggtgg
     3541 ggagatcgta acattagaat tgatcgatca acttaaaacg ttggataacc aatacgctgc
     3601 agaagcaggt tattataaat tctattttga agaagtgaag aacggagaag tagtagatca
     3661 gatccgaatg gatctagggg atggcgtcaa cagtaatcac cccatctatt tagagttagc
     3721 aaaattgtgc gaacaagtac ctgagaagga acaaaaacaa tcggtcaaaa atgaacctgt
     3781 ttcgaaagaa caaatgcagt cgaaagaaac aaaaacaaaa caaaagggct atcaaaaacg
     3841 aacggcagaa gagatcaaac aagaagttaa atccctttcg caacaggcac tggatgcggt
     3901 gaaaaagcat acccattctt ctgaagacgt caaagaactt ttgaacttta tgtctcgttt
     3961 tccagaacga tcctttagaa atcaattatt gattgaggaa caatttccag gtgcaacggc
     4021 ttgtttaggt cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag gggaacaagg
     4081 gaacaagatc tttgtcagaa aaacggtcaa aggattttat gagaaaggac gtggctttgt
     4141 tcgagaaaca gaagctacac cagaagataa aaaacgaatc gaaagtggtc aaatcgaagt
     4201 gatcaaaaaa ccttactaca cgattgaaaa agtgtttgat atcacacaaa cacagttaaa
     4261 acctgaagac tatccgaaaa tctttccgaa ccgtgtcttt gattttcaat tggataaaaa
     4321 aggacagtca gaacttcaag ctgggattca agcggtagcc aacaatattg gaatacaaat
     4381 tcgagatatg acagaaagtg aagtctatca aagagaatta ggacaagcac gtggggcgta
     4441 tgttcgtaac gaatttactg gcgaagaaga aatcgtcatg aatactcgaa atactcctac
     4501 gcagcattta gctacaagta ttcatgaatt agcccatgca agaatgcaca aattttcaga
     4561 attagatacc gcaactaaag aattgcaggc agaaatgact tcttatattg tgtgcaaaca
     4621 ttttggaatg gacacgtcgg agaaagctat tccttatatt gcagcatgga cgaagaatgg
     4681 tcagacgttg gatgataagg aagcggcgga acgtgggaaa atcatgaatg atgtcagccg
     4741 tgtcgcaaat gaattcattc aaaccatttc taatgaaatc aatcaataca gagaaagaga
     4801 accagagatc caaccagcag aagtgaagca ggagcataaa aatactgtag aaacggcaga
     4861 gaataagaat attgtgcccg ttggttcact ttggatcgac aagaaagacg ggaaagtaat
     4921 ggaagaagat acgggtcgaa cgttacattt gaaggatgcg gcgtttattg aagatccgca
     4981 gcataatcaa gtgattttaa aaggaaaaag ttatggaaaa gagattcaaa cggcgttacc
     5041 ttcagaagaa tttgcgctag gaaatccatt ccgattttca cttgaaacag tgtcagaagt
     5101 ggccaaacga aaaaatgaaa tctattatga acaacaattg tcacaaaaag aacgcgtata
     5161 aaacaagtaa aaaagactat ctgaaaaacg gatagtcttt tttcttttgg gaggtttgaa
     5221 aatgaccgaa aaaaaaatga gtcttattga tcgatgtaaa cagatcgata tagtggattt
     5281 tgctcgaaac aatggaatgg ctgtagtcaa taaaggacga gactatcgct tagaagacca
     5341 tgattcattt gtttttgatc gaagaaaaca acgtttttat tggaacagtc aaaatattag
     5401 tggcgacatt attgaattag ctaaactttt tttcatagac aaagagatcc aagactctaa
     5461 gcaacaattt aaagcagcca ctgattttat tctaaagaat gaagacaaaa cagaacgagt
     5521 ggaaaatctt cattttgaaa cagaaaagta caaagatcac cccgttgatt atcaaccact
     5581 cacagaaaaa ggaaggaatt atctgaagga agaacgaaaa ctaccagagt ggctgattga
     5641 ttatgcagaa aaagaagggt tgattgccga actaaagcca aaatatgaac ggcaaaattt
     5701 cttagttcga gacgatcgtt tagatcatgc ggtcgccttt ttatggaaag atccacagac
     5761 aaaagagaca gtaggggcga gttatcaagg aacgttcatt gattatgaac gttttggtga
     5821 gagaggcaca tataagcata tagataaaaa ttcgacagcg aatcatggct ttaacctaaa
     5881 gatcggtgac cctaagcagt taaaattttt tgagagtagt attgatttac ttagctatgc
     5941 tgcactgaat cgtgatcagt taaacgatac gtggttagtc tctatggaag ggttaaaaca
     6001 ccatgtgatc agtcattatt ttggtgaggc agtatctgaa ttgagaaaga aacaagcctt
     6061 tcctcaatcc atcgagattt gtgtggataa tgaccgtgca ggacatattt tctacgaaaa
     6121 agaacaatta atgggcgcag tcgatccatt tacgaatcag aaagtacgtt gtgaacgagg
     6181 aatcgctaat gattggcaag tgccaaaaga atacaaaatc atttatgaag aagtcgcaaa
     6241 agaagaaaag gtcacaccag aagctattat ggccatacac aagacagaaa acaatctgca
     6301 attaacgaac caactggttt ccgctcacaa agtaaatgcg ttttttggcc agcagctttc
     6361 tgttaacgat tcaatagaag ccattaattt gaaagatatt tgtagagaag tcgcaaaaga
     6421 attaaaagtt tgtgaacgcg tggatggtac gtacgatttt gatcgttttt atcaagaaaa
     6481 aggggatatt aatgcgcaga ttcttttctc ttataaagca gaacaatact acaaaggcta
     6541 taaaaatcat gaacatgaat ttatacctga agtaaaaaag gactggaacg atcaattgaa
     6601 gcatgagatc cagcaacaag aaatcagaaa gcaaaaacgt gccatgttgt ttcaacaagg
     6661 cagacaacaa gaaagggagt gaaatcgtca tgatgaaaac cgttatttta gcagaaaagc
     6721 cgtcacaagc gaaagcttat gccgatagct tctctaaagc cactcgaaaa gacgggtatt
     6781 ttgaaatcca agatcgattg tttcctggtg aaactgtgat cacgtatggt tttggtcatt
     6841 tagtcgaatt agattcgccc gatatgtacg atgaaaattg gaaacaatgg tcattagaac
     6901 atttgcctat tttccctaca cattatcact atcatgttcc taaagataag aaaaagcaat
     6961 tcaaggtggt gaaacagcaa cttcaatcgg ccgacaccat tattattgcg acagatagcg
     7021 atcgtgaagg ggaactgatc gcttggacga tcattcaaca agtaggcgcc gatcaaggaa
     7081 aaacctttaa aagactttgg atcaactcct tagaaaaaga agccatttat cagggattcc
     7141 agcagttacg agacgcaaaa gagacctatc caaaatttga agaagcacaa gcacgtcaga
     7201 tcgccgattg gttaattggg atgaacggta gcccactgta tagtttactc ttgcagcaaa
     7261 aaggcatacc aggaagcttt tccttaggca gagtccaaac cccaacgctt tacatgattt
     7321 atcaattgca ggaaaagatt agaaatttca agaaagaacc ttattttgaa ggaaaatccg
     7381 aagtaatcgc tcaaaatggc gcatttgatg cgaagcttga tccgaatgaa acccaagcga
     7441 cgcaagaagc atttgaagat tatttaaaag aaaagggggt tcaattggga aaacagccag
     7501 gcatgattcg ccaagttgaa accgagaaaa aaagcgcggc aagtccacgt cttttttctc
     7561 tatccagcct tcagtctaaa atgaatcagc tcatgaaagc tagtgccaaa gacactttgg
     7621 aagccatgca aggcttgtat gaagggaaat atctaagcta tccacgaaca gacacccctt
     7681 atattacgga aggggagtat gcgtatttgt tggatcattt agatgaatac aagcattttt
     7741 taaaggccga atcaatccca acccctattc atacgccgaa tagtcggtat gtgaataaca
     7801 agaaagtcca ggaacattac gcgatcattc ctacaaaaac ggttatgaca gcagcggctt
     7861 ttgaacaact aagtccactg cagcaggcca tttatgaaca agtgttaaaa acaacggtgg
     7921 ccatgtttgc ggaaaaatat acctatgaag aaacaacgat cttgacgcaa gtccagcagc
     7981 ttcagttaaa ggcaatcgga aaagtaccga tagatctagg ttggaagaag ttatttggca
     8041 aagagaacga cggaaaagag aaggaagaag agccgctact tcctaaagtg acaaaaggag
     8101 aaatggtgac ggtagatcta caagtccttg aaaaagaaac caagccacca cagccttata
     8161 ccgaaggtac gttgatcacg gcgatgaaaa cagctgggaa aaccgttgat agtgaagaag
     8221 cgcaatccat attgaaagaa gtggaaggaa tcggtacaga agcgacacgg gcgaatatta
     8281 ttgaaacctt gaaacaaaag gaatatatca aggtagagaa aaataagctc gtggtgacga
     8341 ataaagggat tttgctttgc caagccgtgg aaaaagagcc gctgcttacg agtgccgaaa
     8401 tgaccgccaa atgggaaagc tacttgttaa agatcggtga acgaaaagga acacaaacca
     8461 cttttctagc caatatccaa aaatttgtct ctcacttatt ggaagtcgta ccagggcaaa
     8521 tccaatccac tgattttgga tcgacgttac aagaagtgaa agcagccagt gaaaagcaag
     8581 aagccgcgcg tcatttagga atttgtccaa aatgccagga acaagaagtc ttactctatc
     8641 aaaaggtggc agcatgtacg tctgaagcct gtgatttcaa actatggaca acgattgcca
     8701 aaaagaaact tacagcgaca cagctgaaag agatcattca aaatagccga acaagccaac
     8761 cagtgaaagg gttaaaaggt cagaagggtt cgtttgaagc aacgattgtt ttaaaagaag
     8821 acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac
     8881 gacgcactac aaaataatgg agggttaatc ttatgcgtaa tacaacacac aaacaacaag
     8941 aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac
     9001 gaagactgca gcaattgaag aaccagcagc ggattcaaaa agaaatcgaa ttagatcgat
     9061 aaattccttt cttgttaata taaaactaac atgttatagt tgtgttagtt ttatattaac
     9121 tttaggggaa gagtaatgaa agacaaaatc caatgtattt ttgataaaca taatggcaac
     9181 ctatccttga aggatgctag aagtgaagga ttatcgccta tgacgttaga tcgattagcc
     9241 gaaaaggata aaatagaaaa ggtagcacca ggtttctata ccttaaagga tgcgttcgtg
     9301 gatgaattgt atctcgctca gagtatctat tccagaggga ttttttctca tgagactgcg
     9361 ctcgatcttt ttcaagtagg aacatatatc cctaaaaaga tcaatatgat gtttcctaaa
     9421 ggatacaatg tatcaaagga gaaattagat cagtatgcca ttcaaccgca ttacactaat
     9481 aaagaaaatt ttgacctggg agtcacagaa acagaaagct tttacggcaa taaaatttta
     9541 gtctatgatt tagagcgaac gttatgtgac atgtggaatc caagatacaa agcaagcgta
     9601 gaggtaaaac aagaagcatt aaaagaatat atgacaagta ccagtagaaa tacaaataag
     9661 ctaagaagat acatggatat tttaaaatca ccaaaagaaa tgactttgta tatgttgcca
     9721 ctttattgag aggagtaaag atgaatgaca ccgacacaat ttaaagagaa aacaagaaat
     9781 ttcgcagcag agaaacattt aaacccacaa attgtccaaa gatattatat gatggaaaaa
     9841 ttattagaat gtattgttga atcgccttat catgatgatt ttgtattaaa agggggattt
     9901 ctgattggat caaaatacgg gttagaaaac cgttcaaccg ttgatattga tacgacttat
     9961 cgaaattcga aactaacaaa agaaaagtta gaaactgttt tttctgaatt aacagctaat
    10021 cctacaaaag atggaattcg ttttgaatta aagggattaa cagagacgag ggaagccgat
    10081 tattatccag gttttcaagc aaagtttatt gcttatcttg aaaatgcacg tgtacctttt
    10141 aaattagata ttacttcagg tgattctatt attccagcag cattgatgta tgaacacaaa
    10201 ttaatgtttg aagataaaac gattcatatt ccagcttatc ctacggaaca aattctagct
    10261 gaaaaattac atgccacgtt tagttttgga aaagataatt cgcgcatgaa agatttttat
    10321 gacctgtata tgattccgaa attagaacgt atcgatgaga aaaatttgta taagagtgtt
    10381 caaggaacat tcaaggaaag aaataatcaa tcggtgttta agcatttgta tgatcaggaa
    10441 atgcctcaat tggtgaaaga tgagggcttg agagaaaagt ggaaaagata ccaacaagaa
    10501 aactattttg ccaaagacat ttctttcgaa tctacactaa cttccgttga ttccctgatg
    10561 caatcaattg tagagcaaga gaaggcagaa cgaaaacaaa attttaagag atcacaacaa
    10621 atgaatcaag aaatggagag ataaagatga tcaataatgt aactttagta gggcgattaa
    10681 ctaaagatcc agatttacgt tataccgcaa gtggcacagc agtggcgact ttttccctag
    10741 ccgttaatcg taatttcacg aatcaaaacg gcaatcgaga agctgatttt atcaattgcg
    10801 tgatttggcg caaaccagca gaaacgatgg cgacattagc taaaaaaggc agcttgatcg
    10861 gcgttgtcgg acgaattcaa acacggactt atgacaacca gcaaggacaa cgtgtgtatg
    10921 tgacagaagt tgtggccgat aacttccaac tattagaaag taaagcagcg acagaaagcc
    10981 gtatgcacgc tgatcaatcg tccagcagtc caagccctac cacctttgaa caacgggata
    11041 cagcgacgcc taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa
    11101 tcgatattag cgatgatgat ctaccgttct aagtagcatt ttctattggt tgcatttgct
    11161 acggtttatg ttataattta gacaacaaaa agaattttca atctataacc aaaaaccact
    11221 agctggcggg ctagtggctt tcttttacgt ttctattata agaattaacg ggtaggctta
    11281 ctgtctttct tggcaaattt acaatcaagc catttgcaga tatagtgcgc ggctacttct
    11341 gccatgattg caaaaacaaa atcaaaaaga attttcaaac ctataacctc ctccccttgc
    11401 cagattaggc cggggtagca gacagcaaga ctattatagc ataattgaaa tatccaaatg
    11461 attggatttt tgctataatt ttagcgaggt gattgtgatg aacaaatatg ttggtttgct
    11521 tgataaaatc cgagtaataa aaacgcaacc gttattggtt cgctttactt tacaaacgat
    11581 tcatgaatcg attaattgtg tagttgcaga tattgaaatt atcgataaat tgctaattat
    11641 ggatgatgga aaatacaata ttgccgtcac aggtcacttc aataaacgaa atcagctagt
    11701 tattgctagc atgtatgtta gaaatcccga tcattttacg cgatcgatgg gcatataaat
    11761 aaaagaagag ttgtagtcct aaatgagga
//
LOCUS       DAJSNV010000082        11735 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSNV010000082 DAJSNV010000000
VERSION     DAJSNV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.085"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(368..1486)
                     /locus_tag="ON516_002147"
     CDS             complement(368..1486)
                     /locus_tag="ON516_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345618.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     NVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="ON516_002148"
     CDS             1957..3264
                     /locus_tag="ON516_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3345619.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="ON516_002149"
     CDS             3383..4582
                     /locus_tag="ON516_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3345620.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="ON516_002150"
     CDS             complement(4609..5877)
                     /locus_tag="ON516_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3345621.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="ON516_002151"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="ON516_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="ON516_002152"
     CDS             complement(7094..7597)
                     /locus_tag="ON516_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3345622.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="ON516_002153"
     CDS             complement(7720..8484)
                     /locus_tag="ON516_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3345623.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="ON516_002154"
     CDS             8592..8867
                     /locus_tag="ON516_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3345624.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="ON516_002155"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="ON516_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3345625.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="ON516_002156"
     CDS             complement(10434..10634)
                     /locus_tag="ON516_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345626.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11439..>11735
                     /locus_tag="ON516_002157"
     CDS             11439..>11735
                     /locus_tag="ON516_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345627.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2448 c         2076 g         3651 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacattaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 aacgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg
    11401 tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa catcttacca
    11461 tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg gttgaaatcg
    11521 ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag ttcaagcaag
    11581 gtaagactgc tcttgattat tggcaccagt ataaagaaaa taagaaaaaa tgtggtagaa
    11641 aagtcattca attacctgct catgaagtag attacattaa agagaaagtc actcttggtt
    11701 ggacgcctga cgtcattatc gggcgaaaag aaagg
//
LOCUS       DAJSNV010000083        11507 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSNV010000083 DAJSNV010000000
VERSION     DAJSNV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.086"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(51..2126)
                     /locus_tag="ON516_002158"
     CDS             complement(51..2126)
                     /locus_tag="ON516_002158"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3345628.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2128..3045)
                     /gene="glyQ"
                     /locus_tag="ON516_002159"
     CDS             complement(2128..3045)
                     /gene="glyQ"
                     /locus_tag="ON516_002159"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3345629.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3431..4249)
                     /gene="recO"
                     /locus_tag="ON516_002160"
     CDS             complement(3431..4249)
                     /gene="recO"
                     /locus_tag="ON516_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3345630.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4392..5291)
                     /gene="era"
                     /locus_tag="ON516_002161"
     CDS             complement(4392..5291)
                     /gene="era"
                     /locus_tag="ON516_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3345631.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5306..5701)
                     /locus_tag="ON516_002162"
     CDS             complement(5306..5701)
                     /locus_tag="ON516_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3345632.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5685..6161)
                     /gene="ybeY"
                     /locus_tag="ON516_002163"
     CDS             complement(5685..6161)
                     /gene="ybeY"
                     /locus_tag="ON516_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3345633.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6190..8382)
                     /locus_tag="ON516_002164"
     CDS             complement(6190..8382)
                     /locus_tag="ON516_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3345634.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8406..9377)
                     /locus_tag="ON516_002165"
     CDS             complement(8406..9377)
                     /locus_tag="ON516_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3345635.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10082..11461
                     /gene="brnQ"
                     /locus_tag="ON516_002166"
     CDS             10082..11461
                     /gene="brnQ"
                     /locus_tag="ON516_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3345636.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3096 a         2439 c         2126 g         3846 t
ORIGIN      
        1 acagagacac agcacgtttc gtgcatgctc tctgtttttc aaacaaaaga ctattttgtg
       61 atgatttgat ccaagcttgc taatgacaaa gccatggcag ctatttgact tagttgtgtt
      121 agtcggttct tacgtactgc tggttcatca gccatgacca tcgtttgatc aaaataattt
      181 tcgatcagtg gacgaagagc gacaagctgg tcgtagtttt cgtggatcgt ttggtcagca
      241 aaacttgctt tcattgcttc tactgctttg tgtaattctt tctctgcgtc gttttcgaac
      301 agctcagggt ctacttctga ttgtttttca agctgcgctt ttttcgctaa gttcacgaca
      361 cgtgtcaaag cttccattga aggtttgaag tcttcatctg caagatgttg tttgaacaat
      421 tgtgctgctg caaatacttt tgtcagatct ttttgttccg cattcaaaac agcatcgatg
      481 acatcatgac gaatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg
      541 atcacttgtt gttgcccttc tgataaacca atgccgaaac gatccgtatc tttactgata
      601 acatcgctaa tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg
      661 atccgaacga caccgtatgc ttggcgacgt aaggcataag gatcgtttga cccgcttgga
      721 atcatcccaa ctgcaaagaa agaaaagatg ctatctaatt tatctgcaat cgctaaaaca
      781 gaacctactg tagactgagg tagctctcct tcactggata taggcatgta atgctcacga
      841 atcgctgtcg ctacagcagg tttctcgcct ttcagaagag catatttttc gcccatgatc
      901 ccttgtagtt caggaaattc ccctaccatg ttggttacta gatcaaattt atagatttca
      961 gcagctcgtt gcaaatccat caattcttcc tcagatagtc ctacttgttc gccaatgata
     1021 cgggcaatga cttgtacccg ttgcattttc tcatagatac taccgatttt ttcatggaaa
     1081 gtaacagttt ttagtttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa
     1141 aagaattccg catcttctaa acgagccgtc aatacttttt gattgcctcg aaccacatct
     1201 tccaatttta cccgatcacc attacgagca gaaataaaat gaggaagcag cataccttga
     1261 tcattccgta cctcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca
     1321 ggaacagaaa gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa
     1381 ttgttgactt cttctagtaa atcttcgtct agatcaataa cccagtgatt tttttctgct
     1441 aattcgtttg cttgtgtaac gatcatttct tgtctttttt gcggagacgc aatgacgaat
     1501 tgttcattca atttttcttc atattctttt gcatgcgcaa atgtcacatc ttcgcctaag
     1561 aaacgatgac ctcgagaggc tctgcctgtt ttgacatcca agatttcgaa tgggattact
     1621 tcgtcatcta acaatgccac gatccagtga atagggcgga tatattcaaa atcgtattct
     1681 gcccaatgca tcgttacagg gaaagtcaaa cttttgatca cccctgctaa gcctgtcaat
     1741 acttcttttg cttccttacc atgagtgtat ttcgttacat agacatattc cacacctttt
     1801 aattctctaa aagtgatagc gtctgtcgtc agaccttgtc cgcggacaaa tccttccgct
     1861 gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct
     1921 tcttcactgt ccgcttgtcg ttctggaata tttgttactt tgattgctaa acggcgcggt
     1981 gtggaaaatg tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt
     2041 ttttcttcta attgtttcat gcttggtgtg acaacatggg caggcatttc ttccagcccg
     2101 atttcaagta ataagtcttt tgccatctta ttcagcctcc ttcacggttt ctggatttgc
     2161 tgtgttttta ttcaacaaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc
     2221 tacagcacga gccatgttgc ggatgcgaga aagataaccg gcacgctctg ttacggatac
     2281 tgcaccgcgg gcgtctaata agttgaatgt atggctacat ttcaagatat aatcatacgc
     2341 cggatgaaca agattttcat cgatacagcg tttagcttct ttttcaaaac gatcaaagtt
     2401 gcttagtagc aagtcttggt cgcttacttc aaaagaatat ttggaatgtt catattctgg
     2461 ttgtttgaag atctcgccat atttcacgcc atcagcccat tctaaatcat agacactttc
     2521 aacttcttgg atataagaag caagacgttc taatccataa gtgatctcag aagttactgg
     2581 tttacaaggc agtccaccaa cttgctggaa gtacgtgaat tgtgtgattt ccattccgtc
     2641 aagccatact tcccaaccca caccggcaca gcccatggat gggttctccc agttgtcttc
     2701 aacaaaacgg atgtcgtgtt ctaatggatc gattcctaaa aggcgcaagc tttctaaata
     2761 aagctcttga atattctcag gtgaaggttt catcaccact tggaattgat gatgctgata
     2821 caaacggttt gggttttcac cataacgtcc atcagctggt cggcgagatg gctctacgta
     2881 agcagcattc catggttctg gtccgattgc ccgtaaaaag gtatatgggc tcatcgtacc
     2941 tgctcctttt tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt
     3001 ttgtaatgtt aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt
     3061 caattttaaa gcgttgctgt caaataagaa tacaggaaag aaaacgacaa aaaatcccta
     3121 tgtctgatta cagacatagg gacgaataat tcgcggttcc accctacttc cgaataaaga
     3181 ttcggcagct tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct
     3241 cccactctcc cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat
     3301 tcattttgca gttttcttgt ttctataata gatcctatca ctgaacgatc tatttaatct
     3361 atggttaata atagacggaa ctccctgtgt ttgtcaaatg gttttgattt tttgttgtat
     3421 gttttattta ctattcttct ttttcaggtg tctctttttc tttttcagac atgtctttct
     3481 ctgacgtctt taatacgtgt gcccaagact tcatttgatt gatgaacttt ttgcttttca
     3541 aatggatacc gacatattca tcatacagct ggtcgatgag ttcacggatt gcttgtttgg
     3601 tttcttcttt cagatggatt ccactaattt tatcataaga aatagctgaa aacatccgaa
     3661 cgaaataaac agctcgagga tctgcatgat aacggtgatc gtccatcgac caatgtcttt
     3721 cgcatatcac accaccatat ttggaagaat agtcaaattt tccttttgtt tccccgcata
     3781 cgacgcagtg tgtccagatt ggtgcaattc cgaagcgttg cagtatttgg acttcaaaaa
     3841 tattcgtgat gatttctgca tcgtctcctt ggtccatccg ttgcagtgct tgttgcgtaa
     3901 actggaacaa atgcgggtcg tactgatgat cttcgattgc cgcatctact agattcaata
     3961 aatatgtgcc atagccgctc aaaaagatgt cttcttggat gtgtcgaaac gcatggacat
     4021 ctttgacact gcttaaaaat gacaaacctt cacttcgaaa attcccgata taaactgctt
     4081 cagtaaaagg gagcaaagcc ggagctaagg gctgattttg ccgatggatc tctttgatat
     4141 aaaacatcat tttgccagaa gattctgtaa agattttgac taatttatct ttttctttgt
     4201 aattacggct agataaaatc aagccttttg attctgcaag ctggctcata ttctccctcc
     4261 taaaaaataa atctggaaaa gcagattctg ccgatccaga tcatttatgt tgcgatcttc
     4321 cgcagctttt ctcaacacgc ggatttttat tcagtttatt tctataccta atttacaaga
     4381 agctcagaat atcagtattc gtcttttcgg tagccgaagt cttgcagata gacttttttg
     4441 tctcgccagt ctttttgaac tttgacccaa agttctagaa agactttgtc tcctaaaaga
     4501 ttttcgatat cttttcttgc tttcgttcct acttgcttca gcatttttcc gcctttgccg
     4561 ataatgatcc ctttttggct atctctttcg acaataatcg tagcctggat atggatttta
     4621 tcattctcgt ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct
     4681 cgtgttaaaa acaagacttt ctcacggatc aattcagaaa cgatgaagta ttcaggatga
     4741 tcagtcactt gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca
     4801 tccatcaaac gttcgacgtt gtttccttct gtagcagaga ttggaatcac ttgtgcaaaa
     4861 ggcatctgcg ttgaataatc ttcgatgatt cccaacaact cgtctggatg gattttgtcg
     4921 attttattga taatcagata aacaggagaa tgcacgtttt ttagacgttc aataataaaa
     4981 tcgtctcccc gaccgcgttt ttgatcagca ctaatcataa ataagaccgc gtccacttct
     5041 ttcaacgcac tgtaagctgc ttctaccata aaatctccta aacgatgctt tggtttatgg
     5101 attcctggtg tatcaataaa aatcaactga gcttctggtg tagtgtatac tccttggatt
     5161 ttatttctag ttgtctgtgc cttgtcactc atgatggcaa ttttttgacc cacgattcga
     5221 ttcaataatg tggatttccc tacatttggg cggccgacga ttgcgacaaa ccctgattta
     5281 tgttctggca tatatgttct cctttttaat taaacaacaa atgatatatt ttagggacaa
     5341 ataaaatcaa cccaacgatg acggtaaaac aagcggtcac taaaactgct ccagctgcca
     5401 tatctttgat ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccacgacat
     5461 tttcaaaaac agtattgatg atctctacta tccatacgag aaaaacagct aaaagcagcc
     5521 ataaccattc tatccgatca agttggaaaa tcacgcccat aaacaaagcg aggattccga
     5581 acgcaacatg tttcctcatg ttgcgttctt cctcaaaaac agttttgact ccttggacgg
     5641 caaattccac ggacatcagg aaatgtttgt ttttctctac gtttttatct tttaagtcca
     5701 taggcatcta agatctcttt ctgtaaacca aacatttctt tctcatcctc tggtgtcata
     5761 tggtcatacc cgttgatatg caagaatcca tgaaccgcta aaaagccaag ttcccgatca
     5821 tacgaatggc cgtaagcttc tgcttgctct gcagcacgtt cagtagagat catgatgtct
     5881 cccagtacac gaggcaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg
     5941 ccctcttctt ccaaggcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca
     6001 cgattgatta cttggattgc tgcattgtcc atgaaagtaa cagacatttc tgtttcttct
     6061 ggcaactcta aatatgatgc tgcaaattgc agcaagtcgt cgatttcttt cattttctct
     6121 tctgagactt ggtttgtttc atccatgaag gtaatatcca ttatacatcg cttctttcta
     6181 ttcttcttgc tattcttctt gttcttcttt tatcttctca acttccgatt tcattttagg
     6241 atatttgatc cgagaatgga aggtactgct taaaccgctg atcaatgatt tttctacttt
     6301 acggatttct tttaacgtca atccactgtc gtccaattgt ccatcggaaa tacgctcttc
     6361 aataagatta tggacaaaac tttcgatttt ttcgctcgtt ggatggtcca ttgcccgtac
     6421 tgccgcttca caactgtcag ctatattgac gattcctgct tcacggcttt gaggttttgg
     6481 tccaggataa cggaattgct cttccgtaac ttccgggttg cgttcctttg ccttaacgta
     6541 gaaataacgc atcagcgtgg ttccatgatg ctgtctacaa atatcgatca ccatttgcgg
     6601 catattataa tcttccaata ttttggcacc atctgtcaca tgtccaaaga taatatgttt
     6661 actgtcttct ggcaataaga aattgtgcgg attttccgct cctgaaggca agttctcaac
     6721 gaaaaagctg gcgtgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag
     6781 taaagaacgt ccaccgattt ctgctacagc attagcactt agattggcta ccatcatgct
     6841 atggtgatac gtacctggtg cttcttccag caactgtttc aataaaggat gattgggatt
     6901 gctgagttca tttaatacga tcacactatc atcattgacc atcaattcaa tataggggtg
     6961 cagacctatc gtcagcaaga atgaaaacag tgcacctgtg aatccgcaga ctagcgttaa
     7021 ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg atcaaaatga catccatcat
     7081 cacaggaaaa ataatgaccc acatggcagc agataatcct tgttctgaga tccgtttacg
     7141 tctgacaatc gttgccatga gcccagagaa aacataagac atcaagataa tcgttagaaa
     7201 gttcgtgcca atcgaatcat aaaaaatgaa aagtgcggat actgcttgga aaataccagc
     7261 catgatccct gctcttcgat tcaagaaaaa gcttaaaaca agtggtgcaa aagcagcggg
     7321 ataaaacagc gggatataag tagcttgttc cgtttggaat aattggaaaa atttcatcag
     7381 cactatgctg actgacatag ctgtcacata gaaaagaacg tattttgttc tttctcctgt
     7441 attttggaat tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa cagctaaaac
     7501 catggcaatt agcggaaaaa tcgaagttgt ttgattagtc agtcctagta attccaattt
     7561 tttcatagct gaagcatcaa tttgattacc ttcacgaaca atgatttctc cttggaagat
     7621 catcactggt tgtacagctt ctcgtgcgga ttgtttcaat tcttctgtct ttttctcatt
     7681 caatacgtca ttcactacga taccttgatc cactaagtag cgaatcattt gttgttgtgc
     7741 tggtgtgaca ttcaaatatt ggatttgctc ttctgcttct tgtttaaaag cttccaattc
     7801 actttcacga acttgtttgc tcatctgttc gtcaatgagc ttcagactct catccctcac
     7861 agtatgaatc tcagtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggtagttt
     7921 ttgataaaac acaacatttt ctgcattcac attttcaaaa tttttcttca gcgcggcgat
     7981 ccgctcttct gtagtaggct ttgggatatt ttcatcttcc tttgccttat caatcttttc
     8041 gtcataagat ttgttgatgg catcattcgt cttatcgatc aattcaaata gttgcttgat
     8101 ccgattgttc tggtcatctg caagatcttt ttggtaagtg tattctggag tgactgcttc
     8161 tgctgccagt tttctttttt gttcggtttc ttccgtgttc tcgatcgttt tgttcgcacg
     8221 gatactttct tctgctactt ggccttctct gaaatcattg gccttttgtt tgacactact
     8281 aaatgtaacg ccgcagataa tgataaaaaa caagaacagc aggaaaggca atgctgcctt
     8341 tccaagtttt tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc
     8401 aaagcttatt cttgatggag atctgccttt tcataagctt tgatgatttc ggcaactaca
     8461 gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat cgattttctt gatttgcttt
     8521 aatgttcgtt ctgcatgaac aagaccactt gttgtccctc gaggcagatc aatctgactc
     8581 gtatctccat tgacaatcat ttttgattga taaccaagac gtgtcaggaa cattttcatc
     8641 tgagcaactg tcgtgttctg tgcttcatcc aaaataacga atgcatcatc caatgtccgc
     8701 ccccgcatgt aagctaaagg agcaatttcg atcacgcctc gttccatcaa tcggttcgta
     8761 tgatctaatc caaaaatttg gtataaagca tcataaacag gacgcagata aggatctact
     8821 ttttctttta gatccccggg aagaaagcct agattttccc ctgcttccac agcaggccgc
     8881 gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag cgattgctaa tactactgca
     8941 agaaaagttt ttcctgtccc ggctggtcct acgccaaata cgatatcatg tttggctacc
     9001 gattcgacat atttcttttg gccgctgttt tttacccgta taggttttcc attacggtct
     9061 ttgacgatct cttcctcata catctcaaca aaataatcta atgtgccttt ttgccccatt
     9121 ttcagtgccg tcacgacatc tggtgtactt acatggattc ctcgtttgat caattcctgc
     9181 aatgtccgaa tgactgaggc agccaaggaa atctgggatt gttcgccaat gatctgaatg
     9241 atttctccac gtgtatggat ggttgtttct gttgtttctt caatcagttt tatgtgcttg
     9301 tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg tcaatctaat ctctaaagaa
     9361 ctagactctt ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta
     9421 ccatactcag aagaaactat atagcaaata agaaaaggtt ctaaaagagt tgcttaacca
     9481 taagtaccaa gaaacgttaa aaaatagctg cttccattct ctaaaggtct cgtttgaata
     9541 aaaaaagctg atcatgtaag ctatgcataa gatagaggac aagtattccg tctaacttag
     9601 caagtattct ctgggtattt ttcttgtttc aaaaaaagaa aacaaaatat ttgaaatatt
     9661 cttgcatttc aaaaaaaaca ggtgtattat aacgtcaaca aataaatata cagcaaagcg
     9721 tcgaggaaaa cagtaggcta ttcagtgatt tattcccagt gagtcatgta agtgagaaat
     9781 gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg
     9841 tggctgcctc cattatcagg ccagcggata actttatcga aaagcgtccg tgaatgaaga
     9901 agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg
     9961 gaaaagggct ttttttatat tttaaggtgg tggttttcat ggaatctgat attagtggta
    10021 tttctagttt ctgcaacgtt tattttgtta ctcaaaatga taattattgg aggaaaaatt
    10081 tatggaagaa acaaaaactt attcatggaa gcaattattg atggtcagtt cacttatctt
    10141 cgggatgttc ttcgggtcag gtaatctgat ctttcctgtc cacttaggtc aaatggctgg
    10201 atcaaactgg atcagcgctg gtgtaggatt tgccatctca ggcgctcttt tcccattatt
    10261 agctatctta gctgtagtag tgacaaaaag tgatggtctg tatgacttag ctaaacctgt
    10321 cggaaaatgg tatgctgcct tgttcttgat ccttgttcat ttaacgatcg gaccattctt
    10381 tggtacaccg cggacagcag caactgcttt tgaaatggct gctaaaccct ttttgcctga
    10441 aaaatatgat tcattaggta tgtttttatt ctctgctctt ttctttttac ttgcttattt
    10501 ccttactgta catccaacac gtttggtcaa atatgtcgga aagtatttaa actccttgtt
    10561 tcttgcatta ttagctatca tttttgtcgt ttctttattc caacccatgg ggaatctaaa
    10621 ccaatctgct gcagattctt atcagacgaa cgctttgaca aatggcgttt tagaaggata
    10681 caacacggtt gatgcagtag ctttacttgc tttaagtgtc acttttgtcc atgcagttcg
    10741 tgatcttggt tttaaagata aaaaagtcac agaattaaca gcaaaagctg gtagcctgag
    10801 cgttgcgtta gaagtcatga tttattttgg tctggtcctt ctcggtgcga tgagtttgaa
    10861 taagatgtct ttatcagaaa atggcggaac tgcactttct cagatcacta atttttattt
    10921 aggtaacttc ggtgctagct tcttaggggt aatggtcaca ttaggtgtct tcactaccgc
    10981 tatgggactc gttgtttctt ttgcacaaga tttccataaa ctgttcccta aagtcagtta
    11041 tatgacatgg ctgcggttaa cgacttttgt ctcttttgtc gttgcgaatg ccggattgga
    11101 taatatcatc caatggtctt taccagtatt gatgcttttg tatccattgt cacttgcgtt
    11161 aatcttgctt tccttaacag caaaattctt ccaaaagagt ccatttgtct atcaagtaac
    11221 gatgttcttt tctgccgttc cagccgtatt ggatatgttg gcaaattcgc ctgcattagt
    11281 gagtcagcaa actgtcgtag caagcttact aggattttat catcattatg ttccttttgc
    11341 tgcattagga cttggctggg tcgtcccaac gttgcttggt tacgctggca gcttgctctt
    11401 ttattatgta tatcgcgtat caggatataa acaagaagca agtgagttac cagaagaata
    11461 accaaatcaa acacaattta acataagaga tattaaacag gaaaaaa
//
LOCUS       DAJSNV010000084        11153 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSNV010000084 DAJSNV010000000
VERSION     DAJSNV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.087"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            60..1511
                     /locus_tag="ON516_002167"
     CDS             60..1511
                     /locus_tag="ON516_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3345637.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1860..2492
                     /locus_tag="ON516_002168"
     CDS             1860..2492
                     /locus_tag="ON516_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3345638.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3080..4519
                     /locus_tag="ON516_002169"
     CDS             3080..4519
                     /locus_tag="ON516_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3345639.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4814..5032
                     /locus_tag="ON516_002170"
                     /pseudo
     CDS             4814..5032
                     /locus_tag="ON516_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5281..5496
                     /locus_tag="ON516_002171"
     CDS             5281..5496
                     /locus_tag="ON516_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345640.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5713..5969)
                     /locus_tag="ON516_002172"
                     /pseudo
     CDS             complement(5713..5969)
                     /locus_tag="ON516_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6156..7067)
                     /locus_tag="ON516_002173"
     CDS             complement(6156..7067)
                     /locus_tag="ON516_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3345641.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7086..8090)
                     /locus_tag="ON516_002174"
     CDS             complement(7086..8090)
                     /locus_tag="ON516_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3345642.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8087..10210)
                     /locus_tag="ON516_002175"
     CDS             complement(8087..10210)
                     /locus_tag="ON516_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3345643.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10215..11135)
                     /locus_tag="ON516_002176"
     CDS             complement(10215..11135)
                     /locus_tag="ON516_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3345644.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3446 a         2104 c         2080 g         3523 t
ORIGIN      
        1 tatttatcat aatttttaat agaaaacact attaaagaga aaaaagagag gtaaatcaaa
       61 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
      121 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      181 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      241 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      301 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      361 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      421 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      481 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      541 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      601 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      661 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      721 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      781 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      841 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      901 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      961 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
     1021 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1081 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1141 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1201 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1261 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1321 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1381 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1441 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1501 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1561 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1621 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1681 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1741 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1801 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1861 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1921 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1981 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     2041 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2101 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2161 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2221 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2281 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2341 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2401 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2461 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2521 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2581 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2641 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2701 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2761 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2821 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2881 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2941 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     3001 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3061 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3121 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3181 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3241 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3301 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3361 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3421 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3481 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3541 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3601 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3661 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3721 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3781 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3841 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3901 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3961 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     4021 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4081 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4141 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4201 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4261 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4321 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4381 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4441 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4501 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4561 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4621 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4681 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4741 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4801 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4861 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4921 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4981 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     5041 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5101 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5161 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5221 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5281 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5341 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5401 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5461 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5521 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5581 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5641 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5701 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5761 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5821 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5881 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5941 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     6001 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6061 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6121 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6181 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6241 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6301 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6361 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6421 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6481 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6541 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6601 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6661 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6721 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6781 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6841 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6901 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6961 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     7021 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7081 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7141 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7201 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7261 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7321 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7381 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7441 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7501 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7561 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7621 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7681 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7741 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7801 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7861 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7921 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7981 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     8041 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8101 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8161 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8221 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8281 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8341 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8401 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8461 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8521 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8581 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8641 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8701 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8761 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8821 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8881 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8941 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     9001 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9061 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9121 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9181 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9241 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9301 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9361 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9421 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9481 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9541 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9601 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9661 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9721 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9781 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9841 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9901 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9961 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
    10021 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10081 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10141 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10201 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10261 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10321 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10381 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10441 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10501 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10561 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10621 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10681 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10741 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10801 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10861 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10921 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10981 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    11041 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11101 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc acc
//
LOCUS       DAJSNV010000085        11128 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSNV010000085 DAJSNV010000000
VERSION     DAJSNV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.088"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ON516_002177"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ON516_002177"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3345645.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ON516_002178"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ON516_002178"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3345646.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="ON516_002179"
     CDS             2139..3020
                     /locus_tag="ON516_002179"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3345647.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="ON516_002180"
     CDS             3017..3733
                     /locus_tag="ON516_002180"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3345648.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ON516_002181"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ON516_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3345649.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ON516_002182"
     CDS             4450..5451
                     /locus_tag="ON516_002182"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3345650.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ON516_002183"
     CDS             5448..6809
                     /locus_tag="ON516_002183"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3345651.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ON516_002184"
     CDS             6769..7920
                     /locus_tag="ON516_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3345652.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ON516_002185"
     CDS             7895..9064
                     /locus_tag="ON516_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3345653.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ON516_002186"
     CDS             9239..10618
                     /locus_tag="ON516_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3345654.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="ON516_002187"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="ON516_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3701 a         1946 c         2495 g         2986 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAJSNV010000086        11007 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSNV010000086 DAJSNV010000000
VERSION     DAJSNV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.089"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(157..309)
                     /locus_tag="ON516_002188"
     CDS             complement(157..309)
                     /locus_tag="ON516_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345655.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(618..1031)
                     /locus_tag="ON516_002189"
     CDS             complement(618..1031)
                     /locus_tag="ON516_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3345656.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(1108..1404)
                     /locus_tag="ON516_002190"
     CDS             complement(1108..1404)
                     /locus_tag="ON516_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345657.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(1401..1958)
                     /locus_tag="ON516_002191"
     CDS             complement(1401..1958)
                     /locus_tag="ON516_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3345658.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(1955..2374)
                     /locus_tag="ON516_002192"
     CDS             complement(1955..2374)
                     /locus_tag="ON516_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR3345659.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(2371..2589)
                     /locus_tag="ON516_002193"
     CDS             complement(2371..2589)
                     /locus_tag="ON516_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345660.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(2576..2932)
                     /locus_tag="ON516_002194"
     CDS             complement(2576..2932)
                     /locus_tag="ON516_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3345661.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(2932..3237)
                     /locus_tag="ON516_002195"
     CDS             complement(2932..3237)
                     /locus_tag="ON516_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345662.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(3234..3395)
                     /locus_tag="ON516_002196"
     CDS             complement(3234..3395)
                     /locus_tag="ON516_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3345663.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(3392..3694)
                     /locus_tag="ON516_002197"
     CDS             complement(3392..3694)
                     /locus_tag="ON516_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345664.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(3687..3851)
                     /locus_tag="ON516_002198"
     CDS             complement(3687..3851)
                     /locus_tag="ON516_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345665.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(3856..4125)
                     /locus_tag="ON516_002199"
     CDS             complement(3856..4125)
                     /locus_tag="ON516_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345666.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(4137..4886)
                     /locus_tag="ON516_002200"
     CDS             complement(4137..4886)
                     /locus_tag="ON516_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345667.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(4889..5575)
                     /locus_tag="ON516_002201"
     CDS             complement(4889..5575)
                     /locus_tag="ON516_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345668.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(5581..6252)
                     /locus_tag="ON516_002202"
     CDS             complement(5581..6252)
                     /locus_tag="ON516_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3345669.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6245..6586)
                     /locus_tag="ON516_002203"
     CDS             complement(6245..6586)
                     /locus_tag="ON516_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345670.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(6764..7462)
                     /locus_tag="ON516_002204"
     CDS             complement(6764..7462)
                     /locus_tag="ON516_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3345671.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7549..8019
                     /locus_tag="ON516_002205"
     CDS             7549..8019
                     /locus_tag="ON516_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345672.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8062..8241)
                     /locus_tag="ON516_002206"
     CDS             complement(8062..8241)
                     /locus_tag="ON516_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345673.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8228..8566)
                     /locus_tag="ON516_002207"
     CDS             complement(8228..8566)
                     /locus_tag="ON516_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3345674.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(8582..8785)
                     /locus_tag="ON516_002208"
     CDS             complement(8582..8785)
                     /locus_tag="ON516_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345675.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(8800..9576)
                     /locus_tag="ON516_002209"
     CDS             complement(8800..9576)
                     /locus_tag="ON516_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3345676.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            9875..10195
                     /locus_tag="ON516_002210"
     CDS             9875..10195
                     /locus_tag="ON516_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345677.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10213..10644
                     /locus_tag="ON516_002211"
     CDS             10213..10644
                     /locus_tag="ON516_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3345678.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            10774..>11007
                     /locus_tag="ON516_002212"
     CDS             10774..>11007
                     /locus_tag="ON516_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345679.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGIL"
BASE COUNT         3048 a         2241 c         1700 g         4018 t
ORIGIN      
        1 gttagttaaa actaatttga atccaaatca aatgtgtgaa aaattaaata cagtacttga
       61 taataacgat tccttattca tctgtgaact gcaaaaaaat tatcaaggta gagctacaga
      121 ggaaaattgg aaattcatta acgaacatat tttctcttag taaggattag atattttttc
      181 tctgtttaaa caattgcaaa taccgtcaat atctgaatta gaaacactta ccttttgctc
      241 cttgctacta ccaatagctt ggagcaattc ttgtatttct tctggagtgc cttctactga
      301 taatttcatt tttcttccct ccaatacata aattaataga cagcagcagt tgaaagacga
      361 caagaacatg tttagttttg tgagtgctgc tgtctaatca aaataaacag caaccgatga
      421 agaatttagg aggagttgaa ttcacatcct tttcttcata attagttgct gtctatctaa
      481 gaagaagttt aaaacgatga gggagattgc ctcccttcgt ttattttgtc gatcctgttt
      541 cctaatcttt cgacactacc ataataacac tggtaaatgt ctaaaaaccg ccatcattcc
      601 gccaaaaaac cgccaaatta tttataagca attatttttc cgtgtttata tgcttctgca
      661 aactctatta gagcttccga cttcatccgt tgtatacttc tttctgaata acccacttca
      721 cggctaattc tgtagtttga gaagctatct ggcacacaga agctgtagta gagtatctga
      781 cgactaatca gactaagagc catcaaagcc gctagaatcg catctctctc cgtttctata
      841 tccatcatct gaataatcgc gtcttctgcc ttattgccat gcttcggtgc cttcggcata
      901 tccgttataa tcggcgactt aatatctatc aaagagcgac ctgccatccg ctccaaacgc
      961 cgaaagttct tcagcacatc tctcgcatta catcttgtct gtttgaaatc tacctctcgt
     1021 aacaattgca tcaagtcaaa ccgctccttt atgtgatata ataaatgtgt tggatttatt
     1081 gaatcagtcg gagcgatccg gcttttttta tttgctcttg attacttcca tgtcaactaa
     1141 tctcgctaca gctaaataat gcttgtgttt tgcagtcgaa cgatcacact tcattgtatt
     1201 ttcaatacga atgattgctg agtttgacag tatcttttta acgtatcctc tgaacggata
     1261 aacgaactct tctgcttcac agcggaccat gtcaccgact ttgaattttg atttcttgcg
     1321 tgttttaggg ttctttgtcg gcatatctag cattaaaccg ccgataccgt gactgctagc
     1381 gtaaaatccg tcttttagtt tcatctttct accacctctt ccactgttcc attcaccaac
     1441 aatgctgcag catccgctgt tgctttatca gtgaaaagca atcgatgaga tggattgcat
     1501 tcaaaactat aatttactcc atcttttccc cataaagcac atttagtaag atagtttcca
     1561 tcggcattct ttattgccca taacggctct ttctcaacct cgtagccgtt gtataggctc
     1621 aatagtgttt catatgattg tcttgttgcc caagcgagta attcttctcc ttgctcttgc
     1681 gtcactatgc catctcgtac aaaccaatca cagaaataat atgaatcacc atctttagat
     1741 ttgattaaat acgctatttt ttcagctttt gtaaaagggt ccgtagattt ctcaagccaa
     1801 ccggccacga gttgtggaat aactggtttc tgcggttcgt ctattttttt cattaacgta
     1861 atacctgaat ttatactctc gttatagcaa gcagctattg catcatttcc tatcgcttta
     1921 atactttcta actttttgat tgcttcctgt ttattcatcg ctgttcctcc aataactcgc
     1981 tattctcgta tatatttccg gtgacttcgt acgtgtattc ttcaaacagc tctgtgttaa
     2041 aaatttgata ctctaagtct tcatcaacag tggcacaaac taatccggaa taatctattg
     2101 aatttttgac aatacagact ttattatcta agtaatcgaa gccatttcgc acgctgacta
     2161 atactatatc cccttcaaat atatccacac cattcttatc tttcattcct gtggattgca
     2221 tgaggtcgat gtttcgcacg tgatatgatt tcaattcggt aggatttatc caatattcaa
     2281 taaagtttgt tttgccgttt ttagtaaaat gcaagacggc tacatctctc attacgttct
     2341 ttcgtttatc ccacgctcta aacttcggta tcattcgctg tcctcctcgt atttttcaat
     2401 caattccatt acttttttca ctatttcaaa ctcaaccgct tttgattctt cgaaatcatg
     2461 aacaatttct ggaaacagta catcatcaac tacccacaaa ataatctggc gctttccacc
     2521 aaaatttatg attagatggt ccgattccac agatagtgtt gctcctgatt cgatatcata
     2581 taaatccatg ctgaattgaa cgagtttttt tatcatttgc tgtcctcctc aaatactctt
     2641 ctaatatttc tttatacttc tctacaaatt tgaaacgatc ttgatgaagt ttcttgctcc
     2701 aatttgtttg ccgatccagc tcacgcatct gatcgaaccc tttttgaatt tcgttgtaat
     2761 aaaattcaat gtttgctgct gctttccaat gcctgctact tcgcactcct gctcctgttt
     2821 cagccatttc taacttaact aattcagctc gttcttttga ttttttatct ttctgaatct
     2881 tcatcatgat tttcttgagg atgatgtcac tgtattgtgt aatgagatcc attatctctc
     2941 ctccacatac ctaaactgtc gtccttttga atcaatccat aagctcctag ctctatccca
     3001 gataatgttt ttgcttaatc cagtaatttc agataactgt tcagcagtac ctgttactaa
     3061 aattcggtca tcatgccaga ttgcaatttt tctcggcgtt ctccgtttgg gcttttcagt
     3121 ccacattgat ttaccgagct tttggacttc tgcaactatt tctttgtctt cttgccaaga
     3181 ttctgacttg gttaattcag caattctttt cattgctgct ttcttatcca cgctcattcc
     3241 tccaatcgat ggatttccct tcttaaattc tctatgtgca aatcgattgc ctttctcgcc
     3301 gtttcattga ccatcactgc ctttgttcgt tccagatcgt caatctcacg ctgaaggctt
     3361 cgaatacgca tttgaatcac ttcttctgtt gtcatgatga ttcctccacg tatctaaacg
     3421 ttctcttctt agcgtctgtg tagccacacc ttgctctttt cctcacgatt ttctcgtgca
     3481 agcctgtgag acttgctaac tgttcagctg tccctatcac tagaagtttg tcaccgtgcc
     3541 aaattgcgat ttttcgtggt cttggcttgt tgctcttgtc tgcccacatc gctcttccaa
     3601 gcctcatcac ttctgaagca gcttctttgt cattttgcca atcttctgaa taagtcaatt
     3661 cgataattcg ctgcattgcc gctttcttat ccatcccgac attctccttt cagtaatttg
     3721 agtacttgat caagtgcgct ctcacgtccg ccatggaacg tgttgagcca cttgtcttcg
     3781 tacgatacac tttgtcttaa agcttcttga tgcattagtt caatctgtgc tgtaaatgtc
     3841 tttaggtcca tctgattaca cctgctcaag ttcattaaga tgtttttgca agcctttaac
     3901 acaatcaaca aatagtaatt tggtatatgc taaatttctt aattgtgttg tatcgatata
     3961 aagtgcgaaa tagtatctga gcttactcca actcgaacga tcattcttaa ttccttcaat
     4021 tccagcttct tcgagttgat catatacgtc tctcaggacg tctaattcct cgcccttttt
     4081 ataattcgct atttcattaa ttagttctag ataatcgatc ttcatttttc cacctcttag
     4141 aatggtgctt ttgattgtct attagctcgt tctagcgctt ttttcttgag ataggcttct
     4201 tggtcgattg cccattcagg aagcttctct cgtcttcctg tgcgcttgta tccactgctt
     4261 gcgttcttag gttcactttt ttctttcctt gcccaacttc gaatagttgc caaatagttt
     4321 ttataagtct taccagatga ttcacaatac tctgacagtc gttcgattcg ctcttggtaa
     4381 tcattaggga attctgtttt gagtttctcc atctgctcat ctgacaaaag aacattttta
     4441 tactctccgt atttatgacg gacgggctta gccttcgatt ttttcgaagg cggtaactct
     4501 cttatatatt cttttgtatt attaaatgta ttattaatag atgtattatt atctttgact
     4561 ttttcgtcaa taggggtatt gcgtttttcg tcaatagggt attgattaat tcgtaggtac
     4621 ctattgatta tttgattggt accctctttg taaatgattt cccgattcaa gtatccaaac
     4681 ttaatcaaat cacttaccca tcgcgatatg gtctctttat tcacaccata taaatctgca
     4741 aagtactcat tgcctgccca acaaaagcct ctttcattac acaaggccgt tatctctccg
     4801 tataacaact tagtatttgg tttaagtctt ttgtcgtacc ttacgttggc tggtataatc
     4861 gcataataac ttcgatgttc tgtcattttt accctccaat atttaacttt ttgattgttt
     4921 tctggtttaa tttgatccct ttgatttgat atttattttt gaaattaatc acacctattt
     4981 tgtgcttctc tgtgtgatgg attctgcaga gtgctgcaaa tgtgtactct gcatgatcaa
     5041 cttctttgcg ctttcgtctt cctagcgctt tgtcaaagtg atcgatgtca gctcctgttt
     5101 tgccacagat gcagcagact ctttttgtga tgcatttgta gaagtaatat tcttgattcg
     5161 caggtaaaat ctcatagcct tctttgaaag gaatatgatg ttcaaagatg aaatctaaga
     5221 tgatatttgc taagacattg gcatcactca cagttgtatt cgattcgtct ttcaggctta
     5281 ttttgcgccc tgtgacacct tcaaaacgaa agtagaagaa ttccttccag aagtccgttg
     5341 gcatgcctgt atcgataaaa atatcgccta tcagcgcata gatgaagttt cgttgctgta
     5401 cggtgaaacg tctaggatca ataaaacgaa tttcaatgac tcgatcacca tcgtagccgt
     5461 catacatcgt ctttagtcga tcaatgttca cttcctcatt gatggttgcg cttatgtctt
     5521 ttcctttgaa ctttttcaga accgctgaat atgaatcgat taatggttta aacactcata
     5581 tcacttctta tctaattctt ttctcttagc tgctattgct cgctccatca aggcacattg
     5641 ctcatagctt aactgttcaa tagtttcaac gttatcagct aagagcccta atttatctgt
     5701 ctgctcatta acatattcga ttaaggtttt ggtcatatct ttacccatct gctcattgaa
     5761 agcttctaga atcgtctcta gcatgtttaa tttctttgta tcgattctag gtggtgttgg
     5821 aatatcttcc ccttgaaata catataatcc cagtccgtgt agagccaatg ctttcacaaa
     5881 gcatcgcttc aatgagttat tgatttgcat tgcatttggt ttaacaactg gttggtttcg
     5941 ataatctaaa acaggaaata attcggtttc cgtgtgtcct ttaaccgtta ctgagacaga
     6001 tacataagtc ccagtttcat ccataagaaa aggtttatat tcctcaacaa gaaagtcttg
     6061 atgagttcca gaaacaaccc tgtagtgttt gtactcatta atagttaccg ttgcctgtgg
     6121 atcattcttt ttcataatct cccacgcgtg agcccaagat aaataatcaa aatttccttt
     6181 tttcttgaga attttattta acttacgact aaaaagtttt tcaaagttcg ttgtcccttt
     6241 gatttcactc atcaaattct gcctccattt cagcaatgta tttcttacct ggtccgtaat
     6301 aagagatatc aatcaagtta tctctgtcgt actcttctag cgcatcaatc aagccatctt
     6361 cgatgacgta aatatattca ggtttattcg aatgcttcga tagatggata agataaacat
     6421 gatcccaaat agttacatgg ttgcctaaat catcttgatc ccaagctagt tcttcattcg
     6481 tcaaaagatt tcgtctgatt tttcgaccac ttgtttcctc aattttcggc ttgccccaat
     6541 caggatcaat caaatattga tctagagtgg aaagttcttt ttccatatgc taaaatctcc
     6601 ttatgatgtg ttttctttgt gactctttgc ttgccggcgg agtcactttt ttatttgttg
     6661 ccatgctttt tgcttgtcaa tatgttgttg gcttaggatg attggtttat agtatttcca
     6721 ccagcaatta gcaattgccg tccctattct tagcgcttca gctctattca ttgtcatcac
     6781 caaaaagtct ctgttgtctg ttcagttgat caatttccat acggatcgca gtttctggca
     6841 accacatttc aataaatgaa acagcatcat cgaatctctt acgaggtaac tcgccatatc
     6901 ttgggattga aaaggtacgt ttaaattcag accaaaattt tgagaatact tttttgctga
     6961 tttcttcata agctcggctt tcttttcccc ctagaactcc cataactttc atatttcctt
     7021 tttgcttaat ttcaaactct tgttgtccgc taattcgcat agtatcttta agcatggaaa
     7081 catctttttt aacatctttc atttcttcta attgatagat catcatatct tcaattgttt
     7141 gaggaacagt attcttgcga ataacatctt ccatttcgtt gaatgcttca atgtattttt
     7201 gtttgaagta gatagctttc tttcctgtaa accccatagc cagcaagaaa aatccatctc
     7261 tactaatgaa gaaaactcgt cgatttctgc catatgaatc tggttcatta ccttctacaa
     7321 acatctgtcc aaaattggac acatcttttt ttagtgcatc aatatctctt aaaacatgtt
     7381 gatgtttttt ctcgaagctt tctgccactt gtaagctcgt agtcacagct tctttatttt
     7441 tcaaaattac taattcttgc attatttctt ctctcctttt tggtataatt tagttaaaaa
     7501 ctggtggtgt ttattttgaa tttttttcac ttaacaattt ttcaatggat gtcaataata
     7561 agttttatat tgagtacgtt tttaacaggg ataaaaataa aaggaatgcg ccctcaacta
     7621 gaagttgaac ttaatgcttc ttacttcgct gcagatatga tttatacaaa agtaatcatt
     7681 tctaactatt caacagaacc agctatgtta gttaatttag aactttcatc cgctaactta
     7741 aaccgcagat ggtcagctac tccatttaag aaattaattg ctaaaggagg atcagtcgat
     7801 gataatagaa tatactctga agctgtaccg ctaaatattc ctcctaaaag tgctatatct
     7861 tgttaccttg catttgaagt agggaaaatt aattttaata aactccttaa tcaccaaaca
     7921 aaaatgatat ttactctaaa tagaacccaa atccacaaaa ttgttgatat caaaaataca
     7981 aacttcccaa tagagaaatt agtgaaagaa ttaaactgag taatagtaat atacattcta
     8041 gtttctcttt tctattcatt tttagtcagc cccctcggtt ggctttttcg ctctgtactc
     8101 agcttcagct tcatcaagcc ccataaaaat ccaaaccatg taaacaatcg ttcctatcaa
     8161 cgcttgcctg cttccccaaa gtcctaaagc gtagatgatt agcggtgcgc tgaacactag
     8221 cgctctatta aattttccca tccgcttgcc tccttaagat ttccgaaaaa tttgtttcta
     8281 aaaattcaag tgttttactt cttaaaaata gatatgtgtc tcttccttca actggataat
     8341 agacaaatcc atttttgttt ttctcgatat cgataatatt tctatatctt gggttcttta
     8401 aaactctaga agtaaaccaa tcatattttc tgttaatccg ttctagcact tctggcaacg
     8461 tcatccatct accagtatca tcagcttttt ttaactcctc ataatccact tgggagataa
     8521 ttacatagcc ttctggaatt gggatttttg cttctagata ttgcattagc tgttcctccc
     8581 tttacaattc gtacatagtg ataatcgaat ctataattct gtttgcttct gcagaagtct
     8641 ttttaccgtt taaaattaaa gataagtagc ttttgctaat tccaaatctt tcagcaagca
     8701 tggtgtaagt taagaacttt gaactttcga catattcttt gattttttct ctatctcgtt
     8761 gagtgatttc tgcaatgtca gtcatactaa aactcctttc taaccaattt cctctaaatc
     8821 catttgaggg taatatcctt ctttttttag taattcgtag ataaatagac gccctttctg
     8881 tgtccatttg gtattcatta caattttagt gccaccatcg gctttcggga tctcagttgt
     8941 atgagatttt gtgtatcctt ggttcatgtg ttttttgcac aataaccatt ggttaccgac
     9001 ttttttctga atacctagtt tatgaagtaa tttattcatc tgttgtggag acatcccata
     9061 atctgctgca atctgactaa ttgttactga atctgtagaa gataatatgc tatctaaata
     9121 ggagatttta ggttcgtact ctgcaatctt ttgttctgcg attaatcttc cagttctttc
     9181 ttcttttagt tgagttgcta attgaatgat tgtatctgga ttaagcaaag cttcttctac
     9241 tttttctgga gttagataac ctccatgttt tctaattgct ggcaacactt cacttgttac
     9301 ccaacgttta aattttttgg cagagggaag ttttgatttt aagattaaac tgtacaaacc
     9361 agattcgttg atgattgtca tctctcttga ttgacctgag gtcgtgattc gcgaccccat
     9421 cttatcttcc aaatctacat gccgcgacaa agcatcttta gtgtttgaat aacccaaaac
     9481 actcgcaacg tctttgccta caaaatatgg ttcatcattt actaaaattg tccgaacttc
     9541 gttttgttcg aaattaaaaa tttgcggtgt gttcatattt ctcattcctt tcttttatat
     9601 tcgtaaacaa atttaacaac ttttttctaa attcaattga cagtatctga gtaatactct
     9661 atactaagag cataattaaa taagacataa aaacattgat tttaaagctt tcttggcggt
     9721 ctgcatttac atatcaatag tgttttttgt tgtcttttta gttgttaaat ctgtttacaa
     9781 gatatagtat agctcattac tcagaattag tcaaattatt tctgcgtatt tttctaaact
     9841 ttttttgtaa acatttagaa aggttgtttt atcgatgaat acctatgaaa taataaaaga
     9901 cttggcgaag aggaaaaaga tatctatacg gcagttagaa attaattttg gatattctaa
     9961 cggttatcta gcaaagtgga aaaataatac tcctaattca gatgaattaa gtcggattgc
    10021 tgactatttc gatgtctctg tagactacct tctaggtaga gaagaaagag aaacccctaa
    10081 acatgtggat ttatcagaag acgatactgt tttttctttt gatggaaaag aaatatctaa
    10141 ggagacaatg cgtaaagcga ttgcaattgc taaagcttta gaggaaaatg aatagttgga
    10201 gtgatggttt gtatgtattt gaaattgaaa gaaatgttga gcgaatacaa tttgaaagta
    10261 gtttatatgg agatgaaaga gcctggtttc tactatccaa aacccagaat catttttttg
    10321 aatgaaaatc tatacggtga aactgcagaa gcctttcatt tatctcatga acttgcacat
    10381 ttcagcgcct ctcattttga attttcagtt ttgtacgata cttctacaac ttttcactca
    10441 aaatttgaaa cagaagcaga taaaatagct attctaattt tattaaatat ctatattgag
    10501 aacgaactga ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt
    10561 cagaataagc tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat
    10621 tcttacgcaa ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca
    10681 aaacattaaa aaagcccgtg ctgcaacacg ggctctttcc tcatatatga gcttctacaa
    10741 aaaaaatcat atcacagaaa tgaggaagaa aaaatggaaa aagagatttt aataaaatct
    10801 ccaggaaaaa ccttgattaa agtaactgat gattctattt caattattag aaaaggattt
    10861 ataaatttag ttaatcaagg tattaaagga gaaaaaacta tcccctttaa aaatatatca
    10921 gccgtacaat taaaaaaacc aggtatgagt aatggttata tacaatttac cttgctaggc
    10981 ggaaacgaaa gtcgcggagg tatatta
//
LOCUS       DAJSNV010000087        10893 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSNV010000087 DAJSNV010000000
VERSION     DAJSNV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.090"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="ON516_002213"
     CDS             complement(<1..132)
                     /locus_tag="ON516_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3345680.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="ON516_002214"
     CDS             542..841
                     /locus_tag="ON516_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345681.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="ON516_002215"
     CDS             914..2275
                     /locus_tag="ON516_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3345682.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="ON516_002216"
     CDS             2297..2557
                     /locus_tag="ON516_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345683.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="ON516_002217"
     CDS             2571..3323
                     /locus_tag="ON516_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3345684.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="ON516_002218"
     CDS             complement(3417..4022)
                     /locus_tag="ON516_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3345685.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="ON516_002219"
     CDS             complement(4075..5049)
                     /locus_tag="ON516_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3345686.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="ON516_002220"
     CDS             complement(5427..6146)
                     /locus_tag="ON516_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3345687.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ON516_002221"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="ON516_002221"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3345688.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="ON516_002222"
     CDS             7697..9094
                     /locus_tag="ON516_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3345689.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="ON516_002223"
     CDS             9175..9615
                     /locus_tag="ON516_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3345690.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="ON516_002224"
     CDS             9763..10089
                     /locus_tag="ON516_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3345691.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="ON516_002225"
     CDS             10090..10839
                     /locus_tag="ON516_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345692.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3480 a         1921 c         2246 g         3246 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctg
//
LOCUS       DAJSNV010000088        10753 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSNV010000088 DAJSNV010000000
VERSION     DAJSNV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.091"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1591)
                     /locus_tag="ON516_002226"
     CDS             complement(<1..1591)
                     /locus_tag="ON516_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3345693.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
     gene            complement(1733..1957)
                     /locus_tag="ON516_002227"
     CDS             complement(1733..1957)
                     /locus_tag="ON516_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3345694.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2208..3794
                     /locus_tag="ON516_002228"
     CDS             2208..3794
                     /locus_tag="ON516_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3345695.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(3860..5635)
                     /locus_tag="ON516_002229"
     CDS             complement(3860..5635)
                     /locus_tag="ON516_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3345696.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5848..6750)
                     /locus_tag="ON516_002230"
     CDS             complement(5848..6750)
                     /locus_tag="ON516_002230"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3345697.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            7029..9374
                     /locus_tag="ON516_002231"
     CDS             7029..9374
                     /locus_tag="ON516_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3345698.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(9711..10712)
                     /gene="ccpA"
                     /locus_tag="ON516_002232"
     CDS             complement(9711..10712)
                     /gene="ccpA"
                     /locus_tag="ON516_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3345699.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         3265 a         2143 c         1984 g         3361 t
ORIGIN      
        1 ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta tgcaagatag
       61 cttcttctgt tttggcatct actgcagaaa gtgcatcatc taacattaat aattcaggat
      121 ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc
      181 cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca ttgttgataa
      241 atgctaaaga agccgcatgc tttacttctt cttctgaaag ttttggatta gaaaaacgga
      301 tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag ccgattgatc
      361 ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg attttccctt
      421 gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc gcaccagttt
      481 tcccaacaat acctaatgtt tctccttctt ggattgcaaa cttgatttgt tctaatgctg
      541 gtgattcttc tttcagataa ttaaaagagg aaatagctac attcagctcg ccttttgccg
      601 gggtctgcac tgcacccagt tgttccacga tatgcgtttt ttcatgcaaa agttcattga
      661 cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca atcgcaaaca
      721 tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg atcgagatcg
      781 tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa
      841 tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa
      901 ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt ccaaatgttt
      961 taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta gaaaaagctt
     1021 cttgcgaatc acgaaaagca tcatggagtt tcgatcccag cactcgtgag gtgatggcca
     1081 gcaaaggcag aggaatcatc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg
     1141 caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata ccagctcctg
     1201 ctacattctg gatcgcattc aaatcattcg ttgcatgagc catcaaatcc ccagtccgat
     1261 atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc tgccgaagtt
     1321 ctttctccaa ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat
     1381 attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc tttattgata
     1441 aatttttatc ggcaatctca tcaatgatga tcccaatcac ttttggagga actaactgta
     1501 ccaaggccac tgcgaatagc gagaaaactc caattaaata atgtttcttt tcctgacgaa
     1561 aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc
     1621 ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa caaattacga
     1681 aataaaaaac gggctgaaaa aatcagcccg taaaaagtcg ttgggtcaga acctattttt
     1741 tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc
     1801 ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat
     1861 agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa
     1921 taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct ttctatttcc
     1981 gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt
     2041 agtttcatgc aaaaaatagc ggttttttta gaggagaaca aaatttcttt cgtcatctag
     2101 caagacacgc cacgaaaata aattaggcat accgaatctt tttctttaat tttttctttt
     2161 tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat
     2221 ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta tctttagctg
     2281 aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca cttcttgctt
     2341 actctggacc tggtgcgtta gtagcagttg ggtatatgga tccgggtaac tggtctactt
     2401 cgatcaccgg aggacaaaac ttccaatatt tattgatgtc agttattttg atgtctagtc
     2461 ttattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc caaatggatt
     2521 tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta tggatcttga
     2581 ctgaattagc cattatggca acagatattg cagaagtaat tggtgcagca atcgccctct
     2641 acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta gatgttttcg
     2701 ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt gtctgcttaa
     2761 ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca gattggcaca
     2821 gtttatttgc tggctttatc cctaacagcc atacattctc agactcacct gtcattggtg
     2881 gcgaaactcc gctaactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
     2941 tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac gaagaagatg
     3001 tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca atggcttttc
     3061 tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt tctgtccaag
     3121 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
     3181 ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta gcacttcttg
     3241 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat
     3301 tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg ctttctgtga
     3361 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
     3421 ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg ccattttcga
     3481 tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga tttaaaaatt
     3541 cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac ttgaacttac
     3601 gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc gctcaactgg
     3661 cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
     3721 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag gaaacggtag
     3781 aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta ggttaaaaat
     3841 ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt aatttactga
     3901 aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca ccagtgtaga
     3961 cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag acaccgaaaa
     4021 gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa ctataatagg
     4081 cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct gctattgaag
     4141 caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc gcgacttttg
     4201 ctgattttgt taattggaag ttaggtgttc tcggttcatt actatcgatc caattctcca
     4261 ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt ccgaaactat
     4321 aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct gattgaagga
     4381 aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc atcagataga
     4441 actctttttt ctttttgatg gttcccttgt gataggcttc ttctaattct tggttgactt
     4501 ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat tcgcttacta
     4561 catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt ccaccttgag
     4621 tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg aatgtaggag
     4681 atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg ttttctttat
     4741 tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca aattctccac
     4801 tcgaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat tcgatccact
     4861 gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc agccattccc
     4921 gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa gaaaagtctt
     4981 gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa ggaagaagcc
     5041 agatagaatc ttcgcgaatg aaccaaaaaa aaggaaaagc gacagaaagc cctaaaaacc
     5101 atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat aaacaagcga
     5161 taatgatctc aacagccgga atcacgaggg cattacgata cgttcgagtg gaatattcat
     5221 gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga agatacttgt
     5281 tcttcacgat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata ctgaacatgc
     5341 cgaataaaac tgcataagaa aaatgcagtt tatgtgcaag tactaaaaat aagctgtagg
     5401 agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg tccaaaatat
     5461 tttgagcata gcgaatcagc aactgatcat cataggttgc cgacatgtct gttccataga
     5521 ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg ccaagaataa
     5581 agtatgtatg tttcttagtc agttggttga tcaagggttt taaagtgttt agcatctttt
     5641 cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgtttttt tacttttcta
     5701 ccagtatacc ctgtttgtct aaatttaaaa agagggggga gaagatgcat gatgaaaaag
     5761 actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg tgcctggtcc
     5821 ttttttgctg ctgtcaaata gaaaatctta gagtatcact tcttctgcta ataactcagg
     5881 aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc catatggtgc
     5941 gtaagctttc tttttatctt tgccgacata gggagtttcc aagattttcg aaatattgtc
     6001 aaacattgga tcatggacga tcttattcaa cgtgtcgaat ccgattgttc caaaaccaat
     6061 attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga tatggagtac
     6121 tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg aaaagtcttc
     6181 tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg ataatttttc
     6241 attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac cgatttccgt
     6301 acctttacct gccatcgtct ccaaagcgat ttgtgccgtc tgttcttttg tcaaaacttc
     6361 attcaagcct tcaataattc tagcaattcc ggctgctgtt cctgccccta catgagcacc
     6421 tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct cttctctcag
     6481 aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat taatgatata
     6541 aggggcatga acgacgatat ttgttagacc gttttctgcc atgtacgctt ttccggcatc
     6601 aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg tatagatcat
     6661 aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca ttttctttcc
     6721 gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa ggcaattgtt
     6781 actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga tttttctttg
     6841 ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac tcttttataa
     6901 aaaagatcat tttctcacgt tttaactcct ttaaaaaaga aattcctgcc aaaaacagcc
     6961 ttgtctgttt ttttgttcta ttatttgcta taattagcga aataaaacca ttagttgagg
     7021 tgaccctttt gccacgcaaa aatactcgaa aaaaacagaa tagaagaaaa aaagaaaaat
     7081 ggtttgttcc taagattatt tttagagttt ttcaatcatt gactgttttt attactgttc
     7141 ttcttttgat gttcgcagct ctaggtattg gaataggcgc tggctatttt gcctatctcg
     7201 tcgaagacac acaattgccg actaaaaaag cactgcagac agaactagga aacattacag
     7261 aaacgtcaaa gattgtttat gcagataata cagagatatc aaaaatccag acagatctga
     7321 tgcgcacgac tatttcatct gataaaattt cacctttatt gaaaactgca attatcagca
     7381 cagaggatga atacttcgat aaacaccaag gttacgttcc taaagctgtt ttacgagcat
     7441 tagtatctga agctactggt attggctctt ctggtggatc aactttgaca cagcaattag
     7501 tcaaacagca gatccttaca gatgaaacaa cattcaaacg taaagcaaac gagatcattc
     7561 tttctgcaca agtagaaaaa tacttttcaa aagatgagat cattgctacg tatttgaatg
     7621 tctcaccatt tggtcgtaat aataagggac aaaacattgc tggagtccag gaagctgcaa
     7681 gaggaatctt tggtgtagat gcaaaagatg ttactctgcc gcaagctgct tatatcgctg
     7741 gattacccca aagcccgatc acgtatagtc catacacgaa cacaggtgcg ctaaaagaag
     7801 atctttctgc cggccttgcc agaaaagatt ttgttttatt cagtatgtat cgtgaaggcc
     7861 aaattacgaa agaacaatac gaagaagcaa aagcctatga ccttacaaaa gactttctac
     7921 cacaacaaat cgcagaacag actgacagag aattcttgta ttatactgtg atgaatgaag
     7981 caacaagaat cattgcacaa caattggctg aaaaagacaa cgctgatatg tctgattcat
     8041 cgatcagtga cgcttattat caaaaggcac agcaaacgat ccaaaataaa ggatatacaa
     8101 tccattcgac aatcgataaa gatgtctatg ctgcattgca aaatggcgtt gaaaattatg
     8161 gatacatgtt agatgatggt gctggcagtc aagtagaaac cggaaatgta ttgatggata
     8221 acagaactgg ccgcatctac ggattcgttg gcggacggaa ttacagtcag aatcaaaata
     8281 accatgcctt cgatacagaa cgtcaagctg gctcatcaat caaaccagtt cttgtttacg
     8341 gacctgcaat cgacatgggg cttatcggat cagaatcccg tgtatcagat tatgcaacaa
     8401 cttggcaaga aggagaaaat gctggcgaga aaatcgtcaa tgctactaac gaaggatcga
     8461 acaccttcca aaccgtccgt gagtcactag aatggtccaa caatatcccg gcttatcact
     8521 tatatcaaga tgtgttgaat tccggtggat cgaaacaata tgcttacgag cactacttgt
     8581 caaagatgaa ctacccggca aatgacaatt ggggcgttga atctgctcct ttaggtacag
     8641 tagatgtcac gacgcttcaa cagaccaacg gatttcaggc tttagcaaac ggcggcgtct
     8701 atgaagaagg ctatatcatc gattccatca cagataacgc aggaaatgta atttataaac
     8761 acgaaagcaa ctctgtccgg atctattcgg aagcaacggc atcgatcatg aatgacatga
     8821 tgcgcagtgt gatcaatgca aaaatcacta caccattcaa agatgccatc tcaagcttaa
     8881 acggaaatct aggaaaagca gattgggtcg gaaaaactgg ttctaccaat gaataccgag
     8941 attcatggct aattgtctct actccgtcta ttaccatcag tagctgggca gggcacgatg
     9001 acaatactgg aatggacagc aaagcaagga tacgttcggc taattatctt gccaatctga
     9061 tcaatcaagc ttaccaagca aaacctgaca tttttggcac tgatgagaaa ttcgaattat
     9121 catctgatgt gatgaagaaa aaagtatctg gatttactgg tcaaacacct ggaaaagtta
     9181 ctgtcaataa aaaatccatc caaacaccag gaaaaacagt tgaatcgcta tgggcgaaaa
     9241 atggaccgaa gaaaagcgcc tttaagtttg gcgttggcgg taccgatgaa aactatacag
     9301 attactggaa caccgcttca gcttatgcaa gagcgaatca agcaaccaaa gaatcagatg
     9361 agaaagatga ttgatttaat agaatacaca gtaataaggg aatgtgacaa tagtattgtc
     9421 acattccctt atttttttga tgatggtgaa caaaatacag catctataca cggattgttt
     9481 tatatacgat aaatataatt tcaaccactt tctctctttt ttaagacaca aaaaaaggat
     9541 tataacaaaa ttttgtcata accccttttc ccaaataaac ggtgttctcc tgccaactcc
     9601 gaatcacgag tcacaatatt catcaaacat gagatgctgt tttttgaaat tgctccttgt
     9661 aacgcagagt taaacggcag gcacacaacc tctgaatggt tgtcctttta ttattttgta
     9721 gatccttttt cttcgattcc ataaggcaag ataatcgttt tttcttcgac ttcttcttta
     9781 ttcatcattt tcgtcaatag gcgcatagat accgccccta aatcgtataa tggttgtgtg
     9841 atacttgtta aacgaggacg agctacttct gtcagtagtg aattgttact tgtgatgatt
     9901 tcgaattctt ccggtacttt cacgccacgg tctaataaac catccaaaat accgatggct
     9961 aattcatcat ctgaaacata gacagctgtt gcaccgctat tatggatccg atcgactaga
    10021 gatagaccat ctttgaagtt gtattgtgct tcaaagatca atccttcgct atatggtagg
    10081 ttattttcgc ttaatgcttc tttatagcct ttcagacgat tttgtccatt gatgggatcg
    10141 atcaacgcac cactgacaaa agcgattttt ttattgccgc tttttgccaa tttgtttact
    10201 gcatcttttg ttgcggatac ataatcaatg tttacgctac ctacttgttc atctggatca
    10261 atagagccag ctaatacaac aggagttttt gaacgtgaaa attctccacg gatttcgtct
    10321 gtgatatggt gacccatgaa gatcacgccg tccacttgct tagccagtaa gttattcaat
    10381 acattgactt ctttttggtc gttgccatct gaatttgcta agataatatt gtatttgtac
    10441 attgttgcca catcatcgat accacgtgct aaagaagcaa agaacatatt gctgacatca
    10501 gggatgatta caccaactgt tgttgttttc ttacttgcca agccacgcgc aactgcgttt
    10561 ggacgataat ccaagcgatc gataacttcc aagacttttt ttcttgttgc tggtttgacg
    10621 tttggattgc catttacgac acgtgaaaca gtagccatcg aaacatttgc ctctctcgct
    10681 acatcataga tagtgattgt ttgcttttcc atttgtgttc tcctattcat attttatatg
    10741 ttttctgaaa ata
//
LOCUS       DAJSNV010000089        10666 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSNV010000089 DAJSNV010000000
VERSION     DAJSNV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.092"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(183..1031)
                     /locus_tag="ON516_002233"
     CDS             complement(183..1031)
                     /locus_tag="ON516_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3345700.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1030..2097
                     /gene="gnd"
                     /locus_tag="ON516_002234"
     CDS             1030..2097
                     /gene="gnd"
                     /locus_tag="ON516_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR3345701.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="ON516_002235"
     CDS             2099..3100
                     /locus_tag="ON516_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR3345702.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="ON516_002236"
     CDS             3097..3381
                     /locus_tag="ON516_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3345703.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="ON516_002237"
     CDS             3414..4211
                     /locus_tag="ON516_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3345704.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="ON516_002238"
     CDS             4225..5058
                     /locus_tag="ON516_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345705.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="ON516_002239"
     CDS             5076..5933
                     /locus_tag="ON516_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3345706.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="ON516_002240"
     CDS             5955..6464
                     /locus_tag="ON516_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345707.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="ON516_002241"
     CDS             6501..6902
                     /locus_tag="ON516_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345708.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="ON516_002242"
     CDS             7014..7367
                     /locus_tag="ON516_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345709.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="ON516_002243"
     CDS             7401..7844
                     /locus_tag="ON516_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3345710.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="ON516_002244"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="ON516_002244"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3345711.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="ON516_002245"
     CDS             8939..9652
                     /locus_tag="ON516_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3345712.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9859..10008)
                     /locus_tag="ON516_002246"
     CDS             complement(9859..10008)
                     /locus_tag="ON516_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345713.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10404..10604
                     /locus_tag="ON516_002247"
     CDS             10404..10604
                     /locus_tag="ON516_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3345714.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3558 a         1691 c         2315 g         3102 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10261 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10321 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10381 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10441 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10501 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10561 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10621 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DAJSNV010000090        10661 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSNV010000090 DAJSNV010000000
VERSION     DAJSNV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.093"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            333..2279
                     /locus_tag="ON516_002248"
     CDS             333..2279
                     /locus_tag="ON516_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3345715.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="ON516_002249"
     CDS             2282..2737
                     /locus_tag="ON516_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3345716.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="ON516_002250"
     CDS             2751..4079
                     /locus_tag="ON516_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3345717.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="ON516_002251"
     CDS             4113..4397
                     /locus_tag="ON516_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345718.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="ON516_002252"
     CDS             4399..4914
                     /locus_tag="ON516_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR3345719.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="ON516_002253"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="ON516_002253"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3345720.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="ON516_002254"
     CDS             5599..6171
                     /locus_tag="ON516_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3345721.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="ON516_002255"
     CDS             complement(6358..7878)
                     /locus_tag="ON516_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3345722.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="ON516_002256"
     CDS             complement(8121..8306)
                     /locus_tag="ON516_002256"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR3345723.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="ON516_002257"
     CDS             complement(8290..8472)
                     /locus_tag="ON516_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345724.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="ON516_002258"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="ON516_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="ON516_002259"
     CDS             9426..10025
                     /locus_tag="ON516_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345725.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="ON516_002260"
     CDS             10089..>10661
                     /locus_tag="ON516_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HCR3345726.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DAJSNV010000091        10650 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSNV010000091 DAJSNV010000000
VERSION     DAJSNV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.094"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..656
                     /locus_tag="ON516_002261"
     CDS             33..656
                     /locus_tag="ON516_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345727.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="ON516_002262"
     CDS             1106..2155
                     /locus_tag="ON516_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3345728.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ON516_002263"
     CDS             2152..3033
                     /locus_tag="ON516_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3345729.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ON516_002264"
     CDS             3036..3806
                     /locus_tag="ON516_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3345730.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ON516_002265"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ON516_002265"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3345731.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ON516_002266"
     CDS             5117..6280
                     /locus_tag="ON516_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3345732.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ON516_002267"
     CDS             6397..6807
                     /locus_tag="ON516_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3345733.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ON516_002268"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ON516_002268"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3345734.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ON516_002269"
     CDS             9380..9664
                     /locus_tag="ON516_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345735.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ON516_002270"
     CDS             9824..10060
                     /locus_tag="ON516_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3345736.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ON516_002271"
     CDS             10057..10455
                     /locus_tag="ON516_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3345737.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2902 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt
//
LOCUS       DAJSNV010000092        10408 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSNV010000092 DAJSNV010000000
VERSION     DAJSNV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.095"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..672
                     /locus_tag="ON516_002272"
     CDS             <1..672
                     /locus_tag="ON516_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3345738.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
     gene            1194..1475
                     /locus_tag="ON516_002273"
     CDS             1194..1475
                     /locus_tag="ON516_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345739.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            1477..1893
                     /locus_tag="ON516_002274"
     CDS             1477..1893
                     /locus_tag="ON516_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345740.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            2515..3669
                     /locus_tag="ON516_002275"
     CDS             2515..3669
                     /locus_tag="ON516_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCR3345741.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3663..4085
                     /locus_tag="ON516_002276"
     CDS             3663..4085
                     /locus_tag="ON516_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345742.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4396..4776
                     /locus_tag="ON516_002277"
     CDS             4396..4776
                     /locus_tag="ON516_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3345743.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            4758..5672
                     /locus_tag="ON516_002278"
     CDS             4758..5672
                     /locus_tag="ON516_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3345744.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            5632..5910
                     /locus_tag="ON516_002279"
     CDS             5632..5910
                     /locus_tag="ON516_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345745.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            5917..6606
                     /locus_tag="ON516_002280"
     CDS             5917..6606
                     /locus_tag="ON516_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345746.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(6727..6813)
                     /locus_tag="ON516_002281"
     CDS             complement(6727..6813)
                     /locus_tag="ON516_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3345747.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            7450..8394
                     /locus_tag="ON516_002282"
     CDS             7450..8394
                     /locus_tag="ON516_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR3345748.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            8415..8948
                     /locus_tag="ON516_002283"
     CDS             8415..8948
                     /locus_tag="ON516_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3345749.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            8963..9133
                     /locus_tag="ON516_002284"
     CDS             8963..9133
                     /locus_tag="ON516_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345750.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            9588..9812
                     /locus_tag="ON516_002285"
     CDS             9588..9812
                     /locus_tag="ON516_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR3345751.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            9832..10119
                     /locus_tag="ON516_002286"
     CDS             9832..10119
                     /locus_tag="ON516_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3345752.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         3938 a         1754 c         1832 g         2884 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt agctaagtgg tgagtagttc acattaataa
      841 aaattgctgg ttttattctt ctttttgctt ttgcgagcag agcaacatgg acccatgttg
      901 cgatattttg gtggcgcccc aatccccgtt ttttcgttga atcttactcg gactgcactc
      961 gttttacagc tgatttttct gaattttgaa agagcataag gaaacgtggt atcactttct
     1021 tacgaaaggt gctagatagg ctgtgcctat cacgtttctc agaaaaaaat ttgctatcac
     1081 ttttttgcca ttgtggctag gctatgtata gtgttttggc gaaaaatagc tatacaaata
     1141 ctatatttgt atggtataca taaattatac gctgatacga aaggaaaatt tccatggaca
     1201 ttttagttcg aaatatcaat cccaaaatta ttgcaagaat agataaacgt tgtcaggaac
     1261 tcacaaccaa aacgggcaaa aaatggagtc gaaataaata tctaaaagtt ctaatcgaaa
     1321 gtgattttga ccgtcaactt atgaattaca aaaaagatca attcgattta ttactcgaaa
     1381 aatttattgc cattcaaaca gaaaatacga acatactgaa tgactatgtt cacacaaaca
     1441 accaactcat tgaaacactt atcggaggaa tataacatga gaaaaacttc tgttcaaaaa
     1501 cacgtaaccc tttatcccag tcacgttgac gttttagatt ccatcatcga aaaaaatcca
     1561 gacgtcaaca attattcaga agccattcgt tttttatgta tgagttatga acctgatacc
     1621 aatacaacca caaccagtaa ccaagcaaaa ctcaatgcca tgggaaaaga aatttctatc
     1681 atgacagaaa tcatggcaga atttgctgat ttatatctaa aagatactgg tgttcttatc
     1741 ggtacaaatt ctaaagtgta tcaagaagtg aaagaaaaaa ttgaaaatca actcaatcct
     1801 tcccgacgaa atcaacccaa aaatcgtcaa aaagaaacca atcaaacctt tacttctgga
     1861 aattttgacc caacaaactt tctaaaaaga taaaaaaaga aaccgatccc taaaaaaatt
     1921 cctcgatttc tttttaggga tcagtcttcc agcaagattt gcaagttggt accaacttgc
     1981 gaaaaaaaag aaaaatccgc ttatgcaact tcttcttttt gtattttgag gcgctacccc
     2041 aatccccaag tatcggttat ttaatagcgc ggaaatccaa agaataggac gtaccctgaa
     2101 acaaaaaaag actgctctca cgagcagccc aaaatgagat tatcctattc atcaacataa
     2161 aaatattaca aagtgaagaa ctagagaaag acaaaaaaag catatcaaat cctctcaaaa
     2221 ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaactaaaaa agagaaatcg
     2281 cgtaaaatca agcattagaa gctagtaact agctagacca attaaaaatc acaaagtgag
     2341 cgtctctatc cattggtaga ggggctactt ggcatagtgt acattatgtt aactaacgct
     2401 ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa atttgttatc
     2461 gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa aggcgtgatt
     2521 attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat gattggaatt
     2581 tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa tatcaactcc
     2641 caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca ctccataact
     2701 tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct tggttcgcca
     2761 tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc agtattttca
     2821 gacgctgata atacgattaa atatatttat accgtcaaaa aaaatacaaa tggagaatat
     2881 tcaggaagta tttcacaatt tttagcaaaa gaacttcaaa atctagtaga tagtaatata
     2941 acagatacaa ccttattttt tcaagataat agcatttatt attccgataa tggacaaatt
     3001 aaactacttt ggaaatcacc tttaagtaca gaaaaaaatg gtgtttcttc ctttacagaa
     3061 aaaaatgatc ttatatccgt aaatataaaa aataaaatta aaaatttctc taaaaaacaa
     3121 aattttcctg cggttaaacg tgtaaaaagt gtctcctccg gagataactt tattatgatt
     3181 aattggcgta tagcagaaac ccaaacaagt tttccgtggt gtgctgctta cgtagaagct
     3241 gctatcttat gtaacaaaac agatgtacgt gctacttcag ctagagccat tatgcattat
     3301 acctatccta atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca agatcaaata
     3361 ataaaatatg caaattactg tggtgtttat cctaaaaaag tagcacgaat actaaacctt
     3421 tcagaggtcc aaacacaact aagaaaagga aatgccattt atatgggaac tgctggttat
     3481 ggaaattata gtaacagccg tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt
     3541 tatcaaactt actatgtgtg gaatccttgg tataactacc caactgtaat aagtgcaaat
     3601 gcaggaccag ttacaattcc tgtacctgga ggtggtttta cttgggacaa taccattaca
     3661 aattggtaaa taaacccatt gctttagtta tgggtctagt gttagttctt ttcttaacta
     3721 gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat tccttttcta ggaacttggc
     3781 aaactaaaga tcattcagaa cgtctagtaa tcactaattt aaatacttca aaaaatatag
     3841 aacaatatac catcttagta aaagagaaca atcataaaat tcaaatcatt gataaaaatt
     3901 taaatataaa tgaacaagtt ttcatttaca aagcaacaaa taatttacta acactgcgct
     3961 ctaataaaaa acaattagat tacactttta gtttggattc tccaaaaaag ctcgtattta
     4021 ttttgacaac gaatgatggg acagaaggga cagctaaacc aattgaattt tttaaagctg
     4081 attgatgaat agttgtttga tattacaaaa caataatttc accgaaggtg aaacaagcga
     4141 aactttacaa aaagtggcta gcatttgcta gccttttttg tattgtggag cgtgggtttt
     4201 gagggggatt caagggggag cttgcgcccc tttgaaaagc ggttatttgc accaaatacg
     4261 taggtatttg gctagcaaat atagagcttg ccaagccctt tagtgagctt tagtgagctt
     4321 tacttattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     4381 gaaagggtgg gacttatgag cgaacaaaat caatatttgg ctagcaatca ttctaaaaaa
     4441 tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca
     4501 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
     4561 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgctca ggcaatcgcg
     4621 cgcgatttag ccaaagctgg cgggaatatc aatcaaattg ccaagtggtg caacacacac
     4681 caacacgacg ttgatcctgg cgacgcccaa cgcttatccg aaaacctaaa aatcatgcaa
     4741 aaggagctac aaaagatatg gcaacagtta aagtaagccg aaccacttct tgtagccgag
     4801 cgatcaatta cgccgaacca cgagcaaccg tcaaaacagg gataaactgt gacattgatt
     4861 atgccaaaag cgaaatgaaa caaattcgca tgctgtatgg aaaagacgat cgtgtacaag
     4921 cacacttatt gatccagtct tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
     4981 taggggcaga gtatgcgaaa aaaatagccc ctgaccacca aatcgcgatt tacacacata
     5041 cagacaaaga ccatattcat aaccacattg tcattaactc cgtcaatctg gaaactggga
     5101 acaaatttca agcccatggt caagcatttt taaacaaatg ttatgaccta aatgacgaga
     5161 tttgtcttga acatgactta acaatcactg aacgagggaa gaaacccgaa aaacgaacca
     5221 tgtctgaaat caaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa attcggtttg
     5281 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta taaccaattt tgtgattctc
     5341 ttaaaacatt cggcattcat tgcttgaatc gtgggaaaaa ttttacttac gaattgatcc
     5401 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
     5461 tacatgagct ggacagacga gaacgagcct acaatcaaca acgaaccaac gagctacgaa
     5521 gagctcgagc tagcattcgc gaaactgcaa aagaagtacg aagagaacaa cgaactctta
     5581 cgactgaaca accggaaatt agaccgactc gaagaaacca tgaccaacac gttgaaagag
     5641 gagcagacta ccaacaagaa ttatcgttct aaaacgttac aagacataga aaatgaaata
     5701 aagaaacaac gtgcttttct tgcaccttat accaaagaaa gaatacaaac tacaattgat
     5761 tcacaagtca aaaaattcca ccatgcgcta ttaaaccagc gaaataaaga acagaaaaaa
     5821 gtaattatgc agcgatcaat ggatattctt gcccccttat gttgcttaat catgacactg
     5881 ttagcctttt ggctggtatg gggagaataa atagaaatgg cattctattt accctatcta
     5941 ctcatttttg ccagtatttt aggaattatt tggcttagct ataaggctta tcaaacaaga
     6001 tattcattaa aaggctctaa aattcgattt aagaggtttt tattgccagg ggttatattt
     6061 acattatcaa tcgttttaag tggtttattc ggcgttttag agggaaataa acgtatttca
     6121 agaagtattt tattagggga tatcactcaa aagtatgaag cagcatgtga taaaaagaaa
     6181 aaggagcaag tgttaacaca aaaaatggaa gagtttacca cttgttatga ggaaatgaac
     6241 gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa actcactcgt
     6301 ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta tgagcgaacc
     6361 aaaaaagacg tgcaatattt taaagatacg cagacagaag aagcttgtta taatttattc
     6421 agtgatacaa tccctttttc aactagtgaa caggaacgaa aagaacggca acaatcagtt
     6481 acctacgaac gttataaagc actgcttcag caagcaacga acatacaaaa tccaacgaaa
     6541 aaagaaacgg cattaaatta tctaaaaagt gtcaaggaat ggttagatca gcaacagcaa
     6601 aattgaattt gatcggcaat catgttaaac taaacatgca aatgatcaaa cattagatac
     6661 acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat gtcgccttta
     6721 ttgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaacttc
     6781 tataagaagg gggcaaatga acaattcaaa cattagatac acctccttcc agtcgcaaga
     6841 ctgtcggata ggcgacaagt tattatatct taaaaaatta aacttgtgtg gtgtttttaa
     6901 gtatcgctta ttttagggtt gttagggctt gccctgaccg cctgtaaggc gctttaaagc
     6961 tagataagga tttttatcca agactgtctt aaaacggctt agataagcat tcagaaccgc
     7021 tcaaaggcct ctttaccaac atgtttttag caaaggagcc tttaggcgac tgcgcagtac
     7081 aatgtgagag cgtagcgatc acaccttttt ggcgaccgta ggaagcaagg ttttggattg
     7141 gcagttttga aaaatgcgcg aacctgttta aagaaaaaag aagtggtttt taggcgggaa
     7201 aattagcccc cctcttttta tatctctttt taaacctctt tttaaacctc tttaccgtaa
     7261 gctgtgtcct tactctacca atggatacag aatgttatga aggacatttt gtctgtttat
     7321 gaaggacatt ttgtctgttt atgaaggaca ttttgtctgt ttatgaagga cattttgtct
     7381 gtttgaacgt caaatatgaa tgtcttgaca tttaaaaaat gtcattcata attggattga
     7441 ggtgattata tggctaatga gattgtaaaa taccaccatg aattaaatac aattccttta
     7501 agaaaattta caccggttga aatgaatttg tttttttcca tcgtgtctag aatgcgtgaa
     7561 aaaggggata agacagttcg ttttacgttt gaccaactaa aagatttaag taactacaaa
     7621 gcaactgcaa atgtacgatt tgtagaagat ttaaaagcaa cttatgaaaa aatcttatct
     7681 ttacgctttg gtcgtagaag tgagtctggg ctttcttacg atatgtttgt aatgtttaca
     7741 gaatttaaaa tcaacgggga cgtagaaaat ccttacgtag atatacgaat ttacgaaaaa
     7801 gcactgcctt tgttaaataa tttagacgag tgggtacgct attctttaca acaattcaac
     7861 gagctacaaa gtggctatgc aaagactatg tttcgtctgt taaagcaata cagaaccaaa
     7921 ggtttcgcat atttctccaa agaagacttt cttgaattat tagatattcc taaaagttat
     7981 aagcaacctg atatagataa acgagttata aaacctatac gtcaagaact aactgcaatc
     8041 ttcaaaggac taacaatcaa aaagaaatac ggaaaaggac ggggaaaacc tgttattggc
     8101 tatcaattta cgttcaaacc tgagatcaaa aaagcagacg actttaacaa acatgcacca
     8161 accaaagaag accaaaataa agcattggct ttagctgata aacaaaaaga atacgaagaa
     8221 cccgctacaa caattaacga aaaaatcaaa cagaagaaac gagaacttga actcctccaa
     8281 caaatcaaac aactagaaaa agagaaaaaa gctcttgaaa aacaaacaga gtccgcactt
     8341 tcggaaaaag agaaaaaaag tttattatct gatttagata atttattcca gtagcaacct
     8401 aggaggcaac cagtatgcaa ccacaagaaa cctttacaat aaaggaattg tcagatcttt
     8461 ttaagatgag taggcaagca atatcgaaac atatccaaaa attagattct tccatgatag
     8521 ctaaaaatga gcgtggttat aaggttgtat tgcgttctgg agtattgcaa cttgctcgca
     8581 acttagacaa tcaaaggttg ctagataagt tgcataaaca tgaacaaaac aacgataaaa
     8641 atgcttatat accaaacaaa aacgtggttg caaacaagtt gctagatcag ttgcgtgaag
     8701 aaaatgaatt tttacgagaa caacttgccc tcaaagagca aaataattct catatacaaa
     8761 aactactcga ccagcaacaa caactcaccc tgcaagccaa ccaacaaatc gaaaaacttc
     8821 aggagcaact gcagttgact tatacggaag agtcttcaga gaatagttca acgactcttt
     8881 ctgaagcaac agaaacaatg gacgaacaaa caaagaattg gaaaaaatgg tggcagctat
     8941 ggaaatgaag aaggaaatac ttatgacaaa aaaacacttg ctattcggtt ctatcctact
     9001 aggaatatta agctttagcc caatgttatt atcagtggcc aacaaaggaa ataaaaaagg
     9061 ggaactaaaa ctcaatccta attactacaa cagtcaaaat gaggaagtat ttgtagaaaa
     9121 acttgataaa taagaaaaga ctgctcgtac gagcagtctt tctgaaagag attatcctat
     9181 tattaacctt agaaaagaat aataaatgaa tatgagatat gtaaaaagca taacaaacac
     9241 aaacaaaaat aacaagtatt taaaaaatga ccgatcaata ttcacaaaga attagaaaga
     9301 aacaaccgca gaaaaccaag ccttaaaaag agcttttaac ctagaccaat aaaaattcac
     9361 aaaatcagcg cctggatccc ttggtacagg cgttacttgg tagtatacac attatgtcta
     9421 ctagctgttt aaataactga tcttataatt ttatttaaac gattatgaat tcaaaactac
     9481 tttttatgac gttaatcatt gttatataat aaaatgttat ataataaaat tactaagatt
     9541 atttaatata acaagcaatt ttaaaaaaac gaaaaggaaa aacaatcatg aaaaagaaag
     9601 tattaaaaca ttgtgttatt ctaggaatat taggaacttg tctagctggc atcggtacag
     9661 gaataaaagt tgatgcagct acttactatg gaaatggtct ttattgtaac aaagaaaaat
     9721 gttgggtaga ttggaatcaa gctaaaggag aaattggaaa aattattgtt aatggttggg
     9781 ttaatcatgg tccatgggca cctagaaggt agtggtaggg aggttattat aatggatttt
     9841 actaaagaag aaaaactttt aaatgcaatt agtaaagtat acaatgaagc aactatagat
     9901 gactatcctg acttaaaaga aaagctcttt ctttattcta aagaaatcag tgagggaaaa
     9961 agtgttggtg aagttagtat gaaattaagt agttttcttg gaagatatat tttaaaacat
    10021 aaatttggat tacctaaatc tttaatagaa ttacaagaaa ttgttagtaa ggaatctcaa
    10081 gtatatagag gatgggcttc tattggtatt tggagttaat tctttggaat aaaatgaaaa
    10141 tgggactgaa gaatcaattc tgattcatca gtcccataaa ttatagagcc gaaaaattat
    10201 catatttata gatttattaa tagtaaatgg cataagaatc tttgaacaac gaaaaaaatg
    10261 tagctctacc taattattga tttgcaacaa tttcaccgaa ggtgaaacaa gcgaaacttt
    10321 acaaaaagtg gctagcattt gctagtcttt tttgtattgt ggagagtggg ggggaggggg
    10381 attcaagggg gagcttgcgc ccctttga
//
LOCUS       DAJSNV010000093        10135 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSNV010000093 DAJSNV010000000
VERSION     DAJSNV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.096"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            119..625
                     /locus_tag="ON516_002287"
     CDS             119..625
                     /locus_tag="ON516_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3345753.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ON516_002288"
     CDS             642..1541
                     /locus_tag="ON516_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3345754.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ON516_002289"
     CDS             1628..2512
                     /locus_tag="ON516_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3345755.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ON516_002290"
     tRNA            complement(2704..2775)
                     /locus_tag="ON516_002290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ON516_002291"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ON516_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3345756.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ON516_002292"
     CDS             4318..5409
                     /locus_tag="ON516_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3345757.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ON516_002293"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ON516_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3345758.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="ON516_002294"
     CDS             6561..7751
                     /locus_tag="ON516_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3345759.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="ON516_002295"
     CDS             7855..8610
                     /locus_tag="ON516_002295"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3345760.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ON516_002296"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ON516_002296"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3345761.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2100 g         2618 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagctgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggtggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 tttttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga gtttt
//
LOCUS       DAJSNV010000094        10056 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSNV010000094 DAJSNV010000000
VERSION     DAJSNV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.097"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            222..491
                     /gene="rpsN"
                     /locus_tag="ON516_002297"
     CDS             222..491
                     /gene="rpsN"
                     /locus_tag="ON516_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3345762.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            506..685
                     /gene="rpmF"
                     /locus_tag="ON516_002298"
     CDS             506..685
                     /gene="rpmF"
                     /locus_tag="ON516_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3345763.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            717..1094
                     /locus_tag="ON516_002299"
     CDS             717..1094
                     /locus_tag="ON516_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345764.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1115..1264
                     /gene="rpmG"
                     /locus_tag="ON516_002300"
     CDS             1115..1264
                     /gene="rpmG"
                     /locus_tag="ON516_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3345765.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1288..2271
                     /locus_tag="ON516_002301"
     CDS             1288..2271
                     /locus_tag="ON516_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3345766.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2301..2423
                     /locus_tag="ON516_002302"
     CDS             2301..2423
                     /locus_tag="ON516_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3345767.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2441..3964
                     /locus_tag="ON516_002303"
     CDS             2441..3964
                     /locus_tag="ON516_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3345768.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3988..4227
                     /locus_tag="ON516_002304"
     CDS             3988..4227
                     /locus_tag="ON516_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345769.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4420..4881)
                     /locus_tag="ON516_002305"
     CDS             complement(4420..4881)
                     /locus_tag="ON516_002305"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3345770.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5076..5294)
                     /locus_tag="ON516_002306"
     CDS             complement(5076..5294)
                     /locus_tag="ON516_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345771.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5412..7028
                     /locus_tag="ON516_002307"
     CDS             5412..7028
                     /locus_tag="ON516_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3345772.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7269..8609)
                     /locus_tag="ON516_002308"
                     /pseudo
     CDS             complement(7269..8609)
                     /locus_tag="ON516_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8885..9040)
                     /locus_tag="ON516_002309"
     CDS             complement(8885..9040)
                     /locus_tag="ON516_002309"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3345773.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9336..9668)
                     /locus_tag="ON516_002310"
     CDS             complement(9336..9668)
                     /locus_tag="ON516_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3345774.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3341 a         1600 c         1988 g         3127 t
ORIGIN      
        1 aattcttatt tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg
       61 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
      121 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
      181 tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
      241 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
      301 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
      361 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
      421 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
      481 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
      541 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
      601 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
      661 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
      721 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
      781 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
      841 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
      901 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
      961 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
     1021 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
     1081 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
     1141 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
     1201 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa
     1261 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
     1321 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
     1381 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
     1441 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
     1501 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
     1561 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
     1621 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
     1681 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
     1741 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
     1801 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
     1861 acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa
     1921 agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat
     1981 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttactccaa
     2041 tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
     2101 gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt
     2161 caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat
     2221 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
     2281 gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
     2341 aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa
     2401 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
     2461 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
     2521 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
     2581 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
     2641 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
     2701 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
     2761 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
     2821 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
     2881 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
     2941 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
     3001 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
     3061 gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc
     3121 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
     3181 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa
     3241 aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac
     3301 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
     3361 gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
     3421 tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag
     3481 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
     3541 agttttcgat tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta
     3601 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
     3661 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
     3721 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
     3781 tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca
     3841 tttccatatt ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg
     3901 gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca
     3961 ttaaaattca atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
     4021 aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt
     4081 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
     4141 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
     4201 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
     4261 tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat
     4321 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
     4381 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
     4441 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
     4501 atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa
     4561 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
     4621 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
     4681 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc
     4741 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc
     4801 atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct
     4861 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
     4921 tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc
     4981 catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa
     5041 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
     5101 ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt
     5161 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
     5221 atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga
     5281 cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
     5341 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
     5401 agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa
     5461 aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg
     5521 taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa
     5581 gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag
     5641 atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg
     5701 aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc
     5761 ctttagacat tgatttgacc caacgattag agattggtgc cattattata gtagtggatg
     5821 taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag
     5881 gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt
     5941 gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta
     6001 acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag
     6061 tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag
     6121 tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat
     6181 atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc
     6241 atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg
     6301 cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat
     6361 cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa
     6421 atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt
     6481 ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg
     6541 attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata
     6601 tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg
     6661 tcataattat tggcattttg attattacct cttttattcc cttatttgta ttctctatag
     6721 aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca
     6781 aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg
     6841 cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata
     6901 atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta
     6961 tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca
     7021 tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata
     7081 atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca
     7141 acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac
     7201 ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac
     7261 tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg
     7321 gtagtattct tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag
     7381 ttttgaaagt ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa
     7441 cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat
     7501 ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt
     7561 aggagcgcga cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag
     7621 acttcgtagc ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct
     7681 tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac
     7741 gcattctttt gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt
     7801 ttaaaggtct tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta
     7861 atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga
     7921 tagggtgtat agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag
     7981 cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga
     8041 tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg
     8101 aattcatcga aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga
     8161 aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa
     8221 gacatagaaa gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg
     8281 gagcagcgac gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat
     8341 ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag
     8401 atggatactt ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga
     8461 aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg
     8521 attcgctctt tgcgaaaggc atcttcatga aagataagat ttaggtctag aatatctagt
     8581 aactctttgg taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg
     8641 actttattct aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat
     8701 tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa
     8761 attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta
     8821 aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta
     8881 attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag
     8941 tttggctcac tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc
     9001 gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta
     9061 atggacggac gacaatctct tttttcatgt gtgaatctac catatagtaa cgtagaatac
     9121 gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag
     9181 taatatccat cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt
     9241 ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc
     9301 tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat
     9361 tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta
     9421 gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct
     9481 ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct
     9541 gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta
     9601 gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt
     9661 tttctcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat
     9721 agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt
     9781 taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag
     9841 ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga
     9901 agtcctcgtt gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt
     9961 aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat
    10021 taaaaaacgt tgataattaa aatcattcgt tttaga
//
LOCUS       DAJSNV010000095         9915 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSNV010000095 DAJSNV010000000
VERSION     DAJSNV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.098"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(378..602)
                     /locus_tag="ON516_002311"
     CDS             complement(378..602)
                     /locus_tag="ON516_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345775.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            793..3570
                     /locus_tag="ON516_002312"
     CDS             793..3570
                     /locus_tag="ON516_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3345776.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3652..4071
                     /locus_tag="ON516_002313"
     CDS             3652..4071
                     /locus_tag="ON516_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3345777.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4083..4553
                     /locus_tag="ON516_002314"
     CDS             4083..4553
                     /locus_tag="ON516_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3345778.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4570..5349
                     /locus_tag="ON516_002315"
     CDS             4570..5349
                     /locus_tag="ON516_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3345779.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5336..6157
                     /locus_tag="ON516_002316"
     CDS             5336..6157
                     /locus_tag="ON516_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3345780.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6174..7223
                     /locus_tag="ON516_002317"
     CDS             6174..7223
                     /locus_tag="ON516_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3345781.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7236..8240
                     /locus_tag="ON516_002318"
     CDS             7236..8240
                     /locus_tag="ON516_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3345782.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8873..9892
                     /locus_tag="ON516_002319"
     CDS             8873..9892
                     /locus_tag="ON516_002319"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3345783.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3322 a         1644 c         2074 g         2875 t
ORIGIN      
        1 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
       61 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct
      121 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      181 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      241 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      301 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      361 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      421 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      481 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      541 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      601 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      661 aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat
      721 aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat
      781 gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag
      841 acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat
      901 cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa
      961 ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa
     1021 caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag
     1081 ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg
     1141 aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt
     1201 ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat
     1261 tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa
     1321 tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac
     1381 actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt
     1441 ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat
     1501 caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc
     1561 ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata
     1621 cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg
     1681 atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga
     1741 ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat
     1801 atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat
     1861 gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca
     1921 cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag
     1981 cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat
     2041 aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat
     2101 tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa
     2161 tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac
     2221 agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa
     2281 gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca
     2341 tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa
     2401 gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa
     2461 gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg
     2521 gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta
     2581 gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt
     2641 tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt
     2701 aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc
     2761 acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt
     2821 cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc
     2881 attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa
     2941 agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat
     3001 aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca
     3061 gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag
     3121 atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc
     3181 aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta
     3241 gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg
     3301 aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt
     3361 gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag
     3421 tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat
     3481 agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca
     3541 gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg
     3601 catgcaactt gcttttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga
     3661 gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg
     3721 atctatggga aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat
     3781 ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg
     3841 acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat
     3901 gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac
     3961 gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc
     4021 cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa
     4081 aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat
     4141 ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg
     4201 agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat
     4261 caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca
     4321 tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa
     4381 ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa
     4441 atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag
     4501 aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga
     4561 agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca
     4621 gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga
     4681 atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt
     4741 atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta
     4801 gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg
     4861 ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt
     4921 atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg
     4981 cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat
     5041 atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag
     5101 gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg
     5161 ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta
     5221 gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat
     5281 attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa
     5341 gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg
     5401 ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta
     5461 tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt
     5521 gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat
     5581 cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga
     5641 tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg
     5701 aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt
     5761 aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc
     5821 aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat
     5881 gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac
     5941 tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac
     6001 tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat
     6061 agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt
     6121 ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta
     6181 cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg
     6241 atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt
     6301 catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg
     6361 tcgatattca agaacctttt tattttttca attatggaaa actttctcct tcaatcgata
     6421 ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga
     6481 tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt
     6541 tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa
     6601 aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta
     6661 aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca
     6721 accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat
     6781 ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag
     6841 aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag
     6901 cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag
     6961 gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg
     7021 gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa
     7081 tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt
     7141 ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata
     7201 cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac
     7261 aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata
     7321 aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt
     7381 cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc
     7441 atgctgcaga atattacact actggaaaca aaagattaac aaaagattct atcgttattt
     7501 tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag
     7561 taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg
     7621 attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag
     7681 atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg
     7741 aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat
     7801 catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat
     7861 ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga
     7921 aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc
     7981 cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac
     8041 ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag
     8101 gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac
     8161 aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact
     8221 atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca
     8281 gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat
     8341 tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc
     8401 attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag
     8461 tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa
     8521 gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga
     8581 aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac
     8641 ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa
     8701 agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag
     8761 taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta
     8821 agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca
     8881 aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga
     8941 aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa
     9001 gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata
     9061 taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa
     9121 agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat
     9181 acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga
     9241 agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga
     9301 ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc
     9361 agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt
     9421 gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat
     9481 ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga
     9541 aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg
     9601 tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa
     9661 cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga
     9721 gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc
     9781 aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa
     9841 agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga
     9901 tcaatagtta atcgt
//
LOCUS       DAJSNV010000096         9832 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSNV010000096 DAJSNV010000000
VERSION     DAJSNV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.100"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            510..1073
                     /locus_tag="ON516_002320"
     CDS             510..1073
                     /locus_tag="ON516_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345784.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1134..1499)
                     /locus_tag="ON516_002321"
     CDS             complement(1134..1499)
                     /locus_tag="ON516_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345785.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1575..1820)
                     /locus_tag="ON516_002322"
     CDS             complement(1575..1820)
                     /locus_tag="ON516_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3345786.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1890..2144)
                     /locus_tag="ON516_002323"
     CDS             complement(1890..2144)
                     /locus_tag="ON516_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345787.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2308..2631)
                     /locus_tag="ON516_002324"
     CDS             complement(2308..2631)
                     /locus_tag="ON516_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345788.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2818..2891
                     /locus_tag="ON516_002325"
     tRNA            2818..2891
                     /locus_tag="ON516_002325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Ile,seq:cat)
     gene            complement(3276..3773)
                     /locus_tag="ON516_002326"
     CDS             complement(3276..3773)
                     /locus_tag="ON516_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3345789.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3813..4622)
                     /locus_tag="ON516_002327"
     CDS             complement(3813..4622)
                     /locus_tag="ON516_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3345790.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4956..5813
                     /locus_tag="ON516_002328"
     CDS             4956..5813
                     /locus_tag="ON516_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3345791.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5806..6708
                     /locus_tag="ON516_002329"
     CDS             5806..6708
                     /locus_tag="ON516_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3345792.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6741..7091
                     /locus_tag="ON516_002330"
     CDS             6741..7091
                     /locus_tag="ON516_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3345793.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7140..8063
                     /locus_tag="ON516_002331"
     CDS             7140..8063
                     /locus_tag="ON516_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3345794.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8141..9034
                     /locus_tag="ON516_002332"
     CDS             8141..9034
                     /locus_tag="ON516_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3345795.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9072..9635
                     /gene="amaP"
                     /locus_tag="ON516_002333"
     CDS             9072..9635
                     /gene="amaP"
                     /locus_tag="ON516_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3345796.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9649..>9832
                     /locus_tag="ON516_002334"
     CDS             9649..>9832
                     /locus_tag="ON516_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3345797.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFK"
BASE COUNT         3421 a         1663 c         1849 g         2899 t
ORIGIN      
        1 attcattggt cgttgaatgg gctgaatagt agtgtataaa acaattttgt ggagcgtgaa
       61 gcttttatgg tagaggaaaa tacgaataaa gtgatgttga taaaaagatt cctatcttga
      121 atatgaatcg agatgtcttg aaaatgaatg agtttgacca atcattatcc tatcgaaatt
      181 tacagtttcg ttcgattggt ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg
      241 aagagggcgg cacatctaaa tttttaagta acggacagtt caaatatcaa gcattgaaag
      301 aggctatcct actgtttgaa ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag
      361 ttcattaaat cccttcaagt gatgaaagaa tgagatctaa taaaacctga tctttcatct
      421 gtttttttaa taacgtaata atttttttat tttttgtgct ttcgccgtta ttgtagagtg
      481 atttagcatt cttattttta aaaataactg tgttatatat taaaaaaggc ataatagtta
      541 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
      601 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      661 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      721 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      781 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      841 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      901 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      961 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
     1021 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
     1081 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
     1141 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
     1201 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
     1261 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
     1321 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
     1381 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
     1441 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
     1501 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1561 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1621 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1681 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1741 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1801 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1861 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1921 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1981 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     2041 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     2101 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     2161 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     2221 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     2281 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     2341 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     2401 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     2461 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     2521 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2581 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2641 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2701 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2761 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
     2821 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     2881 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2941 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
     3001 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
     3061 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
     3121 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
     3181 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
     3241 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
     3301 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
     3361 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
     3421 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
     3481 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
     3541 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
     3601 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
     3661 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
     3721 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
     3781 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
     3841 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
     3901 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
     3961 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
     4021 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
     4081 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
     4141 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
     4201 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
     4261 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
     4321 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
     4381 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
     4441 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
     4501 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
     4561 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
     4621 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
     4681 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
     4741 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
     4801 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
     4861 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
     4921 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
     4981 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
     5041 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
     5101 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
     5161 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
     5221 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
     5281 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
     5341 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
     5401 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
     5461 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
     5521 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
     5581 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
     5641 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
     5701 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
     5761 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
     5821 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
     5881 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
     5941 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
     6001 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
     6061 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
     6121 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
     6181 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
     6241 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
     6301 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
     6361 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
     6421 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
     6481 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
     6541 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
     6601 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
     6661 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagacaaca
     6721 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
     6781 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
     6841 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
     6901 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
     6961 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
     7021 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
     7081 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
     7141 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
     7201 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
     7261 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
     7321 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
     7381 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
     7441 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
     7501 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
     7561 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
     7621 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
     7681 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
     7741 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
     7801 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
     7861 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
     7921 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
     7981 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
     8041 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
     8101 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
     8161 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
     8221 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
     8281 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
     8341 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
     8401 aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga ttgtcttact
     8461 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
     8521 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
     8581 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
     8641 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
     8701 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
     8761 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
     8821 tggtgtagca cctggtccaa tctggacacc attacaacta gacgaaggac aaccttctga
     8881 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
     8941 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
     9001 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
     9061 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
     9121 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
     9181 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
     9241 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
     9301 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
     9361 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
     9421 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
     9481 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
     9541 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
     9601 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
     9661 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
     9721 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
     9781 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa at
//
LOCUS       DAJSNV010000097         9821 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSNV010000097 DAJSNV010000000
VERSION     DAJSNV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.101"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            205..684
                     /locus_tag="ON516_002335"
     CDS             205..684
                     /locus_tag="ON516_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345798.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ON516_002336"
     CDS             717..2945
                     /locus_tag="ON516_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3345799.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ON516_002337"
     CDS             3074..4717
                     /locus_tag="ON516_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3345800.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ON516_002338"
     CDS             complement(4790..5335)
                     /locus_tag="ON516_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3345801.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ON516_002339"
     CDS             complement(5358..5843)
                     /locus_tag="ON516_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3345802.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ON516_002340"
     CDS             complement(5833..6645)
                     /locus_tag="ON516_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3345803.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ON516_002341"
     CDS             complement(6784..8316)
                     /locus_tag="ON516_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3345804.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ON516_002342"
     CDS             complement(8425..9456)
                     /locus_tag="ON516_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345805.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3267 a         1828 c         1890 g         2836 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa a
//
LOCUS       DAJSNV010000098         9761 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSNV010000098 DAJSNV010000000
VERSION     DAJSNV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.102"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            117..311
                     /locus_tag="ON516_002343"
     CDS             117..311
                     /locus_tag="ON516_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3345806.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            314..1516
                     /locus_tag="ON516_002344"
     CDS             314..1516
                     /locus_tag="ON516_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3345807.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2099..3730)
                     /locus_tag="ON516_002345"
     CDS             complement(2099..3730)
                     /locus_tag="ON516_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3345808.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3744..5417)
                     /locus_tag="ON516_002346"
     CDS             complement(3744..5417)
                     /locus_tag="ON516_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3345809.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5430..5747)
                     /locus_tag="ON516_002347"
     CDS             complement(5430..5747)
                     /locus_tag="ON516_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3345810.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5772..7172)
                     /gene="lacG"
                     /locus_tag="ON516_002348"
     CDS             complement(5772..7172)
                     /gene="lacG"
                     /locus_tag="ON516_002348"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3345811.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7409..8194
                     /locus_tag="ON516_002349"
     CDS             7409..8194
                     /locus_tag="ON516_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3345812.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8197..8889
                     /locus_tag="ON516_002350"
     CDS             8197..8889
                     /locus_tag="ON516_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3345813.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9163..9534)
                     /locus_tag="ON516_002351"
     CDS             complement(9163..9534)
                     /locus_tag="ON516_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3345814.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3097 a         2064 c         1706 g         2894 t
ORIGIN      
        1 gttgggaagt cctgaacaag tgatggattt tcaagaaagt accgttcatt atacaaaaat
       61 aaagaaataa cactattacc caatgatagt gttttctttg aaagtgagtt tatacaatga
      121 aaaatacaat ttgtcgaaaa gatttagagg ctctaggatt taaaacacat caagctacca
      181 caataatccg acaagcaaaa acaagaatgg taaatcaggg atatccatat tataataata
      241 aaagagttgg ggtagtaccc cgacaagtaa ttgaagaaat tataggtgtt cctctttcac
      301 aggaggaata actatgaaaa caaaataccc ttgtgtgtat caagataaaa atggtcgctt
      361 ttcttatcta gttgagttag gaacagatca agtgactgga aagaggatcc agaagaaagg
      421 aacaaaagat tctctaggaa aacgattctc atcagctcgt gaagcacaca aagaagtcat
      481 gcgtataaaa aatgaataca tggttgtaaa tggatttgcg aattatgatt taacttacaa
      541 agaatttatg gagcagtctt acgttccaca ctacaaagct agcgtacaaa aaagtacttg
      601 gagttctcgt gagtgtggat tgattcagat tactgaattt ttcggtgata agaagcttcg
      661 tgaaatcaat gtgcgagatt gtgaaaatta tcgaatttgg ttattgaacg atagcggata
      721 ctcacaagca tacagttcac ttatgtacgg tatgtttcgt aaatcattag actatgcggt
      781 caccctgcaa tttctgaatg agaatatttc caaaagaaca aaagcaattc caaaaggaaa
      841 atcaatcgtt ccttactgga caaaagaaga atttgaagaa gttttggcaa aaatttataa
      901 agatgatttc tacgaacata tgtgtttttt aatgatctgg ctatattaca tgactggtgt
      961 tcgtgtaagc gaaggattag cacttaattg gaatgacgtg gacttcaaac gtaaaaagtt
     1021 gagggttcat cacaccttgg aaatgacaaa tcaaaaagat tttattcgga aaccttacac
     1081 aaaaactgaa agcggcttgc ggatcatttc tctggacgat gatacaattg aagtgctaaa
     1141 aaagtggaaa gttattcaaa aaaatcatgg tgtagaacaa tttattctta gctacactga
     1201 cctcccactc tatcgttcaa ctgttcaacg aattattcag cgttatgcca aactagctaa
     1261 ggtaccccta atacaaggaa aaggattacg tcattctcat gttagctacc taatcaatga
     1321 gtttaacgca gacattctgg tcgtgtcgca acggttaggc cattctagcc ctgaaatcac
     1381 tttaaagcac tacgctcatc tatggagccg taatgatgaa ggaattgcag aacaaatgac
     1441 aggaaacatt aaatttacct gtgcaacaaa atctaaagtt gcaaacttta atggaaatca
     1501 agcagtaaaa atctaaccct accatttccc taccaaaatg aggaataggc tgaaaaagca
     1561 ctgatataat agcttcttta cgaggctact catattgagt gggaataaag agccaacata
     1621 atatggaggg ggaatcccta taaatcaaca ttcttcaata gctaaaatgc ttgattttca
     1681 ttaaaaataa gatggttccc taccaaaaga gactataata taagttaatt tgtttcattc
     1741 ttatcataaa aagaagttct cgaaaaacga actgttttcc gagaactttt ttctatccat
     1801 ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt accctttctt aaacaaactt
     1861 tatgttatct gcacaacttt ttgcaatttt ttcattgctt cttacgaaat cgttgaactc
     1921 tctttagcat aatacaaagt gtagatgaag tctggtgctt tactcgtttt ggtaaaaatg
     1981 actggaaatc gtttctggtt atttttggca aaggcgaggt gcaggttcga ttcctgcctt
     2041 ctacgctaac gatgtggctg aagggaacct ccgatcttga atcgaaacga tccgcagtct
     2101 attgtttcac caatgaaaca aaatcaacgt tcgtccaagc atttcctgaa gccttgctat
     2161 aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc tttgattgta atttttgtcc
     2221 attcacttgt tttaacaacc gcagtattta gttccgctcc accataattt ttgatatata
     2281 actgattagt atcaaactct cccccagaca ttacataggc actcaatgtg taagttcctt
     2341 tgcttatgcc actaacattt tgataagctg atgcctcgta tgcttgatcc tgccaaaaag
     2401 ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt gatcgtaccc ttagcggttc
     2461 cttgttgaaa ccaggtttgc caattggaag gactggtcgt tggtccatcg ctttcgaaac
     2521 ttgcattttt aactagattt atttgcgccc ctgcttgttt aaacgcacgc aagcttggca
     2581 aggcgtttcc agcaaaatca aacatcgctt ggttgtccca ttcgtttccc atagtggatt
     2641 gaacactgag ataattcata cctgcttgtg ttgcccaact cacatttccg ttgtaccaaa
     2701 gaggttccca atagaagaag cccgccccgc gattattcgg tactgcctga atccgatcgg
     2761 caagatccag caaatactct gcttgtcctg tcggtgttgg ttcatatccc cccgcagctg
     2821 cttcggcttc accaaaagca tttggcatag tatcggcatt cgctgttgta tgcccataag
     2881 ctgtttcaac tacgatcact tctttaccat accgttggct gatcgcattc atattggttt
     2941 gaagttcatt cattgaaccg tgccaatacg gataatacga cactccaatt aaatcaaatg
     3001 acacacctcg actcgtgatt tcatcaaacc accaagtaaa tgctcctgta tcgccaccat
     3061 cagctaagtg gagcattact ggagttgttt taggttgagt gtctcgaaca gcttggatac
     3121 cagacttcaa aaaagcagcc aatcgatcaa attctccccc gccttctccc caagacttgc
     3181 cataaggcca gagcattccg ctattcagtt cattgccgat ctgaaccata tcaggataaa
     3241 cgtccagctg cttcatttcc gacagaacgt tctttgtata gtcgtacaat gccgtattca
     3301 tttcttcaaa ggtcagacct tgccatgctt taggaagatt ctgtttccca ggatcaaccc
     3361 aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc cagatttttt gctcgttttg
     3421 caaggtcaat cgtcgtttcc agatcatttg tccctgctcc ataactttct ccattactat
     3481 tataaggatc ctgccacaaa cgcagtcgaa catagttgac accattattt tttaaaatct
     3541 ctaaggcatc ttttgaaata tcgtcttcat aatattttgc accagatttt tccatatctg
     3601 acaggatcga aatatctgct ccgcgaataa attcgctggc agatgcttca acagtttttg
     3661 aaacagcccc caaagcccct aacacagctg caaataaaac agctttacgc aaccaaattc
     3721 ccattttcat tatggctcct cctttattga aattggttca gaataaactg aacagctttt
     3781 tcaggatctc tcgttaatcc gatatattct gctccttttg tcgcaaccag cttgattcct
     3841 aagcgatcgg tgtcttcttt gatatcatca tagtaagaat tgacttgcgg tgctaagacc
     3901 accatatcaa aatcctgcag catttcgtaa tgagaaccat aagcacccgc cattgcactg
     3961 atcggtgctt tcgattcaga tgcacctttt tttaaagcat tagctaacat cgcacttgtc
     4021 cctgcgccag cacaaagtac caacacattg atttctttat ttgcatcgct gataacttga
     4081 ctcgctgcca caggtacttc ctcacgtaca tcggcagaag caccttcctc ggttgcgatc
     4141 acctctgctt tttgtgcttc tttctctact aagacagcat cgtatgcttt gcaaaatggt
     4201 agatagatca ggaagtcaac gacgattaga accgctgcta gaacaaaagc taagataccc
     4261 atgcccgttc ctagaaccaa accgatcggt gctggagttg cccaaggcaa aacgtacata
     4321 aagctgttca tgcccaatac atcgacaaaa aacttgaaca ggcaaacgtt tacaacaggt
     4381 gttaacaaaa atggaataaa aaaataaggg ttcaaaatca tgggtgccgc aaatagcaat
     4441 ggttcgttga cagcgaaact tactggaata aaggaagctt tcccaactgc cttcaactgc
     4501 ttagacttag caaagagtag gaagataaaa ggaacaacca atgttgcccc agttcctccc
     4561 atagttccta caaaattccc taaacccacc gtcagtactt tagaagcttg ttcaccggaa
     4621 ttgaacaatt gcagattggt ttccacattg gcatagataa ttgccgcaat tgccggctcc
     4681 acgatcgacg gtccatgaac accgacaaac caaaacagcg ccatcgctcc ccagataata
     4741 gcgattccta agtacccgtc agctgctgta aatagcggct gcagcaccgt aatgattgct
     4801 tctgcaaatg agatttggaa gatttgtcga atgcctaaat caattaaaac ggccgcaaat
     4861 actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg aaatcgttcc aggaacttct
     4921 ttaggcattt taatggtaat atctttcagc acacaaaact tatataaatt aactgttaaa
     4981 aatgcagcga caaaggaagc cagtagtccc tttgtcccca tatattccgt tgaaaatcca
     5041 ccttcaatcg gagagacgct taacaacagg aaacttacca cagaggccag catcacagag
     5101 gtggtgttga ttactttgtt tttcggtaag cgacgattca tcgaatcagc caagcagcga
     5161 gcagtggttg ctgaaacgag taaccctaca acacccatcg aataattata gactttccat
     5221 agccatgtgg agacagattc aggccattgg taaccaaaaa tctcaggaac cgcagcaatc
     5281 aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg ttaaaaatcc gtcacgaacg
     5341 gctcctaaat aactattacg tgagattttt tcaaaaaatg gtttcccttt ttcgatttgt
     5401 ttaatgattc catccatagc gttcttctcc tatccttgat tctgtctgta cagtacaata
     5461 aagtcttgaa tcagatctct cagcaataaa gtggtcatta aatgatcctg cccatggata
     5521 aaaatgaatc ccatctccat ctctttccca gatgcttctt ccgctaaaat ttttgtttgc
     5581 gaattatgtg caagggttaa attttcgtcg gcttctttca acgccgaatc aacatgttca
     5641 aagtttcctt gtcttacttc tttcaataat ttcaataatg tactgcgagc gtcgcctgaa
     5701 tatgcaacga tctcaaaacc aagcatttgt aattcctctt tattcatctt ttcttcctcc
     5761 attatcactt attaaataat cgttttggtt tcactgacca acttgtacca gtaagctgat
     5821 tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa acaagccata ccttttgtta
     5881 tacccatttg tccatgagaa aagatccata agcgaccaca aaaaataccc cttcacattt
     5941 accccggctg ttatcgcctt gcttaaggat tccaaataga ctctcaaata gtcgattctc
     6001 ggctgatcca taataatgcc atcttcaaac tgatctttat accccatccc gttctcagtg
     6061 atatagattt tgttgtaatg tgggtaatcg cttttaattc gcagcaacaa gtcatataga
     6121 ccttctggat aaatcagcca gtcccaatca gttcgaggaa ttccttcttt ataaatacgt
     6181 tctccgattc ccttcacttt ataaacagag gtcccttttt cacccgtacc gttatgatgg
     6241 attgcatttt ctccatcgta agctttgaca aaatggcatt ggtagtgatt gatccctaag
     6301 tagtcattac gcgtggatgc ttttttcaat tcaacgaaat cttcttcagg aaaatgatag
     6361 ctggcctgat ttgcttcaca tatctcatca agagcagtca aggtttcagt tgaataatag
     6421 cctaaatagg tagcatctaa taagaaacgg atcgacaatg catcgtctaa aaaggcagca
     6481 tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct ctaatgagtg aactacacca
     6541 atttcacccg gaagttcgtt ttctttgaaa tagttcacta cacgggcatg agcaaccatc
     6601 atattatgaa gacaggcgac gatctttgta aaatcatatt tgattcctgg tgggaaaaca
     6661 cctaacaaat attgattggt tgcaactgga tagatttcat taaaggtact ccacaccttg
     6721 acttctttga attcatgaaa acaaaaaatg gcataggaaa caaatgcttc tattgtttct
     6781 cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg tatcaaaatg atgcaacgtc
     6841 acaaacggcg ttacgtttct tttcttacat tcttcgaaca cacgatgata aaaagcgatt
     6901 ccttctggat ttggtttccc agctccatca ggaaaaatgc ggctccaagc aatggataat
     6961 cttagaccat ttacaccaaa acgttcacac aattcaatgt cctttgcata ttgatgataa
     7021 aaatcacttg ccggatcggg actgaatcgc ccttgttcct ctaaaaagtc atcccaagca
     7081 acagcgcctt tgccaccttc cttagtggct ccttcaacct gataggctgc tgtggctccg
     7141 ccaaaaataa aatcttctgg taatttaagc atgtacatct ctcctcaaat ctataatcag
     7201 ttttcgctct ttctaactga ttgcattgta aacgcatcca aaacatttgt caacaaaaac
     7261 aaacacaaat aatcataaaa accagaaaac agcaataaaa tctattgtaa caaacataaa
     7321 aaccacccta aatgtttact tttgttcgaa ttagattgac tgttgtccgt ttatatgata
     7381 gattatcctt aaattagagg tgaagacaat gttgaaaaaa gaaagattgc ttactatcgt
     7441 agaaatggtc aataaaaaag gaatccttac cgttaatgaa atcatcaatc aattagatgt
     7501 ttctgatatg accgttcgca gagatttaga tgaattagaa aaatcgggca agctgctgcg
     7561 tgtccatgga ggggctcaaa gtctttccta cactttagat caagaacttt ctcacactga
     7621 aaaagccact ctgcagattc aagaaaaaaa agaaattgct gccacagcag cctccttgat
     7681 tcacgaggga gaaacgattt tcttaggacc aggtacaaca atcgaactat tggcgacgca
     7741 tttactgaac aagaaaattc ggatcattac aaataattat ccagtttttg atattcttgc
     7801 tcaagcagat ggaccagaaa ttctactgat tggcggtgat ttccggaaaa ataccggcgc
     7861 atttgtcgga ccgatcacca acgacaattt aagaagacta aagtttacaa aagcatttat
     7921 cagttcaaat ggcgttcaca acgaagcgat ctctacttac agtaccgaag aaggagaagc
     7981 acagcagatc gctctaaata attcccgtaa gaaatatcta ctcgttgata ataaaaaatt
     8041 caatcgagat gatttttatg tgttttataa tttgcatgat ttcgatctgc tcattacgga
     8101 tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa tatgtcgatc tgcaaattgc
     8161 tgattcaacc gaaaatacca ctataggagc gtaactatga aagggattat atttgatttc
     8221 aacggaacaa tgtttttgga ctctcatctg catgaagctg cttggctgca tatgatacat
     8281 gatcatacaa aagatggttt atcagacgag gatattttaa aaaatattca tggtcggacg
     8341 aatacggaga ttttgaaaca tttcatttca gagcatttga ccgcctctga gatcgccgta
     8401 ttgtcagaag aaaaagaaag ctattatcgt atgctttgct tgcaaaatga aaacgagctt
     8461 cagttgacaa aagggcttga agcgactctt gaccagctga ctgaccaaca gattccatta
     8521 acgattgcca cggctactgt caaagagaat gttgcttttt attttgatat tttcgattta
     8581 gcccgctggt ttgactggga caagattgtc tatgacgacg gctctttccc tgggaaaccg
     8641 caaccggata tttttcttaa agcggcagac aaactgagct tagctccggc tgattgtctt
     8701 gttatcgaag atgcttactc aggattactt gctgccaaac gtgcggggat cggaacaatt
     8761 attgcaattg atccattcgg taaaaacaaa acagtatttg aacaggcaca gctaaataaa
     8821 gatggaacca tcaaagattt tactggtttt tggacagagc acctgtctgc cacatccgtt
     8881 aattcttaaa cgattcaact aactaccaaa gaaaagcaac tgaaatgcac acctcctttt
     8941 ataaaaaact gccagtgaaa cgacatgaaa gatacactgc tatatcctta gtgaaatata
     9001 gcagtgtatc tttgactttt tcattctttt aaccatatga cgcgttcatc tctatctata
     9061 attaacatta aaaatagtgt tttcacatta tttctcaaac caatcttaaa aatcatcagg
     9121 agtacttttt tgatatactt ttattaatgc tgggcgtaat ggttaaaaga tttccactag
     9181 ttcctctcca ccaagctgag ttgaaaaatg cttatctgta aacaacatag acaaagagga
     9241 aataaacgtt tccgtatctt tctctgtccc aaggatatta attaattttc ctactaggta
     9301 agtatcatta aacttaagat ctgccttcac atcaaaagtt ttgataaaag cttttatcat
     9361 gcgtacaatt ttgtctttat cgccataatt gatttcacca ctcatatcaa tatagtcact
     9421 aatccctgaa gaaataatgg caactgcttc ttctttcgcc ttatcagata attcatttaa
     9481 tgactgggat tgattgatga cttcttgagt tttatctgtt acgtaatctc tcattatcct
     9541 atcggcatac tccgcaaagt aatcttgaaa aaattcttct acagggtaac caacttcgtt
     9601 ttcccaacca ggagaaattt cattatcagt acttattaaa tctattaaaa accaacccgt
     9661 tctctgagtg ttgattctat aatattttta atttcctata taaatcaaaa atattataga
     9721 atcaataagc taattaagaa acaaacttta gaaactctag a
//
LOCUS       DAJSNV010000099         9649 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSNV010000099 DAJSNV010000000
VERSION     DAJSNV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.103"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            161..367
                     /locus_tag="ON516_002352"
     CDS             161..367
                     /locus_tag="ON516_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR3345815.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(569..1570)
                     /locus_tag="ON516_002353"
     CDS             complement(569..1570)
                     /locus_tag="ON516_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3345816.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1575..3487)
                     /locus_tag="ON516_002354"
                     /pseudo
     CDS             complement(1575..3487)
                     /locus_tag="ON516_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            complement(3655..4161)
                     /locus_tag="ON516_002355"
     CDS             complement(3655..4161)
                     /locus_tag="ON516_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3345817.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4321..4761
                     /locus_tag="ON516_002356"
     CDS             4321..4761
                     /locus_tag="ON516_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3345818.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4787..5944)
                     /locus_tag="ON516_002357"
     CDS             complement(4787..5944)
                     /locus_tag="ON516_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345819.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5947..6303)
                     /locus_tag="ON516_002358"
     CDS             complement(5947..6303)
                     /locus_tag="ON516_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345820.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6601..7575)
                     /gene="sgrA"
                     /locus_tag="ON516_002359"
     CDS             complement(6601..7575)
                     /gene="sgrA"
                     /locus_tag="ON516_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR3345821.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7771..8610)
                     /locus_tag="ON516_002360"
     CDS             complement(7771..8610)
                     /locus_tag="ON516_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3345822.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8795..>9649
                     /locus_tag="ON516_002361"
     CDS             8795..>9649
                     /locus_tag="ON516_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3345823.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTI"
BASE COUNT         2898 a         1948 c         1450 g         3353 t
ORIGIN      
        1 ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa
       61 gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta
      121 atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc
      181 tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta
      241 tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt
      301 gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca
      361 aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa
      421 atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc
      481 ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag
      541 attacaacct cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat
      601 atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat
      661 ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta
      721 cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg
      781 tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt
      841 ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac
      901 ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct
      961 gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg
     1021 ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg
     1081 accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga
     1141 taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt
     1201 gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta
     1261 catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg
     1321 tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct ttttttatat
     1381 cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac
     1441 ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca
     1501 aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact attcgcttga
     1561 accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca
     1621 gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac
     1681 attctttttt tgcatctgtc tttttttgta ctttcactgt catctgaatc ggttgcgtat
     1741 catcacttga ttgatcctct cgaggttcat tctttccctt attgtctgca ttctctgtcg
     1801 gatcttgctt ttttggtgga gctactggga tttttttgta atagaaaata atagttggta
     1861 tcgtctcatt aaaaatccct tcagtcggtc cttcaacatt tgctaattcg tatccttcga
     1921 atgtcttttg ctctgtcata taattttctc cataagctcc gtaaagctgg atttcgtcct
     1981 ggtctttgag tagactttta ttgttttcat ccacatactt tacgtttatc tgatcatgga
     2041 acactacttt caaaaagtcc gttacatgtt tcgtgtaggc agcatggtca acttcctgtg
     2101 cctttccatg ccctgctcct tctactaata aaagatcttt ataccctcct gataactcat
     2161 ataatttttg cgcgttagac acaggtacta catcatctgc tgttccatgg ataatcaatt
     2221 taggcaatga atctgacatt ccaatcgtag gtagagcagc ttttgtatcc aaatgcatct
     2281 cctgatcaaa atagtattgt ttcaaaaggc ccataaataa atcttcatgc tcttgatccc
     2341 caatgatgtc taactgattt tttatggcat ccggtagtgc gggggctact gtctggctgt
     2401 acgtaaaacg tagttgttca tcaattgatg caaatccgca attttcaata attcctttga
     2461 cattgacagg aatatctttc gctaatacag acatcatcgt agctgctccc atcgaaccac
     2521 cataaagtaa aatcttttga gatggccgct ctcgaacttc ttgatttatc cagtaaagga
     2581 cgtcatctga ctcatattgt ccataggtca cataatcgcc tccgctatta ccagtcgctc
     2641 tggaatcaac aaataataaa ttatacccgg ccttataaaa atcattgtat tcttctgtaa
     2701 cgatcccgtt attccaattc cctcgaaatc cgccatggat catgactgtt ttatctgaac
     2761 cttgatcgac ataatatgca tatacatcca tcattttgcc atcttgtttg tttagtacag
     2821 tttgatgaag ttgttttgta tttggataga agcctgtgtt ttctgtaggc gttgtcccat
     2881 catctgtacc tccaagcatc gacatcaaca cactgttttt tgcaaaagcg atatcgtaca
     2941 atagataagt agcaatcaga tgtttgataa tctctttatc tacaccataa tcattaacag
     3001 caagctcagt gactaataac tctattaatt ttgacttgat gtcttctgtt ggtccattaa
     3061 gtatttctgt catctgttta ccgaagtaat tcaaatattc tttaccattg aaattttcac
     3121 ctttaccgat atcatcattt tctgtcccat tctctttgtc acttacttta gcagaagagg
     3181 attcgtcgtt tgacattttt gtagctgttg cttcttctgt ttgttggtta tctgtggtag
     3241 cggtcgtttc ttgatctgtg ctagtaacag atgtctcaac cgttgtttca gtatttgttt
     3301 ctgtttgtga agtactcgat ttcgtttcat ttccagcatc agtagtttct gtggtttcta
     3361 cctcccgaga ttccgattcg gaagaaatgc tctctccata tactgtccat tgcgggacgc
     3421 ttgtacttac agcaaataag atgaccaacg ctccctttaa tacttgtttt gttctcaagc
     3481 tattcactcc tttttatgtt tctaggagaa ttatatgtaa acgcaaacat tttttataca
     3541 aaaaataaat ggtttatccc ataaaaatag aacaggatta ttttctcatt ttacgtaata
     3601 aagcagaatg gataaaccag tatccattct gcttttttta aacgaagaaa caacttattg
     3661 ctccaaaaat gtcagaaatc gtttatccca aattttctcg taaaaagcga ttgtttcctt
     3721 tgctttcata aaaggattat cataagttgt cgttgctcct ctacacatca taagctggta
     3781 tcctaatcca tgagcaaact tcacagtcgt atctatacaa tactctgctt gtgccccaca
     3841 taattcgatc gaatgacagt ccaattcagt taataaaaca ctcaaattag tcttataaaa
     3901 tgaattcgca tgggtttttc tcacaaaata actgtgctca ggaatagata attccgacaa
     3961 tagctcccat ggttttgttt gtggcactaa ttgagcatct tcgtgttgaa cgaaaataac
     4021 tggtttccca atcgtttcgt atgttttgat tcttttattc actaatgctg tcactgtttc
     4081 taattcgtgt atcggcgaag aatcaaaaca tacccctttt tgtaaatcga tcactactat
     4141 tgcgtcggct gtgtttgaca tacttgtgcc ttctttcttt tttcttgatt taatagtatt
     4201 tcttatctag ctagttccat tatacgtgac aacattttct tattttacac ctctaaatgg
     4261 cacttttcga ctatttcttc atgttattat ttcataaata cttagctatt ggaggaatgt
     4321 atgatgatta aattgtatac aggttctggc tgctcgtctt caagacgcgc aaaacgttgg
     4381 cttatcgtcc atgatatacc gtttatcgaa cagaacttaa ttcgtcaacc actaaccaaa
     4441 aatgaattta tccacttact aaaattgaca aacaacggcg tgaccgaaat gctctctact
     4501 cattctatta gttataaaaa tcttgttcac agaatagatg aactcagttt gagtgaacta
     4561 tatattgaga ttcgtacatc cccttcttta ttgaaaaaac cgattatcta cgaccaatct
     4621 gcaaaacttt tgactggatt tagcgaagat aaaatcagga tgttcctttc taaaactaaa
     4681 agaaagcaag agctcaacga actcttgctt tctggtcatc ttgtcgattt taaagaaaat
     4741 aatctttttg caatgtctta attttggcat aaacaatctg cttttcttaa ttaacgaaat
     4801 ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt tcagctttca
     4861 gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt gtattcgttt
     4921 gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt aactgatcag
     4981 cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc tttgcccata
     5041 attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt tcgtgtttta
     5101 taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc ttttcataat
     5161 aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc atgattggat
     5221 acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca aaagcatcca
     5281 ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa ttgacatata
     5341 gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa ttttctacaa
     5401 tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc aacaaatctt
     5461 cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc tctgttggag
     5521 tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg taatgcgtaa
     5581 actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg acatacgtaa
     5641 tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc catttctttc
     5701 tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt tgacgtttat
     5761 tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa tacatcaagt
     5821 gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt ttcaagctgc
     5881 aagataaatg atagtttttc aaaatagtat taagttctct taattttctt tgaatcgtac
     5941 gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac ctttgtaaag
     6001 tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa ctttactcag
     6061 taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa aagataattt
     6121 atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat tcatcttagc
     6181 cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct cgtctctttc
     6241 ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa attcttcaaa
     6301 caaagtacat actccttttt atctattatt tagacttatt ccgacagcta catgaataac
     6361 gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg tgcattgtca
     6421 attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct caaatatgtt
     6481 agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag cactctcgaa
     6541 tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg cttttattct
     6601 ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca aaatgagtaa
     6661 cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg gtaaatcgct
     6721 ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag aaacaaaatc
     6781 aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt ccttagttgg
     6841 tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt cgctgctcgt
     6901 gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac tcgtactctc
     6961 cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
     7021 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
     7081 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
     7141 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
     7201 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
     7261 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
     7321 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
     7381 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
     7441 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
     7501 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
     7561 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
     7621 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
     7681 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
     7741 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
     7801 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
     7861 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
     7921 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
     7981 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
     8041 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
     8101 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
     8161 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
     8221 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
     8281 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
     8341 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
     8401 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
     8461 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
     8521 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
     8581 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
     8641 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
     8701 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
     8761 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
     8821 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
     8881 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
     8941 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
     9001 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
     9061 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
     9121 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
     9181 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
     9241 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
     9301 agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
     9361 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
     9421 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
     9481 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
     9541 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
     9601 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatt
//
LOCUS       DAJSNV010000100         9631 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSNV010000100 DAJSNV010000000
VERSION     DAJSNV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.104"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..1183
                     /locus_tag="ON516_002362"
     CDS             185..1183
                     /locus_tag="ON516_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3345824.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ON516_002363"
     CDS             1295..1804
                     /locus_tag="ON516_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3345825.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ON516_002364"
     CDS             complement(1834..2493)
                     /locus_tag="ON516_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3345826.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ON516_002365"
     CDS             complement(2495..3259)
                     /locus_tag="ON516_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3345827.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ON516_002366"
     CDS             complement(3267..3944)
                     /locus_tag="ON516_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3345828.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ON516_002367"
     CDS             complement(3981..5366)
                     /locus_tag="ON516_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3345829.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ON516_002368"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON516_002368"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON516_002369"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON516_002369"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3345830.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ON516_002370"
     CDS             6305..7162
                     /locus_tag="ON516_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345831.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ON516_002371"
     CDS             complement(7243..7578)
                     /locus_tag="ON516_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345832.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ON516_002372"
     CDS             complement(7645..9015)
                     /locus_tag="ON516_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3345833.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ON516_002373"
     CDS             complement(9093..9629)
                     /locus_tag="ON516_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3345834.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2822 a         1986 c         1618 g         3205 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt c
//
LOCUS       DAJSNV010000101         9423 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSNV010000101 DAJSNV010000000
VERSION     DAJSNV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.105"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..459)
                     /locus_tag="ON516_002374"
     CDS             complement(37..459)
                     /locus_tag="ON516_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3345835.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(449..2623)
                     /locus_tag="ON516_002375"
     CDS             complement(449..2623)
                     /locus_tag="ON516_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR3345836.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2639..4981)
                     /locus_tag="ON516_002376"
     CDS             complement(2639..4981)
                     /locus_tag="ON516_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345837.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5003..7546)
                     /locus_tag="ON516_002377"
     CDS             complement(5003..7546)
                     /locus_tag="ON516_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3345838.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7556..7954)
                     /locus_tag="ON516_002378"
     CDS             complement(7556..7954)
                     /locus_tag="ON516_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3345839.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8035..8319)
                     /locus_tag="ON516_002379"
     CDS             complement(8035..8319)
                     /locus_tag="ON516_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345840.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8343..9341)
                     /locus_tag="ON516_002380"
     CDS             complement(8343..9341)
                     /locus_tag="ON516_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3345841.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2681 a         2012 c         1470 g         3260 t
ORIGIN      
        1 ttttgttttt ttcaacgtat tctctttcaa ttttcactaa aaattaggaa gaaattgatt
       61 caatgtcttt tcaaaaaaga atgtttttct ctcaattttt ttgacgtttc tatcatgaat
      121 cacgagaaaa cttgggacac ttttaacatg gtaacgttgt aaaaaagttt gaagttcttc
      181 ttgtcttggt tcttttcttg tttcaattcg atatacattc ctagagaatt ttttgtcgta
      241 tctttccaag aaagattcaa cttcttggca ttttttacaa tcatttcttg ctagatacac
      301 aaaaaatgat tgattcgttt tttgaagttc tagtacttct tctaaattac tattgattag
      361 ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata agaggaaact
      421 aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc aaatttggtt
      481 tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga tacaccacaa
      541 attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg tctacgtctc
      601 gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa cctgcttctt
      661 gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct gcccaataat
      721 caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc ccaatcaact
      781 gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg gcatacgccg
      841 catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca tttagataag
      901 aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct cctgcttgtt
      961 tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc aatgtccgaa
     1021 taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct tctttaacaa
     1081 cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca ccaagctcac
     1141 tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac aaataaaacg
     1201 ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc tcgatttctg
     1261 tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca acggttttac
     1321 gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa tcacgttctg
     1381 ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc ttttctacga
     1441 ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca aacaaatgta
     1501 agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca attttatggc
     1561 tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg gctgctgtcg
     1621 aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg gcaatatcat
     1681 tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact tcaatcgggc
     1741 gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct cctttcccat
     1801 cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat tggatcaccg
     1861 tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat tcactaaagt
     1921 tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg gctaatgaag
     1981 ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg tacgcttgct
     2041 gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt tccttgtatt
     2101 ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt cttttttcaa
     2161 taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa aggagggcaa
     2221 aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga aataataagt
     2281 aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt aatagcgtaa
     2341 aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc tcatactcaa
     2401 agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc gctaaaggaa
     2461 tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg ataaaataaa
     2521 gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga tcgacagaaa
     2581 acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg atttcctcct
     2641 atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt tttcgtttca
     2701 tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt ggtgattttg
     2761 tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt tgtgctgtta
     2821 ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca gatttcggtg
     2881 tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt accgcatgtt
     2941 tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga gtaagtgcct
     3001 ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat tctgttctag
     3061 gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat gacaccacca
     3121 ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt ctttctctta
     3181 gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt gcctgtttct
     3241 ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc gcttgatcat
     3301 gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta tgacgaggtt
     3361 gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt tgtggcatac
     3421 gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt gcgttttctt
     3481 tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt tgtttggctt
     3541 cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac gctcctcgaa
     3601 cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa attcccaacg
     3661 gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct tgtgtactac
     3721 ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa cctgtcattc
     3781 cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca ttcattgtga
     3841 caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat agttgcatag
     3901 ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga atcagattca
     3961 cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat tgtatgttct
     4021 tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc tcccctattt
     4081 ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt ccagcatatt
     4141 tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac aacagatatg
     4201 gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc gtttccactt
     4261 ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct tcttcaggtg
     4321 ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa gaaaaaagaa
     4381 catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca gtaaaaataa
     4441 ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca gcatatttga
     4501 ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt aacatattgt
     4561 ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata atatctaatt
     4621 caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg ccactccaaa
     4681 caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg ttcattgact
     4741 catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac aattgatcca
     4801 catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct tcagaacgat
     4861 cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta gccccttgta
     4921 gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat ttttttcgca
     4981 aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt gcttgaattt
     5041 tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac aacacatcaa
     5101 tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct gtttgtaatt
     5161 gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac aatagtttct
     5221 ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac tcttttttat
     5281 cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta ttattcgcac
     5341 gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct tcataccctt
     5401 ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag atatacgtaa
     5461 acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt aatcgacccg
     5521 aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg ttgatttgtg
     5581 aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct aaaccaacgc
     5641 tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct tcgataactt
     5701 gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct tggatcaccg
     5761 ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt cctaacacat
     5821 ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca agcatccctc
     5881 gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata gccacaaact
     5941 ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt tcatttttcg
     6001 gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata tattttacaa
     6061 ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg gtatgttccg
     6121 cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta ctagatagcg
     6181 ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct aaaggaaatc
     6241 ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca aaacctgtcg
     6301 tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat tgattaaaca
     6361 acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca atcgcaaatt
     6421 caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca accagcgtga
     6481 cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca tccattaacc
     6541 gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg gtctcatatt
     6601 ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca tcagattttg
     6661 aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta tcctgaagat
     6721 tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt aaaaacgtcg
     6781 aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc gaaatgacgc
     6841 cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct gatcgatgga
     6901 aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc ggcttataat
     6961 tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac acactactta
     7021 taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc ttaagcaatt
     7081 gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca aacagatacg
     7141 ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt tcaaagtaca
     7201 ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct tcaatgtgcg
     7261 tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca tcatgtccaa
     7321 gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt aaaggaaata
     7381 cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa acaaaatttc
     7441 ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc tttttgaact
     7501 gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac tcgactcact
     7561 ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca tacatttcat
     7621 gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa tactcgaaaa
     7681 agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca atcccaaaaa
     7741 tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga ttcacatacg
     7801 caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact aattgttcca
     7861 tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa attttgtgag
     7921 ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct tttacaaaaa
     7981 aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt tttcttatga
     8041 taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac tatccttaat
     8101 aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca ataagacgag
     8161 cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc cttgggtcgc
     8221 ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga cttgtccaga
     8281 tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc tctaagttca
     8341 ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac ttccattgaa
     8401 aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc ttgcaaaaag
     8461 tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa ttcgccgcct
     8521 tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac atataactca
     8581 aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac cgcttgccca
     8641 gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact ctcagaaaga
     8701 ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact gactggaaaa
     8761 gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata aaataggcga
     8821 tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc tttcgctact
     8881 cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg tgctaaataa
     8941 tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa taacctttca
     9001 ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg gttgacttca
     9061 gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa tgtatccact
     9121 cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa aaaagcgcct
     9181 gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga cggttctgtt
     9241 ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg tttcggtggc
     9301 ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc cataccgttc
     9361 taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca cacttgcccc
     9421 ctc
//
LOCUS       DAJSNV010000102         9252 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSNV010000102 DAJSNV010000000
VERSION     DAJSNV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.106"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..651)
                     /locus_tag="ON516_002381"
     CDS             complement(<1..651)
                     /locus_tag="ON516_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3345842.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPL"
     gene            785..1072
                     /locus_tag="ON516_002382"
     CDS             785..1072
                     /locus_tag="ON516_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345843.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1488..3026
                     /locus_tag="ON516_002383"
     CDS             1488..3026
                     /locus_tag="ON516_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3345844.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3023..3988
                     /locus_tag="ON516_002384"
     CDS             3023..3988
                     /locus_tag="ON516_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345845.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3975..5297
                     /locus_tag="ON516_002385"
     CDS             3975..5297
                     /locus_tag="ON516_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3345846.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5360..5464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5538..6281
                     /locus_tag="ON516_002386"
     CDS             5538..6281
                     /locus_tag="ON516_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3345847.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6296..7288
                     /locus_tag="ON516_002387"
     CDS             6296..7288
                     /locus_tag="ON516_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3345848.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7300..8037
                     /locus_tag="ON516_002388"
     CDS             7300..8037
                     /locus_tag="ON516_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345849.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8027..8749
                     /locus_tag="ON516_002389"
     CDS             8027..8749
                     /locus_tag="ON516_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3345850.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8833..>9252)
                     /locus_tag="ON516_002390"
     CDS             complement(8833..>9252)
                     /locus_tag="ON516_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3345851.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3025 a         1598 c         1959 g         2670 t
ORIGIN      
        1 aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac
       61 agatccaatt tccagatact tcactttatt caatgtataa gatagaatag aacagctaag
      121 taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa
      181 agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata
      241 aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc
      301 agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg
      361 aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag
      421 aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag
      481 tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac
      541 cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc
      601 tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca tttctagcct
      661 cttttctttt gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa
      721 gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg
      781 aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta
      841 tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa
      901 taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga
      961 catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga
     1021 actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat
     1081 aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt
     1141 gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat
     1201 ttctacttta ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat
     1261 agctgattcc aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag
     1321 aaccaaaaag ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg
     1381 tatttttttc ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa
     1441 aaggaaagaa atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta
     1501 tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca
     1561 tttctctcat ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag
     1621 ccaatcataa aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc
     1681 cttcacttgc cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat
     1741 tgattgcact tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag
     1801 ctgaaattga cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac
     1861 gcttgctgaa agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg
     1921 ccttagtttt aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag
     1981 ggacatttat tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga
     2041 tcagttctgc cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga
     2101 aacctcgata ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt
     2161 tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg
     2221 aacctgaaat cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag
     2281 ttgaattaaa accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc
     2341 aaatcgatat ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg
     2401 aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat
     2461 tgaataagca attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct
     2521 tggcagtcaa gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag
     2581 ctatctctga tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat
     2641 ataaaggaat ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa
     2701 gtttgcgtta ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag
     2761 atagccagct caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag
     2821 cttatcaagg cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta
     2881 aagcactgaa taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc
     2941 aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag
     3001 gattagtaaa ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa
     3061 gtatgatgga aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt
     3121 tgttttggtt ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt
     3181 atctaaacca tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct
     3241 gcagaaaatg cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat
     3301 gacagtcaag caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag
     3361 agcagaccgt gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga
     3421 tcgtatgcct agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat
     3481 tgctgcaagc ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc
     3541 aagaaactca ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt
     3601 tttcgttacg cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa
     3661 ggatggagaa attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga
     3721 ctacgttcgg catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt
     3781 agtcgtcaat ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt
     3841 tgatacgcct atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt
     3901 agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg
     3961 agaggaggaa ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct
     4021 taagtgctgc ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc
     4081 tatggggcgg gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag
     4141 tagaggcacg taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg
     4201 ccaactggga agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc
     4261 gaaaaaaaca attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg
     4321 atgattcatc tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa
     4381 ccgggcaaag acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag
     4441 actttttatc attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg
     4501 cttttgagct agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata
     4561 atcaacggcc attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg
     4621 aagcatctgg cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta
     4681 ctcgctatcg tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga
     4741 ctgggagaca gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata
     4801 aacacggaat tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg
     4861 gagatattgt ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt
     4921 atgtagctaa gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta
     4981 cgatcgtcta cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt
     5041 cgtcagatca agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg
     5101 aaccagttgc taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag
     5161 tgattagcga gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat
     5221 tgactaaaaa agagttggat agctatatca tggggtatcc gacattggca agtgatctct
     5281 cctatttatt gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg
     5341 ctacacttta aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt
     5401 tgtgaactta gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga
     5461 cgcttgaagc gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga
     5521 agaaaggagg taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt
     5581 taattatttg gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac
     5641 ctgctgctgt attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca
     5701 cgacattaaa tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg
     5761 ccttggcaat ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt
     5821 cacaaatgtt gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta
     5881 tgcttccaaa agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt
     5941 cggatacatt gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag
     6001 atacagggaa gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg
     6061 gtttaaaagt ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg
     6121 ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag
     6181 tttttgctgc gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat
     6241 tattgaaaaa agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa
     6301 aaaattatgg cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga
     6361 aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt
     6421 accgaatact aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc
     6481 tggtttaaat gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg
     6541 tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga
     6601 tggacagaat ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt
     6661 cgccacccta tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc
     6721 aggttggcaa agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg
     6781 ggacttttca aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa
     6841 atcctcagat atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg
     6901 tattgaacta aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc
     6961 ggtaattatt acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat
     7021 ggatgccacg aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact
     7081 gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt
     7141 gtcaaaaaat tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg
     7201 caattataca gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga
     7261 actattcact aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac
     7321 ttgaatgtag tcattgaaaa cctgaaagta ttagaagaca tccatttcac tatccctaaa
     7381 gataccttta catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact
     7441 ttaacaggag tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact
     7501 ggtctccata cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc
     7561 gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa
     7621 gtaagggatt atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa
     7681 ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct
     7741 tacttgcttc ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa
     7801 gactggatgt tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat
     7861 atcgaagaag caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat
     7921 gtgattgctg agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa
     7981 tcagagttga agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc
     8041 tcattgtcat ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt
     8101 gacgatcatc aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag
     8161 taagactcaa gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga
     8221 acaagttgag ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat
     8281 ttggacgaat gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc
     8341 agccataaat gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa
     8401 ttatatccaa gcttacccta atcactattt gatccattgg tattcttctt tggactatca
     8461 acaagactat attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc
     8521 agcagtctgg caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg
     8581 actacatgca gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact
     8641 gcttgaagaa cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa
     8701 aaaaaatact tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt
     8761 gacacaacta gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt
     8821 aataaatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga
     8881 accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc
     8941 accatttaag taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt
     9001 gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg
     9061 agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg
     9121 tttttccgtt tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg
     9181 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
     9241 ttgtcctgag ta
//
LOCUS       DAJSNV010000103         9093 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSNV010000103 DAJSNV010000000
VERSION     DAJSNV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.107"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(56..880)
                     /locus_tag="ON516_002391"
     CDS             complement(56..880)
                     /locus_tag="ON516_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3345852.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(853..1533)
                     /locus_tag="ON516_002392"
     CDS             complement(853..1533)
                     /locus_tag="ON516_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3345853.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1556..2494)
                     /locus_tag="ON516_002393"
     CDS             complement(1556..2494)
                     /locus_tag="ON516_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3345854.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2739..3602)
                     /locus_tag="ON516_002394"
     CDS             complement(2739..3602)
                     /locus_tag="ON516_002394"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3345855.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="ON516_002395"
     CDS             complement(3744..6317)
                     /gene="mprF"
                     /locus_tag="ON516_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3345856.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6458..7264
                     /gene="proC"
                     /locus_tag="ON516_002396"
     CDS             6458..7264
                     /gene="proC"
                     /locus_tag="ON516_002396"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3345857.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7343..8899)
                     /gene="rny"
                     /locus_tag="ON516_002397"
     CDS             complement(7343..8899)
                     /gene="rny"
                     /locus_tag="ON516_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3345858.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2680 a         2014 c         1565 g         2834 t
ORIGIN      
        1 aaaaagaagc ctataaaaaa tttttcgttt ttataaggct tcttttcttt ataatttagt
       61 tattttttct tctgaaggca accaatattc gtttgatgat atttacaatc aggaacaaag
      121 caataaagac taatgtgatt gttgctcctg gaggtgtatc tagataaaac gagctagtta
      181 gtcctgacag catccctaga aatcctacac aaacactgat agcaatcacg acattgaagc
      241 ttttgcctaa tcgcataccg attgctgcgg gaagaaccat gattgcggat atcaaaagtg
      301 caccggcaat cgggatcatc acagcaatag ccacaccagt aatcacattg aacatcatcg
      361 acatcaaatg aacgggtagc ccatccacgt gggcggtatc ttcatcaaaa gtcagaacat
      421 acatcggccg tttgaataaa aagaacaata taccgatggc aacgaataag attcctaaaa
      481 agatgacttg ctcccatgta atggtcacga tcgaaccaaa cagataggat tggatacttg
      541 ttgctgaatt ccctccgctc aaattcatca atactaacgc tagcgccaaa ccaccagcca
      601 ttaaaatggc aatggagatt tctgaatagg ttcggtagat cgttcgcaga tactccaata
      661 tcacagctgc taatacaacg actagcatgg tcgtaagtgt aggattaagg ttcaaaaaga
      721 atcctaacgc tacccctgct aaagagacat gagaaagcgt atcagccatc agcgattgtc
      781 ttctgatcac taaaaacaca cctagcatcg gggcgattcc ggcaatgaag atagcggcaa
      841 gaaacgctct tctcatgaat tcatatgaaa gcaacgccat tgagaatcct cctttctcac
      901 cagtcggatc tgtctgtccg catactgttt gatatcttca tgatcatggg tgatcattag
      961 aactgatttc ccatggacat gcgcactatg tcggagaaga cgataaaatt catttctgga
     1021 ttcttcatcc atacctgttg ttggttcatc aagaacaaat aagtcagggt cagtagcaaa
     1081 aatacgcgct aaactgattc gctgcttttg tccgccagac aattcaccta ttcgccgatt
     1141 tcgcatatcc cacatcccta cagcttctaa agcacgctgt acatgttgat gatctttttc
     1201 agagagacgc ttgaaccagc ggcctcttgg atatcttcct gaacggacta attcgatcac
     1261 cgtactagga aaacctgcat tgaaagatgc aacttgttga ggaatataac cgatacttaa
     1321 tttttttcct tcttgatttt tctcagcaat ctttatcatt ccttttgaag gtttcagtaa
     1381 tcctaacgat gctttcacca aggttgactt ggctgctcca ttttctccag ttaaaatgac
     1441 aaattcaccc gcatctacat gataagagac atcttctagt acaggttcat catcatagta
     1501 aaaggtcaaa tcagatattt cgatataacg caccttacaa cctccattct aaaacttaat
     1561 tgatactttc ttttaaagct ttcagatttt tttccatgac agaaatataa ttttcgcctg
     1621 ctttgatttg ttcattagtc aagctttcta aaggattcaa cacttctagc ttcactcctg
     1681 tttcattcga caatgtctca gctaccttcg aagaggcatt ttcttcaaaa taaatcactt
     1741 tgatgttgtt tttcttcaca aattctttta gctcagctaa gcgacttggt gtgggttcct
     1801 gatctggtga aacgcccgaa atcgctactt gttttaagtc gtattgattc gctaaataag
     1861 caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc atcttttagc tcatcggagt
     1921 atttttgatc cagtgcttca agctttttga tataagcatc ggcattcttc gtaaaagttt
     1981 cctttttatc tggatatttt tcgcctaatt cttcagcaat cgttctgact tcttttatag
     2041 ctaataccgg atcgagccaa acatgtggat cgtattcatg tgaatgacca tgcccttctt
     2101 ctgattcttc atgttcgtct gcttcttgcg attctaaacg gtcgattcct ttagctgctt
     2161 cgatcatcaa ggtctgtttg ctatccactg aatcctttaa cgaatccaca aaaaattcca
     2221 tgtctgaact attatacacg aaaacatctg catcggaaat ttctgctctt tcttttgcac
     2281 ttggttcgaa atcatgcggc tccgttccag cagggatcaa aagtttcacg tttccttcat
     2341 cccctacgat ctcttttgta aaatcataca tcggataaaa agtcgtaacg atcgtcaaat
     2401 cctctttttt gtctgcttcc ttgttcgtat ttccacatgc tgcaaacagt aaagcaccta
     2461 tcattaccgt agctgtcaaa agatattttt tcatttatat gctcctttca atgaacaaat
     2521 cggaatttta cgatttatta acgtgaacta atgtatcaaa ataaaaaaac acagtcaata
     2581 gaaatccatc aatttctgat ttaagcatgt atattcttga ttaaatcgta aaatggatga
     2641 gttgctgctc atatctagcc atttcactac aataaaaaac tgaagaatac cattttagga
     2701 ttcctcagtt tttcatcacc tattttactt cttatatttt attgcagggt tcgtactaaa
     2761 taaacttcct cacaaaaacc ttttaaccca ttatatactc tctgcgctct tgaataatgt
     2821 tgacacaagg caaaaacggt tggtccgctt ccactcatca atgcagcatc tgctccgtac
     2881 ttgatcatac gctctttgat ttgatggatg actggatgcc gctttcccgt tatatcttct
     2941 aaactgtttc ccattttttt gatcatctgc tgataatctt gctctcggat ggcttcagca
     3001 agtccgtgga tgtcttgatg ttggatggct tgcgtatcta cttctctaaa cactgtacgt
     3061 gtagagacac tcattcttgg tttgaccaac acaacccagc actgtggcat agaaggtaat
     3121 acttggattt cttccccttt gccaccgaca aatgccgtac ttccgtagac acagtaagga
     3181 acatctgtac cgacttccaa accgatttct gccagttctt ctaacgaaca gcccagattc
     3241 cataattggt tgatccctct tagagcagct gcacagtctg tacttcctcc gcctaatcca
     3301 gctgctactg gaatattctt cttaacaaag atcttgacac cggttttgat attgtattta
     3361 ttttttagca ataaagcagc ttgataaaca ttgttccgtt catctaccgg taaaaatgct
     3421 ttatttgttt ctacaatgat tttgtcttct gttatttctt caaaaaatag atgatctgcc
     3481 aagtctacgc tcgacatcac catttccaac tcatgatacc catcttgccg tctatataat
     3541 acatccagtc ctaaatttat cttggctggc gctttttcaa tcaattccat cctctcaccc
     3601 attcgaaaag tctaagtcgc aagtattcta tcatagggaa aaataaaagt atagcttatg
     3661 tctgcctagt ggttgatttt gtcatcgttt caacagcttt cacagaattt tctcttcata
     3721 atccaaacaa gttctagtga ttcttataat tctgtgagca cttcttctgc caaactcgga
     3781 acatttcctt tttcttttcc tatcagactg agtagctgca tggtagcaaa tccgaaatta
     3841 cttcctttcc tataagcaaa ataacgtcct tcccaatgat cgacatattt taatttcttt
     3901 tcataagcag actggaagga atagaaaaaa ttaccataat tatatacgat atgcatgact
     3961 ttttcttgaa agaaagaaaa atctgtttct ccaacattag ctaatggagc taagcctaga
     4021 ttggcttccg agtagcccat tttctcgtat acaataaaca gattggctaa cagatagtca
     4081 gctaattcct cagattcttt aggtaagacc cgcaacaaat catatcccgc ctgcttcttc
     4141 gtcatcggct gagcggtgat gaagccaacg actttttgat ctttacggac aataccgaca
     4201 ccgctagata atacatatga tttatcaaat cgtcccacag agaagtattt ttctcgtgca
     4261 ccttgaagcc atttttgtga aacttcattt acagcttgat gtaattcttc cggcagttga
     4321 tcgtaccaag taaaatggta tccttctttc atcaaacgtt gatattcaag ttgctgactc
     4381 agccgagaca agtcttctgt tgctagatca acaatccctg cttcacctac tttcgtgaag
     4441 tgaaaaccca attcatgaag catcactacg tagctttctg atatcttata aaacgcaagc
     4501 tgatagccta aacaatctgc ttgtttcata aactgattcg ttgctgcccg catcatttta
     4561 ggattgccaa ctggatctcc taaaacaaag catttgtttc ctttgatctg ataaccaaaa
     4621 acgacttgat cttggtcgtc ttcttggtaa taaaaataac tgtaacctga caagcgaagc
     4681 tgatggcttg tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg gtctttttcg
     4741 tactcactgc caagcttcgt agtatcttca gctaaatatt gataaagcgc cactaaagct
     4801 agcaatgaga tgcccaaacc aataagccct gagaaccaca catcatctga aggaaacaaa
     4861 ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata gcctgcaatt
     4921 gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa agattatagc tccccaagaa
     4981 taaacgaatt ttgttcgata aaattctttt ctggataacc acacagctaa cagaatcaag
     5041 acatatacca atatcaactg ccaagataac gtacgtgtga ttgtatttaa aataccaaaa
     5101 gatagtaaag caatcgtagg cagaaatgcc tttttgactt ttactgaaag tgctctagca
     5161 aggccgagta ataaaaagcc aaccaataga ttcactgtct gatctaaaaa attgaatgag
     5221 aacggcaata atacttgaaa tagccgactg attgcagaca gattagtgat tgttgataga
     5281 agtaccatca tgatccccgc aaaatagaca gcacttacga gcacgatatg cgcaaccttt
     5341 tggaaaaaca gtcgcggaat gttatctaaa aatcgattga ttttgactcc tgtatgtgtg
     5401 atgaacaata gaagtcccgt cataaaaggt agaacataat aaaacaaccg ataatataat
     5461 agccaaacta ctgctacatc ttgccgaaca gacaattgac tcattcctaa gatcatcaaa
     5521 acgtcaaaag tccccattcc acctgggacc atcgtcagca ttcctatcaa tgtagcaatc
     5581 atgaacatag gatagatcga aagcatcgaa atatctattc tcatcaactt gccgatgatc
     5641 aaaaataccg caagggctcc cgtccattgt ccaaaggatg ccagtagcag accaagcaag
     5701 ccttttggat aaaactcgaa gaacaatgtc tttctcttta gataagcaaa aaagaaaatg
     5761 actggtgcta aaaaactacc gccgaaaagc cagatccaat agcttcgaaa aatactttct
     5821 gactgaataa aaaagacatc aataaatgta agaaacgacc atacagacaa accagtcagc
     5881 ataaatagtg cgacttttga tacagtggct aaaaccattt ttctatttgt ctgctttcca
     5941 taaaaactag cccgcagact tgctcctacc acaccaccaa aaccggcgag attgttgatc
     6001 gtgtttgtta cccacgcagc aagaagaaac tcttttcttt ccatttttgg ttttccttgt
     6061 ttttctaaga ctctgatcgt cacccaatca tacaacaaca tcggagtgac acccagcagt
     6121 ccagccagaa tcatgagcag gatcgtcatg cggctttgtt gtcccatcct ttgccaaata
     6181 tcttgccaac tcatgccatt agcaatattt gcgacttgat ttgccacaaa gactaacacg
     6241 gagccgaaaa aaatcagttt taaaatcaga ctatgttcct ttatccattg cagcatcaag
     6301 tatcgtttat ttttcacatt ttcccattcc taacttgtat acttttgtgt cataccccaa
     6361 tgttatctta tttattttaa tttttccact ttagacctgt tctttgcttg cgatgataga
     6421 aaaagaaaga tatcctaatg ctaaaggaga ggataacatg aaagtaggat ttatcggcac
     6481 agggaatatg gcacaagcta ttattgatgg tttattaaaa aaaggaacag tctcgaaaga
     6541 agaggtgttc gtttccagtg gacattttga aaatgcacaa aaatttgctg ctgaaactgg
     6601 cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg ttcttttagc
     6661 cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta agagaatata gccagaaagt
     6721 cttattcatt tctattgctt caggaaaatc attaatagat ttagaaacag cgcttgaaga
     6781 tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag gagcaggcgt
     6841 ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag cttcagcaag ctacgatgat
     6901 ttttgaagca atcggcagcg tctatccttt agcagaatca gatttcagta cattcattgg
     6961 ccttgctggc agctcacccg cattcgttta catgctgatc gatgcgatgg gacggactgg
     7021 tgtactgaat ggtttgtcca aacagacagc cactgagatt gctgcaaaag caattatggg
     7081 ttcttgtcaa aaattccttt caagtgataa aaatccatgg gaacttgtag accaagtttc
     7141 ttctcctggc ggtacaactg ttgcaggtgt agttgcctta gaagaagcag gattcatccc
     7201 agcaatcatc aaaggaatca atacgacgat cgagaaagat cttgagatgt tgaataaaaa
     7261 ataatgatag caatgttttt tcgtgcctta caaaaaaaga agagctgtgg cttttgccgc
     7321 ggctcttctt ttgtattttt tattatttag cataatcagt tgcacgagtt tcccgaataa
     7381 cgattacttt gatgtgtcca ggataatcca gttcttcttc gattcgttta cgaatatcac
     7441 gtactaaacg aacagcgtct aaatccgaga tttcttctgg cttgaccatt acccgaactt
     7501 cgcgtccagc ttgaactgcg taacttgatt ctacaccggc gaagctgttc gaaatatttt
     7561 ccagacttct taaacgacgg atgtagtttt ctaatgattc acttcgcgca ccaggtcttg
     7621 ctgctgagag cgcatctgct gcagctacaa ggacagagat aacagaagta gcttccacat
     7681 ctccatgatg agaagcgatc gcgttgatta ctactgggtt ttctttgtac ttggcagcta
     7741 attccgcacc gatctcaacg tgagatcctt caatctcatg gtcaagtgct ttcccaatat
     7801 catggagcaa cccggcacgt tttgctaatt ggatatcttc gccaagctct gctgctaaga
     7861 taccagctaa ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca taactagttc
     7921 tgaatcgcag acgtccgatg atcttgatta gatctggatg aagtgtatga gcaccgactt
     7981 caaacgctgc ctcttccccg tattcgcgaa tacgttcatc catttcttta cgagatttct
     8041 cgaccatttc ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt ttttccaagg
     8101 tcatccgtgc gatttctctt cggattggat cgaatccgga aagaacaact gcttccggtg
     8161 tatcatcgat gattaaatcg ataccagtca gcgtctcaag cgttcgaata ttacgacctt
     8221 ctcgtccaat gatacgacct ttcatttcat cattcggaag ggtaacgact gaaacagtcg
     8281 tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa tgataataag tttttcgctt
     8341 tgcgatctgc ttcttctttg gcacgctgtt cagattcttt taccattaga gttaactcat
     8401 gattcagctc ttcttcagtc gatttcatga tgacatctcg tgcgtcctct tttgataaac
     8461 cagcaatttt ttctagttct ttttgttgat cttcaatcat tttttcaacg tctttttctc
     8521 gctcttcaat taattgctgt tttgaactaa gtgcttcttc tttactttca agtgagttct
     8581 cgcgtttttc taacgagtca tctttacgat tcagcgtttg ttcgcgttga atcaaacgat
     8641 tctcttgaga ttttaattct aacttacttt ctctcagctc actttcaact tcagaacggt
     8701 atttctgatt ttcttcctta gcttctaaca atgcttcttt ttttagagtt tctgcttctt
     8761 tttttgcact atcgatgatt ccagcagctg atgattgtgc accagctatt tctttctcgt
     8821 gacgtgactt tgcgaccata aaccctaaac caagaccgac aattaaaccg atgatagcga
     8881 ggagaatgtt gaataccata aaatccacct ccatactatc tttttcattt ttatcatcta
     8941 tacagcttct atgataaatt attctaaact cgtcaatatg catcttcgca tatatgtgta
     9001 caaaaaacat gcacaacttt attctaatgt ttgtaccccg tagtgtcaac tttaaataaa
     9061 caaagaaacg gctttctact tattcattaa acg
//
LOCUS       DAJSNV010000104         9057 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSNV010000104 DAJSNV010000000
VERSION     DAJSNV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.108"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..260
                     /locus_tag="ON516_002398"
     CDS             51..260
                     /locus_tag="ON516_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345859.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ON516_002399"
                     /pseudo
     CDS             467..667
                     /locus_tag="ON516_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ON516_002400"
     CDS             complement(1379..1984)
                     /locus_tag="ON516_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3345860.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ON516_002401"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ON516_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="ON516_002402"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="ON516_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="ON516_002403"
     CDS             3019..3990
                     /locus_tag="ON516_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3345861.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ON516_002404"
     CDS             4130..5443
                     /locus_tag="ON516_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3345862.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ON516_002405"
     CDS             5486..6352
                     /locus_tag="ON516_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3345863.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ON516_002406"
     CDS             6349..7197
                     /locus_tag="ON516_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3345864.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ON516_002407"
     CDS             7211..8683
                     /locus_tag="ON516_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3345865.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>9057
                     /locus_tag="ON516_002408"
     CDS             8700..>9057
                     /locus_tag="ON516_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3345866.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGS"
BASE COUNT         2860 a         1537 c         1860 g         2800 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtg
//
LOCUS       DAJSNV010000105         8732 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSNV010000105 DAJSNV010000000
VERSION     DAJSNV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.109"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..74
                     /locus_tag="ON516_002409"
     tRNA            2..74
                     /locus_tag="ON516_002409"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ON516_002410"
     tRNA            78..150
                     /locus_tag="ON516_002410"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ON516_002411"
     tRNA            155..225
                     /locus_tag="ON516_002411"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ON516_002412"
     tRNA            238..311
                     /locus_tag="ON516_002412"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ON516_002413"
     tRNA            323..394
                     /locus_tag="ON516_002413"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="ON516_002414"
     CDS             660..1853
                     /locus_tag="ON516_002414"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3345867.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ON516_002415"
     CDS             1983..3455
                     /locus_tag="ON516_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3345868.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ON516_002416"
     CDS             3538..4326
                     /locus_tag="ON516_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3345869.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ON516_002417"
     CDS             complement(4393..5061)
                     /locus_tag="ON516_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3345870.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ON516_002418"
     CDS             5176..6888
                     /locus_tag="ON516_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345871.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ON516_002419"
     CDS             6891..8663
                     /locus_tag="ON516_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345872.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1945 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc ag
//
LOCUS       DAJSNV010000106         8689 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSNV010000106 DAJSNV010000000
VERSION     DAJSNV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.110"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(62..508)
                     /locus_tag="ON516_002420"
     CDS             complement(62..508)
                     /locus_tag="ON516_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3345873.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(535..711)
                     /locus_tag="ON516_002421"
     CDS             complement(535..711)
                     /locus_tag="ON516_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3345874.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(873..1301)
                     /locus_tag="ON516_002422"
     CDS             complement(873..1301)
                     /locus_tag="ON516_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3345875.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1393..2253
                     /locus_tag="ON516_002423"
     CDS             1393..2253
                     /locus_tag="ON516_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3345876.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2345..2695)
                     /locus_tag="ON516_002424"
     CDS             complement(2345..2695)
                     /locus_tag="ON516_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3345877.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2710..4437)
                     /locus_tag="ON516_002425"
     CDS             complement(2710..4437)
                     /locus_tag="ON516_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3345878.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4651..5577)
                     /gene="trxB"
                     /locus_tag="ON516_002426"
     CDS             complement(4651..5577)
                     /gene="trxB"
                     /locus_tag="ON516_002426"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3345879.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5722..7155)
                     /locus_tag="ON516_002427"
     CDS             complement(5722..7155)
                     /locus_tag="ON516_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3345880.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7155..8558)
                     /locus_tag="ON516_002428"
     CDS             complement(7155..8558)
                     /locus_tag="ON516_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3345881.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2362 a         1942 c         1473 g         2912 t
ORIGIN      
        1 ttcttttttt acgaacacaa aaaactgcgg acaatcactg tccgcagttt aaatgataaa
       61 attaagattg taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt
      121 tgactttagg cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag
      181 tttgagtgat tgcttcttga acaagttgtc gaatttcttc atcggaaagt tgttccggca
      241 tatatttttc gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg
      301 cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta
      361 gttcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact
      421 tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt
      481 catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa
      541 ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta
      601 gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg
      661 acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc
      721 ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga
      781 ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt
      841 taaaattatc aaatgttatt ttatttaaac ccttattgac aattttcaca tcttccgaaa
      901 atctcgaatc tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc
      961 ggacacatat ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg
     1021 ccgatttccc cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct
     1081 tccagtaaat cgatctctgc aaaatcatgg agattccgat aaatcgtatc ataacttacc
     1141 cctttgaatt cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc
     1201 ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc
     1261 aatgtttcta atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca
     1321 aaatgattac gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt
     1381 gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac
     1441 tctgctggag aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt
     1501 gagttcaacc tattccgcag aacgttcgtg acagatgaaa aaacattaaa gcaggcattg
     1561 caagatgcat tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc
     1621 gagataacac gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca
     1681 ccgatatcag aaattgaacg tttaaccggt atcaagccaa ttcggcaacc tggtgccctc
     1741 catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac
     1801 gatgatggga aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc
     1861 cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat
     1921 ttacctttga tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa
     1981 atcgtgggac taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc
     2041 tcttacggat taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactc
     2101 gattttgcga atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta
     2161 tctattgaag aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat
     2221 tatggaactg aaacaacaga aaaaagcgat taatcatttt tgtctttttc ttaaaaaaca
     2281 ctggagtgtg acaacgaaga aatttcgttg tcacactcca gtgtttatct atttgcacct
     2341 atcgttattg atgttccaca tgtttgagtg tctggttcaa aatatccagt acatgagaat
     2401 catcaagact atacaaaatc gcttttccct cgcgatgaga cttgacgaca tgattttctc
     2461 ttaataaacg tagttgatgc gatacagcag actgctccat ttccactgct tgggtgattg
     2521 ctgtcacatt ttgttccccg tctttcagat atagcagaat tttaagacga gtgggatcac
     2581 tcaacatttt gaataattga ctgactttct ctacttttga tttttgcgtg tctggcaatc
     2641 cattcacctc caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc
     2701 tcaagttttt tattcttctg tcatcttttt gataatcgct tcataagagg ctactttttc
     2761 ggctgcgtct tctgtagagc ttccttttac accaatataa aacttgattt tcggctcagt
     2821 tcctgaaggg cggatagcaa tccagccatt ttcttccaat gtatatttca atacgtcgga
     2881 tggcggtgtt gttagttttt ctacgtttcc ttcacggtct gttctcgtca attctttaaa
     2941 gtcttctgtt tgaataactt ttgctccggc aaattctgtc ggtgcttctt tacggaattt
     3001 ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg ctcattgtca cagaaatcgt
     3061 tttttctgca aaatagccat aggtttcaaa aatcgtttgc aagccgtcat acaacgtttt
     3121 gccttgtttt ttatagtaag ctgctacttc tgctaataaa agcaatgctt ggatcgcgtc
     3181 tttatcgcga acaaaaggtt taactaaata accataactt tcttcgaatc caaacataaa
     3241 agtatgtgaa tggtcttctt cgaattgttg gattttttca gcaatgaatt taaaccctgt
     3301 caacacattg aacatggtag caccgtagtg ttccgcaatt gctgtaggca attcgctgga
     3361 aacgattgat ttcaataccg ctgcgttttc tggtaaagtg cctcttgatt gatgtgcttc
     3421 caaaatatat tgagcaaaca atgccccgat ttggttccca gtcaatactt cataagaacc
     3481 atctggcaaa cgaaccgctg cgcctaaacg gtctgcatca ggatctgttg caatcagcaa
     3541 gtcagctcct tctttttctc caagtcgtat agcatattca aaagctgaat gttcttctgg
     3601 atttggcgat ttgaccgtag tgaaatctgg atctgccaca gcttgttcgg gaactagtac
     3661 gaattgttcg aatccagctt gtttcaacgc acgttcgccc aacattttcc ctgttccatg
     3721 aagtggcgtg tacacaagtt tcaaatcttt ccccattgtt tctaccaatt cacgatcaat
     3781 cgtcactgct ttgatttctt ctagataagc agcgtccact tcttctccga tgatcgtgat
     3841 caacccgcta tgtttagctt cttcgtcaga taatacttcg acttttagcg gattgctgac
     3901 ttcacggaca tatttcgtca atgcatctgc gtctgcagga ggcatttgtc cgccatcttc
     3961 tccgtagact ttatatccat tgtaagccgc tgggttgtga gatgcagtta ccatgattcc
     4021 cgcaaacgtc ttcaaatgac gaactgcaaa agaaagctct ggtgttggac gcagactttc
     4081 gaatacataa gacgggatat tgtgatgtgc cagtgttttg gctgcttcca ttgcaaattc
     4141 tggtgactga tggcgtgagt cataagcaat agcgacacct cggcgttttg cttcttcacc
     4201 ttgtgtatcc atgaaacgtg ctagtccttc tgttgcttgt cttaccgtat agatgttcat
     4261 tcgattgatc ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc
     4321 ataaaaagca tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa
     4381 gttttcttct aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa
     4441 aataacacct ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg
     4501 aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat tcttttatca aaaggtgttg
     4561 gacgtgttcg cataaatcag attcagtaaa tttcacgcga aaaaaaagag taaaagaaca
     4621 attgaacccg ttcttctact ctttttaaac ttatgcagtt gtttcttcta aagattccaa
     4681 gtatttatac acttgttgtc cagcaatacc gccgtcacca acagctgtag tgatttgtcg
     4741 caaatctttt tcacgaacat ctccgatggc aaaaacgcct ggcatattcg ttttcatttc
     4801 gttgtctgtt ggaatccagc cagcttcgtt cgtcaaacct gcttctctaa aaggtgcagt
     4861 taatggatcc agtccaacat agatgaatac tccgtctgct gctaaatcaa attcattgcc
     4921 ggttttgaca ttacgtgctt tcacacctgt cactaccatt tcgtttccaa cgatttcgtc
     4981 aacgactgta tcccacataa aggaaatttt ttcatttgca aatgcacggt cttggatgat
     5041 cttttgtgca cgtaattcat ctctacggtg gacgatcact acttcagaag caaattgtgt
     5101 caaatagatg gcttcttcta ctgctgagtc cccacccccg atgactacta gacgtttgtt
     5161 acggaaaaac gcaccatcac agactgcaca gtaagaaacg cctcgtccag caaattcttc
     5221 ctcgccaggt acgcctaatt tacggtggat acagcctgtt gcgataatga ctgtttttcc
     5281 ttcgtaactt ttatcttcac agatgacttc tttatggctg ccgtgatcct tgatatcttg
     5341 aacgattcca taagcatttt ctgtcccaaa tcttgagaca ctttcataca tcttataagc
     5401 tagatcgggg cccatgatcg aatcgaatcc aggataattt tcgatttctg ccgtattgtt
     5461 catttggccg ccaggggcac cttgttcgat catcaatacg gataagtttg aacgtgaagc
     5521 atataaagca gcagtcatcc cagcaggacc tgcgccaata acgattacat catacatatg
     5581 tagattctcc tttacttaat caagtaagca tactttacag ccaatgcgaa aaaaagtcta
     5641 ttgttctgct tgcgtttctc gaatagcttt attatactcg aaaaatccgc tgaaaaacaa
     5701 tagactttta taaatcattg atcaatcgtt cgaaagattt tctccatttg aatcaattac
     5761 ttttttatac cagttgaatg attttttctt tgaacgggct aatgtgccat ttccttggtt
     5821 atcacgatcg acataaatga atccgtatct tttcttcatc tcaccggtac cagctgatac
     5881 taaatcaata catccccaag tcgtatagcc taatagatcg actccgtcta gttctactgc
     5941 atccttcatc gcctggatat gcgctgccag gtaatcgatt cggtaatcat cttccacata
     6001 accattctca tctggcgtat cgactgcacc taagccgttt tctacaataa ataatggttt
     6061 ttgataacga tcataaatat cattcatcgt tattctcaag cctaatggat caatttgcca
     6121 cccccattca cttgcttgca gatatggatt tttgacagaa gcaaagatat ttcctgcagt
     6181 ctgttccaag agttcgttgt cggtagtcga aactcgggaa gaataataag aaaaagagat
     6241 aaaatcaaca gtatgttctt tcagcaatgc ggcatcccct ggttccattt cgatctgaat
     6301 gccatttcgt tccatttctt tcaatgcata agctgggtat tctcctcgtg actgaacatc
     6361 aatgaagaaa taattttctc gatctgattt tctagaagcc caaacatctt caggattgca
     6421 agtgtatgga taatatgctc ctgctgccag catacagcct actttgttct ccggatctac
     6481 ttcatgagca atcttcgttg cgatggcact tgcaaccaac tcatgatgtg ccgcttgata
     6541 tttgacttgt tcttcgtttt ctccttcttc aaagtacagt cctgctccca tgaatggtgc
     6601 atgaaggatc atattgatct cattgaatgt cagccagtat ttgacaagtc ctttatatcg
     6661 attgaagatg actcgacaca gattttcata aaaaccaacg agttctctgg aacgccacgc
     6721 accgtattct ttgatcaaat gcattggaca atcaaaatgc gtaatcgtca caagtggctc
     6781 gattccgtgc ttttggcatt ctttaaagac atcctcataa aatttcaagc cagcttcatt
     6841 cggctctttt tcatccccca ttggaaaaat acgactccag gcgatggaca ttcgataagt
     6901 cttgaacccc atttctgcaa acaaagcaat atcttcttta taacgatgat acatatcgat
     6961 tgcaattttt gctggataaa aatgatcctc atcaaattca aacatttttt tctttccggt
     7021 gataacagga aaacggtctt ttccgattgg cacaacatct acgtttgcta gtccgcgtcc
     7081 accttcatca taaccgccct cacattgatt ggctgctgta gcaccgcccc ataaaaaatc
     7141 ttttctgaat cccattatac ttcctccatt atatgtgtat tacctgataa ttctcttgcc
     7201 agttcatctt gaacttcgat tccttcttct tttaatcggc tgattgtttc cttgaattgc
     7261 ggtaaatact gctggtgtgc aataaacaac tcatccatga tccgttttgc tgtctttcct
     7321 gatcgtataa gcggattgat cgtaaacgcc tgtaaagctg tcccataatc accagtcaca
     7381 gctgcttcga tcgtcagtaa ttccatattc ttcattactt gcaaccagcc tctttcagca
     7441 gcaggaagag aaccaaacgc tacatttctc gctccttgtg cgccaacata ggcagtgact
     7501 tctactacac aatctgccgg caggtcagga acagctccat cattctttgt agataccacg
     7561 atctgtgttt ctttatttcc atagattgaa gcaatcgttt cacaggcagc gtctgaataa
     7621 taagcgccac ctcgtttttg caattgttca ggtttatggt ctaattctgg atctttgtat
     7681 aattcaaata gctctgcttc tgtttctttc acttgctgcg ctcttgtacc aatcgtttga
     7741 tattcttcta aagcatgttc cagcatttct tcttcacggt agtaatagcg gtggtagcca
     7801 caagggatca tattcatttg ttttaattgt tctttgaaaa acgggatatc aaagatattt
     7861 ttcggcaatc cattgtcttc atcgtataaa cgatcaatca attctgccgt gcggtcattt
     7921 cctttattat ctgctacttt gtgccaatgg aaatgattta gaccggcaaa cttgtagatc
     7981 agctcttctt ttttcttgcc aagcattgct ggttctgtca tcatcgccat tactggcaca
     8041 ttgcataatc caatcacttt atcccatttg ccatatctga taactgcttc tgtcaccatc
     8101 ccacttgggt tcgcgaaatt gatcagccaa gcatttggac ataaacgctt catgtcttca
     8161 acgattgcca agaccactgg aacggtacga aaagctttga atatcccgcc agcaccattt
     8221 gtttcttgcc caatcatccc ataataacta gggatccgct cgtctttgat tcgtgcatct
     8281 aattgaccga cacgaaactg agtcgtaaca aaatccgcat ctttcaatgc ttcttcccga
     8341 tccagagttg catagatttt gacttcatac ggagaagcat cccacatacg ctgagacatc
     8401 tgactaacga tctcaagctt ttcttttcct tcttctacat ctacgagcca gatttctcgt
     8461 atcggtaatt cctcatatct tttgataaat ccttccatca actcaggggt gtaactgctg
     8521 cctccaccaa tcgtagcgat tttgattcct tttgccatgt tctcactcct ttactttttt
     8581 acactttcat tatctttttt gtaaacaaaa caaacaatag aaatccgttt ctttccagta
     8641 acatttacaa aagttactgt tttctagtgg atttttgtaa atatcttgt
//
LOCUS       DAJSNV010000107         8635 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSNV010000107 DAJSNV010000000
VERSION     DAJSNV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.111"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(188..2569)
                     /locus_tag="ON516_002429"
     CDS             complement(188..2569)
                     /locus_tag="ON516_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3345882.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2559..3206)
                     /gene="recU"
                     /locus_tag="ON516_002430"
     CDS             complement(2559..3206)
                     /gene="recU"
                     /locus_tag="ON516_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3345883.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3270..3809
                     /locus_tag="ON516_002431"
     CDS             3270..3809
                     /locus_tag="ON516_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3345884.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3899..4318
                     /gene="gpsB"
                     /locus_tag="ON516_002432"
     CDS             3899..4318
                     /gene="gpsB"
                     /locus_tag="ON516_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3345885.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4340..4707
                     /gene="rnpB"
                     /locus_tag="ON516_002433"
     ncRNA           4340..4707
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON516_002433"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4916..6082
                     /locus_tag="ON516_002434"
     CDS             4916..6082
                     /locus_tag="ON516_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3345886.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6128..7624
                     /locus_tag="ON516_002435"
     CDS             6128..7624
                     /locus_tag="ON516_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3345887.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7705..8388
                     /locus_tag="ON516_002436"
     CDS             7705..8388
                     /locus_tag="ON516_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3345888.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2704 a         1572 c         1837 g         2522 t
ORIGIN      
        1 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
       61 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      121 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      181 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      241 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      301 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      361 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      421 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      481 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      541 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      601 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      661 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      721 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      781 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      841 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      901 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      961 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1021 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1081 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1141 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1201 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1261 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1321 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1381 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1441 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1501 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1561 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1621 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1681 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1741 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1801 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1861 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1921 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1981 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2041 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2101 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2161 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2221 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2281 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2341 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2401 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2461 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2521 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2581 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2641 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2701 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2761 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2821 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2881 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2941 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3001 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3061 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3121 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3181 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3241 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3301 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3361 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3421 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3481 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3541 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3601 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3661 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3721 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3781 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3841 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3901 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3961 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4021 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4081 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4141 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4201 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4261 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4321 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4381 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4441 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4501 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4561 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4621 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4681 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4741 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4801 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4861 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4921 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4981 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5041 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5101 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5161 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5221 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5281 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5341 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5401 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5461 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5521 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5581 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5641 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5701 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5761 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5821 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5881 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5941 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6001 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6061 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6121 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6181 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6241 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6301 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6361 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6421 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6481 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6541 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6601 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6661 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6721 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6781 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6841 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6901 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6961 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7021 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7081 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7141 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7201 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7261 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7321 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7381 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7441 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7501 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7561 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7621 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7681 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7741 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7801 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7861 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7921 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7981 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8041 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8101 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8161 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8221 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8281 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8341 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8401 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8461 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8521 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8581 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct ttttt
//
LOCUS       DAJSNV010000108         8539 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSNV010000108 DAJSNV010000000
VERSION     DAJSNV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.112"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(228..905)
                     /gene="nth"
                     /locus_tag="ON516_002437"
     CDS             complement(228..905)
                     /gene="nth"
                     /locus_tag="ON516_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3345889.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(912..1610)
                     /locus_tag="ON516_002438"
     CDS             complement(912..1610)
                     /locus_tag="ON516_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3345890.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1878..3062
                     /locus_tag="ON516_002439"
     CDS             1878..3062
                     /locus_tag="ON516_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR3345891.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3068..3511)
                     /locus_tag="ON516_002440"
     CDS             complement(3068..3511)
                     /locus_tag="ON516_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3345892.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3737..4024)
                     /locus_tag="ON516_002441"
     CDS             complement(3737..4024)
                     /locus_tag="ON516_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345893.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4195..5547)
                     /locus_tag="ON516_002442"
     CDS             complement(4195..5547)
                     /locus_tag="ON516_002442"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3345894.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5820..6266)
                     /locus_tag="ON516_002443"
     CDS             complement(5820..6266)
                     /locus_tag="ON516_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3345895.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6502..7428)
                     /gene="cysK"
                     /locus_tag="ON516_002444"
     CDS             complement(6502..7428)
                     /gene="cysK"
                     /locus_tag="ON516_002444"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3345896.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7568..8455)
                     /locus_tag="ON516_002445"
     CDS             complement(7568..8455)
                     /locus_tag="ON516_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3345897.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2323 a         1752 c         1419 g         3045 t
ORIGIN      
        1 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
       61 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      121 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      181 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      241 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      301 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      361 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      421 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      481 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      541 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      601 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      661 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      721 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
      781 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
      841 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
      901 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
      961 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1021 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1081 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1141 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1201 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1261 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1321 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1381 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1441 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1501 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1561 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1621 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1681 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1741 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     1801 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     1861 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     1921 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     1981 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2041 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2101 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2161 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2221 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2281 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2341 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2401 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2461 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2521 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2581 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2641 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2701 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     2761 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     2821 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     2881 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     2941 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3001 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3061 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3121 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3181 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3241 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3301 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3361 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3421 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3481 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3541 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     3601 accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag
     3661 taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc
     3721 ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat
     3781 atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag
     3841 gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt
     3901 gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt
     3961 tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat
     4021 tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa
     4081 cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact
     4141 tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg
     4201 tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg
     4261 gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt
     4321 ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg
     4381 agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt
     4441 agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc
     4501 gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta
     4561 aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc
     4621 cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact
     4681 tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg
     4741 catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag
     4801 ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt
     4861 tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc
     4921 gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg
     4981 tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc
     5041 agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac
     5101 acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag
     5161 gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt
     5221 catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt
     5281 tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc
     5341 taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc
     5401 gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac
     5461 tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg
     5521 agtacagcca attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg
     5581 acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa
     5641 aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt
     5701 attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa
     5761 gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt
     5821 tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata caattccaac
     5881 cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc
     5941 cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc
     6001 tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca
     6061 atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga
     6121 tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat
     6181 tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata
     6241 acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca
     6301 tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt
     6361 ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt
     6421 tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta
     6481 tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc
     6541 accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt
     6601 tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc
     6661 aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac
     6721 agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc
     6781 cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt
     6841 cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc
     6901 tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt
     6961 cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt
     7021 ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt
     7081 caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc
     7141 aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc
     7201 tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc
     7261 tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa
     7321 ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat
     7381 tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc
     7441 cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg
     7501 tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtaatttct ttgccttttt
     7561 atctaaatca aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc
     7621 catttttcgc cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg
     7681 tatcacctga cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag
     7741 atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg
     7801 aacctgttga tggcgtatca aaacgggtta aattataagt attaatcaat gagttcaatt
     7861 tggccccata atttggatcg gtagcataac gacctgtcaa gtacgctgtt gcatcataaa
     7921 aacttgttgt attgcttttc cacacgccag cataatgata atcgcccgtg gaaaaactgg
     7981 ttgaacgaag aacattcgca tggtcttgga aggattccca ataagaagaa tattttcgga
     8041 atggctcatt catcgttacc cattgtccgt ttacatattc tttggttgcc atataaacga
     8101 cctgtccgtt atagcttcct ttcacaccga atagattata atttggcgca ctagctaatc
     8161 cggaagtgcc atatgcactt tccaacaacg cttgagcgat catgactgaa gcatacaagt
     8221 cattacttgc agcaacttct gacgcagacc agccgatttg atcgataaac gcttgaggat
     8281 cagcgactgt ttgagatcct tgttcatctg catgaacatg ttgcggtacc gctgtaacca
     8341 tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt ttattgatca
     8401 ccatttttct tttaggtcta tggtgccggg cactgcgggt cttcagctct tgcattcaaa
     8461 ttcctcctca aaataaaaaa acttaataaa aagttctctt cttaaaacaa acttaattaa
     8521 ttaaattata cttttaatt
//
LOCUS       DAJSNV010000109         8232 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSNV010000109 DAJSNV010000000
VERSION     DAJSNV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.113"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ON516_002446"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ON516_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3345898.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ON516_002447"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ON516_002447"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3345899.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ON516_002448"
     CDS             2594..3379
                     /locus_tag="ON516_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3345900.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ON516_002449"
     CDS             3392..4243
                     /locus_tag="ON516_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3345901.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ON516_002450"
     CDS             complement(4367..4819)
                     /locus_tag="ON516_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345902.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ON516_002451"
     CDS             complement(5284..5652)
                     /locus_tag="ON516_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3345903.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="ON516_002452"
     CDS             5802..6284
                     /locus_tag="ON516_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3345904.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="ON516_002453"
     CDS             6321..7331
                     /locus_tag="ON516_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3345905.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="ON516_002454"
     CDS             complement(7446..8159)
                     /locus_tag="ON516_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345906.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DAJSNV010000110         8139 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSNV010000110 DAJSNV010000000
VERSION     DAJSNV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.114"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..730)
                     /locus_tag="ON516_002455"
     CDS             complement(<1..730)
                     /locus_tag="ON516_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3345907.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLS"
     gene            complement(741..2117)
                     /locus_tag="ON516_002456"
     CDS             complement(741..2117)
                     /locus_tag="ON516_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3345908.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2246..2893)
                     /locus_tag="ON516_002457"
     CDS             complement(2246..2893)
                     /locus_tag="ON516_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3345909.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2977..4404)
                     /gene="uxaC"
                     /locus_tag="ON516_002458"
     CDS             complement(2977..4404)
                     /gene="uxaC"
                     /locus_tag="ON516_002458"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3345910.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(4478..5557)
                     /gene="uxuA"
                     /locus_tag="ON516_002459"
     CDS             complement(4478..5557)
                     /gene="uxuA"
                     /locus_tag="ON516_002459"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3345911.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5560..7191)
                     /locus_tag="ON516_002460"
     CDS             complement(5560..7191)
                     /locus_tag="ON516_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3345912.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7514..>8139
                     /locus_tag="ON516_002461"
     CDS             7514..>8139
                     /locus_tag="ON516_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3345913.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNV"
BASE COUNT         2624 a         1624 c         1096 g         2795 t
ORIGIN      
        1 ccgataaaca tttaaataaa atatcagttc ttaagttttc ccatgattct ttttctttat
       61 taaccaaaat aggataaaag aaaactttat tcttatttgc agcttctaga tcaccaggag
      121 aatcaccaat cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa
      181 tgtcttcttt ttttccttta tcttgacaac acagttcatc aactaaggat aataattcat
      241 gtctttccca ttcttcttga atagcttcct tattagctga gcttacaata attatcttcc
      301 catatttttt cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt
      361 tagcttgact aatctgtcga ttgacttctt ccgaccattt taaagctttt aataaatcct
      421 ctgactgaga gtattctatt tccctcttca aagatggatt agacaattct tttgttacag
      481 atacccagtg ctttaatcga ttaatcgatg gtacatcatt tttttctaat gttttaacta
      541 aacctaaaaa tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct
      601 gtaggaattt ttccctatga tgaacatcaa aaatctctgc agcaattggc ccgaaaaaga
      661 gattgtgttt ataagtcatt gtatccatga cacaaccatc tgaatcaata caaaatatcc
      721 aactgttcat gttttttctc ctagttatgt ttttgattta gcaaattata attttctaat
      781 actttttctt tagataatgg atatacgaaa aataaaatca atcctacaac aaagaaacaa
      841 actgctggaa tcaacgttga agagttatac agcttttgta aaacttctcc actttgtact
      901 tttgctaacg aatcataccc tattatagaa agagtccaac ctccgacacc gccagctaaa
      961 gcttgtccaa tttttcgggc aaaagaatat actgcataaa tagttccatc ttctctttgg
     1021 ccagatttca cttcttgatc atcgattaca tcagtaatat tcgcccaaat gactgtatta
     1081 aaaataccta accccatata tccaacagaa gtaagaacta agaatacata catattacta
     1141 ggctttatca aaaataaaag taaatataca atagcagaga agaacatccc tactgacgcc
     1201 aattcttttt taccgtattt tttactgaaa attaaactta caggtgaaac aagtaccaac
     1261 atcaaaatag gattgataaa gttcattaaa actattccct gtgcattatt ataataatct
     1321 ggaaatacat aattgttcat actcattgtt aaaagctgag caagcaacaa aaaaatagct
     1381 gcagctatta tagaaagcaa cgctctattt ttagctaatc cttttaaaga tttcaacaat
     1441 gaaactttta actgcgattc atcactttta acccgttcta cagttaattt ataacatatt
     1501 acgtaacaaa taactgcaca cactgaaaat actcctgcca caattgtaaa agttgatcca
     1561 ccacgtacaa tttgagctcc agaactatct cttgtataaa taaaaattgg tgttaacacg
     1621 ccaatgaaca tacctgctaa ggtagctcct atactacgaa atgttgataa cgctgtcctt
     1681 tcttctgcaa cagaagtgat agctgatgcc attgaaccgt aaggaatatt aatagatgta
     1741 taaaaaatac ttccccatag tatatatgtt acatacatat atataacttt aatactcata
     1801 gaagctgatg ccattgatgt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc
     1861 atccgtaaaa tccacggttt aaatttgcca gttttagatg gttttgatcc atccacaata
     1921 cgccccattg ttatgtctgt aactgcatca ataaatcttg ctactaaaaa taatgttcct
     1981 acggtagctc cgcttatccc caatactttt gtataaaaaa tcattaaaaa agaactagca
     2041 aaaatgaaag tgaaatcatt cccaaaatct ccaaacatat atcctatttt gtctcttaga
     2101 ccaaattttc tttccatata atttgcgctc cttcctattg ttaagcgttt tcattctgtt
     2161 gaactgccaa cttacgtact atactctgaa gtataaagag ttagtattta ttttttacat
     2221 tttatagtat tatttatttt ttctcctaat aattctttat ctcataaaac ttatctatat
     2281 atttactagc tattcgagtt acttcttcaa aatcaccttt ttcaacagga gccaacaaat
     2341 tcccacctac accaactgca acaacgcctg cttcaaacca ctcttctaag ttatctagac
     2401 ttactccgcc agtaggcatt atatttacat atggtaatgg agcttgatac gtttttacaa
     2461 cagatggacc aaaagcagaa cctggaaata gtttaatgat ttctactcca gcggtaagtg
     2521 cctcctttat ctcagtaatt gtcatacaac ctggtaaata aggaatttga tataaattac
     2581 aaatctctgc tgtttgtcga tcaaaagatg gacttacgat atattctgct ccagccataa
     2641 ttgcaatacg agcagtaaac gcatctaaaa ctgtaccggc tccaattatt acatcttttt
     2701 gatcagcata ttttttagca atttctttga ttatttcttc agcattcgga actgtaaaag
     2761 ttagttcaat ccctttaatt ccacccgcag taattgcatt tattgcttta atggcctcat
     2821 ctttattttt tccacgtaca accgcaatga ctccagccgc ttctaactta cttaaaattt
     2881 ctactttttt catatctatc tccttctttt atggatacat actctagcta gttgtactaa
     2941 cttacaataa ataaatttaa gcttttattt tttgtattat tttctacttt cgatttcaaa
     3001 aaaattaaaa taattatacg cattacgata acaaatattc tccactatct gactaagata
     3061 catttcatca tctggaattc gacctgcttt aacccactcg cttagtaatt gacataatac
     3121 tcgacggaaa tactcatggc gaggatagct tagaaaactt cgagaatcag tcaacatacc
     3181 tgtaaaattc gacaacaaac tttgttgcgc catcgtagtc aactgttgac gcattccttc
     3241 agctgtatca ttgaaccacc aagcacatcc caattggatt ttttgaatca tacccccttg
     3301 gaagctttgc attgctgttg ctagtggaag ccaatcggtt ggatttaatg aatataaaat
     3361 tgtttttggt aagcacccac tcttttcagc agtgtcgaac aatgctacaa cattttgtgt
     3421 caaactagct tgatccccca tcgaatcacc accgacatct actccaattt gttcctttaa
     3481 tatacttgaa ttatttctta aagcatttaa atgcatttgc atcgtccaac catatcttgt
     3541 atactcagac atcaaatgta attgaatacc tgtaacaaat tgtgcaattt ctgataaatg
     3601 gaaatccgtt tttcctgcta aagcttttat taacagattg tcgagtattt tttcatctgc
     3661 tatttcataa aaataagtat tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa
     3721 ataatccact cgttggctca atgcctgctt caacccatta aagtttttga ttggtatacc
     3781 cgctgcttga ctaagtttta aaatatagtt cgaaaatcct gatgatttga tactccagac
     3841 cttatctgga cggaaagtag gtaatacctt gaaagaatta tcctgtttag aaagctcagt
     3901 atgatatcgc aagtcatctg taggatcatc cgttgtacaa atggctttca catccatcat
     3961 cttcagcaat tcttttgggc gataggaatc cgtttggatt ttttcgttgg cacgtcgcca
     4021 aatttctggt gcattcttct cagaaattgt caaatcaata tcaaagtaac gttgtaattc
     4081 caaatgagac cattcaaaaa taggattacc tggcgccttc tccactgttt ttacaaaagc
     4141 taaaaatttt tcataatcat tacctgcacc actgatcaac tcttcttcta tcccattagc
     4201 tcgcatcaat cgccatttat aatgatctcc agctccttta tcatttaacc atacctgagt
     4261 caaattcttg tattgcttat tttctagcaa acatgctggg tctaaatgac aatgataatc
     4321 gatgattggt tggtgttctg caaatttatg aaaaagtctt ttagctattt cattctctaa
     4381 taaaaagttc ttatctaaaa acatatttaa gcactccttt ccttccctta gtttttagaa
     4441 gagaaaattt ttcttctaaa tagactccat tatataatta tttactcgcc cccttttcaa
     4501 gcgcttccca aataccattt aaataaactg cacctagagc tcggtcatat aacccataac
     4561 caggtctacc ggtttctccc caaatcatcc gaccatgatc tggacgaata tagccattaa
     4621 atccattatc gtacattgtt tttaatatct tatacatatc tagagaacca tattctgatt
     4681 tatgcggcgc ttcataaaaa tcgagtccat tatcacttaa atatttgata ttacgaacat
     4741 gtgcaaaagg aatccgatcc cgttgacaaa attcagatag aatttcatag acatcattat
     4801 ctggtcgttc tgcaatactt ccagtacata acgtaatacc attagaagga gaatccacta
     4861 cattacacaa ccattctaaa tcgtcacgat ttttaacaat acgaggtaat ccgaaaatag
     4921 aatatggtgg atcatctgga tgaactgcca ttttaatccc aacttcttca catgtcggga
     4981 taattgcttt gagaaaatat gcaaagttct ctcgcaattt cgcttcatca acttctttat
     5041 aggcttctaa taattgcttg atttgtgata accgatgagg ttcccaaccc ggcaattcaa
     5101 aattattagt agaattttct acttttctaa taatttcctc tggatcagta ggaatatttt
     5161 ttgaaatata ggctaacgta gaagacccat catctaaatg ataatccaaa tctgatttta
     5221 cccaatcaaa gactggcata aaattataac agataacctc aatcccaaat tctgccaaat
     5281 tgcgaatcgt ctgtttataa ttctcgatgt attgatctcg tgttggcaaa ccaattttga
     5341 tatcatcatg aacattaaca ctctcaataa ctttcaaggt aaggccattg gcttcgactt
     5401 catcttttaa agctttaatt cgttctctcg gccagacctc tccggctggt atatccatta
     5461 atgttccaac aattcctttc attgctggga tctgacgaat ttcttgtagt gagatttttt
     5521 cttcatttgc cccataccat ctaaaagtca tttccatagt tactttcctc caacttctgt
     5581 tatatattta ttgattgttt tacgaactgc ttgaggacct actagcattt cgcaaaagat
     5641 tttttcaatt ttctttccta tctcaacttg atataaatct aaaccaaata tttccttatt
     5701 ttctaagatt tcctgtaccg cacaatgaat ctttgcagga tcttgttcac ctaatttcaa
     5761 tactgagaat ttttcctgca actctaccaa taaaggatca ggacttggca caaaattttt
     5821 tccttgatca tcgactgcca ataaataacg taaccaagca gcaatagcga taggaataaa
     5881 tgttagattt tctgcatttc cgtctttttc gacatacttt ttaattgttt caccatagcg
     5941 aatcgctact ttttgagaag tatctgttgc aattcgttgc ggtgtatctg gaatataggg
     6001 attaggcaaa cgtttattga ttacttcatc aataaattgt tttggatcaa taacgcctgg
     6061 actttcaact actggtaagc cttctacata acctatccct ttaattaagc caaccaaatc
     6121 atcatcttgc atttctgcct gaattgattt ataccccaac aaacaacctg ttattgctaa
     6181 tgccgtatgg agcggattta aacatgtcgt aaccttcatg atatctgctt tatcaactgt
     6241 ttctctatct gtcataatca caccagccgc ttccagttta ggtcgaccat ttggaaaact
     6301 atcttcaatg actagataat ggactttttc agtattggta aaagcagcga tattggtatt
     6361 tttcgcagta tgaattatat ctaagtcttc gaaacccgcc ttcaataaat ccattttgac
     6421 actttccgaa ggatttggcg taattctatc aatcatactc caaggaaaag tcactttttc
     6481 tttatccttt aaataagcaa taaatttttc agaaacgtat cctttttttt gccaagcttc
     6541 tgcaatcata aaaatgctat cttgaaaacg ttgaccattc tgtgagaaat tatcagtaga
     6601 aaccatcgca atcggtaact ctccagcatt aaacctgcta tacaacaagg atgtcacaat
     6661 actcatcgta tgttgaggat tggtaggtcc agattttata tctgcttcca ctaccgaaaa
     6721 aaattgtcct ttagaatctt ttaaactgta tcctttttca gtaatagtaa acgttgtaaa
     6781 ctgtaatgta ggatcttcaa aatatttaac cactctttga tagtccgtta tattctttgg
     6841 attgcaatat aaactatcag ctgttactgc taaaagtctt tttttgagtt caccatcttc
     6901 acccataaca atctccaaaa tatcattgtt gtagtcatga taagcttttt ctactacttc
     6961 ttcatcaaat gtctcacaca caactactcc ggattctaat tcattttgat ctgctaagca
     7021 ttgagcaatt tctgcatgaa atcctcgata aagatttcct ccaccaaaat gaagccacac
     7081 tggatgtttc tgaccttctt gtttgaccgc ttcgacatta aaatacggta gttgaacttg
     7141 cattttgttc aatccttcga tagaatcttg ccagtcttta atcaaaacca tatttacccc
     7201 tccatactga tatgttagtt tccgttacaa ttgtagtata aaatcgaatg taatcgctgt
     7261 caataagaaa aaactaaaaa tattcttttt tctaccatgt cagaattatt gtgtcttttt
     7321 ttagtaaaag cacaccaata tatatttttt agaactaact taaattaaag cttaatttct
     7381 ctttgatctt atttgagtat tttttgtttt tttaatagct agtatgttac tttgaaattt
     7441 gatattatat aacgagcata gaactaaaat atcaaattca aaaatgaaat tttgagaggg
     7501 attaaatatg acaattaaaa ctagtctcca aaatcaagct tatcaatcta ttcgtcagca
     7561 aattatctac gctgacttag aacctggaaa aaaagtatca gaaaaaaaat tggaagaaac
     7621 gctttctatc ggtcgtactc caatacgtga agcattgatt caactccgtc atcaaggact
     7681 tgttgataca attcctcaaa gtggaaatta tatctcatta atcgacttag atttagcaaa
     7741 aaatgctcgt tttgtcagag aacatttaga acgacaaatt atgctagagt gttgtgccaa
     7801 gatgaataat caaaagaaac aagttttaga aactataatt gcagaacaag aaaaagctgt
     7861 tagtcaaaag aatgctcgta gtttttttca taatgataat ttatttcata aagcttgttt
     7921 tgaaattgca gaacgggaag aagtatggca atggttggaa gataataata cgcacttgga
     7981 acgatttcgt tggttacgag taaaaacgga aggattaaaa tgggaaaaaa ttatggaaca
     8041 acatcatcaa ctatttaaag ccttgaccat gcaagaccct gaagaagcta acttcttaac
     8101 atccttgcat cttcatttaa tgcttgatga acaaaatgt
//
LOCUS       DAJSNV010000111         8100 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSNV010000111 DAJSNV010000000
VERSION     DAJSNV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.115"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..439)
                     /locus_tag="ON516_002462"
     CDS             complement(<1..439)
                     /locus_tag="ON516_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3345914.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNA"
     gene            complement(453..1703)
                     /locus_tag="ON516_002463"
     CDS             complement(453..1703)
                     /locus_tag="ON516_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCR3345915.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2081..3532)
                     /locus_tag="ON516_002464"
     CDS             complement(2081..3532)
                     /locus_tag="ON516_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3345916.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3853..4617)
                     /locus_tag="ON516_002465"
     CDS             complement(3853..4617)
                     /locus_tag="ON516_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR3345917.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4645..5343)
                     /locus_tag="ON516_002466"
     CDS             complement(4645..5343)
                     /locus_tag="ON516_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR3345918.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5355..6134)
                     /locus_tag="ON516_002467"
     CDS             complement(5355..6134)
                     /locus_tag="ON516_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR3345919.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6150..7094)
                     /locus_tag="ON516_002468"
     CDS             complement(6150..7094)
                     /locus_tag="ON516_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3345920.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7140..7919)
                     /locus_tag="ON516_002469"
     CDS             complement(7140..7919)
                     /locus_tag="ON516_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345921.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2290 a         1692 c         1316 g         2802 t
ORIGIN      
        1 tagcattagg ataaaattgt agcatagttt cccactgaac ataaaattga tctgcaaaat
       61 gatacatcaa ttttcctgtc tgagtagggc tggttacttt tgcaaacgat tcaatataaa
      121 tcagtttttt tcccatcatt tttgttatta agcagatagg gatcatagct aaaacacctg
      181 tacaaatcac gagatccggt ttttccttta tatatataat aattgatttg aataaattaa
      241 taagtatagt acacaaaaaa aacttttctt ttctatttac ttgtctcatg tagtacgttt
      301 tttcgccttt tgactcagct gaatattctg tctgttctgt tataacaaaa ctctcatact
      361 tctccatcag tggttttaac atcattaact gttcataatg ccccccagag gaagccgcaa
      421 aacatatctt tttgttcata ttaacctctc tatcagtcac gctgaaaaat atctctagtg
      481 taaacttttt ctttaatatc atccaagcag gtatcatatc gattggcaat aatagaatca
      541 cacatcgcct taaactgctc taaatcgttg ataaccttac ttccgaaaaa agtctctcca
      601 tctttcagtg caggctcata aatcaccaca gtcgcacctt tagcttttac gcgcttcata
      661 actccctgga ttgaactctg acgaaaatta tcactgttgc tcttcatggt cagccggtat
      721 ataccgatta aaactggatt ttccctgttg acatcatact gattatattc agcgtagttg
      781 taatagcccg ccatctgaag aacacgatct gcgatgaaat ccttacgggt acgattagat
      841 tcaacgatag cctcaatcaa atttccagga acatctgcat agtttgccaa caactgcttg
      901 gtatcttttg gcaagcaata acctccatat ccaaaggatg gattgttgta gtgagttcca
      961 atacgaggat cgagacaaac accattgatt atctgctgag tattcagccc cttcatttca
     1021 gcataagtgt ctaattcatt gaaatagctg actcgcagtg ctaaataggt atttgcaaac
     1081 agctttactg cttcggcttc agtgaatccc ataaaaagag tatccacgtt ctccttaatt
     1141 gcacttttct gcagaagtgt tgcaaaaaca tgagcagcat ttaacaagcg ctcatcagtc
     1201 atatcagtac caacaataat acgagatgga tacagattat catataaagc cttgctctcg
     1261 cgcagaaact ctggactaaa aataatgttc ttactccccg ttgtctcacg gactgattca
     1321 gtgtagccaa caggaattgt agacttgatg accatgatgg tatcaggatt cacgtccatc
     1381 acaacctcaa taactgactc aacagcagaa gtatcgaaat gctgagtacg actgtcatag
     1441 ttagtcggtg tagcaataac aacgaaatct gcggatttat aagcgctcct tgcatccaaa
     1501 gtagcagtta agtcaagttc tttttctgta agatactttt caatatattc atcctgaatt
     1561 ggagacttcc gtttattaat cagttcaatc ttttcaggga taatgtctac cgcggttacc
     1621 tggtgatgct gcgccaatag tgtggcgata ctcaaaccaa cataaccggt gccggctact
     1681 gcgatgttgt actttttctt cacaatatct cctctttcct ctcttaagta tggagaatgt
     1741 tactcctacg gctattcatc atgtcaaaat aacacagctt agaatgttga taaaataatt
     1801 ttccagaatg tgataaaatc tctttcattt ataaaccaca catctaattt tatctattct
     1861 ccacaagaca tgtagagtat tctttaataa caagatacga ccttactaaa tacttcaaaa
     1921 aattatcata tttaattcat aaatataaat cttcatttta aatgtatgcc caaaaattta
     1981 tctgtaatgc gtcatcaaaa tctagattcc aaatctttat caatttttat ccactcattc
     2041 taccacttta gtaatatttt ctatttataa cactttcgtc ctacattgat ccccccctct
     2101 gaagtacagt ttttactgtc ttaaataaaa ttttaaaatc taaccccata ctccaatttt
     2161 ttatatattc cgtgtcaagt tttgctactt cttcaaaatc agtaatatca cttctaccac
     2221 ttacctgcca cattccagta attcctggct taatagcaat tcttgcacga tgatgataat
     2281 catattcttc atactctgat ttaaggcatg gtcgaaatcc tataaggctc atctttcctg
     2341 ttagaacgtt gaaaaattga gggaattcat ctaaagacgt ggatctaata aattgaccga
     2401 ttcctgtctt ttttgttcca tctggaagta ttttatttcc gataattctt ggatcaaact
     2461 ccatcttaaa cataagatta ttgctcatct tattttcttt cattaattct acttttcttt
     2521 cttctgcatc catatacata cttctaaact tatacatctt gaacggttta ccattctgac
     2581 ctatacgttc ttgtgcaaag aaaattgggc ctggtgaatt aatataaata agtggagcaa
     2641 taaagataaa aataattccc gttgcaatac atccaacaat acctgcaaga atatcaattg
     2701 ttcgttttat aaatgcctgt tgagttgtca taacattgat acttgttgta agtactgtat
     2761 attctcctat acgaccaata gattgttttc ccaaagcact atctgatact tttgcaagat
     2821 tcaaatgtac agtaaggccc atttcagagc atcgttcaat aaattctttc atacacgaag
     2881 tacttttatt caaaataata aagacttcat ctacccatcc ctgacataag taatccgctg
     2941 catttttgga atcagaaaca actgtcactc cctcaacatc ttcaccaatc atgcttttat
     3001 ctatgataat gacaccattt attttataca ttttatagtt atgctctttc acattacgaa
     3061 taactacttc agcaatacta ctagatgtaa ctataataag tgatttgttc ccctcatttt
     3121 tcattttatg tttcaagtaa tgtttccata agaggcgagt tatatatgta attacaacgt
     3181 ataaacctcc catcaagaaa agaacgactc tagaataatc atttccaccc tgaacactaa
     3241 tgagatacaa acttcctaac agcaccaaca taatcgcttg ctttacagaa gaagcaaatt
     3301 ctatatagta ccctcttttt aatacacctc tatatgcttc aaacatgaaa ataactaaaa
     3361 gatctgccag ttcaatgaag actgccatgt tttgatacat tggaatcaca taaggattcc
     3421 aatctacctg gcgtaagaca tacgatagat aaaaagcaag ctggatacac aataaatcta
     3481 aaataataaa atcaatatgt ttaacccatc cccttgaact tttcttgtac attccttttt
     3541 actcccctct ttttaataaa ataaattgta ttacttgttt tctctcctaa atatctttct
     3601 gaaggattat tgactcgaca aattggcttg tggatattct tatatataga acatccgtaa
     3661 gccaacttaa aactccttaa caaaattttt ttactcatat tgtagatcaa aacaagtatc
     3721 caagaaatca tattttattt cttttttctg cttactcaac aacagctaaa agaaaaatca
     3781 tcataaacaa attcaactaa gccaacgatc cccttgtgaa atcaagtttt ttcttgattt
     3841 acgaataaag agctaaaata aaccaaactt cttctttttt acctcttcat agactggata
     3901 actgatttca tccccgttga tcagatcctt tgcgacttgt ttcatctgta ctactttttc
     3961 cttaccaaag tcctttgcaa tctgctcata gcactctttt aagtaaaagg tgcgcttaga
     4021 gaccccatgg gcatcagaag cagccatctg cacgagatta tgtttgacca tcgttttggc
     4081 tgttttctgg atgtctttgc caaatacccc aacataactt ggcgcagtga gttgggccaa
     4141 acatcccata tctaggaacg gaagcaaatg atttgggtct tcgcgaaaat gcacgttgcg
     4201 ttctggatgg acgattactg gtgtctttcc tagttctaac aactcaaaaa aaagccgttc
     4261 tgcatataac ggcacttcca gtgtcggaaa ttcgatcaga agatatgtat catctaaatc
     4321 tgtaaataat atttcatctc ggtggatgtc ttcgatgagc gttccggtaa tacgtacttc
     4381 ttgtccttcc aataacgtca aaggcaactg tcttttctct aattcggctt gtaacgaagc
     4441 gactagagaa atgacttgtg atttttcatt gctgtatttt ccattattgt ggtgtggtgt
     4501 gcataaaata tgcgtgatgc cttgctgaat cgctttttca gccattgcaa tacttgcttc
     4561 taagttttct gccccgtcat cgattcctgg taggatatgg caatgtagat cgatcatttc
     4621 tcttgtctcc tctcttctta ttccttaatt tccgtagtaa taaccagcat ctttgctatg
     4681 ttctgctccg ttgtacacaa cacctaagac tcgtgcttgt accatctcca gcagttcttt
     4741 tgctttagtc aaggattctt tacgtgacat attttcacgt acgaccaata aagtgccatc
     4801 cgtttttaaa gacatgatct gtgcatctgt tacggctact actggtggca tatcgaatat
     4861 caccacgtca aataactgtc ttgcttcgga taggatctga tccattcttg gcgagcttaa
     4921 caattctgat gggttgggtg gcttaggtcc actaggcaaa atcgacaagt tctcgactgg
     4981 cgttcgttgg atcacatctg ctacacttcc tgatgaacta agtgctgtac ttaatccact
     5041 ggcattgttc agctggaatg tcttatagac gacgggttta cgtaaatctg catcgactag
     5101 tagaactcgt tgacctgatt tagcaaaaac gactgcaatg tttgctgcag tggtggattt
     5161 cccttctcca ggacctgatg aagtcacgac aatcgttttg atttgctgac cagctgcgga
     5221 ggcaaactgg atattcgtcc ggatcgtacg atattgttcg gcgattggtg aagatgggtt
     5281 tgttaatgtg atcaaactga ctgcatgagt ttgtgctgct ttttgtttct gtgttcgtgc
     5341 catttttctt cctcctagac tcttgagcgg cttctgcgtg atggggctgt tttatcgttt
     5401 gtagcttttg gtcgtgcagg agctgttcct tttttacgga taccagcgtt caattctttc
     5461 gctgacattt ctggaactgt tcctaagatt gggaatccta atgtatctgt cacatatttt
     5521 tcatctttca ctgtccgatc gagtaattcc aataagaatg ccagacctac acctaccatc
     5581 attcctaaca ttaagccagt cattaaattc aatttattat ttggcgaaac aggtgtcgta
     5641 ttggcaactg cacctgaaat gatcgagatt ttatcaacat tcatcacatc ttttgcgttc
     5701 tcttggaaaa cttgtgaagt tgtgttagcg atctgttgag cagccactgc attcgtactt
     5761 attgcctgaa tcgagaacat ttgtgaattt tctgcctgat taactgagat ggcatctttg
     5821 atttgttcag cagtcatttt cacattgtct tctgtttcta aacgatcttt gacttcattg
     5881 agcactaaat cactgacgat catatcttta tacgtattga tcatttgcag gttcgtattg
     5941 acatcattga catttgcagt gtctgattgt ggcagtgtca cgatcaattg tgcttgtgaa
     6001 ctgtattttg gtgtcaagat gaaaaatgtt acgattcctg agatggccaa gccagcaaac
     6061 attgagatga tgattattac caagtgtttg cgtaatgttt caaaaatttc tttcaagctg
     6121 attgtctctt ccattttttc tccactttcc tatttattct ctagattcat ttgatctttt
     6181 aattcttttt tcacacgatc aagttcttga tcactgactt tttgatagga gatattatct
     6241 tgcatgaagc cttctccttg cagctgatct tgcttaatcg taccaaatgc ctcccgataa
     6301 tctaaagcaa tttttttcat ttcatcaaag ttcatgtctg ttttcatatt cgactctaga
     6361 gcattcaaaa cggattgata gttgctgaca ccgtttagac tcaatacttt tttcgctacg
     6421 ccttctacga tctttctttg gcgcccttgt ctaccgtaat caccatttgg gtcttcataa
     6481 cgcatacgag aataagataa tgcctgatcc ccgttcaatg taattttacc gattggaaag
     6541 tcatagccat cttgtgagaa agtgaggtca ttattgactt cgataccacc aactgcatca
     6601 acgagttcct ttagtccttt catattgata gagacatagt gatcgattgg gatgtctaca
     6661 tatttttgta cggtatccat cgccatggca gctccgccga atgcgtaagc gtggttgatc
     6721 ttgtcttttg tgtcacgacc tacgatgtcg acatacgtat ctcgaggaat gctgacgatc
     6781 gttgtctgtt tgtcttcagg actgactgtg gcaaccatca tcgtatctga tcttccttga
     6841 tcgacccgac ctaaatctcc agtatcaatc cccatcaata gaacagagaa agattcttgt
     6901 ttgtccagat caactggttt cgttcgttct ccttgggttc gttccactga ttcgtaagtt
     6961 ttctcaatag aatgtgagag atccagatat aattttgctc ctactcctac aattgctaat
     7021 actaatacga taagaactgc taaaataatc aaaaatattt ttttccctct tgattttctt
     7081 cttcgtcttg ccatagtttc ctccgtttgc taaccttgct tgaatcaacg ttttaactgt
     7141 caatagctta tattgctata gcccatttgc tgagcaacag tttgtgcttt tgttgcgtat
     7201 gcggctctta ttttttcatt ccggatctgc tctatttgac tgagcgccgt caaatattga
     7261 tcgtaagtag cggtagctgt tacttgaccg tctttcagca tcgtatcgaa tagctgatct
     7321 agttgcgtga cttgtgcaat ttgggtatca gcgaatccta gatattgcat aacgactgtt
     7381 ttccaagcac tatccttaaa aaagctcata ttttcacgta catgggcgac atctgcactt
     7441 gccaactttt ctttgatgat gacatcatcc acagcttgct gccagtttga tacgttttta
     7501 gtaaataatt tattgacagc cgtctggatc ttcagttttg attctgcatc ctctaactgc
     7561 gttaagactg ctttcttttc ttcttggatc gcagcagctt ctttaggtaa ttctgattcc
     7621 ttgatgtcat aatcatctgc cgaaagtttg atagaagata gtttatgtga caaatttgaa
     7681 actgtttctt ctgttatttc aggatttagg aaaatttttt cttttgtact gtacagctta
     7741 tctacttctt ttgataaaga ggtcacggtg tctttttgat tggtgatggc agattctgcg
     7801 taattgatcc gttgatgata ttgccgctga ttattgagat ggtatccaaa caagcaaatc
     7861 acaatagcgc ctgtgcctac cgccacagta gccaataact tgacgaacgg cttttccata
     7921 gattcactcc taaatattga tgatagataa gtaaagatgt gattcattca catcagaatg
     7981 agcgcttttt attttaaaag cataattaaa ctaatatatt catagaaata tttataaaca
     8041 ggttttccct atataatcat accacataca gtttttttga ctagggaaat tcctaattta
//
LOCUS       DAJSNV010000112         7896 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSNV010000112 DAJSNV010000000
VERSION     DAJSNV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.116"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            690..1658
                     /locus_tag="ON516_002470"
     CDS             690..1658
                     /locus_tag="ON516_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3345922.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ON516_002471"
     CDS             1669..2484
                     /locus_tag="ON516_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3345923.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ON516_002472"
     CDS             2552..3262
                     /locus_tag="ON516_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3345924.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..4539
                     /locus_tag="ON516_002473"
     CDS             3298..4539
                     /locus_tag="ON516_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3345925.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
     gene            complement(4597..5784)
                     /locus_tag="ON516_002474"
     CDS             complement(4597..5784)
                     /locus_tag="ON516_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3345926.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            5894..6271
                     /locus_tag="ON516_002475"
                     /pseudo
     CDS             5894..6271
                     /locus_tag="ON516_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            6319..7719
                     /gene="yfcC"
                     /locus_tag="ON516_002476"
     CDS             6319..7719
                     /gene="yfcC"
                     /locus_tag="ON516_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3345927.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            7752..>7896
                     /locus_tag="ON516_002477"
     CDS             7752..>7896
                     /locus_tag="ON516_002477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345928.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT         2474 a         1318 c         1610 g         2494 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaatgagtc aagtccacaa
     4441 tcttgtgtaa aagttcaacc tcatcaaaag cacaatgttt tgcgttgtga acctaactgc
     4501 catgcctttc tttctgagga gagaggtcag gagcagtagc gcagcgactc ttgatgtctc
     4561 tcctcagaaa gaagagactc gcacaggttc atggtgctaa atgttattcg aattaaattc
     4621 taaatatttt cgtggagaag ccagccactc agtgtctttt tccatcaaaa gtgttccaat
     4681 tatccgagtg atggaattga tatttggaaa aatccgaatc actcgttctc tccggcgaat
     4741 ctcttcgttc aaccgttcca acacattggt tgtacgaatc cttcggcgga tattttcagg
     4801 aaaagacaaa atttgaatag catcttcaaa gccgttttct aaaatgtcgc aagcagcact
     4861 taattttgaa tcacaaccat atttttccaa gaagtgctct tttctttcac gtgtcaattc
     4921 aagttcagag gctttaaaga tactcttcaa ctcgtctttt acatcagaag aaactttctt
     4981 tggcagctta tcaaaaatat ttctcaaaaa gtgggcttgg catctttgcc agctcgcacc
     5041 taaaaagttc ttgcgaacag ccttaactaa ccctttgtgt gcatcagaaa tgaccagttt
     5101 tacaccgaac aaacctcgtt gaacgaacga ttgaaaaacg gtatcccaat tatcttccga
     5161 ttcgccgtct tgaacatcaa aaccaagaat cgtacgtcgt ccatcagaat caatgccgat
     5221 accaatatat acaccttttg agacaatacg atgattttcc cttactttca tatacagggc
     5281 atcacacatg aaaaatggat attccgagcc ttctaggctc cggtttctcc actcaaaaac
     5341 aaatggatcc aactgtttca tcagatttga aacgaatgat ttagaaacag tcgcaccatc
     5401 cgttagcagt tcaactgttt tagttacttt gcgagtggag acaccagaaa tgaccatttc
     5461 aatcatggca gcgatcaaag ctttttcgct tcgctgatat ctttcaaaga tagtaggact
     5521 aaacttaccg tctctggttc gtggaacatg aagttctaac gtaccaattc gagttgtaaa
     5581 cgaacgatcg tagtaaccat ttctttgtga ttgccgttct tctgaacgtt cataggcatt
     5641 tgcctgaata tattgatttc gttgttcttc catcagctga ttaaacatga tagtcataag
     5701 tgtttttgcg gtatcattcg caccgctttc gtttataatt gcttgaattt cttcgctttc
     5761 aaaagtaaaa tgtacttgag tcattgatat cagtcctatt cgagtatgtt ttctgcaaaa
     5821 aacattgtac tcctaactga gatcaatggc ttttttgatg cttcactttt acacaattat
     5881 atggacgtaa tcaaaaaatg tttccacaga ggacttcttt aattattgtc gattagagga
     5941 aagtggtgta acagtcacta gcggtacaga tttaccgctg tttattactg atattcctga
     6001 atcgattata agagcagtat tccgtcgttt tcctgaagga gaagaagtct gggagaaaga
     6061 aacaagcttc acgacaaccg agctgctgaa aagttttaca agcaacagct tcaaagtaaa
     6121 tggtttatcg gagtacggag aaataaaagt agggaaaagc gccagcttag ctgttttcag
     6181 cgaggacctc tgtactgatg actggcaaaa agtgatggct gcggaagtta tcgctaccta
     6241 tcttgatggt gacttagttt acaagaaata atgcttttcg atataacgtt ataaaacgaa
     6301 ttaaagagag gttaaaaaat ggaaacaatg aaaagaaaat tcaccttccc acatagttat
     6361 acattgattt ttatcttaat tattttggct gcaattttaa cttggataat tcctagtggt
     6421 cagtttgaga cagttgttga aaacgtcgca ggtatggaaa aaacaacggt tgttccggga
     6481 acctatcacc aaatcgataa ggcagattat ggacaaggtt tttcctctat actaatggca
     6541 ccagctgaag ggattatttc agctgttgag gttgttgctt ttgttctcat cgttggtggt
     6601 gccttcggga ttatcctaaa aacgggagcc atcgatcgag ggctgtttac cttagcaaaa
     6661 gttctgggag acaaggggat cttagttatt ccgatgtcga tgattctatt cagcttagga
     6721 ggatcaacct ttggcatgag cgaggaagtc attcccttat ttgcggtgtt tatttctctg
     6781 atgttttctc ttggctatga ttccatgact gcgatcttga ttctattttt aagtacacaa
     6841 gcgggatatg ttgggtcgac gattaatccc ttcaacgttt taatcgcaca gggagtagct
     6901 ggcgtttacg gaaatcccct gctttggttt cggatgctat cctggattgt cattactggg
     6961 atttcaattg ttttcaccat gctttatgct agcagagtca aaaagaatcc actgtcctct
     7021 attgtttatg agagtgattt aaaggttaag cataagtttc aaaattttga cactgaaaaa
     7081 gagttttcaa cacaagataa actaattatc gccggtttta tcgtaggttt aggaatcatg
     7141 atttggggta tcatttccaa gggatggtat atggttgaaa tttctgcact attcttagga
     7201 ttaggtcttt ttgctggggg catcgctaga ttttctcaaa aggaaattgc tgaagctttt
     7261 gtcgaaggct gttcagattt cgcctatgct gctgtaatca ttgggctagc tagaggagtc
     7321 ttggtaatct tggaaggtgg aatgattatt gacactattc taaacagctt ggctaacctt
     7381 ttaggtggtc ttccaaaatt cctgttcact acgattctac tcatttctca agtaattgtt
     7441 tccttttttg ttccatcctc ctcaggagct gcggcattaa cgatgccggt tatggcacca
     7501 ttgggtgatc taatgaatgt aagtcgtgat gttgttgttc taagtaacca gtttggaaat
     7561 ggtttaatga atattatctc cccaactagt ggtgtattac tggcaggatt agccatcgca
     7621 gagattaatt tctctaaatg gtttaaggtt ggcttcaaaa tatttgccat cttaaccctt
     7681 gtttctgctg ttttgttata cattgctaca ctcttttagt cttaatcatc cattaatagg
     7741 aggcaatagt tatgattaaa ataaatcaat tagcaggaat gaatatccac tatttatttt
     7801 attctctcaa agaatttttc gcttatcaac agaaaattgg tatttcttct gttgaacttt
     7861 ggggaggtgc tccgcatttt ttcatagatg ctttta
//
LOCUS       DAJSNV010000113         7810 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSNV010000113 DAJSNV010000000
VERSION     DAJSNV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.117"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(234..719)
                     /locus_tag="ON516_002478"
     CDS             complement(234..719)
                     /locus_tag="ON516_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3345929.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    809..1154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1199..1840
                     /locus_tag="ON516_002479"
     CDS             1199..1840
                     /locus_tag="ON516_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3345930.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1859..2494
                     /locus_tag="ON516_002480"
     CDS             1859..2494
                     /locus_tag="ON516_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3345931.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2512..3327
                     /locus_tag="ON516_002481"
     CDS             2512..3327
                     /locus_tag="ON516_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3345932.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3646..5082
                     /locus_tag="ON516_002482"
     CDS             3646..5082
                     /locus_tag="ON516_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3345933.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5271..7718
                     /locus_tag="ON516_002483"
     CDS             5271..7718
                     /locus_tag="ON516_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3345934.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         2617 a         1314 c         1738 g         2141 t
ORIGIN      
        1 aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt
       61 ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt attagttttc
      121 agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt
      181 gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa
      241 ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc
      301 ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata
      361 gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc
      421 actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag
      481 cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag
      541 atgacaagat agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga
      601 ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga
      661 aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc
      721 gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata
      781 atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag
      841 gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct
      901 tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt
      961 cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga
     1021 accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg
     1081 gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat
     1141 aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat
     1201 ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt
     1261 atttttattt accttgattg gctcattacc attagggatt gttttagcat taggattgtc
     1321 tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac
     1381 accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt
     1441 tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga
     1501 gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt
     1561 gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt
     1621 tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat
     1681 attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt
     1741 actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt
     1801 aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat
     1861 gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa
     1921 tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac
     1981 cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga
     2041 cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt
     2101 ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa
     2161 aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc
     2221 attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca
     2281 gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc
     2341 aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa
     2401 agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg
     2461 tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa
     2521 accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa
     2581 aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta
     2641 gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat
     2701 attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt
     2761 gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc
     2821 tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac
     2881 gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat
     2941 aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa
     3001 gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca
     3061 ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac
     3121 tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag
     3181 agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat
     3241 gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt
     3301 ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca
     3361 gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg
     3421 gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa
     3481 atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag
     3541 cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta
     3601 ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa
     3661 cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa
     3721 ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct
     3781 ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa
     3841 gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg
     3901 acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag
     3961 agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg
     4021 gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa
     4081 gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg
     4141 attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg
     4201 ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat
     4261 gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt
     4321 gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat
     4381 gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt
     4441 gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca
     4501 gaattgctag aatctgctaa accagatttt cttgggatca attactatca aacggcgacg
     4561 aatgcattca atccattaaa cggagttggt gcaggaaaaa tgaatacgac aggaaaaaaa
     4621 ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc
     4681 gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt
     4741 atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac
     4801 aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac
     4861 gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg
     4921 tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat
     4981 gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac
     5041 tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa
     5101 gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa
     5161 aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt
     5221 tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa
     5281 agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc
     5341 aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct
     5401 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
     5461 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
     5521 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
     5581 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
     5641 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
     5701 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
     5761 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
     5821 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
     5881 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
     5941 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag
     6001 aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg
     6061 aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt
     6121 ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga
     6181 ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat
     6241 ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg
     6301 attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg
     6361 tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag
     6421 gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg
     6481 tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac
     6541 cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt
     6601 cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt
     6661 taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac
     6721 tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta
     6781 tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg
     6841 aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa
     6901 aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg
     6961 cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg
     7021 aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg
     7081 atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg
     7141 ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca
     7201 caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag
     7261 attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc
     7321 tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc
     7381 acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga
     7441 aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc
     7501 tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc
     7561 aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc
     7621 atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag
     7681 aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga
     7741 aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt
     7801 tccatctttt
//
LOCUS       DAJSNV010000114         7758 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSNV010000114 DAJSNV010000000
VERSION     DAJSNV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.118"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..59)
                     /locus_tag="ON516_002484"
     tRNA            complement(1..59)
                     /locus_tag="ON516_002484"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Ile,seq:cat)
     gene            complement(256..1275)
                     /locus_tag="ON516_002485"
     CDS             complement(256..1275)
                     /locus_tag="ON516_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3345935.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1272..1496)
                     /locus_tag="ON516_002486"
     CDS             complement(1272..1496)
                     /locus_tag="ON516_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3345936.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1493..1786)
                     /locus_tag="ON516_002487"
     CDS             complement(1493..1786)
                     /locus_tag="ON516_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345937.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1823..1960)
                     /locus_tag="ON516_002488"
     CDS             complement(1823..1960)
                     /locus_tag="ON516_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3345938.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1962..2393)
                     /locus_tag="ON516_002489"
     CDS             complement(1962..2393)
                     /locus_tag="ON516_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345939.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2407..2901)
                     /locus_tag="ON516_002490"
     CDS             complement(2407..2901)
                     /locus_tag="ON516_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345940.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2901..3350)
                     /locus_tag="ON516_002491"
     CDS             complement(2901..3350)
                     /locus_tag="ON516_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345941.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3354..3974)
                     /locus_tag="ON516_002492"
     CDS             complement(3354..3974)
                     /locus_tag="ON516_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345942.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3974..4882)
                     /locus_tag="ON516_002493"
     CDS             complement(3974..4882)
                     /locus_tag="ON516_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCR3345943.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4895..7657)
                     /locus_tag="ON516_002494"
     CDS             complement(4895..7657)
                     /locus_tag="ON516_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345944.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            complement(7654..>7758)
                     /locus_tag="ON516_002495"
     CDS             complement(7654..>7758)
                     /locus_tag="ON516_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3345945.1"
                     /translation="LFLDFPFLYPGKNEIKWTKNIHSFEIMPRWWRKV"
BASE COUNT         2200 a         1654 c         1162 g         2742 t
ORIGIN      
        1 tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctgacc aactgagcta
       61 agggtactgg ttgttgccac ataaagccat aaacaatcaa ccagtagaat gtgtggcaac
      121 aaacctgtta tcgcatagct tggagtgtga ctattatggg tgatagtgaa gatatgcgat
      181 aacatcacta ttttatcgaa tgatttttat agttgtcaat atagttatgt aatgctcctc
      241 aacgaggagc tatttttatc gtttaggaat atttaaatac caacgtttgt catggaaatc
      301 ttgcgcaccg cctttagtgt ttccctctgg atcattcgtt gcccgcatca tgacatagac
      361 tttcttatta gggaagttac gcatgttaaa agatacatga tagccaacat ttccagaagt
      421 attataagct tgatttacat ctgatctata aattccatca gctcttactc gagctaattc
      481 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag caatgtgcca
      541 tccagctaca tgcaaatttg cattttcgat ttctccaaac tgatcaatgt gggcgtaatt
      601 cgttccatct gtcagtgtag gattagctgc accagctcgt gttggatcaa tgacaggctt
      661 gttttcagaa gttgttggat tttcatcggt aaatccatga gctaaatcat atgctaattt
      721 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccagat
      781 gttttgtgtt acccataaat gcgatttgat tcccggttgg ttataaggag tgtccaacgt
      841 taatggaata ccatatttca ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt
      901 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactgttggc
      961 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
     1021 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
     1081 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tatactgatt
     1141 atttgctact tgtgagctac cttcatttgc gcccaaatta aattcattgt tgatagtata
     1201 ggcaaatccg ttaattggca ataaaaaaag agccgttaac aggctcatcg cagtaatagt
     1261 aattttcttt ttcatttgtt tcctccttct tcgctttcag ccgagaacat tttgtaggtt
     1321 cgatttgata cacccaacac actccctaaa aacgcgccaa aaccagtaat gatgacaaca
     1381 cagatatctg tgtactgcca attgagcgct ttaccaacta accccacgaa agtagctagt
     1441 gcgggaataa ttaccagtgc gaaccatttt agtacttcga acgttttatt attcattttc
     1501 ttctctccct aaacaaagtt ttgatttgtt gcgtgtgttc caccaatttt tctgtatgtg
     1561 tatctaatct ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc
     1621 tcgattctaa atctttagtc agcaaatcta aattgtggct tacttttgaa agagtctcag
     1681 taattttcga gaaagatgca gcaatcggtc taattactaa taaaatcaaa gaaacgatag
     1741 cggttattga tcctgctatc attccccatt cccctaaatt aatcatgtga caactccttt
     1801 accttaaata aaaacgcatc aattaagatg cgctcttatc tttattaatg attttatctg
     1861 cttcttcgtc tgtaatgcat aatggaacga aaaccattac ttgttcgtta gtgaaacagc
     1921 cccagtcata cattagtttg atatcttcat atgaatacat gttacttccc accttgtaat
     1981 tgttttttga tttcttcgat ttccttggta ttttgtacag aagcaagcat cgtttttgaa
     2041 ttcaactgcg ctaaactttc tgacttttct ttcaataaga cattttcttg cttgattgct
     2101 acattgttta gcattgcttt agaatttagc tgtttcaatt catcattttc tgccttcagc
     2161 gtttcgtata atgccttaat attagccaat tcatctacgc tatcaacagc attccctttt
     2221 ttctcttctt cagtagctaa ctcaacccac tgttttttat caaaatcaaa tcttggtctc
     2281 cagttaggaa ctggtggttt aatctctgta cagttttcag ggatattttc ttgattgttc
     2341 aaaatgattt gctcgaatcc gtaaggcttg attgatttgt aaactacttt cattagattt
     2401 cctcccttaa attgaataag tgataacaaa tgaataagct gatccataac ttgaatttct
     2461 tctccattta atggctccgt ctgcgccaat agatagttga gcactgttca aagtagaacg
     2521 gtctattgac ccaaccagtt gttcaaaact aattggtgtc cgatagcctt ctggaacagt
     2581 taaaataacc gaatcattcc cgccactact ttttccgttt aaagccacaa aatatataga
     2641 aactgttttt ccttcacgat aaagctttgc tgtccctgta ttaccgttag tgacaaccag
     2701 atttaccact tcatatctgt tatcatcagt cgtcaaaaca cgtttattat tgattgttac
     2761 agaacctgaa aaattcttat gtcctgcaat ggcttggtct ccatctgtct gcaccaatgc
     2821 ttctttacca tcaatagctt ttacatgcgt ttttaagtat ttagcaactc cgtcttcttt
     2881 taattgaaca atatcagcca ttaaaccgtc cccactttct caaatgtaat attggctaat
     2941 ccatcaagtt tcactttatc ggttgctgac attaaacctg ctgtcgtagt tgtagcatta
     3001 cctggatttt tctgtgctcc agctgcaatt ccatccaact tagttttatc tgtggacgac
     3061 atcaacccat ttgctgtagt tgtagctaca gctgtagttg tggcatttat tccaggatca
     3121 cctttatctc cctttggtaa aacaaaatta aatctagctg cagatgatgt tcctacattc
     3181 gtaacagaag cggttgaacc actagaaacg gttcctatgg taattgttgc tgcttggcca
     3241 ggatcgcctt tatctccctt caccgttgtt ggtttgcctt ctatagcatt ccaatgagtt
     3301 tgtggataaa cctgtacatt gctttgtttt atttttacga tatctgtcat tctctatacc
     3361 tccccaattt tttcaaaagt aaaatttgga attctttcgt ttgtgtaatt ttctgcttga
     3421 tttacagctt cttggaattt ttgatctaca tatgactgat tgacaccacc agtcccacta
     3481 ccacctgtag aactaattgt tccatcttca tcaatagtga tgttttctcc agcagtataa
     3541 tttttcagtg aatcgagttt ttcttttaac tccagcgaaa aattgacatc agattgtctg
     3601 actgcagaga ctttccctgt ctcatcaatc gtcaataatt caccaacttt tatgccaccc
     3661 aaacggtcga tagtggcaac tggcaacacg tagtcacctt ctccactgtt taaaattttt
     3721 tgatacattt ctgccgtgat aatgccgtca tcttcttcac ttgcataggg taattctgtg
     3781 agaacattct ctaatcccaa atcttcttta gtaatgacga cggctccaat ttttccatta
     3841 acagataaaa ctgttgcttc tcctgctatt atttcttcta acccaagaac tgcggaagca
     3901 tgtgtgattg gaaagaattg gcgttgtaca cctgtttttt catccgtttc catcattcgt
     3961 ttagttttag ccattagatc accccgactt tctcaagagt gaacacattt tgctttggat
     4021 catcaacggt tgcgatgatc aaggctcctt cttcaatcgg gaattctaca gttccaactt
     4081 tttcgacttc gtgattatct gaaaagagat cgtcttgaag aatatcagtc acttcaattt
     4141 caccgtattc aatcgtgaaa agtgctgctt tcattttttg atacaaataa tttagatcgg
     4201 ataggaaacg ttcagaaatc gattcatggc gtacaccttg aagatctaca cgtgcatcca
     4261 ttaattcggc taacattact ccacctggat cgatagactt caaaatatcc ttgattgatt
     4321 caaaccatga caaataatct gattcttgtc cttctctcca atcttggaaa ctattctcct
     4381 gttcttctct ccagcgatca aattcttctt tcctttcatt catccagtcc gtaaaatcgc
     4441 ctttattttc attaataaaa tctgtcatat cagcaattaa atcttcaatt gattgccaat
     4501 aagaacccat ttctccttct gtttttgaaa cagcattgat gacaaaatag gagaagtctt
     4561 gagttgttcc aatcaagtct tctcctttaa atataatgaa attggctgtt tgtcgatgca
     4621 aacactgcat ggaatattta tcaaaaatat atttgatttt ccctttttta gcatccacaa
     4681 ttcttgtttc taattggact ggatattttc cgccaacaac tgattcaaaa tataccttac
     4741 attctgataa atcatatgga agaccatttt cgacaattgt agcttccata acttcagtgt
     4801 ttttattgcc ttgtcgaact tgaatcatcc ccacgtaatt atagggttct gttgtactta
     4861 atatgacatt ccactttgcc attaaatcac ctccctattt tggtggtatg acaatggaag
     4921 aaattgcact tgcactataa tactggcgat ctaactttcc acaaatcata cctaactctg
     4981 tgttttgttc ataagtttgc atacgaccat tagctaagcc tcggataacg ccagtatgtc
     5041 cataagttcc gtctgcaaac catgaaccca cttgtccacc tcttgcccaa ttaataattg
     5101 caccaactac taattgatca tatctagggt tttgaatcac tttccaacca acagcagacc
     5161 aatcataggc aataccaata tcagatgctg cagaagtatt tcctatcaca tgagttaaac
     5221 catattttgt tccagcaccc atgccacaac cacccagata tcctgaatat tctgcagaca
     5281 agccataaca ctgaccattg ccaattcgtt gtccaatcaa agactctaaa tgttttagtc
     5341 ctgcttctcc agtagctcct cctggtttta aatctttaaa tttgttatac cagttaactg
     5401 cataatcttg acgttcagga tgtgtcgctg ccggacgttc atagtttcgt tcaaaagcat
     5461 atgctgcttg tcttggatca gtacaggcct taaatccatc aaccgtcgta ggttgtacta
     5521 cgcccatcca ttgtccattt gtaaatgtcc aaataagcaa tcgaacttgt gcatctaagc
     5581 tcgttattgg ttctttaata cctgcagcat taaataagtt ttgaacataa acttttccat
     5641 cccatgttgc tggaccaaca agaggatatg aagaaccatc ccactgaact aatccgtatg
     5701 ctggtccacc tatttgaaca gtatcaggat caaaagttcc tcctgtttct tgttgaatgt
     5761 tccccaatat tccacatgca gattgtttcg taaatccgtt attacacaaa atatcgtaaa
     5821 ttctccaagc tctcttttct gcatctgttt ttaattcact aggataccca ccagtagatt
     5881 ctccacctcc cgatggacca ccaccttgtc caggaattat ttcttttcct ttaactgtaa
     5941 gttttccttg tacatctaag tctccaaaat aaattgcttt accatttcct aataaaacta
     6001 atccttttcc tactttagga gaaatcaaaa tatatttgcc gtctccattt gtacgaataa
     6061 ctaaagaatt atcttcaata ggggttggag tagaagcccc aggaaaagga ttaccagcag
     6121 aatcagttgt tccaatcgtt ccaattgaac ccttagaatt ccaaaattcc attccttttt
     6181 tagttaactc cattattttc tttttgttgt tccaaatttg gagtagtcca ttaactaatt
     6241 tcaatacatc tccagtcttg ttaaaagaat tttgaaagat atctgctttg attaatccag
     6301 tttgtataaa attggcatta aatatactat ctaatgtcca agctgaagta aaaggccccc
     6361 gccatccttt cttagaaaaa gcaatcccat ttttattcat tcttagtact tctcttgcct
     6421 tttctaaatc tggattatcc ataataaaga tgttggaagg tttttctttt ggccataaac
     6481 ttacatatcc tcctgcactg ccttgacctg taatcattga agaaacataa tcattaaaat
     6541 cactcatata attattcgtt gcataatctt ttaatttatc ctgaatagtt acagcttgtt
     6601 gctgataaaa agcaacttga atgtctcctg cttctaattt caaagttttt tcagataaag
     6661 aatcgtattg tagtccacta acctttgatt caagataaat attatatttt ttatgatata
     6721 ttttaaatgt atcaaacaga ccataatttc taatcttcgc aaattctttc gcttcttcac
     6781 tatcagtcaa cttatcaaat tcaaccgtaa tagaaacttt aggcttatca cagccaggat
     6841 tgatcgtttt aaagtaattt ttagctattt tatttaagct cttaatgtct tttactcctt
     6901 gttcttctgt aaactgaaca tgctctgtat acacatcagg atagttatta atataggcac
     6961 tatctactgg cgaaccataa attcgattag tcgtacctac ctcactttga ggatcagcat
     7021 atggaataag tcttgttttt atacctgtcc aatctaattt gacctttaaa ccagacatat
     7081 cttttccata ccgaatcgtt ccaacattat tgcgacctct tcgctttaac aaagaaagct
     7141 taaatggttc acgtttaatt tctccacccc aatattgaag caatgatcct tgttcaccag
     7201 caatacaatt taaaacattt ctcgcttcaa agattgtgct agaaacggcc gtaatatcag
     7261 aataaagtcg tatgtcagat tttttatcca ttttggtttc aataattgat atcgcttctt
     7321 gaccagtttt agagtctact tccgcaagag tgactacccg tcttccaagt cgatttgtac
     7381 gactctgagc ataaatagtt actgtattta aaaaagtatc tatatcttta tcatcaataa
     7441 aaaagatatg atactcttct tgatcgtttg gctttgcttt tatttgatag tcattttcaa
     7501 aatattcatc aaatctagtt cctaaaggat aatccaactc taattcatat tttccattag
     7561 ctacttcata tatttcacat cttgtacaat cctttaaaat tcctaagcca ttcgttgaga
     7621 aatctgtttc agtaggacta tatattcttg gtttcatact tttctccacc acctaggcat
     7681 tatttcaaat gaatgaatat ttttcgtcca tttaatttca ttttttccag gatataaaaa
     7741 tggaaaatct aaaaataa
//
LOCUS       DAJSNV010000115         7600 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSNV010000115 DAJSNV010000000
VERSION     DAJSNV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.119"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(943..1356)
                     /locus_tag="ON516_002496"
     CDS             complement(943..1356)
                     /locus_tag="ON516_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR3345946.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ON516_002497"
     CDS             complement(1812..2297)
                     /locus_tag="ON516_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345947.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ON516_002498"
     CDS             complement(2436..2651)
                     /locus_tag="ON516_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR3345948.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ON516_002499"
     CDS             2875..3675
                     /locus_tag="ON516_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3345949.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ON516_002500"
     CDS             3694..5562
                     /locus_tag="ON516_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3345950.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ON516_002501"
     CDS             5555..6046
                     /locus_tag="ON516_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3345951.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="ON516_002502"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="ON516_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3345952.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ON516_002503"
     CDS             6606..>7600
                     /locus_tag="ON516_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3345953.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAJSNV010000116         7537 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSNV010000116 DAJSNV010000000
VERSION     DAJSNV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.120"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            107..1248
                     /locus_tag="ON516_002504"
     CDS             join(107..335,335..1248)
                     /locus_tag="ON516_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3345954.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ON516_002505"
     CDS             1483..1935
                     /locus_tag="ON516_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345955.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ON516_002506"
     CDS             1949..2638
                     /locus_tag="ON516_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345956.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ON516_002507"
     CDS             2763..5114
                     /locus_tag="ON516_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3345957.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ON516_002508"
     CDS             5194..5589
                     /locus_tag="ON516_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345958.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ON516_002509"
     CDS             5914..6531
                     /locus_tag="ON516_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3345959.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ON516_002510"
     CDS             6572..6871
                     /locus_tag="ON516_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3345960.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ON516_002511"
     CDS             6905..7072
                     /locus_tag="ON516_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3345961.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ON516_002512"
     CDS             complement(7117..>7537)
                     /locus_tag="ON516_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3345962.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAJSNV010000117         7280 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSNV010000117 DAJSNV010000000
VERSION     DAJSNV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.121"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ON516_002513"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ON516_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3345963.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ON516_002514"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ON516_002514"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3345964.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ON516_002515"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ON516_002515"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3345965.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ON516_002516"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ON516_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3345966.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="ON516_002517"
     CDS             complement(5579..6346)
                     /locus_tag="ON516_002517"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3345967.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ON516_002518"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ON516_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3345968.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1876 a         1616 c         1279 g         2509 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgccagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg atgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
     7261 aatataaatt cactttttta
//
LOCUS       DAJSNV010000118         6854 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSNV010000118 DAJSNV010000000
VERSION     DAJSNV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.122"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..1178
                     /locus_tag="ON516_002519"
     CDS             24..1178
                     /locus_tag="ON516_002519"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3345969.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ON516_002520"
     CDS             complement(1200..1952)
                     /locus_tag="ON516_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3345970.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ON516_002521"
     CDS             complement(2316..4982)
                     /locus_tag="ON516_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3345971.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="ON516_002522"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="ON516_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3345972.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2024 a         1414 c         1248 g         2168 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatccg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaa
//
LOCUS       DAJSNV010000119         6804 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSNV010000119 DAJSNV010000000
VERSION     DAJSNV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.123"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            38..928
                     /locus_tag="ON516_002523"
     CDS             38..928
                     /locus_tag="ON516_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3345973.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1001..1825)
                     /gene="nadE"
                     /locus_tag="ON516_002524"
     CDS             complement(1001..1825)
                     /gene="nadE"
                     /locus_tag="ON516_002524"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3345974.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1837..3309)
                     /locus_tag="ON516_002525"
     CDS             complement(1837..3309)
                     /locus_tag="ON516_002525"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3345975.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3946..4308)
                     /locus_tag="ON516_002526"
     CDS             complement(3946..4308)
                     /locus_tag="ON516_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3345976.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4531..5283
                     /locus_tag="ON516_002527"
     CDS             4531..5283
                     /locus_tag="ON516_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3345977.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5423..6346)
                     /locus_tag="ON516_002528"
     CDS             complement(5423..6346)
                     /locus_tag="ON516_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3345978.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6361..6633)
                     /locus_tag="ON516_002529"
     CDS             complement(6361..6633)
                     /locus_tag="ON516_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345979.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2082 a         1352 c         1161 g         2209 t
ORIGIN      
        1 ttaatatata agaaaaacct tttaagaggt gatatatatg caactacaaa taaacgaaga
       61 atccttaccg gtatatgaag cattagctag taaaacaagg atcaagatca tccagctact
      121 ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa cttggagtga gcagtgctat
      181 cacgacgatg cacgtgaaaa agctagagga agcaaacatc atcaaaacag aaaaagtagg
      241 acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat
      301 attcaatgct tttgatacga aagaaacatc gatacctatt ggtcactata caaattatgc
      361 aattgaacca acatgcggtt tagctacgat ccatgatttt atcgggaaag tggatgaacc
      421 acgctatttt atggatcctc gaaggatgga tgcccgcatt ctctggttta ccagcggatt
      481 tgtcgagtat caggcaccta attttttaaa tacagaggat acattggaga tgttggaagt
      541 ttctgtggaa atttcttctg aatttccttt ttcaaatgac aattggcctt cagatatcac
      601 tttcagccta aatggtgtgg agctaggaac atggacgagc cctggagatt ttgctgatat
      661 ccgcggtaaa tatacgcctg attggtatcc agataatctt aatcaatacg gcttgctgaa
      721 gacgatccga attaccaaac acttgacgaa tatgaatgga gaacccttgt caaacatcac
      781 gatcaacgac atccctaaag agcaagacac ttggcatttg cgaatcgaag tgaaagacga
      841 tgccaaacat gtggggggct gtactttatt tggtaaagga ttcggtaatt acgatcagga
      901 tatcaaatta aaagtttact acagctgata tttttctttt ctattttata aaacaaaaac
      961 agccaatccc tatgaaggga cggctgtttt tgctttattg ttatttccag aaatcatcca
     1021 aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt
     1081 cttgtgcttg agcgggaact tcttttcctt ctagataatc atcaatttca ttatatgtga
     1141 cacctagtgc gacttcatct gcaatcattg gtttcccatc ttcaagatct gcagtcggaa
     1201 tttttgtgta taatttttcc ggtgcattca atgcttgaag caattgtttc ccttgtcgtt
     1261 tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc
     1321 cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc tcctgctctt tctccagcta
     1381 cggcatattg cgtaatcata cgttgacgtg ctttgatatt tcctttgtta aaatcgctga
     1441 ttcccacacc tgcttcgctt aatacttgta cttgtgcatc aacagctggt ttgatattta
     1501 ctcgcagact aacatcgggc tggataaagt caagagcagc ttttgcatct tcttcatctg
     1561 cttgttcacc ataaggaaga cgaacagcga taaattgata gttttcatct tttgtttctt
     1621 cacgcatttc ttccatcgtt agttgcgcca aacggcctgc taaagtagaa tcttggccgc
     1681 cgctgatacc tagtacaaaa gttttaagaa acggatattt tttcaaatat gctttcataa
     1741 agtcaatact tttacgtatt tcttcttttg gatcaattgt tggtttgaca cctaattcat
     1801 tgataatttc tgtctgcagg ctcatttatt ctcctcctat gcttctgtat gaagttctgc
     1861 tacgcttttt ttcattcgtt ccatgatagc cattttatgg ttccagcatt cagtagacag
     1921 atcgactggg tatttttgcg gattcaagtc tcgtttatac tcttcccata gtgaatctag
     1981 attttctttt gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc
     2041 ttcgataaag atatcttgta aaactgggcg agctgtaaag tctcgaacag ttttattgat
     2101 aaacgtatgc actggatgga acatgaagat agcttcttct tctcgagggt cttcatccca
     2161 taatgtcacg tagtctcctt cagatttccc atcaaaattt cgagtgatgc gccacacttg
     2221 ttttttccct ggagtagtta ctttttctgc attgcttgat aacttgatcg tatccatcat
     2281 ttttccttca tcatcttcga tcgatactag cttgaatact gcaccaaggg ctggctgatc
     2341 ataggctgtg atcaattttg tcccaacacc ccatacatca attttagctt tttgcatctt
     2401 taaattcaaa atcgttgctt catccaagtc attcgaagca tagatttttg cttccgtaaa
     2461 tccggctgca tctaattgtt cccgaacacg cttagatata taggccatat ctccactgtc
     2521 gatgcgtacg ccttggaagt tgattttgtc acctaattct ttcgccacgc gaatcgcact
     2581 tggcacacct gatttcaacg tatcataagt gtcgactaag aacacgcagt ctttatgggt
     2641 tttagcgtag gccataaatg cttcataatc atttccataa gattggacta acgaatgagc
     2701 atgggtcccg ctagctggta tcccaaatat cttcccagca cgtacattgc ttgttgcatc
     2761 tgctccgccg atatatgccg ctctagttcc ccagactgct gcatccagtt cttgtgcccg
     2821 tctagttcca aattccaata gtggatcttc tccgatgaca gatttgatac gagctgcttt
     2881 tgtcgcgatc aacgtttgga aattgaccat attcaacagt gctgtttcta ctaattggca
     2941 gtgcgctaaa ggcccttcca cttggattaa tggttcattt gcaaaaacta gttctccttc
     3001 acgagcagaa cgaactgttg ctttaaattc aaaattttgt aaatactcca aaaaaccttc
     3061 aggatagact tctaatccac gcaagtaagc aatatcggaa tcattgaacg tcagattctc
     3121 taaataattg actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat
     3181 ttctcgaaag taacattcaa aaacagcatg taaatcagct cgacctaatt cccagtatgt
     3241 ctgcatcata ttgatttgat atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt
     3301 attattcatc ttgtattact cctcttggca agttttcatt tcctacccat tataacaaaa
     3361 aaaattaata ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt ttacggatat
     3421 ttcttgctaa aaatgaactc catctgctaa aactatcctt tcttattggt atttaaccta
     3481 aacgagaaag aatacttatt ttattcaatg tatccgagac acgaagaaat tagcgttttc
     3541 tatttttctt acttgataaa ataagtagat tttcctacta caatcgtagc cgttaagata
     3601 ccgatgattg aacaaatata accaaatgtc tctacaacat aatcaatggc aaaagttttt
     3661 gaagcaataa tacgtctttc cttttttgct tcttatcccc aaagaaacgg atataactta
     3721 ttatgtattg aatttttttg gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa
     3781 actcgcggaa aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa
     3841 tagaatggaa acaaaaatgg cgagacatca atcagtctcg ccttgttttt tattgtatac
     3901 attagatatg gagcaggtta ccataaagat gattattagt cgttattaaa tagaatcctt
     3961 cgtatttttt tgttctatca ctacaatatc tttatttgga cgtttgaatt gtattccggc
     4021 acgttctagc tcctgtttca ctcctacatg gactttgaca tcttctcgtg tcatttttaa
     4081 aacgtgatga atcgcatagt cgatttcttg ccttgtgatt tttttcttcg tattgatcat
     4141 cacgacttgc tttttatcat atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa
     4201 taatttgtaa taattaatag ccgtattccc aaaaaagaat tccactactt taggataaaa
     4261 tgtcttaata tttaaattac tattcacagg tgtttcgatt agcttcatct cattcctctt
     4321 ttctatatag tcgatttaga tccaatctta agttatgtta ccatctttcc tagcattaag
     4381 gaatcggtta caattgaacc tttaaagaca aaaggattta ccttttatca cttaatagta
     4441 taacataatt tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt acgtaaagga
     4501 ttatgcttac atttgtcagg aggaaaaata atggagtaca caataaagaa aatgtcagag
     4561 atatcaggag tcagtccgag aaccttgcgc ttttatgatg agattggact gctaaaacca
     4621 gctaggatca attcttcagg ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa
     4681 cacatcttat tttatcgaac aatggcattc aagttggatg atatccaaga ggtcttagac
     4741 aatccatctt ttgaccacca gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa
     4801 cgggcacaaa tcgataccct cttgacaact gtccaacaga cacttgatat gtacgaagga
     4861 ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta aacaacaaaa gttgaaagaa
     4921 aatgaagaat cctatggaca agagattcga caaaaatacg gaaaaaaaaa ggttacagca
     4981 tccaatgaaa aatggatgaa tatggataaa gaaacgtatg aagaaatgca aactgttgaa
     5041 aagcagatgt tatttgatct cactctttac ttaaaagaac cagaagatga aagcttagca
     5101 gaccgtatct tccaagctca taagaagtgg ttctcttaca gctggcctga atatcagcca
     5161 aaagcgcata aaggactagg aatgatgtac gaagccgatg aacggttcac tgcatattat
     5221 gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg
     5281 taatctttta catgatccaa caaaaaagta cacgtaaaat gacctaataa caaaacagca
     5341 gacacccaag ttagattttt atgctaactt ttggtgtctg ctccattctt tcacaagttt
     5401 tttcatccta ctttaaatat attcatcgtt gaataaacgt ataaggtatt ttcactttat
     5461 cctttttttc tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc agatggtggt
     5521 cgatggttgg gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg
     5581 gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa
     5641 agacaccatc aactccaagc tctttaaaaa agaagcctgc ttgttttcct ttctctgcat
     5701 ctatacaatc ccaaaaaaca agtgattcat caaaagatgg aaaaagttga tgacatagct
     5761 gcaaagtgat ttttgagtta taacttagcc tgccacttcg ccccaacgtg atgcccaatt
     5821 gctttatacc ttgctgcttc aaataagtaa gaccttctgt aattgattgt tctcgatcaa
     5881 taaagacaca gcttgcctgt tttcccttga tttcttcaca aaaaactaga ggtccatatt
     5941 tttgatatgc caataaatca tctagtgtat tggcacgaga cgtaatgatc aatccatcga
     6001 aagctttagc tgcaaattct tcaagatacc ttcgctcttt ttctttatga tagcccgtcg
     6061 gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc aatgatccca ctgatcaact
     6121 gatcaaaata cggatgattg gtataaggca gaatcactcc aatgcttttc gtttttccat
     6181 aacttaactg tctggcattt gcattaggga tataatcgac ttctttcatg accgcaagta
     6241 tttctttcct tttttcctca tctacatatg gatgtccatt gattacacgt gaaatagtag
     6301 aaactgaata acctgtgagc tttgcgatat ctcgaatgtt tgccactgct gcttcgccac
     6361 ctatacaaaa agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg
     6421 atacattttt ccttcatgcg aaaaactgat ccgctgcccg ccccaatata tatcttttgt
     6481 caaattttcg atttcattca ttggtatttc ttttactata ttcctattca acccttgaaa
     6541 actgtacaac gttaatacgg atcccttgaa ctcgacaaac gcataaaaat aattgttcgt
     6601 tgtgccatct gttatctcta cataagtatt cattgcaagc cctccttttg atactttgag
     6661 tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa aagttttatt
     6721 cctctttcat tattaccaaa gtctcttatt ttaaattgaa tacattataa tgatactgta
     6781 aatatatctt tattattagt gaat
//
LOCUS       DAJSNV010000120         6552 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSNV010000120 DAJSNV010000000
VERSION     DAJSNV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.124"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1273..1938)
                     /locus_tag="ON516_002530"
     CDS             complement(1273..1938)
                     /locus_tag="ON516_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345980.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ON516_002531"
     CDS             2118..4100
                     /locus_tag="ON516_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3345981.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ON516_002532"
     CDS             4104..4742
                     /locus_tag="ON516_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3345982.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ON516_002533"
     CDS             4770..5198
                     /locus_tag="ON516_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3345983.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6552
                     /locus_tag="ON516_002534"
     CDS             5655..>6552
                     /locus_tag="ON516_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3345984.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSST"
BASE COUNT         2244 a          996 c         1213 g         2099 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac ag
//
LOCUS       DAJSNV010000121         6457 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSNV010000121 DAJSNV010000000
VERSION     DAJSNV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.125"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(718..3867)
                     /locus_tag="ON516_002535"
     CDS             complement(718..3867)
                     /locus_tag="ON516_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3345985.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="ON516_002536"
     CDS             complement(3880..5079)
                     /locus_tag="ON516_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3345986.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..>6457)
                     /locus_tag="ON516_002537"
     CDS             complement(5069..>6457)
                     /locus_tag="ON516_002537"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3345987.1"
                     /translation="LLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLN
                     DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDG
                     DVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGS
                     GSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDW
                     PTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLK
                     ETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK
                     NRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKEN
                     DYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEMW
                     LQGALEVFKHEQ"
BASE COUNT         1789 a         1332 c         1066 g         2270 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaa
//
LOCUS       DAJSNV010000122         6311 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSNV010000122 DAJSNV010000000
VERSION     DAJSNV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.126"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(99..1229)
                     /locus_tag="ON516_002538"
     CDS             complement(99..1229)
                     /locus_tag="ON516_002538"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3345988.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ON516_002539"
     CDS             complement(1878..3080)
                     /locus_tag="ON516_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3345989.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ON516_002540"
     CDS             complement(3140..3343)
                     /locus_tag="ON516_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3345990.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="ON516_002541"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="ON516_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="ON516_002542"
     CDS             4880..5692
                     /locus_tag="ON516_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3345991.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1875 a         1297 c          996 g         2143 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt t
//
LOCUS       DAJSNV010000123         6285 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSNV010000123 DAJSNV010000000
VERSION     DAJSNV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.127"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            110..487
                     /locus_tag="ON516_002543"
     CDS             110..487
                     /locus_tag="ON516_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3345992.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            475..813
                     /locus_tag="ON516_002544"
     CDS             475..813
                     /locus_tag="ON516_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3345993.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            947..1390
                     /locus_tag="ON516_002545"
     CDS             947..1390
                     /locus_tag="ON516_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3345994.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1652..2467
                     /locus_tag="ON516_002546"
     CDS             1652..2467
                     /locus_tag="ON516_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3345995.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2477..3139
                     /locus_tag="ON516_002547"
     CDS             2477..3139
                     /locus_tag="ON516_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3345996.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3344..5008
                     /gene="alsS"
                     /locus_tag="ON516_002548"
     CDS             3344..5008
                     /gene="alsS"
                     /locus_tag="ON516_002548"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3345997.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5021..5731
                     /gene="budA"
                     /locus_tag="ON516_002549"
     CDS             5021..5731
                     /gene="budA"
                     /locus_tag="ON516_002549"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3345998.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5861..>6285
                     /locus_tag="ON516_002550"
     CDS             5861..>6285
                     /locus_tag="ON516_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3345999.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
BASE COUNT         2048 a          993 c         1350 g         1894 t
ORIGIN      
        1 ccagcttagt tttaatagaa atgagtattg acttggagta cacttaaagt tgtagaataa
       61 ataaaataca taagtaaagt ggcaagaaat taagagaaag agtgatcaga tgaatatcaa
      121 acaagtcagt gaagaaaagg gcatttcggc cgatacattg cgctattatg aacgaatcgg
      181 gttgattcca ccagttaatc ggacaaaagg cggaatccgt gattatacag aagaagacct
      241 aaaatgggtc gactttacga tctgtatgcg aaatgccgga ttatcgatcg agtctttgat
      301 tgaatatatt cgcctatcta gcgaaggaga agcaactgta tttgagcggc gtaagctttt
      361 gattgaagaa agtgaacttt tgacgaaaaa aattgctgaa atgcaagaat gtttggaacg
      421 tttacaagga aaaatcaaga aatatgatcg tgtgttatct agaaataaaa tggaatgtat
      481 caagtaaaaa aatcaaggag catcgctatg gagttacgct tactaaaata tttttggaca
      541 attgcagaag aaggaacaat ctctcaagcc gcagaagttc tacatcttac acaaccaacc
      601 ttaagccgac aactgaagga gttagaagag gaactaggaa cagaactgtt tgtacgcgaa
      661 aaccgcaaaa tgatcttgac agaagcaggt tattttctaa aaaagccgag cagaagaaat
      721 tttagattta acgaatcaaa ccactcaaga attcgagaac agaaaaaaac aattattcag
      781 cggccgattt acgatcggct gtgtggaagc tgataattcg aatacattgg cactattact
      841 agaaaagttg atccgtgatt atcccagcgt tcaatttcat atccacagcg gaacaagtga
      901 tgatatcacg gagtgactag aaaaaagcct gttagattta gcgattttgc tagatccagt
      961 agatacagag aaatacgaaa cgattttgtt accaagaaag gaaaaatggg gattgctcat
     1021 tttagaaaat tctgttttag caaaaaaaga cgtgataaca aaagaagact ttgcgaatgt
     1081 acctcttttg atctcaaagc gtccagaaat acagcgaatg tttgctgact ggttaggcat
     1141 aaaaaaggag cagttgctga tcacaggaac gtacaatttg attttcaatg tatttgcgct
     1201 aattgagaat caggtaggtg ctgctttagt catagaaggt gctgtaagtc atctttcaaa
     1261 gcgagtgaca ttcgttccgc ttgacccaat gattgaaacg aactgcatgc tagtctggaa
     1321 aaagaatcgg attttatcac ctgtagtcaa tgaattgatt caccgtttca aagaagcttt
     1381 tgaacagtaa taagtatctc taatcgaaga aatttcaata caaaagcgag acgtatgaca
     1441 gtcttgcttt tgtatttttt catttttaaa tcaaaaaaaa taaagagatt tacataactt
     1501 gttaaataag cgcttacaaa cataagaaga acggaaatga atcagctata ataaccgttg
     1561 ttctggattg taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga
     1621 aagaaaaaaa gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat
     1681 tctgctgatt gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc
     1741 attcaaagca aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca
     1801 tttagagaat tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac
     1861 agctttttta agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta
     1921 cgggttttct aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac
     1981 gattgctgtc ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg
     2041 tttaacatat acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt
     2101 cttgttgaag caatactaca gtagtatccc agattcttta tgtgagagtg cgcggatcga
     2161 tggcttatca gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat
     2221 tgccagtttg acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat
     2281 ttatttgtct tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct
     2341 atttgatgca gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc
     2401 ttttatattt ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa
     2461 ggaataggag gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt
     2521 ttattggttg tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta
     2581 tatagcagct gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc
     2641 tttacttttt attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa
     2701 agaaaaaagt ttttcaccag tacgtttgtt cataaagacg tttcgccaat tttggctaaa
     2761 gggatttttt tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt
     2821 cttttttata cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg
     2881 tttatcagtt tcctttagca tcaactactg gtattttcag gtgagaaatc cagcgtcgaa
     2941 accaaatcaa gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt
     3001 actagatttt ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg
     3061 tattctacta ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat
     3121 gagaactatg gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt
     3181 atgtaaaaac tgtcattctt tttcattttt tgaaacatat ggctaaacaa atgaaataaa
     3241 acgtgctata cttttgaatg aaacaggttt agaaactgta tcaattattt gtgattattt
     3301 ctaactaata attaaaaata aagcataaaa aggacgatgt tcaatggacg aaaaaatgaa
     3361 acaaggttcg aaatctgtag tagaaagttt gattaatcat catgttgatt atgtattcgg
     3421 tataccaggt gcaaaaatcg atggtgtatt taatgaatta gaggatcaag gtcctgaatt
     3481 aatcgtgact cgtcacgaac aaaatgctgc atttatggca caagcaatcg gacgtattac
     3541 aggagagcca ggagtagtta tcgcaacaag tggtcctggt gctagtaact tagctacagg
     3601 tttggtaaca gcgacagcag aaggtgaccc agttttagca attgccggac aagtaaaacg
     3661 aagcgatcta ttgaagttga cccatcaaag catggataat gcagcactgt tccagccaat
     3721 cacaaaatac agtgcagaaa tccaagatcc agaaacaatt tcagaagtga ttgccaatgc
     3781 ttatcgtatg gctaagagtt caaaaaaagg cgcgagcttt atcagtatcc cgcaagatgt
     3841 agtggatgca cctgtccaag ggaatgtcat caagccattg agcgatccta aattaggttc
     3901 tgcttctgca gatgatatcc gttacctagc tgaacgcatt agagaagcaa aactaccggt
     3961 actattagta ggtatgcgag gatcaagtga aaaagaaacg ttagccatca ggcaattagt
     4021 tggaaagaca gcattgcctg ttgtagagac attccaagca gctggagtga tctcacgtga
     4081 attagaagcg catttctttg gtcgtgtggg tttatttaga aaccaaccag gagatatgct
     4141 tttgaaacga agtgatctag ttattgctat tggctatgat ccaatcgaat atgaagcaag
     4201 aaactggaat gcggaaaaag atgcgcggat cattgtgatc gatgaagcgc cagctgaaat
     4261 cgatcctttt atgcagcctg aaagagaatt gatcggtgat atctcagcca ctcttgattt
     4321 attgacaggt tcattagagc ctcaacaagt gtcagaagat gcaaaagaat atttagcatc
     4381 tcttcaagca gaattaacgg agcgggacat tgttcaatca aaaggagaag caggtatctt
     4441 gcatccttta gaagttatta acacgcttca gtcaaaagta acagatgata tgactgttac
     4501 cgtagatgta ggtagtcatt atatttggat ggcacgccac tttagaagtt atgaacctcg
     4561 tcatttgtta ttcagtaatg gtatgcaaac gcttggtgtt gcattgccat gggcgatttc
     4621 tgctgcattg gtgcgtccaa atacacaaat cgtatcagtt tccggcgatg ggggattttt
     4681 attctctgcc caggatttag aaacggcggt acgtaaaaaa ttgaatatcg ttcatttgat
     4741 ttggaatgat ggacactaca atatggtaga gttccaagaa aaaatgaaat atcagcgggc
     4801 gtcaggtgta gactttggtc cagttgattt tgtgaaatat gcagaagctt ttggcgctaa
     4861 aggaatccga gctacaagtg tagaggaatt agaaaaagct ttagaagaag gatttgcgac
     4921 agaaggaccc gtaattattg atattccgat tgattaccgt gataatgaga aattgggaga
     4981 aacaatctta ccagatcaat tctattaagg aagtgtcgac atgactgaaa aaatcttata
     5041 ccagcacggg acattaggag ctttgatggc tggcttgatg gatggaacag aaacgattgc
     5101 tcatatatta gaaaaaggaa ctctgggatt gggaacattg catgggcttg atggtgaagt
     5161 catcttttta gatggcgtag cttatcaagg aagatcagat ggctcagttg cccaattaga
     5221 tggaagtgaa cttaccccct atgcagcaat tacagatttt acgccagata ctagtttttc
     5281 tgtctcaaaa actgcagatg gagaacaatt gaaaaaagac atattagtaa gagaagccgg
     5341 ggaaaaccta tttttggcag tgaagataac tggattattc aaaaatatgc acatccgtat
     5401 catgccgaag caagaaaaac cttacagaag actagcaaaa atttcagagt ctcaaccaga
     5461 attccaacaa agcaatgtcc aaggaacatt agtcgggttc tttacgcctg aattattcca
     5521 aggagttgct gcagcgggat ttcacttgca ttttatcgat gatacacata catttggcgg
     5581 acatgtaatg gattttgaag tggctgaagg aaaagttgag attagccgca tctcgtccct
     5641 tgtacagcat tttccagttg atgatccgac ttttgttcac gaaacgattg attatgctga
     5701 tttagcagca gaaatccaac aagcagaata gaaaaagaca gcgagatgcg ttcaaggtga
     5761 ttaacctgac gtatctcgtt tttttgagga ggaaacctag cgtttacaaa aaaactgagc
     5821 tataatcttc tccatagatc aaaaaggagc ggtttttatc atgtggacag aaaaacagaa
     5881 aaaagatgca tacgaaattt tattattaca gcaacaagga ttattagaag cagaagataa
     5941 ctggttagca aatttagcaa attcatctgc attgttgaat gagactttgc ctgagactgt
     6001 ctttgcagga tattatttat tcgatggtaa tgaattgatt ttaggaccgt tccaagggcg
     6061 agtttcttgc acacgtatca agatgggaaa aggagtttgt ggagaatcag ctgaaaaaca
     6121 agaaacattg atcgttgatg atgtcaaaac ccatgcaaat tacatttcct gcgattcagc
     6181 cgcacaatca gaaatcgtag tgccaatggt aaaagaaggt aatttaatcg gtgttttaga
     6241 tatcgattgc ggagtaacga aagggtatga tgaaatcgat caagc
//
LOCUS       DAJSNV010000124         6033 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSNV010000124 DAJSNV010000000
VERSION     DAJSNV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.128"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..3135)
                     /locus_tag="ON516_002551"
     CDS             complement(<1..3135)
                     /locus_tag="ON516_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3346000.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLE"
     gene            complement(3149..4366)
                     /locus_tag="ON516_002552"
     CDS             complement(3149..4366)
                     /locus_tag="ON516_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3346001.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4356..5951)
                     /locus_tag="ON516_002553"
     CDS             complement(4356..5951)
                     /locus_tag="ON516_002553"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3346002.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1654 a         1282 c         1004 g         2093 t
ORIGIN      
        1 ttcaagtgga atcaactcat catctaacat tttacgccat gctcgcttca tttcttgtgg
       61 gtatttaaat ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt
      121 gataattgct ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc
      181 atgtaattct ttctcagaaa caaaccactc attggaaaag gcatgaatca aattatcact
      241 tgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat ctaaagtccc
      301 cacatcaaat gcatttaaaa tttgtcggta aacttctttg actccttctg ttttattcaa
      361 tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgcag cataagttcc
      421 tagtaattga tcaatataag tagaatcaat atcatacaac ttagtggtat tttcactata
      481 aaattcgatc gttgaaaaat cttctccctc tttttctggt tcttcctcca tcaatgaacc
      541 cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat tgttcaaagt
      601 tgaagattgc accatgtcat cattgtaatc atcataagtc actaacgatt catacgcacg
      661 attcaattct tgaaatgctt tgacgtactc gatacggatt tcaagtggcg tatgttcatc
      721 aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga atcttttttt
      781 ggattcttgg taacttggat aaatcaaacc cgattcttct ttagcttctg aatacagttt
      841 tgtcgcatcc tcgacattct tctccattgt cgctggcttt cggaaagtaa caattagtcc
      901 ttttgttttt tcaggatacg tacggttcgt acgtgaaaag gcttggatca atcctgcata
      961 ctctagatta cgatccacaa atagggtttg aatcgtaggg gcgtcaaaac ctgtcaatag
     1021 acgatccact acgattacta aatcgatttg tttcccaaat tgtttgaatt ccgctctttt
     1081 tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta gtgaccaggc
     1141 agtatcataa tactgattat attcctcaat aattttttgc atttcttctt gttgggcact
     1201 tgaatctcgg ctattttctt ctaatgaata ggtaatagcg attcgtggga aatcaggatc
     1261 ctccatcgtt cgtccttcac ggataggctg attgggaaac tcttttatta accaatcaga
     1321 ttctttcgtc atctctttga tggcccgata atactttttg gccatatcaa tggaactcgt
     1381 ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg tataggcatt
     1441 gtctggacgg aacattttat gaatgacttt ttggatatgt tcctctgctt caaaaacagt
     1501 agggtccaaa taactttctt ttttgacaga ctccatttga tcgatcatac gattgatctg
     1561 ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga ttttattctc
     1621 taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa ccgagccatc
     1681 ttctaatgca ttcttgatgg tataggtgtg caagacctct ccatactgat catgggtcgt
     1741 acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg tacctgtaaa
     1801 gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt cagcacttaa
     1861 cgctcgatga cactcatcaa ctacaaacac gatatgttgc cccattaatt tttggaagcg
     1921 attggttcct tttttctctt cctgtttctt tgcatatttc aacgccgcat ctagtttttg
     1981 acgggttgta atgatcacaa cattcgcatt ggcatctgcc aacagagttt cacttaactc
     2041 tttggcactt cctgttccca cgatcaagct attcgctttg gcattaccag aagaaacccc
     2101 agtattaaac tccgaagcaa atttcgtaaa ctctgtggtg gtctgattat ctaaatcttt
     2161 gcggtccact aacatgatcg ttcgatcgat ccccgatttt cgtgctaaaa gcttagtaga
     2221 tacaaaacta gtcaacgtct ttcctgaacc agtcgcatgc cacacatacc ctgattgatg
     2281 tttatttgcg gcaataaaca aggcttgaat cgcatgcact tgataaggat gtaacaccat
     2341 gagggtttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca agcgatgggc
     2401 atctgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct ttttattatc
     2461 tttcgttcgc caactaaata aaaacttagg atgcaaatcc ttcggtaagg catttgcaaa
     2521 ataccgagtc gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa agatattgtt
     2581 ccgaaacatg ccttcttcag catatttttt gatttgatta aacgcctgat aaaaaccatc
     2641 ttttgcagtt acttgtttta gctcgatttg gataatcggt aagccattga tcaataaggt
     2701 gacgtcaaaa cgtcgatccc gcgcttctat gttacttcca cgttttgcaa tttgatggac
     2761 cacttcataa gtagagattc ctccaccaat gtcttgattg gaataaagga taagtgagac
     2821 agaacccaat tgcggatttt cccgttcaat cgtgatccga gccatcccat tttcaccttt
     2881 taaccatttc gcagcatcaa atggggtttt cgtccgtagc aataattcgg ttttgatcgt
     2941 ttcaaactct ttatcggtca aaggagaatc attcagttct gcttgattat ttgagatgat
     3001 tttttgacgt aaattgctcc aaagatcttc ttctgatttt agatccggac gatacgtcca
     3061 ctgattatgt ccttccccga ggacacgaat caactgttct tcgacttccg cttcctcacg
     3121 gtgactaatt ttcattcttc ttccctccct aaacaaacat cttttgcaag aagccttttt
     3181 tcgtttcctt cagcaaatct aacttacgtt gatgaagagc gatcgtatca tctagctgct
     3241 tgaagaaggc accaattttt tgttgttcat ttatatccgg tggaaactga aatgtccttt
     3301 gttctatacc acctttagtc acatgtacca ttgtcgttcc gttagttgat tctttaattt
     3361 tctctttatc agtcaatagc caagtatata gatactgttt gtccaattcc ttagataaaa
     3421 atttaagttt ccaaatatga taatggtaga ttacacgttc ttcattccac atttcaggac
     3481 cgaagcttgt tgcccataaa tataagagat ctcctttatt agcatacttg tcttcctcta
     3541 attctaagtt tgaataatac catctatcat tagtattaaa attacctact cttaaaaccc
     3601 tatattttcc agaatcaagt aattcttgtt gtttaaaagc tctaccattc aaaaaaacta
     3661 ccacttcccc caacttccgc tcttcccaat ctcccgtaaa tcctggaaaa cgaacttctg
     3721 gcacttttgc tccgttttta gggaacattt tttgtaagaa tccttttttc gtttccttca
     3781 gcaaatctaa ttcgtgctgg tgaagcatga tggtgtgatc aagttgtttg aaaaatgaac
     3841 cgatcttgat ctgttcgtca taagttggaa gtgagatagg tgtcttttta ccactatcaa
     3901 ttgtataggt tccgacggtt ccaatgcctg caacgtcacg gatatacttc tgaattttgt
     3961 tatttatgaa cgagtagtaa aagaagtttc cgtcaatttt atgctcattc ttgaaccaaa
     4021 tgatgtttcc atccttaaag taaattgggt tatcatcagt gattaataag gggacaccta
     4081 ttgatccgac accagttaca agcaagtctc cttgagaaac tttacctgaa tttttcgaat
     4141 attcattata tttttcttct gaaatgtaaa gataatcgga tggttcctca ttttttgcag
     4201 ctgagacaat atcacgagca cgtaaaaatc taatacctga gttagtccaa tctgattgat
     4261 gaattcgctt cacagaagta acatccatca tatctcctaa cttacgctct tcccaatctt
     4321 ccgtaaaacc aggaaaccgg atttctggtc gtgtatcatt gctcatgttt gaacacctcc
     4381 agcgcacctt gtaaccacgc ttcattttca ggtgttgttt gcagggaaga gatagcttct
     4441 aaaagttctt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc atttccgaca
     4501 tgaaccatat caattggttc ttcttcttcg aaagtatcga catagcgtgg gatatttaag
     4561 ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt ctcgacatct
     4621 tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgataa tttattctga
     4681 tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg agtttgacga
     4741 ttcttcttca atacaatcac cgtcgttgga atcgatgtac caaagaacaa gttcgcaggc
     4801 ataccgatca cagcataaat actgccgtct tctaataatt tttgacgaat gaccccttct
     4861 gcagcaccac ggaataaaac cccgtgtggc aagacgatag ccatagtccc tgtttccttc
     4921 aaatgataga aaccatgaag aagaaacgca aaatctgctt tggattttgg cgctaatttc
     4981 ccgtagcgat taaaccgaga atcatctaag aaggtcgtat ctgctgacca attagccgaa
     5041 taaggtggat tcatcactac tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta
     5101 ttcaacgtat ccccattgcg gagattcatt tcttcggcat ctaccccatg taaaatcaag
     5161 ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata tttaacgtta
     5221 tctggatgag ttaagtagtt acgtacattt aacatcaagg aacctgaacc catggttggg
     5281 tcaaagacac taaagaatcg tcgttctttt tgatccaacg tcacgatttg tgccatcata
     5341 tcagaaacca tatgaggcgt gtaaaattct ccggctttct tccctgcttc tgaagcaaat
     5401 tgactgatca gaaattcata cgcatccccg atcacatccc catcgtgacc taaaacttcc
     5461 acgtcattca atttcttgat aacttcggta atcgtcacat ttcgttgttg ctcatctgtt
     5521 cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg attgtaggta
     5581 gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct
     5641 tgatccgcta agacgttgaa aagatactct ggtgagatcg aataccccaa aatatcaacg
     5701 attgtggcaa tcaggtcttc ttttgattct tcatctgaaa gtaattcacg atacaacatt
     5761 gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt
     5821 tccaacaaac ggtcggataa atatttatag aaaatcagtc ccaataaata atttttatat
     5881 tctgacgcat ccattttact acggagatta tctgccgcac taaataattt ttgatttaat
     5941 tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt
     6001 ttagcatacc cccttttata aaaagcaaaa aaa
//
LOCUS       DAJSNV010000125         5901 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSNV010000125 DAJSNV010000000
VERSION     DAJSNV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.129"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(376..1083)
                     /locus_tag="ON516_002554"
     CDS             complement(376..1083)
                     /locus_tag="ON516_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346003.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1148..1984)
                     /locus_tag="ON516_002555"
     CDS             complement(1148..1984)
                     /locus_tag="ON516_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346004.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2002..3411)
                     /locus_tag="ON516_002556"
     CDS             complement(2002..3411)
                     /locus_tag="ON516_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3346005.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3425..5248)
                     /locus_tag="ON516_002557"
     CDS             complement(3425..5248)
                     /locus_tag="ON516_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346006.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1826 a         1277 c          953 g         1845 t
ORIGIN      
        1 tacatatgaa acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata
       61 aaatatacaa atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac
      121 acttatatat caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata
      181 gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg
      241 tataagtacg gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca
      301 ttattcaata acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac
      361 cacagaataa tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg
      421 ctctgtcact gcattactga tacctgttag attagctaat aaatctgata aagattgagc
      481 atctgtagaa atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt
      541 ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt
      601 cttcccagta tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt
      661 ctgctccgta actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact
      721 tatatattgt ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc
      781 actataacca gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga
      841 aaaatagaca tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac
      901 ttgagacctt cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt
      961 attattaatc tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca
     1021 ctttaccact tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc
     1081 caaggaatca ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt
     1141 tcttcattca gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg
     1201 ctgaggcacc catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt
     1261 tcggaaaacg ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct
     1321 tcttaatcat tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg
     1381 aatctttatc aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta
     1441 gtgctcttac cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa
     1501 tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga
     1561 tcaattggat agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa
     1621 tcagcaggcg attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt
     1681 gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat
     1741 aatcgataat tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa
     1801 tgtaatcctt ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt
     1861 cagccggaac caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta
     1921 cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc
     1981 tcattagatt caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca
     2041 aaagaagctt tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac
     2101 atatataaaa ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga
     2161 ccaaggtaaa taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat
     2221 atgtttggct agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat
     2281 gtcgggtgcc ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa
     2341 tttcctcaat gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc
     2401 tacataagga ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc
     2461 ccccgctcta gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga
     2521 ataataatta atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc
     2581 ctcaatttta ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt
     2641 tccccgaact gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc
     2701 aattgcatct tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc
     2761 gatttttcct ttaagattca actgatgata caagtgaatt actctagctt gagctaataa
     2821 aacatggtga ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa
     2881 accaatcgct ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt
     2941 caccttgtca ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt
     3001 tgctcgattt aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat
     3061 ggtgacaatc ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga
     3121 aattcccttt tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga
     3181 aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg
     3241 gtaaaaatcc gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat
     3301 aatagacggt tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga
     3361 ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct
     3421 ctccttaact aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa
     3481 ttcctgcgta attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt
     3541 ttctaatggc agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg
     3601 cttttacaaa tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga
     3661 tcaataattc aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag
     3721 taatgacttc tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc
     3781 cctttcccaa tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg
     3841 ttccttttac cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat
     3901 cagaagtatc gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta
     3961 cacacgtaat aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg
     4021 ctgctaagcc aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac
     4081 cagcaattgc accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc
     4141 catagagtga aggctcagta attcccatta aagcagtgat cccagtggac acagctagct
     4201 gcttcaactc tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa
     4261 tattagaagc cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca
     4321 tagtagcgat tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac
     4381 cacccatcaa aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg
     4441 ctataagatc gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca
     4501 taattaaaaa taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat
     4561 ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc
     4621 ccgcataatc tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa
     4681 aaagctgtga aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt
     4741 tgacatcaaa ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg
     4801 catcagcaat agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca
     4861 aagtcaagac ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg
     4921 gtgtaaaggt ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg
     4981 aagaggggcc ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt
     5041 ggacttcatt gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac
     5101 caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca
     5161 gtctggtggc acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata
     5221 catttcttcc tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc
     5281 aagtcaaacc acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata
     5341 atcagtaaca tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc
     5401 gcaatcaaca gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca
     5461 gttagatcac atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta
     5521 atattaacta taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg
     5581 aaagcatcaa aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa
     5641 attgatgaat ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa
     5701 tagtgatact tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga
     5761 gatataaagg tatcaataga atgatttggg ctagtattct taaaaattaa ctagcaaatg
     5821 tttaaactga tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa
     5881 taaaaatacc aaacatatcc a
//
LOCUS       DAJSNV010000126         5786 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSNV010000126 DAJSNV010000000
VERSION     DAJSNV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.130"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(440..943)
                     /locus_tag="ON516_002558"
     CDS             complement(440..943)
                     /locus_tag="ON516_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3346007.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1302..2327
                     /locus_tag="ON516_002559"
     CDS             1302..2327
                     /locus_tag="ON516_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3346008.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2650..3528
                     /locus_tag="ON516_002560"
     CDS             2650..3528
                     /locus_tag="ON516_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3346009.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4004..4120
                     /locus_tag="ON516_002561"
     CDS             4004..4120
                     /locus_tag="ON516_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3346010.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5048..5386
                     /locus_tag="ON516_002562"
     CDS             5048..5386
                     /locus_tag="ON516_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346011.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5480..5710
                     /locus_tag="ON516_002563"
     CDS             5480..5710
                     /locus_tag="ON516_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346012.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1964 a          868 c         1069 g         1885 t
ORIGIN      
        1 acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga
       61 atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat
      121 aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata
      181 aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta
      241 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
      301 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      361 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      421 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      481 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      541 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      601 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      661 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      721 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      781 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      841 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      901 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
      961 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
     1021 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
     1081 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
     1141 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     1201 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     1261 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     1321 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     1381 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     1441 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     1501 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     1561 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     1621 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     1681 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     1741 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     1801 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     1861 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     1921 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
     1981 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
     2041 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
     2101 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
     2161 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
     2221 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
     2281 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
     2341 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
     2401 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
     2461 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
     2521 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
     2581 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
     2641 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
     2701 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
     2761 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
     2821 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
     2881 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
     2941 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
     3001 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
     3061 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
     3121 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
     3181 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
     3241 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
     3301 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
     3361 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
     3421 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
     3481 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
     3541 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
     3601 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     3661 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     3721 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
     3781 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
     3841 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
     3901 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
     3961 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
     4021 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
     4081 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
     4141 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
     4201 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
     4261 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
     4321 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
     4381 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
     4441 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
     4501 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
     4561 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
     4621 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
     4681 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
     4741 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
     4801 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
     4861 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
     4921 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
     4981 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
     5041 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
     5101 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
     5161 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
     5221 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
     5281 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
     5341 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
     5401 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
     5461 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
     5521 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
     5581 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
     5641 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
     5701 gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt
     5761 cgatatcttt gctgttaata ttacta
//
LOCUS       DAJSNV010000127         5431 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSNV010000127 DAJSNV010000000
VERSION     DAJSNV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.131"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(12..482)
                     /locus_tag="ON516_002564"
     CDS             complement(12..482)
                     /locus_tag="ON516_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3346013.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ON516_002565"
     CDS             complement(641..1099)
                     /locus_tag="ON516_002565"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3346014.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ON516_002566"
     CDS             complement(1270..2415)
                     /locus_tag="ON516_002566"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3346015.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ON516_002567"
     CDS             complement(2428..3345)
                     /locus_tag="ON516_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3346016.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ON516_002568"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ON516_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3346017.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4476..>5431)
                     /locus_tag="ON516_002569"
     CDS             complement(4476..>5431)
                     /locus_tag="ON516_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3346018.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1636 a         1010 c         1031 g         1754 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc
     4441 ctgtgtaaaa tactatacaa tgctctcact attttttact tggcaaattt aatatatttt
     4501 cttgatgaag aaagccactc atcgtgttga tcaataagga ctgctccgat taaacgattg
     4561 gctgaaacgc agtttggaaa gatacgaata attttttctc ttctgcgcac ttcttgattc
     4621 agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg tacggtatat
     4681 tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata ttttggttga
     4741 tcgatataat catgaatcaa tcgattttta gcctcacgag ctaagttaat atctgtgaac
     4801 ttaaaaattc ctttaacagc ttctctgaaa gattttgaat tttttttagg aatggtggta
     4861 aagatatttc ttaggaagtg aacttggcat ctttgccaac ttgcgttggt gaaggatttt
     4921 ctaatggcag agactaatcc tttgtgcgca tcagaaataa cgagttctgt accttgtaaa
     4981 ccgcgttctt ttaggtattc aaaaaatgtt atccaggtct cttcgctttc gccactttga
     5041 atcatgaagc cgataatttc acggtcgcca tctttggtta ttccaatcgc tatatgacag
     5101 ctttttgaga gtactcgatt ttcttctcgt acttttatat agagtacatc ggtcattaag
     5161 taaggataat ttttttctga taataaacga ttctgccact cgttaaccat tggttctagc
     5221 tgttctgtta agctagaaac gaaggactta gagacggatt taccacaaag ttcttccaca
     5281 atttttgata ctttacgagt tgaaacgcct gatacataca tttccaacat tgaagcaatg
     5341 agggcttttt cgtttcgttg ataacgttca aacactgtgg gtgaaaaatg gccatcacgt
     5401 gttctgggta cttttaattc tagcgtgcct a
//
LOCUS       DAJSNV010000128         5348 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSNV010000128 DAJSNV010000000
VERSION     DAJSNV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.132"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            28..2355
                     /locus_tag="ON516_002570"
     CDS             28..2355
                     /locus_tag="ON516_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3346019.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2481..2810
                     /locus_tag="ON516_002571"
     CDS             2481..2810
                     /locus_tag="ON516_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3346020.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2850..3854
                     /locus_tag="ON516_002572"
     CDS             2850..3854
                     /locus_tag="ON516_002572"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3346021.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3851..5280
                     /locus_tag="ON516_002573"
                     /pseudo
     CDS             3851..5280
                     /locus_tag="ON516_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1894 a         1027 c         1059 g         1368 t
ORIGIN      
        1 tttgatgatt ttttattgag aggagggatg aaaatgatta agaaaactca aaataaaatc
       61 caacgtatat gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa aagaaaatac
      121 ttacatccca ttatgatttc gatcatcttg atttttactt tcttagggag cttatcaagt
      181 atttatgtaa acgtttatac agaaacgtca aattttgcta accaaacgac agaggagaca
      241 aatagtccac cctattctga aatacagtcc atagaaagca ccaatcagtc tagcaacaac
      301 gagacaacct caaccacatc ctcgactcaa ctgattgatc cgacagaaaa agaaatcaca
      361 gcagcaaaag cagcagggga gaaagcttat tcagaaacag gtagaagcca gacatttagt
      421 actgttgcgg ccactgcgaa tttcaacgga cataccatac cagatggata tacagtgata
      481 tatgccaaag atggagtcat tgttgcggtt aacggatcga tgagtgcgga taaaactgcg
      541 ttacaaggcg taaccaaagc aatcgctttt attgataacc gtccagatat ccataatctg
      601 tttattggct ccacagtaaa tggtactact atcacattcc caaaaagtat ctatatgggg
      661 gtggctaccg ctggaaatac gatcgattca gttacaccca atgcggataa aagttattct
      721 tttacaccct caacatgggg agggcttcct gccggaggag gtatgagtac tttttcaatc
      781 ggtatacagt tagcaaatga tattccattc acggggtact cgatcaaaaa tgggcagatc
      841 gttgatggta agggaaatgt ttcaaatttt ccaacctatc aagcagcaac ggttcaggtc
      901 aatgcgaatc ggacaggaac tttttcttat acagtcaatg gtggagcagc aaaaatcgga
      961 acgtacggga ctacattatc tggaatccat gtgggtgaca aagtggttat cactccaaca
     1021 gcagtaacgg gctattcttt tgcacaaaca aacggttctt tcacagccct agaaagtaac
     1081 gtcgatacgg tgacctatac aggcaaacaa acaccaatca ctattaagta tcaagactat
     1141 tttggacaga tgattgctcc ttctaaaata atgaatctta cttacggaag tgctgcacaa
     1201 gacctgacga ctaatacgcc tactatcaac ggatatacct ttacagctgt ttcagcgaca
     1261 gaaacaaaga accaatcagc aatgagtgtt ttagctagtt tagatacaaa tggaaatgta
     1321 atcgtaaagg acgcaaatgg aaaacagatt tctgaagtca tcctatatta taagacaaat
     1381 gcaacagtat caatcaatgc aaatggcagt aagtactatg atggtctttc tgttgtaccg
     1441 atcgttaaat atgcctttaa tgataaaaca gaatcaacat cccttttaaa caatgatctc
     1501 agtattgatc aaatcacatg gaatccaact gatttcaaag cgatgaatga aaagggagtt
     1561 gaagtaactg cgcctactga aattggaacc tatacgtctt ggcaattgac aaaaagcgga
     1621 ctagctaaac tagctgccag aacaaactat ttatttacta tcgtgcagac atctgatgtc
     1681 tatacgatta agcaaatcag cggtgcggta gagctcagtg gcagtaagac atatgatgga
     1741 aaagcaggtg tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag ttcgcttact
     1801 ccagtacaag tggcacttag cagtatggat tacacagcag atgcacaatc agcaaaaaat
     1861 atggttaatg ctggcagcta tacaattaaa ctaacgaata gcggaataga caaagtcaaa
     1921 gcagcaaatc ccaagttgtc atttactgat atagccaaca catcaggaac ttataccatc
     1981 tataaagcta acgcagtgat tactgtcgat gacgctgcct ttaattatga tgcccaaagt
     2041 cactcgattt cggttggaaa tctccacgtt accggtgttg tcttaggcga atcattggat
     2101 tataccctaa cagttaacag ccggacagat attggatcac aaatagtaac gattagttta
     2161 ggcgaaggaa gtgtaaacaa taattatacg attatcgcaa cagataccgc taagttgacg
     2221 atcaatccga ctctcttgct tccttcgaca ggtgggatag gtctactgcc ctttgtcttt
     2281 ttaggactga tctttatttt ttctggcttc ttttatttta tccatcgtaa gaaaaaagca
     2341 ggtgaacagc gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct gttctctttt
     2401 ttatcctact ggtctttttt ataggttctc cgactgtgtc agctagagaa tcacctatta
     2461 catttacttt gcataatcga atgttcgaaa gcgataagat gccaagtatc agtcaaaacg
     2521 acggaataaa tatcccggat aactttcaag gagtaaaagg gttgaacggc gttactttcg
     2581 ctatatatga tgtctcggat gaattttaca agctgcggtc agaaggaagt agtgtggaag
     2641 acgcacaaag aaaactagca caaaaaagtg atagtgaaaa aatactggca gaaaatgtga
     2701 caaaaacagt agacgaggaa gagggcattg cttcttttag tgcatcggac aaagatgagc
     2761 aaggtcgtga cgctgtttac cgatttgcgg aaataaagac ttcagattaa gtaaaagaaa
     2821 aatcagcacc atttgtcgtt gtgcttcctg tgacagattc atctaatcat aaattgacga
     2881 ccatccactt gtatccaaag agcgaacaaa aaatacctgc tgcactcaca ttaacaaaaa
     2941 cagtagagaa taagcagaca gattttgcag atggggataa agttccttat ctcatcaccg
     3001 taacaattcc tgagaacatc aacgagatag gaacctatac gatcaaagat acagctgatc
     3061 ctcagttatg gctagaacca gaaacgatcg attttttgat tggtggagac aagattcata
     3121 catttcacac acaaaaaaca aaacatggtt ttgtcttatc agattctgga aaagatctta
     3181 gttcgtttgc tgggaaaaaa ttaacgatcc gttaccaaat gacattgaaa gaaaacagtg
     3241 aaaaaacgac atttgataac gatgtacgtt tgacgactga caatcaaaca gtagaaacac
     3301 atcttgccat ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa acgaaacaaa
     3361 aactagcaga tgctcaattt ctagtcaaaa aaaatgacca atacttagcg caagaaaacg
     3421 ggataaatca ttgggtagcc aaagatgatt caagagcaac gatttttact tccagtaaag
     3481 atggaacatt ttctgtccat ggattgtctt ttggaagtta cgaactagtg gagattaaac
     3541 cgcctaaagg atatatattg agtcaatcaa ccattctctt tgaagttgaa aaaggcagtt
     3601 atgatccgct tcatcctata ccattggaaa tcatcaacga accaaaagca aatccgacga
     3661 ataatggaaa aacgccagtt cagatgcaga caaatggtcc atcaaatgaa aaaaatggag
     3721 gaagctttcc aaaaacaagc gaagagatgc ttggaggttt ctcaatttta gggttgttgc
     3781 tagtcctttc aacagggaca gcttggtttt ataaaaaaca acaaaaagga aacaatagga
     3841 gggaaataaa atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa ctgctatcat
     3901 gctgcttcct ttcgttctcg gcctaggaac tgcagaggtt tctaggccgt atcacctaca
     3961 ccagaaaatg ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa
     4021 ataaacaacg gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc
     4081 aacgtatatg acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac
     4141 gctacaccct tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca
     4201 ggtaaatctg atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc
     4261 gaaacgtcgc caaatatcgt gttaagctta cccttttata acccgaattc atcagaccct
     4321 gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga aagcaatatc
     4381 aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc gcaattccag
     4441 atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg tgtcgctctc
     4501 tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc catcagtgtc
     4561 aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga tggttatcaa
     4621 ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt gcaacaaaca
     4681 accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg tgtagtcaat
     4741 cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa ctacggtgat
     4801 ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc agaattttct
     4861 attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa tcttgcctca
     4921 ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga agaagtgaaa
     4981 ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg ggcatactgc
     5041 cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc tgcgtacact
     5101 gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga taaccaacct
     5161 aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt ggtaggtact
     5221 gctttagttg gaggagcagt gatctacttc aaaaaacgcc atcaagaaac agaagcttaa
     5281 actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg gggagaaagg
     5341 ttgtgaca
//
LOCUS       DAJSNV010000129         5219 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSNV010000129 DAJSNV010000000
VERSION     DAJSNV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.133"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            31..103
                     /locus_tag="ON516_002574"
     tRNA            31..103
                     /locus_tag="ON516_002574"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Lys,seq:ctt)
     gene            265..486
                     /locus_tag="ON516_002575"
     CDS             265..486
                     /locus_tag="ON516_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346022.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(810..1055)
                     /locus_tag="ON516_002576"
     CDS             complement(810..1055)
                     /locus_tag="ON516_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346023.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1049..1450)
                     /locus_tag="ON516_002577"
     CDS             complement(1049..1450)
                     /locus_tag="ON516_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3346024.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1622..3430)
                     /gene="uidA"
                     /locus_tag="ON516_002578"
     CDS             complement(1622..3430)
                     /gene="uidA"
                     /locus_tag="ON516_002578"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR3346025.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3458..5218)
                     /locus_tag="ON516_002579"
     CDS             complement(3458..5218)
                     /locus_tag="ON516_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR3346026.1"
                     /translation="MKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSR
                     DKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTD
                     GTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRS
                     FGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNS
                     FGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLG
                     FNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGII
                     SEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAIT
                     LVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVE
                     KYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWP
                     ASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGL
                     SEFKGISPIDAYCGLKDTFI"
BASE COUNT         1631 a          995 c          699 g         1894 t
ORIGIN      
        1 ttaggtgtta gaataacaaa gtattaattt gggctgttag ctcagttggt agagcagatg
       61 actcttaatc atcgggtcgc gggttcgagc ccctcacagc ccattgggtg ccaaaccctt
      121 aaatacagtg taaacgttgg tgcttagcat ttagaaaacg tatatactgt atttttttgt
      181 tttcaaaaga gactgtagta cccctttttt tcactaaaag cccactgtag tcattgatat
      241 ttttgataag aaaggaacat aattatgcgt aatgaaattg ctaatataac aataatccga
      301 gaaactttac aaaaccatac tgcaaatgag atctctaaac atacaggatt aaatttgagt
      361 actataaaaa aactaaaaag tggagaacgt tcaatagaaa aactaaattt acatgatgct
      421 atttgcctca ctgaatttgg tcttaaaaat aatagaaaaa atgttgaaat caacatatgg
      481 aaataattct taatcagctg cttttgtcaa aagcacatag gaatttcacc tctctccaag
      541 tttacggcga gccttacgga agtatcatta tcccaacttc aaatcatcgc tttttattga
      601 ctataaaaat tattttagaa tgtttatcgc tccttcttta cagtaagaaa tcatggcgca
      661 actaaaatat gctccttgaa gtgttaacgt actgatagaa tgtatttaaa tttcaatgat
      721 caataggttt tctgtttaaa agaatctact aatcctttaa ctattagaag attcttttaa
      781 acagaataag tggaaagggg actttttagt tagttaattt taaattttaa gatcgcttct
      841 atcaatttaa tttctagctg tcttagcata tactcatcta cataggtaat aaattgcccc
      901 gcctcattaa atccatttgt caaacataac ttagctatat aactatgata atgtttgagt
      961 atcatcttga ttgaaattgg atcttcttct agagcagatt ggataatgga tagaggaact
     1021 aatggatact gccgtttttt ggggttgtct accataagtc ataccccatt tttaattgtt
     1081 caactgcctt ctttcgttga taccagatac cacttgtact catattgagg attttaccga
     1141 tttcagtatc attaaaacct gcaaaataat aaagtaggat aacctttctt cttatttccg
     1201 ataaatgatc aatggctgcc gataactttt gatctgaaat taatatttcc attccaaata
     1261 agaaatagac atcttgttca tcaaaatgag tttgggagta cttaccttca tttgaattcg
     1321 tcaatggttc ctcattatct gttatgttaa tgtttcgttt tcgaatagac ttagtttcgt
     1381 ttcgtaatac ttttttacag taggcatcaa attgatgttg tatagatttc tcattgtaat
     1441 tttgttgcat gataaatatg ctcctttttt cccctttcca ctaatacaac aatcagaaag
     1501 cgcctttttt tcgaaaaaac tagaaaaaaa tatccttctt taaacagaaa atctttttat
     1561 aaaaaaaagg agttggcaat taagcagcca ctcctttttt atattcctta taaaaggtaa
     1621 attatttttt ataactgaaa tttggtattg atgaccatcg tttctttaga tagtgagcaa
     1681 tcattttagg ctttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa
     1741 tcccttgact tgtttcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattgtcac
     1801 atttatctat tacttcgtga tttgcttcgt aataacccac ttgatactct tctgtaaaca
     1861 ttgttggtac tgtatcatga agtccagcta ctgtatctgc tccatattct gtaaacatta
     1921 ttggcgtatc gggacatctt tccatccatc catctagctc ttttttcaac agatattttg
     1981 catgttttag gtctccacct gaaacatacc aaccataata gcgatttaaa cataaaacat
     2041 cgacaatatc tcctacttta caaatatctg gtgttgactt taagtaagta acgattgtta
     2101 caggtctctt ctgagggtca agttctttag tcaattcaac taaaggttca aaatattctt
     2161 tagcgccttc agaatccgaa tctggttcat ttgcaataga ccacattacg acactcggat
     2221 ggttcttgtc gcgttctatc aattccttaa tcgccatttt gtgagcattt tctgtccgta
     2281 tttctttcca agtatcatgt ttttctacgt tgtcatctaa taaagtagcc atgaaattca
     2341 aatgtaatcc tactgcaggt acttcatcaa taactacaat accttctcga tctgctaagc
     2401 gcataatttc ttctgaataa ggataatgtg aagtcctaaa tgaattagca ccgatccatt
     2461 tcattaaatt aaaatctttg atatttactg cttcattcaa accacgacca ttaatatacg
     2521 tgtcctcatg cttcccaaat cccttaaagt aaaaaggttt attattaatc aaaaacttcc
     2581 cattcgaaac ctgaatacta cgaatgccaa attcttctgt gtacgtatca atcactttat
     2641 tttcgttaat taaactaact ttcatactat ataagtaagc atttagaggc tcccaaagtg
     2701 ttgggttctt aagttgaatc actccttctt tccccttagc ttctgctata agttgagctt
     2761 cttcgtcata gattgctatt agaatttctc cggtccattc tgtatcgata ttaaattcta
     2821 catttgcatc tgaatcaatt atttttgtat taatagtgac atcctttata tagtctttcg
     2881 gagttgtata caatttaacg ggtctattaa tgcctgcata attgaaaaag tcaaaattaa
     2941 ccaaattttc tttttttatg tctccatctt cctgtatcgt ttctttcaaa atccctacag
     3001 gtaaagtgct atcgtcaata acattgttta cagccactgt aattctgtta cttccatctt
     3061 ttataaactg attaatttct gattcaaatg gcgtaaaccc tcctttgtgt tgattaatca
     3121 attttccatt caaaaatact tttgcttcat gtgttgcaga tccaaaacga agaactaatc
     3181 tttcttctaa taatttttta ggaagaatca cttctttttc ataccacacc catccaacat
     3241 gatttctaat gttctctgtt tcaaataaat cattataaga agaaggaact gaaattggat
     3301 aactctcttt ttcaaccatt ggttctaaag caagttcttc ttgtaagtca tcatttgtat
     3361 ctaacttaaa tctccaaaga ccgcttaaat caattacttg cctcgtctct gtcataatgg
     3421 gatataacat gtaaatcact cctatataat ttaaatatta aataaatgta tcctttaacc
     3481 cacaataagc atctattgga cttattccct taaattcaga aaggcccatt agtttgtcca
     3541 ctaatatttc taaagtttct tgttttccat cataagtatt gatataagtt tttacttgag
     3601 gcacatcagc taaatgaaac ggacactgta cagatacaaa aattgttgga atttcatgaa
     3661 tataaaatgg aatatccgga gtacctttac ttgctggcca aacaattctt tgaactgtac
     3721 ctgggttaac attagctata ttaataatca agtcatattt ttcagttaaa tctttgattg
     3781 gtctcttagc agcatatatt ccagctatat ttttaatttt ttcttcttgc gataatcctt
     3841 tcaattcttc ttcaggtgaa atgtattttt caacttcaaa tccttttgct gacaaaatct
     3901 gtttcaataa atcaatggcc tgaggtctat ttccagcaac tacttcacca aacccacctt
     3961 ttaccccatg tacatctacc agtaaaatcc ttttatattt ttctggtaca ataggccaaa
     4021 tatcttgttt atctttaact agtgtaattg cttgatcagc tacctcttta aaaattgatt
     4081 tattttcaat taaaccaatt ttttccatag cttcttgttt cggtaacaaa attttattct
     4141 tttctttttt atgcaaacct agtttagcct ttaagcctaa aattcttctt aatgcatcac
     4201 gtaaacgctc ttctgaaata attcctttgt aataaccttc catcatataa ttaaagtctt
     4261 catctggatc attaaaaaat aggaatagat cacatccagc cgcaatagaa gtgggcaata
     4321 attctgaccg tttcattgcc ccagtcattc ctaacatatg actagcatca gtaactacta
     4381 atccattaaa cccgagtttt cctctaagta agtcagtgat taattctttt gataaagttg
     4441 caggaagtaa atcttcatct ttcagatcaa gattaaaatg ttttgaataa gctggcatat
     4501 ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag tatacttttc
     4561 caaaagaatt atcccattct tctgtactta aactattaat actaaaagat agatgttgat
     4621 ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt ccactctctt
     4681 gaataccttt catataagct aaactaagtt ctaaaacttt atcagggctt tgtccaaaag
     4741 atcgactaga aatgatagga tttctccaat ttctattaat atctacaata ggagcaaaac
     4801 tccagttaca tccaatagcc gatgcttcaa ttccagaaac tctccctaat tcataagcat
     4861 atttcgcatc atttgttgca ccaattttta cttctacacc aacttcggta ccatctgtac
     4921 aagctccgtt accacctgct tctgtattag cagcaattaa taaaggaatt ttgctttctg
     4981 tttgtaggat atagttttga tcatatacct catcagcagt tcctggatta tatcgtacag
     5041 ctccaatatg gtactgattg accatactac ttaagtaatt tttatcacga ctagatccca
     5101 tattaacaaa taattgacct attttttctt ctaaagtcat agcatcaatc gtctcttcaa
     5161 tccaagaaat agcatcttca tctaaatgat atggtttttt tcgtaagtct acttttata
//
LOCUS       DAJSNV010000130         5182 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSNV010000130 DAJSNV010000000
VERSION     DAJSNV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.134"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            13..1020
                     /locus_tag="ON516_002580"
     CDS             13..1020
                     /locus_tag="ON516_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3346027.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1032..2036
                     /gene="neuB"
                     /locus_tag="ON516_002581"
     CDS             1032..2036
                     /gene="neuB"
                     /locus_tag="ON516_002581"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3346028.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2033..3181
                     /gene="neuC"
                     /locus_tag="ON516_002582"
     CDS             2033..3181
                     /gene="neuC"
                     /locus_tag="ON516_002582"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR3346029.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3154..3831
                     /locus_tag="ON516_002583"
     CDS             3154..3831
                     /locus_tag="ON516_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3346030.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3821..4963
                     /locus_tag="ON516_002584"
     CDS             3821..4963
                     /locus_tag="ON516_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3346031.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4976..>5182
                     /locus_tag="ON516_002585"
     CDS             4976..>5182
                     /locus_tag="ON516_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3346032.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCT"
BASE COUNT         1921 a          669 c         1025 g         1567 t
ORIGIN      
        1 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
       61 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
      121 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
      181 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
      241 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
      301 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
      361 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
      421 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
      481 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
      541 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
      601 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
      661 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
      721 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
      781 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
      841 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
      901 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
      961 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     1021 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     1081 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     1141 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     1201 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
     1261 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
     1321 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
     1381 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
     1441 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
     1501 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     1561 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     1621 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     1681 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     1741 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     1801 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     1861 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     1921 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     1981 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     2041 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     2101 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     2161 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     2221 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     2281 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     2341 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     2401 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     2461 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     2521 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     2581 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     2641 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
     2701 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
     2761 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
     2821 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
     2881 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
     2941 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
     3001 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
     3061 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
     3121 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
     3181 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
     3241 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
     3301 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
     3361 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
     3421 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
     3481 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
     3541 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
     3601 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
     3661 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
     3721 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
     3781 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
     3841 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
     3901 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     3961 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     4021 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     4081 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     4141 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     4201 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     4261 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     4321 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     4381 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     4441 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     4501 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     4561 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     4621 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     4681 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     4741 cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca
     4801 tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc
     4861 agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga
     4921 acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa
     4981 cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca
     5041 ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga
     5101 aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat
     5161 aaattcagtt tttgtttgta ca
//
LOCUS       DAJSNV010000131         5165 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSNV010000131 DAJSNV010000000
VERSION     DAJSNV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.135"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(62..520)
                     /locus_tag="ON516_002586"
     CDS             complement(62..520)
                     /locus_tag="ON516_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346033.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(533..1864)
                     /locus_tag="ON516_002587"
     CDS             complement(533..1864)
                     /locus_tag="ON516_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3346034.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1875..3317)
                     /locus_tag="ON516_002588"
     CDS             complement(1875..3317)
                     /locus_tag="ON516_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3346035.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3304..4068)
                     /locus_tag="ON516_002589"
     CDS             complement(3304..4068)
                     /locus_tag="ON516_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3346036.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4172..5047)
                     /locus_tag="ON516_002590"
     CDS             complement(4172..5047)
                     /locus_tag="ON516_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346037.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1565 a         1095 c          753 g         1752 t
ORIGIN      
        1 tcaaaaaaat tttgtaaatt cacccataaa tatcacccta tttaactttt ttcatcgtac
       61 actaaaattc aaaacaacta tctttaatct ttacttcttg gttttgtttt aataaatttc
      121 cttcaaaaat gcctggagaa actacaatga tcaccgtatc tttttgtgct tgctccaact
      181 tcttcaaatc tataatagct aacagtgtcc cttcttttac ctcctggtta ctttcaaccc
      241 gtatctgaaa cggacttcct tcgagttcaa ctgtatctaa acccatatgg atcagtattt
      301 ttctgccaga ttcactttcg atcgttatgg catgtcgggt aggaaatata ttatcgatta
      361 ttcctttaac tggcgcatgg atatatccat catgattcgt tatagcgtat ccaactccta
      421 tcatttttga agaaaagacc tcgtctttta ctgctgttaa tggaataatt tgaccactag
      481 ctacagcttt tagaatgttt cttgttttaa agaaatgcac tttttatcac ctctacttta
      541 atgctggcca atattttttg tttactttga tcatttcatc tagtattttt tttgcgactc
      601 tggcatttgg tacggtttga ttcaatgtaa atgcttgtaa agctttttga taagaacctt
      661 caaagtaagc atctactagt aacttctcta ctgcaacttg tgcctccatc aatcctttat
      721 gaaaatcact gattggtttt cttagtgtaa ttggctccac accagttgca ccaacatatg
      781 ccggtatttc aacaactgca tctgaccgta gattcggaat cgccccttga ttaggaacga
      841 ttagcataaa gcgacgacga tcatcattca acaaggaaat agctatatcg acgatatatt
      901 gtccatgctc cccaaaatta aagttcaata cttcttccac aataccgcgg cgtattttat
      961 cagccatttc tttggtattt ttctctcgtc catccatgac catattagca cgtgtatact
     1021 ctttgtcagt atgctcaacg accatatcag gatataagta atactctaag taattattcg
     1081 ggatattact tggaaaattt ttcgtgatga ctgacatcat ttgaaaggtt ttttgccaag
     1141 ttttatctcc tatattaaaa tcggcgactt gcatttcttt tgttattagt ttttcaataa
     1201 tctctggcat aatttctcgt tgcaattctt tatcataaat cgatgtatac cagccaaagt
     1261 ggtttaaacc ataatacgta acgatccaat tcttacgatc ataaccgtaa ttcactgcaa
     1321 tcgtttcttc aattgaaata gtcatatcac aggcattaat catttttaca ttcggatatt
     1381 tacgacgaac agcttcagaa acgatcgact ctggatttgt ataattcaaa atccatgctt
     1441 tgggggcata gtcttgtata tgatcgacaa tttccaataa acctttgatt gagcgcatcc
     1501 cataagcaaa tcctccgagt ccacacgttt cttgtcctac taatccatgt ttaagtggaa
     1561 tcttttcgtc cttttctcgc atctccaatc ctccaacacg aatttgtgag aaaacaaaat
     1621 cgcatcctgt aaaagcaagc tttggatcat ccgttgcacg aatgcgaata tttgaaaaac
     1681 cttttctttt cagcataaat ttaacaatca agtacatatc catatttctt tcttcatcaa
     1741 tatcatataa acgtatttca gataatggca accgtttttc attgttcaaa atggcttgaa
     1801 cgattcctgg tgtatacgta ctcccgcctc cagctatagt gataatctgt tttttcatca
     1861 tcatcttttc ctttctattc ttctaataat ttattgaatt ctgccataac ataagtcact
     1921 tctaagccga tgataatttg ataatttttt tcagttggtt taatgatccc gcttatccct
     1981 aattgcttga ttcgctgttc ctcgaccatg gtgggatcca ctacctctac ccgtaggcgt
     2041 gtattacaac tatagacatc caggatattg gcttttcctc caaacgcttg tagcattttt
     2101 gttgccatat attcataatt tttacttgct aaccctaact ctgtttcttc taaggttact
     2161 tcattgccaa actctgcctt agcttcacgt cctaatgtct gaatgtttct cttattgatc
     2221 aaataataaa aagtaacgta atacaaggca aaaaagaaaa ttccgattgg aagaatcaaa
     2281 atagcattgg tagcgattcg aaaattgatg agataatcaa caatacatgc tccgaatgta
     2341 aaaccaattc gtatattgaa taagtaacag acaatccctg ctaaaccggc aaaaagtgca
     2401 tggatcacgt acaacatagg agaaagaaac atgaaactaa attcaacagg ctcagtgata
     2461 tttgcaataa atgaagttcc tgcaccgctt cccatgattc ctttgaccat ctctttattt
     2521 tctttataag ctgctttata gatagctaga catatagctg gtataccaaa catcattact
     2581 acaaaaaaaa ctgataaaaa cagtccagcc tgaggatctc cgttgataaa gcgtgtcatc
     2641 tctcctctga aaacttcctt gctaggagat gtatagcttc ctaaatcgta ataaatatac
     2701 gtattcaaca catggtgcag tccaaagggg atcagcaaac gattcaaaaa cataaatatc
     2761 cccacaccta atgctcccat atcaacgagc agttttgcaa aattatcaat cccaatttgg
     2821 atgagcggcc agataaagcc caaaaaaaca gctgcgattg tttgtagaat catcaccata
     2881 gtcaatggga atttttcgcc cgcaaaataa gaaaaaacta ctggtaattt ttgttcttta
     2941 aagcgattat acgtccatgc cgctactaaa ccggaaataa ttccaccaaa aatccccata
     3001 ttcacttctg ggtccataat ttctaagcct tttttcaagg tagcaatcgc taaaaaacca
     3061 gtaagtgcag gtattccttt gtccttattt ttgacaaaac cgattgcaac tcctatagca
     3121 aataagatat ccaagtttcc aaaaacggca tttccagctg caccaattac tgggatgttc
     3181 agcatatcgt cagctgataa acgcatgatc aatcccgcta atggcatcgc tgcaactgga
     3241 agtagcatcg ctcttccaag cttctgcaca tttgatttaa cattcatttt tattcctcct
     3301 ctattaaata tcctcatatg ttactaactc aatcaaagga tcatttttca ctttttgagc
     3361 aaatgctgaa tgaattaaaa attcgacttg gtgggtacga ggatacacaa aacttgaacc
     3421 atttaacaat tcctgatcca aatacgctgc gtgacacatg acttcgatcg ttttatcttc
     3481 tctcaatatc atgagtaaac ttgccaaata ttcttccaca ctcgtattga tttgtcgttt
     3541 tttttcatca gtttctccta cgacatcgaa acaaggctca aaataatcga catgacgaac
     3601 ttcatctttt cgttcaaagt tgctccgaac cggcaaatca tatttttttg ctaatgtaac
     3661 aactacctcc tgattttcat ccaaagtatg agtatgatga tgactgtcta aatgagttgg
     3721 tatgatcccc gcatgataaa ttttttgaat ctgagcattc cattcctgat acagttgatc
     3781 tttgtcaatg ttaaatggat gctgataata agataaggat ctgaactctc cgctttcgat
     3841 aagagtatcg acagttttga ggagtggttt gccacacgtc aatgttaaat ggactcctac
     3901 acctagccta ggcatttcct ttcttagttt cacagcatgc tcgaaacccg gcatatttgc
     3961 catcaatgta gttgatgtca aaattcctcg ctgataagca tccataatcc cataattcac
     4021 accatggcta tagccaaaat catccgaatt aaaaatcact tttcccattt cattctcctt
     4081 cttttttcta ctaaaatagg cgcaaccaaa taatcatagt ccatttacta tgtgtcattt
     4141 ggttgcgcct atagatagtt acgcccttat tttaatagat tcttgctaga tgaattgcca
     4201 aatagatgat ttcattttct tgaatggcaa gttgaaattg ctgttcgatg atatctttaa
     4261 tctttagtgc tgtacaatac gctttgctat gtttaaatgt ttcagtaaaa aaaccaatat
     4321 tttctactgg cgtttcttcc tgataaatca agcgtttaac caggaatttg atatgaacca
     4381 ttaagcgatg ataagtcagt gattctttat cataaacgtt tgaataggat ttttctagat
     4441 gggacaaaat cgtggtcgtc atttctacta tttttaagct ctttaaacta ttaggctctc
     4501 ctagctcagc attaatgata tgcatagtga tgaatcctac ttcatttgac cctaactcgg
     4561 tttgatatcg atgattgatt cgctcgacca cttctactgc tatatcatat tcatttggat
     4621 aaaggaatct catttctggc aacataattg ctgtgacatc tctatcttct ctttttcgtt
     4681 gaatagcaaa ataaatatga tctgtcaatg taatatagat atttgaatct aatggagagg
     4741 agatcctagc tgttatcagt tcaatcactt cttccgcaaa atccataaat tcgattggaa
     4801 tctgcgagac tatctcactg aatcgtttga tcttttcttt agaatctaat acaaataatt
     4861 tgtcagcatc ctcaacatca aatggcttac tcactttagc agaaaagcca atccctttcc
     4921 ccatcgcaat tttttctatt ccatcgtcat cgatcaatac agcattatga ttcagctttt
     4981 tcatcacata caccgttaat tcacctcctc tatatgataa aacaaaacaa cttgcagact
     5041 ccttcataca tatttttgtc tccaaacaat ttttaaagac aaaaaacgac ctaagaaaga
     5101 accctttctt aggtcgtgcc taattgaata gtagcaccct acataattta caaaaatagt
     5161 ttaac
//
LOCUS       DAJSNV010000132         5062 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSNV010000132 DAJSNV010000000
VERSION     DAJSNV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.136"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            8..619
                     /locus_tag="ON516_002591"
     CDS             8..619
                     /locus_tag="ON516_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3346038.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            692..1621
                     /locus_tag="ON516_002592"
     CDS             692..1621
                     /locus_tag="ON516_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3346039.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1690..2516
                     /locus_tag="ON516_002593"
                     /pseudo
     CDS             1690..2516
                     /locus_tag="ON516_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2564..3742)
                     /locus_tag="ON516_002594"
     CDS             complement(2564..3742)
                     /locus_tag="ON516_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3346040.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3840..3938
                     /locus_tag="ON516_002595"
     CDS             3840..3938
                     /locus_tag="ON516_002595"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346041.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            3969..4370
                     /locus_tag="ON516_002596"
     CDS             3969..4370
                     /locus_tag="ON516_002596"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346042.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4577..>5062)
                     /locus_tag="ON516_002597"
     CDS             complement(4577..>5062)
                     /locus_tag="ON516_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3346043.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1589 a          868 c          980 g         1625 t
ORIGIN      
        1 aaaataaatg tattttatac tacctacact ttatagcctt ttatgtgtaa tggcaccatg
       61 tattatttat atggtatttt tttgcaaaaa aaataccctg aaacacaata tcctgatttt
      121 gatttttatg ctgtactgtt ggaaagtttt tgaattgact ggtacaggtg gactaacaga
      181 tattttttat agacctgata caggtaggaa attttctcta gtacagggca gtgttaatct
      241 tgtaccattc agtggactaa acattagctt tatattaaat atagtaatgt gtgtgccctt
      301 aggattctta ctaccaatca tatggaaaag ttataggaaa atagttcctg ttttcttggc
      361 tggggctcta ttttcactaa ttattgaagt ctcacaaatt ataactacaa gagcaacaga
      421 tattgatgat ttaatagcaa atacctttgg tgcggttata ggctttatac tgtgggctgt
      481 tttaagtaga gtggtgtcct tgaaggacaa gtcatctaca gtagattatt tagaacctta
      541 tatttatata ttgattggtt ttaccggaaa attcttactg tattttcctt tctggtttgc
      601 ttttgaagta ttatcctgat aattccatag aaaataatga gtttgagttg agaaagaata
      661 ttcttgtaca caactctgga aaggaggaag tatgaaaaga ttcttaaaat gggggcttgt
      721 tcttttagcg tctttgattg tgattctaat gtgtacagcg gttgttattt cttatgtgaa
      781 tcggccgtat gcaagagctg tttatgatag gattaaacgc attcttgaca aagattttta
      841 tgaccatcta tatctaggtt cttcatttga aataataaac cttgatacaa tggatattca
      901 tagctatatg aaaaaagacc aagaagaatt acccgcctca ttgagcaaat tatttacgat
      961 tgattatgcg cttagtgtcc taagtttaga tgaacagatt acaattagtc ctactgcact
     1021 tgacatgcag aaagctgact catctactgc acatcttcaa gaaggagata ttttatctgt
     1081 ttatgacatt atctgtgcta tggtcttacc ctcaggaaat gatgcagctt atagtttggc
     1141 aatttataca ggtggaaaac ttgatcctga tgcaaagaat gatgaagaac gctatcaaag
     1201 cttcatgaaa aacttagatc gttatctaag tgagcaaggc tataaacata ctcattttac
     1261 tgacgctgct ggctatgact ccaatgctac aacatcagcg gaagatttga caaaagtagc
     1321 tgcaaagcta ctaaagaatg ataaatttgc agaaatagtt gccaaggaaa cttatagagc
     1381 aaaatcaatt aagggaaatg tttataactg gaatactacg aatcaattta tgcaacaaga
     1441 taatagatac tttactgaag gtgtaaaagg tgtaaaaact ggatcgtttg atgggagata
     1501 caatctatgt gtgtattata catctagttc tgataaccac tatttacttg taagttttgg
     1561 ttcagagagt gatagtaggc gttatgtaga tgttcaaaat ctattgcata atctgccttg
     1621 aaatttcgtg cctttaagcg cgtatctaaa gtttctttga aaagtttgag aatgaagaaa
     1681 gtgagcagtc gaaaaacggt tgctcacttt tttgccctaa aatcgaaaaa atataaaaaa
     1741 gtcctttaca acacgtctca ggaaaaactg ctaagtcaat tctaatatct tgtggagcaa
     1801 gacttgcacc ttttgatata agagagctta gagaactaat gagcgaagat gaactagaac
     1861 tcgataaaat tggagataga aaaactgcct tgtttgtaat aatatcagat acagatgata
     1921 catttaattt tgtagtttca ataatgtatt ctcaattatt taatctacta tgtgataagg
     1981 cagatgatgt gtatggtgga agacttccag ttcatgttag gtgtctactt gatgagtttg
     2041 caaatatcgg tttgattcct aaatttgaaa aactaatagc gacaattcgt tcaagagaaa
     2101 tatcggcaag tataatactg caagcacaat ctcaattaaa agcaatttat aaagaccatg
     2161 cagatacaat agttggtaac tgtgactcta cactttttct aggaggaaaa gaaaaaacaa
     2221 cagtaaaaga attatcagaa accttaggaa aagaaaccat agacctatat aacacatcag
     2281 aaacaagatc aaatcaaaaa tcttttggtc taaattatca aaagactggc aaggaactta
     2341 tgagtcaaga tgaaataact gtaatggatg gtggtaagtg tatttaccag ctaagaggag
     2401 caagaccttt tctatctgat aaatttgata ttacaaagca taagaattac aaattgttgg
     2461 aagattatga taagaaaaac ttgtttgatg tagaagagta tttaaaacaa agatagcgta
     2521 aaatattttg tgtaaataac tcgtccttct gcaaaataat gggttactca gtaaacattg
     2581 aagctaatgt atccgtcact tgttggaagc ctttatggct tctatttaag aatttttgat
     2641 tgtatgtatc aaagatggat actaggaaac gctctagtga ttcttcattt tgaaactgct
     2701 cttttctgtg gctgtatttc ttgatttgct tattaaagga ttcaatcaag ttcgtagagt
     2761 aaatagtctt gcgtatggct aatggaaaat catagaaagt gagcaggtaa tcgttcgaaa
     2821 ggatcgattc caccattttt ggataactgg ttttccattt ttccgcaaaa gcaccacgtg
     2881 cttccaatgc cgccttttta gatgacgctt gatacaccat tttaaaatca tcacagactt
     2941 ccttacgatc atcgacacgc actttgtgac tgatattacg ggaaacgtgc acacagcaat
     3001 gttgaaaacg ggctttgggg tagaaccgct gcacggcccc tgccatcccc tttaagccat
     3061 ccgtgatgaa aaggaggacg tttttcacgc cacgttcctg gaggtctatt aaaatctctt
     3121 cccaaatcgt gatagattca gttggtgcaa tcgcatagct caacacttcc tttgaaccgt
     3181 ccgggcgaat gccaactgcg atatgaatgg cttccttggc gacggttttc cgctttaaag
     3241 ggatataggt tgcgtccata taaatagcgg cataacggtc atgaagctca cgttccttaa
     3301 atgaagtgac ttcttccgta aatgatttag tcatattgga catggtttgg ggcgtgtagt
     3361 gatgcccata cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga
     3421 agaggtgaat gacggtctcc tctaaggtat cattcgtccg tctataagca ggaacagtct
     3481 gttgcttgaa ctcgccgttg cggtcgcgcg gaatctggag atgaagttcc ccgtactcgg
     3541 tcttgaccgt acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat
     3601 cgtacttttc gtaatcgagg aaagccgtta actccgtttt gagaagcgta ttgacagctt
     3661 tttccaaatg gacacggaat aattcattca aatcgccttt attcgctaga gtctttagaa
     3721 tttctgtagt aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact
     3781 ctattctact gaagggactt ccttttttct atttacacaa aatattttac actctcggat
     3841 tgaaagaacg cggtctttca ggagtaaaga tggtcatctc ggatgcgcat atagggctca
     3901 caaaagctgt gaaagaatac cttctttgtg cgagctagca gagatgccaa gcacatctcc
     3961 ttcgcaacat ttccgacaaa ttctccaaga aaatctcaag cgatatcaaa gaagacttta
     4021 aagccatctt caaaacggca agcatcgagc tggctggaga acgtaaacaa cttattatgg
     4081 ataaatatgg ggaagacaag aaagtcgcaa ctgcctgtaa aatattggat gagggcttcg
     4141 aagatgccat gcaagtaatg gttttaccag aaaatatccg tcaacgattg agaacaacga
     4201 atgttcttga aagactaaat gaagaagtac gccgtcgtga acgtgttgtc cgaattttcc
     4261 caaattgtga ttctgttatt cgtatcatcg gtgctttatt gatggaaaag gatgacgaat
     4321 ggacttctgc acctagaaaa tatcttgaat ttgatcgtaa cgagatttag cacattaagc
     4381 cgccaatagt ataatatact ggtgagaagt atcaagagac gcttcgcttt ttgctcttga
     4441 cacttcttac cagcatattg gaccgtagct aggctcaaca aggcaaaaca tcgtactttg
     4501 agatttcctt tttacacaag attttggact tgacacagcc cctatggttc ttaccttgtt
     4561 tatgcttatt taaatcttac tttattacag tactttcaaa cttcgttttg taagccgggt
     4621 attttgatat ttcataatta tgtccctctt tttgaatata ttctttgata tctgcttgtt
     4681 catctacact ttctttgcgt aaaatacgtt cgatcattga gcagcagtgc atgtaaagca
     4741 agaacaattg attgtttgaa aattcgatcc ccattagttt ttcgagttct ttgacggcct
     4801 tttgtaccaa tctcaaaacc ttcattgcat caaggatcgt taaattttct acgatcttgt
     4861 taatcgagaa attaaagtat tgagcacaat tataggcgat aaatggagca tttgattcta
     4921 tcacctgttg ttccttggcg tattcataaa ttttttctgc caaaaaggtt tttcctgaac
     4981 cgcttacacc catcaataaa atcggtaatc cgttatgagg atacaagaca gccgtcttga
     5041 tttgatcgat cgcttccttt aa
//
LOCUS       DAJSNV010000133         5029 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSNV010000133 DAJSNV010000000
VERSION     DAJSNV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.137"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..161)
                     /locus_tag="ON516_002598"
     CDS             complement(<1..161)
                     /locus_tag="ON516_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3346044.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHW"
     gene            319..582
                     /locus_tag="ON516_002599"
     CDS             319..582
                     /locus_tag="ON516_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346045.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            606..794
                     /locus_tag="ON516_002600"
     CDS             606..794
                     /locus_tag="ON516_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346046.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(877..1062)
                     /locus_tag="ON516_002601"
     CDS             complement(877..1062)
                     /locus_tag="ON516_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346047.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1277..1648
                     /locus_tag="ON516_002602"
     CDS             1277..1648
                     /locus_tag="ON516_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3346048.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1798..1870)
                     /locus_tag="ON516_002603"
     tRNA            complement(1798..1870)
                     /locus_tag="ON516_002603"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1835..1837),aa:Gln,seq:ctg)
     gene            complement(1916..2344)
                     /locus_tag="ON516_002604"
     CDS             complement(1916..2344)
                     /locus_tag="ON516_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346049.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2404..3162)
                     /locus_tag="ON516_002605"
     CDS             complement(2404..3162)
                     /locus_tag="ON516_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3346050.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3457..3654
                     /locus_tag="ON516_002606"
     CDS             3457..3654
                     /locus_tag="ON516_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346051.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3760..4242
                     /locus_tag="ON516_002607"
     CDS             3760..4242
                     /locus_tag="ON516_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346052.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4300..4896)
                     /locus_tag="ON516_002608"
     CDS             complement(4300..4896)
                     /locus_tag="ON516_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3346053.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1636 a          788 c          966 g         1639 t
ORIGIN      
        1 tcccaatgcc taaaagaaaa aatttgataa gtaaaatata cagcaggtaa agaggccagt
       61 aaaactgaaa acttcttttt tgtccatttt atgtaataga aaagaatgct tatcataata
      121 aaaaaaggaa tataaaaaac aaattgaccg atcgcgatca tactattgcc tccgataaat
      181 aaagtccatg atacaaaata acactttgtg cgaaatggcg caagcagttt tgtggattta
      241 tccggtaaaa tctgtatcga ttttgaaatt agaggagaaa cttgctataa tcaaagtgaa
      301 agaaggagtg ggattttcat ggatcatgta gtgttagaaa tcgatttgca tttgatcaat
      361 aatattcggg tgatttacta tgttgtaagt aacagtgtcg aacaacgtgt actgacgaat
      421 aaaataaatg gtattttagc aaagaaagac gttcaccgat tcaataatgg tgaaggaagt
      481 tattatagta tcccagtaga aaaaattatt tatacgacag tcaaagtcag agaagatcta
      541 gataccaaaa aagcatatga acctattttt acaacttatt gattgacagc agatggagga
      601 aaaaaatgaa tccatatgaa caacttattc aagatttgat cgatggaaaa atcgaaaaga
      661 ttgatgtaaa aagagaagaa ttaatggctt ttcgagaagc gtggctgaag ttggagaatc
      721 gtaaatatgt ggtgggaaaa gcctcactga acggcaatgt gacttatcat tacgatccga
      781 cacgtttatt ttagtatgaa gtattcagaa aagaaccaaa ggaggacgat gcagcagcat
      841 tgtcctcctt tgattttaca aaagtgaaag ttttacttat ttgaatagat ttttaaattt
      901 gtttttgaca ctgtcgatat ttacatcttt taaaagattt tgggctttct ctttataagg
      961 tcctaattgt tctttatgtt cttcgataaa tcgttttgcg ccatcgaaat tgccgttctt
     1021 aatcatttcc ttgacttttt gaaataattc ttcttgattc attttgaaca catcctttgc
     1081 tatcaacatg attttttaca tggacaaaac gagaaaaact gatttatcca cacgtcaata
     1141 atatcaaata acgatcattt ttactaaaaa aacactcgat tctcgcttct aaagtaggag
     1201 aaaaatctcc aacttgggac ggatggaatt tctttaactg acccatatac tgaatatata
     1261 acaaggaggt agaaagatga aagtgattaa atatttgatt ggactggcgg cgattagtgg
     1321 tattttgcta tttggactga aatattatac tgaaggaagt tatggagaaa tccctggtat
     1381 acttgatcag ctaaatccat tagttgaaaa aggagaggtc tatgtaaaga cacaaaaacc
     1441 agaggaagta aatgaatatg gtacagcaag gtatgtgcag aaagctgctg atgccaatgg
     1501 gaaagaacga acggttgaat ttaatggtct ttctgtgtta aaagaaaatc attatttgaa
     1561 aattagcaat aaaggagcac acatagaaac gtatgaggaa gttcctagag aaaaagttcc
     1621 ggagaaagca ttagcaatta ttgggtaaat cagtttttgt attaagaata cgcccgattt
     1681 tattattcat aatttaatta tgtaaactaa atacaagtag taaaaataat acaataatcc
     1741 ccctgcaaaa agattgttct tttacagggg gttcattttt tcagcaagca catcaaattg
     1801 ggatggcagg gttcgaacct acaattgctt gattcagagt caagtgcctt accagtttgg
     1861 ctacatccca tttattcaac aaaataatca tagcactttc ctaaaaaaac gcaagttatt
     1921 tttcttgatc ggatagttgt tgctttactc gtaaaaggcg gcgtactagt ttcttttcta
     1981 atccagcagg aagggcaatc agttctgcta ctgacaagac ggtgtccaat aactgatatt
     2041 ctttgacagg ttcattcata atatgataga tgtttgctaa acgttcaata tattcaaagc
     2101 gagtatactc tgttcgagca attagaaatt tcaataaatc ggtggctttc tcgtactctt
     2161 gatctgcgta ataaacaaga gattgacgat acaatttgat ttccagttca ttgagggtag
     2221 caggtctgcg acgctcgttc cactcattaa tagagacttt accttgaaca tttcccttgc
     2281 agtcacggac aaaaataatc ggaatttcat ttggatccat ttttttatgt gtgaattttt
     2341 ccattatcta cgctcccttc aaaggtttgg tatcttgagt ataagggatt tttgtttgaa
     2401 ttactagctt ttctccaaag ttagcatctg tccacccatt gcagtgaaaa tcgccgaaaa
     2461 atcgttgatc ggtactgctc gattttttac accataggga tcgttgacat aaatattttc
     2521 tttgtcatat ccagtgacta cacaagcatg aacaagagga gtgactctta cttctccatt
     2581 atttgtggtc caagtcataa aatcagtttc atctgggact tcaaaattga tagtcacaga
     2641 agtccagaca ggatttcctt tagatagtgc ttttttgata tcatcgagtt gagcatcggt
     2701 ttttggaaaa gtgtgataat ccgtttggac gatttgctga gcaagggagg caactggggg
     2761 aacatagacg accatagccc agtctccgcc actgatatca ccaacaaatc cttcattagg
     2821 atcgccatga gtcccatctg cattgaaaac agggacgtaa tccaattgct cagctaactg
     2881 gtttttagtt gtttcataac cataaaaaga taaaagcata gaaagcgaag tgacttcaca
     2941 gccattttct aaagaaggag aatcaaactg gctctctaat ggaacatcta gtaaaacttc
     3001 tgctttctct tttgtctttt tcgttttttg tgttgaactt ttttgtgcag tgttcattgt
     3061 atgttctgga gaatggatat gtaggtaata acttgtaccg cttaataaaa gagcagctgt
     3121 gccccctaga aacaatagtt ttttgaacgt ttttttcttc atttgaaatc tcccttttca
     3181 gctgtttatc tttcctatta taaatgtatt tttattggaa atggaagtat tatattttaa
     3241 tatgtaggaa ataattagtt tttaaggaat atgttttaat tttttgaaaa aaattcttct
     3301 aaatacaaca gctgttttaa ttgtgaaaac gaaaacagta cgctataatg cctgtaaggt
     3361 tgatttttaa ctgtaaataa aaatcaatga gttaaatctc caaagaaata gcagtggtaa
     3421 tgtggagaaa agttccagca gtttaggagg attttgatga cggaagaaat gattaattta
     3481 ggagaacaat atgcatgcaa accaattggt tttacaaaaa cggtcattgg tgaagttgta
     3541 tcaaaaatga caaactgcgc cgtggtcaaa gttgcccaat gtgctgcaga ggaccaagaa
     3601 ttattagatg aaaaagcaag catggttgtt gcaaaatatg atacatttga ataaagaaaa
     3661 caattggacg tgcggcgtgc tgcaagtcca atttctttga gaaggtaaat cagtattctt
     3721 ttcctatttt ctggagaaga aaaagcgagg cgagttgtca tggtaacatt ctttacggtt
     3781 ctattgtttt tattcgttgt tgtgatgttt ttatttcttt ggctggttcg aaaagaaatt
     3841 atttatcgga ctgtgcgaaa tcgatgggtc tacttggtga tacccttctt agtgtcacta
     3901 gtcatttggt atacactcat cagtcatcct acagcagatg aattggcaaa agggatcttg
     3961 tcagcaatga tttttattag ttttttgttg gacagtagag gaatcacaga agaaggactt
     4021 gttttgaata gctttgacaa aaaaggtgtt cctttttctg agatcaataa aattgtatta
     4081 tatcagccaa aaggttctaa aatcgtaaaa atgaattttt tccgaaacgg atggcgagga
     4141 ccgatgctga aattttctgc atcattagaa gaattagttc catttttgtc ccaacgctta
     4201 aacgatgagg cagagattga tattatgatt gatccagaat aaaaacagag gttgcgacaa
     4261 gatgtttgtc acaacctctg ttttttattt caaataaaat tatccaaata cgcggaagcg
     4321 atcggcatcg ttcatgtatt ctttttcccc agccggtgtt tctggtgaac cgaatactaa
     4381 ttgcgaacgt agtttccagt ttgaagggat attccattct tttgcaactg cttcgtcaat
     4441 aactgggttg taatgttgca agtttgcgcc caatccttta tcaactaatg ctacccaagt
     4501 attagctgtt gcgattccgt ttgattgttc tgaccaatct gggaagttgt ctgcatataa
     4561 ttcaaattgt tcttgtagac cttttaccac gtcagtatct ttgaagaata atactgtacc
     4621 ataaccattt ttgaaaccag ctaatttgtt ttgtgtattt gggaaagctt ctgctggagt
     4681 caatgggcga agtgcttctt cagtgatttc ccaaagtttt tcatgtgcat ctccgaaaag
     4741 gataactgcg cgtgttgatt gagcattgaa agcagttggg ctttctttaa ttgcttcttt
     4801 gatcaaagtt gttaattctt cattagaaag tgaaacatta cgtcctagat gatagattga
     4861 acggcggttt ttcaaagtgt cagtaaaagt tgtcataata aaatttctcc tttgatttgc
     4921 gttttgcatt tttttaattg attacgaaat gaagtatagc accttacaaa aagtaagtaa
     4981 acagatatgc tcacaaaaaa taagcgaagt tcaaaaaaca attttaata
//
LOCUS       DAJSNV010000134         5000 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSNV010000134 DAJSNV010000000
VERSION     DAJSNV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.138"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..863
                     /locus_tag="ON516_002609"
     CDS             <1..863
                     /locus_tag="ON516_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3346054.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="ON516_002610"
     CDS             1189..2040
                     /locus_tag="ON516_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346055.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="ON516_002611"
     CDS             2137..4017
                     /locus_tag="ON516_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346056.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="ON516_002612"
     CDS             complement(4057..4959)
                     /locus_tag="ON516_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3346057.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         1566 a          872 c         1125 g         1437 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt
//
LOCUS       DAJSNV010000135         4990 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSNV010000135 DAJSNV010000000
VERSION     DAJSNV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.139"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(274..2082)
                     /gene="pepF"
                     /locus_tag="ON516_002613"
     CDS             complement(274..2082)
                     /gene="pepF"
                     /locus_tag="ON516_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3346058.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2258..3256)
                     /locus_tag="ON516_002614"
     CDS             complement(2258..3256)
                     /locus_tag="ON516_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3346059.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3482..4135)
                     /locus_tag="ON516_002615"
     CDS             complement(3482..4135)
                     /locus_tag="ON516_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3346060.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4467..4865)
                     /gene="spxA"
                     /locus_tag="ON516_002616"
     CDS             complement(4467..4865)
                     /gene="spxA"
                     /locus_tag="ON516_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3346061.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1470 a         1033 c          778 g         1709 t
ORIGIN      
        1 attttcctcg aaatagatat ttaggtctag aatatttagg gtatctttga taagctgagt
       61 atgagacatc ttgaatcctc ctatgaattg gtagccaaat taattctaaa ggatttcagg
      121 gtgtcttttc tatatttttt tatacaaaaa agatgttggt gaagttcacc aacatcaaaa
      181 agtatagagc cttaaaaaaa tacgtcgact cccttacaag agagccgacg tatttttttc
      241 gtttctattc gagaaaatca aaaatagaaa gccttataaa cgatcgatca attcttctaa
      301 ttcatttaga cgttgttcaa acatcgacat tgcatcttca atatatgcag cctgtgtcat
      361 atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg cttttaggta
      421 agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg ccagtgctga
      481 agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa aatgcggtat
      541 ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat aatattttgc
      601 attcaattta ccataactat cacttaggta ttcactagtc aatggcacac ctttttcatc
      661 ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt
      721 aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt tttctgtttc
      781 caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat
      841 ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat gaacactatg
      901 tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat tcaataaaat
      961 atatggattt gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc ctttattttc
     1021 gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt attcttcacc
     1081 cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa aggtaattgg
     1141 agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca cttctaataa
     1201 acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac
     1261 caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt ctctggcaga
     1321 gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga
     1381 agcaaatgta tttctaaatt gttcgtaaac actgtacaac cccttaaatg ctgcttcgcg
     1441 cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata attggactat
     1501 ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca aaacagcaaa
     1561 tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt cctgctcagc
     1621 agataagaca tgggctcgat tatctactat ttgctggata taatggcgat agacttccaa
     1681 ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca atatttctgg
     1741 ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc ttgcataaag
     1801 cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat atacataaat
     1861 gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa attgagaagc
     1921 gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg ataattccaa
     1981 atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt
     2041 ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca ctccttttct
     2101 tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc acagcatcta
     2161 acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa atcttgtttt
     2221 acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa gaagacaagt
     2281 gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata ggaaaaagcc
     2341 attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga actttctggt
     2401 tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt aaataaaaga
     2461 aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta tgacgcaagt
     2521 agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg cgtgtcagcc
     2581 atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt ttaatttcgt
     2641 tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg
     2701 aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt tgtttcttga
     2761 gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa cttttttcta
     2821 tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat acttggtaac
     2881 tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt ctagaacatt
     2941 ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat
     3001 aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag cattccttta
     3061 actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct gatttttgat
     3121 gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt cgacagccac
     3181 gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta ttatattcat
     3241 tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat ttatttacac
     3301 gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc taaacaaaaa
     3361 tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc ttttgaacac
     3421 atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact ataatgctga
     3481 tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga cattgtccat
     3541 attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa attcgagcaa
     3601 tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg actaggtaat
     3661 agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca tctgttgcaa
     3721 gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta
     3781 gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga cgaagaaagt
     3841 cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct tcatccggaa
     3901 gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg aaagtgacag
     3961 cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta taaaagaaat
     4021 tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat
     4081 cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc
     4141 tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg
     4201 taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt
     4261 tcaagctatc ttatttaact ataacatttt tggcagacga atactatata gccgactttt
     4321 cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc tttttataca
     4381 tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag
     4441 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
     4501 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
     4561 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
     4621 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
     4681 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
     4741 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
     4801 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
     4861 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
     4921 taatctccca tctgtttcta ttatacataa tcaactttta aatagctacc tttttttcat
     4981 aattttttta
//
LOCUS       DAJSNV010000136         4896 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSNV010000136 DAJSNV010000000
VERSION     DAJSNV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.140"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(127..1275)
                     /gene="nagA"
                     /locus_tag="ON516_002617"
     CDS             complement(127..1275)
                     /gene="nagA"
                     /locus_tag="ON516_002617"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3346062.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1351..2187)
                     /locus_tag="ON516_002618"
     CDS             complement(1351..2187)
                     /locus_tag="ON516_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346063.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2371..3588)
                     /locus_tag="ON516_002619"
     CDS             complement(2371..3588)
                     /locus_tag="ON516_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3346064.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3737..3916)
                     /locus_tag="ON516_002620"
     CDS             complement(3737..3916)
                     /locus_tag="ON516_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346065.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3971..4054
                     /locus_tag="ON516_002621"
     tRNA            3971..4054
                     /locus_tag="ON516_002621"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4005..4007,aa:Leu,seq:gag)
     gene            complement(4294..4872)
                     /locus_tag="ON516_002622"
     CDS             complement(4294..4872)
                     /locus_tag="ON516_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346066.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1501 a         1012 c          786 g         1597 t
ORIGIN      
        1 ttacttattg aacaatagga aattaaagaa acttttgaaa cttatgtaaa gtgattttta
       61 ctagaacaca aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa
      121 attatattat gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc
      181 agggttcaat acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc
      241 gattttacag ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc
      301 taatccccag tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa
      361 gttgccagat tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt
      421 gtaatttcca tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc
      481 atgtcctgct ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca
      541 aatcaattca gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg
      601 atgattcaat ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac
      661 agctttttgc gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc
      721 ttcagtcact tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt
      781 tttgattaaa ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc
      841 gaaataactt gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata
      901 gattcctttg attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac
      961 atcttttaaa cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca
     1021 agataacaga ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg
     1081 attcatatag ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg
     1141 gtcaatgatg ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc
     1201 ggtaatttca agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata
     1261 gataaaagtt ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt
     1321 agagaagaag tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc
     1381 ttgtgctaag ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa
     1441 gcttttaagc acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata
     1501 gattccttga ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt
     1561 tccataatta taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg
     1621 cagcagttgg atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc
     1681 atatattttt ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc
     1741 tttttggcta gagtagctta atgcttcttt caaataataa agggactgag agatattttg
     1801 acgcaacgtg aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg
     1861 atacagattt tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg
     1921 tttttggaaa caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg
     1981 agagagattc atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac
     2041 caatacattc ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat
     2101 atcatgcgaa agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt
     2161 tccaaaggac tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt
     2221 ctattcgtat tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat
     2281 atactttagt tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg
     2341 ccctcctact gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc
     2401 tctcattttc ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa
     2461 atgatctggc tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg
     2521 taaaaagtcc aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt
     2581 tgggaatata taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat
     2641 tgcattatag atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc
     2701 gacactttga taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca
     2761 caaacgcatg gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc
     2821 tccacttagg accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc
     2881 gttcagcgta acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc
     2941 atccatcact aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc
     3001 tacttcaacg atttcttcta ataattcttt tggataaagt gccccagtag gattattagg
     3061 attaatgata acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc
     3121 aggataccat tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc
     3181 aatagaagcc gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc
     3241 cagcaacccc tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc
     3301 attgatcgtc acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc
     3361 agaaaagatc cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc
     3421 acgaatgact tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt
     3481 taaaatacta atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac
     3541 atcgtaactg acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat
     3601 ccctcatttc aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat
     3661 actccatcct agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca
     3721 aacaagcgtt gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca
     3781 ttgcaattcc acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc
     3841 caatcaatac acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct
     3901 taataagtcc tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg
     3961 ttattattac gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg
     4021 ttagctcgtg tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct
     4081 tcacgagttt ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt
     4141 tacaaaaaga acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca
     4201 agctactcgt agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct
     4261 cacctaatac taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa
     4321 ggtacaaata ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat
     4381 gcttttcatt agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat
     4441 atagtacgtc cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga
     4501 acaaaatccc attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact
     4561 taaataaatt ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca
     4621 agtacctgga taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt
     4681 actactactc caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat
     4741 tgtattacca aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac
     4801 ataatagttt tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa
     4861 taagatattc atgtctaaaa aaaacggctt tccaaa
//
LOCUS       DAJSNV010000137         4882 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSNV010000137 DAJSNV010000000
VERSION     DAJSNV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.141"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            228..818
                     /locus_tag="ON516_002623"
     CDS             228..818
                     /locus_tag="ON516_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HCR3346067.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            845..2155
                     /locus_tag="ON516_002624"
     CDS             845..2155
                     /locus_tag="ON516_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3346068.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2152..2871
                     /locus_tag="ON516_002625"
     CDS             2152..2871
                     /locus_tag="ON516_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3346069.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            2886..3971
                     /locus_tag="ON516_002626"
     CDS             2886..3971
                     /locus_tag="ON516_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3346070.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4107..>4882
                     /locus_tag="ON516_002627"
     CDS             4107..>4882
                     /locus_tag="ON516_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3346071.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGL"
BASE COUNT         1721 a          538 c          939 g         1684 t
ORIGIN      
        1 gttaggttta aataactgga aatatcggaa aatgggcatt atacatcacg gacatccctt
       61 cttttggaaa aaatgttacg atttttataa gtaatattgt atccggtgga atcaacgatg
      121 aagataacat atccatcgtt gtagattcag tagcattgaa tgatgctctt gttgcaagaa
      181 atccgaataa aattgttaag tataaattga attaatagga gatatgaatg gaaattagca
      241 gtaaagaata tttaaaagcg gttcttcgta tggaaaagaa tctatatata gaatctaaag
      301 gacaacacac aaaattacta aaaataatga aatcacatcc ggactacaac gcttggcggt
      361 atactcgtcg aatgagaatt acatcttatt attattcaaa gagaaagaaa aatatcattt
      421 atgcgattat gtatattgtg tcttgcagaa gaatgaatag tttgggaaga aaatgtggta
      481 tagagtgtgg tgaaaatgta tttgatgaag gtgtaagatt ttaccataca caaggaattg
      541 ttattaatgg taacgccata ataggaaaaa attgttactt atatgggaat aactgtattg
      601 gaaatgatgg aatcgatcct aagtgtcctg taattggaaa taatgtacgt gtatgcgtgg
      661 gtgcaaagat tattggtaat gtaaaaatag ctaacaatgt agttgttgcg gcaggagcta
      721 ttgttatcaa agattgtcta gagaatggtg caattcttgc aggtgtaccg gcaaaaataa
      781 tagggtatgt taatttaaca aaagtatttt tagaataatt tttagcgaaa gaaggtgaat
      841 aacgatgcgt atttcaataa aaagatggtc aggagcagat tggacattgt tcacgttggc
      901 attttttgca atctccatag ttcttgtata tacttttaac gccccaagag caattttata
      961 tttgggagat ataattaata tctttgtgtt attaaatgca cttagcttta gacgcagaaa
     1021 aggaatatat gctagcaaaa atgagatgct gatagttttg tttttactag tttctatttg
     1081 tagctcagtt attaatttag aaaatatagg attgattatc tgggggatac gaaataacac
     1141 tcgtatgttc atagtatata taaattgtgc aacatattta acaattaaag attactataa
     1201 agtattcgct ataattgaaa aattattctg gatttcactg ccactttgtt taattgaaag
     1261 attcatggtc acctatccaa ctggaacaat tgtaggagat atgataggtg gagttttttg
     1321 gaattattca ggttgtaata tgccattgaa tttaattttg tgtgtgacag ttactgttgt
     1381 tacaatcgga tatttcaagg gagaaaaaaa tttcatccat tttattttat gtgtttgtgc
     1441 agcacttttt atgtctgcat tagctgaatt gaaagttttt attcctgaat tatttataat
     1501 atttgttgca atagtattca ctttaaaagt tgattttaaa gtagtgatca agatattatc
     1561 atgcgtaata cttgtgtcga tagtattatc cttttttata acaatattta ttacattgaa
     1621 cgataatgga caaagctatg ctgataattt cacaattcaa ggtcttttag aatatgcgtc
     1681 aagagattct ggatatgatg gtgttggtga tttaaatcga ataactggta tctctagagt
     1741 agtagatgaa atatttaata ataattttag attagtccta atgggagtag gcattggcaa
     1801 tgcagagttt accaattttt ttgtcagtga gttttataaa tcgtttggta atcttcatta
     1861 tcagtatttt catcatattt ggttatttat agaagttgga atattaggag tttcgatatt
     1921 cctatttttt ataataaaga catttttcaa tgcaaaaaga tcttttatga aggataaaga
     1981 tgcaagtctt gttaaagtta ttgcaattat gtcggtattc ttttttgtgt ataacacaac
     2041 acttaggggt gaaacaacgg ggccactgat ttatatgatt ttagccatgc ctgaagttgt
     2101 gaaaaggaaa gagcattatt atagtgaaaa aaatacaaaa ggagagatat tatgattcct
     2161 aagaagatac attactgttg gttcggggga aatccacttc cgaaaaaaga tagaaagtgc
     2221 atagaaagct ggaaaaagta ttgccctgat tatgagatta ttgaatggaa tgaatcaaat
     2281 tatgatttaa ataagaaccg ttatatggcc gatgcatata aagaacgtaa atggggtttt
     2341 gttccagatt atgcaagatt tgatattatt tataatcatg gtggatttta tttagataca
     2401 gatgttgagc taattaagtc gttagatata cttcgtgaaa atcatgccta tatgggattt
     2461 gaaggggaaa tctgggttaa tggcggtatt ggttttggtg cagaagaagg acattctgtt
     2521 attaaaggtt taagggatat ttacgattca ttaagttttt atcaaaatga tggtacattg
     2581 aacttgaaac cgagtccgta ttatattaca gattatcttg aatctattgg attggttcgc
     2641 gataacagcg aacaaataat aaatggcctg catatctatc ctacggatta ttttgctgcc
     2701 aaggattatg atactggaaa aatttataaa actagtaata caataagcat tcatcaatat
     2761 aatgcatctt ggaaaacacc taaacaaaaa tttgtttata gattaaaatc aacaattgga
     2821 caaaaaaaat ttaacaggtt acttgcactg aaaaaatttt tacacttata aaggagaaaa
     2881 gtactttgaa gaaaatttgt ttcattagtg gtgtaatttc tcgttctggg ggaactgaaa
     2941 gagtagggag tattattgcc aatagcttgt ctgatttagg ttatgatgtc actattttaa
     3001 gcttttggaa tagtggagag ccttattttg ggttagataa gacgattaag gttgattata
     3061 tgttaaatcc taaaacggag ggaaaattat atagaactta tttatatccg gtttggaaat
     3121 tgcacaagta tatatgcaaa aatgaatttg atatagtaat tgatattgat actttgttgt
     3181 ctttgtatac tacaagggca attaaaggaa caaagtgcaa attgatttct tgggaacatt
     3241 ttaattactg gacaatggaa atgctgaaag aaaagaagag attcaaggct aaaaaattga
     3301 taaaaaaata tgcaaagagg cttgtggttc tcacagaaga agatcgtcaa aaacatattg
     3361 atgaatatag tttatcagaa gattttgtta taactatgcc aaatccgtgt atatctaata
     3421 tcgagtacga atatcattat gataaaaaga gatttcttgc agtagggcga ttagtggaag
     3481 agaaaaattt tgctgacatg ataagtgctt ggaaaattgt agaaaaaaaa gtagatgatt
     3541 gggatcttat cattgtagga aaaggtgaat tagaaaaaga tcttcgccag cagataaaag
     3601 atttaaatct gcaaagagta actatagcag ggcattcaga ccatgtggag gaatattata
     3661 aatctgcatc ttgttatgtg cttagttctg tttatgaagg atttcctatg gttatattag
     3721 aagcacagtc ttatggatta ccagttattt cttatgattg taaaactggt ccgagagatt
     3781 tggtacatca taattttaat gggatgttgg ttgaagataa gaatatagac caattagcta
     3841 agagcatgat aatgtttact aagaatactg atttagcaat gagaatgtca ctgaacgcat
     3901 ataataatgt acaaaaattt aatttgaaag aaattacaaa acaatgggtt gctttgattg
     3961 aaaatgtata gttttttgtg gttgtccaaa catgaaaatt agcgtaatta atcaaagagc
     4021 tagaagaaac ctattacatt tcaagatttt agaaattata tagtattagg taacaataaa
     4081 aaatagaaag tttggagcca tatgatatga taattaatcg taaacaaaat gctaaaagga
     4141 atataattac tggaacaatt tttaagttat atcagattat cattcccttt tttatgcgta
     4201 cacttatgat ttatcagatg ggaatggagt atgctggatt gaacaacctg ttcacatcta
     4261 tatttcaaat gttaaattta gcggaattgg gaataggtgc tgcactaact gtaagtatgt
     4321 ataaacctat agcagaagac aatagaaaag agatttgtgc tttactgaac gcatataaaa
     4381 agtacttttc aattattgga tggatagtgt ttacattagg attgttatgc attccttttt
     4441 tgagattttt agttaaaggt tcgattcctg gaaatttaaa tatgacagtt ttgtatttaa
     4501 tgtatttgat aaatactgtt ttgtcgtatt ggttattttc atatagaaag agtctaatat
     4561 atgcacatca aagaaatgat gtaattaacg tgtctatgct aattactttt acattccaat
     4621 ttgtaatgca ggcatatgtt ctgattttct taaaaaatta ctatttgtat ttattggctt
     4681 ctattttggg acaaatagtt cttaacttaa tgtgtgctgt tgtttctcaa aagatgtatc
     4741 ctgattatac tcctgttggt gagttagaga aagaaactat tgacgatatt ataaaaaaag
     4801 taaaaggatt agttacaaac aaggtaggcg gtacaatatt acgatcttca gattcaatta
     4861 ttatttcatc tttcttaggt ct
//
LOCUS       DAJSNV010000138         4785 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSNV010000138 DAJSNV010000000
VERSION     DAJSNV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.142"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(117..1079)
                     /locus_tag="ON516_002628"
     CDS             complement(117..1079)
                     /locus_tag="ON516_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3346072.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="ON516_002629"
     CDS             complement(1081..2520)
                     /locus_tag="ON516_002629"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3346073.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="ON516_002630"
     CDS             2705..4651
                     /locus_tag="ON516_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR3346074.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1528 a          810 c          780 g         1667 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaa
//
LOCUS       DAJSNV010000139         4681 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSNV010000139 DAJSNV010000000
VERSION     DAJSNV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.143"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            59..913
                     /locus_tag="ON516_002631"
     CDS             59..913
                     /locus_tag="ON516_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3346075.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ON516_002632"
     CDS             1010..3259
                     /locus_tag="ON516_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3346076.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..4437
                     /locus_tag="ON516_002633"
     CDS             3262..4437
                     /locus_tag="ON516_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3346077.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4440..>4681)
                     /locus_tag="ON516_002634"
     CDS             complement(4440..>4681)
                     /locus_tag="ON516_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3346078.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1633 a          674 c          850 g         1524 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagtaa cccgtggtgc tagttaattt
     4441 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     4501 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     4561 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     4621 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtggcgga
     4681 a
//
LOCUS       DAJSNV010000140         4439 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSNV010000140 DAJSNV010000000
VERSION     DAJSNV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.144"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            533..1444
                     /locus_tag="ON516_002635"
     CDS             533..1444
                     /locus_tag="ON516_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR3346079.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1931..2197
                     /locus_tag="ON516_002636"
     CDS             1931..2197
                     /locus_tag="ON516_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346080.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2269..2610
                     /locus_tag="ON516_002637"
     CDS             2269..2610
                     /locus_tag="ON516_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346081.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2665..3084
                     /gene="ssb"
                     /locus_tag="ON516_002638"
     CDS             2665..3084
                     /gene="ssb"
                     /locus_tag="ON516_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3346082.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3161..3457
                     /locus_tag="ON516_002639"
     CDS             3161..3457
                     /locus_tag="ON516_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346083.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3472..3903
                     /locus_tag="ON516_002640"
     CDS             3472..3903
                     /locus_tag="ON516_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3346084.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4085..4390
                     /locus_tag="ON516_002641"
     CDS             4085..4390
                     /locus_tag="ON516_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346085.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1629 a          698 c          952 g         1160 t
ORIGIN      
        1 tggtcaaaaa cctaagcggg aagtctccga ataacatacc aagcgccgac agccaacaga
       61 taaagaatac actactaaaa atagtagtag cataaaattt aaaagggaaa aaatcaaccc
      121 ctaccccagt cttggggtct caaaactaac tcccccctaa ccccctcttt cagcctcttt
      181 tgaaagaggg ggagaatcaa ccccacggcc aacgccccag tgttcttggg gctgtccaga
      241 acagaaactt gcgagagagt gggggtaaaa caagtgtaga ggaaacttga agacttgttc
      301 gaacagaaac ttgcgagaga gccggagtaa aacaagtgta gaggaaactt gaagacttgt
      361 tcgaacagaa acttgcgaga gagttggagt aaaacaagtg tagaggaaac ttgaagactt
      421 gttcgaacac aaacttacaa gagaggggag taaagcagtt gcagaggaat caggaagatt
      481 tgttcgaaca gaaacttgca agaaagcatt attaaattga aggtgatgaa atatgaagta
      541 tttattgcat ggctatttta ttgattattc tactcgcaat agtagtactt tagcgattct
      601 atgtttgctt catgaattga gagtggttac tagaaaacag ctatacaaac tattgaattt
      661 tgacagatca attagtaaac aaggagtaac gaatgcttta gtgagattat atgaaaaaca
      721 gttaatcgat cgaaaaaaat atggaacaga aatgtattat tatttgacga aagagggaca
      781 tcaaagcata ggtgggtatt atacgttgcc gaaagttcct gaatataatt tgaatcacca
      841 tttacaaatt aacaattatt tgattaaagg aatggcattg gcaaaagacc acccacattt
      901 aaaacggatt atttcagaaa gacgtaccgt ctatgaaacg aaggatttaa atcctaagac
      961 aaaaggaaga aaattttttg tgccagattt tatttttcgt tttcgagaca aagaaaatca
     1021 tgagatcaat tggtcctttg aaattgagct aacgttgaaa acacgtcgtc gctaccgtga
     1081 agcgattttt ccgaaatata ttcgtgagtt gcgaacacgt cctgaatcac atttaattta
     1141 tgtgacacct agtggcgcaa ttcaagaaga acttcatgcg tttaagcgta tttttttcaa
     1201 gcgcgaaact gaaaaagatg cgagtcaaca agaattgttt gagcggttgc atattttttc
     1261 agctttagag tttgaagagg gattaaaagc attattagag aatgatccag cagtcaattg
     1321 gcggacagta gaacgagaaa aaatttatcc gaatggcgct gttcaaagtc gtttgaattt
     1381 atttgaagaa tcaactgaaa atgaggaaaa accaaaagtg aagtttacgc ctgaagtatt
     1441 gtaaaagagg gtgttcgctt cgctcttttt ttgtgccagt ttgctggcac cgttcagacg
     1501 caagcgtctg aaccggctcg tttttaggaa ggactaactt gtttgattta tccgttgtca
     1561 aagaagtgga cgttcgagag tccctaatcg tatagcagtc attcggcgag tataacctaa
     1621 aacataacct gccaattgcc aagggtaaaa tgcacgctgc gcgcccttgg caatcgtcag
     1681 aacatgtttt cgagccggtt atgccgaacg accgctatac gaaaagggcc tctaaacaga
     1741 agtccacttt gatccttgac aactgaaacc caaaatcaaa caagcaggcc ttcgtctaaa
     1801 aacgagccga atgaagacct tgggggttgc acccccaaac ccccgtgccc ccgacacact
     1861 accgaaaagg tgggggtggg tggcgaaaaa atcaaataaa acattctatt caagaaagga
     1921 agaaacaacc atgacaacta aaaacgagca actgaaagaa caggcaagaa aagagttgca
     1981 agaaagaggt ttgattattg aggggagctt tgaaggagat tttgaaacct atattggttg
     2041 ttatgcaaga ccgataaaca aacctacagc cttagaccca atgaacgaac aagaagtaaa
     2101 agaacaagaa aaacatgcag tgaatggttt tccgcaagat ttcactgaat ggtatgagtg
     2161 ggaaattaac aacgggaagc ttgaaaactt cttataaaag tcagatgacg tgcaaagaga
     2221 atgattctct ttgtacggta aaaatataaa gaaagaagga aaaagaaaat ggcaaaaaac
     2281 acaccttatc aagtaggaga cattttttat agtagttggg gctacgaaca aacgaacgtg
     2341 actttttggc aaattgtgaa gttaacagaa aaaacagcat ggtttcagcc tattcaaaag
     2401 aaaacggtcg agcattattc gtccatgagt ggaaaagttg ttccaatgaa agacaagttt
     2461 atagagtatc ctttagcaaa aactaaagac agtattgtaa aaaaacgaat tgatccagaa
     2521 acctatcctg atatgttatg tggttatcgt agttgggaca cgtttcatag atataaaaat
     2581 caagctgttt atgaaagtag ttattattaa ggggatagtt tcccctttca aaaataataa
     2641 atagaagaat ggagagaaaa aatcatgatg aattcagtga atttagtagg acggttaaca
     2701 aaagaagtgg atttaaaata cacaccaaaa ggcaatgcga caggcacatt tattttagct
     2761 gttaatcgaa attatacgaa tgcaaacggc gagcgtgaag cagattatat ccgctgtgtc
     2821 atttggcgga aagctgcgga aactttagca aaatatactg gaaaaggaag tttgattggc
     2881 attaatggac gtcttcaaac gaggagctat cagaatcagc aaggacagac catgtatgtt
     2941 acggaagttc tagtgacaga tttctatttg ttagagagta aagaaatcaa tgagcaacgg
     3001 tcaaaatcaa tggcgaataa tcaaaataat tcttcaattc caggaaatga gattcaaatt
     3061 tcagatagtt atttgccctt ttaagtgaaa aataattttg ttctatacct gatttcttca
     3121 ggtatagaac aaaaaataaa tagagatgag gcgatttttt atgaaaatca ttgacagtta
     3181 cgatcaacga accatgcaat atttgggctc gtttgattat acagatgaaa atattattaa
     3241 ttatgttgca agtttacctc cttatcaaag cgttcgcttg gtagattttg caagtgatga
     3301 attggtatta acaacgatgg gcaacttttt agattatgtc ggagacaaaa aatggttgga
     3361 aaaaattttg tctaaattga ttgcgaaaca aacaggagaa gaacccataa atgcgattca
     3421 attgttcaat cgatatgaac aagaaaacaa tgattaaaga ggagagaaaa aatgaaaaga
     3481 ttaaaacgaa gtatttttat tgtgctagca ttgattgatt ttgttgggac aggttgtgaa
     3541 gcaattgacg ttcaaaaagc tcccttatcg gaaataaaac aagcaataac aaaacaagat
     3601 gaagaacaac cgctaaatac acaagtagaa atagtgaatc aaaataaacc gagtttttct
     3661 gaagaagatt tttcgttaga aaatggaacc aggcaatttt tttcagattt ggatcgttta
     3721 aatcgggtag gtgtagcaag tgcacttttg cataaaagca ggatgccaac agaagaacga
     3781 gaccgattat acattaagcc aactggctgg aaacaaaaga aaatgaagaa tggcgattat
     3841 ctttataatc gttgtcattt gattggctat caacgtgcga caagtttccc cataagtatg
     3901 taatacgaaa cgggaggatt aatcttaaga ttaatcacaa ctaacctatt tgacagttgg
     3961 aatgaaagct atcatgtctg ctgaatcaac tggtctgata ctcctacatg caataaagtc
     4021 tactaggaag ttattgtatg aagctaggta aaggcggcag aacaatttct aagtgtggaa
     4081 tcatatgaag ttggatatgc cgggacgcta taaattactc agggtgagac tgtttttaat
     4141 taaactgacg aacttgcgaa tgtacgggtc tataaccgac ttatattcga aagatataag
     4201 attgcgaatg caaagaacat tatcgaaaag taagaatgtt ggtcatgaaa ttcgagatgg
     4261 aagactagcg gtgtatctgt caagagttgg taaaacatgt ttgattgatt cagatgagag
     4321 tgggaatgcg acaaaacgtt ttacagaaga aatagagcaa ctagcagaac tgaatatacg
     4381 atatttttaa atactttagc ggagaataaa aaaatcctct acagaaaact acggagggc
//
LOCUS       DAJSNV010000141         4358 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSNV010000141 DAJSNV010000000
VERSION     DAJSNV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.145"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            414..971
                     /locus_tag="ON516_002642"
                     /pseudo
     CDS             414..971
                     /locus_tag="ON516_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1029..3068
                     /locus_tag="ON516_002643"
     CDS             1029..3068
                     /locus_tag="ON516_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3346086.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3177..4328)
                     /locus_tag="ON516_002644"
     CDS             complement(3177..4328)
                     /locus_tag="ON516_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3346087.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1571 a          802 c          856 g         1129 t
ORIGIN      
        1 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
       61 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
      121 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
      181 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      241 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      301 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      361 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
      421 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      481 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      541 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      601 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      661 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      721 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      781 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      841 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      901 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      961 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
     1021 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
     1081 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
     1141 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
     1201 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1261 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1321 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1381 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1441 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1501 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1561 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1621 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1681 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1741 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1801 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1861 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1921 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1981 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     2041 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
     2101 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     2161 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     2221 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2281 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
     2341 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2401 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2461 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2521 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2581 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2641 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
     2701 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2761 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2821 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2881 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2941 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     3001 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     3061 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     3121 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     3181 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3241 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3301 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3361 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3421 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3481 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3541 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3601 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3661 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3721 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3781 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3841 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3901 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3961 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     4021 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     4081 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     4141 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     4201 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4261 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4321 aattccattc tcatcattcc tttgtataaa atatagcc
//
LOCUS       DAJSNV010000142         4282 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSNV010000142 DAJSNV010000000
VERSION     DAJSNV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.146"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..1257)
                     /locus_tag="ON516_002645"
     CDS             complement(37..1257)
                     /locus_tag="ON516_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR3346088.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ON516_002646"
     CDS             complement(1277..1888)
                     /locus_tag="ON516_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3346089.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ON516_002647"
     CDS             complement(1931..2863)
                     /locus_tag="ON516_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3346090.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ON516_002648"
     CDS             complement(3284..4144)
                     /locus_tag="ON516_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346091.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAJSNV010000143         4232 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSNV010000143 DAJSNV010000000
VERSION     DAJSNV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.147"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(112..1878)
                     /locus_tag="ON516_002649"
     CDS             complement(112..1878)
                     /locus_tag="ON516_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3346092.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1838..3592)
                     /locus_tag="ON516_002650"
     CDS             complement(1838..3592)
                     /locus_tag="ON516_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3346093.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3702..>4232)
                     /locus_tag="ON516_002651"
     CDS             complement(3702..>4232)
                     /locus_tag="ON516_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3346094.1"
                     /translation="VISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNY
                     PRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWA
                     LMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLS
                     MIGVLIGRKVLKKYKK"
BASE COUNT         1429 a          801 c          596 g         1406 t
ORIGIN      
        1 actgattctt tcgtgtattc aaaattagat gctgtttaat aaaattattc tttttgaaaa
       61 agaattataa ctatatccca aaaattgtag gttaatttaa agcctatttc atcatcgtga
      121 ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta tgagattttt
      181 gtatatttct gaccttcgat acaactcatt atgagtacct tgatcaacta gttttccttg
      241 atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg atataattag
      301 tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt cattttccgc
      361 atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca aaaaagcccg
      421 tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa
      481 tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta aacgagctgc
      541 ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt ccaaaatact
      601 tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg
      661 agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat aatcatacag
      721 tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta ctaacgcagt
      781 cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat tgttatcata
      841 tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat ctgatccctc
      901 ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta aataaaatat
      961 ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg cggctagtaa
     1021 gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa cacctactag
     1081 aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga ctgaaaaagt
     1141 acttaattct acctttaaat gaatcctttc actctcttcc atttttttga aaagtctgtc
     1201 ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta tagtttcttg
     1261 aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt tcgtttggga
     1321 tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa cagctaatcc
     1381 cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca gagccatacc
     1441 gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt cagacatgat
     1501 agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta aatttgacaa
     1561 atcgtctgca atttgcactc ttaaatgcgc actttcttta taagtactat tatataagtt
     1621 ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacaaacg atacggcaaa
     1681 ataaagccaa cggggctttg tattttctaa aactaattcg tctatcagca tcattataat
     1741 gaatgctggt accatattaa tgcagtatac aaaaaaacta gaaatagaag ctttagttaa
     1801 agcttctgcg ccctgatcac ttaaagcaaa tttgttttta taaaaacttt tcattgtcaa
     1861 gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact tgatgtttag
     1921 ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag acaagaattt
     1981 catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt ttttctttca
     2041 tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata gatgcactgg
     2101 cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata gctatacgtt
     2161 gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc tctctttcgg
     2221 gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg
     2281 ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg aatagtttcg
     2341 tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag tgatttaaag
     2401 gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa attagtttag
     2461 ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt ttttgttcta
     2521 aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg acattttcaa
     2581 atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg tcttgcttca
     2641 tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac aagtgcatac
     2701 ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa aacaacatca
     2761 acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag gttataggaa
     2821 ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc
     2881 cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta ttcaaggctt
     2941 taaatgatgt tactttagca ccaaatatct ttataacttg aattccgcgg atatattcta
     3001 ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa tttgtattct
     3061 ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga accaatccaa
     3121 ttttaaagtt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact atggcttgag
     3181 cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg tcatcaattg
     3241 tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct tctgttaaac
     3301 cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata tgagaaaata
     3361 agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt tgaaccgcat
     3421 aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat ttgtaaatac
     3481 tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc
     3541 cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc attttttcct
     3601 cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc cgtaatagcg
     3661 taaattagag atgtacaaac agataaaagg taaactacgt tctatttttt gtattttttt
     3721 aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac taacactgaa
     3781 atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc agcagattgt
     3841 cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg aatccactgt
     3901 ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat tgctcgatca
     3961 tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga actgataaac
     4021 caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat tgtgtaaata
     4081 gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat tccagataat
     4141 aatccaaaaa tcccggggga tatgctgtga ccaaacgccc caccaatact actaaccatt
     4201 attgaaaaaa agtaaattac tagggaaata ac
//
LOCUS       DAJSNV010000144         4201 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSNV010000144 DAJSNV010000000
VERSION     DAJSNV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.148"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            69..770
                     /locus_tag="ON516_002652"
     CDS             69..770
                     /locus_tag="ON516_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3346095.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="ON516_002653"
     CDS             complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="ON516_002653"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3346096.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="ON516_002654"
     CDS             complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="ON516_002654"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3346097.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="ON516_002655"
     CDS             complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="ON516_002655"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3346098.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3352..4074)
                     /gene="tsaB"
                     /locus_tag="ON516_002656"
     CDS             complement(3352..4074)
                     /gene="tsaB"
                     /locus_tag="ON516_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3346099.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1245 a          892 c          728 g         1336 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg actgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata tttatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactgggc aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgatagat taactagagg aagcgtcttg
      961 tcctcatccg acctagatta ttcgatattc ctcgacaata aacgattaat cagtgagggc
     1021 aatcagagga acggataatg atgaataagc tgtaaaaaat agggctttga caaaagcgtg
     1081 tcacagccct tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata
     1141 atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg
     1201 acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa
     1261 cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag
     1321 ggcagatttt tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg
     1381 atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc
     1441 attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc
     1501 caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctacaat
     1561 cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat
     1621 cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg
     1681 tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt
     1741 atcttcatgg atcatcgctc gtggaaaatg gtaattatct tttccctgat gagctagctg
     1801 atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc
     1861 ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat
     1921 atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg
     1981 tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa
     2041 ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag
     2101 tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac
     2161 cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg
     2221 aactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat
     2281 ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag
     2341 aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca
     2401 tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat
     2461 tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa
     2521 tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt
     2581 tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca
     2641 ataaagccac agatctggtt atcttcaact aaaagcagat aatcagagtg aggctgttgg
     2701 atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta
     2761 atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac
     2821 taatctcact cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg
     2881 taatatcttt ttttgatcgt tgttgcttga aaacctaact tccgataaag tctttgtgca
     2941 tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag
     3001 ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg
     3061 gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt
     3121 cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata
     3181 aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc
     3241 tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgatt
     3301 gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt
     3361 ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac
     3421 ccgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcag ctaaagcacc
     3481 taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc
     3541 actaggcaat atttccttta tttcatagaa gaatttgtga caatcactcc cgacgaaata
     3601 aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc
     3661 ttcaagtaca atttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt
     3721 tcttcgagca tcaaacatgg gcacaataat tttatccaca ccgatgcaat tagccgcgat
     3781 ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc
     3841 tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg
     3901 gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag
     3961 tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc
     4021 taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt
     4081 gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat
     4141 gaaaatacca aaggcaaaac tagtacaatg atacatgatt atctgctggc gggcaaattt
     4201 t
//
LOCUS       DAJSNV010000145         4157 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSNV010000145 DAJSNV010000000
VERSION     DAJSNV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.149"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            179..775
                     /locus_tag="ON516_002657"
     CDS             179..775
                     /locus_tag="ON516_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3346100.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ON516_002658"
     CDS             899..2698
                     /locus_tag="ON516_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3346101.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ON516_002659"
     CDS             complement(2976..3188)
                     /locus_tag="ON516_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346102.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="ON516_002660"
     CDS             3650..3799
                     /locus_tag="ON516_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346103.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4052..>4157)
                     /locus_tag="ON516_002661"
     CDS             complement(4052..>4157)
                     /locus_tag="ON516_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3346104.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1494 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DAJSNV010000146         4107 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSNV010000146 DAJSNV010000000
VERSION     DAJSNV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.150"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..282)
                     /locus_tag="ON516_002662"
     CDS             complement(<1..282)
                     /locus_tag="ON516_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3346105.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMI"
     gene            complement(412..996)
                     /locus_tag="ON516_002663"
     CDS             complement(412..996)
                     /locus_tag="ON516_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3346106.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1327..1560)
                     /locus_tag="ON516_002664"
     CDS             complement(1327..1560)
                     /locus_tag="ON516_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3346107.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1575..2525)
                     /locus_tag="ON516_002665"
     CDS             complement(1575..2525)
                     /locus_tag="ON516_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3346108.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2522..3364)
                     /locus_tag="ON516_002666"
     CDS             complement(2522..3364)
                     /locus_tag="ON516_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3346109.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3366..4085)
                     /locus_tag="ON516_002667"
     CDS             complement(3366..4085)
                     /locus_tag="ON516_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3346110.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1392 a          758 c          495 g         1462 t
ORIGIN      
        1 gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt
       61 aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat
      121 caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat
      181 aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa
      241 aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa
      301 taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga
      361 tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag
      421 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
      481 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
      541 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
      601 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
      661 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
      721 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
      781 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
      841 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
      901 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
      961 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
     1021 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
     1081 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
     1141 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
     1201 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
     1261 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
     1321 agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa
     1381 ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga
     1441 tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt
     1501 tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat
     1561 acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc
     1621 atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt
     1681 gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc atccacactg
     1741 ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt
     1801 tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta
     1861 tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc
     1921 ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac
     1981 ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt
     2041 tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc
     2101 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
     2161 tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct
     2221 ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca
     2281 tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa
     2341 tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact
     2401 acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc
     2461 ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc
     2521 atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa
     2581 ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat
     2641 aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca
     2701 tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa
     2761 caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca
     2821 tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa
     2881 aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa
     2941 cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat
     3001 gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag
     3061 aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt tttataaaag
     3121 taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta
     3181 aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag
     3241 acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag
     3301 atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa
     3361 gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt taaattttct
     3421 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
     3481 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
     3541 tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct
     3601 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
     3661 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
     3721 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
     3781 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
     3841 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
     3901 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
     3961 gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
     4021 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
     4081 aacataactt tctccttcta aatgata
//
LOCUS       DAJSNV010000147         4090 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSNV010000147 DAJSNV010000000
VERSION     DAJSNV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.151"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..300
                     /locus_tag="ON516_002668"
     CDS             127..300
                     /locus_tag="ON516_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346111.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            940..1413
                     /locus_tag="ON516_002669"
     CDS             940..1413
                     /locus_tag="ON516_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3346112.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1422..1649
                     /locus_tag="ON516_002670"
     CDS             1422..1649
                     /locus_tag="ON516_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346113.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2285..2656
                     /locus_tag="ON516_002671"
     CDS             2285..2656
                     /locus_tag="ON516_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346114.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2912..3154)
                     /locus_tag="ON516_002672"
     CDS             complement(2912..3154)
                     /locus_tag="ON516_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3346115.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3186..4088)
                     /locus_tag="ON516_002673"
     CDS             complement(3186..4088)
                     /locus_tag="ON516_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3346116.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
BASE COUNT         1186 a          723 c          636 g         1545 t
ORIGIN      
        1 aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt tatttttata
       61 ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa aaacgaggta
      121 tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta tttgattgta
      181 tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac tactacatta
      241 tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg aaaaaaataa
      301 acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt tattcggcaa
      361 tccattttat ttttctactt cttcttaata cctctaacac ttttgattta taaactaatc
      421 caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc ccatgcgtca
      481 atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat aagtggtgat
      541 tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa cttgaaaaaa
      601 ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc tgttaactta
      661 cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata taatcccagg
      721 catctaaact agcattatag aagagacaca catagaatct aaaataactg gttttaaatg
      781 tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc tatcattttt
      841 tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca ataattattc
      901 tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta
      961 ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat
     1021 actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt
     1081 tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta
     1141 ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa
     1201 atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg
     1261 aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc
     1321 attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt
     1381 ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt
     1441 tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca
     1501 ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca
     1561 ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa
     1621 atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat
     1681 aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt
     1741 ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg
     1801 ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg
     1861 tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga
     1921 gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa
     1981 aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag taagctccca
     2041 tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt tactatgtag
     2101 ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt taacattatt
     2161 ttgataataa tgattattta tttaaaaata cattagataa catttgttac gacacgaaaa
     2221 agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa ttaggaggaa
     2281 tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata ttccattacc
     2341 aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg ctgctcttac
     2401 gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt attggctaaa
     2461 acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc tctttgagtt
     2521 tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga tcatctgggt
     2581 tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa aatttaggga
     2641 ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa ggaaaaactt
     2701 ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa agtcttgtca
     2761 cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa tatgcccaac
     2821 caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgaagaaca
     2881 gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag aatgatacga
     2941 ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct gcaatacttg
     3001 gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct gctacgacat
     3061 ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt gctccgataa
     3121 ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa tgttttactt
     3181 ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc gcttccagct
     3241 gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt cgctgcgtca
     3301 gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt tttgatgtcc
     3361 actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac ttctttgccg
     3421 attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc cacaatgaca
     3481 tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc cgttacatta
     3541 tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc agataataaa
     3601 ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc ttcataattc
     3661 agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt atttgtatga
     3721 tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc ttgtgctttc
     3781 gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat tgcatcatgt
     3841 tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt ttctgcatca
     3901 tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg atgtgcagat
     3961 actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg tgtcgttggg
     4021 tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt tttgttattg
     4081 ttattcatgg
//
LOCUS       DAJSNV010000148         4049 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSNV010000148 DAJSNV010000000
VERSION     DAJSNV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.152"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..297
                     /locus_tag="ON516_002674"
     CDS             64..297
                     /locus_tag="ON516_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346117.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(456..578)
                     /locus_tag="ON516_002675"
     CDS             complement(456..578)
                     /locus_tag="ON516_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3346118.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            768..992
                     /locus_tag="ON516_002676"
     CDS             768..992
                     /locus_tag="ON516_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346119.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1435..1641
                     /locus_tag="ON516_002677"
     CDS             1435..1641
                     /locus_tag="ON516_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3346120.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1641..1892
                     /locus_tag="ON516_002678"
     CDS             1641..1892
                     /locus_tag="ON516_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346121.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            1908..2063
                     /locus_tag="ON516_002679"
     CDS             1908..2063
                     /locus_tag="ON516_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346122.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            2073..2345
                     /locus_tag="ON516_002680"
     CDS             2073..2345
                     /locus_tag="ON516_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346123.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2790..2969
                     /locus_tag="ON516_002681"
     CDS             2790..2969
                     /locus_tag="ON516_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346124.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3028..3384
                     /locus_tag="ON516_002682"
     CDS             3028..3384
                     /locus_tag="ON516_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346125.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1436 a          641 c          783 g         1189 t
ORIGIN      
        1 tgatgatcta ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aattagtctg
       61 acgatggatc tattgatacc cgtaattgca tttatagacg ggatttcttt gggcgtcact
      121 gaaaatcctt tggtaaatag aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt
      181 gtctgttctt atcttttaaa aagagcagga aaggataatt ccttttggcg attgactaaa
      241 gtcacagtag cagcgacagc tgttatactt acggctattc taccgcttta tatgtagagt
      301 taacgggaaa tcgttgaact tcttgcgaaa tattgctata atgaacatga aaaagaggag
      361 ttgcgctaac aacccctcat taacagcacc gattaaagaa cggtggcaga atattagtta
      421 tttggaaata accgctgacc tgctaaagta gacggttatt tctttttgtc atttttaagc
      481 atatttacaa tcaaactaat caaagcaatg gtaaacatac caaaaccaag aatggtttga
      541 attgtctcat atgcggacaa aaggcttact cctttcatag attttagcac ttacattcat
      601 aagcaccacc acctttccag aagtcgccac cgtcttcaaa ctttactgtc ttgactatta
      661 tagcatatct aaaatcgaat aaaaattctc aattttagct gtttttgcta taattttagc
      721 gaggtgatcg tgatggaaca atatattggt ttactcgata aattagggtg atcaagacac
      781 agccattgct tgtgcgattt acattacgca cgagtcatga accaatcaat tgtgtagtgg
      841 ccaatataga aatagcggat aaattgctga ttttggaaga aggcaagtat actatttcag
      901 tgacaggtca cttcaataaa cgaaagcaac tagtcattga atcaatgcaa attagaaacc
      961 ctgatcattt tacgcgatcg atgagcatat aaaagaagag ctgcagtcct aaatgaggat
     1021 tgtagctctt tttgttttac atagaaacag taaaaatgaa aaggcctgtt taggaaggca
     1081 caagcgcctt ctcaattgaa agacctttct ctcactccaa ccgccaccgc cgttcgagaa
     1141 gaaaggaagt gaacgctacg cgttcactaa gataaagggt cttgcatagc aagacaaaat
     1201 agaaaaaatt tcctctcacc aaaaggttcg ctcctgttcg ttatctcttt attgatttgt
     1261 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
     1321 cgctgcgcgc ccttgtcttg tcgcttttcg ttgattcatt ctggccaaca aatcaataaa
     1381 gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc aatcatgcga
     1441 aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta tcatgcacca
     1501 catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta ttgtttgtat
     1561 caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattatat tttaattgat
     1621 gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta aaagaagtcg
     1681 ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagtt ttacttattc
     1741 cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg ctatttgaag
     1801 acctagtgaa gattgcagga tatgaactgc attatcaaga aaacttggaa acttatgaaa
     1861 acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg gctcaaaatt
     1921 actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga
     1981 cgttcctata caacgaaaca cgtgttccaa aaaaacatta taaagaaaaa ttgtcggtag
     2041 ctgtggaaga aatgattgaa taaggtgtcg aaatgaatct tattgcgacg tactatcgaa
     2101 cactggaaga actgaaaaag caaaatgcaa aatggttttt ccaagcctta ctatgcttgg
     2161 aagtaggtgt caaaccaagc actattaaac cttctgaata ccaagcttta gaactaacgt
     2221 atgcgaaatt tatcgaaacg aaaaaagcca agactgtttc aagtgaatgg ttagattatt
     2281 ttgaaaatat caataagtat ggcgcatatt acaccatgaa aaaggaagac aacgaaaatg
     2341 aataaggtga tttatcaact aacaacggat tccatatcaa cgatttgaaa gaaaaaaagg
     2401 tcaataatca tttataccga aaagtgagaa tgtgtgaaac aaggacaaaa aaagcctagt
     2461 aactaaggaa atggttctta tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg
     2521 gtgcgaaata aacgggcaaa gcccgataac ttaagagaaa gaaggtgatc catttgaaca
     2581 agattattcc cttattgtta gtactgtttg ttggcgaaat acttatgcga gatagtgcag
     2641 ctgtacagtt aaacctcgct gtatttttgg taatactggt gttttgtttg atcttttggc
     2701 ggtttgtatt agtcctcgtg agtctactat tcaagatgat tgcagccata tttttcatag
     2761 ggttatttcg atgaaaggag tgagtaccaa tgccaattgc aataggaaat aaacggttac
     2821 ccgtgacatt agatgaaaag agacaaaaag aattgcagca actaaagcag aagtatggca
     2881 aaagtgaatc taagattatg tgtattgcgt tagatctatt gatcgcgcaa gaaaaagcag
     2941 gatttgatgt accagcgcta aaaaagtgac gtcacttttt aatttgatct atataagtga
     3001 tatcactttt accaaggagg gaattaaatg ttaaaaacta aaaagaattc gtacaaatta
     3061 aagcttagta tactatctat tatttgtctt ttaggcatga taattagtgg tcatttgtat
     3121 agagtggcat atacgcagtt tattgagtac aaggttcact ctttaggatt agctggggat
     3181 cgatacgttg aatatgtcca ttattcatta aaatgttctt catatattct tctttggctt
     3241 attttttcat tgtttgaatt gctaaattta atagcaatat cttcatatca cttagataag
     3301 ctaaatacca aatctagccg ttataacgaa gtgtttaagg aaaatgtgaa gttagaaagt
     3361 gacttaaaca agctaaaaaa ataaaaagaa gaaaacataa gttaactaaa attattttat
     3421 taagaatgat ggaatataag gataaggtta ccaacataac gatcattact tctctgatat
     3481 gctatggact tctatcaatc agtattttgt ctatagatta tggagtaact atactgcaag
     3541 ccttagtttt ggctatgcct atgtatctat ctttgtttat catgcatgaa atatataagc
     3601 tatttgtttt atcaaaaaat gtagaaatac gggtaaatat tttggtgaat agttatcaaa
     3661 aagaactcaa agaaagaaga cggtggcggt atttattgaa aggatgaaaa agtaggattt
     3721 gacatactgg tattcagaaa gtgccgtcac ttttaaaaca atggaaaagg tggagataag
     3781 atgaagaaaa aacaaactaa actatcatta gcagatattt aacacagtta accgcaactg
     3841 aagatggtgt tgttgaattt gaacaatctg aaattgcggt agtagatgat cgttacctta
     3901 aaatgcctta cttctttgac caagcaaagg tcatttgcct ttgtcactac gatggggtac
     3961 gtgattttag gcgttaaaaa gtagtgatta tatcaacttt acgagtaaat ataaatttag
     4021 acatgaaact gaattatcca atggaaaat
//
LOCUS       DAJSNV010000149         4001 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSNV010000149 DAJSNV010000000
VERSION     DAJSNV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.153"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(72..614)
                     /locus_tag="ON516_002683"
     CDS             complement(72..614)
                     /locus_tag="ON516_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3346126.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            749..1138
                     /locus_tag="ON516_002684"
     CDS             749..1138
                     /locus_tag="ON516_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346127.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1142..1906
                     /locus_tag="ON516_002685"
     CDS             1142..1906
                     /locus_tag="ON516_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3346128.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2035..2982
                     /locus_tag="ON516_002686"
     CDS             2035..2982
                     /locus_tag="ON516_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3346129.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3112..3852
                     /locus_tag="ON516_002687"
     CDS             3112..3852
                     /locus_tag="ON516_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346130.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1329 a          680 c          810 g         1182 t
ORIGIN      
        1 aaaataataa ataaaaactg tctaacattt aaaatttaat ttagatatta gacagttttt
       61 aagtttgtga gttattttcc ttgtcgtttt ctcacttttt tagtccagaa accacctgaa
      121 atatattttg gctcataaga aatgataaaa gcacgctctt cgatttcttt gatcaggcga
      181 tacagttcac gttcgctttt tctaggagaa agtatttcga gaactagccg ctcaccttcc
      241 agccccattg cagaagtctg tgtcacacca taaccgtgtt ctctcagtat acccggcaaa
      301 cttttatctt ctgcgctatt tgctggcaaa atcgttgtga ccatgatgta tcctaatgcc
      361 aatttgtctt caatcaaaat tccgacactg atacctactg cgtaacctaa cgcatagaca
      421 aataaattca gtggattatc taaacgatct aaaacaaggg ataagccgac gatataaatc
      481 gtgatctcga ccatactgac aagtggagca atcagtcgat agcctttcat tgtcaacatg
      541 aaacgtaacg tgttcagagt gatataagca aaattaatga ggaagatcat ccccaacatt
      601 tttggatcaa tcatttgtaa aactcctttc atttatataa caacaatatc aaaaaaaatc
      661 gtaagtgaag aaaaattgaa actttataca tacaaaaaaa tgataaaatg attataatag
      721 aattataaag ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt tttgtgccag
      781 ccacgtgcta agtctccttc tcagtatata cttaggtgtc tattcgattc aacagccaat
      841 tgaggtagag aacattgttt ttcagatgtc gctgacagat ggtttaattt taagcgtttt
      901 tttctttttg atactgttta ttggtaatat tttcggtagc gttgccagtt ttttgaattt
      961 ttctatttat ccactccttt cgctagcatt aggagtggtg ggactttgtt ctctgtgctt
     1021 atttcccgag ccattcagtc ctgcatttat attatttggt acactgaacc tgttccagac
     1081 aactgttgga gcatggttgc tatggcgatc aggaaattta atgaaaatag gtgagtaaca
     1141 attgaatcca attatattga aacgttctgt ccatcatatt ccagtgttag aagttgttct
     1201 gaaagaatta agagaggaac gtattcccgt tgtcatctat tatcacggat ggcagacaag
     1261 taaggagtta gttttgacgc aaggaagaaa aattgcgcag cagggcattc gtgtgatcct
     1321 gccagatgcg atgaatcatg gggagcgcaa acagccggtt tcttctattc catcctatac
     1381 attttgggat agtatctacg gaaatctgtt tgaatttgac acacttatca agcatttaga
     1441 aaaaagaggt cttctacatg atcagcttgc agttggcggg gtttcgatgg ggggaatgac
     1501 aacatgcgcc ttgttggcta aacatcctca tatagtagcg ggcgtgtgtt tgatgggttc
     1561 ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc gcatcggaat ataattatgt
     1621 cgtaccaaaa gactattttg atttgatttc atgggttaag agctatgatt tgtcattgca
     1681 gccggaaaag ttagctggac gtcctctgtt cttttggcat ggacaagctg acgaaaaaat
     1741 cccttatata caaacggaac gatttgtaga aaaaaatcgg cataagtctt acggaaaaga
     1801 aattgttttt cgttcctcaa aaacacagcg acatatggta caaataccac taatggaaga
     1861 agcttccact tttttagcaa aaaatttgat aaaaaagtat aaataattga aacggaaaat
     1921 cactcatgac gatagaggat tttccgtttt ttattttggg ctataataaa ctctgttagg
     1981 aaaatttggt ataattgata gtaagaatga caacatgtta gaaaggaaga gagcatgccc
     2041 actttagcag atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc tcgagtcatc
     2101 aatcatccgg aacaggtgac tgacgaattg aaagagttag ttttctcagc aatggctgaa
     2161 ctggattacc gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc gcagatcatc
     2221 aaattattta tactggaaga aatcgacaca actgagccct attacatgaa tttgctgatg
     2281 ggaatcgcta aacggatcgg taaagcgcat tattctctcc aattagttac aaatgatgcc
     2341 ttcgacgtcg gttcttgtga tggatacatc atcacaggtg tcagaaaaaa agacttcgat
     2401 tggatcgacc gtttggaaaa accagttgtc ttgtttgggg aaaattctga aggatatgat
     2461 tatgtagaca gcgataatga atatggtaca gcaaaagcaa cgcaatatgc cttagaaaga
     2521 gattatcaaa cgattattta tatcggtatc gaagtggacg agccttttga aatttcaagg
     2581 gaaactggtt atttatctgt gatgaaggaa gttgatcttc ctagtaaaat cttgcggttt
     2641 ccaaatcatt cgacagaagt agaaagatat atgacagaaa actgggcaga ttttacagat
     2701 aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag gagcagtaag agggattata
     2761 aatgctggag gacacttgcc cgaagaattt ggtgtgattg gatttgatgg ggtattttta
     2821 gaccaaatca gttcacctaa attgacaacg atcaagcaag atattatacg tatgggtgaa
     2881 gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca aggttatcgt
     2941 cgtttttttc ctgaactagt tatcagagag acaacgaatt aagtcttatt actatatata
     3001 tatttatgaa attcatcgct attttcttaa aataatcagc gcattgctac tgaaaacaat
     3061 gaatctgtgc tattattgag aaaagttcca tttttgagaa ggatggtttg aatgaaagaa
     3121 atcagacgaa aacatttccg ttttcctgcg tacgatgatg agttaggcgt aaaattaacc
     3181 aacgacaaga agcgagtatt atttgacgga caagaagatc ttttgactga acagaatcag
     3241 tccccgtttg aacagatgaa agaatttcat ttcacgaaca cgaacaccga gaaaatccgt
     3301 cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa tttggaaaag
     3361 cataaaggaa aattaccgga ttataccggc cattaccaat cagaaatcac atcgacagga
     3421 cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac aaagtgaaac aaggacagag
     3481 tccaagcata ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt
     3541 gttcctaaat atgtacccgc atcgattatc ccagacccta gagaaagcaa aatttctgag
     3601 gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt cgatacagaa
     3661 ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg tacgaacatt caatcgaaca
     3721 aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatc
     3781 ttagaacgct cattacaagg attgatcgat gatcaaggaa actctttggg agaaaatagc
     3841 tattttaaat aagtgatata caaatttata ttcggctaca gttggaaagg aaacgctgcg
     3901 gcgtttcctt attttatact ctatccgaaa gtttatatcg tatcctcaaa tttttttgag
     3961 tttaagcgaa ttttttctcc caataagtaa gaaaataatg g
//
LOCUS       DAJSNV010000150         3916 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSNV010000150 DAJSNV010000000
VERSION     DAJSNV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.154"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(25..1530)
                     /locus_tag="ON516_002688"
     CDS             complement(25..1530)
                     /locus_tag="ON516_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3346131.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="ON516_002689"
     CDS             complement(1527..2213)
                     /locus_tag="ON516_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3346132.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="ON516_002690"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="ON516_002690"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3346133.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DAJSNV010000151         3901 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSNV010000151 DAJSNV010000000
VERSION     DAJSNV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.155"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            326..640
                     /locus_tag="ON516_002691"
     CDS             326..640
                     /locus_tag="ON516_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3346134.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            656..1042
                     /locus_tag="ON516_002692"
     CDS             656..1042
                     /locus_tag="ON516_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3346135.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1071..2456
                     /locus_tag="ON516_002693"
     CDS             1071..2456
                     /locus_tag="ON516_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3346136.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2459..2611
                     /locus_tag="ON516_002694"
     CDS             2459..2611
                     /locus_tag="ON516_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3346137.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2634..3839
                     /locus_tag="ON516_002695"
     CDS             2634..3839
                     /locus_tag="ON516_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3346138.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1291 a          712 c          835 g         1063 t
ORIGIN      
        1 aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg tcaaaaccac
       61 tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa
      121 aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa
      181 aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa
      241 aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa
      301 taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa
      361 aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg
      421 acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga
      481 tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt
      541 gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac
      601 aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat
      661 gagattagca aatggcattg tattagataa agacacgact tttggagaat tgaaattctc
      721 tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa
      781 ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc
      841 tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat
      901 tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc
      961 agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa
     1021 agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc
     1081 gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac
     1141 tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca
     1201 actggcagga ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca
     1261 gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct
     1321 atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca
     1381 agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta
     1441 caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac
     1501 agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga
     1561 aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg
     1621 aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta
     1681 aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata
     1741 tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag
     1801 ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct gaccttgcgg
     1861 acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg
     1921 aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata
     1981 agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat
     2041 acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga
     2101 aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc
     2161 cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag
     2221 gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct
     2281 tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt
     2341 tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca
     2401 acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt
     2461 gctggctggt gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta
     2521 caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac
     2581 aaaatttcag cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg
     2641 gatttttact gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg
     2701 gactatctca aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata
     2761 ttgaaacagg aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg
     2821 aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc
     2881 cgacaacaga cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc
     2941 ttcatgagga ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg
     3001 tcctttgctc ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca
     3061 gacaatttga aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg
     3121 ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga
     3181 ttttgaatat ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc
     3241 gcagttttaa aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg
     3301 gaaacaccct ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg
     3361 actacgagca gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt
     3421 ttgagattcg attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg
     3481 acaatccaga gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag
     3541 acgattccaa acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga
     3601 acaatcgtga acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc
     3661 tgaactggct atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa
     3721 tcaaccagac gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca
     3781 agcagatttt gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac
     3841 tcaaatacaa attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc
     3901 t
//
LOCUS       DAJSNV010000152         3801 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSNV010000152 DAJSNV010000000
VERSION     DAJSNV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.157"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="ON516_002696"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="ON516_002696"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3346139.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="ON516_002697"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="ON516_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3346140.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="ON516_002698"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="ON516_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3346141.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="ON516_002699"
     CDS             2855..3730
                     /locus_tag="ON516_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3346142.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         1301 a          661 c          853 g          986 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta a
//
LOCUS       DAJSNV010000153         3738 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSNV010000153 DAJSNV010000000
VERSION     DAJSNV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.158"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(152..427)
                     /locus_tag="ON516_002700"
     CDS             complement(152..427)
                     /locus_tag="ON516_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR3346143.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="ON516_002701"
     CDS             complement(399..1352)
                     /locus_tag="ON516_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3346144.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="ON516_002702"
     CDS             1964..3457
                     /locus_tag="ON516_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3346145.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..>3738
                     /locus_tag="ON516_002703"
     CDS             3592..>3738
                     /locus_tag="ON516_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3346146.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVA"
BASE COUNT         1255 a          619 c          662 g         1202 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgct
//
LOCUS       DAJSNV010000154         3663 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSNV010000154 DAJSNV010000000
VERSION     DAJSNV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.159"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..276
                     /locus_tag="ON516_002704"
     CDS             <1..276
                     /locus_tag="ON516_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HCR3346147.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ON516_002705"
     CDS             419..640
                     /locus_tag="ON516_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346148.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ON516_002706"
     CDS             656..1498
                     /locus_tag="ON516_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346149.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ON516_002707"
     CDS             1561..3363
                     /locus_tag="ON516_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR3346150.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ON516_002708"
     CDS             complement(3422..>3663)
                     /locus_tag="ON516_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3346151.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAJSNV010000155         3613 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSNV010000155 DAJSNV010000000
VERSION     DAJSNV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.160"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            56..1045
                     /gene="galE"
                     /locus_tag="ON516_002709"
     CDS             56..1045
                     /gene="galE"
                     /locus_tag="ON516_002709"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3346152.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1057..2541
                     /gene="galT"
                     /locus_tag="ON516_002710"
     CDS             1057..2541
                     /gene="galT"
                     /locus_tag="ON516_002710"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3346153.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2565..3545
                     /locus_tag="ON516_002711"
     CDS             2565..3545
                     /locus_tag="ON516_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3346154.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1223 a          654 c          800 g          936 t
ORIGIN      
        1 tttaaattat atagaatcaa caaacacaat tttactaatc aaaggagtgt tttttatgac
       61 aattttagta ctaggcggag ccggttatat cggttcgcac gctgtagacc agctagttca
      121 aaaggggtat caagtagctg tggtagataa tttgctcaca ggtcacaaac aagcagtaca
      181 tccagatgct catttttatg aaggagacat ccgggataaa gaatttctac gttccgtatt
      241 tgaaaaggaa ccgattgaag gagtaatcca ttttgctgca agctctttag taggagaatc
      301 tgtcgaaaaa ccattgatgt atttcaacaa caacgtatac ggtatgcaaa tcttacttga
      361 agtcatgcac gagttcaacg tgaataaaat cgtattctct tctactgcag caacttatgg
      421 agagccgaaa gagtctccaa tcacagaaga tacaccagct aatcctaaaa atccgtatgg
      481 tgaaagtaaa ttgatgatgg aaaaaatgat gaagtggtgt gaccaagcat atggcatgcg
      541 ctatgtcgct ttgcgctact tcaatgtagc cggagcaaaa gcagatgcat ctatcggaga
      601 agatcataca ccagaaacgc atcttgtccc aatcatttta caagtagctc ttggtcaaag
      661 aaaagccttg gctgtttatg gagacgatta tgatacacca gatggaacat gtatcagaga
      721 ttatgttcaa gtagaagatt tgattgctgc acatatttta gctttggaat acttgaaaga
      781 aggaaatgaa agcaacttct tcaatttagg cagcagcaaa ggatactcag tcaaggaaat
      841 gctagaagca gctagagaag tgacaggaaa agaaattcca gcagaaatag caccaagaag
      901 agctggagat cctagccgat tagtcgcttc aagtgaaaaa gcgagagagg ttctgggttg
      961 gaaacctgaa tacacagata tcaaagcaat tatcaaaaca gcatgggatt ggcatgtcag
     1021 ccatccaaat ggttatgaag aataggggaa tgagatatgt caatcagtca aacgatttgt
     1081 gattttgcca cacttgcgat cgaagctggc ggatggatgg aaatggaccg cttatattta
     1141 cagaatcgtc ttttagggat ggtaggagaa gatgctctag aaagcattga agtaagacca
     1201 gttaaggaag aatctgtaga cttattggat cagcttgtcg ctaaggcaag agaaaatggt
     1261 gttctttcag aattgccagc agaacaagag atttttgaag cgcaattaat ggattttctc
     1321 acaccgccac cttcagtcgt caatgcattc tttgcacagc actattcgaa aagcccagag
     1381 gaagcaactg agtatttctt taatttaagt aagaaaaatg actacatcaa aacaagagcc
     1441 atcgcaaaaa acatcaaatt tccggcagag tcggagtatg gtgaactgga aatcacaatc
     1501 aatttatcta agccagaaaa agatccaaaa caaatcgcag cagaacgcga ggcggtaaaa
     1561 gtagactatc ctacctgcat gctttgcatg gaaaacgaag gatatagagg tcgcttgaac
     1621 tatccagccc gcaccaatca ccggattatt cgaatgaatc tagacggtga aagctgggga
     1681 ttccaatatt ctccttatgc ttattacaat gaacattcga tcattttatc agaacaacac
     1741 cgcccgatga aaatcacaaa ggaaacattt gctagactgc ttcggattac tgaagtcttg
     1801 cctcattact ttgttggttc gaatgctgac ttgccgattg ttggaggatc gattctttct
     1861 catgatcatt atcaggcagg acgtcatgaa tttcctatgg caaaagcacc gatagaaaaa
     1921 cttatcaaac tagcagatta tccgcaagtc atcgcaggag tcgtgaaatg gccgatgtct
     1981 gtgatccgtt tgcaatcaac agagaaaaaa ttgttgattg aagcagctaa ctcagtattg
     2041 gaaaagtgga aagcatattc ggatgaaaca gtatctatca gagctttttc agcagatggt
     2101 acaccacacc atacgattac gccaattgca cgaagaaggg acaattactt tgagttggac
     2161 cttgtccttc gagacaacaa tgtttcagaa gaacatcctg atgggatttt tcatccacat
     2221 aaagatgtcc aacatatcaa aaaggagaac attgggttga tcgaagtaat ggggcttgct
     2281 gttttaccac ctcgtttgaa accagaacta gtagaagtag agcatttctt attaggagaa
     2341 gaaaaccaga tggctgaata tcatcgtccg tgggcagaac aattaaaaaa agaacatcct
     2401 gatttgacga aagatacggt caccgatgtc gtacaaaaat ctgtaggtca ggtttttgca
     2461 cgtgtactgg aagacgctgg agtattcaag cgagatgaaa aaggacaggc agcgtttgaa
     2521 cgatttactg caattttata aacgtaaaaa aggaggagag acaaatggca acaattaaag
     2581 acattgcgca gttggcagga gtttctcctg ctactgtttc aagagttctc aactatgatc
     2641 ctgatctttc cgttggctta gccacaaagc aaaaaatttt tgaagcagca gaacgcttaa
     2701 attatacgaa acatcgtaaa aatgcagcaa atactaagca aacgcttcgc ctgatccagt
     2761 ggtacgacga tacggaagag cttgaagatc tttattattt atctattcgt ctaggaatcg
     2821 aaaaaaaggc agaagaagca aacgttactt tattgaaaga aacgtgggat tccattagtg
     2881 atcttccgac agatggaacg atcgcactag gaaaattcga cgaagctcag ttattagaaa
     2941 tgaaaggatc ccaaaatgcc ttgcttttgg tagattctga cggcacttct caaggaatcg
     3001 actctctagt tgtcgacttt aagtcaagtg ttcaaaaagt cattgaacat tttgtctccc
     3061 ataaagcgca acatatcgct atgcttgctg gaacagaata tacgaagaaa aaccatcagc
     3121 ggctaaaaga tcccagaatt atggcatttc aacaagtttt ggcagaaaaa ggctacgacg
     3181 aaggtccaat tatcgaagca gatttttctg tcgagtcagg atatcaagcg gtgaagcagt
     3241 atttaaaaac gaatccgcat gttcctgatg cgctttttgc agctaatgat gcattagcga
     3301 ttggcgcact gaaagcgatt caagaaaaag gtttagtggt accagaagat atttctgtga
     3361 tgggtttcaa tgatgtcagt gtagcgaaat atgtaacgcc ctctctttct actgtaaaag
     3421 tttataccga atggatggga gagctagcag tggagacgat tcttcaatta agtaattcca
     3481 aagcaccggt ttcaagaaag attattgtgg aaacagagtt gattattcga gaatcaacga
     3541 aataaaagat ggaaaagctg tggggaaatg aagtgatacc gcagcttttt tgtgtttcag
     3601 gaaggggcaa aat
//
LOCUS       DAJSNV010000156         3499 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSNV010000156 DAJSNV010000000
VERSION     DAJSNV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.161"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            960..1928
                     /locus_tag="ON516_002712"
     CDS             960..1928
                     /locus_tag="ON516_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3346155.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="ON516_002713"
     CDS             1945..2469
                     /locus_tag="ON516_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3346156.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2645..3049
                     /gene="tnpA"
                     /locus_tag="ON516_002714"
     CDS             2645..3049
                     /gene="tnpA"
                     /locus_tag="ON516_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR3346157.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            3066..>3499
                     /locus_tag="ON516_002715"
     CDS             3066..>3499
                     /locus_tag="ON516_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346158.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT         1317 a          587 c          605 g          990 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taacagaatt aggcagaagt cgcccatcct ctataggtgg
     2521 gtgatgaatg ccgttcggta tgagggttac cgttggtaac tcgaagaccg acaactaagg
     2581 cgtattgcct ttttattttg tcccatgtta caatcagtct atacaagaaa tgagttgata
     2641 taacatggaa ttagatacaa ataatcattc agtatttctt ctttattacc accttgtttt
     2701 agtaacgaaa tatcgtagac aagtgattga tgatgaaata tctgattatg ccaaaactac
     2761 ttttgaaaga atttcagaat cgtatcacat tactttagtg gagtggaatc atgataaaga
     2821 ccacgttcat attatgttca aagctcaacc taaaacagaa ttgacaaaat tcatcaatgc
     2881 ctataaaagt gccagctcaa gattgataaa acgtgacttt ccaagagtca aacaatttct
     2941 ttggaaagaa atgttctggt ctaaaagttt ttgtcttctg acaactggtg gcgcaccaat
     3001 tgatgtgatt aagaagtata tccaaaatca aggaaacaat cataaataga aagtaggtga
     3061 actttatgga acaactaaaa gcatacaaat tcagaatcta tccaacagag gaacaagaaa
     3121 tcttctttgc caaatctttt ggttgtgtcc gtaaggtcta caatctaatg cttgatgacc
     3181 gaaaaaaggc gtatgaagaa gttaaaaatg attcttctaa aaaaatgact tttccgacac
     3241 cagctaaata caagaaagaa tttccgtttt tgaaagaaat tgatagcctt gctttagcca
     3301 atgcccaact taatttagat aaagcataca agaatttttt tcgggataaa tccgttggat
     3361 ttccacgttt caaaagtaag aaaaatcctg ttcagagtta tacaacgaat aatcaaaatg
     3421 gtacagttgc tttgattgat agtaaattca tcaaagttcc taaattgaag tcattagtaa
     3481 gaattaagct tcatagaca
//
LOCUS       DAJSNV010000157         3475 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSNV010000157 DAJSNV010000000
VERSION     DAJSNV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.162"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..440
                     /locus_tag="ON516_002716"
     CDS             <1..440
                     /locus_tag="ON516_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraG family conjugation protein"
                     /protein_id="HCR3346159.1"
                     /translation="EINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            1153..3051
                     /gene="ltrA"
                     /locus_tag="ON516_002717"
     CDS             1153..3051
                     /gene="ltrA"
                     /locus_tag="ON516_002717"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3346160.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            3168..3475
                     /locus_tag="ON516_002718"
                     /pseudo
     CDS             3168..3475
                     /locus_tag="ON516_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT         1314 a          550 c          716 g          895 t
ORIGIN      
        1 gtgaaatcaa ttttgattgt catttagttg caggtgatca agtttgttta aaatacgatc
       61 atcaaggaaa agaatatgtg gcttggtatg aattttctca aagagtattg aaagacgaag
      121 agggtaatat cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag
      181 aagccgttct aacattagat gaggagaaaa gtaacttatt agtagagtct attcggatgc
      241 actataccga taaaccaatg gctattttta tgcttacacc tgattatgat ccatctaacc
      301 atatcattgt tagtattttt cttagtcagt tatataaaga attatctacg caatgtgtta
      361 agactaaagg agataaatgt caccgcagga ttcattttat tttagatgaa tttgggaata
      421 gtgtgacctg tttagcctaa ggataaaaga ggctagttcc aacgaacatt ggaataactt
      481 tactatcgag aaatcaaatg acggggtaac gccctgaagg gtttccactg atactgcgac
      541 atgcgttagt aggatgtgca tccaaacgcg tatgaagctc gctgaagtcg gcagaagggt
      601 accgtaagga aagacacgat acgtatctac cacgcaagtg ctctagaggt agggtatgta
      661 cctgatatgt cgggtgtcct aaaaagttca ataaggtgag aatgatttga ttatctgaca
      721 aacttgcgaa tggaatgtct gaaacgaata actcatagaa atgtgagggg gaagggggat
      781 acgtcctctt tccattgact cgaggttgag taaaaatgtg tttctatgaa aaaccttgca
      841 tggttaaagg ctatggagta gtcaacaggc aaaagcaagc acctaagttg aaatctatgg
      901 atagatagta aggaacaggg gaagcatcta gcacggaggg agcataccga agatgtgttt
      961 ctaaggaaaa accgaaatcg gttataactt aggtgaatag atgtgaaagt agagccacga
     1021 ccagtgaaaa tttcgtaatg aaattggagg aacaggctca agtcgttatt tataatataa
     1081 gaaacctatt taaaatcatg gattctcgta agactaaaaa ggtcacgaac cagaatggag
     1141 cgtgataaaa ccatgactaa cacaaaagga attagcttaa agaacaaaca actcagacac
     1201 gccgaatact acgacatgac tgagactttc gatgaattgc acgcaatgag tgttgcaaaa
     1261 tcaaatttta aaaatctcat gaaattcatt attgatgaca ataatatctt tctagcgtat
     1321 agaaatatca aaaagaataa aggaagtgca acggccggat acgatggtgt cactataaaa
     1381 gacattgaaa aattggcatg tggtgacttt ttaactatta ttaaaaggcg atttaaggat
     1441 tacagaccaa gaaaagttaa aagagtagag atcccgaaac caaacggaaa acttcgacct
     1501 ttaggaattc cttcaatatg ggacaggata gtccagcaat gtattctaca aattttagaa
     1561 ccaatatgcg aagcaaaatt taataagcat agctatggat tcagaccaaa ttgttctaca
     1621 gaaaacgcca tagcagattg tcttgctaga atcaatcaaa gtaagatgaa ttatgttgta
     1681 gatattgata taaaaggatt ctttgacgaa gtaaaccata ccaaacttat gcgtcaaata
     1741 tggacaatgg ggattcgaga taaacaactc cttgtaatta ttcgtaagat gttaaaggct
     1801 ccgattgtta tgcgaaatgg caatgttgtt tttccaacca agggtactcc acaaggaggg
     1861 attttaagtc cactgcttgc gaatatatgc ctgaatgaat ttgattggtg gatagctaat
     1921 caatgggagg aacgaactgt caaagagctg aaacaccact atcgaaaaaa cggagcatgg
     1981 gataaaggaa atatctattc gaaattaaga agccacacaa gtttaaaaga gatgtatctg
     2041 gtacgctatg cagatgattt taaaattttc tgccgaacag gagaagaagc ccaaagggct
     2101 ttttatgcca cacaaaaatg gctcagagaa agattaaagc tcccaatttc aatggagaaa
     2161 tctaaagtta cgaacctaaa taaagctgaa agtgaattct tgggtttttc aataagactc
     2221 cacaaaaagg gaaaaaagaa agtggctttc acgtacgttt gtcgcaaagc agaagaaaac
     2281 attaaaaata aactaaaaca acagattaaa aaaattcaat acgctggagc aaaaaaaaat
     2341 aggcaccgag caataggtga ctacaacatg atggtcatcg gtatccacaa ttactatcgg
     2401 atagcttcac attgtagtgt caatttcact gagatttata gaagtgttag gacagtaata
     2461 gaagcaagac tgaaagctag aggaatgacg aaaaatggaa tctatggaca agatcatgaa
     2521 ggataccgtc cgtatctaaa gtctaagcaa atgagatata ttgaaggttt tccgatactt
     2581 ccaattggct atattaaaaa aagaactcca ctcaacaagc ggagaaatct aaataaatat
     2641 accaaggaag ggagaaagct cattcatcaa gagcaacaag ctatagaaag ctggaaaatc
     2701 aagtatcttg taaaaggttt tagtaacaat aagaaataca cggtggaatt ttctgataac
     2761 aaaatatcta agtttatttc ccaaaaaggg aaatgcgcta tcacaggaaa cgaacttttg
     2821 atggaagaaa ttgaatgcca tcacattaac ccatatcatt tgtccaaaga cgatagttac
     2881 aagaaccttg tcattatctc tagagaaatc catagattaa tccatgcaac tagtgaagaa
     2941 actattctga aactgcttcg gttactgaaa ctaaatcact atcaaattag aaaactaaac
     3001 acgtatcgaa aaaaagcagc taattttcca atatcaatca aaatgaatta gataggaaac
     3061 aaattatatt gtaattaacg atggaacgcc gtgtgcggtg aaagtcgcac gcacggtgtg
     3121 gaccggggga aaagacggag ataatatcaa actcttacct atcggaattg ccaccaatgg
     3181 ataatatgga aggaatcatg acggtcacag ctggccgaaa catgttgtgg gatctgttta
     3241 ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg attatcaaag
     3301 aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc gaagaatttt
     3361 ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat tcattaggta
     3421 tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct gaaag
//
LOCUS       DAJSNV010000158         3398 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSNV010000158 DAJSNV010000000
VERSION     DAJSNV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.163"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..796
                     /locus_tag="ON516_002719"
     CDS             <1..796
                     /locus_tag="ON516_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3346161.1"
                     /translation="ENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATII
                     RFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEK
                     LNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTV
                     NDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLN
                     GGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1479..1577)
                     /locus_tag="ON516_002720"
                     /pseudo
     CDS             complement(1479..1577)
                     /locus_tag="ON516_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1842..2297)
                     /gene="tnpA"
                     /locus_tag="ON516_002721"
     CDS             complement(1842..2297)
                     /gene="tnpA"
                     /locus_tag="ON516_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR3346162.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2540..3398
                     /locus_tag="ON516_002722"
                     /pseudo
     CDS             2540..3398
                     /locus_tag="ON516_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1126 a          585 c          591 g         1096 t
ORIGIN      
        1 agaaaattat ccatttttaa cgaagtctga aaaaaaagtc gctgatttta ttttaaattc
       61 tgggaaaact attatttata gtacgatgag tgatattaag aaaaacacaa aagttggcga
      121 tgctactatt attcgctttt gtcaaaaaat tggtttttct ggtttttctg atttaaaaat
      181 tgaaattgca aaggaagact ttagtcaaaa aaaagaacat ccgactagtg taaagtacta
      241 tgatgaaatt gctaatagtt taacagaagc tctctattcc actattaggc ttttagacga
      301 agaaaaattg aatgaagcca ttaaaatgat cactcaatca aaaaacatat atatatttgg
      361 agtgggatct agtggaaata ctagtcttga tttagaaaat atgtttcttc gcgtaggagt
      421 tcaagcaaaa gctgtactag accctcattt tcaagcccaa gtcgcttctt tattaacagt
      481 taatgattta gtaatcattt tttctttatc tgggaaaacg aaagatactt atgattcatt
      541 gaaaatagct aaaaaaaatg gcgcaaaaat tattgctata actaactata ttcattcacc
      601 tataggaaag tctgcagact tagttttaca aacagccatt gaagaatttc ttaatggtgg
      661 atctttagct ggaaaaatat cacaacttta tatttgtgat ctattagttc atggttatga
      721 acaaaacaat aatattgatt cagttgaatt aagagaaaaa gttttgcgct ctattattga
      781 taaaagtatt gagtgaacta cccccactta gctaaaccta acggttttta acgattgaag
      841 taggcaattc ttgggatgag agcatacttg atagcttatt ttttactaat agcggtggct
      901 cttattgacc aaatatccct gtagttccta cggttcttga ttttatctaa agatattttt
      961 atcgtggaag ctattgatca acttgaaaga aaaacgtgta gctcattatc actagtttat
     1021 cgagtatgga tgaagcaatg gatcatcctc tcttaaataa atttattaag gatctgatcc
     1081 ttcaaatttt ttccatgatc gatgagcagg aacctactga atctaaacga catcgggcac
     1141 aaggaattat taagattgct aagcgaatgt tttctataaa gggtgttcta agctatacag
     1201 tgttaattca aaagattccc aatcatcttg tttataagaa caacgttgaa gattaaaaaa
     1261 gtgtttcaat gctaaaattg caaaatactt caatgttatg agacaaactg tctataagaa
     1321 tcaatttcca cccatttcat cacttgttaa aagtttatga tccttttgat ttttatatta
     1381 atttatctgt atttaataca gattttattt attagttcat ctgcctattg tattttattt
     1441 tgttgcctta ccatctgaag taaatatact cttaattgtc aaattaagtt agacagatca
     1501 tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataatccag tgattttcta
     1561 ggaagattat ttctgcgcca gcattttcta atgtttgaat ttgtgttttg taatcttggt
     1621 tgcttgtgct aattcttgaa tagccgtaaa ttgccaaaat ttcatcaccc tttctggaca
     1681 taagtttctg acaccagtta aaatcaagtc tatcagtaac ccatacaccc gtgaggacgg
     1741 gtgtattttt aatcccccta gaaggggtcg acactcgctg ccccttaaaa ggggattttt
     1801 aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt aaatggttct
     1861 acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg ttcacggatg
     1921 tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct cttcgaccaa
     1981 aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat tagtaccgca
     2041 ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg tactcgaaca
     2101 agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc tttcatttcg
     2161 cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat tacttttcgt
     2221 tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata tgctaaactt
     2281 ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg tagccactca
     2341 ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt agaaacgcgc
     2401 ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa gaaaataaaa
     2461 gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa aatctaactt
     2521 ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa agatcgttca
     2581 tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt ccattaaggc
     2641 tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag agggtctagt
     2701 aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga tagagttata
     2761 tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga acaaccctaa
     2821 tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac tctcaaaaac
     2881 gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga aaaaatctac
     2941 aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac gagtccgttc
     3001 ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac aggaagccca
     3061 acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc gtttaaatta
     3121 gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg gcaaaaacga
     3181 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt tcgggagaag
     3241 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
     3301 aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac gacatacaca
     3361 cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DAJSNV010000159         3284 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSNV010000159 DAJSNV010000000
VERSION     DAJSNV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.164"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            143..832
                     /locus_tag="ON516_002723"
     CDS             143..832
                     /locus_tag="ON516_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3346163.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            846..2348
                     /locus_tag="ON516_002724"
     CDS             846..2348
                     /locus_tag="ON516_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3346164.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2361..2837
                     /locus_tag="ON516_002725"
     CDS             2361..2837
                     /locus_tag="ON516_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346165.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2934..3044)
                     /locus_tag="ON516_002726"
                     /pseudo
     CDS             complement(2934..3044)
                     /locus_tag="ON516_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1078 a          447 c          633 g         1126 t
ORIGIN      
        1 tcaacttttc tccattatac tccaaaataa taaaaaaaac tcctaaatac tccattttta
       61 tctttacttt tatgaaagcg gatgctatat tgtttttgga gttgttatcc aaaaataata
      121 aaaaattgga gtgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg
      181 tttgattgtt tcctgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt
      241 aatgccttat tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc
      301 tgttcgagat attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa
      361 aagagattat ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt
      421 agtagaaacc ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg
      481 actatctata aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat
      541 ggcagatatt tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt
      601 aggaactact ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga
      661 tttgattaaa aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac
      721 tccagaacaa gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc
      781 tattactcga ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa
      841 ataaaatgtt taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat
      901 taccagtagc cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa
      961 atgcttatcc atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg
     1021 ctggaaatgc agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac
     1081 ttgcaaatgg agataaagga acagctggtt tagctggtgg agtagcttat ctagtatata
     1141 ctgcaactat tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag
     1201 gtgtagtggg atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa
     1261 aaattgaatt accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt
     1321 ctagtttagc tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa
     1381 atggtttagt agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat
     1441 atggtttttt attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt
     1501 tctggtatac atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc
     1561 aaaatatttt ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta
     1621 ctagattttt tgcaggaaga tttgcaacaa tgatgtttgg attaccagca gcatgttatg
     1681 caatgtatcg tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg
     1741 gggcccttac atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg
     1801 ttgctccttg gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg
     1861 atatcttaaa tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat
     1921 tcggtgtact tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg
     1981 catgggcatt tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta
     2041 ttcctggaat ggaagaagaa tcagaagttc attctgaaaa agtaataaac aaaaatacat
     2101 cattacatga agagtctctt atcattattg aagctcttgg tggaaaagaa aatattgagt
     2161 ctgttgaagc ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag attattcaaa
     2221 aacaaacaat aaaagacact ggtgcaactg ctgtcttcga agtgaagggt ggcatccaag
     2281 ctgtttttgg agggaaagct gacttattaa gtcaagaaat caatcaaata ttaggaaaag
     2341 ataattagga ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa gaaacttttt
     2401 atgcaccttg ttccggaaaa atcatttcca tcacagaagt aaatgatcct gtcttttcac
     2461 aaaaattact aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt tatgctccat
     2521 tggctggtcg agtaacaaat atatttccta ctaaacatgc tattaatata aaaagcaact
     2581 caggcattga ttatttgatt catattggaa ttgatacagt tgaattacaa ggagagcctt
     2641 tttcgatttg tgttcaagaa aatgatgagg ttactcctga aacctcgttg gttgatgtgg
     2701 attttgacaa aataataaaa tctggtaaag atacttctgt tatagttgtt tttccggaga
     2761 aaaatcagtt caatgattta ataatagata atggtcgaat tgctcatggg aattcatgtg
     2821 gaaaaatcac tttttaaaaa agtgtatata aaaagctata aaataagcta aataggatat
     2881 ttgtcaaatg aagttggtga acagaaagaa ctgttttctt tctgttcatt tttttatttt
     2941 attagtaatg attccccatg ccaaatcatg atccgtaatt taaagttata aaagcttcga
     3001 aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa aatatagaga
     3061 gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa cttaggaaat
     3121 agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca ctatatacca
     3181 aatatagtaa aatgctgaaa gatgtatgga atggcaacag tttttttgac aaattttata
     3241 aggtgcagaa cttctttccg tatgctattc cgattgtcct tgac
//
LOCUS       DAJSNV010000160         3162 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSNV010000160 DAJSNV010000000
VERSION     DAJSNV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.165"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..2555)
                     /locus_tag="ON516_002727"
     CDS             complement(63..2555)
                     /locus_tag="ON516_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3346166.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ON516_002728"
     CDS             complement(2562..3029)
                     /locus_tag="ON516_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3346167.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAJSNV010000161         3157 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSNV010000161 DAJSNV010000000
VERSION     DAJSNV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.166"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(571..1527)
                     /locus_tag="ON516_002729"
     CDS             complement(571..1527)
                     /locus_tag="ON516_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346168.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1636..1881)
                     /locus_tag="ON516_002730"
                     /pseudo
     CDS             complement(1636..1881)
                     /locus_tag="ON516_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2026..2448)
                     /locus_tag="ON516_002731"
     CDS             complement(2026..2448)
                     /locus_tag="ON516_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346169.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2628..2999)
                     /locus_tag="ON516_002732"
     CDS             complement(2628..2999)
                     /locus_tag="ON516_002732"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3346170.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          949 a          693 c          521 g          994 t
ORIGIN      
        1 tacctatgtt ttacgtaatg gtctttagtt atttttgctc ataaaatgca taaaacacgc
       61 gtttttatgt gtgcttgaaa tttcttggct ctataatttc taagactgat aaaaatattc
      121 tatcggttct ttttgcaaat aaaaaaacat atagaagctc cattagattg aatcaccaca
      181 acacaatcaa aggagcattc cgtatgcttg attttcattc tataaaacta cttttaggaa
      241 tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata tccgaagtac cattgtttca
      301 tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt cttatatcgc tctgtcagtc
      361 ctattaataa agtactgttt tgttttcaaa aaaactatta cgagtacttt ttaagagaat
      421 gccttgcaaa tattcatcac acaccgttat ataagtcaaa tagtttcttt cagtcgtcaa
      481 gaaaagattc tcctgtcctt ggcattatca gttaagtagc aacttaattc tcggacttgt
      541 tctactctag caaaatttat tttcatcaac ttacgtccaa atgttttttt gaataattac
      601 ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc actagctttt ggcgtccttc
      661 aggatttcta gtatacacca actgacattt gggcagcgac gattgattta attcgtttag
      721 aaaaaggtta gcgatttgct tatttcggcc aaagcgttct ggtaccgtca gaactatctt
      781 tttcccttcg attaaatacc gcggattctc cagcggtgct aaaagctccc tcatagcttc
      841 agtaaaaagc tgtccttctc gatagccagc attctcaaga tagcatctaa attcctgctg
      901 atcagccgct acgattactt ttctatcact aacttcacca aggcgctgca acgtcgtttg
      961 aacgcatatg gccaagcttt ttttgcgggc acattccttt tcccatagct tttgtcgcct
     1021 acgaatttgg aaatattcca gcaagttacc tttaaatata gctgaccaga agcttagagg
     1081 ctctatattt tttactacta tcggcaatga agtttcttgt ttttgcttta cctgatcgac
     1141 tttcctaacg acgattggca tagataggtg ccagccatca gctaaagcag tactccattg
     1201 ttctttcaac cgacttcgtt cacaagaaaa atagaacatc gactgagtat aagtttccat
     1261 cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg ccaccactct caatttttgg
     1321 tgaagattca aaggtcagac ctgaaatatt gcccaaacgc cgtagcattc ttttcaaatc
     1381 aggctgttca gacagaggaa ctcccggtaa tccaatcgct aaatgccaaa tgttcgttac
     1441 tttattttgt gcacctaggc gtaagccccg ccctcgtagt tgttgtgttt gcacaaaagt
     1501 ggaagcttga ttagccaaga tcaacagatt aattgcagga caatcccagc cttctcctag
     1561 caaactaacg gttcctacca aaatattcaa cttcttttcg gttaataggc gagtaactgc
     1621 ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa taaaaataac ccgacatata
     1681 aggatgttca ttcatacccg tcgctagttc tgccagctca acagttacta gcattttcgg
     1741 taacagtaag aactctccac agatggcacc gatttgctga gtgttaccta atagacgaac
     1801 aacttcatca aacaaaggaa ccacgccata agctaaggta ccatcactgc ctgctaatac
     1861 ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt tcttgcttca gctgaacttc
     1921 ttcttttaac aaaatttgac aaactgcttg ctttttctca ggaatcgcct gactaccaac
     1981 agtgtctgga tactctggaa acaaatgcag cgactcatca aacaatcatc cagctttttt
     2041 caattgataa aaaataggca attccggttg atcttcaaac atctgctgat aaatagtgat
     2101 cagctctttt ttacctaaag atggaactga agctttttta ccttgctgct tagaactgcc
     2161 taatagctgc caatgttcgg gtatcaattc atagttatga tgattcaagt aaattaatgc
     2221 actagtgaac agctcaaaat tttcataaat ttccttcgaa tgctcaagcg gatgctgaac
     2281 aaattctaaa gaaagcaaat aatccaccgt ctcttggctt tccattaacg cttccaccag
     2341 ctcattttgt ttctctaaaa aggtaaaata atgctgagta gtttcctgat tagccggaat
     2401 caaatataga aaatcctgat gaggacatag aacctcctcc tttaccaact cgggaatgct
     2461 aatttcttcg tcaatcaggc cattttaact caatatattg cttccactcc gtctgagagc
     2521 tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag attcggttgt tttttcttca
     2581 agtcaatcaa caattcaccc cagctttttt ttaaatgatg agcttcatca aaaaactaac
     2641 agctccagct gatcaccata agttggctcc tccttcagtc ggttgtaaat ggtctgatac
     2701 gtttccactg taatatgact aggatgttca atcgccccag aaagatagca actatctgct
     2761 ggatttacta agaaatattc ctcgattgtt gttaaccact gtttcttcaa tattagggaa
     2821 ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca tctcaattcc taaaaaggtc
     2881 ttgccagccc ctggtggagc cactagatgg actgaattgt tatttaaata agtagcaaag
     2941 ttagtcaata tacgcttctg atagctacgc cactgaattt tttcatcaga tagccacatc
     3001 ccaggtcccc ctctcacgta ttttctctat cataatctaa atgaatacag caaaaaaaag
     3061 atttcttcaa gaatatgtgt ttttatagta atattttact tactctaaac tcaaatgaaa
     3121 aggtaaaaaa actggtagta caatctttta aattctc
//
LOCUS       DAJSNV010000162         3072 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSNV010000162 DAJSNV010000000
VERSION     DAJSNV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.167"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(334..696)
                     /locus_tag="ON516_002733"
     CDS             complement(334..696)
                     /locus_tag="ON516_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346171.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ON516_002734"
     CDS             complement(770..1150)
                     /locus_tag="ON516_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3346172.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="ON516_002735"
     CDS             1214..2407
                     /locus_tag="ON516_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3346173.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="ON516_002736"
     CDS             complement(2484..3068)
                     /locus_tag="ON516_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3346174.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          505 g         1022 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tg
//
LOCUS       DAJSNV010000163         3011 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSNV010000163 DAJSNV010000000
VERSION     DAJSNV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.169"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..222
                     /locus_tag="ON516_002737"
     CDS             <1..222
                     /locus_tag="ON516_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3346175.1"
                     /translation="FRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKK
                     GNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(359..775)
                     /locus_tag="ON516_002738"
     CDS             complement(359..775)
                     /locus_tag="ON516_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346176.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(778..1062)
                     /locus_tag="ON516_002739"
     CDS             complement(778..1062)
                     /locus_tag="ON516_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346177.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1762..1857)
                     /locus_tag="ON516_002740"
     CDS             complement(1762..1857)
                     /locus_tag="ON516_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3346178.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2510..>3011
                     /locus_tag="ON516_002741"
     CDS             2510..>3011
                     /locus_tag="ON516_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3346179.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEF"
BASE COUNT          958 a          478 c          565 g         1010 t
ORIGIN      
        1 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
       61 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      121 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      181 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      241 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      301 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      361 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      421 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      481 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
      541 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
      601 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
      661 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
      721 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
      781 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
      841 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
      901 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
      961 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1021 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1081 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1141 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1201 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1261 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1321 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1381 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1441 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1501 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     1561 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     1621 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     1681 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     1741 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     1801 agcgcttaaa agcccaacaa gaagggagca aataaagtta gtgaacacga aacacacctc
     1861 cttccagtcg caagactgtc ggataggcga caatcaatta tacaataaaa aagcaaaaat
     1921 aagcctaagt tttttattgc cttggggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1981 ctttagaacg aaggacatag tttttatcga atcttgtctt agaacggctt agacgagctc
     2041 tcaggacgcc acaaaggagt ccctaatgaa ttatttttgg cgaaggagcc taaaggcgac
     2101 tgctgcaagt aaaatgtgag agcgtagcga tcactccttc ttacagcgac catagggagc
     2161 tagattggca gtttaatatc atgcgcgaac ctgtttattt taaaaagtgg gtgatttttt
     2221 ggcgggaaat tttgctcgcg cgcgcgcgtt tattttattt attttttaaa gattttttag
     2281 aacccttttt agggggcagt tgagccttac tctcccaacg gatacagaag ggtactaaca
     2341 actttttatc tgagtactaa caacttttta tctgagtact aacaactttt tatctgagta
     2401 ctaacaactt tttatctgag tactaatatc cttttgtata ataacaacca acctatttac
     2461 gagttggttg ttaaaggtta ttatttattt gtaagggggg aagaaaggaa tggatgaaaa
     2521 aacggaatta acagtcaaat atcaaaatga attgaattta gtaccgctga aaaatttcaa
     2581 tgctaagcag atggatttat tttttgcatt atgcgctcgt atgaaagatc gtggtttgaa
     2641 aaaagttaga ttttctttcg aggaattaaa agagttaagt gattataaga tgactgctac
     2701 aaaagccttt gtcggtgatt tagaaaagct ctataaagat atgcttaatc ttacttaccg
     2761 tactgaagat gaggattcta ttcgctactt tgttttgttt actggttttg aaatcaataa
     2821 aacaaaaaag tttgttgaag tcagtgttaa tccggaccta gaatatatta ttaatggttt
     2881 aacagctgaa tttagtagat ttgaattatc tgctttcacg tcgttgcgtt ctacgtatgc
     2941 aaaaactcta ttccgactgc ttatgcagta ccgttctact ggttattatg tagttcgtat
     3001 tgaagagttt a
//
LOCUS       DAJSNV010000164         2999 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSNV010000164 DAJSNV010000000
VERSION     DAJSNV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.170"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            783..2660
                     /gene="ltrA"
                     /locus_tag="ON516_002742"
     CDS             783..2660
                     /gene="ltrA"
                     /locus_tag="ON516_002742"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3346180.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2791..2999
                     /locus_tag="ON516_002743"
                     /pseudo
     CDS             2791..2999
                     /locus_tag="ON516_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         1135 a          472 c          651 g          741 t
ORIGIN      
        1 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatggt gcgacctgtt
       61 ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact attgtaataa ctcttataac
      121 gagaagtcaa atgagtgggt aacgccacaa agggcttcca ctgatactcc gacatgcaac
      181 aagtagttta tgcagaccaa cgttgtgtga agctcggtga agtcggctga aggataccgt
      241 aaggtaaaag aaatagtgaa taccacgaaa gttctctaga ggtaaagggt gtatccgata
      301 agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac aaacgtactg acgaactagc
      361 gaatgtactg tccgaaaggc atagctccta gaaatgggag agacagtgag caaaaaatca
      421 ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta tgaaagacca tgctgcgtta
      481 caggcgtagg agaagttagg gggcaaaagc tagcatctaa gttaaggcat gtggatagat
      541 ataagggaac aggggaagcc atcaacatgg agagtgcata ctcgatgaag tggtagtaag
      601 aaatatgtga atgacaaata acttacttta gtgatggtga aagtagagtc acgactgatg
      661 aaggttctgt aatggaactg gaggaacagg ctcgattcgt ctgtaaaagt catgaaaccc
      721 gttcaaccat gggattctcg tatgactaaa aaggtcacga gccaattgga gcgtgataaa
      781 ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg gcatgcagaa tattatggca
      841 tgacggaaat cttcgataag atatactcca aaagtttgaa gggagataat ttcaagaatc
      901 taatgcagat tatcatttct gataacaaca ttgtcctagc atatcgtaat atcaagagaa
      961 atcacggtag tgtgacaaga ggagaagatg gagtaacgat taaagatatt gaaaatctca
     1021 accgtaatga attcgtagaa attgttagga agcgattcgc acattataac cctcggaaag
     1081 taaggcgtgt agaaattcct aaatataatg gtaaattaag accgttgggt attccatcaa
     1141 tatgggatag aatcgcgcag caatgcatct tacaagtatt agaaccaata tgtgaagcaa
     1201 aattcaataa tcacagctat ggtttccgcc caaatagatc agcagaacac gcaatagcag
     1261 attgtatggc ccgaatgaat caaagtcaca tggaatatgt ggtagatgta gatatcaaag
     1321 gtttcttcga cgaagtcaat catactaaac tcatgagaca aatctggtct atgggtatac
     1381 gagacaaaca gcttctctta atcattagga aaatgttaag agcacccgta gtacttccaa
     1441 atgggaagtt gatataccca acaaaaggaa caccacaagg cggaatactt agcccattac
     1501 ttgcgaacat caatctcaac gaatttgact ggtggatagc aaatcagtgg gaggaaaaag
     1561 atgccaaaga gctaaaacat cattttagga aaaatggagc ttgggataaa ggtaatatct
     1621 atcacaagct cagaaagagt acaactctaa aagaaatata cctagtgaga tacgcagatg
     1681 attttaaaat tttctgtaga aattataatg aagcgaaaaa agttttctat gcaactaaaa
     1741 tgtggttaca ggagcgtcta aagttaccaa tatctgaaga aaagtcaaaa atcacaaatc
     1801 tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa attagtgacc aaaggtaaca
     1861 aaaaggttgt taatacgcat ataggtacaa aagcacaaag catgattaaa gtgaaactaa
     1921 aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa agataccttt atctatattg
     1981 gagtctataa cagtatggtt attgggattc ataattactt caaaattgcg actcattgta
     2041 gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag ttttgaaata cgactacgag
     2101 aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga aggatacaag ccttacctac
     2161 ggtcaggaca aatacgatat ataagagggt ttccaatatt accagtaggg tatattcaac
     2221 acagacatcc gatgtgtaaa aaaagaaata ttaataaata caccgaagaa ggtagggaag
     2281 ccattcataa gaagcaacaa accgtagaag aatggaagat atcgtggttg tgtagcaatc
     2341 cagtgcttag taagagagca acgattgaat ttgcggacaa tcgaatttca aaattcatcg
     2401 ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat cttatctgaa atgcattgtc
     2461 atcatattat cccttatcat gaaagcaaaa gtgatagcta tgaaaaccta gtgattgtta
     2521 ctgaggaggt tcatcgggta atacacgcta cacaaagtga aacgattgag gaacttctaa
     2581 aatatctaaa attaaatcca aaacaaaaag aaaaactcaa tgagttgaga ttaaaagttg
     2641 gtaacgaaga aatatcttaa taaatatttg taggacggga aaacagaatg tcttttatag
     2701 acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt gtggactggg ggaaaagacg
     2761 gagataatat caaactctta cctatcagta tcgattaatc cagattttat tgaaacggta
     2821 aattatgtca caaactttca acgagaaatg cgtaaagtag aagcaatgac tgttttagct
     2881 acacaatccc cacaagaaat ggtgccagaa aatatgtctt ctgataactt ctcgaaactg
     2941 aagaaagtct ttgaattttc ttctacgaag atctttatgc gtatggatga atctatttt
//
LOCUS       DAJSNV010000165         2985 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSNV010000165 DAJSNV010000000
VERSION     DAJSNV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.171"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            122..205
                     /locus_tag="ON516_002744"
     CDS             122..205
                     /locus_tag="ON516_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3346181.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            330..1067
                     /gene="erm(B)"
                     /locus_tag="ON516_002745"
     CDS             330..1067
                     /gene="erm(B)"
                     /locus_tag="ON516_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3346182.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1330..1446)
                     /locus_tag="ON516_002746"
     CDS             complement(1330..1446)
                     /locus_tag="ON516_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HCR3346183.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            1709..2221
                     /locus_tag="ON516_002747"
                     /pseudo
     CDS             1709..2221
                     /locus_tag="ON516_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2270..2950
                     /locus_tag="ON516_002748"
     CDS             2270..2950
                     /locus_tag="ON516_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3346184.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1013 a          498 c          554 g          920 t
ORIGIN      
        1 tggaaataag acttagaagc aaacttaaga gtgtgttgat agtgcattaa aaattttgta
       61 taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt
      121 catgttggta ttccaaatgc gttatgtaga taaaacatct actgttttga aacagactaa
      181 aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt
      241 atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttgttt
      301 atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact
      361 ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg
      421 ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa
      481 gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat
      541 taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt caattcccta
      601 acaaacagag gtataaaatt gttgggagta ttccttacca tttaagcaca caaattatta
      661 aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct
      721 acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga
      781 ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg
      841 tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg aagctatata
      901 cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt
      961 ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag
     1021 tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc
     1081 ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata
     1141 gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa aaaacagcga
     1201 aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg accccaacat
     1261 gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac tcttttcctt
     1321 ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg taggtggctg
     1381 caagctttcc aatttttgtt gaattgcttt aaattcaggg gtttcttggt gttgattttg
     1441 aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg atactttacg
     1501 atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta agcttgaata
     1561 gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg ttttatgaag
     1621 ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt ttggtggtgc
     1681 cctagctgtc attggatcat ctgcatacaa taagatccat gaaaggccat ctgctgccaa
     1741 tgagcgcctt gagattggta attgggaagc tgatactgta gccggtaaaa caggtagggc
     1801 tcgtattgta actctagtcg atcgtagatc tcgtttctcc ctacttggta aggttgaaaa
     1861 gaaaatctct aaaaatgtgg ttgattgtat gatcaatcta ctaagaaatg taggagaaga
     1921 taaatgtaaa accgttacac ccgatagagg aaaagaattc tcccaacata gtcgaataac
     1981 gctagaatta aatggcacta aagtgtattt tccagatcca catgctccat ggcaacgtgg
     2041 tacaaacgaa aatactaatg gtttattacg agaatattca cccaaaggtg aagatatttc
     2101 aggtattttc gatagtcaaa ttcaagaatg ggcgaataaa ttaaacacgc gaccacggaa
     2161 atgtctaaat tggaaaaccc cttatgaagt atttttggtt ctgttgcaaa gttccccata
     2221 actcctgatt tattgtaaaa tgtaagaaaa cgagagtgag ggtagacaga tgaatcattt
     2281 caaagggaaa caatttcaaa aagatgtgat tattgtcgct gttggttact acctgcgtta
     2341 caatctgagc tatcgtgaag ttcaggaatt actgtatgat cgcggaataa atgtttgtca
     2401 cactacgatt tatcgttggg ttcaagagta cagtaaaatc ctctatcatc tttggaagaa
     2461 gaaaaataga cagtccttct actcgtggaa aatggacgag acctatatca aaataaaagg
     2521 ccattggcat tatctctatc gtgcgattga ttctgatgga ttaactttag acatttggtt
     2581 acgaaagaaa cgggatactc aggcggctta tgctttctta aaacggctcc ataaacaatt
     2641 tggacaacct agagtgattg taaccgataa agcaccatcg attagctctg cattcagaaa
     2701 gctacagatt aacggtttat ataccaagac agaacatcgg acagttaagt atctcaataa
     2761 tttaattgag caggaccatc gtccaattaa gcgccgtaat aaattttatc gaagtttacg
     2821 aactgcctca accacgatta agggcatgga gactattcga ggaatataca aaaagaaccg
     2881 aagaaacgga acgctcttcg gattttcggt gtcgactgaa attaaagttc tgatggggat
     2941 cccagcatag tcaaaatatt aaactaggac ttctatactc ttttg
//
LOCUS       DAJSNV010000166         2945 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSNV010000166 DAJSNV010000000
VERSION     DAJSNV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.172"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="ON516_002749"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="ON516_002749"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3346185.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="ON516_002750"
     CDS             complement(1061..1981)
                     /locus_tag="ON516_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3346186.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="ON516_002751"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="ON516_002751"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3346187.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          823 a          601 c          509 g         1012 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaact
//
LOCUS       DAJSNV010000167         2889 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSNV010000167 DAJSNV010000000
VERSION     DAJSNV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.173"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON516_002752"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON516_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3346188.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          834 a          567 c          441 g         1047 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattctt
//
LOCUS       DAJSNV010000168         2857 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSNV010000168 DAJSNV010000000
VERSION     DAJSNV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.174"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            562..843
                     /locus_tag="ON516_002753"
     CDS             562..843
                     /locus_tag="ON516_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346189.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="ON516_002754"
     CDS             845..1261
                     /locus_tag="ON516_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346190.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="ON516_002755"
     CDS             complement(1422..1688)
                     /locus_tag="ON516_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346191.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="ON516_002756"
     CDS             complement(1646..2620)
                     /locus_tag="ON516_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3346192.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DAJSNV010000169         2820 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSNV010000169 DAJSNV010000000
VERSION     DAJSNV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.175"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            95..1054
                     /locus_tag="ON516_002757"
     CDS             95..1054
                     /locus_tag="ON516_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HCR3346193.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ON516_002758"
     CDS             complement(1487..2059)
                     /locus_tag="ON516_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346194.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="ON516_002759"
     CDS             complement(2075..2680)
                     /locus_tag="ON516_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR3346195.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          468 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
//
LOCUS       DAJSNV010000170         2780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSNV010000170 DAJSNV010000000
VERSION     DAJSNV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.176"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..197
                     /locus_tag="ON516_002760"
     CDS             <1..197
                     /locus_tag="ON516_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346196.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="ON516_002761"
     CDS             314..811
                     /locus_tag="ON516_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3346197.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="ON516_002762"
     CDS             786..1292
                     /locus_tag="ON516_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3346198.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="ON516_002763"
     CDS             1276..>2780
                     /locus_tag="ON516_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3346199.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DAJSNV010000171         2709 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSNV010000171 DAJSNV010000000
VERSION     DAJSNV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.177"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            143..1210
                     /gene="rlmN"
                     /locus_tag="ON516_002764"
     CDS             143..1210
                     /gene="rlmN"
                     /locus_tag="ON516_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3346200.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1403..2506)
                     /locus_tag="ON516_002765"
     CDS             complement(1403..2506)
                     /locus_tag="ON516_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3346201.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          862 a          483 c          547 g          817 t
ORIGIN      
        1 cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc
       61 gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt
      121 tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga
      181 attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga
      241 atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt
      301 gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga
      361 agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac
      421 tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa
      481 tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg
      541 agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga
      601 acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt
      661 aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat
      721 tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca
      781 agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg
      841 gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa
      901 aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga
      961 gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt
     1021 gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt
     1081 attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca
     1141 tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt
     1201 tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa
     1261 ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt
     1321 ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct
     1381 tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg
     1441 tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat
     1501 aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg
     1561 tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca
     1621 catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat
     1681 aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat
     1741 agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg
     1801 tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac
     1861 tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt
     1921 ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga
     1981 cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa
     2041 taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca
     2101 ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa
     2161 tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc
     2221 aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga
     2281 tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct
     2341 ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc
     2401 ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg
     2461 catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc atttccttct
     2521 ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg
     2581 gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa
     2641 tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa
     2701 tttttgttc
//
LOCUS       DAJSNV010000172         2650 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSNV010000172 DAJSNV010000000
VERSION     DAJSNV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.178"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            36..197
                     /locus_tag="ON516_002766"
     CDS             36..197
                     /locus_tag="ON516_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346202.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="ON516_002767"
     CDS             210..713
                     /locus_tag="ON516_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3346203.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="ON516_002768"
     CDS             773..1162
                     /locus_tag="ON516_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3346204.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="ON516_002769"
     CDS             1149..>2650
                     /locus_tag="ON516_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3346205.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DAJSNV010000173         2643 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSNV010000173 DAJSNV010000000
VERSION     DAJSNV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.179"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="ON516_002770"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="ON516_002770"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3346206.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DAJSNV010000174         2545 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSNV010000174 DAJSNV010000000
VERSION     DAJSNV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.180"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(986..1504)
                     /locus_tag="ON516_002771"
     CDS             complement(986..1504)
                     /locus_tag="ON516_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR3346207.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          828 a          463 c          363 g          891 t
ORIGIN      
        1 atttttacta taccctatac ttttacttgg tatatttatg aataatcgca aatttacccc
       61 tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt tcttttatta
      121 aatacaccta caacttataa attgatggta tttagataac acgaaagaat aaaaataaca
      181 gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa
      241 cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa tcggtactgg
      301 gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct actatttgat
      361 ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg tatagaattt
      421 atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac cctttgatgg
      481 ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta acaaaggcta
      541 tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt cttcctaacc
      601 caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt gttgagattc
      661 catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca aaaataaaaa
      721 aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc ataatctcat
      781 tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca atctaacgat
      841 gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa accggcagtt
      901 agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt ctcctttcca
      961 caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt cttatctttg
     1021 ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat acttcccata
     1081 cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat agatcttcat
     1141 cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga atttcctcta
     1201 attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac tctcctaact
     1261 gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt tcatcatttt
     1321 cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca gcaggtgttc
     1381 tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca aattctaaaa
     1441 tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt ttatatgttc
     1501 tcattatatt acctccataa attcctaatt gtctccttta attcatctat aatttcatcc
     1561 acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc taactcatat
     1621 gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc gtcagcaagg
     1681 agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg catatcattt
     1741 tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa ctaccacttt
     1801 aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc cattgttaaa
     1861 cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc gctctttctt
     1921 cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca cgttccttac
     1981 ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct
     2041 aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc aagatttacc
     2101 tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca tttataatac
     2161 tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac agatacactt
     2221 gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta atcttccgaa
     2281 atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa
     2341 aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg ttgacacggt
     2401 gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc taaaaagtct
     2461 atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc aacttatatg
     2521 ttttagtgaa ctacccccac ttagc
//
LOCUS       DAJSNV010000175         2468 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSNV010000175 DAJSNV010000000
VERSION     DAJSNV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.181"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1262)
                     /locus_tag="ON516_002772"
     CDS             complement(<1..1262)
                     /locus_tag="ON516_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3346208.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTG"
     gene            complement(1425..2018)
                     /locus_tag="ON516_002773"
     CDS             complement(1425..2018)
                     /locus_tag="ON516_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346209.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2019..2351)
                     /locus_tag="ON516_002774"
     CDS             complement(2019..2351)
                     /locus_tag="ON516_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346210.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
BASE COUNT          654 a          527 c          408 g          879 t
ORIGIN      
        1 cctgtgactt gcgtatcagt atttgaacgc gaatttgagg aagtaaagcc gcgtggtact
       61 gttccatact cttcataaaa acgatttaag taggtcttaa attctgcttt catcgttcct
      121 tttagctgag gactcactaa ctgtacctga tagaccactt tgtctaagtg tgtttgatag
      181 gacgctgctt cattgatctt caaatctttg actgatccat cgtcatataa actggatgct
      241 tcatcatacg tccgtgtggc aaatatctct aaaggattga tcattccgtc agatccgtct
      301 aaacgcacca taataccgtt atattccttt aagaaaggaa taaagtcttt tcccttttca
      361 aagccccaaa taaagtctcc tgcagctaaa tgggcttccg cgatcatttt aagaacggtc
      421 gatttaccag ctcccatcat tcctagaagc aaggtattag tagacaaccg tacactattt
      481 ttatacgtcg cattccagac aaatgggccg cctgtcatgg tttccccata ttgtgtccct
      541 cctggatctt ttagcgtttg gtggtggaac gggaaacctc cgccaatgtt taacgatggt
      601 agtgattgcc ctttgatatt tccgaaccat tcagcttgtt catcgaacat ggtaaacatc
      661 gattgccaat ctcgtttggt tttatgcaga taagtcaccg cacgataatc catccctttg
      721 agattttcga tcagttcctg cgctcgttca tctacttctt gaatcgtgtt tccgtaaaca
      781 tgtactctca ctttgacttg cttagaaatt tccccattag aagtcaattt tctagcatat
      841 tccgctagtt ccaggtattc ttccgcgctt ttatttcggt cagtcgcttt tcgttcatta
      901 tctgatcgat catttaattc atctaaggca cgattgattt gatcaatcac tttttgcttt
      961 tcatctgtgc taacatcgag tgtggccact gtatgaggat tgttcatgag attggccaac
     1021 caaaaaagcg taggattttt tgtgtagtga taaactgtca cacatcgtga ataaccatta
     1081 ccatacgtca catattcttc atcaaaattt aacccacctt gtggcatcac ttgttttaaa
     1141 aaatctaaat catacccttt ggattttaca atttctattt cccgatttcc tagttgcttt
     1201 aatggatcaa gtactttttg atcattttgt ggtgaaaaag ctgttttaat tgcttctaac
     1261 atgtacagtc acctcttctt gtttgattct ttacttggtt aaaagattgc ccttttccca
     1321 acttacccag gcttaaagtg tgaggaaatt ttctagtggt ctcttccttc ggaacatctt
     1381 tttctaacca tatcaacgga tatttcattc tctcttctcc attcctactc atcattcata
     1441 ttgttcattc gatacagaat cttgatcaat tcttttttgt agagtggttt taaccctaaa
     1501 atgccactag atcgcattaa ctgacggttt ctggtttgta aatccttgat tgtctttcct
     1561 tcaatgacga aaaagaagct taagtcagga cgttcttgtt ctgctcgttt caattcctgg
     1621 atttcttcga ctaatttctt ttcatatttt tttaatcgtt gtagatttct tcctaaggac
     1681 tgttccatac gatttttatc taataattgc cgcttttgca cgatttgttc cgttagatcc
     1741 gtctctgtgg tcaaactaac taacttaatt gttggcacat acgtacgcat gacggtggtc
     1801 aactgattca ttcgttctag tttctcttgt tcatttaatc cttccacgtc tgtagaaact
     1861 acacgataat aacgttgaaa atgatcttct ctagtaatta agactccctc ttctgtacag
     1921 gatttatatg gaaataagtc caagatagag cgctcttctg gttttccaaa ctgcaaaatg
     1981 gatttccttt caactgaatt ttgtcctttg actcgcattt attcatcctc cttatacgta
     2041 ccatgataat ttcggttagg atctaacgca tgataatggt tcttgtccgc aaaaaaaata
     2101 gactcgataa tcgcaaaaat ttgtcgttga cctgggttat tatagggtcg ccaaacaatc
     2161 actaacgcta tcgcaatcca taagccataa ttcaagagtg caaaaaagag tggtaagtgg
     2221 acaagattat tgaccatttt cgcccaaaaa aagcagccac caagcgttaa tagatctaaa
     2281 aagaaaaaag cgaaaaactt ccattctcca ctaaatcttg gagggttttt catgttacta
     2341 tatttatcca taaatgattt ctccttttca tacatagaaa aaaggcctaa cttgtaggcc
     2401 ttttatggtt tgatatcctg tttgatatcc ctcaatagtt gttctttcaa tgcattgttc
     2461 ctgttttt
//
LOCUS       DAJSNV010000176         2456 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSNV010000176 DAJSNV010000000
VERSION     DAJSNV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.182"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1262)
                     /locus_tag="ON516_002775"
     CDS             complement(<1..1262)
                     /locus_tag="ON516_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3346211.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKAKGYDLDFVKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTG"
     gene            complement(1425..2018)
                     /locus_tag="ON516_002776"
     CDS             complement(1425..2018)
                     /locus_tag="ON516_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346212.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2019..2351)
                     /locus_tag="ON516_002777"
     CDS             complement(2019..2351)
                     /locus_tag="ON516_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346213.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
BASE COUNT          661 a          522 c          403 g          870 t
ORIGIN      
        1 cctgtgactt gcgtctcagt atttgaacgc gaatttgagg aagtaaagcc gcgtggtact
       61 gttccatact cttcataaaa acgatttaag taggtcttaa attctgcttt catcgttcct
      121 tttagctgag gactcactaa ctgtacctga tagaccactt tgtctaagtg cgtttgatag
      181 gacgctgctt cattgatctt caaatctttg actgatccat cgtcatataa actggatgct
      241 tcatcatacg tccgtgtggc aaatatctct aaaggattga tcattccgtc agatccgtct
      301 aaacgcacca taataccgtt atattccttt aagaaaggaa taaagtcttt tcccttttca
      361 aagccccaaa taaagtctcc tgcagctaaa tgggcttccg cgatcatttt aagaacggta
      421 gatttaccag ctcccatcat tcctagaagc aaggtattag tagacaaccg tacactattt
      481 ttatacgtcg cattccagac aaatgggccg cctgtcatgg tttccccata ttgtgtacct
      541 cctggatctt ttagcgtttg gtggtggaac gggaaacctc cgccaatgtt taacgatggt
      601 agtgattgcc ctttgatatt tccgaaccat tcagcttgtt catcgaacat ggtaaacatc
      661 gattgccaat ctcgtttggt tttatgcaga taagtcaccg cacgataatc cattcctttg
      721 agattttcga tcagttcctg cgctcgttca tctacttctt gaatcgtgtt tccgtaaaca
      781 tgtactctca ctttgacttg tttagaaatt tccccattag aagtcaattt tcgggcatat
      841 tccgctagtt ccaggtattc ttccgcactt ttatttcggt cagtcgcttt tcgttcatta
      901 tctgatcgat catttaattc atctaatgca cggttgattt gatcaatcac tttttgcttt
      961 tcatctgtgc taacatctag tgtggccact gtatgaggat tgttcatgag attggccaac
     1021 caaaaaagag taggattttt tgtgtagtga tagactgtca cacatcgtga ataaccattt
     1081 ccataggtca catactcttc atcgaaattt aatccacctt gtggcatcac ttgttttaca
     1141 aaatctaaat catacccttt ggcttttaaa atttctattt cccgatttcc tagttgcttt
     1201 aatggatcaa gtactttttg atcattttgt ggtgaaaaag ctgttttaat cgcttctaac
     1261 atgtacagtc acctcttctt gtttgattct ttacttggtt aaaagattgc ccttttccca
     1321 acttacccag gcttaaagtg tgaggaaatt ttctagtgat ctcttccttc ggaacatcat
     1381 tttctaacca tatcaacgga tatttcattc tctcttctcc attcctactc atcattcata
     1441 ttgttcattc gataaagaat cttgatcaat tcttttttgt agagtggttt taaccctaaa
     1501 atgccactag atcgcattaa ctgacgattt ctggtttgta aatccttgat tgtctttcct
     1561 tcaatgacga aaaagaagct taagtcagga cgttcttgtt ctgctcgttt caattcctgg
     1621 atttcctcta ctaatttctt ttcatatttt cttaatcgct gtagatttct tcctaaggat
     1681 tgttccatac gattcttatc taataattgc cgcttttgca cgatttgttc cgttagatcc
     1741 gtctctgtgg tcaaactaac taacttaatt gttggcacat acgtacgcat gacggtggtc
     1801 aactgattca ttcgttccag tttctcttgt tcatttaatc cctccacgtc agtggaaacg
     1861 acacgataat aacgttgaaa atgatcttct ttagtaacta agactccctc ttctgtacag
     1921 gatttataag gaaataaatc caagatagat cgttcttctg gttttccaaa ctgcaaaata
     1981 gatttcttat caactgaatt ttgtcctttg actcgcattt actcatcctc cttatacgta
     2041 ccatgataat ttcggttagg atctaacgca tgatagtggt tcttatccgt aaagaaaata
     2101 gattcgataa tcgcaaaaat ttgtcgttga cctgggttat tatagggtcg ccaaacaatc
     2161 actagagcta tcgcaatcca taagccataa ttcaagagtg caaaaaagag tggtaagtgg
     2221 acaagattat tgactatttt cgcccaaaaa aagcaaccac caagcgttaa tagatctaaa
     2281 aagaaaaagg cgaaaaactt ccattcccca ctaaatctag gaggattttt catgttaata
     2341 tatttatcca taaatgattt ctccttttca tacatagaaa aaaggcctaa ctcgtaggcc
     2401 ttttatgatt tgatatcctt caataattgt tctttcaatt cattgttcct gttttt
//
LOCUS       DAJSNV010000177         2453 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSNV010000177 DAJSNV010000000
VERSION     DAJSNV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.183"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(69..971)
                     /locus_tag="ON516_002778"
     CDS             complement(69..971)
                     /locus_tag="ON516_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3346214.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ON516_002779"
     CDS             complement(968..1660)
                     /locus_tag="ON516_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3346215.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ON516_002780"
     CDS             complement(1703..2344)
                     /locus_tag="ON516_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3346216.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT          655 a          560 c          408 g          830 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaa
//
LOCUS       DAJSNV010000178         2399 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSNV010000178 DAJSNV010000000
VERSION     DAJSNV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.184"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            206..2068
                     /locus_tag="ON516_002781"
     CDS             206..2068
                     /locus_tag="ON516_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346217.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="ON516_002782"
     CDS             2068..>2399
                     /locus_tag="ON516_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346218.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DAJSNV010000179         2362 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSNV010000179 DAJSNV010000000
VERSION     DAJSNV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.185"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            75..2228
                     /locus_tag="ON516_002783"
     CDS             75..2228
                     /locus_tag="ON516_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3346219.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          807 a          419 c          524 g          612 t
ORIGIN      
        1 cccatttttt cttttttatt aagaagtttt tgggtacaat gaagcatgaa tgtaaatagg
       61 cggaaggata ggaaatgatg aaagatttta tgaaaaaaat attaaatact tcttggttag
      121 aaaaaataag aaaaggtgca caagaaccta agaacaaaac gtttcgccgg tcccatgttc
      181 cttttcgctt aaattttctt tttttcatta tatttacgtt atttgttgcg ttgattgcta
      241 gattgggtta tttgcagatc gtgaacggcg aagagatgga ggcgcgagtg aagtctactt
      301 ctacgattac ggtccaagaa agtactcctc gcggaatgat ctacgactcc acaggaaaag
      361 ctttagttac gaataaagcg aatcaggcca tcacatatac gagaggaaca caagttacgg
      421 ctaaagattt gcttgaaatc gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg
      481 aaaatctgac agatagagat aaaaaagatt tttggctggc tgatcccaaa catttgaaag
      541 aagccacaaa aagactttcg gcaaaagaaa agcaattagg gaacagcgaa caatatagtg
      601 cgatggtcaa taaagtcaca gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg
      661 ctaccatctt taaacggatg aatagtgcat atgaattaaa cactgtctat atcaaaaact
      721 ctggtgttac tgatcaagag ttagctgttg tggcagaacg cgcttctgaa cttccaggag
      781 tttcgactgg cacagactgg acacgagaat ataatgcagc aagttcactg aaaagtattc
      841 ttggcagtgt gacgactgaa aagcaaggac ttccagcaga tgaagcagag aaatacctag
      901 caaaaggtta ttctcggaat gaccgtgtag gacaaagtta tttggaaaaa caatatgaag
      961 atgtcctgca aggaaccaaa acccaatatg aagtttccct tgataatgaa ggaaatgttt
     1021 cgaatcaaaa agaaatcttc tctggggaaa aaggttctaa tttaatgtta tctatgaatg
     1081 cagaattcca atcaaaagta gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca
     1141 acggcaaagc acagtattcg cctggtgctt atgcagtggc tatgaatcct cagacaggag
     1201 aagtgctagc aatgactggt ttcagtcatg aacaaggttc caaagaaatc accgagaatg
     1261 ctttaggcac gattactagc gcctttgatc cagggtctgt agtcaaggct ggtactttga
     1321 cagcaggctg ggcaagtaac gcgatcagcg gaaatcaagt tttgattgat gagccgattc
     1381 gtctgcaagg tgcttcagaa aaaagctctg tctttaatcg aagcggacaa gttgctcttg
     1441 atgcagtcaa agcatcggaa ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga
     1501 tgttaggact agactataca cctggaatgg gactacctag tttagatgaa gaagctaaag
     1561 cttatcagca attgagagac tctttcaaag agtttggtct tggtacaacg actgggatcg
     1621 atttgccgaa tgaatctcct gggatctcac gttctgttga ttatatgaag aagttcaatg
     1681 cggataatgg caaagaatgg tatgctccag gtaactttac cgatttggca tttggacagt
     1741 ttgacacata tacaccaatc caattagctc aatatgcttc aacagtcgct aatggcggga
     1801 aacgagtaca gccgcaccta gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg
     1861 gtgaagtgaa gaaagaaatt ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa
     1921 atatggcgat cctccgtgaa ggattccacc aagttgtcca tggtaccgat ccttacacaa
     1981 cagccaaacc attagccagt gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa
     2041 cggttgcgga aggtcatcca gatattgcga cagtcaatag taatatcgtg gcatacggac
     2101 caacagacaa tccggaaatt gccatcagtg ttgtcttgcc gaacttatta gacgaacaag
     2161 accacataaa cttgacgatt gctaaagaaa taatggacgc gtattatgat atgttcatgg
     2221 caaaataaaa aagaggtcgt gaaaaagccg cttagtttcg agtatgaaga aaaattttgt
     2281 gaaaatagct tctcacattt ttgcttaaat ttgacttcat accgagaaac tggcttttga
     2341 acaccgttta ctcggcaaaa aa
//
LOCUS       DAJSNV010000180         2306 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSNV010000180 DAJSNV010000000
VERSION     DAJSNV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.186"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1336
                     /locus_tag="ON516_002784"
     CDS             <1..1336
                     /locus_tag="ON516_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3346220.1"
                     /translation="KSLIIILVILSMSTLSLISLSIKDATDRASKETFANITNSFSME
                     INRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQ
                     SPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNN
                     LKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIH
                     TAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQ
                     QSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFL
                     IEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEG
                     FNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1622..>2306)
                     /locus_tag="ON516_002785"
     CDS             complement(1622..>2306)
                     /locus_tag="ON516_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346221.1"
                     /translation="LYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLN
                     QCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMR
                     WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTS
                     LEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFL
                     WLFRMGVIP"
BASE COUNT          823 a          344 c          414 g          725 t
ORIGIN      
        1 aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc tcattagttt
       61 atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata taacaaatag
      121 tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg ggggaaatgt
      181 aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct atgtaaaaag
      241 aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag agactcttgc
      301 gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa ctggggttaa
      361 cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag aaggaaagca
      421 tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg aaaaaaataa
      481 ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg ataatgagaa
      541 aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc ataatagcgg
      601 tgtagtaagt gcagcacaag aactatacga aaatacatta atcactgata ttcatacagc
      661 tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa cattctttgt
      721 aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag atataaattg
      781 gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac aatctatatc
      841 aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg caggagttgt
      901 agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag cagtattact
      961 atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga tggtatttat
     1021 atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg acaagcttgg
     1081 gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta aacaatctgc
     1141 atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat tatcaagttt
     1201 agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga gtttagtact
     1261 tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa aagaattatt
     1321 aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag tgtaatataa
     1381 accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt atttttctaa
     1441 aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg atgctgagaa
     1501 gttagctgaa tccattttga atacttatca acccgaatct gtagaggata tgcaagatgt
     1561 tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat tgaataacca
     1621 tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac gaaacggtta
     1681 tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc ccagagaccg
     1741 ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct ccgacatcgc
     1801 agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta
     1861 cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg
     1921 ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca
     1981 agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca aatattcgtt
     2041 caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg cgaagatgtt
     2101 ccacttcatc catcagatct ccccaaacat gtctcgtgat gactcgttga tgatttttac
     2161 ttatggtaca ttggtttagc agaggacagt tcacacattc ttttggattg gatttgtatt
     2221 ctcggtaccc ttctcgagta accgttgaaa aagcgagcat ttgatcttgc ggacaaatat
     2281 aacaatcaaa atactcgtca tagaga
//
LOCUS       DAJSNV010000181         2286 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSNV010000181 DAJSNV010000000
VERSION     DAJSNV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.187"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(313..903)
                     /locus_tag="ON516_002786"
     CDS             complement(313..903)
                     /locus_tag="ON516_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3346222.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1426..1587
                     /locus_tag="ON516_002787"
     CDS             1426..1587
                     /locus_tag="ON516_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3346223.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1606..2100
                     /locus_tag="ON516_002788"
     CDS             1606..2100
                     /locus_tag="ON516_002788"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3346224.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2093..2254
                     /locus_tag="ON516_002789"
     CDS             2093..2254
                     /locus_tag="ON516_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346225.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          809 a          373 c          405 g          699 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagt
//
LOCUS       DAJSNV010000182         2269 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSNV010000182 DAJSNV010000000
VERSION     DAJSNV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.188"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            190..444
                     /locus_tag="ON516_002790"
     CDS             190..444
                     /locus_tag="ON516_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3346226.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            445..762
                     /locus_tag="ON516_002791"
     CDS             445..762
                     /locus_tag="ON516_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3346227.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1321..2256
                     /locus_tag="ON516_002792"
     CDS             1321..2256
                     /locus_tag="ON516_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346228.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          428 g          711 t
ORIGIN      
        1 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
       61 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      121 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      181 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
      241 cttccaagct ctcttggatt aaaaagcgga gacattatcc aagccaaaca agaaggcaac
      301 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
      361 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
      421 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttt
      481 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct ttctccagcc
      541 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
      601 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
      661 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
      721 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggaaaaagt
      781 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
      841 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
      901 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
      961 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1021 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1081 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt gttttaaaag
     1141 aggaagtcca atatatctat gggaaacttc ttaaatatta tgttggtaca tgaaaatata
     1201 catccctgct agactattta caacatttta ctttgtacgt attcttgttt taaaaggaat
     1261 gggataaaat ataggtacaa taactatttg atgctaaata taattaatgg aggagagata
     1321 gtgaacatac ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata tattactact
     1381 ttcaaaagat tatccgagtt aatgcagcag actgttaaaa gtgctatgcc tgaaggatac
     1441 aagtttaatt ttgttcaatc ttcagtatct actgaaagta tctatttttc aattctttct
     1501 gagagtaggc tttttcattt tagaataagt tctcatgtaa agaaaactga tgttgatata
     1561 tatacgttcc gtacaaataa ttataaaaat ttaaatgatt tgagtagaag tatcagtgaa
     1621 tatctcaagg ggaatagcta tctcaagatt cgattttcac atatattaat tttactacca
     1681 tatttgaatg agaatttcac cctaattcaa gtagataagt ctttcattgc ctcaggtaat
     1741 caaggaaata tgaatattac aagtaacaaa acgtcacgtg tgctattggc gttgatgggt
     1801 tacggaattc tttatagaga tgaccaaaat ggccagattc taataaatcg ggctgggcgt
     1861 tcattattaa tgaaatatag aaaatttata ccggaagatt ttcaaaactt acagttagaa
     1921 aacatgcgtt tagaagattt atttagatta gcagaagatg ataagtatag atttgaagtt
     1981 ttgaaaattc aaaagaatga actattgaat aagttatatc ctgagaatgc gataaaagca
     2041 cattttggtg atgctaatca tatgtatagt tggttcattc ctaaaagatt tagagggaaa
     2101 atcaatgctg gagatcaagt gaaagtactc aatcgaaata gtacagatgt cgtaatgata
     2161 acagatatct atgtagaaga tgaagatata atcaaatata tgaagcctgt gataaaactt
     2221 gttaataaaa ataaaggaac ccgtggtgct agttaatttt ttcctagag
//
LOCUS       DAJSNV010000183         2268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSNV010000183 DAJSNV010000000
VERSION     DAJSNV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.189"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..>2268
                     /gene="acm"
                     /locus_tag="ON516_002793"
     CDS             137..>2268
                     /gene="acm"
                     /locus_tag="ON516_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR3346229.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICA"
BASE COUNT          889 a          348 c          446 g          585 t
ORIGIN      
        1 ttataaatat ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg
       61 gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat
      121 aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat
      181 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
      241 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      301 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      361 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      421 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      481 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      541 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      601 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      661 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      721 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      781 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      841 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccggcg ctacatttaa
      901 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
      961 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
     1021 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
     1081 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
     1141 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1201 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1261 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1321 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1381 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1441 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1501 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1561 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1621 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1681 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1741 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1801 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1861 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     1921 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     1981 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     2041 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     2101 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     2161 tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa
     2221 atctagtttt gctgtaatca ttggtttatt gatagtaact atctgtgc
//
LOCUS       DAJSNV010000184         2228 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSNV010000184 DAJSNV010000000
VERSION     DAJSNV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.190"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..280)
                     /locus_tag="ON516_002794"
     CDS             complement(<1..280)
                     /locus_tag="ON516_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3346230.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ON516_002795"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ON516_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          655 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 tgtaaaaaac tattgcacaa tacaagtagc ctacggctat cgaaatttcc ataactttcg
     2221 agcaagaa
//
LOCUS       DAJSNV010000185         2224 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSNV010000185 DAJSNV010000000
VERSION     DAJSNV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.191"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..153)
                     /locus_tag="ON516_002796"
     CDS             complement(<1..153)
                     /locus_tag="ON516_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3346231.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ON516_002797"
     CDS             complement(475..696)
                     /locus_tag="ON516_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346232.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ON516_002798"
     CDS             complement(794..1039)
                     /locus_tag="ON516_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346233.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ON516_002799"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ON516_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          746 a          393 c          318 g          767 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tatatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttat
//
LOCUS       DAJSNV010000186         2202 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSNV010000186 DAJSNV010000000
VERSION     DAJSNV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.192"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            378..833
                     /locus_tag="ON516_002800"
     CDS             378..833
                     /locus_tag="ON516_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346234.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(995..2167)
                     /locus_tag="ON516_002801"
     CDS             complement(995..2167)
                     /locus_tag="ON516_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3346235.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          711 a          439 c          393 g          659 t
ORIGIN      
        1 taataacaga actatagcaa aaaatcccgt gtatcaaaac gggattagat taagtttcct
       61 caatcactat tcataacaca attattataa caagtaagta aacaattgaa aaggacattt
      121 attgaaaacg gttgtttttt acttaaggag gaaatttagt gaaaaaatta gttgcattgt
      181 ttttggtcgc aggaagtttg ttgagtgggt gtggtgctac tacgtctact actcaaagca
      241 caagttcagc tcaggcttcc agcagcagta ccacgatctc cgaaagtacg gcagattcat
      301 cgaaagaaca agaacaaatt gaaaagaaaa aagaagaaga gaaacaaaaa gcggaaaaag
      361 ctgcactaga aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc
      421 ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac
      481 aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag
      541 aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag
      601 caatcgcaca gcaacaagca gaacaggcgc gacagcaagc agcagctgaa caggcctctc
      661 agcaggcaca agccgcagct cctgagcaaa ataatgaaca aactgtctat gtcacaccga
      721 ccggttcaaa ataccataca cataaatgtg gaaatggtac gtatacccct gccactttaa
      781 gcgaagctca agctcgtggt ttaacccctt gctccaaatg ttatggaaac taattcaaaa
      841 gttatactat aataataaaa ttcttctgaa acataaaaaa atagaagctg gcgttccaga
      901 tagtaaacca aaaatagcag tttacacgtc catttcgtca gcttctctct tttttcattt
      961 agtctgttcc ctctgctttt ttggacatct tcattcattc tttctcaatc aaatagtaca
     1021 cctctttttc ttttcgcagt ttatatcttc cttttttcaa tttcattgaa aactgtttcg
     1081 aatgtttatg ttttacatgg attttaagca ggagctcctc atgttgcaag cgatagttcc
     1141 tgccttttaa atcagaaaat ctcactagtc cgtattcatc agaagctttc acaaatggaa
     1201 ctttttctct agtaatatag tgtttgccgt tgagcgtttt tacagaaaag accattcctt
     1261 cgattggtcg tccgtttgtg ttctttacat aaaaagataa aggatagttt cgcttagcga
     1321 tggccaaaga atctaaataa gcgatgagta gccaaactaa gccgattgat ccaagtagta
     1381 taccgataaa ccaaataaaa aatttccatt ttttccattg atcaattcct tggatcgctt
     1441 tttttgcctc ttttgcttga taaggaatac gatgccccgt cacaagcagt cgatgtgtat
     1501 tgaccatata aggagtacag gtaagcaaag tcacgagatc tctgcctttt tggataccca
     1561 aagaatctac ttcatcaggt aagaccactt ggattttttc tacttgatac gccaatgtct
     1621 ttccattgat ctggatgaaa aactggtctc cttttttcaa cttaggcaaa tctgtaaata
     1681 attttgcttc tgggagtcca cgatgcccag aaagaacggc atgggtgttt ttcccgccag
     1741 tcggataaga actttcttcc aataatgacg ttcctttttg caaaaatatt tccgttgtct
     1801 gatcgaatat aggtaagctg acattgattt ttgggatttc gatgactcca agcatatgtt
     1861 ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt tgcatcaact gcttggttga
     1921 aactgcctat ccctggtgat acgttttgct ctgccaactg ttggtttttc tttgtcattt
     1981 tttcctgctg tttttttatt tcctctgaat tcttattcga tgcttgcttt tgataatgga
     2041 tgatcatttg ctgggcaaga aaatcattca gtgaatcttc tacaaaagga taagaaaaga
     2101 tcccgatccc aacgactaaa atgattgcca tgaagatgtc cagtattctt attctgcttt
     2161 tattcatcat gtccacctat tttctaatat gtatatgaag ag
//
LOCUS       DAJSNV010000187         2180 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSNV010000187 DAJSNV010000000
VERSION     DAJSNV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.193"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="ON516_002802"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="ON516_002802"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3346236.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DAJSNV010000188         2167 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSNV010000188 DAJSNV010000000
VERSION     DAJSNV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.194"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..132
                     /locus_tag="ON516_002803"
     CDS             <1..132
                     /locus_tag="ON516_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346237.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            285..1454
                     /locus_tag="ON516_002804"
     CDS             285..1454
                     /locus_tag="ON516_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3346238.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1721..>2167)
                     /locus_tag="ON516_002805"
     CDS             complement(1721..>2167)
                     /locus_tag="ON516_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346239.1"
                     /translation="NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDG
                     QVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVK
                     SESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          771 a          367 c          334 g          695 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 cttagtaaat ccattactat aagattaaaa taaatgttat caagatacaa gctattttag
      241 ctttattctt tctgtaaaaa agaaaggaga ctaaataaac tattatgaaa aaaggaaaac
      301 atgctaaaaa aagaaaatat tcatttttaa tgatcgttgt cgtgttgttt agccttagtg
      361 cgttctctac aggaactatt cttggatggc aattttacaa taatgaacac tctcagatag
      421 ttgcccatgc tcaatcagct caaaaaaagt tgatactgac taccaatgaa caacagctcg
      481 ttgaaaaaat cgatcaacta ttaagaaaaa atgattatat tggctcaatt tacgtaaaga
      541 aaaataatcg catcatcatg gaaaaaggct atggctatgc gaataaactc acgcaacaac
      601 caaatgatcc ttctctttat tatcagattg gttcggttca aaaagcattg actactcttt
      661 tgattttaaa acaagtcgag cgtgggcacc tttctttaga tactaagctg gctcaattct
      721 atccacaaat tgctggcagc cagcaaatta ctatccgtga cttgctgtat atgcgctcag
      781 gcttgcaccg aactgcttct ccaaccatac cgatgactga tgaagaagtc gttcaatttg
      841 cgattcagca cttaaaattt acagattatc agacctctca ttatgattca ttgaacttta
      901 ccatattgac tggaattttg atcaaattaa caaatcaacc gtatgaaaaa ttattgaccg
      961 atgatatcat cgaaccttta ggattacagc atacaaaatt ttatgaccaa gtcaaaaatt
     1021 catccaatca tgcactatcc tatcaaatgt ctaaagagga tgattacttt aaaccactga
     1081 cagaatctga aacagagatc cataacgaat tagggactgg taatatcagt atgtccgtct
     1141 atgatttaaa tacgctgttt acacaagttt tatctggaaa actagtttcc aaagacttac
     1201 tcttctcatt atggcgtgaa aatgcgaatg gacatccata tagtggtggg gtatatagcg
     1261 gagataatta tatcctgtcg caaggaaata tcgactcttt tgatacatct gctatattta
     1321 gcaaagattc acaggatgct atcatcatgg aaagcaatat aaaagctgac aaaactatca
     1381 aactgcctac tgctgatcta agaaaccaaa tctatgacct gatggaagga actactaagt
     1441 tggcaaatag ctgatatgat gaaaagatag caaatactac ttctttgaat agcatttgct
     1501 taaaaaaagt actcgactaa acgtaggaaa gagtgtggga caaatctaaa aatcaatttt
     1561 tgctacagac tttaattcca aataaacaga acagaaagag gtgttcttct ttctgttctg
     1621 tccaaattat tctaagattt ctctttacca acattatttg acaaagggcc tattaaatct
     1681 aaaatagtat ctttagattg ctaaaatatg tgctagcaaa tcattcatgg cttgttctta
     1741 tttttaattt tatgcaataa aaaagaagaa caattagcgt gattatagaa ataaataatg
     1801 agattttaga aaaaaatgtg tatgaatacc tgattgttac tttatttatt ccactttcac
     1861 tttttactgt aacaaaagga cctccttgtt ttagtactgt agcatttttt gaattaacag
     1921 taaccgttaa tcctttatac cggaatacag gtaaaactat tgttttgcct tttgaagatt
     1981 taaacgtaaa tgtaatttca tctttatttg gagaaaagct actataaata acttgaccat
     2041 cttctgtaac ttgatggttt ttcaaaagca attgatcttt tttatagcct ataggtacat
     2101 aatcaaaatt ctcaaattct tcaagtttat tttccatttg ttcattagaa taagttcctt
     2161 gaaaatt
//
LOCUS       DAJSNV010000189         2085 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSNV010000189 DAJSNV010000000
VERSION     DAJSNV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.195"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..132
                     /locus_tag="ON516_002806"
     CDS             <1..132
                     /locus_tag="ON516_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3346240.1"
                     /translation="ECDCGERAKCIKSYRLYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            132..383
                     /locus_tag="ON516_002807"
     CDS             132..383
                     /locus_tag="ON516_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346241.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLSPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            398..835
                     /locus_tag="ON516_002808"
     CDS             398..835
                     /locus_tag="ON516_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346242.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            828..1535
                     /locus_tag="ON516_002809"
     CDS             828..1535
                     /locus_tag="ON516_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346243.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            1916..>2085
                     /locus_tag="ON516_002810"
     CDS             1916..>2085
                     /locus_tag="ON516_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346244.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPAL"
BASE COUNT          776 a          327 c          447 g          535 t
ORIGIN      
        1 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca
       61 acatgcggga aaaaatatac cttgtcttat ggcgattaca ttttgattga tgagaaagga
      121 aagaaacgct gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt
      181 tggtggacaa agaagtcatt tctgaaaaag tagcggaagc tttacttagt ccgcggtact
      241 ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga
      301 agattgcagg atatgaactg cattatcaag aaaacatgga aacttatgaa atcgaatatt
      361 ggttaaccaa attagagaac taaaaaggag aaaaaatatg gctcaaaatt actatgacga
      421 atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga cgttcctata
      481 ccatgaaaca cgtgtaccca aaaaacatta caaggaaaaa ttgtcagtcg cagtggaaga
      541 aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg tactatcgaa cactggaaga
      601 actgaaaaaa caaaatggaa aatggttttt ccaagcctta ctatgcttgg aagtaggtgt
      661 caaaccaagc actatcaaac cttccgaata tcaagcctta gaactgactt atgcaaaatt
      721 cgcacagatt aagaaagcta aagcaatggc tagcgactgg ctagatactt ttgaaagtat
      781 cgaaaaaaat ggtgcatttt atacgattga atcggaggag aaagataatg aataaggtgg
      841 tttatcagct aacaacagaa gatgtttcgc aatttttaac aagcaaagaa cacttgaatg
      901 aagtaatcag acggattcaa aaagacttta gttgtatgga agaaatgaag tactttattc
      961 aaagagtgga agaacagttg tatatggaaa gcgaaacaag tcaaacgaag gtcacagtca
     1021 gccataatca tttagacgtg tataaacgtc gtcctgaaac ggtaaaaaaa ggaacagagc
     1081 ttctacgact agaaacagaa aaatacatca tgaatgtgta cctagaaagg agtgagctgg
     1141 tcgaaaactt tgtcagctac acgatttgta ggaaacacta ttactcagaa tcagaatatt
     1201 attcaatttt cgagagcatg gaattaatgg aagaagaaat ggaagagcta aacagcaaaa
     1261 atgcgacggt agacgattat taccttgtat ttactaggtt tttgacgtgt gggacggaat
     1321 ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc cgaagcattt ttgagggaat
     1381 atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact gattttttag
     1441 acacctacac ttacgacgat acagaagaaa tccaaaatga acttgaccgt aaacgaatgc
     1501 catatcaacg atttgaaagg aaaaaaggta aataaccatt tataccgaaa agtgaggatg
     1561 tgtgaaataa gaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg
     1621 ctccaaccgc ccccgccgct taggtggtgc gaaataagcg ggcaaagccc gataacctaa
     1681 gagaaagaag gagatccata tgaataaaat cattcctcta ttgttcgtac tgtttgttgg
     1741 cgagatactt atgcgtcatg ctgcagctgt acatttaaac ttcgtagtat tcttaggaat
     1801 actggtattt tgtttgatct tttggcgatt tgtattagcc cttgtgagtc tactattcaa
     1861 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagagag tactaatgcc
     1921 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     1981 gcagcaacta aagcagaagt acggaaaaag tgaatctaag attatgtgta ttgcgttaga
     2041 tctattgatc gcgcaagaaa aagcaggatt tgatatacca gcact
//
LOCUS       DAJSNV010000190         2084 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSNV010000190 DAJSNV010000000
VERSION     DAJSNV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.196"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            23..688
                     /locus_tag="ON516_002811"
     CDS             23..688
                     /locus_tag="ON516_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3346245.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(972..2057)
                     /gene="xerS"
                     /locus_tag="ON516_002812"
     CDS             complement(972..2057)
                     /gene="xerS"
                     /locus_tag="ON516_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3346246.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          667 a          318 c          356 g          743 t
ORIGIN      
        1 aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa
       61 gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat
      121 ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa
      181 agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt
      241 tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg gttttatttg
      301 tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa
      361 agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc
      421 caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga
      481 cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat
      541 gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat
      601 gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag
      661 tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa
      721 acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag
      781 agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac
      841 acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac
      901 acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt
      961 atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta
     1021 tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa
     1081 tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga
     1141 attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc
     1201 ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat
     1261 cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg
     1321 acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg
     1381 tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca
     1441 ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa
     1501 gtatttttat attcattggc aatataatct agataatttt gtgtatcctt atctagaaat
     1561 aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct
     1621 acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc
     1681 gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat
     1741 agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt tacattttct
     1801 tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt
     1861 atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc
     1921 ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct
     1981 tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat
     2041 atttctgtat ttttcatgtc attcatcctt tcaataagta gggg
//
LOCUS       DAJSNV010000191         2060 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSNV010000191 DAJSNV010000000
VERSION     DAJSNV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.197"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(71..226)
                     /locus_tag="ON516_002813"
     CDS             complement(71..226)
                     /locus_tag="ON516_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3346247.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            843..1064
                     /locus_tag="ON516_002814"
     CDS             843..1064
                     /locus_tag="ON516_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346248.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1065..1322
                     /locus_tag="ON516_002815"
     CDS             1065..1322
                     /locus_tag="ON516_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346249.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1458..2060)
                     /locus_tag="ON516_002816"
     CDS             complement(1458..2060)
                     /locus_tag="ON516_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3346250.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          465 c          313 g          603 t
ORIGIN      
        1 tcatgctata atcaccttaa ttgaacaaca gtctaacagc aaaagcagtg cgccaacact
       61 aatgcttttg ctattttttt tgctcatttt cctttccgtt agcattaacc caaattgtaa
      121 taagaccaga cttactaaga cccatttgtt tcgcaatttt atcaagatta tctgccactt
      181 ccttagtaag cgttacaacg actctctttt tttcattact gatcataatt cgtatcctcc
      241 aacgactttt tcttaaaaac catagtaaca aaaaagtaac taaggtgcaa cagtttagat
      301 actaattagc gaataatagg ccttaaaccc ttggcattac tgattttgtc aaaaaaaaac
      361 gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa tgaccaataa
      421 ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa ccaaaaaaca
      481 aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg caggcgccac
      541 gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt cgctaccagt
      601 cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc cgataactaa
      661 acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag cattgatttt
      721 ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt atattataag
      781 cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg gaaggagcat
      841 aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg accagcgaac
      901 ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa gaacatctga
      961 ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc ccagaagccc
     1021 ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct aaagaaacaa
     1081 ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat tttatcgaat
     1141 tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc acaaatattc
     1201 tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca ccttcctaca
     1261 ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct tatatttttt
     1321 gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta cctacttttg
     1381 gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca caaaaaaaga
     1441 gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc accatgacga
     1501 ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag cggaaaccaa
     1561 tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt gatattctta
     1621 gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact cgttactgac
     1681 aaataactag caatttctgc accaccatga gcgataatat cacttttagc atattgaaat
     1741 ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt agcacatttt
     1801 tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata cgtcttttta
     1861 ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc aggattcaca
     1921 tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa aaactccctc
     1981 caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt gcgttcatct
     2041 ttagtttttc tactcgtcat
//
LOCUS       DAJSNV010000192         2025 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSNV010000192 DAJSNV010000000
VERSION     DAJSNV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.198"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            639..971
                     /locus_tag="ON516_002817"
     CDS             639..971
                     /locus_tag="ON516_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3346251.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1590..1931
                     /locus_tag="ON516_002818"
     CDS             1590..1931
                     /locus_tag="ON516_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3346252.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          703 a          271 c          428 g          623 t
ORIGIN      
        1 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
       61 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
      121 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
      181 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
      241 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
      301 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
      361 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
      421 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
      481 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
      541 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
      601 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
      661 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
      721 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
      781 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
      841 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
      901 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
      961 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     1021 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     1081 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     1141 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     1201 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     1261 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     1321 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     1381 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     1441 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     1501 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa
     1561 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     1621 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     1681 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     1741 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     1801 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     1861 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     1921 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     1981 gattaataaa aaggtagtat taccttaggc attgagtaag gaatt
//
LOCUS       DAJSNV010000193         2024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSNV010000193 DAJSNV010000000
VERSION     DAJSNV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.199"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..660
                     /locus_tag="ON516_002819"
     CDS             <1..660
                     /locus_tag="ON516_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3346253.1"
                     /translation="VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
     gene            997..1236
                     /locus_tag="ON516_002820"
     CDS             997..1236
                     /locus_tag="ON516_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346254.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1598..1858)
                     /locus_tag="ON516_002821"
     CDS             complement(1598..1858)
                     /locus_tag="ON516_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346255.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          689 a          371 c          297 g          667 t
ORIGIN      
        1 gtatccaaag aagcgattta tattgccatt ggtatacgag aagacggcac taaagaagta
       61 ctgagttatg cgattgctcc aactgaatca acatacgttt ggaatgagct gctacaggat
      121 attaactcca gaggagttca agaagtcttg ctttttatta cggacggctt aaaaggcatg
      181 aaagatacta tccatcaaat ttatcctaaa gcaaaatatc agcattgttg tatccatgta
      241 tctcgtaaca tcgctcataa agtacgtgtc aaagaccgaa aagaaatctg tgatgacttt
      301 aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata ccttcttatc cggcatgatt
      361 gagaaatgga agaaaaacta tcctaaagtg acgcagtcac tcatagaaaa ccaagactta
      421 ttaacttttt atgattttcc acctagcatt cgtagaacca tttactcaac caatctaatc
      481 gagtctttca ataagcaaat taaaagatac agccgtagaa aagagcagtt tcaaaatgaa
      541 gaatcactag aacgctttct agtcagcatt tttgatacat acaatcaaaa atttctaaac
      601 agaagccata aaggttttca acaggtaacc gatacattag tttcaatgtt tactgagtaa
      661 ctaattattt tgcaggagga caatttattt acacaaaatt attgacgctc ccagctatcc
      721 tcctagcaac tatttaaata cccaaaaaaa ttacctctag ataatatcat acctacgttt
      781 tcacttataa attttttcat aaaaaaagcg tttagaggca taatcgttaa tacagatggt
      841 aaccaatgta tctggtttga actgggatat caccctctcc atatatgtat tactaagact
      901 aatagatact ataaaagact tgatagtttt gcattgattt tgcctaatac tgagcatagg
      961 tgtatgctaa acgatactat aaggagtgtg tacataatga aaagaattct gtttatcagt
     1021 ttatcaaatt tattgttact tactgcttgt gggacttctg catcaaagaa cataccttca
     1081 gatacgcaaa tttcgaatag tatcgattca acaatttcta caaatgagaa aaattctccg
     1141 gtagaaactt ccgatataaa agtttctacc tctgcaaaaa acagggtaaa gaaactgtca
     1201 gcacgtctcc tttatttcaa acaaactctg aattagcttc ttcatcttct tcagcaaata
     1261 atgatgatag tttcgcgttt actcctaact aaatgcaacc tttacaaatg ctttacgata
     1321 acaagcctga gctaaatgat cctgatatca gttttgcatt ttttgaaatg attggatcgg
     1381 atgatctttt taaaactact agtttgtcta tccgaaaata ggacagttct ggcagtatag
     1441 gtttttatcg agtttctcca ccaggcctcg ttacagagac agattctcta ggcgaacctt
     1501 ttgaataaat caaaaatcat gaacaatcac tgccaactaa ataaagtgcc ttcaaaagct
     1561 tggtcttctc gcttttgaag gcactttatt taaatcttta tgaaactaaa gtatgttcat
     1621 atttgttgct atagttatat agatattctg ttgtttccaa ctcataaacg tgagtatatt
     1681 ttgccactac ttttccgcat ttttctaata tttcattgcg atcgacgtca tgatattcgt
     1741 ctacgcagat cacaacacaa ttttctaatt tactgattac ttctccaacc actttttgtt
     1801 tcaagccaat aggttggcaa atatattttt ttcctaaaat gatagtttcg ttattcatat
     1861 ttcatccatc cttaatgttt ttccattatt taaaatgata ataccattaa aatatgatta
     1921 tgaaaatcaa taacacttgt gattttataa aaaaataaaa aaaccgaaaa acagaaatgc
     1981 ccactgtctt tccggctctt tttattcttg atttctcttc tttt
//
LOCUS       DAJSNV010000194         2008 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSNV010000194 DAJSNV010000000
VERSION     DAJSNV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.200"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            62..1255
                     /locus_tag="ON516_002822"
     CDS             62..1255
                     /locus_tag="ON516_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3346256.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1421..1843)
                     /locus_tag="ON516_002823"
     CDS             complement(1421..1843)
                     /locus_tag="ON516_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346257.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          533 a          337 c          408 g          730 t
ORIGIN      
        1 ttattctttt ttggatatac ttttaacaat ttagggaaat tttaggaaaa ggggtataaa
       61 aatgacaacg tatcagcgaa agttattatt atcgacatcg gcgggaattg ctttagagaa
      121 tatggatatt atgtttttag ctttttcatt atcttcaatg attacttctt ttcatattag
      181 tggtacgcaa gcgggtttca tttcaacgat cactaatttg gggatgctca ttggaggtat
      241 tttttttggc atcatagctg ataaatatgg acgtgtgaaa gtattttctc aaacggtcct
      301 tctgttttcg attgcttctt tattgatgta ttttgcttca aacatctact tggtttatct
      361 ctttcgcttt atcgcaggtg taggagcggg aggagaatac ggggcgtgta tgtccttgat
      421 ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct tcggttgctg gtattggtgc
      481 gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg atcattccaa cgtttggttg
      541 gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg
      601 cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag
      661 cagactgttt gaatcaaaac atcttgcttg gcaaacatgt ggccttagta tcatggtaac
      721 tgtccaaatt gccggctatt ttgggttaat gaattggctg ccttcaatta tgcaaaatcg
      781 attagggctt tcggtttctg ggtcttcatt gtggatggtc agtactattt taggcatgtc
      841 tttggggatg atgacttttg gcataatcat ggataaattg gggcctaaac tagccttttc
      901 tttgttttta atttgttcat ccttatctgt ctatctactt gtgttagccc atagccagtg
      961 gagtttggta gcagccgctg tgatagtcgg ctactttatc aatggcatgt atggtggata
     1021 tggcgcagtg attagtagtt tgtatccaac agaaatccgc gcgacggcga ataatttcat
     1081 tatgaacctt ggacgagctg ttggtggttt ttcttctatc gtcatcggtt tcttgatgga
     1141 tcattatagt ttaactgccg taatcttgtt tttgagcggt atttacctag tcagtctgct
     1201 ggtcttgaca actcttactg gtgttacagc attaagggaa agcttgcagc attgatagtt
     1261 gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg catctgcggt aataagacca
     1321 gccaaatcaa aaacatgaga cactgtttcc gtttggctgg tcgtattact tgatgcaggg
     1381 cagctttttc aactgctaat ttccttctat ctggcggcta ctattctttg acgtccgtca
     1441 tttcaaagta ctttactaat aacttagcat aacgaaaaac tgtggataca ctcgtatatt
     1501 gagcgtctgc ataatcttcc aaatttttct caggatgttg atacatgaat tcaatcaaat
     1561 gataattata tgattttctg tagaaatgaa gataaataat gtagattggg aagttctcct
     1621 gcttttccat agaaaagcgc cgattattct cattcttcaa aatgacctta tcaggatatt
     1681 tttggaacaa ttcttctaaa tctgttagat aattcaagat agacttactt gataccttca
     1741 attcgttttg tagttcttct attgaacacc aacgttctgt ttcaagcagt aaatgaatca
     1801 ttttaatttt cttttgagaa acttttgtta aaaaagtctg catattttct cctcctgtat
     1861 ctactttact atactacata caagaaaaaa aacggataca aatatcatat tgtcacaccg
     1921 tcaatagtgc ataaacctgt tattatttct ctttttttcc taagtattct attgatatga
     1981 atcatttaat cgtttattat ataaatgt
//
LOCUS       DAJSNV010000195         1993 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSNV010000195 DAJSNV010000000
VERSION     DAJSNV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.201"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            74..403
                     /locus_tag="ON516_002824"
     CDS             74..403
                     /locus_tag="ON516_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3346258.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="ON516_002825"
     CDS             393..680
                     /locus_tag="ON516_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3346259.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            964..1821
                     /locus_tag="ON516_002826"
     CDS             964..1821
                     /locus_tag="ON516_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3346260.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1822..>1993
                     /locus_tag="ON516_002827"
     CDS             1822..>1993
                     /locus_tag="ON516_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346261.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          741 a          266 c          393 g          593 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttat
      721 cgaaaatctg attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt
      781 aaataacgtc attactttga ggaaagctat aaatcaatat atttcagctt ttccttattt
      841 atgtactacc ttttacttat taattataag gtagtacata tatatctttt tttggtagta
      901 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
      961 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
     1021 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
     1081 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
     1141 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
     1201 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
     1261 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
     1321 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
     1381 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
     1441 gcggattggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
     1501 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
     1561 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
     1621 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
     1681 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
     1741 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
     1801 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac
     1861 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1921 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1981 gttgaaagac aga
//
LOCUS       DAJSNV010000196         1954 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSNV010000196 DAJSNV010000000
VERSION     DAJSNV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.202"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ON516_002828"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ON516_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3346262.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DAJSNV010000197         1933 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSNV010000197 DAJSNV010000000
VERSION     DAJSNV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.203"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(856..1386)
                     /locus_tag="ON516_002829"
     CDS             complement(856..1386)
                     /locus_tag="ON516_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3346263.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1383..>1933)
                     /locus_tag="ON516_002830"
     CDS             complement(1383..>1933)
                     /locus_tag="ON516_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3346264.1"
                     /translation="VLLTILGLVYFMYATDGIAAGEGYKRILMELYLHLKKDRKYRKV
                     CALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIYKYNTTTGTKELSTACLENIILFI
                     PYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFVYNT
                     LGGILGVILYKLILITKRDGER"
BASE COUNT          622 a          377 c          220 g          714 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cag
//
LOCUS       DAJSNV010000198         1875 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSNV010000198 DAJSNV010000000
VERSION     DAJSNV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.204"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            49..642
                     /locus_tag="ON516_002831"
     CDS             49..642
                     /locus_tag="ON516_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3346265.1"
                     /translation="MIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            708..>1875
                     /locus_tag="ON516_002832"
     CDS             708..>1875
                     /locus_tag="ON516_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346266.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKE"
BASE COUNT          802 a          231 c          324 g          518 t
ORIGIN      
        1 gttaaagtta acgatgaaaa agaacagcaa aaagcaataa aagaacaaat gattgaagaa
       61 gaaaaggtaa gacgtgaaat agaacgtgaa aagaaacgca ttgataaaga agaacgacag
      121 tttaactcag agataactaa gttaatggca tacatgcaaa aagctagtgc tgatgttgaa
      181 aaagaactct atgctgataa aattaaagaa ctggaagaga aattaagaga actagaaact
      241 gtcaaagaag atgtattgca aagagaactt aatactagag ctggctatgt ttatgtcatc
      301 agtaatatcg gatctttcgg tgaagatgtc tataaaatag gaatgactag acggttagaa
      361 cctatggata gaattaagga acttagtagc gcttctgtac cttttgaatt tgatgttcat
      421 gcaatgatat tttcagaaga tgcaccaaca ttagaaaatc aactacattc gcattttaga
      481 aaaaacgaag tcaataaagt taatcaacga aaagaattct ttaaagtatc tcttgatgaa
      541 attgaaaaag ttgttttaga aaattataac ggaactgtat cttttactaa actagctcaa
      601 gctgaacaat acagaagatc attagaatta acagatgtgt agtaaaaaga ttagccttcg
      661 ggcttttctt ttacaacgat aagaacatac attcgaaagg agataacatg aaacgagtag
      721 cattatatat gagagtatca actgaacaac aagccaaaca tggagatagt cttagagaac
      781 aaaaagaaac cctctatgaa tatatcgaac aacacaagga tttgaaagta gttaatgaat
      841 atgtagacgg tggtatttct ggtcaaaaaa taaatcgtga tgaatttcaa aaactattac
      901 aagatgtaaa agaaaataaa atagatctaa ttttatttac aaaattagat agatggttta
      961 gaaatctacg acattattta aacactcaag aaattttaga aaaacacaat gtttcttgga
     1021 atgctgtttc tcaacaatat tatgatacaa ctacagcgta tggcagaact ttcattgcac
     1081 aagtaatgag ttttgctgaa ttggaagccc aaattgattc agaacgcatc aaagctgtta
     1141 tggcaaataa aattgcacaa ggtgaagtag taagcgggaa aactccgtta ggctactcta
     1201 tagaaaataa gaaattagta ataaatgatg atgctcctat cgtgatagat atattcaact
     1261 actttctttc tagtggtagt ttaagaaaaa ctgtttatta tttaggctct cagtatggaa
     1321 tcgttagaga ttatcaaagt gttaaaaata tgttgacgaa caaaaaatac attggtgaat
     1381 taagaaataa taaaaattac tgtcctccta ttattgataa aaaacttttt tatgctgttc
     1441 aaaaagcctt acctaaaaat ttaaaaacaa atgcaaaacg tgattacatt tttaaaggtt
     1501 tattaaaatg ttctgactgt cagggatcag tagctggtca aactattaaa gcaagatata
     1561 agaaaaaaga cggtacagaa tctatatacg aaaggacttg ttatagatgt gtaaaacgaa
     1621 gaaataataa attacgttgc acaaataagc gcgcttttta cgaaaaaaat ttagaacgtt
     1681 atatttttga agctactaaa caaaaatttg aacaaataca aataaactat tcaaaaaaac
     1741 aaccaaagat tattaaaaag aaaaatagta agaaaaatat tgagaataaa ttagatagat
     1801 tgaagaaagc ctacttgaat gaagttatag acttagagga atacaaaaaa gatcgagaag
     1861 ctttaatgaa agaat
//
LOCUS       DAJSNV010000199         1874 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSNV010000199 DAJSNV010000000
VERSION     DAJSNV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.205"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(592..882)
                     /locus_tag="ON516_002833"
     CDS             complement(592..882)
                     /locus_tag="ON516_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346267.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1090..1782)
                     /locus_tag="ON516_002834"
     CDS             complement(1090..1782)
                     /locus_tag="ON516_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3346268.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          502 a          362 c          293 g          717 t
ORIGIN      
        1 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
       61 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      121 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      181 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      241 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      301 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      361 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      421 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      481 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      541 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      601 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      661 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      721 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      781 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      841 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      901 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
      961 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1021 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1081 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1141 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1201 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt
     1261 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
     1321 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
     1381 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
     1441 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
     1501 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
     1561 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt
     1621 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
     1681 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
     1741 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
     1801 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
     1861 acatgtgtaa tttt
//
LOCUS       DAJSNV010000200         1780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSNV010000200 DAJSNV010000000
VERSION     DAJSNV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.206"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            246..1724
                     /locus_tag="ON516_002835"
     CDS             246..1724
                     /locus_tag="ON516_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346269.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          647 a          246 c          312 g          575 t
ORIGIN      
        1 cttttttgtg gttgtttacg caaatggcgc aaatcttgta cattttaaca caataaaaca
       61 aaaaatatag aatatttcct cataaatttt tccatttttt gcaatattct caaaaaaaag
      121 ataaaaaatg agaataactg atgaaaaaac aagcataatc catcataaaa tcagtatagg
      181 tgaaaagatg aactttcatc tgcatatcaa gtaattggta tgaggcattt tttgaggtgt
      241 aggatatgaa gttggattac cttatagaag aggaagatag agaaaaactg ctgctagcta
      301 gatatttaga atttgagaaa aagaattatg tagtgctgcc taagttatat gaaacattag
      361 gtttgtctaa atttaaaaca agaaattata tcatagaatt aaataaagaa tttactgaat
      421 tcaaaacaaa gccagagatc caaatttcag aagaagcaga gatccatgtc gaacgtctga
      481 atttgacgga tatcaaaaat ttaagaagaa tctactttca acgatcaaaa attgctcgac
      541 tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa atttgcagag aatcattttt
      601 taagcatatc aagagcctat gtaaaaagaa gagaattagt ctcttttctt gaacagcatc
      661 aaataaagtt aaagaaaaac atattagttg gggaagaaat aaacatacgg aacgtattat
      721 ttggggtata tttcggggtc tttaatggtt tttctatgcc atttagagaa agcgtcatga
      781 atgaagcaaa gagtatcgtg caatattttg aatatctgtt caatttaaaa ctgtctgcaa
      841 caaaacgaat caaattcaat ttgctaacca ctattagttt gttgagggcc tatgggaatc
      901 aaccaatcgg aaaacgtttc ttttcagatg aatactttaa gagctcagaa ggaatgaaga
      961 tcatcaagga tttatcacat atttcttttc ttcatatccc taaagagcag atcattgatg
     1021 agatttctta tatcatgttg tttttgatca cggaagaaga atttgaatta gatggcgcat
     1081 ttggacttgc ggaagctcat tttcatgata tccacatgat tagtcaaaaa atgtcagtgg
     1141 aattgattga tcaattacca ttttcgattg aaatctctga agaagaaaga ctatatatac
     1201 gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa aaattggata ttcgactttg
     1261 agatgactag ctttacgtct aaaaagcagc tacagttttt cttcgagacg tatccaatat
     1321 tcagtacagc aatccaaaag gtcatacaag agaataaacg ctatttcttg ggaaaagatg
     1381 aacgtttcat gattcgctta ttttatgatt atctcttttt attggtcggt atttgtccgg
     1441 tatcatattt tgaagcaccg attcatgtct gtattgattt ttcgcaagga tctgcttata
     1501 ccgattacat tatttctcaa gtgaaaggat ttaaaaatta caatattgtg attgagagtc
     1561 gaatcacttc tcgtacgaat atttatttat ctgattgttt acttgaaaaa tttaataaag
     1621 aacagataat ttggaaaaat ccaccgactc cagatgactg ggaatatttt ggaaatacca
     1681 tcatcagaga aaaaaataga catttaactg aaaaaatcag ctagtattta tggatggcag
     1741 ctttgtctca catatttatt agctgattta tgtttttttc
//
LOCUS       DAJSNV010000201         1778 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSNV010000201 DAJSNV010000000
VERSION     DAJSNV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.207"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..196)
                     /locus_tag="ON516_002836"
     rRNA            complement(<1..196)
                     /locus_tag="ON516_002836"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(870..1226)
                     /locus_tag="ON516_002837"
     CDS             complement(870..1226)
                     /locus_tag="ON516_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3346270.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1240..1650)
                     /gene="spx"
                     /locus_tag="ON516_002838"
     CDS             complement(1240..1650)
                     /gene="spx"
                     /locus_tag="ON516_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3346271.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          529 a          338 c          272 g          639 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tacgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aaatgtttgt tgtcttgaat tgaatcaaga cgccctacac
      301 atttggtttg tcttactttg ttcagttttc aaaggtctac ttcatcagga gtgttccccg
      361 attcgttttt ggctgccgta gcaactttta tatcatatca gagagacaag taattgtcaa
      421 ctctttttta aaagtttttt tgatttcgtt tttcgaaatc ttacagcaac tcatttatca
      481 taacttatca tttgttattt gtcaagaact ttttttgaag tttttttcga tagaatcaag
      541 cttcacaagt ggttcttttt taacaactca gttatcttat catgtcattt ctaacatgtc
      601 aacaactatt ttatatgttt tttaaaatac tattttattg ctttgctact taaaacaaca
      661 gaaatgaata tatcatgtaa atttacacat gtcaatatat ttttaactct atatccccac
      721 ctagatttga attttgacca cataaattga gcatgtctct atagtagaaa aataaagtca
      781 aaaaaagaac acataacacg cttgatgcta agtgttcgac tgttacaact gtatgaaatg
      841 ctcagtgact tgattaaaac agttaaagtc tatttattta agataagctg ttccatcaac
      901 tgctcattgt atccaataaa tagaacttca tcgataataa tcaaaggacg tctgaggaga
      961 ttggtatgtt gtgtgcataa aaaaatcagt tgtttttttc ctacacagga aaggtcaatc
     1021 gcaatgttgt gaaaagcctc agaccaagtc gcaaatagct gttctgcttg cacttcttca
     1081 aaactgcata attcttctgc ttcttctgta cttagatcgc gttcttctaa gtcaacaaat
     1141 tgataaggca attgatggtg ttctaaccat tgtttcgcct tcagacttgt tacacagtgc
     1201 gctgatccat agactttcac ttccacgaag gctccctccc tatgaattta agatgttatt
     1261 gttttgtaaa tagatcagat ttctggcaac acatcgaatt tcacgaggga caaatgaccg
     1321 gatctcatct tcattaaatc cgattagtat tcttttttca tcaatcaaaa taggtcgtct
     1381 caataatgaa ggatattctt tgaataagac gatcatttgc tttaaagtta attcgttaaa
     1441 atctatgccc agcttttcgt aaattttcga ccgagtggaa ataatatctt gaactcctcc
     1501 ttcagtcgct gccaagatct ttttgatctc actttctgtt aacggttcgg caaaaatatt
     1561 cttctcttca aattcaagtt gattcgcttg caaccatgct cttgcttttc tacaggaagt
     1621 acagctaggc gaagtatata cttttatcat tttctcactc ctttttaatt ataatccaat
     1681 tctacagtaa tttcttttaa aataccaatt attagctaat aaaatataaa aaaaaatcaa
     1741 aataactgat tatttacgtt tgaataatga gaattttt
//
LOCUS       DAJSNV010000202         1773 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSNV010000202 DAJSNV010000000
VERSION     DAJSNV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.208"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            23..1357
                     /locus_tag="ON516_002839"
     CDS             23..1357
                     /locus_tag="ON516_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346272.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          612 a          255 c          308 g          598 t
ORIGIN      
        1 tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt tatatacttt
       61 tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag caatcgctct
      121 tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata gagaaaaaaa
      181 agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct taactacact
      241 attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa gcaagcaaat
      301 aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt tgacttgtgc
      361 attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag aaatacacag
      421 tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta tcattcaagc
      481 gttaaataac gatattacaa catacactat ggggttatta ggattggttg cgttgatttt
      541 tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg ttggcgtagt
      601 ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa aaacaccgat
      661 aagcttactc cagtttcctt ggcgtatctt aggtatccaa atcttttttt cttcaatgat
      721 cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt taatcattgg
      781 cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt acgcaaaagt
      841 tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac gttatacaac
      901 atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag aaaggcctca
      961 cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt ataaagctgc
     1021 agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga cagtaccagt
     1081 atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg actcttataa
     1141 aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag aaattacaac
     1201 aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt attgtttagt
     1261 ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc gatatgtgac
     1321 taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata ttgagtggat
     1381 taattatagt tagtagtata tattctttca tctttttcaa aaatacacag agtatttata
     1441 ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact atttttacca
     1501 gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat ttttatccct
     1561 ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg attgtaagct
     1621 atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat cattatggcg
     1681 agcggttttt aaaacaaaaa aaagcggcat ttttatttta agtagttcag ttttggagta
     1741 caaaatgaca aaatgtactt caaaatggac tac
//
LOCUS       DAJSNV010000203         1747 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSNV010000203 DAJSNV010000000
VERSION     DAJSNV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.209"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..182)
                     /locus_tag="ON516_002840"
     CDS             complement(<1..182)
                     /locus_tag="ON516_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3346273.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ON516_002841"
     CDS             517..951
                     /locus_tag="ON516_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3346274.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ON516_002842"
     CDS             974..1294
                     /locus_tag="ON516_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346275.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ON516_002843"
     CDS             1435..1626
                     /locus_tag="ON516_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346276.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          666 a          261 c          364 g          456 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattac
//
LOCUS       DAJSNV010000204         1718 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSNV010000204 DAJSNV010000000
VERSION     DAJSNV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.210"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..891)
                     /locus_tag="ON516_002844"
     CDS             complement(<1..891)
                     /locus_tag="ON516_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR3346277.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFK"
     gene            complement(1050..1316)
                     /locus_tag="ON516_002845"
     CDS             complement(1050..1316)
                     /locus_tag="ON516_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346278.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1306..1662)
                     /locus_tag="ON516_002846"
     CDS             complement(1306..1662)
                     /locus_tag="ON516_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3346279.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          496 a          328 c          207 g          687 t
ORIGIN      
        1 tttaaatttc ttatagtcct ctgaaatcaa aatatagcgt gatggatact catcaaaaga
       61 agaatcgtat tcttccttta aaattcccga tttacttaat ttttctagta catcaaatac
      121 tctatcactc aagtaactcc ttaaattgtt atcactttcc cattgaacta cttttctata
      181 agttgaatta tcagttgaaa agatagaatt ttcatccata aacgcatata ttagcagcaa
      241 tctttcatca ttgtcaaatt ctattggatc aaaaatatct aagttgtcat caaaatcttt
      301 atcagttccg gtaacttttt tgttaattac tgaaggtgtc ttgtaagaaa tagaatctaa
      361 atcaaagcgt tttgattcta aattattttg tgctttctca attaaataat tcactttttt
      421 tgtgagaaac cttctgatat tgtctttgct atatgttcca taaaaaccct tatcttctaa
      481 agagtccctg tacttcttaa cttctatata ctcttcagcc aaaacttgtg gaactggttc
      541 atctaagaaa aaaaccccta cctcgccttt gctattttta taaaagtaat caatttctgc
      601 tatagttccc gatctataat tatcaactgg agaacccaac ttatggccaa aaatcgctaa
      661 taatatatca ctattttcta caatttgttt attaataaca ctttgcccag agctattatt
      721 tatataagta ggaatcgcat tcgtttccca tcgaattggc atcaaaatta cttttgtatt
      781 atctgtattt tctatattcc atctatatat ggtttcttct acttcattcc tctttgctgc
      841 tatatcacct ggtgatgcta taaatacttt tattagattt atggttttca tttattgtac
      901 ctttctcttt atgtctcttt aaagtaattt ttgtataaaa tttattgact tttacactat
      961 aggacaactt ttatctattt agtacaaaaa ggcccactaa actaattgat tagttaggag
     1021 cctattacag gtatattttc aactcgcttt taagcaaatt ctactaccag ctttttgcct
     1081 aatgccctag cgatatcatc aagtttatcg aaagatacgt tatcccctct ctcaattcga
     1141 gcaatagtag attgagttgt atgtgctttt tgtgcaagct ccgcttgagt taggcctgct
     1201 tcctcccgtg catgataaag agcaaccgca gtttcgagtt ttcttccttc acggtcatat
     1261 gcttctgcaa aatctttgtc tttttgcttt tcttcaatca attttttaat catcatttgt
     1321 gaaccactcc tctctaagtt cttttgcatg tttaagttca cttattggtg ttttctccgt
     1381 ttttttagta aatccatggg taatgacata ccttccattg accacatgga aatagatagc
     1441 tctttggatg tttgatccta ccttacttct taattcgtac aaattagtgt ctaatttttt
     1501 cacccatttc aaccgttgag ctactagcat cccattcttt tctgtttctt ctattgtacg
     1561 taaaagcttc gcactatctt tttttggtaa tgtttccaac cattctaaaa attcatcatg
     1621 accattaggt cttttaaatg tttcaaaaga tattttttcc atacccaatt atatcatata
     1681 ttgattttat attatagtat ataacataaa aattcgaa
//
LOCUS       DAJSNV010000205         1711 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSNV010000205 DAJSNV010000000
VERSION     DAJSNV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.211"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(148..525)
                     /locus_tag="ON516_002847"
     CDS             complement(148..525)
                     /locus_tag="ON516_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346280.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(825..1706)
                     /gene="galU"
                     /locus_tag="ON516_002848"
     CDS             complement(825..1706)
                     /gene="galU"
                     /locus_tag="ON516_002848"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3346281.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          479 a          380 c          252 g          600 t
ORIGIN      
        1 gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa
       61 aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta
      121 tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc
      181 gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt
      241 tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac
      301 gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc
      361 tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa
      421 tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt
      481 gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct
      541 ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc
      601 atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcag
      661 aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac
      721 tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt
      781 aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt
      841 agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga
      901 agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt
      961 cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt
     1021 agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga
     1081 gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt
     1141 tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt
     1201 ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt
     1261 tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc
     1321 cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc
     1381 cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca
     1441 actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt
     1501 gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg
     1561 attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga
     1621 agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca
     1681 ggaatgactg cttttttgac cttcacttgc t
//
LOCUS       DAJSNV010000206         1670 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSNV010000206 DAJSNV010000000
VERSION     DAJSNV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.212"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            53..1081
                     /locus_tag="ON516_002849"
     CDS             53..1081
                     /locus_tag="ON516_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3346282.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1137..1355
                     /locus_tag="ON516_002850"
     CDS             1137..1355
                     /locus_tag="ON516_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346283.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1426..>1670
                     /locus_tag="ON516_002851"
     CDS             1426..>1670
                     /locus_tag="ON516_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346284.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVV"
BASE COUNT          628 a          191 c          317 g          534 t
ORIGIN      
        1 tttttggatt caaatgatga aatattatat ttaaataagg agtatgataa taatgaatag
       61 caaaaaactt cagatgggtg aaattgttag aggtcctagt caaatatcta ttgatgttac
      121 atataaatgt aattttagat gtaaacattg ttacaatgcg agtggtggag acaattgttt
      181 tatggataat gagttgactg atgatgaact gttagaactt acaaaagata tatgtaattt
      241 aaagccatat aatatttgtt tctgtggagg agaaacttta ctgagaaaaa aaatagtttt
      301 atcttgtgcc actttattaa aagaggcagg tattcctaat attgctattg ttactaatgg
      361 atcattagta actcaggatg ttgcaaaaga attattcaaa gcaggtatta atcgatttca
      421 agtaagttta gatggaaaaa atgctagtag ccatgaagca cttagattat atccaaacag
      481 ttttgaaaag gcaattaatg ctatcaaatt atttagagaa ttgtccgaag aagtggatgt
      541 tgctttttgt cctactagtt ttaattacaa tcaattacga gaagtatatc aactagtaaa
      601 tactcttaaa gtgaagtctt taagaattca accatttatg cttagtggta ggggaatgca
      661 aaatagggaa atagtgccaa ctgtttctca atatagaaaa ctagtttctg atattaacta
      721 tattaaaaga aatgaagatt ataccaagat agattgggga gatccaattg atcatatcat
      781 ccgacacaga actttattga aagatttttc gaattttgtt tctattaggt cagacggagg
      841 attaatggca tcaccttact tgccaattca agttggaaat attaaatgtc acagtctttc
      901 taaatattgg cgagcaggac tttataaagt tttttcttta gatttagttc aagatatagc
      961 tactcaagtt tcttcaatac aagatatgag taaagaatta aaaaaaggac atattaaatg
     1021 gcttaatgaa gatatagaaa ttgatattct cgatgaatac aaacatgata ttttaattta
     1081 ggtttatcat tggaaagttt agataaacaa agtactttta gaaaggaggg aaaaaaatga
     1141 tttctgaacc tcaaatgaat aatatgtatg aaagcccaac tataatggga gctggtgaag
     1201 ctgcgccaag agcttgttca gcaattatgg tgtgtgtgtt attagcatct gcaggagtag
     1261 gtatccaagc aggagcggtg tatacttatg ctgtagttgc tacagtagct gtagctgcag
     1321 ccgcagtttg gaaaacagta ggattagttg gttaggaatt aataggagac taactgtctc
     1381 ctattgattc caatgattaa gctaaggagg aattttaaga aaataatgaa atacaataat
     1441 ttaaaaccga aattacttaa tgagaacata gaatatataa gagaagaaaa ttatggatat
     1501 ttaaccatag ttcccaaaaa tggaactgaa ttgaaagata acgttttcaa tccaacagta
     1561 agatatatat tggctaactg tactggaaaa aacagtgtaa aaaaaatagc gaatggttta
     1621 aaaaagaagt ttcctagttt agaatatgaa aaattctatc aagatgttgt
//
LOCUS       DAJSNV010000207         1664 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSNV010000207 DAJSNV010000000
VERSION     DAJSNV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.213"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            131..505
                     /locus_tag="ON516_002852"
     CDS             131..505
                     /locus_tag="ON516_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3346285.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ON516_002853"
     CDS             complement(619..1248)
                     /locus_tag="ON516_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3346286.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ON516_002854"
     tRNA            complement(1382..1454)
                     /locus_tag="ON516_002854"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAJSNV010000208         1647 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSNV010000208 DAJSNV010000000
VERSION     DAJSNV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.214"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(20..91)
                     /locus_tag="ON516_002855"
     tRNA            complement(20..91)
                     /locus_tag="ON516_002855"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ON516_002856"
     tRNA            complement(113..200)
                     /locus_tag="ON516_002856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ON516_002857"
     tRNA            complement(225..298)
                     /locus_tag="ON516_002857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ON516_002858"
     tRNA            complement(312..382)
                     /locus_tag="ON516_002858"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ON516_002859"
     tRNA            complement(384..459)
                     /locus_tag="ON516_002859"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ON516_002860"
     tRNA            complement(478..551)
                     /locus_tag="ON516_002860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ON516_002861"
     tRNA            complement(564..653)
                     /locus_tag="ON516_002861"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ON516_002862"
     tRNA            complement(677..750)
                     /locus_tag="ON516_002862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ON516_002863"
     tRNA            complement(764..837)
                     /locus_tag="ON516_002863"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ON516_002864"
     tRNA            complement(865..937)
                     /locus_tag="ON516_002864"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ON516_002865"
     tRNA            complement(957..1030)
                     /locus_tag="ON516_002865"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ON516_002866"
     tRNA            complement(1040..1113)
                     /locus_tag="ON516_002866"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ON516_002867"
     tRNA            complement(1124..1209)
                     /locus_tag="ON516_002867"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ON516_002868"
     tRNA            complement(1226..1297)
                     /locus_tag="ON516_002868"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ON516_002869"
     tRNA            complement(1309..1381)
                     /locus_tag="ON516_002869"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ON516_002870"
     tRNA            complement(1399..1480)
                     /locus_tag="ON516_002870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ON516_002871"
     tRNA            complement(1488..1560)
                     /locus_tag="ON516_002871"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ON516_002872"
     tRNA            complement(1564..1636)
                     /locus_tag="ON516_002872"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAJSNV010000209         1647 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSNV010000209 DAJSNV010000000
VERSION     DAJSNV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.215"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..216
                     /locus_tag="ON516_002873"
     CDS             <1..216
                     /locus_tag="ON516_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3346287.1"
                     /translation="FKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLE
                     NYNDLKSTNNYQFGNLSYEMISKVLSL"
     gene            223..735
                     /locus_tag="ON516_002874"
     CDS             223..735
                     /locus_tag="ON516_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346288.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1006..1629
                     /locus_tag="ON516_002875"
     CDS             1006..1629
                     /locus_tag="ON516_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346289.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          646 a          198 c          276 g          527 t
ORIGIN      
        1 tttaagttag cactttatgc acaatttttt attgaaaaat taaataggtt tcaagaaata
       61 gttgaagata gtgattttaa cacttttaat agccataaac cgatgagagg tacttatata
      121 aaatttctcg aaaattataa tgatttaaaa tctaccaata attatcaatt tggaaacttg
      181 tcttatgaga tgataagtaa agttctaagt ttgtgatgat aaatgaaatt aaatagtata
      241 aatgatttat ttgctattat aggtgttatt gctaacatac ttttttcgtg tttgaatttt
      301 agatttactc aaaagaacaa aaagaacaaa ctcagtatag aacaaatgaa aaaagacatt
      361 gaaactgatt taaaaaatga ccataaagaa agaagaaaaa ttgaagaata tacttcttta
      421 atggaaaagt gtaaagcaac tgatgcaatt tttagctttt taagaaaaag tgatgagata
      481 atcacacgtg aaaatataga tttagaaaat gagattagta aacttgaatc tatcttttcc
      541 gatataaatt ctcttaagta tttaatcgtc gaagaaaatt cggatagctt tttagaattt
      601 gaaaaatatc taacagatct tatagataat agaaataatg aattaatgag acaaaacatt
      661 gcaagattcc ttactaaatt agtttctgaa tggaaaagta ttatttctca aaatatggat
      721 aaattaagat attaaggatg ttagttttaa aataaactaa tgtccttatt tttgtgttca
      781 catttctgtt acctaagaaa tacttaaagg aggtgatgta gccagcaacg actacaactt
      841 gaacccgcta tctacagttt atttgttgtt tcttttgggt atactctaat ggtacaaatc
      901 catatttgct taaagcgttg atttttcagc ggtttttctt tattgcaaag tgtaccctaa
      961 gacctgtata ctaatagtaa atagattgga taggaatgat ggcaaatgta tttagcttat
     1021 ttaagagttt cttccgacga tcaaagttta gctcggcaac ataagctaat tgaaaattgg
     1081 tcagtcaaaa ataatattgc tgaagacgat ttaaaaattt tcgaggaaaa agtctctgga
     1141 aaaaatattg aaaacagacc agagctgcaa gagcttattt ctttcatacg tgaacgagat
     1201 acgattatta ttcctagtct tgatcgatta gcaagaaata gtaaagatac aaaagactta
     1261 cttcaattgt ttcgtagtaa aggtgcttct attgaaatat tagacctacc gtcgtttaat
     1321 ggggtaactg accctgcatt aagagacttg ctaacaaatt tagtcataga agtgtttagc
     1381 tatgttgctg aaaacgaacg gaaaaaaatc aaagagcgtc aaaaacaagg cattgaaatt
     1441 gcaaagaaaa atggtcgtta tactggtaaa aagattcaat accatgccaa tgcaacagga
     1501 aaaaacaaat tgatttacga tgaaattgtt gatggtctga agaaaaaaga aactatttct
     1561 aaaatagcaa agaacgcagg agttacaaga aaaaccgttt atcgagtaaa aagagattta
     1621 ggaatgtaat atgttccagt tagaaag
//
LOCUS       DAJSNV010000210         1640 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSNV010000210 DAJSNV010000000
VERSION     DAJSNV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.216"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1368
                     /locus_tag="ON516_002876"
     CDS             <1..1368
                     /locus_tag="ON516_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3346290.1"
                     /translation="DLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYP
                     DGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTD
                     VTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDL
                     NKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTV
                     TDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKK
                     GTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPD
                     GSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGS
                     KGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNR
                     KKENE"
     gene            1502..>1640
                     /locus_tag="ON516_002877"
     CDS             1502..>1640
                     /locus_tag="ON516_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3346291.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TS"
BASE COUNT          645 a          248 c          349 g          398 t
ORIGIN      
        1 gatctaacag ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat
       61 gttactcccg gcggtacaat tgataccaac acaccaggta attacgaagg tgtcattgaa
      121 gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat
      181 cgttctgacg ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc
      241 acagtagatt taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta
      301 acagatgtta ctcccggcgg tacaattgat accaacacac caggcaatta tgaaggtgtc
      361 attgaagtaa cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca
      421 gacaatcgtt ctaatgcaga taaatacaat cctgaaggac aaaaagtgac tacagactta
      481 aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatttacc aaaaggaact
      541 acttatgctt ggaaagagaa agtagatgtt agcacagctg gaaataaaaa aggtactatt
      601 gtagtgacat atccagatgg atcatctgat gaagttgaag tagacgttac agtgacagat
      661 actcgttccg atgcagataa atataatcct gaaggacaaa aagtgactac agacttaaat
      721 aaagagccag atgcatctga gggaattaag aacaaagaag atctaccaaa aggaactact
      781 tatacttgga aagaaaaagt agatgttagt acagctggaa acaaaaaagg tactgtagta
      841 gtaacatatc cagatggatc taaagaagaa gtagaagtta ctatttctgt agtagataaa
      901 aaagcaccga ataaacctca agttgatcct attacagagg gtgaccaaat tgttactggt
      961 aaaactgaac caaatgcaga ggtgacagtt acattaccag acggaagtca acaccatggg
     1021 acagctgata aaaatggtaa ttttacagtt aaagttccta aattagaggc agggacaaaa
     1081 gtgatagtaa ctgcaactga tgaatctggt aatactagtg aacctactaa tgtagttgtt
     1141 tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa
     1201 ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa agtttttcct
     1261 aagacgggag agagtgacag taatatcttt actattagcg gtggtttaat actgttagga
     1321 acgttaggat tattagggta taaaaatcga aaaaaagaga atgaatagaa aatcaatttc
     1381 ttaagtcttc tctgagaaga ggaagaatct ttttcttcct ctttttgctt ttttatgatg
     1441 ggaaataagt gttagttaaa gatgaatata ctttttatag aaaggaaaag aatgtaatta
     1501 agtggaaaaa atagtaatta ttggttcaaa ccattcaggg ctagccgcag ctcgatttct
     1561 aaacttaagc gattcaaatt atgaagttat tctaatcgat aaaaaatcag aaattagtta
     1621 cttggaatgt gggacatcgt
//
LOCUS       DAJSNV010000211         1622 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSNV010000211 DAJSNV010000000
VERSION     DAJSNV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.217"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..400
                     /locus_tag="ON516_002878"
     CDS             2..400
                     /locus_tag="ON516_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3346292.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ON516_002879"
     CDS             384..1079
                     /locus_tag="ON516_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3346293.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          612 a          233 c          323 g          454 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tc
//
LOCUS       DAJSNV010000212         1608 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSNV010000212 DAJSNV010000000
VERSION     DAJSNV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.218"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..147
                     /locus_tag="ON516_002880"
     CDS             <1..147
                     /locus_tag="ON516_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346294.1"
                     /translation="GKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLF
                     DGGK"
     gene            206..469
                     /locus_tag="ON516_002881"
     CDS             206..469
                     /locus_tag="ON516_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HCR3346295.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            469..804
                     /locus_tag="ON516_002882"
     CDS             469..804
                     /locus_tag="ON516_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3346296.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            878..>1608
                     /locus_tag="ON516_002883"
     CDS             878..>1608
                     /locus_tag="ON516_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3346297.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGT"
BASE COUNT          546 a          229 c          348 g          485 t
ORIGIN      
        1 ggtaaatcaa tgacgaactc agaactagaa tccattcctc aacaaaatca aggagacgct
       61 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
      121 ttaaaattgt ttgatggtgg aaagtaactt cttgtatttt ggtgtataca agtatatact
      181 aatgtgtgag agaaggaggg attccatgtt agtaaaagaa atcgaaatga aaaaatgggg
      241 aaattcacaa ggtattcgtt ttggaaaaga agaattgtca gttctaggag aaactaatac
      301 tgaaaattta aaattcgaaa tggtggtaac agaaggaaaa attgttttaa ctcctaaaaa
      361 taaaaagcca gaaactttag atgaattgtt cgccaattat gaaggagaac ccttaggaaa
      421 agaagacaaa tatgaatggg gaaatatgca aggtagggag atcatttaat gaaaaagaac
      481 tatcatgatt tatcgcaagg agatcttgtt tatattgatt taaatcctac caaaggacat
      541 gaacaacggg gaaaacgacc atgtattatt ttatctaaac ctaataaata tttgaactat
      601 atgattggtg tcgcaccgat aacaacaaaa gcgaagcctt ttcctgtaca tgttgatttg
      661 aatggtacgc aaaaaataaa aggacaaatt cttttggaac accatcgaat gattgacatt
      721 gagagtagag gttttcagtt tgtggaagct gtgccaaagg aaattttaga agaatgtatt
      781 gaaaaaatta aattatttta ttaaaagcag cgaagaataa ttattatttc ttcgcttttt
      841 tgttgtttaa aattaaaaag ttaggagtac tgactatatg gaaaatgata caaatatagt
      901 tatggaacat ttgaagcgtc gtgcaaagcg acgcttattt ttgtggttgt ttggtacttc
      961 tgcaggtttg attactattt tgatcactgt gttcgtaacc ctttttctaa tacttgcggc
     1021 gggttcgatt gataattcgg attccgattc aagtagtggt ggcgaagctt ttacaggaga
     1081 gtattcggaa ggcttaccga tttataaaga aatcaaagga agaggccctt tttctgatga
     1141 gattgcgcaa tatgccgtag gtgcagcagt gaaatataaa ttattaccaa gtgtgattct
     1201 ttcgcaatac ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa
     1261 ctattttggg attacttggt ttgatggttg tctgtttcct aaaggaactg cacgaggaat
     1321 tggtggcatt gaagggggct ggtacatgaa gtttcccaac tcaaaagctg cattctccta
     1381 ttacggcttt atggtagcca cacaatcaaa ttttaatgca tgtgtaggaa ataaaagccc
     1441 tggcgcaagc ttattgatct taggacgagg tggttatgcc gctgcaggaa ttactgaaga
     1501 tagtccgtat tatacaggtt gtatgtcaat tatcacttct aataaattaa ccgaatatga
     1561 tgaattcgcc atcaaacatt ggggtgaagg tggcaataac aatggcac
//
LOCUS       DAJSNV010000213         1598 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSNV010000213 DAJSNV010000000
VERSION     DAJSNV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.219"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..457
                     /locus_tag="ON516_002884"
     CDS             35..457
                     /locus_tag="ON516_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346298.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            467..670
                     /locus_tag="ON516_002885"
     CDS             467..670
                     /locus_tag="ON516_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346299.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            881..1465
                     /locus_tag="ON516_002886"
     CDS             881..1465
                     /locus_tag="ON516_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346300.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          561 a          227 c          295 g          515 t
ORIGIN      
        1 cgttttctta tttttgattt cgaggtgata gacaatgaaa aataagttta ttttatcttt
       61 aggtatttta actattggaa ttagtgtaac tggaactaca attcctacat ttgctgatag
      121 tacaaatgaa ccaactactg tagtagataa taaccaagaa ttaactcctt tagataggaa
      181 cggaatagcc attggtaacc caactaacga cttaactgtt actgaaaatt atttaatagt
      241 ttctccatta cttgctcgag cagcttctaa aacgatacga gtgacaggaa cgatgatttt
      301 tgataaagtt ggttggcctt ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa
      361 tggttattgg tactatggaa aattgtattt acaaagttat aaatcagctg gaaacaattg
      421 gattgcgact tattcaggaa ccttaactag aaagtaggga ttttttatgc cagaatttgt
      481 gaacacacca aatttttatc aagatagcga tattctaagt aaaattaatt taccgaaaga
      541 agaatcagct ttacaagttc agcgaacttt acaggagaca caaaaaattc aaaaatcaat
      601 ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa
      661 agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa
      721 gtgttcataa aatcacccat ttctggacac tttctttttt tgctttattt ttcaaaaaaa
      781 caattcattt catgttcata aacccataac aatatctatg ttcagttaaa ctatatggat
      841 aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag gatacgcccg
      901 ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata aatttggttg
      961 tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag gattagaaat
     1021 ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag atcgtttagg
     1081 tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt
     1141 tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac agctcatgtt
     1201 tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac gatccgcagc
     1261 aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa aattgtctga
     1321 acaagatttg gaattactga agactttagt ggatagcggt acacctatta agactattgc
     1381 ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa
     1441 aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt tttgccattt
     1501 atctagaaaa gtgacaatag tatgagaatt ttttgtaagt gtctactgat aacttattct
     1561 caaatgttgt aaggatttta ttcatttgtt aatattaa
//
LOCUS       DAJSNV010000214         1573 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSNV010000214 DAJSNV010000000
VERSION     DAJSNV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.220"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            277..1446
                     /locus_tag="ON516_002887"
     CDS             277..1446
                     /locus_tag="ON516_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3346301.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          577 a          220 c          256 g          520 t
ORIGIN      
        1 atttgttggt agtataattt aaaggtaata agttattctt ttattactat gtttgggttt
       61 tatagcaaag aaagtttaat aagagtttgg acctagcaaa caatattaat gaggtcatta
      121 aatcttatta tctttagtga tataacgaaa tatctacaca gacaaaatga tagcgtaaaa
      181 tattttgtgt aaatagatat ttagggtaga aaaaaagaaa gtccattctg taaaattaaa
      241 gttaccacaa ccgaaatttt ataggaggac tttctcatgc ccaattttac tacagaaatt
      301 atggaaacac taatcaataa aggtgattta gatgaattat ttcgatgtca tttagaatta
      361 gctgttaatt cattattgca ggctgaatta acagcttttc ttgattatga aaaatatgat
      421 cgtgctgggt tcaattcagg taattcccgt aatgggaatt attcacgttc attcagaaca
      481 gagtatggag aattaaattt ggtaattcct agagatagaa atggtaagtt ttctcaacaa
      541 actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat tcagctattt
      601 caaaaaggaa ttactatgtc tgaaatctct gaattgattg aaaaaatgta cgggcatcac
      661 tatacgccac aaaccatttc aaatatgact aaaatagttt ctgaagatat tattgccttt
      721 aaagaaaggt ccttagaatc cagatactct gtcattttta tggatgctac tcacattcct
      781 ttaaaaagac aaactgtttc aaaagaagca gtttatattg taattggcat tcgattagat
      841 ggaaccaaag aggttcttgg gtttagtctt gctccaactg aatctgctta tgtttggaaa
      901 gagattcttc aagacctaaa agaccgtggt ttaaaagagg ttttattagt cgtaactgat
      961 ggtttaagtg gcattaacga tagtattcat agtgtttatc caaatgctca atttcaacaa
     1021 tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa
     1081 atctgtagcg atttcaaatt agtttatcaa gcaagttcaa aagaagaagc aaataatcaa
     1141 ataaggttta tgatagataa atggaaaaag cagtatccac gagtggttaa attgctcatg
     1201 aatcctgcta tattaacctt ttataatttc cctccgtcaa tcagaagaac tatttattca
     1261 actaatttga ttgaaggttt taacaaacaa ttaaagaaat ataccaaaag aaaagagcaa
     1321 tttcctaatg aagaatctct ggagagattt ctagtatctc aattcaacaa ctataatcaa
     1381 aagtttctat gccgagttca taaaggtttt aaagaaatac atgatacatt agaatcaatg
     1441 ttttaacttg atcaacagaa tgggcttttc catttacaca aaattattga cgctcccgac
     1501 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     1561 cttggtagca cgg
//
LOCUS       DAJSNV010000215         1565 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSNV010000215 DAJSNV010000000
VERSION     DAJSNV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.221"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..195
                     /locus_tag="ON516_002888"
     CDS             <1..195
                     /locus_tag="ON516_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346302.1"
                     /translation="EAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEG
                     SSDSTVQSNTSESSESNEVK"
     gene            195..>1565
                     /locus_tag="ON516_002889"
     CDS             195..>1565
                     /locus_tag="ON516_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3346303.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERV"
BASE COUNT          573 a          264 c          299 g          429 t
ORIGIN      
        1 gaagcccagg aacaagcaag aaaagagtat gaacaacaat tagaagaatt attgaatcgt
       61 accaatcagt caacaacaga gtcaagcatg gaaaatagca aagagagtga ttctagtaag
      121 gctaaagaag gatcaagcga ttcaactgta caatcaaaca cttccgaaag ttcagaatca
      181 aatgaggtga aataatgcgt gttagagaaa caacgaagcg aaaagacaaa cgattcaatt
      241 tagaaaagaa taggtatctt tctcatttct atcgctatac tccctcagga gaagagctac
      301 cagaaaaaca tgacaaacgc atttcgaaaa agatttttta tagtggactt gtatacctcc
      361 ctttagagta tttagtaaca agagtaccag aagttttcaa ccgattggaa attgttaagg
      421 gaagcttaca aggaataaat ctgtttcaat tacaggaaat gccacaggtc tttcattttg
      481 attcagctgg tttttggaat ccatttactc ctacaactag tttgggtaca cgtttagcaa
      541 tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac atgtggtttc
      601 gtcctctagc agatggacaa aaaggggata atcgattaat gaccttaaaa gaaatccaac
      661 agatttatcc gcaaatacct gaaacaaaaa ctacttttcc aggttatgga ggtattccaa
      721 tcacacatta caaaaaatat tggtacattc aaacagatac tgttaatacg tgtattgttg
      781 gtacctctcg atcagggaaa ggtcagattg aagtgttagc caccattgac aatttatctc
      841 gtgcagaaaa acaaagttca atggtcgtta atgatccaaa aacagaatta tatgttggta
      901 gtaaagatga actagaagct agagggtatg atgtatatgc ctttaatatc accgatccac
      961 tgcaatcaat gagtgataat ccattggcac tcatcgtgaa atactggaaa cgaggagaca
     1021 tagatactgc aacacagtta accaatactt tcacaaatac gatttattat gatgccaacg
     1081 cttcagataa caaatacttt aacgaaaacg cgcagaaagc tgtcaatgcg cttatttttg
     1141 cgttgttaga acaagctgat caaacaggag attactccaa attgacaccg aataatctcg
     1201 ttgagcttct ttctgagatt ggcggtttta attattatcc agatccagac aatgaattca
     1261 aacagttgaa tgccttagat gaatttatga atcagttgcc acctggaaac attgcaaaaa
     1321 aacaatatgg ggcaacgaaa attggatctg ataaagcgaa gggaaatatt ttatcaacgg
     1381 caatcacggg attgaatcca tttactctaa cgaaaattgc aaagatgact tctcaatcaa
     1441 cgattgatta taaatcggtt gggtttccaa aatacttaga tttgaaatta catgaaagtc
     1501 ttttaaatca acgagtcgta gtagaatttt atcataaggg aaagaaattt catgaagaac
     1561 gtgtc
//
LOCUS       DAJSNV010000216         1527 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSNV010000216 DAJSNV010000000
VERSION     DAJSNV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.222"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(75..159)
                     /locus_tag="ON516_002890"
     tRNA            complement(75..159)
                     /locus_tag="ON516_002890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Leu,seq:caa)
     gene            complement(177..248)
                     /locus_tag="ON516_002891"
     tRNA            complement(177..248)
                     /locus_tag="ON516_002891"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Gln,seq:ttg)
     gene            complement(258..330)
                     /locus_tag="ON516_002892"
     tRNA            complement(258..330)
                     /locus_tag="ON516_002892"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:His,seq:gtg)
     gene            complement(392..476)
                     /locus_tag="ON516_002893"
     tRNA            complement(392..476)
                     /locus_tag="ON516_002893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:caa)
     gene            complement(497..567)
                     /locus_tag="ON516_002894"
     tRNA            complement(497..567)
                     /locus_tag="ON516_002894"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(533..535),aa:Cys,seq:gca)
     gene            complement(613..684)
                     /locus_tag="ON516_002895"
     tRNA            complement(613..684)
                     /locus_tag="ON516_002895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Gln,seq:ttg)
     gene            complement(696..768)
                     /locus_tag="ON516_002896"
     tRNA            complement(696..768)
                     /locus_tag="ON516_002896"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(733..735),aa:His,seq:gtg)
     gene            complement(771..844)
                     /locus_tag="ON516_002897"
     tRNA            complement(771..844)
                     /locus_tag="ON516_002897"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(810..812),aa:Trp,seq:cca)
     gene            complement(851..931)
                     /locus_tag="ON516_002898"
     tRNA            complement(851..931)
                     /locus_tag="ON516_002898"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(895..897),aa:Tyr,seq:gta)
     gene            complement(933..1008)
                     /locus_tag="ON516_002899"
     tRNA            complement(933..1008)
                     /locus_tag="ON516_002899"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(973..975),aa:Phe,seq:gaa)
     gene            complement(1044..1116)
                     /locus_tag="ON516_002900"
     tRNA            complement(1044..1116)
                     /locus_tag="ON516_002900"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Asp,seq:gtc)
     gene            complement(1126..1198)
                     /locus_tag="ON516_002901"
     tRNA            complement(1126..1198)
                     /locus_tag="ON516_002901"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1163..1165),aa:Val,seq:tac)
     gene            complement(1214..1285)
                     /locus_tag="ON516_002902"
     tRNA            complement(1214..1285)
                     /locus_tag="ON516_002902"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1250..1252),aa:Glu,seq:ttc)
     gene            complement(1300..1389)
                     /locus_tag="ON516_002903"
     tRNA            complement(1300..1389)
                     /locus_tag="ON516_002903"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1353..1355),aa:Ser,seq:gga)
     gene            complement(1429..1502)
                     /locus_tag="ON516_002904"
     tRNA            complement(1429..1502)
                     /locus_tag="ON516_002904"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1466..1468),aa:Asn,seq:gtt)
BASE COUNT          441 a          396 c          348 g          342 t
ORIGIN      
        1 gatttggagg taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc
       61 tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac ggacactgga
      121 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg
      181 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
      241 ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
      301 ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttgttt aatttctcaa
      361 taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc
      421 cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt
      481 tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc
      541 tctgccttac cacttggcta taccgccatt atactattat caatctataa aataaagaat
      601 aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt
      661 gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac
      721 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc
      781 agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc
      841 ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt
      901 ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc
      961 gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat
     1021 tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt
     1081 gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga
     1141 tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa
     1201 cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt
     1261 gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac
     1321 ccttgagaca gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga
     1381 caactctcca gagtgaaaga agctcaatac tctttctcaa aatgagaact ccggcagtag
     1441 gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatgccg
     1501 gaataacatc agcgtggcga cgtccta
//
LOCUS       DAJSNV010000217         1526 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSNV010000217 DAJSNV010000000
VERSION     DAJSNV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.223"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            15..1481
                     /locus_tag="ON516_002905"
     CDS             15..1481
                     /locus_tag="ON516_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3346304.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          495 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctg
//
LOCUS       DAJSNV010000218         1453 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSNV010000218 DAJSNV010000000
VERSION     DAJSNV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.224"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1453
                     /locus_tag="ON516_002906"
     CDS             <1..>1453
                     /locus_tag="ON516_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346305.1"
                     /translation="PTDKRNALTAKNFKTANINGIITPEQAKGMAGNDKSGLSDIQKD
                     RLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRYSSKPGIILAGESSLAVAYL
                     FILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILELRIY
                     TLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNSLLS
                     MFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGHRKDVTPDNGEEQSDDKATT
                     VPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAEDLTEKAKDTLEDKLDGTRA
                     KELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGIVIP
                     SVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTDPEQYRYKPNSDLENEKRIS
                     DLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSE"
BASE COUNT          547 a          229 c          307 g          370 t
ORIGIN      
        1 taccaacaga taaaagaaat gctttaacag cgaaaaattt taaaacagcg aatatcaatg
       61 ggattattac tcctgaacaa gctaaaggta tggcaggtaa tgataaaagt gggttatcag
      121 atatacaaaa ggatcggtta aaggcattac aataccgatt ggaactagat gataatggga
      181 aagaaattcc tgttcaaatt agtgacgaag gcttagcaaa atatgtctat acttcagggt
      241 atcgtcgata cagtagtaaa ccaggtatta ttttagctgg tgaatcttct ttggcagtag
      301 cctatttatt tatcctattt acgatcgtta cttgcattat tgagctggta tttaaaaaat
      361 tctatttagt gattgccgcg tcaacggact ttgagacagg tcaacggatg aaaagtgcga
      421 tagaagatat ttctcaatcc ttcttgttat tggcgtttac gattttagaa ttacgaatct
      481 ataccttaat gctttcaggt atcggtgatt tgcatgcggc aggaaagatc aatggattct
      541 tgtatgtcgt tgctttgatt gtactaacca ttgcgctatt caaaggctct caatcagtta
      601 cgaaaatttt cggcgttgat acaagcctga aaaacggcag taattctctt cttagcatgt
      661 ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg
      721 gtaagagtgg tttaagccat ttaaatgata ttcgtaaaaa tggatttggc catcgaaaag
      781 atgttacccc tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
      841 gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
      901 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa
      961 aagccaaaga caccttagaa gataaattag acggcacgag agctaaagaa ttggctgaag
     1021 ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gaagtcaaag
     1081 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtgaaa gctaatacaa
     1141 caaccgaaaa agaaaatcaa gaaggtatag tgattccttc agtcaaagat caccaagaag
     1201 cgttgcactc taatgaaaat tcgattgaag agaaaaattc catagaagaa agaaattcag
     1261 aagaaggaaa aaatggacag ttaccaaaaa ctgatccaga acaatatcgc tataaaccta
     1321 acagtgacct tgaaaatgag aagcgaattt cagatttatc aaaagtaaat caagaagaca
     1381 aagcagaaaa tgagggacag ttaccaaaaa ctgatccaga acaatatcgg tatagatctg
     1441 ttaactccga aaa
//
LOCUS       DAJSNV010000219         1450 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSNV010000219 DAJSNV010000000
VERSION     DAJSNV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.225"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..132
                     /locus_tag="ON516_002907"
     CDS             <1..132
                     /locus_tag="ON516_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3346306.1"
                     /translation="ECDCGERAKCIKSYRLYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            132..383
                     /locus_tag="ON516_002908"
     CDS             132..383
                     /locus_tag="ON516_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346307.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLSPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            399..836
                     /locus_tag="ON516_002909"
     CDS             399..836
                     /locus_tag="ON516_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346308.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNVKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            1281..>1450
                     /locus_tag="ON516_002910"
     CDS             1281..>1450
                     /locus_tag="ON516_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346309.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPAL"
BASE COUNT          542 a          221 c          304 g          383 t
ORIGIN      
        1 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca
       61 acatgcggga aaaaatatac cttgtcttat ggcgattaca ttttgattga tgagaaagga
      121 aagaaacgct gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt
      181 tggtggacaa agaagtcatt tctgaaaaag tagcggaagc tttacttagt ccgcggtact
      241 ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga
      301 agattgcagg atatgaactg cattatcaag aaaacatgga aacttatgaa aacgaatatt
      361 ggttaaccaa attagagaac taaaaaggag aaaaaaatat ggctcaaaat tactatgacg
      421 aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg acgttcctat
      481 acaacgaaac acgtgttcca aagaaacatt ataaagaaaa attgtcggta gctgtggaag
      541 aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtactatcga acactggaag
      601 aactgaaaaa gcaaaatgta aaatggtttt tccaagcctt actatgcttg gaagtaggcg
      661 tcaaaccaag cacaatcaaa ccttctgaat accaagcttt agaactaacg tatgcgaaat
      721 ttatcgaaac gaaaaaagcc aagactgttt caagtgaatg gttagattat tttgaaaata
      781 tcaataagta tggcgcatat tacaccatga aaaaggaaga caacgaaaat gaataaggtg
      841 atttatcaac taacaacgga ttccatatca acgatttgaa agaaaaaaag gtcaataatc
      901 atttataccg aaaagtgaga atgtgtgaaa taaggacaaa aaaagcctag taactaaggg
      961 aatggttctt atttcgcacc aatcgctcca accgcccccg ccgcttaggt gatgcgaaat
     1021 aaacgggcaa agcccgataa cttaagagaa agaaggtgat ccatttgaac aagattattc
     1081 ccttattgtt agtactgttt gttggcgaaa taattatgcg acatagtgca gctgtgcatt
     1141 taaacttcgt agtatttttg gtaatactgg tgttttgttt gatcttttgg cgctttgtgt
     1201 tagcccttgt gagtctaata ttcaagttga ttgcagccat atttttcata gggttatttc
     1261 gatgaaagga gagagtacta atgccaattg caataggaaa taaacggtta cccgttacgt
     1321 tggatgaaaa gagacaaaaa gaattgcaga aactaaagca gaagtacgga aaaagtgaat
     1381 ctaagattat gtgtattgcg ttagatctat tgatcgcgca agaaaaagca ggatttgatg
     1441 taccagcact
//
LOCUS       DAJSNV010000220         1441 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSNV010000220 DAJSNV010000000
VERSION     DAJSNV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.226"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..120)
                     /locus_tag="ON516_002911"
     CDS             complement(<1..120)
                     /locus_tag="ON516_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346310.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSS"
     gene            395..937
                     /locus_tag="ON516_002912"
     CDS             395..937
                     /locus_tag="ON516_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346311.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1093..>1441
                     /locus_tag="ON516_002913"
     CDS             1093..>1441
                     /locus_tag="ON516_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR3346312.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          563 a          177 c          277 g          424 t
ORIGIN      
        1 agaagaaggc gtaagctcat actctcgaat aatttctgcg cgaggcttac cagcgagata
       61 gagatcgacg atttgttgtt tgaattcttt tgaataggtt cttcgttgac gtcttgacat
      121 gaaaaatcct ccagtgtgtt ttttattatt ctacacacct tattttttct gtctagtcta
      181 gtgtagccga ttcaaattga tgaagcagat cccaaagggc tgcttcatca attttttcgt
      241 aagtgtccgt acacttactg tgcttgctca tatattaatc gatcatatac taatcgtgta
      301 agaacaactt taagcataat acttatattg ttataccata gtttggaaaa ataaaaaatt
      361 atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag ctataaaatg
      421 ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa gaggagcatg
      481 cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa gtaatgaaaa
      541 caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt caattaatgc
      601 aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa agaaagaata
      661 tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa attatatagc
      721 tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac taaagtatgc
      781 tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag aaaagatggg
      841 cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg gtatatttga
      901 aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca tactaaaata
      961 ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt
     1021 aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct
     1081 aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa
     1141 gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca
     1201 agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca
     1261 gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt
     1321 gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca
     1381 attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca
     1441 g
//
LOCUS       DAJSNV010000221         1433 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSNV010000221 DAJSNV010000000
VERSION     DAJSNV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.227"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(405..1019)
                     /locus_tag="ON516_002914"
     CDS             complement(405..1019)
                     /locus_tag="ON516_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3346313.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          465 a          276 c          239 g          453 t
ORIGIN      
        1 ctgttggaca attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact
       61 ataattactt tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa
      121 gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca
      181 ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact
      241 ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa
      301 gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat
      361 gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg
      421 tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca
      481 agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt
      541 aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta
      601 tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca
      661 ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga
      721 agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct
      781 tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc
      841 ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc
      901 aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc
      961 tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt
     1021 tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt
     1081 acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa
     1141 aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca
     1201 tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc
     1261 gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat
     1321 tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat
     1381 ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaa
//
LOCUS       DAJSNV010000222         1414 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSNV010000222 DAJSNV010000000
VERSION     DAJSNV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.228"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..424
                     /locus_tag="ON516_002915"
     CDS             17..424
                     /locus_tag="ON516_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346314.1"
                     /translation="MFFLPLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
     gene            411..863
                     /locus_tag="ON516_002916"
     CDS             411..863
                     /locus_tag="ON516_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346315.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            856..1254
                     /locus_tag="ON516_002917"
     CDS             856..1254
                     /locus_tag="ON516_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346316.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          550 a          172 c          243 g          449 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc attaaaatat cttgaaattt actctgttaa
       61 aaaaaataaa ttaattggtg aaaatgattc tccaataaaa acacctattg agctaagctt
      121 aagaaatcgg atgggaatac tccaaagatc ggaaatagta aaagaacttt tttatcataa
      181 atttagttat gaaataagac tagatgatac ttatgagcat tatagaattg tttttttcgt
      241 atacaattct atagaagatg ctactgcgac tactgcatta acattcggtt ttacgaaaaa
      301 tggaacaatc aattcggata aaacccgtca agctgctaca gaaagtgatg atatatgtaa
      361 gaaagtatgc aatggggaag agaattgctg gataggggag gaaaagttga atgaaatcta
      421 ttaatgattt attaaaaaaa catcctgatt tatataaaaa attaaattct gaacaaagaa
      481 acctgtccaa acaattactt aaaataagat taaaaaaaca aaaaaacgtg acagaattct
      541 caaaaatgtt aggaataagt gaaaaaaaat atcttgaatt tgagttcgga gatatgcata
      601 ttcctgttgc agagtacaat aaacttttga aacaagttga aaatttgtcc tataaagatt
      661 caatgaattt cactattaag gttgctggcg gccctattaa atatgaccgt attgaaaata
      721 aaccttatat tcggaataaa agttactcag tatttaaaag tagaaagaga aaagaagtat
      781 cgtcaaatat ggtctatgct agtgttaatt accaagatac gagccgctat attgtagaaa
      841 aagaggtata tgtacatgga taaaggaaat agtgttactt ttgcacttga aaatattagt
      901 ttgaataaaa ttcattttga attaactgat aattttagtt atggagattt agagtttaat
      961 attgaacttg gatataaaga agacgaagca gagtatctag aaaaaaaact tgtttcttta
     1021 cgagctcaca ttgtaggggg gatggaagga gcaccggtag atattttact cttttcagtt
     1081 attcgtgttt ctacaaatga acagccatat aaaaaattag tagaagaatc tatgggagag
     1141 ctagctaaac ctatcctgaa taaagcttct aatttgattt ctaatttata tagcgaagta
     1201 ggagttatgc ctacagttat tgatttatat cagctatata gtcaacaaaa ataaaataca
     1261 agtaatagag ttatctattt aaatttgcaa gacaaagacc tcaaccagtc atccttggtt
     1321 gaggcttttt tgctctgtaa ttaactcgta tagatcctgc aagatagttt gtttctgaat
     1381 aaatgattga tatgaagtat ttagatgact gtat
//
LOCUS       DAJSNV010000223         1390 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSNV010000223 DAJSNV010000000
VERSION     DAJSNV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.229"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..638
                     /locus_tag="ON516_002918"
     CDS             <1..638
                     /locus_tag="ON516_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCR3346317.1"
                     /translation="EKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMS
                     ISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLY
                     GVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSYFACS
                     YVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            816..1037
                     /locus_tag="ON516_002919"
     CDS             816..1037
                     /locus_tag="ON516_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3346318.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          476 a          133 c          285 g          496 t
ORIGIN      
        1 aagaaaaaaa ttatatagat ttttgtgata ttacaacaat tataggttgg tttctggcaa
       61 ttagttgtgc atgctttatt acattatttc agccgttcat gattatctgg gtgggaagtg
      121 ataatcttat gagtatttca tttgttatat ttatgatagt tcattttttc ttatatgaaa
      181 ttgatcagtt aattgggtta tacaaagatg ctgctggaat atggtacgca gacaggttta
      241 gaccattaat tacagcaatg ataaacattg tattaaatat catcctcgtt aattggttcg
      301 gattatatgg agttttggcg gcaaccattg tttcagtatt gttggttgat ttaccctggt
      361 tattatataa tgttttccaa aatatattta aatttgaatc ggcaggtaaa tatgtttgga
      421 agctcataag tttgtgtgta atagcttttg ttgcttctta tttaagttat tttgcttgtt
      481 cgtatgttaa tatcggaaat ttcaaagtga taagtttagt gataaattta gttatatcgg
      541 ttgtcatatc gattttagtt attcttctgt ttaatatccg taataagaag tttaagtata
      601 ttttgaaagt tcttacgtcg gtattggtaa aaagatgaga agagacatca gaatgatata
      661 taaagtaact acaattttta ggctgccgaa taggcgaacg atgtatagtt tttgttccaa
      721 cgaagactct tgttgaaatt ggagatgata tgaaggagtt acaattttga ctcatgttta
      781 tgattgatcg gttttaaaag tagtatggag aagctttggg atcatcggag aggatcaaaa
      841 taggtaataa tgtttttata ggaatgaagt ctagaatact aaagggtgta catataggaa
      901 ataatgtagt tataggtgca aatagtttag taaacaagga cgtatcagac gattgtgttg
      961 ttgcaggaaa tctagcaaaa gtaattatgt ccctagatga atattataaa aagataaaca
     1021 atatgaagaa tccgtaaaac ttgtgaaact ataccgagaa taatatggga aaaatattga
     1081 tgaaaaagca ttatatgagt ttttagggct gtttacagat ggagatgaca atttgtcgaa
     1141 gagttgggag caatgcaaaa tttagtaggg gattataaac agactgacaa ggtatttaaa
     1201 gaacatgcac aagtatttca taatattgaa gaatttttga atactgtgag ataggagttg
     1261 ctacattgag aaaagtatgt atttttccaa tgtaaaagat tacgagtttg aaaacgacac
     1321 cataatatta ttttttaaga agatattata gagcttagga acggaagttt atttaaggat
     1381 gttgaataaa
//
LOCUS       DAJSNV010000224         1368 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSNV010000224 DAJSNV010000000
VERSION     DAJSNV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.230"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>1368
                     /locus_tag="ON516_002920"
     CDS             82..>1368
                     /locus_tag="ON516_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3346319.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAJSNV010000225         1316 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSNV010000225 DAJSNV010000000
VERSION     DAJSNV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.231"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ON516_002921"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ON516_002921"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3346320.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          451 a          175 c          221 g          469 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaa
//
LOCUS       DAJSNV010000226         1294 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSNV010000226 DAJSNV010000000
VERSION     DAJSNV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.232"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1244
                     /locus_tag="ON516_002922"
     CDS             <1..1244
                     /locus_tag="ON516_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3346321.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAJSNV010000227         1288 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSNV010000227 DAJSNV010000000
VERSION     DAJSNV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.233"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..250
                     /locus_tag="ON516_002923"
     CDS             <1..250
                     /locus_tag="ON516_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3346322.1"
                     /translation="YTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDY
                     LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            255..1202
                     /locus_tag="ON516_002924"
     CDS             255..1202
                     /locus_tag="ON516_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3346323.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          423 a          187 c          267 g          411 t
ORIGIN      
        1 atacacatct aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt
       61 taaacctgaa tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa
      121 aatgagagat tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt
      181 tttgtttcct aaatttttag aagattcttc ggagcttcaa gatatctata attatttata
      241 tcggaggtaa aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta
      301 ccttagccaa tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg
      361 gaaaagtaga aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg
      421 agaaatcctt gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag
      481 cagcagttgc tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt
      541 ttgaaagtgt attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga
      601 tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag
      661 agtccgtgat tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg
      721 tgctagatta ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct
      781 atggtccaaa tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc
      841 gctataagaa acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc
      901 agtctagaga ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg
      961 aggaagctgc actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg
     1021 aattgattca tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag
     1081 agcgttcggg tgatatccat gactctctag cagatattac taagatccag tcaattggct
     1141 atcaaccgaa atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt
     1201 aaaacatact aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg
     1261 agttttgtaa aatcaaatct gggataga
//
LOCUS       DAJSNV010000228         1268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSNV010000228 DAJSNV010000000
VERSION     DAJSNV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.234"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(106..477)
                     /locus_tag="ON516_002925"
     CDS             complement(106..477)
                     /locus_tag="ON516_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346324.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(479..880)
                     /locus_tag="ON516_002926"
     CDS             complement(479..880)
                     /locus_tag="ON516_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346325.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(894..>1268)
                     /locus_tag="ON516_002927"
     CDS             complement(894..>1268)
                     /locus_tag="ON516_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346326.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          405 a          228 c          166 g          469 t
ORIGIN      
        1 acgtagcgat catcggcgtt atgactatat aaagtatact ttcgttgatc cagaaagata
       61 caaagattaa ataaaaaaat aacaaacaaa ctcgcttgaa attcattagg tgagtttgtt
      121 tattattttt tctgcttctc gattcatcag cgccatatct gttttcgtaa tggtttcatt
      181 aggaatatag tcagcacatt ttcttttctc taacattcta tacccactat tatgtattcc
      241 taaggtattc aattccttaa ctgttgaaaa caaattccaa acggtttggt ggcttccttt
      301 tccgctactt ccattataaa taatatcata attttctttc aaatactcat cagatttttt
      361 aaaagcactg tagtatgctc ttgaaacagc agttcttcta taagattctt tagaattata
      421 tttatcatct gattcaattt gtttggaaat agataaatat tcattaaagt caaacatttt
      481 attcccccaa agttgctact acagatttcc ccatatattt atagtcagat aatacattat
      541 cgagagtatc caaaacatcg taatctgagg aaaacaattc agatgttgtt ttgataacaa
      601 agaaatacaa gtttaaatct tcatcatatt ttatattgat ttcagctttt ctttttaaaa
      661 ccgtatttat cacatcaaaa attttgccat cattacttga aattgtgtta atctcatttt
      721 ttgtagtaat tacaagttga ttatcatttt tagttttcgg agtagaactt gcatccacaa
      781 caactggaga tgctagaatt accgatgcgc aagcaaaaat cgcagcaaaa tttattaagc
      841 taccactccg atgtgtattt gatggtacta tatcaagcat ccgtatcgtc tccttaattt
      901 ttttccactg gtttgttgac cttgatatca aagatttgcc ttccaatctc agttccatta
      961 aatttaacaa cagcataatg ctctcctgct tcttcaacca agacatttct ggcgtttata
     1021 ttaaagttta tatcgttaac ttgaggaggc aagcttaccg atacttcacc cgtagaaaaa
     1081 atttgttttt cattcttgtt taaaacttca atttctacta tttttatctt ttctgtatcc
     1141 tctatctcag acaccacagc tgttacagaa aatgataaag cagtaggaat taaaggcgca
     1201 ttaatagaaa gagctggatt aattacttta ttaataacat tagtgccaaa ttgctgactt
     1261 tctacatc
//
LOCUS       DAJSNV010000229         1266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSNV010000229 DAJSNV010000000
VERSION     DAJSNV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.236"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1266
                     /locus_tag="ON516_002928"
     CDS             <1..>1266
                     /locus_tag="ON516_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346327.1"
                     /translation="ITPDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQIS
                     DEGLAKYVYTSGYRRYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIA
                     ASTDFETGQRMKSAIEDISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVV
                     ALIVLTIALFKGSQSVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGK
                     SGLSNLNDIRKNGFGHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAA
                     EGLGYVNERGLKGTAEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASA
                     VKEGLKDAYGEGQVNAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIE
                     ERNSEEGKNGQLPKTDPEQYRYKSNINSE"
BASE COUNT          461 a          191 c          273 g          341 t
ORIGIN      
        1 tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta cagtacaaaa
       61 agatcggtta aaggcattac agtatcgact agagttagac gataatggga aagaaatccc
      121 tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acgtcaggct atcgtcgata
      181 cattagtagt ccagggatta ttttagctgg ggaatcttct ttgtcggtag cctatttatt
      241 tattttgttt acaatcgtta cttgcattat tgagctagta tttaaaaaat tctatttagt
      301 aattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcga tagaagatat
      361 ttctcaatcc ttcttgttat tggcgtttac gattttagaa ttacgaatct atacattaat
      421 gctttcaggt atcggtgatt tgcatgcggt aggaaagatc aatggattct tgtatgttgt
      481 tgctttaatt gtgctaacta ttgcgctatt caaaggctct caatcggtta cgaagatttt
      541 cggtgttgat acgagtctga aaaacggcag caattctctt cttagcatgt ttgccttagg
      601 aaatattgcg aaaaatgtgg gtagtggggc taaaaacctt gcaagtgctg gtaagagtgg
      661 tttaagcaat ttaaatgata ttcgtaaaaa tggatttggc catcgaaaag atgttacccc
      721 tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata gtaagaaaac
      781 agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag aaggtcttgg
      841 ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa aagccaaaga
      901 caccttagaa gagaaattag acggcacgag agctaaagaa ttggctgaag ctgtgaagaa
      961 tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gcagtcaaag aaggactaaa
     1021 agatgcgtat ggtgaaggac aagttaatgc agcagtaaaa gctaatacaa caaccgaaaa
     1081 agaaaatcaa gaaggtatag tgattccttc agtcaaagat caccaagaag cgttgcactc
     1141 taatgaaaat tcgattgaag agaaaaattc catagaagaa agaaattcag aagaaggaaa
     1201 aaatggacag ttaccaaaaa ccgatccaga acaatatcgt tataaatcta acattaattc
     1261 tgaaaa
//
LOCUS       DAJSNV010000230         1237 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSNV010000230 DAJSNV010000000
VERSION     DAJSNV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.238"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..702
                     /locus_tag="ON516_002929"
     CDS             <1..702
                     /locus_tag="ON516_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3346328.1"
                     /translation="FFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            692..>1237
                     /locus_tag="ON516_002930"
     CDS             692..>1237
                     /locus_tag="ON516_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3346329.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLK"
BASE COUNT          470 a          152 c          230 g          385 t
ORIGIN      
        1 tttttcaaag atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata
       61 aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa tattgttact
      121 ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct
      181 attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc
      241 aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat
      301 actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat
      361 tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt
      421 ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg
      481 tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat
      541 atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct
      601 gattcaatgg ttcggaagat taccacaaaa attaaaacag aattgacaag acaaatgaaa
      661 cgagatgctg gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat
      721 taatcaacat tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc
      781 agctagtcga gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa
      841 aggctattac agaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga
      901 tgtggtgcag gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga
      961 ttttattctt tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga
     1021 atttaaagga acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagcattcg
     1081 accatttaag gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat
     1141 ttataaccca gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc
     1201 ttctttttat attaataagg acaaatatga tttgaaa
//
LOCUS       DAJSNV010000231         1232 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSNV010000231 DAJSNV010000000
VERSION     DAJSNV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.239"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1215)
                     /locus_tag="ON516_002931"
     CDS             complement(<1..1215)
                     /locus_tag="ON516_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346330.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIY"
BASE COUNT          500 a          180 c          198 g          354 t
ORIGIN      
        1 atagattgac tctttgtttg cataatcagt atgattgtaa ttttcagcta tagcaacagc
       61 gtcttcaggt gctataagtg ttaatgaatc tttgttagca tggatagaat tgcttactcc
      121 tgagagatgc aatattagtg caagaaacgt aaagaccatt attttccatg aattattttt
      181 agtagaaaaa atataataga tcactatact catcgtatag gctataaata aagaagaaaa
      241 accatttaac cgccagacaa attgtagact cttaactggt gtatggttaa aaagttgcca
      301 aggaaataaa ttggttgaac aaaaaagaac aaatacacca agaaagaaaa taaataaatc
      361 atcagcagtt aattttttta taaaaataaa agcaccaatc aatccgagaa ataagaataa
      421 tccaatagta tagttgttat agttattatt taaaatatgt gtcagcatat cactaggagc
      481 cataccactt aattcttttc ccagtgggac tttcaattct tgtgctctag attgttgaat
      541 aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg acggtagctt ttaaaagact
      601 taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat
      661 aaatacagaa accatagcga cagaaagtaa atgagtatac actactagcg tcatgccaat
      721 agacaaccag taccattttt ggtaatctct aatataaatt tcgtaacacc ccattagtat
      781 caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa gcacgatgga atatgtcaat
      841 ggcacgatag gcggaaaatg tataaataat cgaaaataac agcgaaatat aacgattgtt
      901 ttttatttgt ttcatagaaa aataagaaac gaccattgtt aaaaaagtga taaaaaaata
      961 gtagatatta taacttaaaa caagatttcc catcatttta taaaataaaa aagctggata
     1021 aagagtaagc catggataaa aaatattcat cattgttcca tgatgatcga agttattgaa
     1081 gtttactgga gaactccata cattatctag tcctatgatt cgagataaat gaaagtaagt
     1141 atcctgattg acactatgat agattccctt agacatgaat agagggatga cgtaagcaaa
     1201 acttagaaat aacataaaaa tgatatctat ta
//
LOCUS       DAJSNV010000232         1212 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSNV010000232 DAJSNV010000000
VERSION     DAJSNV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.240"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1212
                     /locus_tag="ON516_002932"
     rRNA            <1..>1212
                     /locus_tag="ON516_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          317 a          272 c          376 g          247 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacagagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gc
//
LOCUS       DAJSNV010000233         1190 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSNV010000233 DAJSNV010000000
VERSION     DAJSNV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.241"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            109..1149
                     /locus_tag="ON516_002933"
     CDS             109..1149
                     /locus_tag="ON516_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3346331.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          482 a          175 c          200 g          333 t
ORIGIN      
        1 attaaaaaac agttttaatg cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga
       61 gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt
      121 aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt
      181 attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta
      241 aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat
      301 tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata
      361 gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac
      421 aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa
      481 aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca
      541 gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct
      601 caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc
      661 ataccagaac ttaataataa tatattagac actaatagac acaatataga cactgaaaaa
      721 gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc
      781 attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac
      841 agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa
      901 agtgatattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa
      961 ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc
     1021 cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa
     1081 gtatcaagtg aaaaacgttt gaattatgca agctccccaa gcactattac gaaagactgg
     1141 ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat
//
LOCUS       DAJSNV010000234         1180 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSNV010000234 DAJSNV010000000
VERSION     DAJSNV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.242"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1180
                     /locus_tag="ON516_002934"
     CDS             <1..>1180
                     /locus_tag="ON516_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3346332.1"
                     /translation="LTKEKYKGQICLIYKGTLLYKPEECSHCLCVVPSRIIRWGTTTV
                     RLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKEN
                     TSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIM
                     MDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     K"
BASE COUNT          360 a          237 c          235 g          348 t
ORIGIN      
        1 tttaactaaa gaaaaataca aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta
       61 caagccagaa gagtgttccc attgtctttg tgtcgtcccc tctaggatca ttcgttgggg
      121 gacgacaacc gttcggttgt tactgaatga tgtgtctgag tatcgcactt atctggagct
      181 gaagaaacaa cgatttaaat gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt
      241 tgctgaaaaa cactgtttca ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa
      301 agagaatact tccatgacgg aaatcgctcg tcagaaaaat atttccactt cttcggtcta
      361 tcgtgtcatg aagcgattct accgcccatt gaatcctttc aaacagactt tgccaaaggt
      421 cttatgcttt gatgaattca aatcggttcg aagtgttgcc agtgcaatga gttttatcat
      481 gatggacgga caaagccatg cattattgga tattgtcgaa aatcgacgct tgccttattt
      541 agaacgctat ttttctcggt tttcattagc gactcgtgaa gccgttcaat tgattgttat
      601 tgatatgtat gcgccttatg tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat
      661 catcgatcgt ttccacatcg tccaacatat tggcagaact ttcttgaatc atcgagtgaa
      721 agaaaccacg gtacgactta aaggaaattc ccactctcaa cgagggttag ggaaaaaatt
      781 gaagcgctat tggaaactcc tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt
      841 ttggcgtgcc agttttaaag attatttgac tgaaagtgag attgccgatc gactgttagc
      901 tgcttgtccc aacttacgcc aaggctatca gttataccaa gacattcttt atgcggtaaa
      961 gaaaagagat cagtcactat ttgaggattg tttaactaga gacattagcg gtctgccaga
     1021 aacctataca accactttac gaacgtttaa aaagtttctg ccacagatcc aaaatgcgct
     1081 tcactacagc tattcaaacg gaccattgga atgcttaaac aaccacatca aagtattaaa
     1141 acgcaatgcg tacggctttc gcaactttta caatttcaaa
//
LOCUS       DAJSNV010000235         1171 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSNV010000235 DAJSNV010000000
VERSION     DAJSNV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.243"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(200..1015)
                     /gene="thiD"
                     /locus_tag="ON516_002935"
     CDS             complement(200..1015)
                     /gene="thiD"
                     /locus_tag="ON516_002935"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3346333.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          310 a          256 c          193 g          412 t
ORIGIN      
        1 caaacatgag aagctgtttt ttgaacttgt tccttgtgac tcggagctgg acggcaggct
       61 cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
      121 gttaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcaact
      181 ttttcacaac ttctttcttt tactctaatt caccagctgc ccaatgattg atggggccat
      241 atttgtgccc tacatttatt tcttcacgaa ttgcacggtt tacatagtct tttgccaatc
      301 ggatcgcttg ttcaactgtt tttcctttcg ctaactcagc tgcaatacat gcagatagcg
      361 aatcgccagt tccgttgatc cggtcggtag gataaaatgg tgcatttaac caaaatgatt
      421 ttcctgattc gaacaatacg tagtcattga ctgtttcttg ctgcgcattt ttagcatgat
      481 gtcctttgat cataacgttt tttgcaccca tatcttgcag ttcttttgct gcttgcacaa
      541 aatcttcttc agctgctaat gtccgtccag tcagcttttc tgcttcataa aaattaggtg
      601 tcagtacttc ggccaaaggg acaagctttt ctttcaagct ttgcaaagca gattcttcta
      661 gtaaaagatt tccatgtttc gttacgatta cgggatccac gactaatggt ccaaaatcat
      721 atttttggta attttttaca actgtctcaa tcaactttga atctgctagc atacctgttt
      781 tagctgcacg tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg ataaaatctg
      841 ttggcaatgt cacacttgct ccgattccgt aagaattacc tgcaacacat gcggtcatga
      901 cagaaacacc gtaaacattt cttgtaaaga atgtatgcat atctgcttgc atccctgcgc
      961 ttccatcgct atctgatcct gcgatcgtca ttgcttgtac atactgatct tccatcaaat
     1021 cgattcctcc atttttataa aaagattttt cataatactg ttactgtttc tatcatatgt
     1081 ttttcaaagt atccctcagt aaatatacca ctaccttttc ttggtaatca aatttatttg
     1141 aaaaaacatt tgcaataaat cagaaaatag t
//
LOCUS       DAJSNV010000236         1148 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSNV010000236 DAJSNV010000000
VERSION     DAJSNV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.244"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            155..>1148
                     /locus_tag="ON516_002936"
     CDS             155..>1148
                     /locus_tag="ON516_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCR3346334.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAK"
BASE COUNT          368 a          241 c          201 g          338 t
ORIGIN      
        1 ctgaataatt catactttta attaaaacca tgtgaaactg cctcacggca gcttacaatt
       61 actttttcat cttcacccgt taagtgatga aaaaagaacc cctttctgct atggttaaag
      121 tgactaaacc aaccaaaaga aaggagttct tttcatgact agcttacaca aaaaccaagt
      181 aaaattcaat tcaaatatca ctatttctca tacaggtggt caattatcga gtgattcggg
      241 tctcgtccta gtgaaagagc tgatgaacat ctttggtttt tctgaactgg ctaagcaaca
      301 cattcacatt gaagacgaac gagcgtattt tactcatgac aacttatcca tacttgagca
      361 gttcattatg caattaattg ctggttactc agctgattct gcagctaatc tcctgagaca
      421 agatcctgtg tttaaagctg ttttagatag gaaagaactc gcttctcaat cttcactttc
      481 ccgattttta gatcgactat ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga
      541 acttatcgat aaagcacgcc tcatccgtaa tgatacggaa ttaatcattg atttagattc
      601 gacccattca gatacatttg gtcaccaaga acaaacgaat tataatacac actaccaaac
      661 ctatggttac catccattgg tagcttttga cggattgact ggtgacttct taaaagctga
      721 actacgttca ggaaatcaat atacatcaaa aggcgtaaag gagtttctca cacctttatt
      781 agagcactat aatcactctc taccaaacac tgacatcttg gttcgtggag acagcgggtt
      841 cgctacacct ggtgtgtatg attcgtgtga atcaaaaaag agtcattatg ttattcgact
      901 gaagaataat cgtagactag gtcaaatagc tgagaaatca gtactttatg gcgataatca
      961 aaagtgggaa gaacgagaag ttcagtactt ctcgaccact tatcaagcac aatcctggtc
     1021 acaaagtcgt cgcgtgtgta tacgctcaac acgtgaagcg ggcgaactac tctttcgaca
     1081 tgaatttatc gtgacgaatc tatcagaaaa tgtttctcct gataccatct tttctatcta
     1141 tgccaaac
//
LOCUS       DAJSNV010000237         1137 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSNV010000237 DAJSNV010000000
VERSION     DAJSNV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.245"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..355
                     /locus_tag="ON516_002937"
     CDS             <1..355
                     /locus_tag="ON516_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346335.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ON516_002938"
                     /pseudo
     CDS             615..910
                     /locus_tag="ON516_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ON516_002939"
     CDS             985..>1137
                     /locus_tag="ON516_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3346336.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAJSNV010000238         1114 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSNV010000238 DAJSNV010000000
VERSION     DAJSNV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.246"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            20..>1114
                     /locus_tag="ON516_002940"
     rRNA            20..>1114
                     /locus_tag="ON516_002940"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          342 a          219 c          326 g          227 t
ORIGIN      
        1 ccaaccgtaa ggttgataag gttaagtgaa taagggcgca cggtggatgc cttggcacta
       61 ggagccgatg aaggacggga ctaacaccga tatgctttgg ggagctgtac gtaagctatg
      121 atccagagat ttccgaatgg gggaacccaa catctttaat aggatgttac gattgtgtga
      181 atacatagca cattcgaggt agacgcagag aactgaaaca tctaagtacc tgcaggaaga
      241 gaaagaaaat tcgattccct gagtagcggc gagcgaaacg ggaaaagccc aaaccagcaa
      301 gcttgcttgt tggggttgta ggactccaat atggtagttc tttcagatag tcgaatgact
      361 tggaaaagtc agtcaaagag ggtaaaaacc ccgtagacga aatgtggaag acacctagga
      421 ggatcctgag tacggcggaa cacgagaaat tccgtcggaa tccgggagga ccatctccca
      481 aggctaaata ctccctagtg accgatagtg aaccagtacc gtgagggaaa ggtgaaaagc
      541 accccggaag gggagtgaaa tagaacctga aaccgtgtgc ctacaacaag tcaaagcccg
      601 ttaatgggtg atggcgtgcc ttttgtagaa tgaaccggcg agttacgatt gcatgcgagg
      661 ttaagttgaa gagacggagc cgcagcgaaa gcgagtctga atagggcgtt tgagtatgta
      721 gtcgtagacc cgaaaccatg tgatctaccc atgtccaggt tgaaggtgcg gtaaaacgca
      781 ctggaggacc gaacccacgt acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa
      841 attccaaacg aacttggaga tagctggttc tctccgaaat agctttaggg ctagcctcgg
      901 aattgagaat gatggaggta gagcactgtt tggactaggg gcccatctcg ggttaccgaa
      961 ttcagataaa ctccgaatgc cattcattca tatccgggag tcagactgtg agtgataaga
     1021 tccatagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat atatgttaag
     1081 tggaaaagga tgtggggttg cacagacaac tagg
//
LOCUS       DAJSNV010000239         1089 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSNV010000239 DAJSNV010000000
VERSION     DAJSNV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.247"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            66..851
                     /locus_tag="ON516_002941"
     CDS             66..851
                     /locus_tag="ON516_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HCR3346337.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          427 a          163 c          177 g          322 t
ORIGIN      
        1 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
       61 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
      121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
      181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
      241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
      301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
      361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
      421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
      481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
      541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
      601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
      661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
      721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
      781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
      841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
      901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
      961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     1021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     1081 gtttaaaag
//
LOCUS       DAJSNV010000240         1082 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSNV010000240 DAJSNV010000000
VERSION     DAJSNV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.248"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(62..280)
                     /locus_tag="ON516_002942"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="ON516_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="ON516_002943"
     CDS             complement(546..740)
                     /locus_tag="ON516_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346338.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="ON516_002944"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ON516_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          376 a          185 c          156 g          365 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 at
//
LOCUS       DAJSNV010000241         1081 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSNV010000241 DAJSNV010000000
VERSION     DAJSNV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.249"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1081
                     /locus_tag="ON516_002945"
     CDS             <1..>1081
                     /locus_tag="ON516_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3346339.1"
                     /translation="LNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILA
                     TGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAI
                     RKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKI
                     VTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCA
                     TTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGL
                     NLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSG
                     LLSMFSLAIEEQITLDKLKMLDYLF"
BASE COUNT          420 a          128 c          185 g          348 t
ORIGIN      
        1 cactaaattc tgagataaaa aagttatttc tagagacaga agttgacaaa attgatattc
       61 aaaataaaaa gattagttgt gtaaaaaaag atggtacaag attctctcaa gaatatgaca
      121 aagttatatt agctacaggt tcaaagcaaa ttatgctaga tataccgggg aataatttag
      181 ctaatatttt ttcgataaag aattttccaa atgcaaaaga gatttttaaa gctttggatg
      241 atagtactat taaaaaagta ggaattattg gagcaggata tattggagtt gaaattgctg
      301 aagccataag aaaaagaggg aaagaagtat atcttttcga tgttgcagat agagtgttgt
      361 cgacttatta cgaccgtagt ttttcagata aagttgaaga aatactatca aaaaatggaa
      421 tacatctttg tctatcagaa agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa
      481 tagtcactac aagtggtacc caatactcta tggatatggt cgtacaagct ataggatttc
      541 tatctaatag tccaattggg gaaaaggaac tcttgagaga tgttagtgga gcatatctaa
      601 ctaatgaata ccagcaaaca aatataaaag atatatatgc tattggggat tgtgctacaa
      661 ctttttttac gtccattaat agaagtagtg ttgatttttc tatttctaat gctctaagaa
      721 cagcttatat tgcaactcaa catattaaca atcaagattt tacaccttct ggttcccaat
      781 ttacaaatgg cttttctata tttaattaca atttttacgc agcaggtttg aatttaaaaa
      841 cagcacgatt attaagtgag tctatagatt atatagaaat agaagatttc attagacctg
      901 cttttttgag aaaaaatagt cgtgtaaaat taagaattag ctttgaaaaa aatacaaaga
      961 gaatagtagg tgtacaactt tgtagtcaag aagatttatc tggactatta tcaatgtttt
     1021 ctcttgctat agaagaacaa attacattag acaaattgaa aatgttagat tatttatttc
     1081 a
//
LOCUS       DAJSNV010000242         1035 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSNV010000242 DAJSNV010000000
VERSION     DAJSNV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.250"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>1035)
                     /locus_tag="ON516_002946"
     rRNA            complement(<1..>1035)
                     /locus_tag="ON516_002946"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAJSNV010000243         1034 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSNV010000243 DAJSNV010000000
VERSION     DAJSNV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.251"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..617
                     /locus_tag="ON516_002947"
     CDS             <1..617
                     /locus_tag="ON516_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR3346340.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            634..>1034
                     /locus_tag="ON516_002948"
     CDS             634..>1034
                     /locus_tag="ON516_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346341.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYK"
BASE COUNT          379 a          113 c          187 g          355 t
ORIGIN      
        1 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
       61 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      121 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      181 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      241 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      301 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      361 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      421 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      481 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      541 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt
      601 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      661 gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt
      721 tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac
      781 aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat
      841 attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga tgctaaaata
      901 tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag
      961 ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta
     1021 aacaactaca agtt
//
LOCUS       DAJSNV010000244         1011 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSNV010000244 DAJSNV010000000
VERSION     DAJSNV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.253"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(189..737)
                     /gene="aac(6')"
                     /locus_tag="ON516_002949"
     CDS             complement(189..737)
                     /gene="aac(6')"
                     /locus_tag="ON516_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3346342.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          299 a          211 c          146 g          355 t
ORIGIN      
        1 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
       61 tggtacacgg tgttccaaaa gtagcttctt cggaaataag ttggaatttc acaaaaattc
      121 aagaagtaat tttcgtaaat tccttcttat ttcttgaagc tgattacttc tgtcacaacc
      181 tctttttatt attgagaatt tggtcgagga ataatcgttt ttgccatcca aatatccggt
      241 ttgtcccagc catttgcatt tggtaagaca cctacgattt tataacctaa tttttcatag
      301 aattcatacg gatgttcacg aaggttctgg atggaagcca ctttatcaaa tgtatgctca
      361 tacaggtccg tttgacttaa cgttgttcca tggtctaaat catccgtacc taaataaatc
      421 gtgattcctc ctctggaagc tacttctttt tctaagtaat tgactaatcg agtacctatt
      481 tggttctttc gtcgggagct ttctacaact aatggatgca attcccaacc tgtgataccg
      541 tattgaggga ttgcaccaat aaatcctact aactcatctt ggtctaccgc tgctaccgcg
      601 attcgttctg gattcatcat ttcttctact tcttctgccg agctgtctcc atattcttcc
      661 ggccaagtca gtctcagtaa atcagaaagc tgatctttca atactggatt attacggtca
      721 aattcactga ttatcatacg tcttccccct tgttcttgct tattctaaca gtccttttga
      781 aaaaacgaaa tgttctcgct caaaaatctt tagctaccaa tcagtatctc tattctttca
      841 ttatttatct tacttataaa ataaaaaaat tatacagaaa cactttattc aaaataggaa
      901 agatcaggaa agccatcaat tggaggaaaa aactctgttt gttcatcatg atatagatta
      961 tcttttgctt taaaaaaaac caattatgaa ttgacaaata aaacctgttt t
//
LOCUS       DAJSNV010000245          987 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSNV010000245 DAJSNV010000000
VERSION     DAJSNV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.254"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..846
                     /locus_tag="ON516_002950"
     CDS             <1..846
                     /locus_tag="ON516_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3346343.1"
                     /translation="MHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVSV
                     KKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMCR
                     FLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQSY
                     FEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAVR
                     DGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLYQ
                     KGSKE"
BASE COUNT          364 a          104 c          213 g          306 t
ORIGIN      
        1 atgcacatgt cgtacagagg gagttttagt aggaaatatg tgctgaataa gtggtttaga
       61 ttattaaata ttcctgtaat catccatctt catggatcgg aatttaaaaa atggtatgat
      121 agtgtttctg taaaaaaaca aaatcagatt cgcttgctgt taagagagtc tgattccacg
      181 attgtacttg gagaaaaatg gaagaagata attaaagaaa ttgagccaaa ttgtaaaact
      241 gttgttgtag aaaattctat tgtagttcca gaatatacca taaagtggaa ttcaaaaatg
      301 tgccgatttc tgtttatggg agttttgata aagcgaaaag gtgtacacga tttggtagaa
      361 gcagttaaaa acattgcaga acaatatcca gaaaaaaagt ttatttttga gattgctgga
      421 gcaggtgatg aagaaatatc gctcaaagaa ttgatagaag aatataattt gcaatcgtat
      481 tttgaattta caggttgggt tgtgggagaa aaaaaggagg agtgctatag aagatcaaat
      541 gtgatgattt taccgtccta taacgaggga ttgcctatat ctattctaga ggcgatttct
      601 tatggaatgc ctgtaattgc tacagatgta ggagatattt cttcagcagt acgtgatgga
      661 gaaaatggat atttgtttca acctggtgac gtatcagcgt tgacatctgc aatactaaaa
      721 atcaatgata aagataaata cgtaaaattt tcacgttgtt cgaaggattt ggcaataacg
      781 aaatttaacg atggaaattt ctttgataga attttaaatc tgtatcaaaa agggagcaag
      841 gagtgatgac aaggaaaata aaaaatgttt cgattatgta atgaacttat taagtgaata
      901 aaatagagag aaaaaatatt gaaatagacg atgtaaattt ttatccgtgt aagaattcaa
      961 atgaatagta gtgttttagt gcttgta
//
LOCUS       DAJSNV010000246          984 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSNV010000246 DAJSNV010000000
VERSION     DAJSNV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.255"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..851
                     /locus_tag="ON516_002951"
     CDS             <1..851
                     /locus_tag="ON516_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3346344.1"
                     /translation="LKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKS
                     QNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEA
                     KVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRN
                     KLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLV
                     LADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMES
                     IYSRDH"
BASE COUNT          379 a          112 c          186 g          307 t
ORIGIN      
        1 tactaaaaaa agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta
       61 atttatggtt agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg
      121 aagatggaaa atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt
      181 tgttacaaaa tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc
      241 aaaaccatcc ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt
      301 cagaagctaa ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg
      361 gaaacattgg acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag
      421 aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa
      481 ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag
      541 aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt
      601 atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag
      661 cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg
      721 aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac
      781 aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta
      841 gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt
      901 gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa
      961 tgcagttttt attattcata taga
//
LOCUS       DAJSNV010000247          977 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSNV010000247 DAJSNV010000000
VERSION     DAJSNV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.256"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            346..977
                     /locus_tag="ON516_002952"
                     /pseudo
     CDS             346..977
                     /locus_tag="ON516_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DAJSNV010000248          936 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSNV010000248 DAJSNV010000000
VERSION     DAJSNV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.257"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..278)
                     /locus_tag="ON516_002953"
     CDS             complement(<1..278)
                     /locus_tag="ON516_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3346345.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPA"
     gene            complement(278..>936)
                     /locus_tag="ON516_002954"
     CDS             complement(278..>936)
                     /locus_tag="ON516_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3346346.1"
                     /translation="KKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDW
                     HPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSF
                     TLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDI
                     QLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN
                     "
BASE COUNT          313 a          159 c          104 g          360 t
ORIGIN      
        1 aaagcaggtt tttctaaaaa tatgtaatca actttttcaa caattacatt tagtgtttcc
       61 agatgcttaa aagtcggatt tgtaataaaa gctatatcat aatgtgtatc caaatcgtct
      121 ctattttgaa tatacgttat ttcttcaaac tctttttttc cactgctact ataagctgtt
      181 atatcaaaat taatagaatt cttttttcca tagcttatta gattttttag atgtctcttt
      241 ccaattgacc ctaacccaaa aaaaattact ttcatcatta atttacctca atgtcgttaa
      301 ttaagttaat aatatactta tctttttcaa aagtatattg gggttccatt ttcttttcca
      361 aaagcgtgaa aaattcttta atctcctcta aatatgcatc ttcaataata gtatgtgaat
      421 aattattatc atgcataacc tcatcgtaca attgaatatc tgtcatacta gaagtttctt
      481 cattccatga aaatagagaa tccgggttac cttcccattc tattaaaatc ttttcaccaa
      541 ttatagttaa atttctttta gctactcttg aaacaacatc tacacttaaa gttccaacta
      601 taccagtttc atgctcaata ataagagtga aactgtccga ataatcaata tcaagattgg
      661 ataatttctg ggtcattaca gtaaacgatt taactttccc aaatgtatct ataatccaag
      721 gtaattcaat agcgaatatt tctctacacc cattagtttt tttgtttgaa acaaaaaaat
      781 tgcgataatc ttcccaagga tgccagtcgg gtaaatactg tcctacatgg tattgataag
      841 aaattttttt attatgcgtc actttagact taatatattg aatttctttt ctcttcaaaa
      901 atgtagaaga tagataaagt aatctatctt tctttt
//
LOCUS       DAJSNV010000249          936 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSNV010000249 DAJSNV010000000
VERSION     DAJSNV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.258"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..818
                     /locus_tag="ON516_002955"
     CDS             <1..818
                     /locus_tag="ON516_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3346347.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          163 g          257 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catctt
//
LOCUS       DAJSNV010000250          926 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSNV010000250 DAJSNV010000000
VERSION     DAJSNV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.259"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(19..369)
                     /locus_tag="ON516_002956"
     CDS             complement(19..369)
                     /locus_tag="ON516_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3346348.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(363..626)
                     /locus_tag="ON516_002957"
     CDS             complement(363..626)
                     /locus_tag="ON516_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR3346349.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          301 a          161 c          110 g          354 t
ORIGIN      
        1 catgctgctg tttctatttt atttgcttac tttttgaaaa actgttctaa ctttcatcaa
       61 taactcctca actaaactat catgcgcttt ttccaaaaaa atacactctc tggcttcata
      121 atcaattgtc gttagttgat ctaataatac ttttcctgtt gtcttcattt catattctga
      181 gtttatattt acatatagcg gatagtttct agttgtacta gaaattggag caactgtaac
      241 aacgttacta taatccgcaa taatatcatg acttaaaaca atataaggcc ttttacctgt
      301 ctgttcgtgt ccagatcgag gtgctgtatt caataataga atatcgcctt gtctaggttt
      361 tctcaccatt tttcattacc tactgcttca cctaaatttt gaatcgtcac atgatttctt
      421 tcatcagtgt aattttcaaa taactgttca attgttaatt cattttctat attagccgct
      481 tcgataatta gccgcttctg accgtttact tcttcttgta ttatcttcac tttgtcttct
      541 gtatgaattc caagatattc cataatatac tttggcaatc taaggccatt actatttccc
      601 cattttttta taggtaacat ttccatagta gacacctctt tctgtatata catagtataa
      661 catacataag aaaaaacgta tatacttaat tatccttttc taataaacta tgttaacatt
      721 tatatgaaaa tagtcgaaag gaaatcataa tatgtttaag aaaacaaaat ctttagaatc
      781 aggtaagaaa aaaattgatc taaaaataat ctcttctgga tactttaaag atttcatagt
      841 tattgataca aaatggaata aaaaggacat agaaaatatt ctaaatagta ggaacaatta
      901 agtgctatca cttttgtttc ttcaaa
//
LOCUS       DAJSNV010000251          907 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSNV010000251 DAJSNV010000000
VERSION     DAJSNV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.260"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..338
                     /locus_tag="ON516_002958"
     CDS             <1..338
                     /locus_tag="ON516_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3346350.1"
                     /translation="EKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFF
                     QPLQYLEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSV
                     TIFFLSILI"
     gene            349..>907
                     /locus_tag="ON516_002959"
     CDS             349..>907
                     /locus_tag="ON516_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3346351.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCD"
BASE COUNT          320 a          126 c          152 g          309 t
ORIGIN      
        1 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
       61 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
      121 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
      181 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
      241 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
      301 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
      361 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
      421 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
      481 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
      541 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
      601 ggctacgtaa gtcaagatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
      661 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
      721 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
      781 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
      841 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
      901 tgtgatt
//
LOCUS       DAJSNV010000252          897 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSNV010000252 DAJSNV010000000
VERSION     DAJSNV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.261"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..638)
                     /locus_tag="ON516_002960"
     CDS             complement(<1..638)
                     /locus_tag="ON516_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3346352.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKIT"
BASE COUNT          295 a          166 c           97 g          339 t
ORIGIN      
        1 gttatttttg aatgttgcaa atatgaaata tggaatatga tatcttcaga tatataatcc
       61 ctttctgacg gaaacttgat attgtgctgt tgaattatct cagatgaata cagcgcatgc
      121 caaactgaca ttggcaatat aaaatcgact ttataactcg gctcagtgcc aatcattcca
      181 agcaacactt tttcaattat agcgttattt gtaaatatcg tactatcata tcttgctggc
      241 tttgaaataa tattattatc atcataatat tcataatatc cagcataaca tgtgtctgca
      301 ttactccttt ttataccatt taacattgct tcaatataat tagctgcaac aaaatcatca
      361 gaatcaataa aagcaacata ttttcctttt acatgttcaa gtcctgtatt tcttgccaat
      421 cccaagccag cattattttt atgaataata cgaatccttg aatcctttaa tgcatagtct
      481 tcacatattt tattagatga atccttagat ccatcatcca caattataat atctaggttc
      541 ttatatgttt gattacacac tgattccaaa caacttccta catacttttc aacattatat
      601 acaggtatga ttacactaat tagatcactc gaattcatcc ttctatcctt tcactcacgt
      661 tttctcaatt agtcaatgac ggcatcaacg attttcttaa attctctgaa aaaaatagac
      721 tctgatcttg atgatgtgaa tgacatacta tcttcttcta gaaaaattat ttcctttaaa
      781 ttatttatta aatcaataat atgatcctcg taagtatgct ttcccttgga ataatgaaaa
      841 tatacttttg tattacacaa tattgcaatt ttatttctta atatgattat ttcactc
//
LOCUS       DAJSNV010000253          890 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSNV010000253 DAJSNV010000000
VERSION     DAJSNV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.262"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..888
                     /locus_tag="ON516_002961"
     CDS             <1..888
                     /locus_tag="ON516_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3346353.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAJSNV010000254          880 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSNV010000254 DAJSNV010000000
VERSION     DAJSNV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.263"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..734)
                     /locus_tag="ON516_002962"
     CDS             complement(<1..734)
                     /locus_tag="ON516_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3346354.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGT"
     gene            complement(734..871)
                     /locus_tag="ON516_002963"
     CDS             complement(734..871)
                     /locus_tag="ON516_002963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346355.1"
                     /translation="MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGG
                     K"
BASE COUNT          261 a          202 c          153 g          264 t
ORIGIN      
        1 gtgccgttgt tattgccacc ttcaccccaa tgtttgatgg cgaattcatc atattcggtt
       61 aatttattag aagtgataat tgacatacaa cctgtataat acggactatc ttccgtaatt
      121 cctgcagcgg cataaccgcc tcgtcctaag atcaataagc ttgcgccagg gcttttattt
      181 cctacacaag cattaaaatt ggattgtgtg gctaccataa agccgtaata ggaaaatgca
      241 gcttttgagt tgggaaactt catgtaccag ccaccttcaa tgccaccaat tcctcgtgca
      301 gttcctttag gaaacagaca accatcaaac caagtaatcc caaaatagtt gagatcattt
      361 cttgcggacg ccgatgtacc aaacgctgat tcgtaaccgt attgcgaaag gatcacactt
      421 ggtaataatt tatatttcac tgctgcacct acggcatatt gcgcaatctc atcagaaaaa
      481 gggcctcttc ctttgatttc tttataaatc ggtaagcctt ccgaatattc tcctgtaaaa
      541 gcttcgccac cactgcttga atctgaatcc gaattatcaa tcgagcccgc cgcaagtatt
      601 agaaataggg tcacgaacac agtgatcaaa atagtaatca aacctgcaga agtaccaaac
      661 aaccacaaaa ataagcgccg ctttgcacga cgcttaacat actgcttgat taaactttca
      721 ttttggtttt ccattatttt cctccatcaa acaattttaa ttgacgtttg ttcggactaa
      781 aatgaacacg aatggtttct tttgagccaa aattgatcac agcgtctcct tgattttgtt
      841 gaggaatgga ttctaattct gagttcgtca ttgatttacc
//
LOCUS       DAJSNV010000255          874 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSNV010000255 DAJSNV010000000
VERSION     DAJSNV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.264"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            153..539
                     /locus_tag="ON516_002964"
     CDS             153..539
                     /locus_tag="ON516_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3346356.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            633..824
                     /locus_tag="ON516_002965"
     CDS             633..824
                     /locus_tag="ON516_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346357.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          322 a          135 c          141 g          276 t
ORIGIN      
        1 actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata
       61 gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt
      121 ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg
      181 cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa
      241 aacaagaaga aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta
      301 aagatactat aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag
      361 atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg
      421 aaaaatttat ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac
      481 agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat
      541 atctgcataa taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat
      601 agattataag tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt
      661 tagcaaaggc tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga
      721 ttcataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc
      781 ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc
      841 tgtgacataa gtaaaaagat agtttcaaat ttcg
//
LOCUS       DAJSNV010000256          870 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSNV010000256 DAJSNV010000000
VERSION     DAJSNV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.265"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..478
                     /locus_tag="ON516_002966"
     CDS             <1..478
                     /locus_tag="ON516_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346358.1"
                     /translation="VFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKL
                     HPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIM
                     LAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            465..>870
                     /locus_tag="ON516_002967"
     CDS             465..>870
                     /locus_tag="ON516_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3346359.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVI"
BASE COUNT          288 a          116 c          128 g          338 t
ORIGIN      
        1 agtttttgtc ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg
       61 catagatgaa acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac
      121 tttaattaag cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa
      181 ttccagttta ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat
      241 tagtcctata tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc
      301 tattatgttg gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga
      361 aacattgaca aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat
      421 tttactccca gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca
      481 cactttccaa gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta
      541 ttaagggaat ttcattcata actattccta tttttgtaaa tattactact caagcagaat
      601 atggtttgat taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa
      661 gcctaaattc ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta
      721 tgtcatcggt actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca
      781 tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt
      841 tattacaaag ttttgctagt tttgtaatta
//
LOCUS       DAJSNV010000257          862 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSNV010000257 DAJSNV010000000
VERSION     DAJSNV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.266"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(13..>862)
                     /locus_tag="ON516_002968"
     CDS             complement(13..>862)
                     /locus_tag="ON516_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3346360.1"
                     /translation="LISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFL
                     VLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDR
                     KVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETD
                     FYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIF
                     SLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYL
                     YLKKEN"
BASE COUNT          331 a          148 c          101 g          282 t
ORIGIN      
        1 gctgtcttca tattaattct ccttttttaa atacaagtaa gaaatgcttc ccaaataata
       61 ccagaaaaca attgaaatga catcgcgctt attgaaaagt acatgagcct ccaccatttc
      121 atctataaaa atagatagta taaaaattaa aacaatacca agaaacgcat aattcacttt
      181 acttctaaaa aatgttttga gtaaactaaa gatatttaac acatagaaag catagataca
      241 tataaaagca attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc
      301 atgtgctctt tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt
      361 tgcatcgcta ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat
      421 aactttaagc gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact
      481 agaatgctgt aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc
      541 tataacttct acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata
      601 attagtacca aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat
      661 ttttacaggt agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag
      721 aaataatact aaaaaagaac aaattactac ctgggcacct ctagacgaag aaagtgtaat
      781 ataaaatatt tgtacgataa aattcatcat ataaaaaaca cgaatcgttt ttgttatttt
      841 tgaaattaat acactaatta aa
//
LOCUS       DAJSNV010000258          807 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJSNV010000258 DAJSNV010000000
VERSION     DAJSNV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.267"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            75..308
                     /locus_tag="ON516_002969"
     CDS             75..308
                     /locus_tag="ON516_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR3346361.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            305..712
                     /locus_tag="ON516_002970"
     CDS             305..712
                     /locus_tag="ON516_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3346362.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          304 a          115 c          147 g          241 t
ORIGIN      
        1 gagagggact atgcttgatt taaaatcaac aagcgtatat actgtatata caaagaaaaa
       61 ggagggagaa gactatggca attaaagaaa gaaaatttag ggaagtgggg aactcagtag
      121 tattaactat ttcaaaggag tttcttaaag aaaatgggtt aaatcccgga gatacggttt
      181 ttttagatga agaaaaactt cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa
      241 ttgatttagc aatatctcaa gcattaactg aatatgatac tacttttaga gagcttgtcg
      301 ataaatgagt attcattacc tagaagccaa agatattata aaaatgaatg taatccaaat
      361 aaaaaaatac tctcctaatg aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg
      421 tgtagggcaa cttcgagcct cagcttttgg tgaagaggtt tatccttcga tttatgaaaa
      481 agcagctatc ctacttatac aattgataaa gaaacatcct tttcacaatg caaataaacg
      541 tactgctttc ttagctactt ttgtcttttt gaaaatcaat ggacagcttt tagctattga
      601 tcaaaaagat gctgtaaatt tagcggtata tattgctacg tatgataagg atttcgatca
      661 actaaaatat gaagtgatca acactataaa gacacatacg cagcccattt aaggagttac
      721 tgtatgtgtc tttatagggt tattagctgc tatagcgaaa taattgaagt aaattacaaa
      781 tagctataat tcaaatccaa ctatata
//
LOCUS       DAJSNV010000259          785 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSNV010000259 DAJSNV010000000
VERSION     DAJSNV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.268"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            365..>785
                     /locus_tag="ON516_002971"
     CDS             365..>785
                     /locus_tag="ON516_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3346363.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENST"
BASE COUNT          266 a          148 c          125 g          246 t
ORIGIN      
        1 taacaaaacc gaatgcaaaa cagaagttct ataagttata atcatagttg ttaggtgatt
       61 catctagtaa aaacaccaga tttcaaattt cacacaattt aaactagatc aatcatcaac
      121 cttcttcaaa ttgttagaac cctttcccga ggagctgcag cataagcaaa aaaacaaaca
      181 ggaagccaaa tggctaagac attttttcta ttagtctagt tgtttctcgt cgcaagttta
      241 agactttaga aagttatttc taatgatgtt tgggttgttt tgatcaatgc ttatgcacag
      301 ctccttacct attatttatc tcaacagatc taacaaatta atggactatt taaggagttt
      361 ttttatgctt acgatctatt cttctcgcac acatagttac catctgatgg caaactggct
      421 gaaagaccat caaatgcctt acagagaacg aattttcaca cctaaacagc ctttaacaag
      481 gaaagagata ttttatttat tgtcattaac agaaaacgga tttgacgatc tattagctaa
      541 acgctcgatt gattataaaa atttgaaaga ccaaatcgac caatgtacga cctcagagct
      601 tgtcgatctg atcgtctcgc attttacttt gttacaacgt cccatcatta ctgacggaaa
      661 gaaattatta gtcggattca atgaagatag catgcgtctt tttatccctg tacaatggcg
      721 cagaagtcaa ctgaaatctg tctataacga ttttacatgg gtaagcaatc tagaaaattc
      781 tacgg
//
LOCUS       DAJSNV010000260          764 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSNV010000260 DAJSNV010000000
VERSION     DAJSNV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.269"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(109..>764)
                     /locus_tag="ON516_002972"
     CDS             complement(109..>764)
                     /locus_tag="ON516_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR3346364.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          175 c          146 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccga
//
LOCUS       DAJSNV010000261          751 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSNV010000261 DAJSNV010000000
VERSION     DAJSNV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.270"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            99..272
                     /locus_tag="ON516_002973"
     CDS             99..272
                     /locus_tag="ON516_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346365.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILHLNNS"
     gene            complement(460..>751)
                     /locus_tag="ON516_002974"
     CDS             complement(460..>751)
                     /locus_tag="ON516_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346366.1"
                     /translation="AGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKG
                     MTIHSIRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          249 a          144 c          133 g          225 t
ORIGIN      
        1 gcaaaacatt tttaacgtaa gcgccccata gaacagtacc ttactaacaa atgcacctca
       61 agatatactt gagctatcaa gaagtctgga ggtcttctat gtataaatta aatgagattg
      121 ttcctgtcca attaggaaac gaaaagagtg caccagaaaa aggtaaaccc gcggctgcca
      181 agttagcgat gcgcatcaaa agagagaatg gttctgaagt tttgatctat aacggtatta
      241 acaattatat tcttcacctg aataattcat agctttaatt ctttggcaca ttttattgaa
      301 atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca
      361 ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc
      421 tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg
      481 cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt
      541 gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg
      601 aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt
      661 gactaagttg taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg
      721 actgtctgtt ttgtcaaagt aaaagccagc t
//
LOCUS       DAJSNV010000262          736 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSNV010000262 DAJSNV010000000
VERSION     DAJSNV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.271"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..320
                     /locus_tag="ON516_002975"
     CDS             <1..320
                     /locus_tag="ON516_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3346367.1"
                     /translation="DIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLE
                     TKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLEL
                     SKD"
     gene            392..>736
                     /locus_tag="ON516_002976"
     CDS             392..>736
                     /locus_tag="ON516_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346368.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          318 a           92 c          116 g          210 t
ORIGIN      
        1 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
       61 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      121 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      181 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      241 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
      301 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
      361 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
      421 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
      481 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
      541 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
      601 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
      661 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
      721 taatcttata caagat
//
LOCUS       DAJSNV010000263          713 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSNV010000263 DAJSNV010000000
VERSION     DAJSNV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.272"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            197..460
                     /locus_tag="ON516_002977"
     CDS             197..460
                     /locus_tag="ON516_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3346369.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..>713
                     /locus_tag="ON516_002978"
     CDS             460..>713
                     /locus_tag="ON516_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3346370.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          262 a           94 c          146 g          211 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gac
//
LOCUS       DAJSNV010000264          705 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSNV010000264 DAJSNV010000000
VERSION     DAJSNV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.273"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            108..>705
                     /locus_tag="ON516_002979"
     CDS             108..>705
                     /locus_tag="ON516_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346371.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDS"
BASE COUNT          251 a          125 c          143 g          186 t
ORIGIN      
        1 tatataaggt ataccctaac tagacacgac cgcgacattg aaaaatctgg ttttctcgtg
       61 tatgattagg gtgtttttta aatagttgca gaaagtaggt aaaaattttg agagttgcat
      121 acatacgtgt ctcatccatc gatcaaaatg aacaacggca gattgaagaa atgaaaaaat
      181 tcggtgcaga gagaattttc attgagaaac aatctggcgc aacgattact catcgacttg
      241 tttttcaaga agcactggat ttcgtccgag atcaagatat ttttattgtg gaagctattg
      301 atcgacttgg aagaaattac gacgagatca tcgcttcagt caattacttg aagaagaaaa
      361 atgtgcagct gatcatcaca agtctaccga tcatggccga agcaattggc aatcctctcc
      421 tggataaatt cattaaagat ttgatcattc aaattttggc catgatcgct gaacaagaac
      481 gtaccgaatc taaacgacgt caggcacaag gcattaagat tgcaaaagcg aacggtgttt
      541 ataaaggacg tcctaagcta tacagtgcca atgcaaaaga tccccaacgt cgtcttgttt
      601 ataaaaacat cgttgaggat ctaaaaaaag gtgttgcgat cgctaagatt gcaaaagact
      661 acaatgttac gagacaaacc gtatatagaa taaaaaaaga cagca
//
LOCUS       DAJSNV010000265          695 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSNV010000265 DAJSNV010000000
VERSION     DAJSNV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.274"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            132..344
                     /locus_tag="ON516_002980"
     CDS             132..344
                     /locus_tag="ON516_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346372.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>695
                     /locus_tag="ON516_002981"
     CDS             506..>695
                     /locus_tag="ON516_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3346373.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLD"
BASE COUNT          248 a          104 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agaca
//
LOCUS       DAJSNV010000266          693 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSNV010000266 DAJSNV010000000
VERSION     DAJSNV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.275"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..257
                     /locus_tag="ON516_002982"
     CDS             <1..257
                     /locus_tag="ON516_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3346374.1"
                     /translation="IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPE
                     SANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            258..>693
                     /locus_tag="ON516_002983"
     CDS             258..>693
                     /locus_tag="ON516_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3346375.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          265 a           78 c          125 g          225 t
ORIGIN      
        1 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
       61 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
      121 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
      181 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
      241 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
      301 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
      361 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
      421 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
      481 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
      541 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
      601 cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag
      661 ataaagacgt ctacttttca gttgtttctg caa
//
LOCUS       DAJSNV010000267          672 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSNV010000267 DAJSNV010000000
VERSION     DAJSNV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.276"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..>672
                     /locus_tag="ON516_002984"
     CDS             51..>672
                     /locus_tag="ON516_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3346376.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEG"
BASE COUNT          248 a          111 c          134 g          179 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg ca
//
LOCUS       DAJSNV010000268          637 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJSNV010000268 DAJSNV010000000
VERSION     DAJSNV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.277"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            7..624
                     /locus_tag="ON516_002985"
     CDS             7..624
                     /locus_tag="ON516_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346377.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          235 a           83 c          120 g          199 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataa
//
LOCUS       DAJSNV010000269          636 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSNV010000269 DAJSNV010000000
VERSION     DAJSNV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.278"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            26..>636
                     /locus_tag="ON516_002986"
     CDS             26..>636
                     /locus_tag="ON516_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346378.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLA"
BASE COUNT          196 a           95 c           86 g          259 t
ORIGIN      
        1 atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca
       61 cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt
      121 tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt
      181 tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt atattgttac
      241 gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac
      301 tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct
      361 tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt
      421 tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt
      481 attaatttac actcatgttc tttctgtagc tatgactatg gtaatgatta taattgcttt
      541 aattattcaa ttttatcgga aaaagaaaat agaaatcttc ttaattatta aattaataaa
      601 agctgctata gttactttac ttctttcact agcgtt
//
LOCUS       DAJSNV010000270          623 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSNV010000270 DAJSNV010000000
VERSION     DAJSNV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.279"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..450
                     /locus_tag="ON516_002987"
     CDS             <1..450
                     /locus_tag="ON516_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCR3346379.1"
                     /translation="PFPGSSLDKSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPG
                     SEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKV
                     GQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            466..>623
                     /locus_tag="ON516_002988"
     CDS             466..>623
                     /locus_tag="ON516_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346380.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKK"
BASE COUNT          201 a           97 c          155 g          170 t
ORIGIN      
        1 ccattccctg gcagcagttt agataaaagt tccttcagcg gtggtcaatt atttggaacg
       61 aatccaggcg gtgaattccg tccgaacggc ttccatgatg gattagattt tggttcagtt
      121 gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta cgttggcaat
      181 cctggtattt caggtttggg cgcgtgtgtg atcgtgatta attacgatgg cttgaatatg
      241 gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg cgatcaagtg
      301 aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg atttactcga
      361 atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg gatcgatccg
      421 ttaccatttt tgaatagcag taaaaaatag atcaggagaa aaaatatgga aaataaaacg
      481 ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat tgcgttgttt
      541 tggcagctga aagaggaaca agcagcaaat acctataaaa ttgaagaagt ggctaaaaac
      601 gcgtctgaga agctcaaaaa aaa
//
LOCUS       DAJSNV010000271          622 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSNV010000271 DAJSNV010000000
VERSION     DAJSNV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.280"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            62..>622
                     /locus_tag="ON516_002989"
     CDS             62..>622
                     /locus_tag="ON516_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346381.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLAT"
BASE COUNT          202 a           85 c          129 g          206 t
ORIGIN      
        1 aagaaatttc tgaaatgatt gggaaacggt tgttattgga atctgaaaag gtggtgaaag
       61 aatgatcatt ggaggaattt tttctggcat agccgactgg tggtcaggct tattcggaga
      121 aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa gattatggta attatctgca
      181 gtatcaagat gtatttagtt ggatttggaa cacttttctg tggggattga ttaaaggatt
      241 atataatctc aatactttac tagaaaaaac catttatcaa agctttgatt taaaagactt
      301 attgaacgcc gctggggtaa atgcactatt tcaaagtttg attagtaagg tattggctct
      361 tttttgtgtc gtcacattaa tttatttagg cttgaaattt tttatgagta aacacccacc
      421 aaaagtcaaa aatatcttgg ttaatttatt tatggccatg atcttgattt ttggtggtag
      481 ttctttaatc gatgaagggt taaatatcag taaaagcttt tacggagacg tcactgcagc
      541 aaataaagca gataaaacgg gatcacctgc ttttcaaatt attcaaaata atgtgatcga
      601 tgtcagtacg ttactagcaa ct
//
LOCUS       DAJSNV010000272          612 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSNV010000272 DAJSNV010000000
VERSION     DAJSNV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.281"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..146)
                     /locus_tag="ON516_002990"
     CDS             complement(<1..146)
                     /locus_tag="ON516_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346382.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ON516_002991"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ON516_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3346383.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          181 a          122 c          109 g          200 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gt
//
LOCUS       DAJSNV010000273          606 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSNV010000273 DAJSNV010000000
VERSION     DAJSNV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.282"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..418
                     /locus_tag="ON516_002992"
     CDS             <1..418
                     /locus_tag="ON516_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346384.1"
                     /translation="KRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAA
                     YNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDW
                     GNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(472..>606)
                     /locus_tag="ON516_002993"
     CDS             complement(472..>606)
                     /locus_tag="ON516_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3346385.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          207 a           93 c          113 g          193 t
ORIGIN      
        1 aaaacgcaaa gagttgcttc aagataaatt ttttcttttt ttcagttatt tattattatt
       61 gatcaatcac atacccattc aactgatttc tgaaccagta aagatcatca tcgatttttc
      121 ttatggtgct gcctataatc aatttatcaa gaaaaattta agcttgtaca ttaatctgaa
      181 cacggaagtg atagaggcag gaaagcctga gttcccggat gttataatca cgaataggaa
      241 taatctatat gaagaaagtg catctcaagt agtggtttgg ctcgatccgc caagagcagt
      301 agactggggg aatcttacaa agattttgtt gactatccaa gaagagaaat acaaagacag
      361 caaaattgac aagcaaatag acgattttcc tgaagaaatc atcgacagga tcgaatagtg
      421 aacttttttt agaaagtccg actgcttttt ctaaaaatat tatttgtcaa attaagcgag
      481 acaaaacttt ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga
      541 ttttctaggg atatggtttc ttttggacgc aacggatgag atgaatcctt gattgacttg
      601 gttgaa
//
LOCUS       DAJSNV010000274          599 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSNV010000274 DAJSNV010000000
VERSION     DAJSNV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.283"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..449
                     /locus_tag="ON516_002994"
     CDS             <1..449
                     /locus_tag="ON516_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HCR3346386.1"
                     /translation="QFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKPLNYSYKAFLNR
                     DEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDDHQLQLIRQFDE
                     MNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
BASE COUNT          230 a           65 c          125 g          179 t
ORIGIN      
        1 ttcaatttga tagattgtta aaagctattg atgatttaat tgaaaaagga gtaataatcg
       61 aaagtacatt tgcacagagt ggttatagtg attataaacc actaaattat tcctataaag
      121 catttctaaa tagggatgag tttactgaaa ctatgcaaaa agcagaaatg gtgattactc
      181 atggaggtac aggtgctata attggggccg taaaaaaagg taaaagggtc attgctgttc
      241 ctcgattggc taagtatggt gagcatgtcg atgatcacca actgcagtta attagacaat
      301 ttgatgagat gaatattatc tatccttgtt atgaattaga aaatttagaa gatgccgtta
      361 aagaaacaag agttcggatg tttaacaagt atgtatctaa cacggaaacg attattaatt
      421 cgatagaggt attaatagaa aaaatataaa tataaggagt aaagcgttat gcaaagagta
      481 cttatgattg gtaatgacag ctcggtaaaa ggtggaatta caagtataat tacacaaatg
      541 ctcaatttta attgggaaaa aaagaatatc gagatgactt ttataccgag ttataaggg
//
LOCUS       DAJSNV010000275          595 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSNV010000275 DAJSNV010000000
VERSION     DAJSNV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.284"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..580
                     /locus_tag="ON516_002995"
     CDS             <1..580
                     /locus_tag="ON516_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3346387.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          106 c          122 g          163 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cctct
//
LOCUS       DAJSNV010000276          593 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSNV010000276 DAJSNV010000000
VERSION     DAJSNV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.285"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(318..581)
                     /locus_tag="ON516_002996"
     CDS             complement(318..581)
                     /locus_tag="ON516_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HCR3346388.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DAJSNV010000277          584 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSNV010000277 DAJSNV010000000
VERSION     DAJSNV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.286"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>584
                     /locus_tag="ON516_002997"
     CDS             <1..>584
                     /locus_tag="ON516_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR3346389.1"
                     /translation="LYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRY
                     ALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNA
                     WVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLV
                     VPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTV"
BASE COUNT          183 a           71 c          114 g          216 t
ORIGIN      
        1 attatatttt gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt
       61 gataggttta gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa
      121 acagtggaga tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat
      181 agttttagca caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg
      241 aatatattcc tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa
      301 taacgcctgg gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa
      361 gaaagttagt aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt
      421 ttcgattgat ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt
      481 ggttccgata accttaggct atttctttca gtttttatat agtttaccag ttaatgctga
      541 attttatgag aaaaaaacat cgtttattgc gattggtaca gtag
//
LOCUS       DAJSNV010000278          528 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSNV010000278 DAJSNV010000000
VERSION     DAJSNV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.287"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..135)
                     /locus_tag="ON516_002998"
     CDS             complement(<1..135)
                     /locus_tag="ON516_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3346390.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     K"
     gene            complement(154..315)
                     /locus_tag="ON516_002999"
     CDS             complement(154..315)
                     /locus_tag="ON516_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3346391.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            385..>528
                     /locus_tag="ON516_003000"
     CDS             385..>528
                     /locus_tag="ON516_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3346392.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQA"
BASE COUNT          176 a          106 c           67 g          179 t
ORIGIN      
        1 cttttctatt tcattgcttg atttcattat tgttaaacaa gaatacaagc aagaaagaac
       61 tatagttaat atgataaaaa ttattaaagt tctatttttc tttcttgtaa tatatgctat
      121 agcatttttt atcactttcc taactactcc tctctatttt cttactccat aactaattgc
      181 atccactgga caagcatttt tacatcgtcc acatctgata cactccagat gattgcaatt
      241 ctcaactgaa tctttattca tttggcaaac ttttttacat tttccacaat taatacactt
      301 atccttatcc tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat
      361 aaaaaacaca ctggaggatt tttcatgcca agacgtcaac gaagaaccta ttcaaaagaa
      421 ttcaaacaac aaatcgtcga tctctatctc gctggtaagc ctcgcgcaga aattattcga
      481 gagtatgagc ttacgccttc ttctttcgat aaatggatga agcaagca
//
LOCUS       DAJSNV010000279          508 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJSNV010000279 DAJSNV010000000
VERSION     DAJSNV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.288"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAJSNV010000280          495 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSNV010000280 DAJSNV010000000
VERSION     DAJSNV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.289"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..269
                     /locus_tag="ON516_003001"
                     /pseudo
     CDS             1..269
                     /locus_tag="ON516_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAJSNV010000281          486 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSNV010000281 DAJSNV010000000
VERSION     DAJSNV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.290"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..387
                     /locus_tag="ON516_003002"
     CDS             <1..387
                     /locus_tag="ON516_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3346393.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          188 a           77 c           89 g          132 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatt
//
LOCUS       DAJSNV010000282          468 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSNV010000282 DAJSNV010000000
VERSION     DAJSNV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.291"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>468
                     /locus_tag="ON516_003003"
     CDS             <1..>468
                     /locus_tag="ON516_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3346394.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAJSNV010000283          467 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJSNV010000283 DAJSNV010000000
VERSION     DAJSNV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.292"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..464)
                     /locus_tag="ON516_003004"
     CDS             complement(<1..464)
                     /locus_tag="ON516_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346395.1"
                     /translation="MLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLF
                     FSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF
                     ENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFY"
BASE COUNT          159 a           74 c           66 g          168 t
ORIGIN      
        1 caataaaatg ctggatagtt ttctctaaaa aaagaaacat caagtggaaa ttcatcagct
       61 ttattaatag tgtaatgcaa aagtttaaaa tgtattttgg ttaactctct tcggatcaat
      121 tggctcgtct cttctcgcaa cagattaaac tgattttcaa aactttcaat gaatcgttca
      181 ttcaaccatt cgattgtttc atcttcattt tttctcaagt atttttgatc caatttatca
      241 tgaagataaa gccaagagag tgtcttattg ataaaaataa cggatttttc tcctaatact
      301 ccttttaagt ggtaaaatcc tatatccgaa aaaaatagcg actgattgct agcaatatct
      361 aaagaatcag cttgttcagc caacaggttg attgctaaaa agtggaataa tttctttttt
      421 tcataagcag acagaggata atactgtttt aaatctttta acatttt
//
LOCUS       DAJSNV010000284          460 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSNV010000284 DAJSNV010000000
VERSION     DAJSNV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.293"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            67..261
                     /locus_tag="ON516_003005"
     CDS             67..261
                     /locus_tag="ON516_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346396.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          195 a           62 c           80 g          123 t
ORIGIN      
        1 acataatcat tttcacttac atatatttga atattaacta aaaacagcaa gaaagaagag
       61 gatcaaatgt attatgtaga agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa
      121 ggtatcagta acgaatatcc tttacttggt tcatggaaag aagctgctcc tttttccaaa
      181 ccatgtgcca taaaaataaa gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt
      241 gtagatatca tcgaaaagta aatccaatgt atagaaagga agatcacaca tgaaaagcga
      301 cgtacaaatt gctaagatta tagaattaga agaagaagac gcagtgttga caattgaaaa
      361 aaaataaatg tttgtatacc atatgattat ttcaatatgt atcctggaat tggagatctc
      421 gtagagctat atcaagatga atcaaatata cttgtcattc
//
LOCUS       DAJSNV010000285          457 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSNV010000285 DAJSNV010000000
VERSION     DAJSNV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.294"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(79..228)
                     /locus_tag="ON516_003006"
     CDS             complement(79..228)
                     /locus_tag="ON516_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR3346397.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(221..430)
                     /locus_tag="ON516_003007"
     CDS             complement(221..430)
                     /locus_tag="ON516_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3346398.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          132 a           97 c           43 g          185 t
ORIGIN      
        1 taataaggta ggaataattt ataaaaaaac tcctcaaaca acatagtact ttgagaagtt
       61 tctgcttctt ttccataatt agaaacaagt tcggtatacg ctttttttct tctttaactg
      121 ttttaacccc tctacgtttt tttgtactct tttccctgtt cgtttgtcga atcccaaact
      181 agcagtaaaa tgatatttcc catttttatc ttttttaaaa ttaaccatga ttctgaaaat
      241 cctcctctac ctctatcttc atacctaaaa cttcttctac agattcaaca ggtactagtc
      301 ctaatcttct attttcgtaa taggtatagc tttttgaaac cataattgct tttgcctgtc
      361 gtatgattct ctcagactga tacttcccat aacctaattt cattaattca ttttttgtta
      421 taaccaccat ttaaccacca cattttttat tcaaata
//
LOCUS       DAJSNV010000286          443 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJSNV010000286 DAJSNV010000000
VERSION     DAJSNV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.295"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            22..>443
                     /locus_tag="ON516_003008"
     CDS             22..>443
                     /locus_tag="ON516_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3346399.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          160 a           84 c           91 g          108 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggc
//
LOCUS       DAJSNV010000287          436 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSNV010000287 DAJSNV010000000
VERSION     DAJSNV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.296"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            189..>436
                     /locus_tag="ON516_003009"
     CDS             189..>436
                     /locus_tag="ON516_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346400.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPN"
BASE COUNT          167 a           54 c           84 g          131 t
ORIGIN      
        1 taattggtgt aattaattgt agagaatagc ctttgagcaa ttgaagcgat acaattattt
       61 agaaaatgaa cttcctccag catactacat gattccttac gggacgatgt taaagaagaa
      121 agcaagaaat tttagaagta ttagtagata aactaatggg cttttctcaa tttaagggag
      181 tagcccaaat gtttatcaaa tatggtgcag atacagtagc aaccatgttt acagaagaat
      241 atcaagtaga ctattatgaa gtaatagata aatgtgtaca ttttgtttat gaaaaagtgt
      301 ggaattttat gaattttgcg ataagtgaag aaatcttacg agtacaagaa aacagaacag
      361 gaatatttac cagagacaga aagcttaatt tgattgcgca ttatatacgg aatcgctggt
      421 catcaatacc aaattt
//
LOCUS       DAJSNV010000288          421 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSNV010000288 DAJSNV010000000
VERSION     DAJSNV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.298"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>421
                     /locus_tag="ON516_003010"
     CDS             <1..>421
                     /locus_tag="ON516_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3346401.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          132 a           69 c           85 g          135 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 g
//
LOCUS       DAJSNV010000289          414 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSNV010000289 DAJSNV010000000
VERSION     DAJSNV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.299"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            217..>414
                     /locus_tag="ON516_003011"
     CDS             217..>414
                     /locus_tag="ON516_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346402.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVD"
BASE COUNT          155 a           47 c           88 g          124 t
ORIGIN      
        1 atcactttct cacgaaaggt gttaggtagg ctgtgcctac cacgtttcta agaaagataa
       61 atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg
      121 cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta
      181 cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat tagaaatatt
      241 gaagaaaagt atattaataa aattgacaaa cgttgtgaag aacttacggt caagactggc
      301 aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt tgatcacgct
      361 cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt ggat
//
LOCUS       DAJSNV010000290          414 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSNV010000290 DAJSNV010000000
VERSION     DAJSNV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.300"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>414
                     /locus_tag="ON516_003012"
     CDS             <1..>414
                     /locus_tag="ON516_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346403.1"
                     /translation="VFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIYNYHGWE
                     GLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEEGGASSL
                     ESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          162 a           54 c           82 g          116 t
ORIGIN      
        1 ttgtttttaa gaaaaaagta gtttcagact atttaaataa cgaaggcggc tacaaatatc
       61 ttgcacataa atatcaaata aatcgtacac tggttagaca ttgggtaagg atttataact
      121 atcatggttg ggaaggcttg gttggaggtg gcaaaagcta cactacaaaa tttaaacttg
      181 atgttataga atatatggaa acaaatggtc tttctatcca agaaactgct aaaaaattta
      241 atatcggttc aaatagaact ctaagtaaat ggatagagca atatgaagaa ggcggtgctt
      301 cttcacttga gagccaaaaa aggggcagaa aaattagtat gaattccaag ctaaacattc
      361 ctaaaaaact taaagatgag tctcttgaag aagaagttat tcgtttaaga gcag
//
LOCUS       DAJSNV010000291          404 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJSNV010000291 DAJSNV010000000
VERSION     DAJSNV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.301"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>404
                     /locus_tag="ON516_003013"
     CDS             <1..>404
                     /locus_tag="ON516_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346404.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSNV010000292          402 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJSNV010000292 DAJSNV010000000
VERSION     DAJSNV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.302"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..101
                     /locus_tag="ON516_003014"
     CDS             <1..101
                     /locus_tag="ON516_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3346405.1"
                     /translation="YQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            205..351
                     /locus_tag="ON516_003015"
     CDS             205..351
                     /locus_tag="ON516_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR3346406.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLY
                     YLSN"
BASE COUNT          166 a           40 c           72 g          124 t
ORIGIN      
        1 aatatcaaga ggaattaata agaaatttga gtgaaattgg aagaactgct ggaataaatg
       61 gtttatatcg agcagattat ggagataaat ctcaatttta atgatactta taatattaaa
      121 tatgtctcaa aattttttga ttagaaaaat aggtatattg aatcagtgac ataaaagtat
      181 ttatagtttg tagggggaat cgtaatggaa gaaaaaaata gactaaatgc taaacaatgt
      241 tcagatcaag agcttaaaaa aattaaagga ggtgctggaa caaaacctca aggaaaaccg
      301 gcatcaaatt tagtggaatg tgttttttca ttatattatt tgtcaaatta agcgagacaa
      361 aactttccat ttacgtaact caaaaaaact tctaatggtg tt
//
LOCUS       DAJSNV010000293          394 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJSNV010000293 DAJSNV010000000
VERSION     DAJSNV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.303"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..285
                     /locus_tag="ON516_003016"
     CDS             <1..285
                     /locus_tag="ON516_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3346407.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DAJSNV010000294          386 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSNV010000294 DAJSNV010000000
VERSION     DAJSNV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.304"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>386
                     /locus_tag="ON516_003017"
     CDS             <1..>386
                     /locus_tag="ON516_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346408.1"
                     /translation="PKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKD
                     SRLNQFKDNDQSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVT
                     REAAPKPIQNVHTGYKPANVAFTAPT"
BASE COUNT          145 a           71 c           74 g           96 t
ORIGIN      
        1 ccaaaacgaa atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat
       61 ggaatcgaag ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta
      121 ccaatgaaag atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa
      181 tcacctgcag gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca
      241 actgtttctt acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt
      301 gtgacaagag aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca
      361 aatgtggcat ttactgcacc aacaaa
//
LOCUS       DAJSNV010000295          376 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJSNV010000295 DAJSNV010000000
VERSION     DAJSNV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.305"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>376
                     /locus_tag="ON516_003018"
     CDS             82..>376
                     /locus_tag="ON516_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3346409.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          105 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccatt
//
LOCUS       DAJSNV010000296          363 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJSNV010000296 DAJSNV010000000
VERSION     DAJSNV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.denovo.306"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>363
                     /locus_tag="ON516_003019"
     CDS             <1..>363
                     /locus_tag="ON516_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HCR3346410.1"
                     /translation="LKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDID
                     IHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSLENETA
                     KKLLIQFYEEQAADRLQEY"
BASE COUNT          137 a           55 c           60 g          111 t
ORIGIN      
        1 ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt taatcatggg
       61 gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc gtgggatatc
      121 aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt acaaattgat
      181 tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact gcatgatcaa
      241 ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatctct tgagaatgag
      301 acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg acttcaagaa
      361 tat
//
LOCUS       DAJSNV010000297        50669 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.012, whole genome shotgun sequence.
ACCESSION   DAJSNV010000297 DAJSNV010000000
VERSION     DAJSNV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.012"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..622
                     /locus_tag="ON516_003020"
     CDS             <1..622
                     /locus_tag="ON516_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346411.1"
                     /translation="NPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPS
                     PTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVD
                     LMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYL
                     RAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            644..856
                     /locus_tag="ON516_003021"
     CDS             644..856
                     /locus_tag="ON516_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3346412.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            931..1407
                     /locus_tag="ON516_003022"
     CDS             931..1407
                     /locus_tag="ON516_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCR3346413.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            1404..1904
                     /locus_tag="ON516_003023"
     CDS             1404..1904
                     /locus_tag="ON516_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HCR3346414.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            2464..4281
                     /locus_tag="ON516_003024"
     CDS             2464..4281
                     /locus_tag="ON516_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3346415.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            4387..5715
                     /locus_tag="ON516_003025"
                     /pseudo
     CDS             4387..5715
                     /locus_tag="ON516_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            5792..5953
                     /locus_tag="ON516_003026"
     CDS             5792..5953
                     /locus_tag="ON516_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HCR3346416.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            5994..6374
                     /locus_tag="ON516_003027"
     CDS             5994..6374
                     /locus_tag="ON516_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HCR3346417.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            6389..6670
                     /locus_tag="ON516_003028"
     CDS             6389..6670
                     /locus_tag="ON516_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346418.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            6929..7711
                     /locus_tag="ON516_003029"
     CDS             6929..7711
                     /locus_tag="ON516_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR3346419.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            7884..9602
                     /locus_tag="ON516_003030"
     CDS             7884..9602
                     /locus_tag="ON516_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR3346420.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            9604..10458
                     /locus_tag="ON516_003031"
     CDS             9604..10458
                     /locus_tag="ON516_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3346421.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            11134..11454
                     /locus_tag="ON516_003032"
     CDS             11134..11454
                     /locus_tag="ON516_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346422.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            11478..11888
                     /locus_tag="ON516_003033"
     CDS             11478..11888
                     /locus_tag="ON516_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3346423.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            12035..12241
                     /locus_tag="ON516_003034"
     CDS             12035..12241
                     /locus_tag="ON516_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346424.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            12588..13109
                     /locus_tag="ON516_003035"
     CDS             12588..13109
                     /locus_tag="ON516_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3346425.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTRSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            13167..13592
                     /locus_tag="ON516_003036"
     CDS             13167..13592
                     /locus_tag="ON516_003036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3346426.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            13771..14001
                     /locus_tag="ON516_003037"
     CDS             13771..14001
                     /locus_tag="ON516_003037"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCR3346427.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            14035..14217
                     /locus_tag="ON516_003038"
     CDS             14035..14217
                     /locus_tag="ON516_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HCR3346428.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            14330..15841
                     /locus_tag="ON516_003039"
     CDS             14330..15841
                     /locus_tag="ON516_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346429.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            16164..16388
                     /locus_tag="ON516_003040"
     CDS             16164..16388
                     /locus_tag="ON516_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HCR3346430.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            16381..16947
                     /locus_tag="ON516_003041"
     CDS             16381..16947
                     /locus_tag="ON516_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HCR3346431.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            16935..17165
                     /locus_tag="ON516_003042"
     CDS             16935..17165
                     /locus_tag="ON516_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346432.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            17366..17731
                     /locus_tag="ON516_003043"
     CDS             17366..17731
                     /locus_tag="ON516_003043"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HCR3346433.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            17731..19458
                     /locus_tag="ON516_003044"
     CDS             17731..19458
                     /locus_tag="ON516_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR3346434.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            19488..21404
                     /locus_tag="ON516_003045"
     CDS             19488..21404
                     /locus_tag="ON516_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3346435.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            22089..22271
                     /locus_tag="ON516_003046"
     CDS             22089..22271
                     /locus_tag="ON516_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346436.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            22298..23995
                     /locus_tag="ON516_003047"
     CDS             22298..23995
                     /locus_tag="ON516_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3346437.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            24020..24151
                     /locus_tag="ON516_003048"
     CDS             24020..24151
                     /locus_tag="ON516_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346438.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            24243..25106
                     /locus_tag="ON516_003049"
     CDS             24243..25106
                     /locus_tag="ON516_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346439.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            25143..25679
                     /locus_tag="ON516_003050"
     CDS             25143..25679
                     /locus_tag="ON516_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCR3346440.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            25693..26115
                     /locus_tag="ON516_003051"
     CDS             25693..26115
                     /locus_tag="ON516_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3346441.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            26045..28444
                     /locus_tag="ON516_003052"
     CDS             26045..28444
                     /locus_tag="ON516_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCR3346442.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            28476..30467
                     /locus_tag="ON516_003053"
     CDS             28476..30467
                     /locus_tag="ON516_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3346443.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            30490..30741
                     /locus_tag="ON516_003054"
     CDS             30490..30741
                     /locus_tag="ON516_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCR3346444.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            30731..31960
                     /locus_tag="ON516_003055"
     CDS             30731..31960
                     /locus_tag="ON516_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCR3346445.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            31957..34038
                     /locus_tag="ON516_003056"
     CDS             31957..34038
                     /locus_tag="ON516_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR3346446.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            34187..38107
                     /locus_tag="ON516_003057"
     CDS             34187..38107
                     /locus_tag="ON516_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCR3346447.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            38108..39052
                     /locus_tag="ON516_003058"
     CDS             38108..39052
                     /locus_tag="ON516_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346448.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            39056..39259
                     /locus_tag="ON516_003059"
     CDS             39056..39259
                     /locus_tag="ON516_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCR3346449.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(39559..39795)
                     /locus_tag="ON516_003060"
     CDS             complement(39559..39795)
                     /locus_tag="ON516_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346450.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(39978..40364)
                     /locus_tag="ON516_003061"
     CDS             complement(39978..40364)
                     /locus_tag="ON516_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCR3346451.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(40346..41674)
                     /locus_tag="ON516_003062"
     CDS             complement(40346..41674)
                     /locus_tag="ON516_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3346452.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(41635..41964)
                     /gene="mobC"
                     /locus_tag="ON516_003063"
     CDS             complement(41635..41964)
                     /gene="mobC"
                     /locus_tag="ON516_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCR3346453.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(42222..42593)
                     /locus_tag="ON516_003064"
     CDS             complement(42222..42593)
                     /locus_tag="ON516_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3346454.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            42738..42923
                     /locus_tag="ON516_003065"
     CDS             42738..42923
                     /locus_tag="ON516_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3346455.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            43081..43248
                     /locus_tag="ON516_003066"
     CDS             43081..43248
                     /locus_tag="ON516_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346456.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            43866..44525
                     /gene="vanR"
                     /locus_tag="ON516_003067"
     CDS             43866..44525
                     /gene="vanR"
                     /locus_tag="ON516_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCR3346457.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            44528..45871
                     /gene="vanS"
                     /locus_tag="ON516_003068"
     CDS             44528..45871
                     /gene="vanS"
                     /locus_tag="ON516_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3346458.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            46042..46848
                     /gene="vanY"
                     /locus_tag="ON516_003069"
     CDS             46042..46848
                     /gene="vanY"
                     /locus_tag="ON516_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCR3346459.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGGCLEEYLKPEK"
     gene            46866..47693
                     /gene="vanW"
                     /locus_tag="ON516_003070"
     CDS             46866..47693
                     /gene="vanW"
                     /locus_tag="ON516_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCR3346460.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            47690..48661
                     /locus_tag="ON516_003071"
     CDS             47690..48661
                     /locus_tag="ON516_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCR3346461.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            48654..49682
                     /gene="vanB"
                     /locus_tag="ON516_003072"
     CDS             48654..49682
                     /gene="vanB"
                     /locus_tag="ON516_003072"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCR3346462.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            49688..50296
                     /locus_tag="ON516_003073"
     CDS             49688..50296
                     /locus_tag="ON516_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCR3346463.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(50362..50535)
                     /locus_tag="ON516_003074"
     CDS             complement(50362..50535)
                     /locus_tag="ON516_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346464.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        14564 a        11218 c        13294 g        11593 t
ORIGIN      
        1 aaatccgaca tcggataatc cggcgtcgga aaatccaacg caattaaata aagaagtaca
       61 aagaactgac ttatcaaaaa aagaaaaaat aattacagat ggacaaagta cccattccat
      121 tcctatccca tccccgaccc ctgcccctat ggagggcgag gcagcggcta cgccgccgga
      181 acggaaaagg acaggaaagg atgaggctgt acggatttat cgggaaatca ttttggagaa
      241 catcgagtat gcctatctca ttcaggacag gagcattgac cgtgaacagc ttgacgaaat
      301 cgtagacctg atgctggaaa ccgtctgcac cgcccgaaag acaatccgtg tcgccgggga
      361 cgactaccct gccgagctgg tgaaatccaa gtacatgaag ctgaccggcg aacatatccg
      421 ctttgtcctt gactgtctgc gggagaacac aacgaaagtc cgcaatatca aacaatacct
      481 gcgggctatg ctgttcaacg cccccagcac catttccaac tactacacgg cccttgtcgc
      541 gcacgacatg gcacagcccg attggggaaa gcctaaatcc ggcctgccgg actattcctg
      601 cggcgagggc gaaagcctgt aacgagaaaa ggaggacgac cctatgaagc agggcgcatt
      661 gatttttgac gagcgaacag accgttacga catccgcttt gatctggccg actattacgg
      721 cggcctgcat tgcggggagt gtttcgacgt gatgatcggc ggcaaatgga ggcccacccg
      781 cattgaaatg gccgaaaact ggtatcttgt cggcatccgg gccgacgacc tttccggcct
      841 gcgggtgcgg atttgaccta tatctacctg cggcgtctgg tggttccgcc ttagacgccg
      901 cttttgactt tctgacaagg agggatgcga ttgcaggaag aagttactca aaagaccatt
      961 gccctgtcca tgaaaacggg caagctcacg gctcaagtgc tgcaagcggc gctgaaaaag
     1021 tacctgcaac accgggccaa gggcaaaaac acgctgcacc acggccagca gagcctaaag
     1081 cagcttaaaa agcatggggc tgatctctcc aacattgaga tcaccgaggc caacattgga
     1141 gcgttcaagc catgcgccaa gaaatacggc gtggatttta ccttacgaaa agataagacc
     1201 acccagccgc cccactacgt tgtgattttc aaggccaagg atgcagataa ccttgatctg
     1261 gcgtttcggg agtttaccgc caagacgctt tccaaggagc agcggccctc tatccgcaag
     1321 gttttgtccg ctgtgaagca aaaggcggcg gcccagacca agcagcgggc caaggagaaa
     1381 atcaaggaaa gggggctgga actgtgaagc ctgacgtaaa gaaattgctg atcctgaacc
     1441 tcccgtatct gatttttgtg tacctgtttg cgaaatgcgg ggaggcgtac cggctggccg
     1501 ctggtgctga cctgtcgggg aagctcctgc attttgcgga tggttttgcg gcggcttttg
     1561 ccaacccgct gccaagcctg cacctgtttg acctgtgtat cggtatcgcc ggtgccgtga
     1621 tcgtgcggct ggtggtgtac tgcaagagca agaacgtgaa gaaataccgc aaaggcatgg
     1681 agtatggcag cgcccgctgg gggacggcca aagacatagc gccttacatc gaccccaagt
     1741 ttgaaaataa tatcctgctc acccaaacgg aacggcttac catgaccggc aggcccaaag
     1801 accccaagac agcgcggaat aaaaacgtgc tggtgatcgg tggcagcggt tcagggaaaa
     1861 cgagattttt cgtgaaaccc agtgcgcccg taagggcggt ataaatccta ccttgagcaa
     1921 gtagtaggta aaagtatcaa gcggcattgt gccgcaacct atcattcccc gtcttacccc
     1981 aaaagggaaa ccggagggcg accatagcat gacggaggac acggggcgct gaagcctcag
     2041 aagcgccggc gtgacggaat gaaaatccac gtatgaggat ggaagctaga ctgcttgaat
     2101 gacagcctga aatcgggacc aagagcgtac ccctgacgta gagaggttgt actcgtcagt
     2161 gttcctgaca gtcgcattgt ttggctatgc ggctgctgat actcggagca ggttcagcca
     2221 cgggaaagtg gaaaaaggcg aacgtcacat accgacaacg tggaacgctt gtttataccg
     2281 tactaaacgg ggattgccta aagtgaaatg ccgaaaggct atgaaaacgc tgaaatgcgg
     2341 ggttctgaat attcaccatg gcaacagagt tcccatagta gtctgcggac gggaaagccg
     2401 tctacatggc aaaggggaac agtgaatcat tttcaaaaca gaaaggatgg tgtgtgagac
     2461 actatgagaa atccgattca tgtcttgaaa agccttgaag aaaaggcaag tgtaagtaac
     2521 tacaaatatg aaagattata tcgcaaccta tacaatccag agttctatct ccttgcatat
     2581 gcgaacattg cgaaatcgca ggggagcatg acgcaggggg tagacgggca gacgctggac
     2641 aacatgagcc tgccgagaat taaccggatt attgaatcca tacgcaacag gacatatcag
     2701 ccaaagcctg caaaaagaaa gtacattcct aaaaagaacg ggaaacttcg cccgttggga
     2761 atcacttcta ctgatgataa gttagtgcag gaagtggtca gaatgattct tgaagcaatc
     2821 tatgagccga cattcagcaa caattcacac ggtttcagac caaaaagaag ctgtcacacg
     2881 gcacttacgc aagtgaagaa aaacttcaca ggtgttacat ggattgtcga gggagacatt
     2941 aaggcgtgct ttgataactt cgaccatcat gtgttggttg aattactgcg gaaaaggatt
     3001 tcagacgagg cttttatcgg tctaatctgg aagttcctga aagccggata tatggaacaa
     3061 tggcagtaca actgcaccta ctccggtgtt ccgcagggca gcggtatcag tccgatatgt
     3121 gcaaacatct acctcagcga actggacaac tatatgcagg aatacaaaga aaagtatgat
     3181 tgcgagccag aacgcagaag gacgaccaga gaatacgagc gggcgtcccg gagatacaga
     3241 aaggcacgta aagcgttaat gggcgcagaa aaatcaactc ctgaactggt aaaagaattt
     3301 aaggattcca gaaggaagaa gatgaatcag cactattaca atccgtttga ggaaggcttc
     3361 aagaaaatcc agtacaaccg ttacgccgat gattttgtaa tcggcgttat cggctccaaa
     3421 aaggacgcag aaaaaatcaa ggaagatgta aaaatctttc tccaagaaaa actgcatttg
     3481 gaaatgtccg aggaaaagac gaaagtcacc cattccagta agccggtacg ctatctgggg
     3541 tacgatttca aagtaatcca ttccaagaac atgaagcgtt gtaaaaacgg cgacatgaaa
     3601 cgggtgtggt atggaaaagt attcctgtat atgccgaaag aaaaatggat taaaaaagcg
     3661 atggaacggg gagcgataca ggtgaaacgc aacaatgaca caggcaaaga aatgtggcgg
     3721 cctatgccaa ggaaagacct gatgaaccgc agcgacgcag agattgtgtc tactttcaat
     3781 tccgaaattc gagggttata taacttctat cggatagcag aaaacgtagg cgcattgcac
     3841 aagtattact acatggtgcg gtacagcatg ttaaaaactc tggcaggaaa gcacagaacg
     3901 aatgtcagtg ttattaagaa gcggcacatg gtaaacggag tactgagaat cccatacgat
     3961 acaactaagg gacgtaaata ctgcgaattt taccatgatg gattcagaaa gcacagcgac
     4021 ggctatgaca atgtggcaga cgttatgccg agttatagga aatatgacag caggcacacg
     4081 atagtaaacc gcataaaagc cggagtatgc gagatttgcg gggaacatgc agactattta
     4141 tgtatgcacc acgtaagaac gctgaaatcg ctgaaaggca gggatatatt tgagcagaaa
     4201 atgctgaaaa tgagaagaaa atctctggct ctctgccctg actgctttga actcttacac
     4261 gaaaccaaag aatcaaggtg attcatggaa agccggatac gctgagaggt gtacgtccgg
     4321 tttgggaggc ggctccctga aacctactga tgaaagtcag caaggcgcag ggtgcctacc
     4381 tcatacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat
     4441 ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt
     4501 aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc
     4561 cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag
     4621 ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg
     4681 atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa
     4741 atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc
     4801 atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa
     4861 ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt
     4921 gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac
     4981 accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt
     5041 aatttcattc tgactatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat
     5101 gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac
     5161 attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg
     5221 gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag
     5281 atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag
     5341 gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg
     5401 ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa
     5461 gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg
     5521 tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc
     5581 gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag
     5641 caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac
     5701 gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat
     5761 gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac
     5821 tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct
     5881 gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcacttaat
     5941 gtcttctatg tgaaaagatt ttaaagagaa aatgcacatc ctacgcagat aacatgacaa
     6001 tttcatgcaa agcgaaagga gacaccatga aaatcgaata cacaaaagtg ggagactatt
     6061 atttaccgaa tctttactac ccggaggaaa ccagaccgat tggcttatgg ggaatgcttc
     6121 ggaaggaata tctgaaagaa cataaatctg gaacttatac atatcttctt ctgaccgcca
     6181 ggctggacag ttatttggca gatctgaatg aacaggcaca ggaacgcttt gaattgatcg
     6241 aaactcagat gcgaagtgcg gagggagttt ccgaggaact gaagagacag aatcctatgg
     6301 aatggctatg ccactgtaac aatatccgca accgggctgc tgagatcatc aagcaggaac
     6361 tgatttatgt ataaggggga ttctagatat gagaagagaa ctgattgaaa gttattatga
     6421 agaataccag cgggagctgg aaagatttct tgaatctgac ccggagatgt tcactctgga
     6481 agcagaaatt catcattaca tagaaatact ggaaccggtc ctcagagatg gcggacagga
     6541 gatgtggcag acactggaca ggattatttc agcgaaaaat gcaatggagg gaattgtggc
     6601 aaaagaaatg tatatccggg gatttctgga ttatgaaaga ctggtgtgtg aaaaagacag
     6661 taagatctga ataatcagaa catagactat atataacgag gcttttattg tatagacagt
     6721 aagagcctca tttaatatag ttttcataat aaaaaacagg tggtagtacg tatttgcttt
     6781 gtgttatata agaattggtc tggctgaaca atggtgaaaa tttactatga ttttacttaa
     6841 atctgattga aggatgaaaa agtgatgata tcatatagtg ggttgatagg gttacagaaa
     6901 atgtattcac agaggcagag aagaaataat gaaagtaaaa aatgcagatg aattttataa
     6961 aatattagaa agaaaggcta tatatccggt atttcagccg attgtaaatt tacagactgg
     7021 agatgtggca ggatatgaag ctcttagtcg tatcgacagg catgatacga ctcttatgat
     7081 ttcagatttg tttgtgattg ctgaacaggt aggctgtgtt tggaaattgg aaaaattgtg
     7141 cagaaataaa gcattgaagg cagcggcgaa taaaccagaa catgcaaaat tatttcttaa
     7201 tgtagatggt aatatcattc aggataaatc gttcatacag ggatttacaa accgtaaggc
     7261 tgcgaaagcc ggagtaccgt cctgtgacat tgtatttgaa ataacggaac ggtcggatat
     7321 agaaaactat cagattctgc aacagattat gaagcattat gcagatcagg gatatgaaat
     7381 agctttggat gatgttggag ctggatattc tggtctgaac cgtgtagtaa atacatcgcc
     7441 aaactactta aaagttgata tagaactggt cagagatatt caaaaacata agaaaaagga
     7501 aattatgatg gaatttctgt tgcattactg taatgagaca ggagcaattc tgattgcaga
     7561 agggatagaa acagaagcag agttggagtg tctgtgtaga ttaggtgttc attacggaca
     7621 aggatatttt cttggaagac cggacagaac gttcaaaaat gtaaggaatg aggcgatgtt
     7681 ggtattacgt agactgaata ataaatcgta atgaaaacta tatgtcagca ggtgaaaaat
     7741 gaataagtca attataacat tataaagatt tccattgatt tttacaccta ttttacacaa
     7801 gcactcacat agatttgatt tctgtcttct ataattgtat aggaaaatgt agatcttata
     7861 taataacagg agaggaaaga gaaatgaacg agaatgtgaa agtggttttt ggtctgatcg
     7921 gtggccttgc cttgttcctt tatggaatga acagcatgag tgatgcactt cagaaagccg
     7981 caggagaacg aatgaaaaaa atccttggat ttcttacaag aaatccaatt atgggagcac
     8041 ttgccggagc acttgtaaca gctgtattgc agagcagttc agcgacgaca gttatggtta
     8101 tcggttttgt cagtgcggga cttatgagtc ttccgcaggc aatttctgtt atctttggtg
     8161 ccaacatagg aaccactatg acggcacagc tgatggcatt taaaatcagc aattatattt
     8221 atccaattat ctttgttgga tttattctga actttgttag caaaaaagaa aaggttaaaa
     8281 atatcggtat ggtaattttt tcattcggac ttttatttga aggaattgaa atcatgggtg
     8341 aagtcatgaa acctttggcg ggaagtccgg tctttgttga tctgatggga aaagtttcat
     8401 ccattccggt tcttggtgtt gtactgggag cggtcatgac attggttgtg cagagtagtt
     8461 cagctacgat tgcagttctg cagaattttg catcacaggc aggaccggac ggagtaagca
     8521 gtgtaatagg gcttaccggt gcgattccga ttcttcttgg agataatata ggtacaacga
     8581 tcacagcact tcttgcatca attggacaat ctaaaaatgc aaaacgtaca gcaattgcgc
     8641 atagtatttt taatatcagt ggaagctgcg tttttatttt cctggttccg tggtttgcaa
     8701 aatttgtcca gtttatttca ccaaagggaa atgagattga tgtgatttca agacagattg
     8761 caaatgcaca tacaacgttt aatatcgtct gtacactggt ctggctgcca ctgattccgc
     8821 tgatggtaaa aattgttaca acaatcatcc gtggaaatga caaaacagag aaagcagctt
     8881 ttgaacccaa atatctggat atgaaagtga ttgaacagcc ggcggctgct atggttctgg
     8941 tatctaagga actgaaccga cttggagaac tggcagaatc tctgttaagt gatttaaaaa
     9001 cggcgattgt agcagatgga gactcaaaaa ctcatggttc gtttatagaa aatctggaga
     9061 ttgtacatca gttacaggac agtgtgacag aatatattac cagattattc gcaagcggaa
     9121 accttacaga acaacagtct gagcagacgg cagggttact gtatgtaaac aatagtattc
     9181 agaggattgc agacagatgt gaagatattg accagatatg tgaaaaagta aataacaatg
     9241 gaaaaagact gaccagtgaa gcctcctcag aaatggaaga ctgcattgat attatccagc
     9301 agcttttaaa gaaagcaatg gaggcaatcc gtaagggtga tgctgtagtg gctgaaacag
     9361 tttttaaaaa taagaaaaaa atgcataaag cagagaaaaa atacagtaag gcacatttaa
     9421 accgtgtaaa aaatgaggtt tgtgatgcgt ctatgacaag atatttttct gggatcatgt
     9481 ataattttga ccgtatggca gataattgtg tcagcattgc agaagaagcc tgtgataatg
     9541 tggcttttat aaaccttgat gaagagacgg gaaaatcaat gatggaaaga ggtgcagtat
     9601 aaaatggtaa agaaaaaaaa catccgaaaa tctgcacttc tgctgataat gataatgctg
     9661 gcaacgggaa taacagcatg tggaaccgag gaagagccat tgccggatct gtctgctatg
     9721 ggagctgtca cagctgtatc acgagaagaa gggtcaggaa ccagagcaga atttgagaat
     9781 cttttgaaac tgcctgagag tgatacagga atagttgttg attctacaga gaaagttctg
     9841 aaaaaggtgg aagaagataa aaacgcagtt ggttatgtcg catatagttc cgcaacagat
     9901 acgaatggaa aaatacttca aatcaacgga gttcttcctt ctgagaaaac aatagacaat
     9961 aacagttatc ctctttgcag ggattattat ctggcttata atggagaact gacagatgtg
    10021 gaacaggatt tcctgactta tgtaaagagt aaaggacagg atattgtgaa acaatattgt
    10081 attcaggctg acagtacaac aacattcctt tctgacaagt cagaaggaaa gattctgatt
    10141 gaaggatcca cttcaatgga accaatggta aaagcactgg cagatgacta tcagaaacaa
    10201 aatccaaatg ctgaaataga agtaaaagca acagattctt caagagggat tacggcggtg
    10261 ataagtggag agtgtgattt cgcaatgtca tccagagaat taaaggatta tgaagcagag
    10321 cttctggaaa caaaagtcat tggaaaagat gctatagcta ttgtgataaa tgaagaaaat
    10381 ccactggaaa atcttacaat aaaacaactt acaggtctgt acaatggaac atataaaaac
    10441 tgggatgatc tttcctgaga aaaacgaata taatatctta caagcactga ctataacggt
    10501 cggtgcttct tttttggatt ttttcaaaat tatctgacat tcttcaaatt cccacgtgtg
    10561 attagtggaa aaataatttt ttctgcaaaa gtaccgtcca aatgccctct gaaacttgct
    10621 attagtgagg gaatatttca tttatgcgaa aaaaacttgc aaaagggggt tggtaaatac
    10681 cgcttcccag gctttatagg tagaggaaca attattttaa aaattttacc ccaaagatgt
    10741 ccgcttttgc ctgtctcagt acagagtatg tgaagggact aacaaaagta aaagaaaaaa
    10801 tgataaaaat gcaaaaaaat atcacgaatc ttttgcatct tgcgaagtca tttgactcta
    10861 gtaatcagag gacgttttgg aatgttcctt cgatgaacct tgacaaccga taagtgcacc
    10921 ctagaagcct aacaggagtt atgccatgac ctttcttttt ctgagagcga gcgaaattat
    10981 aatgacactc ccgatgatgg gcggctgacc gaagccttat ggtcagtgac ctgctgatgc
    11041 aggcagaggc agtctgagac ggctgcaggt gacagggaaa cgcacgccag accgccggac
    11101 gaatgaaact gatgacaatg tttacagggc aaaatgcccg gaaaggagca gtttatgaca
    11161 gaactgaaaa tgaaagaaat gcagttgatt aactggtttg gatgcccgaa tctgatttat
    11221 acaagggaga gaatccatct ggttgccatg cttacaacag atgaagaaat gaaaaagaat
    11281 ctttacaggg tatgcagatt ccttgcaaga gaagaaatcg catatcggta tccgatcatg
    11341 tatgaaatga tccggaaaat attttcaccg gatgatacca atccaccgga agcatgtgca
    11401 atgtttatgc cggacgatga aacagatgat gaagaatatg caatggcagc gtagctgcgg
    11461 aaaggacagt aacgattatg aataaagtgg ttttaatggg aagactgaca aagaatccgg
    11521 agatcaaata tgccggaaaa gataatgata tggcagtagc aagatataca ctggcggtaa
    11581 acagaagata taaacgtgac ggtgaacagg aagcggattt tatttcctgt gtcacattcg
    11641 gaaagagtgc agagtttgca cagaaatatc ttcacaaggg aatgaggatt gtgattggcg
    11701 gtcgtatcag cactggcaat tataaggaca aggacgggaa aacaatttat actacagatg
    11761 tgatcgtgga agaacatgaa tttgcccaga acaaggataa tggtgttgga gctgattcat
    11821 cggaaacacc gaaaacagat aaggacggat ttatggaagc accggaaggt gaggttccat
    11881 tcgactaaac cgaagtaaat ggttggagag gttgcccttt gtcggggcat cctcttttcc
    11941 attttacaga agacatcaaa aattacaggt attcaaccta acgaaatcgt gatataatca
    12001 aaatatgttc agatgaaaaa tatggaggtt gcttatgggc gtaagttata acagattatg
    12061 gaaaatgctc atagataaaa atatgaagcg gatagagatg cagtacctca ccgggatcag
    12121 tggaaatatt cttgccagga tgggaaaaaa ccagtatgta tcaatggaga cgattgagaa
    12181 gatctgcaag aagctggact gcaccgttga tgagatgctg gaatttacag atgatgagta
    12241 acaagagcag acatgtcaaa ttattaaaac tggaagaatg cttcagaagt cgaaaaaaga
    12301 aattgaaaaa agataacacg ccggatacgg cataaatctc ttagcaacgc taagagcgca
    12361 cttctataca ggcaattcca tctgcctgta tcgtgcgacc tgcggtgctg ttcccgattt
    12421 caagaaatct caaaccggga aatacagcga aatcagagat ttcgtacaag gggaagatcc
    12481 ccttgcggca cagatgccta tccctgataa agtttgtgac tgaatatttc agatatagac
    12541 atataagggg aaagaaagtg tgaaatcaaa atttgtggag agattttatg attagaaaag
    12601 caatactaaa agatgttaat tcgattgaag atacttacaa tgagcatttc cagtatgaac
    12661 tgaatcatac agcctttact gtatttaaga aaggggttta tccaacgaaa gatgatgcag
    12721 aacgggctat ttatgctggt gctttgtttg tatatgaaga aaacggaaca attgttggaa
    12781 gtatcattat tgataaagtt caaccaatag aatatgcaac tattccttgg aaagaaaaat
    12841 tatctgaaga tgaggtaatg gtaattcacc ttttaatggt acgtccgagt atgtctggta
    12901 aaggaattgc atcatcacta ataaaattcg ctacagaatt agcacaaaaa aatagttgca
    12961 gggcactacg tttggataca agaagtcaaa atattcctgc gctatctttg tatcaaaaaa
    13021 atggatttga aataattgct agtgcttcta aaaaagttgg agatgtaatt gcacacaaaa
    13081 accatctttt tctcgaaaag atattgtagt tcgtaaattc cagtttgaaa aattgagaaa
    13141 atcggagttt gaggagcatc accgttatga ttagagaatt acggaaagtg gatataaata
    13201 aagttgcaga aatatggtta gatacaaata taaagacaca ttattttatc tctgctcaat
    13261 attggaaaag taattttgag ttagtaaaag aacttctatt acaggcaact gtctatgttt
    13321 acgaggataa acaggaaata cagggtttta tagggttaag taacgaatac attgaaggta
    13381 tttttgtttc tgccgaaatg caatctcaag gtataggtaa aattttgcta aactatgtaa
    13441 agggaaaaag aaacaaatta atcttaaatg tatatcaaaa aaatacacgg gcaatatcct
    13501 tttatcaaag agaaggattt gagattcagt atagcggttt ggatgaagct actggagaaa
    13561 aagactatgt aatggcatgg cagcagaaat agaaattcaa gttttagtgt ttggaaaatt
    13621 atgacttagt gtgcaagttg ggagaaaata tggtaacgga aaaagagaaa aaagcatgtc
    13681 gatttattgc cgaatggcta taagattgtg agacagtctc tggtagatga tctggagggc
    13741 gaaaaaaagg tatgttggga gacagaaatt ttgcaggatg atgatagtgg atttacttat
    13801 gaaatcagca ggtgtctgta tttcgatacc tgtaaggaac atggctatgc agagttctgc
    13861 aaagtatttt gcaaccatga ctggtatgct tatggagtat taaagcatca ttcgagattt
    13921 atcagaaaat cgacgattgc cgaagatgga actgtatgtc atgatacaat cgagaaagta
    13981 acagagaaac attttttata aaaccgaaaa attgattatg ggaggaaatt gaaaatggcg
    14041 aaagaagaga gatttgaaaa aatatattct cagggagcat taactactac tgagatatgg
    14101 gtggataagg aaactggtgt gaattattta tatcatgcat gtgggtattc aggtggactg
    14161 actccattgt tggacagaga tgggaaacca gttatttctc aggctataaa tagatgacct
    14221 tagaaacagc aactgttatg gtaagtaaca gcatcgtcac aatatgtata tggtcgattg
    14281 gtgtgtgcgg taatatacta tattcagaaa aacaattcag gagattttta tgagaagatc
    14341 aggttggaca aaatggttga tcgtgattcc ggcaatgcta ggcattgtgt tggtgaccta
    14401 tgtgcggtat acaacggata tctggggatt tggtgcattt atctgccagc tgattgcaat
    14461 tttggaattg gcttatggtc tgcgaattgc gatgctggca cagaatagaa agaagtccta
    14521 tcggttaaca ccggaagaac gacatgagta tgcacggtat ttatatgaga agcaatatca
    14581 ccgttaccct gctgtagcga atcagatgct tttggttatg gcacggatga gtgtactcct
    14641 taacaattat gaacgggctg cacaggagct ggcagatatt cgtatagata aattcaatcc
    14701 agcacagttg aagttgtatt attacctgaa agtggttaca gcaatggcag caggagatgc
    14761 gacagggatt caagagagcc agatgtgtta cgcagggatt ccggatacta aatgcacata
    14821 tccgtcaaaa gcagagcttg ctatgtggat agagcagaga gcggcagcgc agatggcaga
    14881 ggcactgaag aaggcagtgc cggataaaaa agagcatccg atacggatca gtatcattac
    14941 tttggttctt gcgtatagta ccttcttcta tggattgtgg tatggtataa aacgtgatat
    15001 gggatatgaa gtgagatatc gtttcgcaga aatatcgatt actgttatta caatcagtct
    15061 ggcggttctt ttgatctggg ggatcgtgag tttatacaga cacaattgcc aggcattatc
    15121 catacaattc ggtaaaaaca aagtggcatt gataattgtc tatggagctg ctgcgatatt
    15181 tgcacttttg attattggaa tgagtggtct gagtgtagtg ttcggaatgg atggaacaga
    15241 aacggtgact ggttgtgaca gcaaatatac gtatataact gtgcaggaag attatggtgg
    15301 tgaacggaga tacagaacca ataatccatt tgttatgcag agtatggatg gtctgtggtg
    15361 gtcttcggat acaatagatg aagaagataa tcaagaagag gatactacag aattaccgga
    15421 tttggataca gagaattcaa cagattcaga ggatattcag agtggaaata ccatagaaga
    15481 ttacgagaca gaagggcagc atgatggtct gacaattcaa aatgcaatgc tggcagttta
    15541 taattacctt caggagcaga atacacttca aaatatggcg ttcagttaca cggcgaatgc
    15601 aaagggagaa gtctatgctg tcgtcagcga aacgcaggaa acaaagaacg gtaatacggt
    15661 aaatgtgaga tactgtctgt acgataatgg ttccaagaca gatgcagatg gtaattcctg
    15721 cgaagaactg gtgttagaaa aagtatatcc ggatggcaat tacgagaccg aattggtaga
    15781 tttttatctg gtaaatgcag atacgatgca ggtgacagac gaacagaaga atacgtggta
    15841 aaataaattc tggggaagta ggtgcattgt atgacaatga gagaaaaaat aaaactatta
    15901 tcgtaaatgt ttcaagcgga ctgggttctt ttggaagtat gcaaaaggac tccaagacat
    15961 aagaggtgaa ctgcgctgat ccaggtgcca cggcaacaga tttgaatgtc gacggaggaa
    16021 agcaaacagt acaggcgcaa tcgtaaaact tgcaatgatt gataaaaccg gactaacagt
    16081 aacttttatt agtcgatttg gaaatatgcc ttggtaatca acaattccgg ttttactgac
    16141 taagaaaatg aggaggattc ataatgagtg agaaaacaga gattactttt atgcaaacaa
    16201 gactaatccg gcttgcttca gaagagtggc atttacctgt tgaacagatt atccacttgt
    16261 ttaaagaagc cgatgttctt ggatatatag aaaaatgcta tgggattttt cattgtgaag
    16321 gcgatgaagc tgtgcttgaa gatattactg aatttttgca aagaaagggg atagagatta
    16381 gtgcttgagt taaaagatgg atttgttctc tatcatggaa gttattgtga agtaaaagag
    16441 ccagatcttg ccaaatgtgc aaagagaaaa gattttggac agggatttta tttaaccaca
    16501 tcaaaggaac aggcagaaag ttttctgaga acctcaatag ccaaagccat cgcaaccgga
    16561 acaattgaag aggggcagaa gtttggatat atttcaactt ttgaattcaa actttcagaa
    16621 aatcttgaaa cgcatatttt tgaaaatgca gatgtggact ggttacattg tatcgccgct
    16681 caccgaaaga aaaaaatgtt tattgaggtt gaacgtgaaa tggcaaaata tgatatcatt
    16741 gcaggaaaga ttgcggatga tgcaacaaac gctactctta ccgcttatct tgcgggtgct
    16801 tttgggactg caggtgataa agtagcagac gatttctgta tcagacagtt gctgccgaat
    16861 aaattgaaag atcaatactg ctttaaaact gaggcagcta ttgagtgtct gaaatttgtg
    16921 aaaggtgaga agatatggct gaagtaacaa ttccaaagga aaaaatgaat tatacaatcg
    16981 accttctgat cactatggtg acagatgaaa tcgcagaaga gacaggcaag gacagaaacg
    17041 aggtattgac agattttttg tgttcaaaga caggaaaagc actgtatgat gagaagacaa
    17101 agctgtggtg cactggtccg gcatatatag ctgaactgta tatggaagaa ttaaagaaat
    17161 cctgatatgg gatgtgagtt taaatttata cgacaatggt gacaaagact ttcattccaa
    17221 ggagcaacaa ttaaatattt caaaaacgct gaggtcagca tttcctaatc acaggacaac
    17281 gacctcagtt ttttataccc aggaatcggc attcatgaat aagccggttc tttttgttta
    17341 cagaaaaaag aaggagtaca agaaaatgag cagagcaaaa aatccaaagt atgaatccat
    17401 gtcattggag gaactgaagg ccaacatgga aaagagcaga caggaactgg aggcagcaat
    17461 tcaccacaaa aacattctgg agcagcggaa gaagctggta gaacgcaggg aacgttccca
    17521 ccggctgatt gtcaaagggg cagagtttga gaaagtattt ccgctgtcca gagatctgga
    17581 acaggaagac gtccaggaca tgatgaatca actgcagaac agcagttaca acaagagtat
    17641 tgtgcggcag gctcatatag ccgccttaca caaagatcaa cagcagattg cagaagcagt
    17701 aaaaaagaca gagaagggag atgacagtta atggcactgt atcatttttc agtaaggaat
    17761 gtatccagag ggaaagggca gatggtggtc gcttccgcag cctatatttc cgggcagagg
    17821 atttttgaca gttattacaa caagatacac gattatacga gcaagtcagg tgtgatattt
    17881 acagaaatcc tgacaccgga gtatgtgcca gagcgtctga ctgacaggga aacactctgg
    17941 aacgaggtgg agcatgtgga acggaacaac aaggcacagc ttgcctattc gtttgacatt
    18001 gcactgcaga atgaattgac acttgaggaa aatatcagac tggcaaaaga gttctgccag
    18061 gagcagtttg tggcacgtgg catgatcgtg gatctggcag tccatgaggg aaaagcggaa
    18121 aatgaagaag aaccggataa tccccatttc catgtgcttg tcccaatccg tccttttaca
    18181 gaggaaggaa tctggggcaa taagcagaga agggaatata ttcttgatga ggatggaaac
    18241 cgggtgaagg atgccaaagg aaaagatatg ttcaatgctg tttctactac cggatggaat
    18301 gatccggagc ttttaaaaga atggcggcgg gcatggacag agaaagtaaa tgggaaattc
    18361 agggagtgcc acatggcatc cagaatcgac caccgttcct acaaagaaca ggggattgac
    18421 ctgatcccga ccatccatga gggatatgaa gtccgggcaa tggagaaaaa gggaatcaaa
    18481 acggtgatcg gagaattgaa ccgggcaatc cggcagttta accagatgtt tatttctctg
    18541 aaagaatcca ttcagtggat gaagacagct tatgaggaaa tgaaagcaga gctggatcgc
    18601 aggcagaacc cgacactgct ggaaagcctg caggattatt atgataaaaa gacacagggc
    18661 agaccaccac ttcccaattt ttatgcggag atgaaacaga gaggtaagaa tctctccaat
    18721 ttacaggagt ttgcaaaatc catcagttat cttcagacac atcagataga aacgatggat
    18781 gacttgcagg aacggattga ggaactgaac ggtgttgttt ctgtcagtaa aaaagaaatt
    18841 tctgaaaagc ggaaacagtt aaaggaactt gagaatctgg aaaagatggc agaggtaata
    18901 aaaacaaatc agccattgat tgatgagtat aaccatttct attttcagaa aagacgggag
    18961 agatattatc agcagcataa aaaagaaatc aattattacc gcaaatgtga acgggaactg
    19021 aaacaacatc tggataaaaa tggaaaagtg ccaactgcca gatggaaacg ggagaaagaa
    19081 gaacttcagg cggggatcga agagctgaaa gcagataatc agccgtacca ggaggaacta
    19141 acttttgtga aaaaggtaca gagctgtgcg gatattgcca gacgtgaccg ggaaatggca
    19201 gaagcagata cttccgggcg ttcagaagaa aaaagggaga agcaggtgaa gttcccgaca
    19261 ttttacacag ttcaggcgaa agagattctt gaagaaaacg ggaaagcaga gcaacagccg
    19321 ttgaaccagt cggaacagaa tccagaaaag aagacctctc ttctgagaaa actggatgaa
    19381 aaaaagaaag agtgtgcaga gcgtgatgca aaacaacagg cagtgaagaa aaagcgaaac
    19441 tatgacatga gtttataaca gtctgtcagc aggacagacg ggaaaggatg agcaaaatgc
    19501 agtatacaga agaacaaatt ataagagcaa atcagacaga tcttgtttct tttctgagcg
    19561 cacagggaga acaactaggg aaaagcggaa aggaataccg ttggaaaaag catgacagtg
    19621 taacggtatc cggaaaccgg tggtacaggc acagtcaggg gaggggtggc tatccggtgg
    19681 attttgtgat ggaattttac aatgccacat ttccggaagc tgtcaagatg ctaaccgggg
    19741 aggaaggcga aggcaggaac agtacctgcc ccgccccgtc ccctgacttc cgattaccag
    19801 agaaagagga aaataatgat agaatcatca gatatctgac agaaaacaga gggattgaaa
    19861 agaatatggt ggaggaatgg atcggcagtg gggatattta tgaagaaaag aagcaccata
    19921 atgtggtgtt tgttggcaga gatgcagacg gtattccaag atatgcccat tgcaggggaa
    19981 ctggtgaaac aaaataccgg ggagatgtgg ctgaatctga taaatcttac ggattctgtc
    20041 acagagggac ggataatcag ttgtttgtgt ttgaagcagc cattgatctg ctttctttta
    20101 tccaattatt tcccaaagac tggaaaaagc ggagctacct gagtctgggt ggcatttcca
    20161 gtgcggcact gatggctttt ctttctgaac gccctcagat tacttcggtt ttcctgtgcc
    20221 tggataatga ccatgcaggg aatgaagcca gtgagaaatt ggcaatagaa atcccggacg
    20281 gatacagtgt catccgtctg aaaccgtccc gaaaggactg gaatgaaatc ctctgtgata
    20341 aaaatgcaga caggaagaaa tcaatcattg aaacagttac aatgaaagta ccggagaagg
    20401 aagaactggt gccgatgttg tgttatgaag acattgaaca gaccagtgta gaatggctgt
    20461 ggtttcccta tctgccattt ggaaaactga cgattattca gggcaatcca ggagagggaa
    20521 aaacttattt tgccatgatg ctgaccgcag cctgcacgaa ccgtaagact ttcccgaaca
    20581 tggaagagat tgaacctttt aatgtgatct atcagactgc agaagacggt atgggagata
    20641 cgattaagcc gaggctggtg gaagcaggag cagacctgag tcgggtgatg gtcattgatg
    20701 atacagagga agcattgacg ttatccgatg accgtattga aaaagcaatc cgtcagaatc
    20761 aggtcagact tttaatcatt gatccggtac aggcttttat cggtgccgat gtggatatga
    20821 accgggcaaa tgaagtccgt ccggtattcc ggaaactggg aatgatcgct gaaaagaccg
    20881 gttgtgcgat tgtgctgatc ggacatctga ataaatcttc agggacacag agtacttacc
    20941 ggggacttgg ttctattgat atcatggcgg cggtgagaag cctgcttttt atcgggaaag
    21001 taaagaaaga cccgacaaca agagtactga tccatgagaa aagttctctt gcacctccag
    21061 gtgagacgat ggcatttaaa cttggagatg aagaaggatt ccgctgggtg ggagcttatg
    21121 agatttccgc agatgatctt ctggacggaa aggaaggaaa gccgacagaa acaaaactgc
    21181 aacggggaac gaaactgatc tatgaactgc ttgcagacgg aaatgcagtc acaatccggg
    21241 aactggatga aaaggcaaaa gcacagggaa tctcccagag aaccatgcga gaagccagaa
    21301 gccggatgaa agaagaactg gattatcgga tgaatgaaaa gcaggaaaat accattcgat
    21361 taaaaaaaca agggagaatg ggtgacggca gaattttgga gtgaacggca ggctttatag
    21421 ataccctatc tgccgtctgc cgtctgccgg tgcaaggcaa tcaaacggaa atagcaggta
    21481 tccatacaga ataaggattg ctgtgaatct ggaacaaaaa tgaattgtaa tatggcggat
    21541 ataatggtat gattatccgg taacagcaat gttacgggga agcacctgtg acagggtgtg
    21601 gctgcctcct gatatttaca gaccggagcc tttcggggtt ctgagatgaa agaaaggagg
    21661 tgcgtgatat gacagcttat gagattatct caatattctt aggcgtactc agtttactga
    21721 tcgcttttgg tagcctgtta attgcgatta ttgcctttct tgatcggaag aacaagagga
    21781 aataaaaatg cctatcctgt ctgccaacag gataggcggt ggtcgtaaga ccatttagtc
    21841 aactcaggcg gcatccactt tggagtgggt gcttttccta taacaaatgt accacacatt
    21901 cctcttgttt tcaatgggaa aattaaaata tgcagttcat ctgacaggga tttatccctg
    21961 tctttattat atccccaatt tctgaaatgt taatacaaaa attttaatat atgtaaccaa
    22021 cagtctgggc agttaatctg taaaaatgga ttagctgctc tttttttgca aaaaatggag
    22081 gttttacaat ggcagtgaag aagaaaatta tcaacatcag agtatatttt gacggtgatc
    22141 aggacgcaaa agaagtgttt gcagaggtga tcgcacagaa aatacgtgac acgaaaacaa
    22201 aagataaact tgcaataatg aaagataagc agtataatga ttatgagttc agtaatctga
    22261 aatctgcgta ggatgtgcaa agtaacggag gtatcagatg aacaacagat atatgagtaa
    22321 caatatagaa agaaatgcaa cctggagagt tggtgtatac tgccgacttt ccaaagatga
    22381 cgaattgacc ggagagagtg ccagcatctc caaccagaga gacatactgg tgaattactg
    22441 tcagtcacag ggctggcagg tggtggatgt gtttcaggac gatggttata caggattgaa
    22501 cacagaccga cctgatttgc agagacttct caaagcctgt gaaaaaggac tggtgaatct
    22561 tgtaattacc aaagaccaga gccgactggg acgaaaccat gtacagacag gatttctgat
    22621 ggaggagttt ttcccaaagc atggagtaag gtatatcgca ttatatgata atgtggatac
    22681 ctttgccgga gacaatgaga ttgccccatt taagaacgtg ctgaacgaga tgtattccag
    22741 agatatttcc aagaaagtcc atgcgtccta tcacctgcag gcaaccaagg gaaagtttac
    22801 aggtgttgtt ccaccactgg gatatcagaa agacccggag gaaaagggac atctcctgat
    22861 tgacgacgag acagcaccga ttgtccggaa aattttccag tgggcggtgg acggtcatgg
    22921 gattaacttt atatccagag aactggaacg ccagcagatt ccctgcccgt catggtggca
    22981 ccggaaacgg ggactccgca cctattacac aaaatgggaa aagaaagacc cggaaaatgg
    23041 gaaatatgta tgggatgaat cctatctgaa atccctgctg atgaatcccg tttactgtgg
    23101 tgacatggct tcccagaaaa ggtactaccg tttcaagatt ggcaatcagg gagataaagc
    23161 tccggaggac tggatcacag tcagggatac ccacgaagca atcattccgc aggatgtatt
    23221 tgatctggta caggcaaaga tgaaaggcag gaaacgccag acagaacagg gagaatacag
    23281 catgtttgcc ggattactcc gctgtgcaga gtgtggcggt gcgttgacgt tgaagaagac
    23341 gcacaccaaa gaccagcatg aagtttatac ctgttccact tatatccata aggggaaagc
    23401 acattgtacc cagcaccggg tggacgctga tgacctgtat gatgcagtcc ttaccagaat
    23461 ccaggaatgt gcaaaagcag ttaccggaga cggaacagaa ctggaaaacc gggtaaagga
    23521 gttatgcgag gaagatacac agggacacag ggaaagtctg gagaagctgg tatcaaagca
    23581 gaaagaccgc ctggaaacgt tagaccggct gattgcaaaa ctgtatgatg accttatcaa
    23641 tgacaagatt acagaatctg tttttgataa gatgctggag aagacacaga aggagcagac
    23701 agatatcaaa aaggaacttt tccagaatga gagtgtgctg aatacggaag aaaaactgga
    23761 tgcacagtcc cagcagtgga ttgacgatat tagcgagtat gcggacatca aagaactgga
    23821 tgccaatctt ctgaaccggc tgatcagtaa aatcgttatc tcagaaccgc aggagaaaga
    23881 cggaacagaa aattactgcc gtactcctga aacggtcaca atggaaattc atttcaactt
    23941 aaaaccaata ccggagcttg gaaccatcga aagaggttct ggctcccata aataagaaaa
    24001 atctatcaaa atttaatcta ttttaatctt ttcaaggctc atttcgagca aatattttct
    24061 cataaaagac ataaaacagc ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct
    24121 ggttcccctg ctgtccgggc tgctgggctg atcctggcct ttgtccctgt ccaaaccctt
    24181 aactaatcgc ctccgcgtcc tcccggccag accgggaggg cgcgggaaag gaggtgctac
    24241 ccgtgattag tgaaatcatt gaagaatgga taaaaggcat cctcatggac ggtatcacgg
    24301 gcaacctgtc cgggctgttc gatacggtaa atgccaaagt cggagaaatc gccgccgacg
    24361 tgggacgaac cccgcaggca tggaacagcg gtattttcaa tatgctgcac agcctgtcgg
    24421 aaaccgtgat cgtccccata gccggggcca tactcgccct tgtcatgtgc tatgagctta
    24481 tccagatgat aacggaaaag aacaatatgc acgactttga cagctccatg ttcttccgct
    24541 ggatattcaa atccgcgttt gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg
    24601 gcgtattcga cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga
    24661 catccattga ttttaacacc ctgataccag accttgaaag ccaattggag gctatgagcg
    24721 tgggcgctct tttggggctg tggttccaaa cccttgttgt gggcctcacc atgaatatcc
    24781 tttccatctg cattttcctt gtgacctatg gccgcatggt ggaaatctat gttgtcacgt
    24841 cgctggggcc tatccccctt gccacaatgg gcaacgcgga atggcgcggc atgggccaag
    24901 ggtatctgaa atccctgctg gcgctgggct tccaagcctt tttaatcatg gttgtggtgg
    24961 gtatctacgc ggtgctgata cagaatatcg gttccgcttc cgacatatcc ggcgcaatct
    25021 ggggctgtat gggctatacg gtgctgctgt gcttctgcct gttcaagacc ggcagtatat
    25081 caaaggccgt attcggggcg cattgatccc aaaatccaag catacacagg aggaacttgt
    25141 ttatttcaac gtacaacatc atttacgctg acccgccgtg gcggtatgac tgcaaaaacg
    25201 tacagggcgc agcggaaaat cactattcca ccatgagcat tgacgagctg tgcgccctgc
    25261 cggtggaacg gctggcgtcc aaagactgcc tgctgttttt gtgggccaca ttccccatgc
    25321 tgccggaagc tctgcggtta atcaaagcgt ggggcttttc attcaagacc gtggcttttg
    25381 tatggctgaa acagaaccgt aaaagcctta catggtttta cgggctgggc cgctggacaa
    25441 ggggaaacgc ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct
    25501 ctgtccatca gtttattatc agcccgattg aacaacacag caaaaagccg gatgtgaccc
    25561 gtgaaaaaat cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt gccagacaga
    25621 aaacccccgg ctgggacgtg tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa
    25681 aggaggtgcc aaatggctta tgtaaccgtc cccaaggact taacccatgt gaaaagcaag
    25741 gttgtgtttg ggcttaccaa acggcaactg atctgttttt ccggggcgct ggtgatcggc
    25801 gtccccttat attttctcac ccgtgactat cttaccaaca gcgcggcggc cctgctcatg
    25861 gtatttgcca tgctgccggg ctttttgctt gccatgtacg agcgtcacgg ccagcccctt
    25921 gaagtggtga ttatgcagat catccagtgc tgcttcatcc agccaaagga gcgtccctat
    25981 cagaccgaca acacctacac cgcccttgtg cggcaatacc agatggaaca ggaggtaaag
    26041 gccattgtcc aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga cccgcgccca
    26101 gcgaaaagaa attgacgcgg taatccgtaa atataagggc gacggcaggc cccacacggc
    26161 gcagcagagc atcccctacg aggtcatgta cccggacggc gtgtgccggg tatcgcccgg
    26221 cgtcttttcc aagtgcattg agtttgcgga catcagctat cagcttgccc agccggacac
    26281 gcagacggct atctttgaaa aactgtgcga cctgtataac tatgtggacg cctccattca
    26341 tatccagttt tctttcctca atcggaaagt tgacccggtg cagtacgcca agagctttga
    26401 gatcgcgccg cagggggacg acttcgacga catccgggcc gagtacaccg gcattctgca
    26461 aaagcagctt gcaaacggca acaacggcat ggtaaagacc aaatacctga cattcaccat
    26521 cgaggccgaa agcgtgaaag cggcgcgagc cagattgaag cgcatcggct ttgacctgtt
    26581 aggctatttc aagagcatgg gggccgtagc ccatgtgatg gacggctggg aacggctgaa
    26641 cctgctgcat ggcgtgtacc acccggacgg cgagatattc aattttgact ggaagtggct
    26701 ggccccgtcc ggcctctcca ccaaggattt tattgccccg tcctccctgt gcttcggcaa
    26761 tgcaaagaca ttcggcatgg gcggcaaata cggcgcggta agtttcctgc aaatcctgtc
    26821 cccggagctg tcggacgata tgctggccga cttcctcaat acggaaagcg gtgttttagt
    26881 caatctccat gtgcaggcca ttgaacagac caaggcgatc aagaccatca agcgcaaaat
    26941 taccgacctt gacgctatga agattgcgga gcagaaaaag gccgtccgca gcggttacga
    27001 tatggacatt cttcccagcg accttgccac ctacggcgag gacgccaaga agctgctgac
    27061 aaagttgcag acccgcaatg aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc
    27121 cgacaccaag caaaagctga ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca
    27181 taactgtccc cttgtccggc tggactacca gcaggaacag ggccttgcaa gcagcctgcc
    27241 gctgggcgtc aatcagatca agatacagcg cagccttacg acttccagcg tggccgtgtt
    27301 cgtccccttt gtgacgcagg agctttttca gggcggcgcg gccatgtatt acggcatcaa
    27361 cgccaagtcc cgcaacatga ttatgctgga caggaagcag gcccgttgcc ccaatgcctt
    27421 gaagctcggc acccccggca gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt
    27481 gttcttaacg acgcctgacg atatttttat ttcagacccg gaggccgaat attaccccct
    27541 tgtcaaacgg ctccacggac aggtaatccg gctgtcgcct accagcaagg actttgtgaa
    27601 cccgctggac atcaacctga attattcgga ggacgataac ccgctggccc tgaaatccga
    27661 ctttgtgctg tcgttctgtg agcttgtcat gggcggcaag aacgggctgg aagccattga
    27721 aaagaccgtg atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg
    27781 cccggagaat atgccgatcc tgtccgacct ccacaaagcg ttgttagatc agcatgtccc
    27841 ggaggctgac cgggtggcgc aggcccttga cctgtatgtg tccggctccc tgaacgtatt
    27901 taaccaccgt accaacgtgg acatcggcaa ccggcttgtg tcctttgaca ttaaggagct
    27961 gggcaagcag ctcaaaaagc tggggatgct cattgtccaa gaccagatat gggggcgcgt
    28021 cacggccaac cgaagccaag gaaaggccac atggtatttt gccgacgagt tccatttgct
    28081 tttgaaagag gaacagacgg cggcctattc tgcggagatt tggaagcggt ttaggaaatg
    28141 gggcggcatt ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct ccccggaaat
    28201 tgaaaatatc cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt ccggggatag
    28261 aaaaatcctt gcggaacgct tgaatttgtc caccgaacag cagaagtaca tcgacaattc
    28321 cgaacccggc gagggccttt tgatctttga aaacgtggtg ctgcccttta ccaaccccat
    28381 cccgcacaac acccagcttt acaagattat gacgacccgc ttaaacgagg tggcgggagt
    28441 atgacccggc gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga
    28501 aagtgacgca ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc accggcgagg
    28561 tggagaacat ttccagccgg gaggccgaac aggatttgtc cgcgccgggg ccgggcggca
    28621 ccgccgataa gctgctggaa cgggccgacg ccatccatga cggccataag gccaagaaag
    28681 ccgcgaagca ggccggggct gtggccgccg agggggacgc tgccctgcac cggccatccg
    28741 cccggctcca actgtcggag gaagaacggg ccgacccgga tttacagccc tatatccgaa
    28801 aatcggacaa acgggccgat aagctggata aggcccgcgc cgccatcccc aagaagcgcg
    28861 tcccggtcaa ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc gtcctgcact
    28921 ttgacaaaca ggacaagggg cctccctccc tgaaacccaa tccggcaggc cgtcctgttt
    28981 ccgaggtttt tctattcggc cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg
    29041 tggagggcgg ccataagggg gaggaacttg ccgagcggca ggccggaaaa gccatccgca
    29101 gcaccatccg gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc
    29161 tattgtctgc caacgcggag tatttctacc agaaatcttt gcgggataac ccggagatcg
    29221 cacaggcggc cagcaacccc atttcccggt tctggcagaa acagcagatc aagcgccagt
    29281 acgccaaggc cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca
    29341 agacagccgg ggcctccacg aaagcggcca aggccgcagc caaagaaagt gaacgggcgg
    29401 cctcctttgt ggcccgccac tggaaagggg cgctggccgt ccttgtgttg gggcttttga
    29461 tgctgcttat catcggcggc ttacagtcct gcaccgccat gttcggcagc gccggaagcg
    29521 gcatcacggc gtcctcttac ttttccgagg atagcgatat gttggccgcc gaggacgcct
    29581 acgcggagct ggaagccgag ctgcaagact atctggacaa ctacgaggcc acccatgact
    29641 atgacgaata ccattttgac cttgacgaga tcagccatga cccctatgtg ctgatttcca
    29701 ttctgtcggc gctccatgac gggatattta ccatcgggga ggtacagggt gatttgtcca
    29761 tgctctttga caagcaatat atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca
    29821 ccgaaaccga tacatggacg gacgcggagg gcaacaccca tacagacacc taccaagtgc
    29881 cgtatgacta ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc catgtgccag
    29941 tctatatcat ggacgaggaa acgctaagtt tctattccct gtatatgtcc acgctaggaa
    30001 accggcctga tctgttttcc ggcagccagt accccaacgc ctccaccctg acgccgccga
    30061 cctactacga cgtacccccg gaggctttgg aggatgccac ctttgccgcc atgctcaaag
    30121 aggcggaaaa atatatcggt tatccctatg tgtggggcgg cagctctccc agcaccagct
    30181 ttgattgcag cggcttcatt tcgtgggtaa tcaatcatag tggatggaac gtgggacggc
    30241 tgggggcgca gggcctatat aacatctgca cccctgtttc cagttcgcag gccaagcccg
    30301 gcgaccttgt atttttcaag ggaacctacg acacccccgg cgtgtcccac gtcggcctgt
    30361 atgtgggcaa caatgtgatg ctgcattgcg ggaacccgat ttcctacacg aaccttactt
    30421 caaactactg gcaatcccat ttttacagtt acgggcgttt accttaacgc cagaaaggag
    30481 ctttcaatta tggcaaccaa acttgaccgc atcgaaaagg acatccagaa aaccaagtct
    30541 aagatcgcgg agtttcagaa acagcttcgg gagctggaaa cgcagaaaac cgaacaggag
    30601 aatttgcaga tcatccagct tgtgcgcggc atgaacatga agccggagga atttgccgcg
    30661 ttcttacgca gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca tgaacaggag
    30721 gacaatgccc atgaagaata agaccagacg gatttcccgt attctccccg cacttctggc
    30781 cgtgatgctc tgcgtcacgg cctttcccgt taccgccctt gcaggcggca atgacccggc
    30841 accggccccg ctgccggagg ctacaaccga acccgctacg ggcggtttgg agcctgaaac
    30901 agacgaaacc ggcaccgcca cggacgggac agaaaccgag gccgaaaaag ataccggccc
    30961 tctgaccggc aaggacatca ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat
    31021 tgccgccacg gacgacggca tccagatcac caccggcacc gacaaaaaag aacctgaaca
    31081 gaccggcacc gtcaccacca acggcggcag gctgaatgtc cgcaccggcg cggggcttga
    31141 caagaccgcg ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg
    31201 cgactggatt aaaattctct tgccggagcg catcggctat gtccattccg actatatgac
    31261 cgtcagcgaa aaggaagtag cggctaccgg cgagggcggt ttttccctct ccattgaccc
    31321 ggaggaaatt gcttctctgc tggaactgtt caatggcggc ggggccagcg ccgccctgtc
    31381 cccggacggg aacctgtcac tgattgacga catcggcagc gccgcgaaaa atggcaagca
    31441 gtttatcacc gtggaaacca aaaacggcaa tgtgttttat ctgattattg accgggacga
    31501 cgagggaaaa gaaactgtcc atttcttaaa tcaggtggat gaagccgatc tgctgacgct
    31561 gatggaggac ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa
    31621 ggccggggct gtgaacacga attgcccggt atgtaaaacc aacctgaccg agtgcagcgg
    31681 cccggagccg caggaacccc agccggagga acccgaagcc ccggaggaag aacccaaggg
    31741 cggcgcgggc ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct
    31801 ttactatttc aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg atttgggcga
    31861 gtatgatttt ggggaggacg aggatttgga ggacgaggaa cccgaaaccg agtttgaccc
    31921 ggaagctgat tttgtgagtg aacaggagga cgaggaatga catataaact tgtaatcgcg
    31981 gaaaaaccca gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac
    32041 ggctatttgg aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt
    32101 gccgacgccg gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt
    32161 ctcccggaaa catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc
    32221 tgttcgctga tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc
    32281 gagggggaat tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag
    32341 cgcctttgga tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa
    32401 ccgggcggcg actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg
    32461 ctgatcggca ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg
    32521 ggccgcgtcc agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat
    32581 ttcaagaaag agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc
    32641 agccgctttt ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag
    32701 gccgtttgcg tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac
    32761 cttaccacct tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt
    32821 gactatgccc aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa
    32881 tatcttaccg acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg
    32941 gtgccgtttg ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa
    33001 aaggtgtccg accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc
    33061 actctggccg agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc
    33121 gtggccgggc cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag
    33181 tttaccgcca agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc
    33241 cgttcttcct taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag
    33301 ctttgcgagg gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg
    33361 ccgcccaagc cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag
    33421 gatatgccgg aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt
    33481 ctggaaaagc tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc
    33541 aaggacggca tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact
    33601 gccgaatggg aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt
    33661 atggacggca tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag
    33721 gaaaagcaga aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt
    33781 gaggctgtct atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc
    33841 atgtggaaga atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc
    33901 gctgcgctgc tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc
    33961 aaaacctacg acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc
    34021 attgaacaca ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc
    34081 cggtaaaatc ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc
    34141 cccaaccccc tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt
    34201 ttctatccaa gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga
    34261 tttgcccacc acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga
    34321 caatccgcag gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat
    34381 tccctatgac atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct
    34441 gggccagctt gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg
    34501 cggctttgag aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca
    34561 tcttcccgac gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat
    34621 ggtggatatg ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat
    34681 tgcccagcag gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga
    34741 atggcagcgc gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc
    34801 ccagccggaa gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc
    34861 cgacaaagcg ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg
    34921 catgaaagtg attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg
    34981 ctatatggct tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct
    35041 gctgatcgcc atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt
    35101 tgaccgccat gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa
    35161 ggttaagcag cagatggaga aaatcgaccc ggccacccag cagcccgtga tcggcgcgga
    35221 cggcaagccc gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt
    35281 ctttgacatc agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac
    35341 cggcgacgtg cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc
    35401 cataagcatt gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg
    35461 cattgccatc aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat
    35521 tgcccatgcc aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg
    35581 gccagaccgc agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta
    35641 ttacgggctg gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag
    35701 ggagttatcc gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat
    35761 aaccgagatc gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca
    35821 ggaggccgtc acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga
    35881 acaggccccg gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag
    35941 cccccagcta aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg
    36001 tctggatgca gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg
    36061 tatctttcag ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg
    36121 ttccctgatc gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa
    36181 aaagcatgta atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga
    36241 tatgctgtta ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga
    36301 acagacggcg cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta
    36361 tttgggggcg cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta
    36421 tcagttgccg gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc
    36481 acaggtacag gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata
    36541 cgcggcggct ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg
    36601 cggggaggaa acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct
    36661 ggccgtggac agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc
    36721 tttggaggat atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag
    36781 cctttccgtt tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt
    36841 agacagcttc ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc
    36901 cgaactgtct acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc
    36961 cgaaccgcag ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg
    37021 ccagacacag gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc
    37081 catcaatgag ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc
    37141 cggcagcgcc acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg
    37201 ccagaaaaag cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta
    37261 cgcccgcaag ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc
    37321 tgtcctgatt gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc
    37381 cagtgaccgc aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag
    37441 caccggcatg ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa
    37501 actggaaaag ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa
    37561 gcatgggacg ctggacggat gcccccatct gaccgaaaga ggcattgaaa acctgaaagc
    37621 ggatatggcg tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctctccaa
    37681 caataacgcc gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga
    37741 ggcggcctat gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa
    37801 ccgcctgcaa atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag
    37861 caacggtttc cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac
    37921 aatccgcgtc gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc
    37981 cttacagctt gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc
    38041 ggccaagacc gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt
    38101 agagtaaatg cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg
    38161 gcagaacacc gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga
    38221 tgccgccagc gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac
    38281 gtactgtttc cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa
    38341 attttacggg gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac
    38401 acagggcggc actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca
    38461 cgtcattgaa accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg
    38521 tgacatcccg gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt
    38581 gtactatgag gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca
    38641 gcacagccgg gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga
    38701 cagccggatt gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc
    38761 taacagcccc aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc
    38821 ttccaccaag gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac
    38881 cgggatgaaa ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt
    38941 gagtattcgc aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc
    39001 cagcccaaaa aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat
    39061 gcactttacc gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag
    39121 aacgataaac aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc
    39181 ccagcagacc ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt
    39241 ccacttcaca gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg
    39301 cgtccggcgt cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga
    39361 taggatgaag tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt
    39421 ttcaatccct taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg
    39481 cgcagcgggt gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg
    39541 gtgcagccct cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag
    39601 tttcagggaa atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc
    39661 ctgtctgatc tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt
    39721 ttccgtggcc cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt
    39781 ttcttgtccg tccatcctga tacccccgtc agactgattt tttctatgat accatattcg
    39841 gaagcatgga accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca
    39901 gcacttcggc ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc
    39961 ttgaaatata agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg
    40021 cgctcggcct ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg
    40081 ttagacttcg gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct
    40141 ttggtgatct cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg
    40201 cacagaagcg aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac
    40261 aggcagttgc cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat
    40321 tgagggtaat tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca
    40381 tacgcaaaat gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg
    40441 cttcgccgta cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt
    40501 tcagggcgta cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag
    40561 cttcatacaa aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat
    40621 tgcggtattc catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca
    40681 ccgccatatc cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg
    40741 cggcggtgat ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag
    40801 tcaggaaatt caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat
    40861 agcctgccga ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct
    40921 tgcgctcttt gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct
    40981 cggtgtaggc ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga
    41041 aagaaataaa ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat
    41101 catcaaaatc ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc
    41161 agctcgtccc tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg
    41221 gcttcaccac ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg
    41281 catagctctg tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa
    41341 taatgtgatt gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc
    41401 cttgcagcac ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt
    41461 cgccgggggc aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct
    41521 gcgccagcag catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca
    41581 ccagcagctt gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg
    41641 ttgacttgat aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc
    41701 ctcgatgtcc tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac
    41761 gttgacgccg atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt
    41821 gacgatatag ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag
    41881 cagcgccatt ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac
    41941 gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt
    42001 tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag
    42061 tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag
    42121 ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta
    42181 cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca
    42241 ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg
    42301 tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc
    42361 cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt
    42421 atgacaaccg gcagactggg aatggccccg tcgttttcga tgttggcaag atagcgccag
    42481 tcaatattta ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct
    42541 ttcacatccg cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac
    42601 ctcccactta cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc
    42661 ttaaagttta tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta
    42721 ccctgtggag gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg
    42781 tgcccccacg atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga
    42841 tggcgacgat ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga
    42901 aaggaaaggc ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa
    42961 gagagagaaa gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc
    43021 tccagaccga taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt
    43081 atgaagaagc gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa
    43141 gctgctgctg tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat
    43201 ggtgaagtct ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat
    43261 tgattttaat accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg
    43321 cgattattac atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga
    43381 cttgctgatt ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg
    43441 caagagcgac cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg
    43501 tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa
    43561 aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat
    43621 ccattgcttg ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa
    43681 cattaagaag gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga
    43741 tacatacacc atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa
    43801 atcgctttga aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga
    43861 tttttatgtc gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa
    43921 gggcgtttct ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat
    43981 acaccaagtt ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta
    44041 tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga
    44101 tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact
    44161 atgtaacaaa gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc
    44221 gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt
    44281 tcacggtact ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt
    44341 tgctgctggt gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat
    44401 ggggatatga ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg
    44461 ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat
    44521 cataataatg gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct
    44581 gctactggtt gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag
    44641 agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa
    44701 tagcgatagg cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt
    44761 tgagttttat attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac
    44821 atcaaatagt tttagacccg actttcttta tgtggtacat agagatgata atatttcgat
    44881 tgttgctcaa aacaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat
    44941 tgttatgatt gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca
    45001 aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga
    45061 tgtaccgccg ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc
    45121 catgtatgtc aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca
    45181 tgagttggag gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc
    45241 catcgcggct acaagcgttc tgttggaggg aatgcttgga aatatcggtg actacaaaga
    45301 tcattctaag tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc
    45361 cgaaatactg gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt
    45421 ggacataggg cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa
    45481 caaccagcgg ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct
    45541 gctccaaaag gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg
    45601 cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat
    45661 gggcgttcac attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga
    45721 tcaggcgcga agcagaaaaa gtgggcgaag cggtttagga cttgccatcg tacaaaaaac
    45781 gctggatgcc atgagcctcc agtatgcgct ggaaaacacc tcggatggcg ttttgttctg
    45841 gctggattta ccgctcacat caacattgta aatatttaaa acttaaatga ttttgaccga
    45901 caggtataac cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg
    45961 gaaagtacag atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt
    46021 gcaaaggagg cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac
    46081 aaacggcata tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg
    46141 ttcacagtct gcacgctgtt tttggggtgg agactggctt ccgtattgga ggcaacacag
    46201 ataccgccca tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag
    46261 gaaaacgctc ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta
    46321 gtgaacaggc agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat
    46381 ggtgagcgga tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga
    46441 actgatggag tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa
    46501 attatggatg aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa
    46561 gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct
    46621 gtggatatca atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat
    46681 gaaaacagct atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc
    46741 ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg
    46801 tacaaccaag ggggttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt
    46861 atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg
    46921 cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga
    46981 cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc
    47041 acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag
    47101 caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc tggtggctgg
    47161 tacgccatgc ggacaaagat accccctata aagacggcct tacggtgacc aatggtaaac
    47221 tcaccaccat gtcgggcggc ggtatgtgcc agatgagcaa tttactattt tggatgttcc
    47281 tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc
    47341 caaacagtga cgagattaaa ggggtggatg caaccattcc agagggctgg attgatttaa
    47401 aagtgcgaaa cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa
    47461 tcatcggtca ggtgtccgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca
    47521 gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc
    47581 aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca
    47641 aaatctgtta tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag
    47701 tgtgggcatt actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc
    47761 gccggatttt catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa
    47821 attggccgct ggcaatcaat gcgttagcgt aggccataag tccgaggttt ccgaggcgac
    47881 aattcttgcg ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga
    47941 tcacattgat acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc
    48001 gccggacagc gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa
    48061 acccaccatg cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga
    48121 actgggggat atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa
    48181 aaggctgcgg ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc
    48241 agattatgtc cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc
    48301 gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg
    48361 cgcattttta atcaacaccg cgcgcggagc gcttgtcgat accggggcgc tggtggaggc
    48421 gttggaaagc ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt
    48481 tgtttatacc gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag
    48541 gatgccgaat gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga
    48601 tactacagaa aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata
    48661 gaataaaagt cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaat
    48721 ccgcaataga aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa
    48781 ttacaaaaaa cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca
    48841 gtctccccgc catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa
    48901 gcgaatacga aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaattcgggg
    48961 aggatggtgc gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata
    49021 ttcaaagctc cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg
    49081 gcatcgccgt tcccgaattt caaattattg ataaaggtga caagccggag gcgggtgcgc
    49141 ttacctaccc tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag
    49201 taaacggtac ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa
    49261 tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg
    49321 atgatttgat tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc
    49381 atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca
    49441 ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg
    49501 gatgcagagg gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa
    49561 atgaggtcaa taccatgccc ggttttacat cgtacagccg ctacccacgt atggtggccg
    49621 ccgcaggaat cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt
    49681 gataagtatg gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga
    49741 tgccaagtac gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa
    49801 tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc
    49861 tgcgcttggc tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg
    49921 cttcctgcgt tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa
    49981 tatagagcga gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg
    50041 tggaagcgca attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg
    50101 aagtaacttc gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc
    50161 agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta
    50221 tcgctttgaa tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa
    50281 ttttgccgtt tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct
    50341 atgctggtca tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg
    50401 caaattatag tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac
    50461 ctttcccacg cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt
    50521 cttttctggc agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta
    50581 ttgccatgct ctaatccact tccaccactc aaaaaagcct tgcccctcca tgaactgaac
    50641 cccaaaagtt gaactattta atggactgt
//
LOCUS       DAJSNV010000298        17782 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.054, whole genome shotgun sequence.
ACCESSION   DAJSNV010000298 DAJSNV010000000
VERSION     DAJSNV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.054"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            60..977
                     /locus_tag="ON516_003075"
     CDS             60..977
                     /locus_tag="ON516_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3346465.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            980..3157
                     /locus_tag="ON516_003076"
     CDS             980..3157
                     /locus_tag="ON516_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3346466.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3154..4155
                     /locus_tag="ON516_003077"
     CDS             3154..4155
                     /locus_tag="ON516_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3346467.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4152..5084
                     /locus_tag="ON516_003078"
     CDS             4152..5084
                     /locus_tag="ON516_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3346468.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5329..5445
                     /locus_tag="ON516_003079"
     CDS             5329..5445
                     /locus_tag="ON516_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3346469.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5461..7380
                     /gene="tet(M)"
                     /locus_tag="ON516_003080"
     CDS             5461..7380
                     /gene="tet(M)"
                     /locus_tag="ON516_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3346470.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7499..7666
                     /locus_tag="ON516_003081"
     CDS             7499..7666
                     /locus_tag="ON516_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3346471.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7726..8079)
                     /locus_tag="ON516_003082"
     CDS             complement(7726..8079)
                     /locus_tag="ON516_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346472.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8584..9006
                     /locus_tag="ON516_003083"
     CDS             8584..9006
                     /locus_tag="ON516_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3346473.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            9003..9233
                     /locus_tag="ON516_003084"
     CDS             9003..9233
                     /locus_tag="ON516_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3346474.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9694..9897
                     /locus_tag="ON516_003085"
     CDS             9694..9897
                     /locus_tag="ON516_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3346475.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9979..11196
                     /locus_tag="ON516_003086"
     CDS             9979..11196
                     /locus_tag="ON516_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3346476.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11428..11754
                     /locus_tag="ON516_003087"
     CDS             11428..11754
                     /locus_tag="ON516_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3346477.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11822..12535
                     /locus_tag="ON516_003088"
     CDS             11822..12535
                     /locus_tag="ON516_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3346478.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12845..13696
                     /locus_tag="ON516_003089"
     CDS             12845..13696
                     /locus_tag="ON516_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3346479.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13817..15682
                     /locus_tag="ON516_003090"
     CDS             13817..15682
                     /locus_tag="ON516_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346480.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15730..17139
                     /locus_tag="ON516_003091"
     CDS             15730..17139
                     /locus_tag="ON516_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3346481.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            17247..>17782
                     /locus_tag="ON516_003092"
     CDS             17247..>17782
                     /locus_tag="ON516_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3346482.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFS"
BASE COUNT         5905 a         3059 c         3761 g         5057 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
      181 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
      301 aattacgggt tgagaatacg ccactaagta ccagtatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcacat ttattattca gtaatggtta tgtggagcag tctatcagct
      421 tagaaaaaca actgaacatt atacaggttg cggacttggt acttcccgac aaggaaactt
      481 cctttgagga atataccact atggagcttt tatccgttgc tatgctgatt gtcattagta
      541 cctttgcttt agactttatc catacagacc gaagcatttt caagattgta gatttagacg
      601 aagcatggag ctttttacag gtagcacaag gaaaaacact atctatgaag ctggttcggg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagac gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcatt tcgttccact gaccttaacg
      781 agattaaaaa gaccttagcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatagtaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggcttc cgtggaactg gtcggacggg attggaaaac aggtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ttgtatgttt ccaatgcgac
     1321 aggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
     1441 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
     1501 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
     1681 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
     1801 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
     2401 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
     2461 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
     2521 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
     2581 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
     2641 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
     2701 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
     2761 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
     2821 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
     2881 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
     2941 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
     3001 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
     3061 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
     3121 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
     3181 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
     3241 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
     3301 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
     3361 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
     3421 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
     3481 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
     3541 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
     3601 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
     3661 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
     3721 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
     3781 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
     3841 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
     3901 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
     3961 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
     4021 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
     4081 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
     4141 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
     4201 aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cataaaaagg
     4261 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
     4321 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
     4381 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
     4441 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
     4501 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
     4561 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
     4621 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
     4681 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
     4741 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
     4801 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
     4861 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
     4921 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
     4981 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
     5041 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
     5101 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
     5161 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
     5221 aaggtattat taaataaact gtcaatttga tagcgggaac aaataattgg atgtcctttt
     5281 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
     5341 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
     5401 aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac
     5461 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
     5521 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
     5581 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
     5641 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
     5701 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
     5761 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
     5821 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
     5881 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
     5941 tatcctaatg tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
     6001 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
     6061 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
     6121 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
     6181 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
     6241 aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg
     6301 gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt
     6361 gaattatgta agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt
     6421 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
     6481 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
     6541 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
     6601 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
     6661 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
     6721 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
     6781 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
     6841 gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg
     6901 atacgctatg gttgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
     6961 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
     7021 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
     7081 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
     7141 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
     7201 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
     7261 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
     7321 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
     7381 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
     7441 taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt
     7501 gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa
     7561 attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa
     7621 agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac
     7681 aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt
     7741 tcttgcttcg ttgataccgc tggctaaaga ttccattatg gatagttctt tgtctgtaaa
     7801 gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg
     7861 taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat
     7921 gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa
     7981 tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc
     8041 aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt
     8101 ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg tttataggtt
     8161 tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat ggtagtgcta
     8221 tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc
     8281 gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc aaaaaaagtc
     8341 gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat atagatagaa
     8401 taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg ctattcagaa
     8461 cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca ttgggactaa
     8521 gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa gggggtgaga
     8581 aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta tatctgtaaa
     8641 cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat tgcaaagcgt
     8701 gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac aacagataac
     8761 tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt tgaaaatgat
     8821 ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct actgctgttt
     8881 tactttatgg acatgagcga ttcagaaatt gcagacctgt tgaaattgaa ccgttctact
     8941 gtctatcggc atagaaccag tggactagcc ttaattaaaa agtttatgga ggaatttgaa
     9001 gaatgaaaac acaatatcct atgattccct ttcctctcat tgtaaaggca acagatggcg
     9061 ataccgaagc gattaaccag attctacatc attacagagg gtacataacg aagcgttccc
     9121 tacgacttat gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg
     9181 gaagaatgga aaccagactg attacaaaga ttttgtcatt tgaaattaag taatatcctc
     9241 tctcctttcg tggaagcgtg ctaaaccatt ccacgcttcc cgaacaggga ggtttgttat
     9301 tccaccaaag catattgagc tttcaatgtg ttttgatagg ctaacgagcc attgttcttt
     9361 gaaaactgaa taaaagtaat cgaatacgtt tcgataagaa aagagccaac ggaactaacc
     9421 gccatgacct atcttataaa gatagcgagc gattcatgtt agtgatccga gaagcaatct
     9481 ttagcaggat tgcctgcaac gacattctta tcgtgataat gatactccca tacagtcaat
     9541 agtccgagcg tgataaaacc gtcgcaggca atgagtatgg ctacatgaga accatgcagg
     9601 ggtggaactc ccgtgagctt tgctaaagct gttcgattgc tggtaaaaca acttttatga
     9661 aatccaaata agtgatttgg aaaggaggat tttatgaagc agactgacat tcctatttgg
     9721 gaacgttata ccctaaccat tgaagaagcg tcaaaatatt ttcgtattgg cgaaaacaag
     9781 ctacgacgct tggcagagga aaataaaaat gcaaattggc tgattatgaa tggcaatcgt
     9841 attcagatta aacgaaaaca atttgaaaaa attatagata cattggacgc aatctagcgt
     9901 cgccaaaggg tcttgtatat gataaaatag tattaagtcg tatcaaggct ctttccataa
     9961 aggaaaggag caaatgccat gtcagaaaaa agacgtgaca atagaggtcg aatcttaaag
    10021 actggagaga gccaacgaaa agacggaaga tacttataca aatatataga ttcatttgga
    10081 gaaccgcaat ttgtttactc gtggaaactt gtggctacag accgagtacc agcaggaaag
    10141 cgtgattgta tctcacttag agagaaaatc gcagagttac agaaagacat tcatgatggt
    10201 attgatgttg taggaaagaa aatgacactc tgccagcttt acgcaaaaca gaacgctcaa
    10261 agaccaaagg ttagaaaaaa cactgaaact ggacgcaaat atcttatgga tattttgaag
    10321 aaagacaagt taggtgtaag aagcattgac agtattaagc catcagacgc taaagaatgg
    10381 gctattagaa tgagtgaaaa tggttatgct tatcaaacca tcaataacta caaacgttct
    10441 ttaaaggctt cattctatat tgctatacaa gatgattgtg ttcggaagaa tccatttgac
    10501 tttcaactga aagcagttct tgatgatgat actgtcccta agaccgtact aacagaagaa
    10561 caggaagaaa aactgttagc ctttgcaaaa gctgataaaa cctacagcaa aaattatgat
    10621 gaaattctga tactcttaaa aacaggtctt cgtatttcag agtttggtgg tttgacactt
    10681 ccagatttag attttgagaa tcgtcttgtc aatatagacc atcagctatt gagagatact
    10741 gaaattgggt actacattga aacaccaaag accaaaagtg gcgaacgtca agttcctatg
    10801 gttgaagaag cctatcaagc atttaagcga gtgttagcga atcgaaagaa tgataagcgt
    10861 gttgagattg atggatatag tgatttcctc tttcttaata gaaagaacta tccaaaagtg
    10921 gcaagtgatt acaacggcat gatgaaaggt cttgttaaga aatacaataa gtataacgag
    10981 gataaattgc cacacatcac tccacatagt ttgcgacata cattctgtac caactatgca
    11041 aatgcaggaa tgaatccaaa ggcattacag tacattatgg gacatgctaa tatagccatg
    11101 acgctgaact attacgcaca tgcaacattc gattctgcaa tggcagaaat gaaacgcttg
    11161 aataaagaga agcaacagga gcgtcttgtt gcttagtagt acaaatgaat ttactactta
    11221 tttaccactt ctgacagcta agacatgagg aaatatgcaa agaaacgtga agtatcttcc
    11281 tacagtaaaa atactcgaaa gcacatagaa taaggcttta cgagcattta agaaaatata
    11341 aaaagataat tagaaattta tactttgttt tatgtaaaaa aatggtatga ttaatcaagt
    11401 aagaagtgat tggaagaagg caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga
    11461 gatgaacgta attggcgaca atttcataac gaaaaagatt tggctatctc tatttctttg
    11521 gaagcatctg aattgttaga actttttcaa tggaaaacgc cggaagaagt aaaagaaaca
    11581 caattagaac gaatcaaaga agaactggca gatgtattga tctattcata catgatggca
    11641 gacaaccttc acctggatct agatgagatc attgaagaaa aattgatcaa aaataatttg
    11701 aaatatcctg tagaaaaaag cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt
    11761 gtcattcggc tgttggaaag agcaaaagaa aaacagtaaa atgagtctgg agggagagaa
    11821 aatgaaaaaa aaaggaaaag caaaaagatg gctagtcaat atcctcttgt tcctcttgtt
    11881 attagtagga ctagctttga ttttcaatga acagatcaag gattattttg tcagagagac
    11941 aggagataaa tatgctatag cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa
    12001 agatgtcagt tttgactttg atgcggtaga accaatgacg acagaagggg tcatgaggtc
    12061 gcaaatgaga ggaacagatc taccagtgat tgctagtatc gcagtccctt cagtttcaat
    12121 caatttaccg gttttcaaag gcttggataa tacgtcacta ctttacggag caggaacatt
    12181 atctccagat caagaaatgg gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa
    12241 ccctgaattg ctgtttacac cgctagaaaa tttagagatg ggtgccaaga tctatttgac
    12301 agatttagaa aatgtgtata catacaaaac attttttaaa gaaaaagttg ctcctacaga
    12361 tacccaatta ctgaatgaag tcgagggaaa agaaattgtc acactaatta cttgtggcga
    12421 catggacgcc gtcacgcgac tagttgtaca aggagaactg gaaagtgtta catcgataaa
    12481 agatgcgaca gatgatatga gatctgcttt caatctagag acaaaaacat tttaggatac
    12541 tgggacataa gtcgttacag attaaaaaca gccaaaggat atgaatcgat aaggaaaaac
    12601 caatttgctg aaaaaaagag aaacgcaaag aggtgcatct ctgatttggt tgttttctag
    12661 atagacttgt gttccagttt ttttattttt tatatgaaaa ggcttgcata aataaaaatt
    12721 cgatggtatg atgattttga ataagaaatg gattgttact gtacggcagg caaaaccaga
    12781 attcagctaa gaaacagtag gcgaattcta ggttttttta tttttcttga gagggcgaag
    12841 caaaatgaaa attgataaaa ttttgaataa caatgtcgtc atttcgaaaa atggattcgg
    12901 ggaagaagtc gtttgtatgg gaaaaggact agcttttcaa aaaaagattg gggacgagat
    12961 ttcgccagaa gcggtccaaa aagaatttgt tttaagagat tcttttgcta aaaaccaatt
    13021 ccagcagctg atggctgata ttccggcaga ggagatcgaa tgggtcaaac aagtagtcga
    13081 actggctgaa gataaactaa aagttgaatt ttcgcctaat atttatctga cattgaccga
    13141 tcatcttcat tatgcgatca cacgagcaaa agaaaatatc caactgccta atccattatt
    13201 atttgaaaca aaaaaatttt atccgaaaga atatcaagca gcgaaagagg ctcttttgtt
    13261 cgtgaaacaa aagacgggtg tggaactttc tgaggatgaa gccggtttta ttgcctttca
    13321 ttttgtgaat agccaacaag gaaacgaaga catgcaagtg acgatgaccg ctaccacaat
    13381 ggtaagagat atactgagta tcatcagtaa attcttcggc atcgtgttcg atgaagaatc
    13441 tttgaattat cagcgtatca ttactcatct gcagtttttt gcccagcgtt atctcaaagg
    13501 ggaaggatct gacgagcaag atgaatttct ctacgcatta gttcaaggaa agtatccaaa
    13561 agcgtttcat tgtgtcgaac ggatcaatga gtatcttcga caaacgcaaa aacaggaaat
    13621 ggggatcgca gagcagattt atttaacgat tcatattcaa agagtagtaa gtgagaaaaa
    13681 aagcatccaa tcataaggat tgttactgta tggcaggcaa aacctggatt gaaaaaatag
    13741 gttcacgaat gtttgtgact gtttttttca atctgggttt ttatttttta aataaaaaag
    13801 aaagaaggaa aaaacaatgg ataatcaagc agttggtcgt cgtgtatggg aagcggtagg
    13861 aggacaaaag aatgtcaaaa gtttagtaca ttgtgctacg cggttgcgat tcagattaaa
    13921 agatgaatct ttagcagata cgcaaaagtt aaaagaagac ccggacgtga tccaagtcgt
    13981 acaaagtggc ggtcagtatc aagtagtgat tggcagtaat gtagcagatg tttatcaatc
    14041 gatcgtggat gaggaaggtc ttgcagtttc ggaggatgga aaagaagagt ccaaaaatat
    14101 tttgaatcgt ctgatcgata ttatctctag tatatttaca ccttttctag gggcgatggc
    14161 agccgccggg gtattgaaag gattcttatc attggcaacg gtgatgggct ggctgacagc
    14221 tgatagcggt acttatcaaa tattgtttgc tgcagcagat ggtgtcttta ccttcttacc
    14281 tgtcatgctt gcttttacag cagctaaaaa gttcaaagcc aaccagtttt tagcagtagc
    14341 aattgctatg gcacttgtgt atccagctat cacagcagtt gctggtgcgg gagaagctat
    14401 cagtttcttt gggatcccag tgattctttc accatctggt tatacttctt ctgtcatacc
    14461 aatcattcta gccgtatggg ttcaaagtaa attagagcca ttcgtaaaaa aagtgattcc
    14521 gcaatttctg caaatgattt tagttccgct tgtcgtttta gtagtgatgg ttccattaac
    14581 atttcttgct ttaggaccaa tcgggacggt tgccggtaat gctttaggcg gtctcttcaa
    14641 ctcgatttat ggatttagtc caatcgtagc aggtctaatc atgggaagtt tatggcaagt
    14701 attcgtgatg ttcggcatgc attggggatt cgtaccgatc atgtttttga atatcgaaca
    14761 atatgggttt gatgtattga tgccaatgct tcttccagcc atcttggcac aaggtggggc
    14821 tgctttagct gtagcattgc gaacaaaaga tacgaaactt cgagcattag ggatttcttc
    14881 tacagtgact tcgttatttg gaatcactga accaactgtg tatggtgtga cattaccttt
    14941 gaagaaacca ttcattgccg cttgtatttc tggaggtatt ggtggagcta ttatcggatt
    15001 ttccggagta aaagctttct ccagcagttt ggtcagctta ttgacgattc cgactttcat
    15061 taatacggtg gatggtgtag agtcaaatgt gacagtagca gtaatagcta caggaattgc
    15121 ttttgttctt gcattcgtag gtacattgat tttaggattt gacgaacaaa cacaagacaa
    15181 tcaattagaa aacaaacatg cgaatgcagg agaaccgatt acttcagcac ggcatacctt
    15241 aaaaagtcct ttaacgggta aagtactgcc attgagtgaa gtaccagacc aagttttttc
    15301 ttctggcgtg atgggaaaag gtatcgctat tgatccagaa gtaggagaac tagttgcacc
    15361 tgcagacgga gagatcacga ctatttttcc aactggacat gctgtaggga tcacaacgac
    15421 tgacggagcg gagattttga ttcatatcgg gatggataca gtagaattaa atgggaatgg
    15481 ctttgagatc ttagtcaaac aaggggatct cgtcaaagca ggagatttat tgattcgttt
    15541 tgacatcgaa gcaattagag cagctggcta tagcgtaatc actccagttg tcattacgaa
    15601 tactgatgca tttgccgata tacttgaatt agaccaaaaa gagattattg caaacgaaga
    15661 tgttctggca attgtaaaat aaggaggaaa tcaggatgca atcacgtttt tcagaaaaat
    15721 ttttatgggg tggagcaact gctgccaaat caattagaag gggcttatga tcgagacgga
    15781 aaaggactat ccgttgcaga tgctatgcct ggaggtaagc agcgctttgc gattatcggc
    15841 agtgaagaat ttgactggac gattgatcaa gaaaaatata tttacccgaa tcatcgagga
    15901 atcgatcatt acgatcgttt caaagaagat cttgccttgt ttgcggaaat gggattcaaa
    15961 tgttatcgtt tttcgattgc ctggacacga atttttccaa atggagacga aggtactcct
    16021 aacgaagcgg gattagaatt ttatgatcag ctgattgatg agtgcttaaa atatgatatt
    16081 gaacctgtga tcacgatttc tcattacgaa atgcctcttc atttggcaaa agaatacggt
    16141 ggctggaaaa atcgaaaact gatcgacttc tatgaacgat ttgctcagac agtattagag
    16201 agatacagca gtaaagtcaa atattggatg actttcaatg aaatcaattc ggcctttcac
    16261 tttccagctt taagtcaagg attagtaaaa agcaatggag caggagagta ccaaaatatt
    16321 ttccaagcat ggcataatca attcgttgca agtagcaaag ccgtaaaaat cggccacgaa
    16381 ttgcgttcgg atatccaaat cggctgcatg attatctatg caacaactta cagcatcgat
    16441 gccaatccag tcaatcaggc agcaacaatg atccaaaacc aagaattcaa ttttttctgc
    16501 acggatgtcc aagtacgtgg agaataccct gcatacacag cgcgtaccca taaaaaatat
    16561 ggagtcgatc cggaaaaatt agagcaaacg gaaaaagact tccagttgct aaaagaatat
    16621 ccagtggact acatcggatt tagttattat atgtctacgg caatcaatga gactgatcca
    16681 caagcagcaa cttcagaagg gaacttgctg ggaggagtaa aaaatccgtt tcttgaggca
    16741 agtgaatggg gctggcagat cgatccagaa gggctgagaa tcgcattgaa tgaattatac
    16801 aatcgttacc agaaaccatt attcattgtt gaaaacggtc ttggagccat tgatcatatg
    16861 gaagaggatg gcacgattat tgatgattat cggatcgatt atttacgtcg tcatatcgaa
    16921 gcgatggcta atgcagtagc agacggtgta gatttgatgg gctatacgcc ttggggatgt
    16981 atcgatcttg tcagtgcatc aacaggagaa atgagtaaac gttacggctt tatctatgtg
    17041 gatctagacg atgaaggaaa tgggacactg aatcgatcaa agaaaaaatc ttttgattgg
    17101 tataaagaag taattgcctc aaatggcgta aacttgtaat taaagcagct gaatgaaaaa
    17161 gaacacactt ctcacatgtg atttgtgttc tttttttatt ttttatgtgc tattctttta
    17221 gtaatagtaa agcaaagggt gacgttatga aaaggacacg tgtactatac ttggaagtcg
    17281 cagataaaat taaagaagat attttctcag gaaaatatcc agtcggttcg atgttgccga
    17341 cagaaagtga attagaagaa ctgtttcaag tcagcaaaat cactgtccga aaggcaatcg
    17401 aactgctggc atcagatgaa tatgtcgcaa agaaaagtgg tcgtgggacg acggttctaa
    17461 gcaatcgtcc ttacaatcga ttatccaaag cggcaagctt tacgcaaatc ttggagaact
    17521 cccgatatga agtccggaaa gaaacgctgg atttgcaatt ggtccatata aagaaggaag
    17581 atcctttgtt ttcctttttc ggtgagcaag ctgtttgttt taagcgctta tattatttga
    17641 acaatcagcc ttatatttat tttgttcatt atcttccaca agaaatgatg caattcagca
    17701 gagaagaatt tggcaaagaa tctttgtatc gtttgctgac gaaaaaagga tatactattg
    17761 actcattttc tgatgatttc tc
//
LOCUS       DAJSNV010000299        14778 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.064, whole genome shotgun sequence.
ACCESSION   DAJSNV010000299 DAJSNV010000000
VERSION     DAJSNV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.064"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            117..1013
                     /locus_tag="ON516_003093"
     CDS             117..1013
                     /locus_tag="ON516_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3346483.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1163..2104)
                     /locus_tag="ON516_003094"
     CDS             complement(1163..2104)
                     /locus_tag="ON516_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3346484.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2230..3351
                     /locus_tag="ON516_003095"
     CDS             2230..3351
                     /locus_tag="ON516_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3346485.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3609..5111
                     /gene="eat(A)"
                     /locus_tag="ON516_003096"
     CDS             3609..5111
                     /gene="eat(A)"
                     /locus_tag="ON516_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3346486.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5139..5705
                     /locus_tag="ON516_003097"
     CDS             5139..5705
                     /locus_tag="ON516_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3346487.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5916..6977
                     /locus_tag="ON516_003098"
     CDS             5916..6977
                     /locus_tag="ON516_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3346488.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6980..7876
                     /gene="murQ"
                     /locus_tag="ON516_003099"
     CDS             6980..7876
                     /gene="murQ"
                     /locus_tag="ON516_003099"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3346489.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7890..9872
                     /locus_tag="ON516_003100"
     CDS             7890..9872
                     /locus_tag="ON516_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3346490.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9937..10383)
                     /gene="nrdI"
                     /locus_tag="ON516_003101"
     CDS             complement(9937..10383)
                     /gene="nrdI"
                     /locus_tag="ON516_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3346491.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10594..12018
                     /locus_tag="ON516_003102"
     CDS             10594..12018
                     /locus_tag="ON516_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3346492.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12199..12972)
                     /locus_tag="ON516_003103"
     CDS             complement(12199..12972)
                     /locus_tag="ON516_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3346493.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12969..13874)
                     /locus_tag="ON516_003104"
     CDS             complement(12969..13874)
                     /locus_tag="ON516_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3346494.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14028..14348)
                     /locus_tag="ON516_003105"
     CDS             complement(14028..14348)
                     /locus_tag="ON516_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3346495.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4737 a         2622 c         3114 g         4305 t
ORIGIN      
        1 catattttca tacaaatgtt aaattttcat acaaatatag gaaacgtttt actggctttt
       61 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga
      121 acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg ccttccatcc
      181 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagcta
      241 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      301 tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt tggaatcact
      361 tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      421 tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat agttcatttt
      481 attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      541 cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata aaaagagatg
      601 ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga tatcttactt
      661 atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg ttgcgggagg
      721 gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag aaactagtga
      781 cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt tttttgcttc
      841 gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag ccaatcctat
      901 cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc actaacgatt
      961 ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct
     1021 acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc atcatgagag
     1081 ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag
     1141 ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta
     1201 attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa
     1261 tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca
     1321 tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca
     1381 cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga
     1441 ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca
     1501 aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac
     1561 gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt
     1621 ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca
     1681 atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat
     1741 ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca
     1801 aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat
     1861 tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg
     1921 gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat
     1981 ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc
     2041 tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt
     2101 tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga
     2161 aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga
     2221 gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt
     2281 gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg
     2341 ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca
     2401 tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg
     2461 acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat
     2521 ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa
     2581 cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc
     2641 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
     2701 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
     2761 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
     2821 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
     2881 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
     2941 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
     3001 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
     3061 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
     3121 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
     3181 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
     3241 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
     3301 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
     3361 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
     3421 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
     3481 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
     3541 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     3601 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     3661 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     3721 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     3781 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     3841 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     3901 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     3961 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     4021 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     4081 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     4141 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     4201 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     4261 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     4321 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     4381 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     4441 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     4501 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     4561 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     4621 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     4681 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     4741 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     4801 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     4861 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     4921 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     4981 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     5041 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     5101 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     5161 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     5221 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     5281 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     5341 ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga
     5401 ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag
     5461 atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag
     5521 tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa
     5581 atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc
     5641 cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
     5701 agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca
     5761 gtgccttttt taggttatga caattctttt gataatataa actcaacctt tagaaatgaa
     5821 atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat
     5881 agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga
     5941 acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg
     6001 tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat
     6061 tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag
     6121 gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg
     6181 gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac
     6241 atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga
     6301 aagaagcaga agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg
     6361 aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc
     6421 tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat
     6481 tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag
     6541 caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc
     6601 aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag
     6661 aattttacga atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca
     6721 tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta
     6781 cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga
     6841 tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta
     6901 tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa
     6961 gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag
     7021 aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa
     7081 caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt
     7141 gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg
     7201 aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga
     7261 accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga
     7321 aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa
     7381 aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt
     7441 agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga
     7501 aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg
     7561 ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat
     7621 cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa
     7681 tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga
     7741 aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat
     7801 tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt
     7861 aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac
     7921 tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact
     7981 gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa
     8041 aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac
     8101 ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag
     8161 aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg
     8221 taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa
     8281 tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg
     8341 gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac
     8401 ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt
     8461 ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa
     8521 ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac
     8581 tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca
     8641 tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg
     8701 cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt
     8761 tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg
     8821 tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg
     8881 ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg
     8941 gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat
     9001 tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt
     9061 taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag
     9121 gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga
     9181 tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct
     9241 ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg
     9301 ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg
     9361 aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg
     9421 tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc
     9481 cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata
     9541 tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga
     9601 tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag
     9661 gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg
     9721 tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct
     9781 tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag
     9841 caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa
     9901 gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat
     9961 ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg
    10021 aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt
    10081 atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc
    10141 acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa
    10201 ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg
    10261 gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata
    10321 ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt
    10381 catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg
    10441 tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt
    10501 atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt
    10561 gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga
    10621 attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt
    10681 ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc
    10741 gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca
    10801 gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt
    10861 atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg
    10921 ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg
    10981 atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa
    11041 aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt
    11101 attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac
    11161 attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc
    11221 atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca
    11281 aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt
    11341 tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc
    11401 aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc
    11461 gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta
    11521 ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa
    11581 ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc
    11641 tttttacttt cattattatt cactgacagt gcttataact ttgcttacac acttggtaca
    11701 gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag
    11761 cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct
    11821 atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt
    11881 tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat
    11941 aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact
    12001 gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt
    12061 tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca
    12121 gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc
    12181 ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa
    12241 gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa
    12301 ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt
    12361 ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga
    12421 agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc
    12481 aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca aagtataagt
    12541 gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg
    12601 gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga
    12661 ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata
    12721 aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc
    12781 aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg
    12841 atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa
    12901 tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac
    12961 aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat
    13021 tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc
    13081 tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct
    13141 tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag
    13201 ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc
    13261 tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga
    13321 ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct
    13381 cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg
    13441 ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct
    13501 tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca
    13561 tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc
    13621 gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca
    13681 ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg
    13741 atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag
    13801 ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc
    13861 gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg
    13921 agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata
    13981 ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta
    14041 gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga
    14101 ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct
    14161 gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa
    14221 aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca
    14281 ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc
    14341 catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc
    14401 ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat
    14461 ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag
    14521 tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc
    14581 atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct
    14641 atatattatc gggcaataaa ttaaaaaaag ctttacctta caataagtaa gataattttt
    14701 gtgataaaaa aataacgaaa aatatttaaa aatagtgtct atttcgtgag aaaacaacta
    14761 tattttttat gtgttttg
//
LOCUS       DAJSNV010000300         9847 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.099, whole genome shotgun sequence.
ACCESSION   DAJSNV010000300 DAJSNV010000000
VERSION     DAJSNV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.099"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(7..879)
                     /locus_tag="ON516_003106"
     CDS             complement(7..879)
                     /locus_tag="ON516_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3346496.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1007..1576
                     /locus_tag="ON516_003107"
     CDS             1007..1576
                     /locus_tag="ON516_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3346497.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1643..2380
                     /locus_tag="ON516_003108"
     CDS             1643..2380
                     /locus_tag="ON516_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3346498.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2611..3879
                     /locus_tag="ON516_003109"
     CDS             2611..3879
                     /locus_tag="ON516_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3346499.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4366..4830
                     /locus_tag="ON516_003110"
     CDS             4366..4830
                     /locus_tag="ON516_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3346500.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4814..5443
                     /locus_tag="ON516_003111"
     CDS             4814..5443
                     /locus_tag="ON516_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3346501.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5797..7275
                     /gene="msr(C)"
                     /locus_tag="ON516_003112"
     CDS             5797..7275
                     /gene="msr(C)"
                     /locus_tag="ON516_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3346502.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7322..7768
                     /locus_tag="ON516_003113"
     CDS             7322..7768
                     /locus_tag="ON516_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3346503.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7967..8482)
                     /locus_tag="ON516_003114"
     CDS             complement(7967..8482)
                     /locus_tag="ON516_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3346504.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8599..9801
                     /locus_tag="ON516_003115"
     CDS             8599..9801
                     /locus_tag="ON516_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3346505.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3130 a         1737 c         2052 g         2928 t
ORIGIN      
        1 ctctacttaa ctgtatttct ccgttaggac atctaaaaat ttacgagcag caggagaaag
       61 aggctggtct tttttccaaa tgagattcag tgaagcttgt aatggcggat cgagtgggat
      121 gaatactttt tccgtcccat aagtattgat gatcccatct agagctaatg caccaaaagt
      181 cccgttatcg accatcaaag aagcattgta caataagtta tatgttccga cgatcgataa
      241 ctgatctatg ctgaaaccta gccattcagc tagttgactg tccactgacg tttgccggga
      301 aatgatcaaa ggaacagttt tcagttcctg cggatggatg actgtttgct ttgctaaagg
      361 atgattccga tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa atgactggta
      421 tttttgtttg tttgtcggtt cgattacgag tccaaaatca agtaatccgc tatccagttt
      481 gtctatgata tcatctgcat tcccgctata taaatgaaac tggatatcag gatgcgcctt
      541 gatcaacgaa tgaaggctat cagaaatgaa agagattgct ttagtttcgc ctccgccaat
      601 gtagatttct ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa
      661 cgacaagatt tcttttcctt ttgaataaag atatcgacca gaatccgtca actgtatctc
      721 tcgggagcct cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt tcagttgttt
      781 tgagagagta ggttgcgtga tatgcagaga ttttgctgct cggctgatat ttttttctct
      841 agctactgct aaaaaatacg tcagtatacg aatatccatt ttttccctcc tgtttttaaa
      901 aaagcatacc actaagtata gttaaaagga atagttaagt atgttttata agtattagac
      961 atcataagcc gtccgttata aagtaaggat aaaaggaggg agcaaaatga caagcctcat
     1021 cgaaaaacaa atcgtcaata aagagattcg agtaacggat ccgctctttg aagtgatcca
     1081 tcagatccaa gcggaaaacg aagagccttt agcagcgctg aatactgggt accatgaacc
     1141 agcagatatc cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac
     1201 tgttctgctg cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt
     1261 tatcaatcgt caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt
     1321 gattggtcct cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag
     1381 aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac
     1441 tattctgcca ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac
     1501 gaaagatgtc ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt
     1561 cgaacggatt atttgaacag taagatgaaa taagagatag tgagtataag caagagattt
     1621 ttcaaaacag gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg
     1681 cgcttcaagc ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact
     1741 agttatagct gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc
     1801 tgagctatat attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga
     1861 tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc
     1921 aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat
     1981 catgggtgtg ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg
     2041 gcatatcatt tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta
     2101 ttgcggaaca aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca
     2161 aaataacatc aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac
     2221 catcagcgac aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattaac
     2281 atctgaagat atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt
     2341 cagcaacatg attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca
     2401 gagagaatta cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga
     2461 agaatattct tcatttacgt ctttttatgc agaaaaagca aatttattaa aaagaaacaa
     2521 atgttattta aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg
     2581 aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt
     2641 attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa
     2701 actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagaaagt
     2761 actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa
     2821 acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag
     2881 aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa
     2941 gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct
     3001 aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc
     3061 aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac
     3121 agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc
     3181 caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc
     3241 gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag
     3301 acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac
     3361 tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt
     3421 tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact
     3481 tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg
     3541 aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg
     3601 caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag tggagaccat
     3661 cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa
     3721 ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa
     3781 aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg
     3841 ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg
     3901 ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt
     3961 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
     4021 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
     4081 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt atctactgca
     4141 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
     4201 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
     4261 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
     4321 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
     4381 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
     4441 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
     4501 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
     4561 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
     4621 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcaagca ttgtgaaatg
     4681 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
     4741 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
     4801 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
     4861 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
     4921 agaagcgacg actgcacctt cttctgtcaa tatgaaacca tggcgctttg tggtcgctga
     4981 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
     5041 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
     5101 aatctacaac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
     5161 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
     5221 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
     5281 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
     5341 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
     5401 gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa taaaaggttt tcagttacta
     5461 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
     5521 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
     5581 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
     5641 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
     5701 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
     5761 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
     5821 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
     5881 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
     5941 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
     6001 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
     6061 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
     6121 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
     6181 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
     6241 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
     6301 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
     6361 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
     6421 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
     6481 gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc ctctaaacaa
     6541 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     6601 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     6661 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     6721 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     6781 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     6841 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     6901 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     6961 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     7021 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     7081 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     7141 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     7201 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     7261 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     7321 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
     7381 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
     7441 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
     7501 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
     7561 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
     7621 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
     7681 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
     7741 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
     7801 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
     7861 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
     7921 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
     7981 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
     8041 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
     8101 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
     8161 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
     8221 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
     8281 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
     8341 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
     8401 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
     8461 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
     8521 acagtaaagt ttccacgttt tacatgtgca tattgacatg taaaataaat catgtatgat
     8581 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
     8641 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
     8701 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
     8761 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
     8821 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
     8881 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
     8941 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
     9001 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
     9061 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
     9121 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
     9181 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
     9241 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
     9301 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
     9361 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
     9421 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
     9481 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
     9541 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
     9601 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
     9661 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
     9721 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
     9781 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
     9841 ataagaa
//
LOCUS       DAJSNV010000301         3874 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.156, whole genome shotgun sequence.
ACCESSION   DAJSNV010000301 DAJSNV010000000
VERSION     DAJSNV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.156"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            117..614
                     /gene="dfrG"
                     /locus_tag="ON516_003116"
     CDS             117..614
                     /gene="dfrG"
                     /locus_tag="ON516_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR3346506.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(686..2638)
                     /locus_tag="ON516_003117"
     CDS             complement(686..2638)
                     /locus_tag="ON516_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR3346507.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2645..2881)
                     /locus_tag="ON516_003118"
     CDS             complement(2645..2881)
                     /locus_tag="ON516_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3346508.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3119..3874
                     /locus_tag="ON516_003119"
                     /pseudo
     CDS             3119..3874
                     /locus_tag="ON516_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1187 a          675 c          638 g         1374 t
ORIGIN      
        1 taatataact tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt
       61 tttttgatag aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga
      121 aagtttcttt gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc
      181 cttggaggat tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa
      241 tattaggtag gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta
      301 ttctgacgag agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag
      361 atgtttttga gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt
      421 ataatttgtt tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg
      481 aaggagatac tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa
      541 aagggataaa gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa
      601 acttattgag ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc
      661 ttgggattac tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt
      721 gtttatctgt aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa
      781 aagggcgaca aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg
      841 gattgaagtt agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt
      901 caagagatga tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa
      961 catttaatag catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat
     1021 caactgaata aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat
     1081 atttgcgata tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta
     1141 gtaattcagt tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga
     1201 tatctcccca atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc
     1261 tgcttttcgt cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt
     1321 tcgtgaatac tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc
     1381 attcgtcggc attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag
     1441 catcaacatt taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa
     1501 gataagcatt cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat
     1561 ctaacctatc atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat
     1621 cttcatttaa ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat
     1681 atcttgataa gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc
     1741 ccgtatattt ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct
     1801 tgtccgtatt aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc
     1861 gttctaaaaa ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa
     1921 ctccaattcc taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag
     1981 cgaacattgg aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg
     2041 gtagaacatt agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac
     2101 ttttccccct tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc
     2161 agccttcttt atgaaattga taatctggct caacatcttg gatgctattg atttctataa
     2221 ggcgtttaat ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg
     2281 atacacctct gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg
     2341 aactaccagt cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt
     2401 ctttcccaaa attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag
     2461 aaccgttcca tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc
     2521 gttttggagt taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg
     2581 gaattttaac atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg
     2641 tctatcaacc cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct
     2701 tgttggcaat ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca
     2761 ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt
     2821 gctctgatat ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca
     2881 taggaaaatg cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca
     2941 ttcctacaat aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg
     3001 tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa
     3061 tttgatactc atttaggttt acattttcac tttttgtact ccaaaactga actacttatt
     3121 tttatttagt tgtctctata ttttctctgc ttatcggaca gtagacatct actatcgttc
     3181 cgcaattgcc gaagctatcg caataacact tgttatccct gtattatttt atgcttatca
     3241 aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag ctagcattga gtatgagttt
     3301 gctagtgtac agccatgtac tatcaacatt aatgagtaca gcattaatta tcatttttat
     3361 ttttattcgt ttggtttcta aaggtttcaa gaatgcagat tttattgcta tatttaaaaa
     3421 attatttagt gcggcaggaa tgacattggt tctgacgagt gctttttggt atccgatgtt
     3481 tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg acaaatttat atgctcatgc
     3541 atctaatgtg ttcgatagtc tgactgaagc tatgaacaac gacttaacta cttactcaat
     3601 gggactagtt ggtctgttat ctttatgtat tcccctgatt ttatttaaaa aattaacaaa
     3661 catagaaaaa aagatttatt atggtacgtg tttgacttgg ctagctacaa ctagtttggt
     3721 gccatggtat cttctgcaaa atacgccagc caaattgtta caatttcctt ggcggatctt
     3781 aagcttgcaa ataatattct cttctctgat attaacgatg atatttttca aaaatagaag
     3841 atacaataag acaagagaac tattttattt attt
//
LOCUS       DAJSNV010000302         3067 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.168, whole genome shotgun sequence.
ACCESSION   DAJSNV010000302 DAJSNV010000000
VERSION     DAJSNV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.168"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            163..246
                     /locus_tag="ON516_003120"
     CDS             163..246
                     /locus_tag="ON516_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3346509.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            371..1108
                     /gene="erm(B)"
                     /locus_tag="ON516_003121"
     CDS             371..1108
                     /gene="erm(B)"
                     /locus_tag="ON516_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3346510.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1371..1487)
                     /locus_tag="ON516_003122"
     CDS             complement(1371..1487)
                     /locus_tag="ON516_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HCR3346511.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            1750..2262
                     /locus_tag="ON516_003123"
                     /pseudo
     CDS             1750..2262
                     /locus_tag="ON516_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2311..2991
                     /locus_tag="ON516_003124"
     CDS             2311..2991
                     /locus_tag="ON516_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3346512.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1042 a          509 c          566 g          950 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaaccagtt atggaaataa gacttagaag
       61 caaacttaag agtgtgttga tagtgcatta aaaattttgt ataataggaa ttgaagttaa
      121 attagatgct aaaaatttgt aattaagaag gagggattcg tcatgttggt attccaaatg
      181 cgttatgtag ataaaacatc tactgttttg aaacagacta aaaacagtga ttacgcagat
      241 aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt taaacagata
      301 actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta atcacttcag
      361 gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
      421 actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
      481 aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
      541 tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt
      601 cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
      661 tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
      721 ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
      781 tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
      841 gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
      901 ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
      961 ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
     1021 cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
     1081 ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt
     1141 tacacgttac taaagggaat gtagataaat tattaggtat agggcacctc taataactac
     1201 caattaattc acctcaaaat gaaattagaa aaaaacagcg aaatttttaa atctatttct
     1261 tatcgataca aattcctcgt aggcgctcgg gaccccaaca tgaggaaatg tttttataaa
     1321 aaaagagtag ttaccgtttt ttggtaacta ctcttttcct tttttaaggt ttaaatacct
     1381 ggaagtttag gtgttttggg tattggtggt gtaggtggct gcaagctttc caatttttgt
     1441 tgaattgctt taaattcagg ggtttcttgg tgttgatttt gaaccatttt ttgctctatt
     1501 ctctccaaag tcggttgaat ttctttccct gatactttac gatttaattc tctttctaag
     1561 gtctctttcg gactaatgga aggcgtttct aagcttgaat agagtttcac tccttctcta
     1621 ttgtaaagcc ttatatcgct aaataagccc gttttatgaa gggtttctaa attggtcggt
     1681 aagtttttga caacaatatc atgcgcttgt tttggtggtg ccctagctgt cattggatca
     1741 tctgcataca ataagatcca tgaaaggcca tctgctgcca atgagcgcct tgagattggt
     1801 aattgggaag ctgatactgt agccggtaaa acaggtaggg ctcgtattgt aactctagtc
     1861 gatcgtagat ctcgtttctc cctacttggt aaggttgaaa agaaaatctc taaaaatgtg
     1921 gttgattgta tgatcaatct actaagaaat gtaggagaag ataaatgtaa aaccgttaca
     1981 cccgatagag gaaaagaatt ctcccaacat agtcgaataa cgctagaatt aaatggcact
     2041 aaagtgtatt ttccagatcc acatgctcca tggcaacgtg gtacaaacga aaatactaat
     2101 ggtttattac gagaatattc acccaaaggt gaagatattt caggtatttt cgatagtcaa
     2161 attcaagaat gggcgaataa attaaacacg cgaccacgga aatgtctaaa ttggaaaacc
     2221 ccttatgaag tatttttggt tctgttgcaa agttccccat aactcctgat ttattgtaaa
     2281 atgtaagaaa acgagagtga gggtagacag atgaatcatt tcaaagggaa acaatttcaa
     2341 aaagatgtga ttattgtcgc tgttggttac tacctgcgtt acaatctgag ctatcgtgaa
     2401 gttcaggaat tactgtatga tcgcggaata aatgtttgtc acactacgat ttatcgttgg
     2461 gttcaagagt acagtaaaat cctctatcat ctttggaaga agaaaaatag acagtccttc
     2521 tactcgtgga aaatggacga gacctatatc aaaataaaag gccattggca ttatctctat
     2581 cgtgcgattg attctgatgg attaacttta gacatttggt tacgaaagaa acgggatact
     2641 caggcggctt atgctttctt aaaacggctc cataaacaat ttggacaacc tagagtgatt
     2701 gtaaccgata aagcaccatc gattagctct gcattcagaa agctacagat taacggttta
     2761 tataccaaga cagaacatcg gacagttaag tatctcaata atttaattga gcaggaccat
     2821 cgtccaatta agcgccgtaa taaattttat cgaagtttac gaactgcctc aaccacgatt
     2881 aagggcatgg agactattcg aggaatatac aaaaagaacc gaagaaacgg aacgctcttc
     2941 ggattttcgg tgtcgactga aattaaagtt ctgatgggga tcccagcata gtcaaaatat
     3001 taaactagga cttctatact cttttggggg ttctgttgca aagttttaaa taaagaataa
     3061 aatccct
//
LOCUS       DAJSNV010000303         1031 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00022860
            SAMN29549731-rid15779943.guided.252, whole genome shotgun sequence.
ACCESSION   DAJSNV010000303 DAJSNV010000000
VERSION     DAJSNV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549731
            Sequence Read Archive: SRR20032920
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474371.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:34:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,125
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,994
            CDSs (with protein)               :: 2,994
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 25 of 69
            Pseudo Genes (incomplete)         :: 38 of 69
            Pseudo Genes (internal stop)      :: 18 of 69
            Pseudo Genes (multiple problems)  :: 11 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549731-rid15779943.guided.252"
                     /strain="AUSMDU00022860"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            316..864
                     /gene="aac(6')"
                     /locus_tag="ON516_003125"
     CDS             316..864
                     /gene="aac(6')"
                     /locus_tag="ON516_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3346513.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          367 a          147 c          210 g          307 t
ORIGIN      
        1 tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc
       61 aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag
      121 ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta
      181 taattttttt attttataag taagataaat aatgaaagaa tagagatact gattggtagc
      241 taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa
      301 caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat
      361 cagctttctg atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa
      421 gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag
      481 ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca
      541 ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta
      601 gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac
      661 catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc
      721 atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta
      781 ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt
      841 attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag
      901 aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc
      961 cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg
     1021 agtagtaaga a
//
